LOCUS       DACERL010000001        92311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.001, whole genome shotgun sequence.
ACCESSION   DACERL010000001 DACERL010000000
VERSION     DACERL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92311
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.001"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(218..1015)
                     /locus_tag="IXF54_000001"
     CDS             complement(218..1015)
                     /locus_tag="IXF54_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1403293.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IXF54_000002"
     CDS             complement(1012..2196)
                     /locus_tag="IXF54_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1403294.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IXF54_000003"
     CDS             complement(2221..3468)
                     /locus_tag="IXF54_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1403295.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IXF54_000004"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IXF54_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1403296.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IXF54_000005"
     CDS             5448..5762
                     /locus_tag="IXF54_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1403297.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IXF54_000006"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IXF54_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1403298.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IXF54_000007"
     CDS             6447..7091
                     /locus_tag="IXF54_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1403299.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IXF54_000008"
     CDS             7106..7435
                     /locus_tag="IXF54_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403300.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IXF54_000009"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IXF54_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1403301.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IXF54_000010"
     CDS             8423..9247
                     /locus_tag="IXF54_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1403302.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IXF54_000011"
     CDS             9240..9587
                     /locus_tag="IXF54_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1403303.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IXF54_000012"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IXF54_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1403304.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IXF54_000013"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IXF54_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1403305.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IXF54_000014"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IXF54_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1403306.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IXF54_000015"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IXF54_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1403307.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IXF54_000016"
     CDS             15244..16011
                     /locus_tag="IXF54_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403308.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IXF54_000017"
     CDS             16001..18043
                     /locus_tag="IXF54_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403309.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IXF54_000018"
     CDS             18324..18473
                     /locus_tag="IXF54_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1403310.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IXF54_000019"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IXF54_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1403311.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IXF54_000020"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IXF54_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1403312.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IXF54_000021"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IXF54_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1403313.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IXF54_000022"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IXF54_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1403314.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IXF54_000023"
     CDS             22890..23972
                     /locus_tag="IXF54_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1403315.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IXF54_000024"
     CDS             23972..24775
                     /locus_tag="IXF54_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1403316.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IXF54_000025"
     CDS             24845..25585
                     /locus_tag="IXF54_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1403317.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IXF54_000026"
     CDS             25596..26600
                     /locus_tag="IXF54_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1403318.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IXF54_000027"
     CDS             complement(26645..28489)
                     /locus_tag="IXF54_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403319.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28701..29219
                     /locus_tag="IXF54_000028"
     CDS             28701..29219
                     /locus_tag="IXF54_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1403320.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29347..30030
                     /locus_tag="IXF54_000029"
     CDS             29347..30030
                     /locus_tag="IXF54_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1403321.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30339..30412
                     /locus_tag="IXF54_000030"
     tRNA            30339..30412
                     /locus_tag="IXF54_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30373..30375,aa:Arg,seq:tct)
     gene            30571..30930
                     /locus_tag="IXF54_000031"
     CDS             30571..30930
                     /locus_tag="IXF54_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1403322.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31053..32684
                     /locus_tag="IXF54_000032"
     CDS             31053..32684
                     /locus_tag="IXF54_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1403323.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32746..33387)
                     /locus_tag="IXF54_000033"
     CDS             complement(32746..33387)
                     /locus_tag="IXF54_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1403324.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33669..33977
                     /gene="rplU"
                     /locus_tag="IXF54_000034"
     CDS             33669..33977
                     /gene="rplU"
                     /locus_tag="IXF54_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1403325.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34004..34348
                     /locus_tag="IXF54_000035"
     CDS             34004..34348
                     /locus_tag="IXF54_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1403326.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34361..34654
                     /gene="rpmA"
                     /locus_tag="IXF54_000036"
     CDS             34361..34654
                     /gene="rpmA"
                     /locus_tag="IXF54_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1403327.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35061..37895
                     /locus_tag="IXF54_000037"
     CDS             35061..37895
                     /locus_tag="IXF54_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1403328.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38086..38577
                     /locus_tag="IXF54_000038"
     CDS             38086..38577
                     /locus_tag="IXF54_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1403329.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38680..39669
                     /gene="manX"
                     /locus_tag="IXF54_000039"
     CDS             38680..39669
                     /gene="manX"
                     /locus_tag="IXF54_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1403330.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39702..40505
                     /locus_tag="IXF54_000040"
     CDS             39702..40505
                     /locus_tag="IXF54_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1403331.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40526..41440
                     /locus_tag="IXF54_000041"
     CDS             40526..41440
                     /locus_tag="IXF54_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1403332.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41523..41897
                     /locus_tag="IXF54_000042"
     CDS             41523..41897
                     /locus_tag="IXF54_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1403333.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42036..42281
                     /locus_tag="IXF54_000043"
     CDS             42036..42281
                     /locus_tag="IXF54_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1403334.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42453..43517
                     /locus_tag="IXF54_000044"
     CDS             42453..43517
                     /locus_tag="IXF54_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1403335.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43763..44188
                     /locus_tag="IXF54_000045"
     CDS             43763..44188
                     /locus_tag="IXF54_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1403336.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44181..44630
                     /gene="nusB"
                     /locus_tag="IXF54_000046"
     CDS             44181..44630
                     /gene="nusB"
                     /locus_tag="IXF54_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1403337.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44727..45581
                     /locus_tag="IXF54_000047"
     CDS             44727..45581
                     /locus_tag="IXF54_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1403338.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45582..46919
                     /locus_tag="IXF54_000048"
     CDS             45582..46919
                     /locus_tag="IXF54_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1403339.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46924..47148
                     /locus_tag="IXF54_000049"
     CDS             46924..47148
                     /locus_tag="IXF54_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1403340.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47150..48040
                     /locus_tag="IXF54_000050"
     CDS             47150..48040
                     /locus_tag="IXF54_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1403341.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48049..48867
                     /locus_tag="IXF54_000051"
     CDS             48049..48867
                     /locus_tag="IXF54_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1403342.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48974..49423
                     /locus_tag="IXF54_000052"
     CDS             48974..49423
                     /locus_tag="IXF54_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1403343.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49441..51123
                     /gene="recN"
                     /locus_tag="IXF54_000053"
     CDS             49441..51123
                     /gene="recN"
                     /locus_tag="IXF54_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1403344.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51178..51294)
                     /locus_tag="IXF54_000054"
     CDS             complement(51178..51294)
                     /locus_tag="IXF54_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1403345.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51367..52323)
                     /locus_tag="IXF54_000055"
     CDS             complement(51367..52323)
                     /locus_tag="IXF54_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1403346.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52494..52862
                     /locus_tag="IXF54_000056"
     CDS             52494..52862
                     /locus_tag="IXF54_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1403347.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53032..53463
                     /gene="mraZ"
                     /locus_tag="IXF54_000057"
     CDS             53032..53463
                     /gene="mraZ"
                     /locus_tag="IXF54_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1403348.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53484..54443
                     /gene="rsmH"
                     /locus_tag="IXF54_000058"
     CDS             53484..54443
                     /gene="rsmH"
                     /locus_tag="IXF54_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1403349.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54448..54849
                     /gene="ftsL"
                     /locus_tag="IXF54_000059"
     CDS             54448..54849
                     /gene="ftsL"
                     /locus_tag="IXF54_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1403350.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54850..57042
                     /locus_tag="IXF54_000060"
     CDS             54850..57042
                     /locus_tag="IXF54_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1403351.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYPYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57068..58030
                     /locus_tag="IXF54_000061"
     CDS             57068..58030
                     /locus_tag="IXF54_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1403352.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58044..59405
                     /locus_tag="IXF54_000062"
     CDS             58044..59405
                     /locus_tag="IXF54_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1403353.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59431..60519
                     /gene="murG"
                     /locus_tag="IXF54_000063"
     CDS             59431..60519
                     /gene="murG"
                     /locus_tag="IXF54_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1403354.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60534..61754
                     /locus_tag="IXF54_000064"
     CDS             60534..61754
                     /locus_tag="IXF54_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1403355.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61910..63235
                     /gene="ftsA"
                     /locus_tag="IXF54_000065"
     CDS             61910..63235
                     /gene="ftsA"
                     /locus_tag="IXF54_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1403356.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63258..64499
                     /gene="ftsZ"
                     /locus_tag="IXF54_000066"
     CDS             63258..64499
                     /gene="ftsZ"
                     /locus_tag="IXF54_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1403357.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64516..65193
                     /locus_tag="IXF54_000067"
     CDS             64516..65193
                     /locus_tag="IXF54_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1403358.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65207..65806
                     /locus_tag="IXF54_000068"
     CDS             65207..65806
                     /locus_tag="IXF54_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1403359.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65821..66108
                     /locus_tag="IXF54_000069"
     CDS             65821..66108
                     /locus_tag="IXF54_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1403360.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66157..66939
                     /locus_tag="IXF54_000070"
     CDS             66157..66939
                     /locus_tag="IXF54_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1403361.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66960..67667
                     /locus_tag="IXF54_000071"
     CDS             66960..67667
                     /locus_tag="IXF54_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1403362.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67929..70727
                     /gene="ileS"
                     /locus_tag="IXF54_000072"
     CDS             67929..70727
                     /gene="ileS"
                     /locus_tag="IXF54_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1403363.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70776..72302)
                     /locus_tag="IXF54_000073"
     CDS             complement(70776..72302)
                     /locus_tag="IXF54_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1403364.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72317..72964)
                     /locus_tag="IXF54_000074"
     CDS             complement(72317..72964)
                     /locus_tag="IXF54_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1403365.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73148..73477)
                     /locus_tag="IXF54_000075"
     CDS             complement(73148..73477)
                     /locus_tag="IXF54_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1403366.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73654..74382
                     /gene="sfsA"
                     /locus_tag="IXF54_000076"
     CDS             73654..74382
                     /gene="sfsA"
                     /locus_tag="IXF54_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1403367.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74398..75411
                     /locus_tag="IXF54_000077"
     CDS             74398..75411
                     /locus_tag="IXF54_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1403368.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75411..76688
                     /locus_tag="IXF54_000078"
     CDS             75411..76688
                     /locus_tag="IXF54_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1403369.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76751..79453
                     /locus_tag="IXF54_000079"
     CDS             76751..79453
                     /locus_tag="IXF54_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1403370.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79605..79922
                     /locus_tag="IXF54_000080"
     CDS             79605..79922
                     /locus_tag="IXF54_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1403371.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79952..80272
                     /locus_tag="IXF54_000081"
     CDS             79952..80272
                     /locus_tag="IXF54_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1403372.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80380..81840
                     /locus_tag="IXF54_000082"
     CDS             80380..81840
                     /locus_tag="IXF54_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1403373.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81908..82129
                     /locus_tag="IXF54_000083"
     CDS             81908..82129
                     /locus_tag="IXF54_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403374.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82160..82342
                     /locus_tag="IXF54_000084"
     CDS             82160..82342
                     /locus_tag="IXF54_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1403375.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82342..82755
                     /locus_tag="IXF54_000085"
     CDS             82342..82755
                     /locus_tag="IXF54_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1403376.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82878..84059
                     /locus_tag="IXF54_000086"
     CDS             82878..84059
                     /locus_tag="IXF54_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403377.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84448..84521
                     /locus_tag="IXF54_000087"
     tRNA            84448..84521
                     /locus_tag="IXF54_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84482..84484,aa:Arg,seq:cct)
     gene            complement(84590..85729)
                     /locus_tag="IXF54_000088"
     CDS             complement(84590..85729)
                     /locus_tag="IXF54_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1403378.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86028..86663)
                     /locus_tag="IXF54_000089"
     CDS             complement(86028..86663)
                     /locus_tag="IXF54_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403379.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86776..87411)
                     /locus_tag="IXF54_000090"
     CDS             complement(86776..87411)
                     /locus_tag="IXF54_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1403380.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87445..87906)
                     /locus_tag="IXF54_000091"
     CDS             complement(87445..87906)
                     /locus_tag="IXF54_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1403381.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(88036..88467)
                     /locus_tag="IXF54_000092"
     CDS             complement(88036..88467)
                     /locus_tag="IXF54_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1403382.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(88485..88805)
                     /locus_tag="IXF54_000093"
     CDS             complement(88485..88805)
                     /locus_tag="IXF54_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1403383.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            89104..89880
                     /locus_tag="IXF54_000094"
     CDS             89104..89880
                     /locus_tag="IXF54_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1403384.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            89895..90098
                     /locus_tag="IXF54_000095"
     CDS             89895..90098
                     /locus_tag="IXF54_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1403385.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            90114..90452
                     /locus_tag="IXF54_000096"
     CDS             90114..90452
                     /locus_tag="IXF54_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1403386.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            90439..90618
                     /locus_tag="IXF54_000097"
     CDS             90439..90618
                     /locus_tag="IXF54_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403387.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(90661..91131)
                     /locus_tag="IXF54_000098"
     CDS             complement(90661..91131)
                     /locus_tag="IXF54_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403388.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            91218..91916
                     /locus_tag="IXF54_000099"
     CDS             91218..91916
                     /locus_tag="IXF54_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1403389.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            92094..>92311
                     /locus_tag="IXF54_000100"
     CDS             92094..>92311
                     /locus_tag="IXF54_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403390.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYI"
BASE COUNT        30106 a        15722 c        19228 g        27255 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    29041 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    29101 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    29161 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    29221 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    29281 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    29341 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    29401 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    29461 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    29521 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    29581 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    29641 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    29701 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    29761 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    29821 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    29881 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    29941 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    30001 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    30061 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    30121 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    30181 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    30241 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    30301 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    30361 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    30421 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    30481 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    30541 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    30601 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    30661 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    30721 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    30781 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    30841 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    30901 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    30961 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    31021 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    31081 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    31141 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    31201 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    31261 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    31321 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    31381 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    31441 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    31501 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    31561 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    31621 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    31681 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    31741 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    31801 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    31861 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    31921 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    31981 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    32041 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    32101 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    32161 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    32221 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    32281 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    32341 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    32401 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    32461 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    32521 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    32581 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    32641 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    32701 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    32761 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    32821 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    32881 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    32941 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    33001 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    33061 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    33121 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    33181 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    33241 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    33301 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    33361 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    33421 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    33481 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    33541 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    33601 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    33661 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    33721 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    33781 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    33841 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    33901 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    33961 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    34021 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    34081 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    34141 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    34201 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    34261 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    34321 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    34381 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    34441 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    34501 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    34561 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    34621 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    34681 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    34741 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    34801 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    34861 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    34921 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    34981 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    35041 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    35101 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    35161 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    35221 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    35281 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    35341 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    35401 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    35461 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    35521 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    35581 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    35641 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    35701 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    35761 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    35821 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    35881 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    35941 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    36001 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    36061 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    36121 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    36181 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    36241 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    36301 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    36361 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    36421 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    36481 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    36541 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    36601 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    36661 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    36721 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    36781 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    36841 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    36901 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    36961 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    37021 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    37081 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    37141 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    37201 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    37261 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    37321 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    37381 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    37441 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    37501 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    37561 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    37621 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    37681 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    37741 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    37801 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    37861 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaatgat acacttttat
    37921 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    37981 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    38041 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    38101 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    38161 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    38221 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    38281 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    38341 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    38401 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    38461 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    38521 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    38581 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    38641 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    38701 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    38761 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    38821 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    38881 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    38941 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    39001 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    39061 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    39121 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    39181 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    39241 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    39301 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    39361 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    39421 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    39481 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    39541 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    39601 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    39661 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    39721 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    39781 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    39841 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    39901 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    39961 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    40021 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    40081 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    40141 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    40201 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    40261 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    40321 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    40381 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    40441 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    40501 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    40561 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    40621 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    40681 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    40741 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    40801 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    40861 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    40921 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    40981 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    41041 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    41101 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    41161 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    41221 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    41281 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    41341 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    41401 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    41461 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    41521 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    41581 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    41641 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    41701 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    41761 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    41821 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    41881 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    41941 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    42001 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    42061 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    42121 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    42181 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    42241 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    42301 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    42361 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    42421 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    42481 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    42541 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    42601 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    42661 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    42721 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    42781 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    42841 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    42901 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    42961 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    43021 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    43081 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    43141 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    43201 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    43261 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    43321 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    43381 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    43441 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    43501 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    43561 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    43621 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    43681 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    43741 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    43801 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    43861 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    43921 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    43981 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    44041 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    44101 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    44161 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    44221 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    44281 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    44341 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    44401 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    44461 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    44521 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    44581 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    44641 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    44701 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    44761 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    44821 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    44881 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    44941 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    45001 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    45061 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    45121 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    45181 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    45241 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    45301 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    45361 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    45421 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    45481 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    45541 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    45601 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    45661 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    45721 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    45781 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    45841 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    45901 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    45961 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    46021 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    46081 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    46141 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    46201 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    46261 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    46321 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    46381 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    46441 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    46501 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    46561 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    46621 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    46681 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    46741 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    46801 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    46861 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    46921 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    46981 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    47041 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    47101 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    47161 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    47221 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    47281 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    47341 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    47401 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    47461 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    47521 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    47581 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    47641 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    47701 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    47761 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    47821 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    47881 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    47941 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    48001 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    48061 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    48121 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    48181 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    48241 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    48301 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    48361 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    48421 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    48481 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    48541 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    48601 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    48661 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    48721 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    48781 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    48841 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    48901 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    48961 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    49021 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    49081 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    49141 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    49201 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    49261 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    49321 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    49381 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    49441 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    49501 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    49561 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    49621 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    49681 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    49741 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    49801 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    49861 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    49921 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    49981 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    50041 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    50101 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    50161 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    50221 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    50281 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    50341 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    50401 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    50461 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    50521 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    50581 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    50641 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    50701 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    50761 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    50821 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    50881 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    50941 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    51001 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    51061 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    51121 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    51181 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    51241 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    51301 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    51361 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    51421 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    51481 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    51541 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    51601 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    51661 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    51721 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    51781 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    51841 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    51901 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    51961 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    52021 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    52081 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    52141 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    52201 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    52261 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    52321 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    52381 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    52441 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    52501 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    52561 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    52621 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    52681 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    52741 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    52801 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    52861 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    52921 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    52981 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    53041 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    53101 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    53161 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    53221 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    53281 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    53341 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    53401 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    53461 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    53521 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    53581 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    53641 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    53701 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    53761 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    53821 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    53881 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    53941 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    54001 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    54061 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    54121 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    54181 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    54241 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    54301 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    54361 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    54421 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    54481 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    54541 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    54601 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    54661 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    54721 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    54781 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    54841 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    54901 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    54961 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    55021 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    55081 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    55141 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    55201 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    55261 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    55321 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    55381 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    55441 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    55501 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    55561 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    55621 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    55681 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    55741 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    55801 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    55861 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    55921 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    55981 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    56041 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    56101 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    56161 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    56221 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    56281 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    56341 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    56401 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc
    56461 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacccg
    56521 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    56581 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    56641 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    56701 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    56761 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    56821 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    56881 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    56941 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    57001 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    57061 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    57121 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    57181 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    57241 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    57301 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    57361 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    57421 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    57481 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    57541 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    57601 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    57661 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    57721 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    57781 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    57841 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    57901 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    57961 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    58021 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    58081 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    58141 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    58201 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    58261 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    58321 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    58381 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    58441 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    58501 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    58561 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    58621 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    58681 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    58741 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    58801 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    58861 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    58921 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    58981 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    59041 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    59101 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    59161 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    59221 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    59281 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    59341 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    59401 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    59461 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    59521 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    59581 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    59641 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    59701 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    59761 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    59821 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    59881 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    59941 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    60001 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    60061 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    60121 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    60181 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    60241 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    60301 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    60361 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    60421 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    60481 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    60541 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    60601 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    60661 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    60721 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    60781 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    60841 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    60901 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    60961 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    61021 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    61081 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    61141 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    61201 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    61261 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    61321 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    61381 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    61441 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    61501 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    61561 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    61621 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    61681 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    61741 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    61801 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    61861 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    61921 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    61981 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    62041 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    62101 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    62161 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    62221 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    62281 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    62341 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    62401 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    62461 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    62521 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    62581 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    62641 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    62701 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    62761 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    62821 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    62881 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    62941 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    63001 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    63061 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    63121 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    63181 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    63241 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    63301 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    63361 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    63421 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    63481 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    63541 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    63601 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    63661 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    63721 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    63781 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    63841 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    63901 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    63961 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    64021 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    64081 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    64141 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    64201 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    64261 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    64321 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    64381 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    64441 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    64501 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    64561 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    64621 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    64681 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    64741 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    64801 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    64861 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    64921 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    64981 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    65041 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    65101 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    65161 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    65221 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    65281 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    65341 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    65401 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    65461 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    65521 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    65581 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    65641 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    65701 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    65761 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    65821 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    65881 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    65941 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    66001 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    66061 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    66121 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    66181 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    66241 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    66301 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    66361 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    66421 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    66481 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    66541 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    66601 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    66661 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    66721 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    66781 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    66841 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    66901 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    66961 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    67021 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    67081 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    67141 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    67201 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    67261 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    67321 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    67381 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    67441 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    67501 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    67561 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    67621 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    67681 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    67741 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    67801 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    67861 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    67921 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    67981 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    68041 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    68101 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    68161 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    68221 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    68281 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    68341 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    68401 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    68461 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    68521 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    68581 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    68641 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    68701 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    68761 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    68821 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    68881 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    68941 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    69001 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    69061 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    69121 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    69181 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    69241 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    69301 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    69361 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    69421 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    69481 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    69541 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    69601 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    69661 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    69721 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    69781 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    69841 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    69901 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    69961 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    70021 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    70081 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    70141 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    70201 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    70261 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    70321 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    70381 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    70441 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    70501 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    70561 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    70621 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    70681 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    70741 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    70801 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    70861 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    70921 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    70981 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    71041 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    71101 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    71161 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    71221 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    71281 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    71341 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    71401 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    71461 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    71521 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    71581 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    71641 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    71701 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    71761 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    71821 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    71881 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    71941 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    72001 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    72061 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    72121 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    72181 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    72241 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    72301 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    72361 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    72421 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    72481 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    72541 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    72601 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    72661 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    72721 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    72781 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    72841 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    72901 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    72961 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    73021 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    73081 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    73141 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    73201 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    73261 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    73321 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    73381 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    73441 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    73501 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    73561 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    73621 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    73681 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    73741 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    73801 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    73861 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    73921 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    73981 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    74041 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    74101 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    74161 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    74221 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    74281 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    74341 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    74401 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    74461 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    74521 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    74581 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    74641 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    74701 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    74761 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    74821 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    74881 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    74941 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    75001 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    75061 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    75121 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    75181 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    75241 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    75301 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    75361 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    75421 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    75481 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    75541 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    75601 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    75661 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    75721 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    75781 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    75841 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    75901 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    75961 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    76021 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    76081 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    76141 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    76201 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    76261 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    76321 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    76381 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    76441 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    76501 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    76561 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    76621 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    76681 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    76741 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    76801 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    76861 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    76921 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    76981 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    77041 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    77101 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    77161 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    77221 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    77281 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    77341 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    77401 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    77461 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    77521 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    77581 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    77641 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    77701 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    77761 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    77821 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    77881 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    77941 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    78001 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    78061 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    78121 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    78181 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    78241 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    78301 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    78361 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    78421 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    78481 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    78541 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    78601 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    78661 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    78721 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    78781 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    78841 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    78901 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    78961 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    79021 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    79081 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    79141 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    79201 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    79261 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    79321 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    79381 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    79441 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    79501 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    79561 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    79621 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    79681 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    79741 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    79801 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    79861 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    79921 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    79981 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    80041 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    80101 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    80161 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    80221 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    80281 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    80341 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    80401 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    80461 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    80521 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    80581 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    80641 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    80701 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    80761 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    80821 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    80881 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    80941 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    81001 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    81061 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    81121 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    81181 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    81241 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    81301 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    81361 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    81421 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    81481 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    81541 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    81601 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    81661 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    81721 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    81781 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    81841 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    81901 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    81961 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    82021 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    82081 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    82141 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    82201 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    82261 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    82321 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    82381 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    82441 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    82501 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    82561 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    82621 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    82681 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    82741 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    82801 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    82861 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    82921 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    82981 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    83041 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    83101 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    83161 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    83221 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    83281 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    83341 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    83401 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    83461 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    83521 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
    83581 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
    83641 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
    83701 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
    83761 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
    83821 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
    83881 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
    83941 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
    84001 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
    84061 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
    84121 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
    84181 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
    84241 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
    84301 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
    84361 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
    84421 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
    84481 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
    84541 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
    84601 ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc
    84661 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
    84721 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
    84781 catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc
    84841 tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
    84901 ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
    84961 caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc
    85021 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
    85081 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta
    85141 acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag
    85201 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
    85261 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
    85321 acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat
    85381 acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca
    85441 tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg
    85501 ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc
    85561 gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt
    85621 ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
    85681 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag
    85741 ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt
    85801 tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata
    85861 attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa
    85921 aagaataact gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt
    85981 tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt
    86041 gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca
    86101 tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt
    86161 aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt
    86221 tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc
    86281 agtacaagaa gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa
    86341 ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg
    86401 tcatttatcc cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct
    86461 tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg
    86521 atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca
    86581 atataaggaa ttgctccaat accgccttca actattaatt caagatattc ttttttggtt
    86641 agtttttcat tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt
    86701 gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag
    86761 tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc attgagcata
    86821 ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc
    86881 ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg
    86941 tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat
    87001 tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata
    87061 tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa
    87121 agcaccccag tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt
    87181 gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc
    87241 atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat
    87301 aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat
    87361 taaacctata ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc
    87421 ctctttctct tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa
    87481 ttcttcttga ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc
    87541 acttgaaatt tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata
    87601 tttttttaaa ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc
    87661 tttcgtggcc gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat
    87721 ataaccatta ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat
    87781 agttttttct cctttaatac cttgattaac taaatttata aatccttttc taataattga
    87841 aatagaatca tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt
    87901 ttccattttt tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag
    87961 gaaagagccc gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa
    88021 taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca
    88081 ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat actccataaa
    88141 tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa
    88201 aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt
    88261 atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa
    88321 atgaaaggct tctgcagttt caccgtatag attttcattc aaaaaaatga ttctgggttt
    88381 tggatagtag aaaccaggct ctttcatctc catataaact actttcaaat tgtattcgct
    88441 caacatttct ttcaatttca aatacataca aaccatcact ccaactattc attttcctct
    88501 aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca
    88561 aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt taggggtttc tctttcttct
    88621 ctacctagaa ggtagtctac agagacatcg aaatagtcag caatccgact taattcatct
    88681 gaattaggag tattattttt ccactttgct agataaccgt tagaatatcc aaaattaatt
    88741 tctaactgcc gtatagatat ctttttcctc ttcgccaagt cttttattat ttcataggta
    88801 ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa
    88861 ataatttgac taattctgag taatgagcta tactatatct tgtaaacaga tttaacaact
    88921 aaaaagacaa caaaaaacac tattgatatg taaatgcaga ccgccaagaa agctttaaaa
    88981 tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact cagatactgt
    89041 caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga
    89101 aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac aattttagta
    89161 aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta ttcaaacact
    89221 aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg aatcacgacc
    89281 tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag tttaatctta
    89341 aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga agtgttgcca
    89401 gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc tttgcttaat
    89461 ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg aagattaatc
    89521 gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag catattatct
    89581 tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc tccacaacag
    89641 atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca atggttattg
    89701 tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat cccgaaagcc
    89761 gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg tctatttatc
    89821 tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga aattggttag
    89881 aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca
    89941 aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa
    90001 ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca
    90061 gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag ctaatgcaat
    90121 atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa gtggattatg
    90181 aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa gtgctagaac
    90241 ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca agatatagaa
    90301 atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt gaaggaagag
    90361 acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat ttttcggaaa
    90421 tcttaaggag gcaagcggat gggaaaattt aatagagcgc tagtgttcag cgcaccgcta
    90481 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
    90541 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
    90601 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
    90661 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
    90721 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
    90781 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
    90841 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
    90901 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
    90961 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
    91021 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
    91081 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
    91141 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
    91201 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
    91261 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
    91321 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
    91381 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
    91441 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
    91501 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
    91561 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
    91621 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
    91681 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
    91741 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
    91801 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
    91861 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
    91921 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
    91981 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
    92041 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
    92101 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
    92161 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
    92221 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
    92281 cgaagcattc gaataaacct gaatatattt a
//
LOCUS       DACERL010000002        77358 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.002, whole genome shotgun sequence.
ACCESSION   DACERL010000002 DACERL010000000
VERSION     DACERL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77358
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.002"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(524..751)
                     /locus_tag="IXF54_000101"
     CDS             complement(524..751)
                     /locus_tag="IXF54_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1403391.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="IXF54_000102"
     CDS             complement(760..1233)
                     /locus_tag="IXF54_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1403392.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="IXF54_000103"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="IXF54_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1403393.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="IXF54_000104"
     CDS             3042..3230
                     /locus_tag="IXF54_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1403394.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="IXF54_000105"
     CDS             3243..3800
                     /locus_tag="IXF54_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1403395.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="IXF54_000106"
     CDS             3832..4074
                     /locus_tag="IXF54_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1403396.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="IXF54_000107"
     CDS             4415..5629
                     /locus_tag="IXF54_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1403397.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..5878
                     /locus_tag="IXF54_000108"
                     /pseudo
     CDS             5660..5878
                     /locus_tag="IXF54_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6297..6770
                     /gene="dfrF"
                     /locus_tag="IXF54_000109"
     CDS             6297..6770
                     /gene="dfrF"
                     /locus_tag="IXF54_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1403398.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6897..7553
                     /locus_tag="IXF54_000110"
     CDS             6897..7553
                     /locus_tag="IXF54_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1403399.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7735..8265
                     /locus_tag="IXF54_000111"
     CDS             7735..8265
                     /locus_tag="IXF54_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1403400.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8811..9053
                     /locus_tag="IXF54_000112"
     CDS             8811..9053
                     /locus_tag="IXF54_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1403401.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9050..9520
                     /locus_tag="IXF54_000113"
     CDS             9050..9520
                     /locus_tag="IXF54_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1403402.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9648..10862
                     /locus_tag="IXF54_000114"
     CDS             9648..10862
                     /locus_tag="IXF54_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1403403.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(11572..11760)
                     /locus_tag="IXF54_000115"
     CDS             complement(11572..11760)
                     /locus_tag="IXF54_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403404.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            11851..12060
                     /locus_tag="IXF54_000116"
     CDS             11851..12060
                     /locus_tag="IXF54_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1403405.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12172..13785
                     /locus_tag="IXF54_000117"
     CDS             12172..13785
                     /locus_tag="IXF54_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1403406.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13820..15031
                     /locus_tag="IXF54_000118"
                     /pseudo
     CDS             13820..15031
                     /locus_tag="IXF54_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15054..15980
                     /gene="glsA"
                     /locus_tag="IXF54_000119"
     CDS             15054..15980
                     /gene="glsA"
                     /locus_tag="IXF54_000119"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1403407.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16188..17648
                     /locus_tag="IXF54_000120"
     CDS             16188..17648
                     /locus_tag="IXF54_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403408.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17869..19632
                     /locus_tag="IXF54_000121"
     CDS             17869..19632
                     /locus_tag="IXF54_000121"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1403409.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19716..20174
                     /locus_tag="IXF54_000122"
     CDS             19716..20174
                     /locus_tag="IXF54_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1403410.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20283..20966
                     /locus_tag="IXF54_000123"
     CDS             20283..20966
                     /locus_tag="IXF54_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1403411.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            21113..21727
                     /locus_tag="IXF54_000124"
     CDS             21113..21727
                     /locus_tag="IXF54_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1403412.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            21743..21949
                     /locus_tag="IXF54_000125"
     CDS             21743..21949
                     /locus_tag="IXF54_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403413.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            22490..23620
                     /locus_tag="IXF54_000126"
     CDS             22490..23620
                     /locus_tag="IXF54_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1403414.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            23712..24467
                     /locus_tag="IXF54_000127"
     CDS             23712..24467
                     /locus_tag="IXF54_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1403415.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            24525..26564
                     /locus_tag="IXF54_000128"
     CDS             24525..26564
                     /locus_tag="IXF54_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1403416.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(26673..27824)
                     /locus_tag="IXF54_000129"
     CDS             complement(26673..27824)
                     /locus_tag="IXF54_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1403417.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(27978..28508)
                     /locus_tag="IXF54_000130"
     CDS             complement(27978..28508)
                     /locus_tag="IXF54_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403418.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(28607..28957)
                     /locus_tag="IXF54_000131"
     CDS             complement(28607..28957)
                     /locus_tag="IXF54_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1403419.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IXF54_000132"
     CDS             complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IXF54_000132"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1403420.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IXF54_000133"
     CDS             complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IXF54_000133"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1403421.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(31360..32022)
                     /locus_tag="IXF54_000134"
     CDS             complement(31360..32022)
                     /locus_tag="IXF54_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1403422.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            32206..33852
                     /locus_tag="IXF54_000135"
     CDS             32206..33852
                     /locus_tag="IXF54_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1403423.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            33843..34925
                     /gene="uxuA"
                     /locus_tag="IXF54_000136"
     CDS             33843..34925
                     /gene="uxuA"
                     /locus_tag="IXF54_000136"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1403424.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(35033..36586)
                     /locus_tag="IXF54_000137"
                     /pseudo
     CDS             complement(35033..36586)
                     /locus_tag="IXF54_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            36734..37875
                     /locus_tag="IXF54_000138"
                     /pseudo
     CDS             36734..37875
                     /locus_tag="IXF54_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(38001..39080)
                     /locus_tag="IXF54_000139"
     CDS             complement(38001..39080)
                     /locus_tag="IXF54_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1403425.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(39183..39623)
                     /locus_tag="IXF54_000140"
     CDS             complement(39183..39623)
                     /locus_tag="IXF54_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1403426.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(39680..40090)
                     /locus_tag="IXF54_000141"
     CDS             complement(39680..40090)
                     /locus_tag="IXF54_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1403427.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(40270..40704)
                     /locus_tag="IXF54_000142"
     CDS             complement(40270..40704)
                     /locus_tag="IXF54_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1403428.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(40804..41652)
                     /locus_tag="IXF54_000143"
     CDS             complement(40804..41652)
                     /locus_tag="IXF54_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1403429.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            42041..43807
                     /locus_tag="IXF54_000144"
     CDS             42041..43807
                     /locus_tag="IXF54_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1403430.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            43909..45168
                     /locus_tag="IXF54_000145"
     CDS             43909..45168
                     /locus_tag="IXF54_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1403431.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            45315..46616
                     /locus_tag="IXF54_000146"
     CDS             45315..46616
                     /locus_tag="IXF54_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1403432.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            46619..47470
                     /locus_tag="IXF54_000147"
     CDS             46619..47470
                     /locus_tag="IXF54_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1403433.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            47483..48298
                     /locus_tag="IXF54_000148"
     CDS             47483..48298
                     /locus_tag="IXF54_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1403434.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            48449..49615
                     /locus_tag="IXF54_000149"
     CDS             48449..49615
                     /locus_tag="IXF54_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1403435.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            49845..50492
                     /locus_tag="IXF54_000150"
     CDS             49845..50492
                     /locus_tag="IXF54_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1403436.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            50576..51520
                     /locus_tag="IXF54_000151"
     CDS             50576..51520
                     /locus_tag="IXF54_000151"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1403437.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            51655..53106
                     /locus_tag="IXF54_000152"
     CDS             51655..53106
                     /locus_tag="IXF54_000152"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1403438.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            53127..53963
                     /locus_tag="IXF54_000153"
     CDS             53127..53963
                     /locus_tag="IXF54_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1403439.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            53963..54622
                     /locus_tag="IXF54_000154"
     CDS             53963..54622
                     /locus_tag="IXF54_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1403440.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            54748..55437
                     /locus_tag="IXF54_000155"
     CDS             54748..55437
                     /locus_tag="IXF54_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403441.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            55443..56399
                     /locus_tag="IXF54_000156"
     CDS             55443..56399
                     /locus_tag="IXF54_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1403442.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            56443..56955
                     /locus_tag="IXF54_000157"
     CDS             56443..56955
                     /locus_tag="IXF54_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403443.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(57211..57759)
                     /locus_tag="IXF54_000158"
     CDS             complement(57211..57759)
                     /locus_tag="IXF54_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1403444.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(57848..59029)
                     /locus_tag="IXF54_000159"
     CDS             complement(57848..59029)
                     /locus_tag="IXF54_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1403445.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(59265..60773)
                     /locus_tag="IXF54_000160"
     CDS             complement(59265..60773)
                     /locus_tag="IXF54_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1403446.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            60932..61123
                     /locus_tag="IXF54_000161"
     CDS             60932..61123
                     /locus_tag="IXF54_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403447.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            61326..62243
                     /locus_tag="IXF54_000162"
     CDS             61326..62243
                     /locus_tag="IXF54_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1403448.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            62236..63309
                     /locus_tag="IXF54_000163"
     CDS             62236..63309
                     /locus_tag="IXF54_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403449.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            63457..63975
                     /locus_tag="IXF54_000164"
     CDS             63457..63975
                     /locus_tag="IXF54_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403450.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            63956..64348
                     /locus_tag="IXF54_000165"
     CDS             63956..64348
                     /locus_tag="IXF54_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1403451.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            64424..65359
                     /locus_tag="IXF54_000166"
     CDS             64424..65359
                     /locus_tag="IXF54_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1403452.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            65492..66271
                     /locus_tag="IXF54_000167"
     CDS             65492..66271
                     /locus_tag="IXF54_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1403453.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            66264..66581
                     /locus_tag="IXF54_000168"
     CDS             66264..66581
                     /locus_tag="IXF54_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1403454.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            66825..68435
                     /locus_tag="IXF54_000169"
     CDS             66825..68435
                     /locus_tag="IXF54_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1403455.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            68592..69440
                     /locus_tag="IXF54_000170"
     CDS             68592..69440
                     /locus_tag="IXF54_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1403456.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            69671..70150
                     /locus_tag="IXF54_000171"
     CDS             69671..70150
                     /locus_tag="IXF54_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403457.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            70183..72411
                     /locus_tag="IXF54_000172"
     CDS             70183..72411
                     /locus_tag="IXF54_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1403458.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            72540..74183
                     /locus_tag="IXF54_000173"
     CDS             72540..74183
                     /locus_tag="IXF54_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1403459.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(74256..74801)
                     /locus_tag="IXF54_000174"
     CDS             complement(74256..74801)
                     /locus_tag="IXF54_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1403460.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(74824..75309)
                     /locus_tag="IXF54_000175"
     CDS             complement(74824..75309)
                     /locus_tag="IXF54_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1403461.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(75299..76111)
                     /locus_tag="IXF54_000176"
     CDS             complement(75299..76111)
                     /locus_tag="IXF54_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1403462.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(76250..>77358)
                     /locus_tag="IXF54_000177"
     CDS             complement(76250..>77358)
                     /locus_tag="IXF54_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1403463.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        26027 a        13423 c        15200 g        22708 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcgt ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt
     5881 ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat
     5941 attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg
     6001 aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt
     6061 caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag
     6121 taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat
     6181 gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga
     6241 tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga
     6301 taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat
     6361 ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta
     6421 tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg
     6481 tttccaccac aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg
     6541 cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg
     6601 aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg
     6661 gagatacatt ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac
     6721 ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga attgagtaaa
     6781 ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata aattgttgta
     6841 agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga ttgaatatga
     6901 ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag aaagatactt
     6961 ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa accttagtat
     7021 ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata aacgctgatg
     7081 gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt gaagatatag
     7141 ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat tatcaagagc
     7201 gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag attgcagact
     7261 tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta tgggaaagca
     7321 ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa
     7381 gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca atttggctga
     7441 ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt tcaagacatt
     7501 ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca taggcaaaaa
     7561 tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat ggttagtgat
     7621 tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat cagttgttcc
     7681 cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga aaaaatgaga
     7741 aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg taatggtgga
     7801 gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta tacagaattt
     7861 accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt tgttactgaa
     7921 ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac acataaagag
     7981 tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt attggaacgg
     8041 ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct tgttcagcag
     8101 ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac tctgttagga
     8161 aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct ttgtaaaaca
     8221 cagacttata acggaataac cgatttgatt tatatgcgta gataactaga gagaaacaga
     8281 aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct taagattttt
     8341 ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc gagccgttca
     8401 aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac actaacaagc
     8461 ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat aaattgtacc
     8521 tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc aaaaaataag
     8581 aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg tagttgtcgt
     8641 aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt tggagatgtt
     8701 caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga atgttataat
     8761 tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat atggaaaatg
     8821 aattactgga aaacttagga agacttcaca caactgaact tggcgttatt agaattaaaa
     8881 agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa attagccttt
     8941 ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat aattgtatta
     9001 taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa tgataaattt
     9061 ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac tgactaatta
     9121 tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca gtagtttgaa
     9181 aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg aaagaagaaa
     9241 tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag agggctttct
     9301 gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg ctatggataa
     9361 aatatatgaa acttatggca agatttatga agaaattatt cctagtacat cgtatattcc
     9421 tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt ggaatagtga
     9481 taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt atatccatac
     9541 acacaaagca gttagtctta ggattgactg ctttttctat acagattttt aatgacaggc
     9601 aattatcaat tatggtagtt gccttttttg attggaggaa ttgaagaatg aatgataaaa
     9661 actttattga agaactaaga caaaagcgtg aggaatacgg agtaacacaa acaaggcttg
     9721 ctgttgcctg tggtatcagc cgtgaatatt acaaccgcat tgaaaaagga aaacagccat
     9781 tgaatgacga attaagagaa atcatagaaa aacagattga gcgtttcaat ccgcaagaac
     9841 cactattctt attgattgat tactttcgtg tccgctttcc tactacggac gcattggcga
     9901 ttatccgtga tgtattacaa ctgaaatcta attacatgct ttatgaagat tatggaaaat
     9961 acggatatga aagcaaatat gtacttggcg acatcaatat catgtgttcc atgcaggagc
    10021 atttaggtgt tttattggaa ctgaaaggca aaggctgtcg gcaaatggaa tgttatctgc
    10081 tggcacagga acgctcatgg tatgacttca tgctggattg tatgacggct ggtggtgtga
    10141 tgaaacggct tgatttggct atcaatgata aagcaggaat attggatatt ccaaagttaa
    10201 aggaaaaata caaggctggc gaatgtatgt cctacttccg tatgcagaaa gattacagcg
    10261 gtacagaaaa atgcggtagc gatttaccaa agaatacagg agaaacctta tatctcggtt
    10321 caacaagtag cgaattatat atgtgtgctt atcagaaaaa ttatgagcag tatgtcaaga
    10381 atggcacaga aattgaagat acggagatta agaaccgttt tgaaatacgc atgaagaacg
    10441 aacgagccta ttatgcggtt gtagatttac tgacctatcg ggacgctgaa cgcaccgctt
    10501 tttctatcat caatcattat gtccgctttg ttgacagaga ggacgacaag ccaaaaagtc
    10561 aatggataac aaatgatgat tgggcatggt ttgtagggga aaacagagag ccgatacgct
    10621 taaccacaaa gccagagcct tacactttac aaaaagcgtt gcattggcta caaagacaag
    10681 tcgcaccaac cataaaaatg gtacaggcat tagacagaga aaatcgtaca acgatactga
    10741 aagatatgat tgagcaggca gaactaaaag acaagcataa acatttatta caattagaga
    10801 aatcaaccat agaagaacgt atagataccg ctgttccaca agagaatgac ggtatttttt
    10861 aatgtaattg aaaaatttca gatttagtca gcaaagagct gttttcattc cctatgtgaa
    10921 gtgaaagaag aaaaataaat tttttattaa atctgcaaca aaatggctac ttgcgtacag
    10981 atatggtgcg agaaaaggaa atctaaactt tttttgaaaa taggtcatta aatcggggct
    11041 tgctgtccct atatgaagtg aaagaagaaa aaagttttga aaaaatgacg gaaattcgtg
    11101 attagtggac agtcttctgt gaaaagagga aaaacatcat cattttgtat tcaaacaggc
    11161 attagcagtt gagtgttgat gtgaaaagat gaaaatactt tgttctcaac ttaacaattt
    11221 aaggtttccg ttccctatat ggtgcaaaga aaatatttca ttctattgtt ggcagtaacg
    11281 gtatggtttg ggtagttagg gtgtgaaaag gaaaatattc ttttttcatc atcaacttag
    11341 caaaatgaca ttttctatcc ctatatggtg catgaaaaga agttaaaatt tttccattta
    11401 aacttagcaa atggacgttt gctgtacaga tagtagtgaa aaaggagaaa atcaaccgtt
    11461 gattgccaaa atgaaccgtt ccatttgtac taataatgga aaaagttttg gattttggtt
    11521 acgtttctcc tcttttccaa cgcgatatat aggaaagacg atttttttct ttcaatcgtt
    11581 ctttgacaac tgaataaagc tattcaatac gtttgacaga cagctagccg ttgaaacaaa
    11641 tacgccacga cctttcccct gcaagagcga gcgaaaacct atttgtgttc ttgtaaaaga
    11701 tttgtcctct tttatcgcca tgacctgtac tgtcagataa tgatactccc gtacagccac
    11761 agcttgagca ttaagagcgt cgcacgcaat gggtacggct acataagaac tatgcagagg
    11821 tggaactcct gtgggctatg acaaaagccg ttgaattgct tagaaagatt ttacagaaag
    11881 ggggtatgtg ttattgataa tgctgtaaaa acaattcaaa agaaaaatgg caagtgtggc
    11941 attggcaaca gaggaaagac aaagattgta gtaaaagagc atttttccga aaagggcaaa
    12001 acaatggaag aacttctaac tgatgtcatg ctggaaaaag caaagcagac aatcgcataa
    12061 aatcggtgca gttgtaagaa atagattgaa tgacaaaaag tacccgtgtt atactttact
    12121 tgcaagcagg tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa
    12181 cagatttaca atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa
    12241 agttccagca ttaccacaca gagaagtatg ttgcgtttat atgcaaaaga acatcatttg
    12301 aatgtcattg atgaatatat tgatgacggc tggtcgggaa caaattttga cagaccgagt
    12361 tttcaaagaa tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac
    12421 ctttcccgtc ttggcagaaa ttatattatg acaggacaat atacagaatt gtatttccct
    12481 agtcataatg tccgctacat agcgattgat gacggtgtag acagcgaaaa aggcgaaagt
    12541 gagattgcac catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa
    12601 gtcaaatcag catttaagac aaagtttgcg gagggggctc attacggtgc ttatgctccg
    12661 ttaggatata agaaacaccc tgacatcaaa ggaaaactgt tggttgatga tgaaacaaaa
    12721 tggattattg aaaaaatctt ttccctagcc tatcaaggtt acggaagtgc aaaaatcaca
    12781 aagcagttaa gagcagaaaa agttccgaca gcgtcatggc taaattttac aagatacggt
    12841 acttttgcac atatctttga gggaaaaccc gaaagtaagc gttatgagtg gacgattgct
    12901 catgtcaagg cgatattgaa aagtgaggtt tatatcggaa acagtgttca caatatgcag
    12961 tctacggtat cgttcaagag caaaaagaaa gtgcgtaagc ccgaaagtga atggtttcga
    13021 gtagaaaaca ctcacgagcc gattattgac aaggaagtgt tctatcgtgt gcaggagcag
    13081 ataaaatcaa gacgcagaca gacaaaggaa aaggcaacgc cgatttttgc agggcttgtc
    13141 aagtgtgcgg attgtggttg gtctatgaga tttggaacga ataagacaaa taaaacgcct
    13201 tacagttatt atgcttgcag ttactacggg cagtttggca aaggtaattg ttctatgcac
    13261 tacattcgtt atgatgtgct gtatcaagcc gtattggaac gcttgcagta ttgggctaag
    13321 gcagtacagc aggacgaaga aaaggtattg aacaagatac agaaagctgg caatgcagag
    13381 agaatacggg aaaagaagaa aaaggcaagt acattgaaga aagccgagaa cagacaaaat
    13441 gagattgacc gtttatttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag
    13501 cgaaattttg tgatgttgtc cagcaagtac caaaaagaac agatagaact ggaacagcag
    13561 ataacaagcc taagagaaga actaagtaaa atggaacagg atatgatagg tgctgaaaag
    13621 tggattgagt taatcaagga atattccgta ccaaaggaac tgacagcacc attattaaat
    13681 gccatgatag aaaaaatcct catacatgaa gcaacaacga atgaggataa cgaaagaata
    13741 caggaaattg agatatatta ccgatttatt ggaagagttg agtaatcaac aagagtaata
    13801 tttttaacta agggaaaccg gcggtgtcta tacttgggtc agtcgcacat tgggaaaacg
    13861 attcggtttt gccgcaatct ttttccaatg gtttgagata acagttggct acttgaccat
    13921 gctgtatttc ttaactggtg cattatccta tgcaacgggt attggtgcta tacaaaacaa
    13981 taaatatctg aaattggcaa tcctattgat tattttctgg gctattttac tctcacagtt
    14041 aaaaggaacc aaatacactt cattgattgc tcggatcggt tttgtagctg gtatcttgtt
    14101 gccagcatta gtcttgtttg gcttagggat ccattatgtc gcttcaggcg ctccactaca
    14161 aacgatactg tctatgaaaa cattgattcc tgatttttcg aaattgccta cgctggttgt
    14221 ttttgtttcc tttattttag catatatggg cgtagaaaca tctgccagtc atgcaaatga
    14281 aatggaaaac cctaaaaagg attatccatt agcaatgttt gtattggtta ttctggcaat
    14341 cattttagat acttttggcg gattgaccgt cgctactaca gtacctcaac atggtttgtc
    14401 tcttaataca ggggtgattc aatcgttaga attcctatta agacacattg atccaaatct
    14461 tgcatggatt gcgaaaattc ttgcgattct tgtttgttta ggtgtcatcg gagaaattgc
    14521 ttcttgggta acctctcctt caaaagcact tcacgtagca gcagaagaag gattacttcc
    14581 tgaatatttt gcaaaagaaa acacacacgg tgttcctgct catttgatga tcgcaaatgg
    14641 gatcgttgca acaatctggg cagccgtctt tacattaagc ggcgggggga ataatctttc
    14701 atttcttgca gcgatgtcat tgactgttgt aatctacctg atgttgtact ttatgttttt
    14761 cgcgggctat ttgaagctgg tttttaaaga tgacagtctt gaaagagcct atcaggttcc
    14821 aggcggaaaa gttgtaaaag taatcgttgc ttgtttaggt cttttgactt cactttttgc
    14881 tttttgtatc tcatttgttc cacctgctag tatcgcagct ggacagcatg ggatttatga
    14941 aacgattctg attgtttctt atcttatcgt cttagttatt ccatttacga tctacgcatg
    15001 ccgtcataaa ttccagcatg cagaaaaata aaaaatgaaa gctggttatt caaatggaag
    15061 taaaagaatt agaacaattg attggaaaaa atttccacta ttatcaaaca ggacaagtgg
    15121 cggattacat tccagcatta gctgaagtgg atcccaaaca attaggtatg gcgatttatg
    15181 atttggatac aaaacaactg attgagggag gagacgccga tgtgcgcttt gccatcgaaa
    15241 gcatgtcaaa agttcccgta ttgttattag caatcaaaga caacggaata gaaaaagtct
    15301 ttcaaacaat caacacggaa ccaaccggtt ttgccttcaa ttctatcatg aatatggaaa
    15361 tcaatcatcg caaacatcca atgaatcctt ttgttaatgc aggagcgatt gcaacaacct
    15421 cgttgattgc cggtaaagat gcagatgaaa aatttgagcg aatcttgtca tttatgaaag
    15481 agatctgtga tgatcctaat atcacattga atgaagaaat ctatcattct gaatcaagaa
    15541 caggtgatat caatcgctct cttgcttact atatgaaagg taatcaaatg atcgaaggag
    15601 acgtaccgga gatactcgat gcttatttca gacaatgctc cctcaatgtc accgctacag
    15661 gcatagcaaa attagctgct gtgctggcaa ataaaggaat tgctccttgg aatggcaaac
    15721 aaatcattac tgaagaaagt gctacaatcg tcaaatcaat tatgacaact gctggtttgt
    15781 atgatgaatc aggagagttt tccgtccatg ttggtgtacc tgcaaaaagc ggtgtaggcg
    15841 gcgggttgat ggcagctgtg ccaaatcgtt acggcatagg tgtcttcagc ccggcactag
    15901 atccatttgg taacagcgca gctggtatcc aattgttgaa agatgtagtc aaagaactag
    15961 acgctgacat ctttgaataa ccattcttga aagaggctgg ggtaaaagtc caaacggctc
    16021 aaagctacct acaatgaggc tgaaattgtg agcttgtttg acttttgtcc cagtttcttt
    16081 ttttattggt ttttgaaagg gcttttctaa ataaaaagga aacaaaaata cgatttacag
    16141 atatgattaa ataaaatcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt
    16201 acatcgagcg aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag
    16261 aaatagacat cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg
    16321 atctagaaag tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc
    16381 ataagtataa actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt
    16441 acggtcaatc ttatttttta aagtttttaa gttattattt taatggtata ttcacgagta
    16501 cagaactggc tgatcttgaa tttatttctt tgagtaaagt atatacaatc aaaaaaatcg
    16561 tattggattt ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat
    16621 ttgactcgcg gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa
    16681 ataaaaatcc gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt
    16741 tttttaaaag aagatattcg gaagatgaaa agtcctttat catacgagaa atagagatcg
    16801 caatcggaag aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa
    16861 cgaagccgtt atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact
    16921 tacaagaaga agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg
    16981 agaacatgga gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg
    17041 acctatcact gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag
    17101 aaaattttat tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac
    17161 aagtattttt accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa
    17221 aaattgtaga aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg
    17281 aaaatttcat tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga
    17341 gacaggaaat agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa
    17401 cccaatttca tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt
    17461 cattagaaga attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg
    17521 ctgctagcta tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa
    17581 aaggtgcaaa catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata
    17641 tttcttgatg agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag
    17701 gtttttattt gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa
    17761 tttttttaaa atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa
    17821 caaatctatt ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta
    17881 ttacatgtat tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa
    17941 atgaaacagt tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt
    18001 gggcacgttg gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt
    18061 gattcaccaa aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt
    18121 ttaactggtc gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc
    18181 aaccaacatg aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc
    18241 ttagcaacag gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca
    18301 tttatcggtg acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt
    18361 gttttacaat ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat
    18421 catggcagtt tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat
    18481 aacctattta aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa
    18541 acggtgatcc gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc
    18601 catacggaaa aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg
    18661 catgtaccat ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt
    18721 aatatgatca tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc
    18781 gcagtcaatg cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca
    18841 gatcattatt ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg
    18901 gcttctcagg gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat
    18961 gatcaattat cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc
    19021 ggaacgatct cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca
    19081 agtatcccga acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta
    19141 gattgggcgt tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta
    19201 gaacatggta cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat
    19261 ggaggaaaag tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta
    19321 aaagctttta gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa
    19381 aatgacgtag aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa
    19441 gatggtagtt tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca
    19501 atgaacgtat taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag
    19561 ctttatgaac gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg
    19621 aatattcaat aattcattat gctcaccatc cagataattg tttatactag tatctggatg
    19681 gttttttcat tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg
    19741 tcaaaaagtt ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat
    19801 ttggggatac agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga
    19861 ctggtacatc aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg
    19921 gtcaattaga cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa
    19981 atgtgaccat cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag
    20041 gggatcggac attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata
    20101 aagaagcgtt gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag
    20161 taattttctt ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat
    20221 agaaaagggt tacattttta tatgaagctg tatttttata taacattact ggaggagata
    20281 ttgtggatac actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa
    20341 acgagccgct acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg
    20401 gagcagttgg tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg
    20461 atttaccggt aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg
    20521 cgacagaaaa agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg
    20581 caacttctcg taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg
    20641 aacacggtcg gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag
    20701 aagatggttt tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga
    20761 cgcagacacc tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa
    20821 ttatggaagg acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt
    20881 tttcagtcgt agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg
    20941 aagccgcaag taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag
    21001 aaaaattgat agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat
    21061 tttgtcttca agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga
    21121 acggagtcct tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat
    21181 ccctagacca gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc
    21241 cttcagttac ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag
    21301 aaaacaagat gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat
    21361 ccatatcctt gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa
    21421 tgaggaaagc gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt
    21481 actagcattg acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat
    21541 acggaataaa aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt
    21601 aaatgaaaca gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt
    21661 aagccgtttg gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc
    21721 cgaataaaaa ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa
    21781 agaaatgaaa aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt
    21841 tccgccattg caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga
    21901 aatgccagat caaacgatta gcggattgtt taattacttt aacaaataaa tatttattcc
    21961 gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa agaatcctgg
    22021 tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt tttctaaaaa
    22081 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
    22141 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
    22201 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
    22261 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
    22321 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
    22381 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
    22441 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
    22501 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
    22561 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
    22621 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
    22681 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
    22741 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
    22801 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
    22861 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
    22921 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
    22981 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
    23041 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
    23101 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
    23161 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
    23221 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
    23281 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
    23341 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
    23401 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
    23461 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
    23521 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
    23581 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
    23641 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
    23701 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
    23761 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
    23821 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
    23881 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
    23941 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
    24001 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
    24061 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
    24121 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
    24181 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
    24241 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
    24301 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
    24361 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
    24421 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
    24481 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
    24541 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
    24601 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
    24661 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
    24721 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
    24781 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
    24841 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
    24901 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
    24961 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
    25021 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
    25081 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
    25141 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
    25201 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
    25261 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
    25321 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
    25381 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
    25441 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
    25501 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
    25561 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
    25621 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
    25681 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
    25741 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
    25801 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
    25861 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
    25921 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
    25981 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
    26041 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
    26101 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
    26161 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
    26221 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
    26281 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
    26341 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
    26401 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
    26461 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
    26521 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
    26581 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
    26641 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
    26701 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
    26761 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
    26821 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
    26881 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
    26941 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
    27001 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
    27061 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
    27121 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
    27181 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
    27241 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
    27301 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
    27361 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
    27421 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
    27481 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
    27541 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
    27601 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
    27661 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
    27721 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
    27781 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
    27841 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
    27901 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
    27961 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
    28021 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
    28081 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
    28141 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
    28201 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
    28261 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
    28321 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
    28381 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
    28441 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
    28501 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
    28561 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
    28621 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
    28681 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
    28741 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
    28801 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
    28861 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
    28921 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
    28981 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
    29041 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
    29101 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
    29161 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
    29221 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
    29281 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
    29341 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
    29401 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
    29461 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
    29521 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
    29581 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
    29641 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
    29701 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
    29761 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
    29821 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
    29881 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
    29941 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
    30001 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
    30061 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
    30121 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
    30181 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
    30241 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
    30301 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
    30361 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
    30421 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
    30481 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
    30541 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
    30601 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
    30661 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
    30721 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
    30781 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
    30841 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
    30901 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
    30961 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
    31021 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
    31081 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
    31141 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
    31201 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
    31261 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
    31321 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
    31381 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
    31441 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
    31501 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
    31561 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
    31621 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
    31681 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
    31741 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
    31801 agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat acacgagttc
    31861 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
    31921 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
    31981 acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc ctccaaaatc
    32041 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
    32101 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    32161 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    32221 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    32281 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    32341 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    32401 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    32461 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    32521 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    32581 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    32641 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    32701 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    32761 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    32821 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    32881 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    32941 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    33001 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    33061 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    33121 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    33181 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    33241 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    33301 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    33361 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    33421 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    33481 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    33541 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    33601 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    33661 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    33721 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    33781 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    33841 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    33901 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    33961 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    34021 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    34081 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    34141 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    34201 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    34261 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    34321 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    34381 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    34441 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    34501 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    34561 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    34621 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    34681 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    34741 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    34801 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    34861 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    34921 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    34981 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    35041 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    35101 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    35161 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    35221 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    35281 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    35341 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    35401 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    35461 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    35521 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    35581 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    35641 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    35701 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    35761 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    35821 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    35881 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    35941 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    36001 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    36061 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    36121 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    36181 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    36241 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    36301 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    36361 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    36421 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    36481 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    36541 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    36601 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    36661 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    36721 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    36781 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    36841 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    36901 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    36961 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    37021 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    37081 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    37141 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    37201 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    37261 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    37321 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    37381 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    37441 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    37501 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    37561 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    37621 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    37681 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    37741 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    37801 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    37861 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    37921 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    37981 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    38041 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    38101 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    38161 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    38221 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    38281 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    38341 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    38401 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    38461 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    38521 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    38581 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    38641 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    38701 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    38761 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    38821 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    38881 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    38941 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    39001 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    39061 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    39121 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    39181 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    39241 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    39301 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    39361 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    39421 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    39481 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    39541 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    39601 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    39661 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    39721 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    39781 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    39841 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    39901 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    39961 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    40021 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    40081 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    40141 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    40201 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    40261 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    40321 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    40381 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    40441 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    40501 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    40561 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    40621 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    40681 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    40741 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    40801 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    40861 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    40921 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    40981 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    41041 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    41101 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    41161 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    41221 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    41281 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    41341 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    41401 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    41461 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    41521 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    41581 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    41641 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    41701 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    41761 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    41821 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    41881 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    41941 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    42001 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    42061 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    42121 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    42181 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    42241 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    42301 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    42361 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    42421 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    42481 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    42541 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    42601 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    42661 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    42721 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    42781 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    42841 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    42901 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    42961 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    43021 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    43081 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    43141 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    43201 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    43261 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    43321 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    43381 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    43441 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    43501 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    43561 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    43621 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    43681 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    43741 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    43801 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    43861 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    43921 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    43981 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    44041 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    44101 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    44161 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    44221 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    44281 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    44341 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    44401 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    44461 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    44521 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    44581 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    44641 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    44701 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    44761 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    44821 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    44881 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    44941 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    45001 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    45061 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    45121 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    45181 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    45241 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    45301 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    45361 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    45421 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    45481 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    45541 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    45601 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    45661 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    45721 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    45781 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    45841 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    45901 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    45961 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    46021 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    46081 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    46141 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    46201 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    46261 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    46321 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    46381 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    46441 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    46501 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    46561 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    46621 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    46681 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    46741 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    46801 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    46861 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    46921 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    46981 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    47041 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    47101 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    47161 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    47221 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    47281 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    47341 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    47401 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    47461 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    47521 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    47581 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    47641 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    47701 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
    47761 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    47821 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    47881 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    47941 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    48001 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    48061 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    48121 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    48181 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    48241 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    48301 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    48361 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    48421 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    48481 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    48541 cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    48601 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    48661 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    48721 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    48781 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    48841 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    48901 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    48961 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    49021 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    49081 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    49141 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    49201 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    49261 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    49321 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    49381 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    49441 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    49501 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    49561 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    49621 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    49681 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    49741 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    49801 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    49861 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    49921 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    49981 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    50041 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    50101 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    50161 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    50221 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    50281 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    50341 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    50401 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    50461 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    50521 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    50581 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    50641 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    50701 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    50761 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    50821 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    50881 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    50941 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    51001 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    51061 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    51121 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    51181 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    51241 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    51301 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    51361 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    51421 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    51481 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    51541 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    51601 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    51661 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    51721 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    51781 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    51841 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact
    51901 tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa
    51961 gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa
    52021 aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca
    52081 ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct
    52141 ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg
    52201 ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga
    52261 gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg
    52321 atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat
    52381 ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt
    52441 agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat
    52501 gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag
    52561 atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct
    52621 gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc
    52681 tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa
    52741 aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa
    52801 atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt
    52861 tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca
    52921 ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg
    52981 acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg
    53041 ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg
    53101 aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta
    53161 gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct
    53221 ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg
    53281 catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg
    53341 tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca
    53401 cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa
    53461 gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac
    53521 gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg
    53581 gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat
    53641 atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta
    53701 ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa
    53761 aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa
    53821 atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga
    53881 caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga
    53941 tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac
    54001 ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt
    54061 tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc
    54121 tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct
    54181 gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat
    54241 ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg
    54301 cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt
    54361 gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc
    54421 tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca
    54481 aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc
    54541 actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg
    54601 ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct
    54661 attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa
    54721 tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc
    54781 tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct
    54841 gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa
    54901 tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca
    54961 tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact
    55021 gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg
    55081 cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg
    55141 gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag
    55201 caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga
    55261 cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa
    55321 atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat
    55381 gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa
    55441 atatgtacta tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac
    55501 tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg
    55561 ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc
    55621 aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca
    55681 aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac
    55741 tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg
    55801 tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact agtcgctttc
    55861 gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg
    55921 ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg
    55981 cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag
    56041 ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac
    56101 gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc
    56161 ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta
    56221 cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag
    56281 tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc
    56341 caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat
    56401 tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca
    56461 aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct
    56521 tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac
    56581 gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct
    56641 gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca
    56701 actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa
    56761 ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat
    56821 atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga
    56881 ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa
    56941 ataatcatca attgactata aaatcatctg agcaaagata aagacattaa cactactgaa
    57001 aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag
    57061 aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata
    57121 gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc
    57181 ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc
    57241 tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa
    57301 acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga
    57361 tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat
    57421 aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac
    57481 ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt
    57541 atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt
    57601 attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt
    57661 cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc
    57721 agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat
    57781 tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt
    57841 ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg
    57901 ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct
    57961 tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag
    58021 atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag
    58081 caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt
    58141 tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga
    58201 aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    58261 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    58321 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    58381 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    58441 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    58501 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    58561 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    58621 tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca
    58681 taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa
    58741 ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa
    58801 caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag
    58861 aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa
    58921 cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag
    58981 caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc
    59041 tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa
    59101 ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa
    59161 aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga
    59221 ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga
    59281 ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt
    59341 tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt
    59401 ttatcataag caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag
    59461 acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt
    59521 attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt
    59581 tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta
    59641 atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt
    59701 tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga
    59761 cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat
    59821 ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct
    59881 tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta
    59941 ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt gattacagga
    60001 aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag
    60061 gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc
    60121 ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta accagctgca
    60181 tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct
    60241 gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca
    60301 aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt
    60361 ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt
    60421 ccactatagc gagtgataac gacactgttg attaataagc cataaccacg taattcatcg
    60481 attaaccgca agatatccat atcataagta atcccataat caccacggat cttattacgt
    60541 tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc
    60601 aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca
    60661 actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata
    60721 tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc
    60781 cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga
    60841 aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg
    60901 gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc
    60961 atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga
    61021 attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca
    61081 tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc
    61141 atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac
    61201 caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt
    61261 acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa
    61321 gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga
    61381 atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa
    61441 gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag
    61501 aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa
    61561 gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt
    61621 tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca
    61681 aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa
    61741 tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg
    61801 actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg
    61861 aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata
    61921 aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc
    61981 aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc
    62041 agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg
    62101 tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag
    62161 caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg
    62221 aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa
    62281 acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat
    62341 gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact
    62401 ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg
    62461 tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa
    62521 gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta
    62581 ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca
    62641 agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa
    62701 cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg
    62761 aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa
    62821 gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat
    62881 gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag
    62941 atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt
    63001 accgttaatc agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa
    63061 tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc
    63121 tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct
    63181 ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac
    63241 ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga
    63301 aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga
    63361 cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt
    63421 gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag
    63481 cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat
    63541 ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg
    63601 ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg
    63661 gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg
    63721 agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg
    63781 gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg
    63841 atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc
    63901 tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa
    63961 aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag
    64021 tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc
    64081 aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa
    64141 aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt
    64201 catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac
    64261 tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc
    64321 ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt
    64381 gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa
    64441 aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa
    64501 atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat
    64561 cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca
    64621 gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc
    64681 aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac
    64741 gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt
    64801 ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga
    64861 aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt
    64921 tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac
    64981 ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct
    65041 gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt
    65101 gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct
    65161 gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga
    65221 tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat
    65281 gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc
    65341 aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata
    65401 atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg
    65461 tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct
    65521 tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag gattagcttg
    65581 cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct
    65641 ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt
    65701 tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag
    65761 tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact
    65821 gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa
    65881 aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat
    65941 cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac
    66001 ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg
    66061 tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt
    66121 gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa
    66181 agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt
    66241 cccggaacag gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc
    66301 tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag
    66361 tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag
    66421 cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag
    66481 ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt
    66541 tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa
    66601 aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc
    66661 aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa
    66721 gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat
    66781 agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga
    66841 tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct
    66901 atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat
    66961 tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc
    67021 caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc
    67081 ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac
    67141 tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg
    67201 ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa
    67261 tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg
    67321 aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg
    67381 acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag
    67441 ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt
    67501 ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc
    67561 gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc
    67621 aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg
    67681 aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca
    67741 tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc
    67801 ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc
    67861 cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg
    67921 atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat
    67981 tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa
    68041 ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg
    68101 gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc
    68161 actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa
    68221 aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga
    68281 atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa
    68341 gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga
    68401 aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca
    68461 cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt
    68521 tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg
    68581 aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta
    68641 tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt
    68701 ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag
    68761 attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga
    68821 aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg
    68881 tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact
    68941 ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg
    69001 aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg
    69061 cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg
    69121 cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa
    69181 ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta
    69241 ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata
    69301 atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa
    69361 tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca
    69421 ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat
    69481 catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat
    69541 ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat
    69601 atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg
    69661 agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga
    69721 ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg
    69781 ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac
    69841 tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc
    69901 gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta
    69961 tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga
    70021 agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca
    70081 aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct
    70141 aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta
    70201 acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc
    70261 catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat
    70321 cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa
    70381 ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca
    70441 gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa
    70501 caagaccaag aattactaga agctatttac caaacagata cacttgaagc tgtactgaca
    70561 ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta
    70621 gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa
    70681 atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga
    70741 gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta
    70801 ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat
    70861 taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa
    70921 atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa
    70981 aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa
    71041 gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat
    71101 aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact
    71161 gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa
    71221 cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga
    71281 agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt
    71341 ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa
    71401 caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc
    71461 gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct
    71521 gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga
    71581 caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc
    71641 caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca
    71701 cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga
    71761 aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt
    71821 gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa
    71881 tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt
    71941 gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt
    72001 tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg
    72061 ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt
    72121 cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt
    72181 agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc
    72241 cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc
    72301 caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac
    72361 ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa
    72421 gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag
    72481 gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata
    72541 tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt
    72601 tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt
    72661 atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg
    72721 tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg
    72781 aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc
    72841 ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag
    72901 aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata
    72961 attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt
    73021 atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag
    73081 aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag
    73141 atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct
    73201 caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg
    73261 aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac
    73321 aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct
    73381 ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt
    73441 ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac catgatgttc
    73501 ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag
    73561 caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa
    73621 tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa
    73681 gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc
    73741 gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg
    73801 gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag
    73861 aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta
    73921 gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac
    73981 aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa
    74041 cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct
    74101 tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc
    74161 ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga
    74221 ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg
    74281 atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga
    74341 ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg
    74401 ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca
    74461 gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt
    74521 tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg
    74581 acagctagcg caatttcttt aggatccaac aactctttta acgtacggat gatccattga
    74641 ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat
    74701 cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt
    74761 tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt
    74821 caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag
    74881 tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca
    74941 atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa
    75001 aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg
    75061 taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat
    75121 caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc
    75181 ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa
    75241 aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt
    75301 agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat
    75361 cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg
    75421 ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac
    75481 cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa
    75541 ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa
    75601 tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag
    75661 agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa
    75721 caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag
    75781 tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga
    75841 agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta
    75901 atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt
    75961 ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga
    76021 atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat
    76081 cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa
    76141 cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa
    76201 aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt
    76261 gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga
    76321 ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc
    76381 accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat
    76441 gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt
    76501 cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa
    76561 ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa
    76621 cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta
    76681 attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag
    76741 cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct
    76801 ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa
    76861 ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag
    76921 catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag
    76981 taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt
    77041 ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca
    77101 tggacatcaa agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag
    77161 cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc
    77221 ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg
    77281 tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt
    77341 ccattaaata aagaatag
//
LOCUS       DACERL010000003        74190 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.003, whole genome shotgun sequence.
ACCESSION   DACERL010000003 DACERL010000000
VERSION     DACERL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74190
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.003"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(176..1462)
                     /locus_tag="IXF54_000178"
     CDS             complement(176..1462)
                     /locus_tag="IXF54_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1403464.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IXF54_000179"
     CDS             complement(1455..2723)
                     /locus_tag="IXF54_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1403465.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IXF54_000180"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IXF54_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1403466.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IXF54_000181"
     CDS             complement(3495..3941)
                     /locus_tag="IXF54_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403467.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IXF54_000182"
     CDS             complement(4034..4279)
                     /locus_tag="IXF54_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403468.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IXF54_000183"
     CDS             complement(4394..4972)
                     /locus_tag="IXF54_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1403469.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IXF54_000184"
     CDS             complement(5049..5579)
                     /locus_tag="IXF54_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1403470.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IXF54_000185"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IXF54_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1403471.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IXF54_000186"
     CDS             complement(6436..6831)
                     /locus_tag="IXF54_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1403472.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IXF54_000187"
     CDS             complement(6828..8240)
                     /locus_tag="IXF54_000187"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1403473.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IXF54_000188"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IXF54_000188"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1403474.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IXF54_000189"
     CDS             complement(9095..10555)
                     /locus_tag="IXF54_000189"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1403475.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IXF54_000190"
     CDS             complement(10725..11882)
                     /locus_tag="IXF54_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1403476.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IXF54_000191"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IXF54_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1403477.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IXF54_000192"
     CDS             complement(13349..13876)
                     /locus_tag="IXF54_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1403478.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="IXF54_000193"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="IXF54_000193"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1403479.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="IXF54_000194"
     CDS             14909..16156
                     /locus_tag="IXF54_000194"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1403480.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="IXF54_000195"
     CDS             16232..16576
                     /locus_tag="IXF54_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403481.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="IXF54_000196"
     CDS             complement(16693..17019)
                     /locus_tag="IXF54_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403482.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="IXF54_000197"
     CDS             complement(17634..18836)
                     /locus_tag="IXF54_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1403483.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="IXF54_000198"
     CDS             18953..19468
                     /locus_tag="IXF54_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1403484.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="IXF54_000199"
     CDS             complement(19667..20113)
                     /locus_tag="IXF54_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1403485.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr"
                     /locus_tag="IXF54_000200"
     CDS             complement(20160..21638)
                     /gene="msr"
                     /locus_tag="IXF54_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1403486.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="IXF54_000201"
     CDS             complement(21992..22621)
                     /locus_tag="IXF54_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1403487.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="IXF54_000202"
     CDS             complement(22605..23069)
                     /locus_tag="IXF54_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1403488.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="IXF54_000203"
     CDS             complement(23556..24824)
                     /locus_tag="IXF54_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1403489.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="IXF54_000204"
     CDS             complement(25055..25792)
                     /locus_tag="IXF54_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1403490.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="IXF54_000205"
     CDS             complement(25859..26428)
                     /locus_tag="IXF54_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1403491.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="IXF54_000206"
     CDS             26556..27428
                     /locus_tag="IXF54_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1403492.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27770..28642
                     /locus_tag="IXF54_000207"
     CDS             27770..28642
                     /locus_tag="IXF54_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1403493.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28639..30597
                     /locus_tag="IXF54_000208"
     CDS             28639..30597
                     /locus_tag="IXF54_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1403494.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30929..31780)
                     /locus_tag="IXF54_000209"
     CDS             complement(30929..31780)
                     /locus_tag="IXF54_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1403495.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31903..32370
                     /locus_tag="IXF54_000210"
     CDS             31903..32370
                     /locus_tag="IXF54_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1403496.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32633..33115)
                     /locus_tag="IXF54_000211"
     CDS             complement(32633..33115)
                     /locus_tag="IXF54_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1403497.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33215..34078)
                     /locus_tag="IXF54_000212"
     CDS             complement(33215..34078)
                     /locus_tag="IXF54_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1403498.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            34676..35551
                     /locus_tag="IXF54_000213"
     CDS             34676..35551
                     /locus_tag="IXF54_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403499.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(35696..35848)
                     /locus_tag="IXF54_000214"
                     /pseudo
     CDS             complement(35696..35848)
                     /locus_tag="IXF54_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(36116..37447)
                     /locus_tag="IXF54_000215"
     CDS             complement(36116..37447)
                     /locus_tag="IXF54_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1403500.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(37596..38309)
                     /locus_tag="IXF54_000216"
     CDS             complement(37596..38309)
                     /locus_tag="IXF54_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1403501.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            38484..39908
                     /locus_tag="IXF54_000217"
     CDS             38484..39908
                     /locus_tag="IXF54_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1403502.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            40207..40914
                     /locus_tag="IXF54_000218"
     CDS             40207..40914
                     /locus_tag="IXF54_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1403503.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            40901..41224
                     /locus_tag="IXF54_000219"
     CDS             40901..41224
                     /locus_tag="IXF54_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1403504.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(41313..42350)
                     /locus_tag="IXF54_000220"
     CDS             complement(41313..42350)
                     /locus_tag="IXF54_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1403505.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(42671..43654)
                     /locus_tag="IXF54_000221"
     CDS             complement(42671..43654)
                     /locus_tag="IXF54_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1403506.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(44069..44839)
                     /locus_tag="IXF54_000222"
     CDS             complement(44069..44839)
                     /locus_tag="IXF54_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403507.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(44832..45767)
                     /locus_tag="IXF54_000223"
     CDS             complement(44832..45767)
                     /locus_tag="IXF54_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403508.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(46083..47732)
                     /locus_tag="IXF54_000224"
     CDS             complement(46083..47732)
                     /locus_tag="IXF54_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1403509.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(47849..50452)
                     /locus_tag="IXF54_000225"
     CDS             complement(47849..50452)
                     /locus_tag="IXF54_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1403510.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(50464..51513)
                     /locus_tag="IXF54_000226"
     CDS             complement(50464..51513)
                     /locus_tag="IXF54_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ1403511.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(51539..51853)
                     /locus_tag="IXF54_000227"
     CDS             complement(51539..51853)
                     /locus_tag="IXF54_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ1403512.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(51869..52315)
                     /locus_tag="IXF54_000228"
     CDS             complement(51869..52315)
                     /locus_tag="IXF54_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1403513.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(52316..54292)
                     /locus_tag="IXF54_000229"
     CDS             complement(52316..54292)
                     /locus_tag="IXF54_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ1403514.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(54450..55346)
                     /locus_tag="IXF54_000230"
     CDS             complement(54450..55346)
                     /locus_tag="IXF54_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1403515.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(55339..56019)
                     /locus_tag="IXF54_000231"
     CDS             complement(55339..56019)
                     /locus_tag="IXF54_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1403516.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(56020..56802)
                     /locus_tag="IXF54_000232"
     CDS             complement(56020..56802)
                     /locus_tag="IXF54_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1403517.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(56795..57712)
                     /locus_tag="IXF54_000233"
     CDS             complement(56795..57712)
                     /locus_tag="IXF54_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403518.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(57876..58199)
                     /locus_tag="IXF54_000234"
     CDS             complement(57876..58199)
                     /locus_tag="IXF54_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1403519.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            58987..62214
                     /gene="fss3"
                     /locus_tag="IXF54_000235"
     CDS             58987..62214
                     /gene="fss3"
                     /locus_tag="IXF54_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1403520.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            62327..62641
                     /locus_tag="IXF54_000236"
     CDS             62327..62641
                     /locus_tag="IXF54_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1403521.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            62654..63028
                     /locus_tag="IXF54_000237"
     CDS             62654..63028
                     /locus_tag="IXF54_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1403522.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            63038..63373
                     /locus_tag="IXF54_000238"
     CDS             63038..63373
                     /locus_tag="IXF54_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1403523.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            63456..64796
                     /locus_tag="IXF54_000239"
     CDS             63456..64796
                     /locus_tag="IXF54_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1403524.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            64874..65548
                     /locus_tag="IXF54_000240"
     CDS             64874..65548
                     /locus_tag="IXF54_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1403525.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            65781..66215
                     /gene="dcm"
                     /locus_tag="IXF54_000241"
     CDS             65781..66215
                     /gene="dcm"
                     /locus_tag="IXF54_000241"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1403526.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            66216..66923
                     /locus_tag="IXF54_000242"
     CDS             66216..66923
                     /locus_tag="IXF54_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1403527.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            66913..67203
                     /locus_tag="IXF54_000243"
     CDS             66913..67203
                     /locus_tag="IXF54_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403528.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            67462..68646
                     /locus_tag="IXF54_000244"
     CDS             67462..68646
                     /locus_tag="IXF54_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1403529.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            68661..68780
                     /locus_tag="IXF54_000245"
     CDS             68661..68780
                     /locus_tag="IXF54_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1403530.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            69526..71436
                     /gene="ltrA"
                     /locus_tag="IXF54_000246"
     CDS             69526..71436
                     /gene="ltrA"
                     /locus_tag="IXF54_000246"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1403531.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            71540..71764
                     /locus_tag="IXF54_000247"
     CDS             71540..71764
                     /locus_tag="IXF54_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403532.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            71777..72280
                     /locus_tag="IXF54_000248"
     CDS             71777..72280
                     /locus_tag="IXF54_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1403533.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            72340..72729
                     /locus_tag="IXF54_000249"
     CDS             72340..72729
                     /locus_tag="IXF54_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1403534.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            72716..>74190
                     /locus_tag="IXF54_000250"
     CDS             72716..>74190
                     /locus_tag="IXF54_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1403535.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        22396 a        14736 c        13633 g        23425 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    27541 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    27601 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    27661 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    27721 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    27781 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    27841 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    27901 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    27961 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    28021 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    28081 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    28141 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    28201 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    28261 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    28321 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    28381 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    28441 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    28501 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    28561 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    28621 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    28681 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    28741 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    28801 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    28861 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    28921 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    28981 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    29041 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    29101 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    29161 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    29221 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    29281 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    29341 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    29401 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    29461 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    29521 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    29581 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    29641 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    29701 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    29761 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    29821 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    29881 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    29941 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    30001 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    30061 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    30121 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    30181 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    30241 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    30301 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    30361 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    30421 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    30481 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    30541 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    30601 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    30661 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    30721 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    30781 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    30841 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    30901 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    30961 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    31021 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    31081 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    31141 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    31201 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    31261 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    31321 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    31381 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    31441 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    31501 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    31561 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    31621 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    31681 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    31741 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    31801 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    31861 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    31921 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    31981 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    32041 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    32101 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    32161 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    32221 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    32281 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    32341 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    32401 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    32461 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    32521 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    32581 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    32641 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    32701 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    32761 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    32821 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    32881 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    32941 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    33001 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    33061 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    33121 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    33181 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    33241 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    33301 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    33361 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    33421 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    33481 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    33541 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    33601 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    33661 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    33721 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    33781 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    33841 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    33901 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    33961 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    34021 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    34081 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    34141 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    34201 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    34261 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    34321 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    34381 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    34441 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    34501 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    34561 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    34621 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    34681 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    34741 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    34801 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    34861 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    34921 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    34981 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcaatttat
    35041 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    35101 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    35161 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgctcattc agcaaaatgc
    35221 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    35281 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    35341 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    35401 agtcgatcat actgacgcct cttattcaaa acgctactcc ataaaaatcc tgaaagaaga
    35461 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaaa ctaaagaaac
    35521 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    35581 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    35641 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    35701 agtcatgaca aatttccttc gttgctgggt agattttccg gcattgttga tagattttat
    35761 gatacttttc tacattttgt aagagaggtt ccactgcatc tgtatattgg ataaaattct
    35821 gacaacaact ttcaggtgtg tcgaaccact ctaatcttta aagataaata ttaaaataac
    35881 gattaataat attaacagtc tttatttttc accaccaaaa acctatcttt gcatatacta
    35941 aatagacgta tatacacaga taggtttgtc ttgtgaaaaa taatgattat ccttaccaaa
    36001 aaatgtttgt ttttctacat gttcgtactt atcaaaaaat atgatcctta ttgaaattac
    36061 tggctattta tattccaacg acttcttgtg ttaatttttg ttatctcata gtaaattatg
    36121 tttcattttc acttaattta gttgtcacgt gttcgtcaat tcgtaaaaac ggtaaatata
    36181 taaacacact tgcggctata attaatattt gtaaaacagc tgctcgccag tctccggctg
    36241 ttgctaaaaa tcccgatata actggtggcg tagtccaagg aatttgcact actacatgat
    36301 taattaatcc cacagcagtt gcaaaatatg ctactgtcaa agaaaaaatc ggtgcaagca
    36361 caaagggaat aatcagactg atattaaata caataggcaa tccaaaaata ataggttcgc
    36421 ttatattgaa tatagcggga gttaccgata atttagcaaa gtccttataa tcttttctac
    36481 gactgaagat aaatattgca atcaataaag caatcgtatt tcccgatcca cccattaccg
    36541 caaaggtatc tttaaaggcc atattgataa tatgtggaat ttgctctttg tttgcataag
    36601 ctgccatatt ttcttgcata ttctgaatca aaaacggatc taacaatgaa cctgatatta
    36661 ctgcttgatg gataccaaat ccaaaaaata gattagcaat agatgtaatt aacaaaaatc
    36721 ctggtaaacc agttgtaatt ttacttaatg gctctgtaac tatcgtggta attagcttat
    36781 ttatatccat attaaacaat tgaatgatta taaatgacaa taaagcaaac gaggtaatcg
    36841 taataatgat tgggattaat acattaaaag actttaaaac agcgggagga atatttccgg
    36901 atagctgaat ctgtagtttc ttattattag aaagtttaat aaataattct gtagccgcta
    36961 atccaacaaa tatacctaca aacattcctg aagctcctgt ataggatata ggtatcacac
    37021 ccgtcacttc aacagcttta tccgcatttt caggagtaaa catcatctgc ataggtgtta
    37081 atacaacaaa tgtcgaaata gcaactaata ccgctgcaat atgatttgta taatttctgc
    37141 ttgcacacat atgataagat actgctgcta caactaataa tgtagttatg cttaatgtac
    37201 cattggcaat tgaagtacca atttgttgta acgtggtcaa tgtactagta tcgacaattt
    37261 cccccatgaa tcctgttggt tctaagatca ctgaattaat aagtgtcaca aatcctgcta
    37321 acatcataaa tggcataaat gtagcaaacg catctcgcaa agttcttaaa tggatctgat
    37381 tccccaactt agtcgcaact ataccaaatt tttcaaatac aatgtccttc ctgctacttt
    37441 tgcccatatt ttttctcctt tatgcttatt gtaatcgatt actttttcca cccaaataat
    37501 acatcactta tcaaaaaaag taaatacttt taattaaata tatatatctt ttttagtata
    37561 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
    37621 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
    37681 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
    37741 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
    37801 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
    37861 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
    37921 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
    37981 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
    38041 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
    38101 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
    38161 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
    38221 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
    38281 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
    38341 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
    38401 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
    38461 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
    38521 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
    38581 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
    38641 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
    38701 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
    38761 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
    38821 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
    38881 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
    38941 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
    39001 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
    39061 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
    39121 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
    39181 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
    39241 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
    39301 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
    39361 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
    39421 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
    39481 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
    39541 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
    39601 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
    39661 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
    39721 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
    39781 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
    39841 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
    39901 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
    39961 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
    40021 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
    40081 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
    40141 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
    40201 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
    40261 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
    40321 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
    40381 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
    40441 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
    40501 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
    40561 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
    40621 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
    40681 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
    40741 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
    40801 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
    40861 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
    40921 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
    40981 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
    41041 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
    41101 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
    41161 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
    41221 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
    41281 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
    41341 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
    41401 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
    41461 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
    41521 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
    41581 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
    41641 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
    41701 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
    41761 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
    41821 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
    41881 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
    41941 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
    42001 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
    42061 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
    42121 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
    42181 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
    42241 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
    42301 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
    42361 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
    42421 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
    42481 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
    42541 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
    42601 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
    42661 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
    42721 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    42781 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    42841 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    42901 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
    42961 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
    43021 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
    43081 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    43141 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    43201 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
    43261 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
    43321 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    43381 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    43441 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
    43501 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    43561 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    43621 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    43681 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    43741 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
    43801 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
    43861 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    43921 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    43981 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    44041 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    44101 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
    44161 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accgaagatc atataaacga
    44221 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
    44281 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
    44341 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
    44401 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    44461 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
    44521 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
    44581 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
    44641 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
    44701 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
    44761 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    44821 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
    44881 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    44941 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    45001 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
    45061 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
    45121 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
    45181 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
    45241 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
    45301 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    45361 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    45421 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    45481 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
    45541 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
    45601 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
    45661 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
    45721 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    45781 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
    45841 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
    45901 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
    45961 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    46021 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
    46081 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
    46141 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
    46201 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
    46261 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
    46321 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
    46381 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
    46441 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
    46501 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
    46561 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
    46621 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
    46681 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
    46741 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
    46801 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
    46861 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
    46921 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
    46981 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
    47041 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
    47101 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
    47161 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
    47221 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
    47281 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
    47341 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
    47401 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
    47461 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
    47521 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    47581 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    47641 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    47701 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    47761 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    47821 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    47881 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    47941 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    48001 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    48061 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    48121 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    48181 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    48241 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    48301 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    48361 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    48421 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    48481 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    48541 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    48601 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    48661 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    48721 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    48781 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    48841 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    48901 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    48961 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    49021 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    49081 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    49141 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    49201 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    49261 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    49321 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    49381 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    49441 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    49501 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    49561 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    49621 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    49681 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    49741 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    49801 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    49861 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    49921 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    49981 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    50041 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    50101 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    50161 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    50221 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    50281 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    50341 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    50401 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    50461 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    50521 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    50581 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    50641 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    50701 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    50761 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    50821 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    50881 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    50941 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    51001 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    51061 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    51121 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    51181 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    51241 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    51301 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    51361 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    51421 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    51481 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    51541 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    51601 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    51661 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    51721 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    51781 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    51841 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    51901 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    51961 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    52021 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    52081 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    52141 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    52201 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    52261 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    52321 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    52381 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    52441 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    52501 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    52561 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    52621 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    52681 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    52741 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    52801 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    52861 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    52921 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    52981 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    53041 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    53101 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    53161 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    53221 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
    53281 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
    53341 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
    53401 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    53461 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    53521 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    53581 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    53641 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    53701 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    53761 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    53821 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    53881 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    53941 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    54001 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    54061 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    54121 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    54181 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    54241 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    54301 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag
    54361 cctgcaatat ttatatcaca ggcttttttc tctattaaat atatataaga taatacattt
    54421 ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag
    54481 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
    54541 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
    54601 aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact
    54661 gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa
    54721 cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat
    54781 acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt
    54841 tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc
    54901 atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt
    54961 aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct
    55021 acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga
    55081 gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt
    55141 tgttcatttt catccaacca tgtattcagt gcatctacta ggtccattag attcttttct
    55201 cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc
    55261 ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca
    55321 acgagaagca acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata
    55381 cacagcatgt gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt
    55441 ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac
    55501 ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac
    55561 agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata
    55621 gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat
    55681 tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc
    55741 caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt
    55801 cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc
    55861 tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata
    55921 agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc
    55981 atcgtcttcg ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg
    56041 gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat
    56101 tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc
    56161 cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa
    56221 ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat
    56281 gaatgtcaaa gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa
    56341 tacttttaga acataatttc catagttttt gattccctca acttcaccaa atccgaaagc
    56401 aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa
    56461 aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt
    56521 attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa
    56581 agccagaaga atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat
    56641 gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt
    56701 ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct
    56761 cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta
    56821 attgtaagaa ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt
    56881 ttgcttctac caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga
    56941 taacacggct agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg
    57001 cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt
    57061 ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct
    57121 gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca
    57181 aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt
    57241 catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc
    57301 gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt
    57361 tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt
    57421 tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc
    57481 gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga
    57541 tcaaacccat aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc
    57601 catagatcat tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt
    57661 attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc
    57721 ttctttctta ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt
    57781 ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc
    57841 tttcctatta tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg
    57901 accaaagtcg tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc
    57961 ttgagtaatt ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa
    58021 attagactgt ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt
    58081 gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct
    58141 tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt
    58201 aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta
    58261 ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt
    58321 ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg
    58381 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa
    58441 taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat
    58501 agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg
    58561 tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta
    58621 cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa
    58681 gctttacggg ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa
    58741 ccttgattta taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat
    58801 gtttgtgttc taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat
    58861 ttttaggctc attcattctc aaatccattc tattcagtca ataaaaattt taaaaagact
    58921 ttataaacaa agatgttcaa gcgagcatct tatttttttg tgcttaattt gaaaggagaa
    58981 catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct
    59041 gttcaagcaa ttgttggagc aattgctcct acgattactt ttgctgatga aattacccat
    59101 ccacaaacgg taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt
    59161 agtgatggtc gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcttt
    59221 aaacaagata ttttctgtat tgaaccagga gtcccgattt acaatgagtt cactcctggc
    59281 tatgagaaaa acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg
    59341 aaaaaagtag gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa
    59401 gttaatgggt atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca
    59461 gccattgagg caaaaataaa tcaagccatt gacgattatc agaagaaacc aaccttccat
    59521 aatagtacgg ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg
    59581 aatttgtcag agttcgatga ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat
    59641 ggtaatcagt tgataatcac cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt
    59701 aagaaatctg ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta
    59761 atggccgggg cgattgataa acctaatacc tacacaatca aaattgatgt ggaaacggag
    59821 ggttctttaa agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc aggaacggtt
    59881 ttccatttag actttggaaa aactttacct gcaaaagacg tgactactga caaagaaggc
    59941 attgctacat tggatgagat tcctcatggt acaaaagtaa ccattaccga aaaatcagta
    60001 ccggcgcctt atacgattga taccaccccc atgactacca ccattaaagc aggcgaaacc
    60061 atttacgtga cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga taaaagcgga
    60121 gttgaaacag ggagtgatct ttggaatgac aattattcct tagctggtaa tacgtttgcc
    60181 attcgcaaag acagtccaac cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt
    60241 catgcggaaa caccaaaaga gattgctaat gcgttggaat tgggaaccta ttatgtgacc
    60301 gaaactaaag ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag
    60361 tatgccaatc aaaccgtagc ccctgttacc agtaacgtaa aagggcaaaa tcaagaagtc
    60421 actggcgaaa ccactttgac gaaagaagac aaagatactg gggataaagc acaaggtaag
    60481 gcagtctttg agggtgctga atacaccttg tttactgcta aggatggaaa agctgttaaa
    60541 tggagtgagg cttttaaacc agagttagcg aagggaacga aaggttctga tgaaacggtc
    60601 actttgactt tagacgaaaa gaaccaagtt gccgttaaac atttagctat caacgagtat
    60661 tattgccaag aaacaaaagc gcccgaagga tatagccttg atgaaacaaa gtatcctgta
    60721 tccatcaaaa aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca
    60781 aaagaacaag tgattcgctt tggctttgat ttctttaaat ttgctggatc agctgcgggc
    60841 actaccgaaa ctggcttcaa tgatctgacc tttaaagtgt cgccattgga agggaccaat
    60901 gaaatcacag gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat
    60961 ggttatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc
    61021 ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa
    61081 gaagactttg tgaagagtga gtatgtcttt accattaccg aacaagacca aaaacaacca
    61141 attaaaacgg tgaccgttcc ttacgagaag ctgacgaaca aagcattttc tgttagtttg
    61201 aaccgtttaa tgctctatga tttgcctgag gaagaagata gtttaacttc tcttgcgact
    61261 tggaaagacg gaaacaaaga attaacttct ctggattcta ccgagctagt tgataaattg
    61321 agttacaatc tacatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg
    61381 gatgcgacaa aagctgccca agaaaaagat gaaaaagcca aaccagtggt gattgcagaa
    61441 acaagcgcaa cgttggcgaa caaagagaaa acaggaactt ggaaaattca gcataaatta
    61501 accgccgaac aagttttaaa taaaactatt gtcttattca actatgtgta tgaaaataag
    61561 gaagcttttg aagcaggcga taagccagta gcgaaggatg ttagcttgaa taaccaagcc
    61621 caaactgtca gttgtacagt ggaacaccat gtttctatcc aaacaaaggc ccacctagaa
    61681 aacggttctc agacctttac tcatggtgat gtggtggata tgtttgatga tgtgtcgatt
    61741 actcatgatg tactggatgg ctcaaaagaa gctttcgaaa caattctgta tgctttatta
    61801 ccggatggta caaacaaaga aatttggaaa tctggcaaga ttgattatga agtaaatgat
    61861 aaagaattca ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata tccagaagga
    61921 accaagttta cttttgcgga aatcaattac gataaagatg ggaatatcaa tggaaaacac
    61981 aatgaagatt tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc aaccatacta
    62041 agtacgccga aacaaccgga aacaccaact gttccaagtg gctctcaaga atctagtccc
    62101 acagtgaaga ccttccctca aaccggggag aaaaattcca atgttttact gttcattggt
    62161 tttactttga tttttgcgac ggcgggctat tatttctgga atcgccggaa ctaaggtgat
    62221 gagatgatac aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc
    62281 gcgctatttt tgtatccaaa actaacaatg aaaagaggaa aagaagatgg aattgaaatt
    62341 tgtcgtgcca gatatggctg aaacctttgg aaagttaagt tatgccggtg aaggagaaat
    62401 tataacagaa ggatatggac ggaatactac ggtgattggt cgcagttatc atttgtattc
    62461 cagtaagcaa cgggccgatg atattgaggt ggtagtagct gcggaagccg gtgaaaagga
    62521 ttttgatcaa gaccaaccgc taaaagccgt gaatccccat ttggttgcca aaggctatga
    62581 gattgagaat cgtgggttta ctgattacgt gttgtatgtt gatgatttag tgaaagcata
    62641 ggaggaaaag aagatgagat taacagaagg tattgtcgta gattcaggtt taacatttgg
    62701 gaagttgcgt ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacaat
    62761 tggtaatgag gtaaaagaac gaacctataa tttaaaatcc tctgcacaag ggcgaatgat
    62821 tcaagttagt atcccagcta atgtaccctt acgcgagttt gcttatgatg cagaggtaga
    62881 gttggtcaat ccaatcgtgg ataccgttgc caattatgtt tttcgagaag gaactaccgt
    62941 taattggttc attaaggcgg atgatttagt cttgaagcgg cagccgaatc aaggaaatcc
    63001 tacgaaccaa aatgaaagaa agaaataggt gaagattatg gaaattaatg ttgaacatat
    63061 tttagattgt ctcgatcaat atggaaaagg cgagctgact gaggaacaac tcgttaaagc
    63121 attaacctct gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg gagaaaatag
    63181 cgacagtgaa gaagactctg atgtactaga ctggttagga gaagaagagt ctttctttat
    63241 agttgtcgaa gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg aagaaattgg
    63301 tgtggagatg ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact
    63361 cccctcctcg tagcagtaaa tgactagcat aactttttga aattgcctaa tgaaatccta
    63421 ttgggctttt tcgattggag gtggaaacca tcaaaatgta caaaggacat cgcatacgcg
    63481 caggagatca gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg
    63541 gttggatgag cgtcttttat tttcaagagt ttagacaatt tgatatttct aagctatcac
    63601 tttctactat tgaaatcgtt tggtctataa aagatttagt atgtctgctt ggaagtctcg
    63661 ctttttcagg agctatgata ttgctttata ttcacttttt tcttgatcat tggcgaagct
    63721 tgtggcatcg acaaaagcta gctcggatga ttctagagaa ccattggtat gaagtgaaac
    63781 aaactcaaag tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga gagactatca
    63841 gttacttccc caaaatctgt tatcgaatga aagatggatt actgtctatt cgcgttcaaa
    63901 tttcacttgg gaaataccaa gatcagcttt taaaattgga aaagaagtta gaaagcggac
    63961 tgtattgtga gttagtagaa aaggaactca aagactccta tgtggagtat actttactgt
    64021 atgatatgat cgccaatcga attgggatag acgaagtagt ggcagaaaat gggactctgc
    64081 gattgatgaa aaatcaagta tgggcttatg attctttacc ccatatgtta atcgctggtg
    64141 gcacgggtgg agggaagacc tatttcctcc tcaccattat cgaagcacta ttgaagtcag
    64201 atgcagaatt gtttatcctc gatcccaaaa atgcagattt agctgattta ggtacggtga
    64261 tgcctcatgt ttattcacaa aaggaagaaa tttctgcttg tgtggaagat ttctatgaac
    64321 gtatgatggc tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt
    64381 atgcctatct tggacttcca cccaactttt taatctttga tgaatacgtg gcttatatgg
    64441 agatgttaac gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt
    64501 tgggtcgtca atctggtttc tttctgattt tggcttgcca aaggccggat gcgaaatatt
    64561 taggagacgg aatccgcgat caattcaact ttcgcgtggc tttgggtcgt atgagtgaac
    64621 tcggatattc catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag
    64681 gtcgaggtta tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac
    64741 caaaagggta tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga
    64801 tcttcaatac tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa
    64861 ggggataatt aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga
    64921 taagtttatt tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt
    64981 tatggcaggg agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta
    65041 tgataaccat gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg
    65101 aaaaaactct agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata
    65161 tgtcttagat catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga
    65221 aaaaaatatc tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca
    65281 agaccctaaa atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt
    65341 gccgaaaaag gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa
    65401 gagtaaacat ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc
    65461 cgaagttcat tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa
    65521 agatttggag gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat
    65581 acaataagag cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga
    65641 ttaaatatgt agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa
    65701 aatagtagga caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt
    65761 tgcaatgaat gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc
    65821 gtttaggtat ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg
    65881 ctagacgtag ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca
    65941 caagtgtatc agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat
    66001 ttccgtgcca agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta
    66061 ctttattctt tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg
    66121 agaacgtccg aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa
    66181 cgctggatgg attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc
    66241 cccaaagccg ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag
    66301 tatttcctat cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata
    66361 tcaatccttc caatcgagga atgggtggac aagtctatgg ttcagatggc gtagcgccaa
    66421 ccttaacagg tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag
    66481 caggactatt accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg
    66541 cccccacctt gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag
    66601 aatatcaaga ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc
    66661 gccgaggtcg tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga
    66721 tcgtcacgga tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg
    66781 gctttcccga ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata
    66841 agcaagccgg aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag
    66901 agttggatgt acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag
    66961 attcagtcag acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag
    67021 aatggtgtca aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatt
    67081 gaaataaatc aagtaattgc agaatttgag cggattgcgg accaagcaaa agaaccagat
    67141 attcgtcaat acttgttaga aatgaaagca gagttggaag taaatggtat tacagatgag
    67201 taacctaaac caacacaact gactgttagg cgctgaccgc ggaggattcg cgaagcagaa
    67261 atcctccgtg gtgctgtgcc tgttcatgga cggcgttagc cgaccatgat gtctcacccc
    67321 cgtatctaac aggggggtac aaaaacacaa aaaataagta agcaaccatt aagaatgctt
    67381 gttgtatcaa agatagtaag taatttgtat aggtgttaac tcatttgaaa aagttaacac
    67441 cttgaatttg gaggtgagaa cttggcacaa cggaatttag attatcggct attaaaagac
    67501 cgacgcaacg aatacggcgt ttcacaaaat aaattggcta cggcttgtgg acttagtcgg
    67561 ccgtacttaa atcagataga aaatggtggc gtaaccgcat ctataaaaac catgaggaaa
    67621 atttttgacc aactggaaag ctttaatccc gatttgcctt tgacgttact gtttgactat
    67681 gtaaggattc gctttcccac aacggatgca cgaaaaatta ttcaagaaat tctccatttg
    67741 aaatttgagt atatgctcca tgaagattat gccttttact cctatcaaga acagtatgtc
    67801 atgggtgata ttgtggtgat gttgtctcat gaagaagata aaggtgtgct tttagaatta
    67861 aaggggcgag gttgccgaca atttgaaact tttttactcg ctcaaaagcg tagctggtac
    67921 gactttttcg aggattgtct aaaggccggt ggcgtgatga aacgcttgga cttggcaatc
    67981 aatgatcgag tggggctatt ggatatacct gagctaacga aaaaatgtca gaaggaagaa
    68041 tgtatctcct tgtttcgtac ctttaaaagt tatcgttccg gggaactttt aaaggctgat
    68101 gaaaaggacg gcatgggcaa taccttatat atcggcagtc tcaagagtga ggtttatttc
    68161 tgtttgtatg aaaaagacta tgaacaatat atcaagttag ggattccatt agaccaaact
    68221 gagacgaaaa atcgttttga gattcgactg aaaaatgacc gtgcttttca tgcgattcaa
    68281 gatttgttaa aaggtcgtag cattgagagc accacgtttt ccattatcaa tcgttacttg
    68341 cgatttgcgg ataaagtaga aggtaaaagg cgaaccaact ggcccttgaa tgaacaatgg
    68401 gggcgtttta ttggacggaa tcggaaagag attcaattaa cttccgagcc aaaaccgtat
    68461 accattgaac gaaccttgaa ttggctaggc cgacaagtcg cccctacgtg gaaaatggct
    68521 aaggagttgg atcgattgaa tcggacaacc tatatccagg atatggtaca gaatgcacgc
    68581 ttatcagacc gacataaaaa gattttggag caacaaagta tggcaattga aaatttgatt
    68641 atatgaaaag aggaatcgtc atgggaattg taaaagatat ttttttggta tttggtggca
    68701 gtatgcttgg tgttactgtg atgtgcttga tgaatgcaag tgcagcagcg gatcgagcaa
    68761 tggaaaagaa aggaaagtga caagatgaat ttcggacaaa atttatgtgc gactcgttcc
    68821 ttagtgaaaa gctaaggcac taataaaaga acgaaaaagt ttggttatta ggagaactga
    68881 taatctgaca ggtggaatga tgaagtaacg ctctgaaacg cctgccctga gcctccgaca
    68941 agcattgtat gaggaaactc agaatgtttg aagctcggta aagacggctg aaagataccg
    69001 taatagttga tgaacatata tcttctatcg aaaactatcc agaggtgggt agcgtatcca
    69061 acaggtcggg ggtctataaa atatctatgg ttagaatgtt ctataaggaa ctgacgaaac
    69121 agcgaatgta cgggtctaaa tttgaacttt gtagaaatgc aaaggtacga gaaatcgtag
    69181 ctggtgtggc aaagtaagaa tataggttat gaaatgccat ataacgttac aggcggtatc
    69241 aagccagcag gcttatagct agtgacctaa ggatatatat gtatagataa gattatcgga
    69301 acgaggaaag gtattgggtt ctcatcaaga gaataaactg acgaagaaca ataagcagtt
    69361 gaactaatgc tgaaaagcag agggcgaacc taagaaattc ttgtaatgag aatggaggaa
    69421 tgccctcaag tcgtgtatat attagaaaag tattatgttg tttcaataag gattacgagt
    69481 aagataaaaa agctcactca atttcaagta aaggagtgaa gccttatgcc taaaaataaa
    69541 aatgaaacat tgtgtgtaga agatttaaga cacgctgaat attacgaaat gcaaaacacc
    69601 tttgatgatt tatatgccaa aagtaagaat ggagatattt tcactcatct tatggatatt
    69661 atcctatcga gagaaaatat attactggca tatcgaaata tcaaagcaaa tgcaggcagt
    69721 aaaacagcag gaacagacgg tacaataatt aaagatattg gcaaattgcc agcagaaaca
    69781 gttgtcaaaa aagtaagata cattgttgca ggcagtccac atgggtatcg acctaagcca
    69841 gtgaggcgaa aagaaatacc aaaaccaaat ggtaaaacca gacctctagg tattccttgt
    69901 atgtgggaca ggcttataca acaatgtata aaacaagtat tagaaccaat ctgcgaagct
    69961 aaatttagtg aaaacagcta tggttttaga cctaataggt cagttgaaaa tgcaataaaa
    70021 gcaacctata accgcctaca aatcagccag ctacattatg tgattgaatt tgatattaag
    70081 ggtttctttg acaacgtgaa ccattccaag ctgataaaac agatatgggc tatgggaata
    70141 cgggacaagc acttgatatt cattttaaaa aggatattaa aagctccaat caaaatgact
    70201 aacggtacaa taacatatcc agagaaagga actccgcaag ggggaataat atccccacta
    70261 ctagcaaaca ttgttcttaa tgagttagac cattgggtag aaagccaatg gcaggaaaat
    70321 cctgttacaa aaaactatgt ggttcatatc aataaaagcg gtagcccatg taaaagcaac
    70381 gcttacaaag aaatgaagaa aacaaagtta aaggaaatgt atatggttcg ttatgcagat
    70441 gattttagag ttttctgccg ctacaaggaa agtgctgaaa aagccaaaat agcaatcact
    70501 caatggattg aacaacgatt aaaattggaa gtatcacaag aaaaaacaag aattgtaaat
    70561 gttagaaaaa gatactccga ctttcttggt ttcaagatta aaatgatacc gagaagaaag
    70621 aagttagttg ttaaatcaca tataagtgat aaacaattca aaaaccaaaa agacaagctg
    70681 gtatcacagg caaagaaaat tgcttctccc tcaaaaggga aaacggaatt agaagaaaca
    70741 agactttata attcaatggt attaggtatg caaaattatt atcagattgc aacgaatgta
    70801 aatctggatt gtatgaaact gaatagagct gtaatggcta tcttcactaa cagattaggt
    70861 acaaacaaaa ggggcagatt gagaaaaaca ggcagacaac ttacgacaaa ggaggcaaca
    70921 agatatggta aatctaaaat gcttcggtat gttgctggcg agggcgaacc tattttccct
    70981 gttggatata tccaacacag aaaaccaatg gcaaaggtgt atttagcgaa ttgctacact
    71041 ctagagggaa gaaaatttat ccacaccaat ctaagtgttg ataaattact gatgtaccag
    71101 ttaatgaaat tatctttgga aaatcgcaca atagaatacg cagacaatcg aatttcgcta
    71161 ttttctgctc aacatggaaa atgtgctata actctggaag aatttcaaca agctaatgaa
    71221 atacattgtc atcatattgt accaactgga gctggtggaa atgacgatta taaaaatctt
    71281 attttggtaa aagaggcggt acaccgtcta atacacgcta aaacagaaga aacaatacaa
    71341 aaatatatag ctctattgaa gctaaataaa gtccagctca taagactaaa taaatataga
    71401 gtattagctg gcaatcaaga attaaacttg atataggtct actacataaa tacttttcaa
    71461 tataaacacg atggaacgcg gagtgcggtg aaagtcgcac gctccgtgtg aatgggggga
    71521 aaagtttgag atatttataa tatcaggaac ttacctatcc atatatcaat ggtttttaac
    71581 caatgcccaa tccttagtct tactggcaat tgtggtcatt ggtttgtttt taggatttaa
    71641 gcgggagttt tccaaactga ttggcttttt ggtgattgct ttgattgcgg tcggattggt
    71701 ctttaatgct tctggggtga aggatgtctt actcagtttg tttaaccgaa ttgtcggtgc
    71761 gtaaggggga agcacgatgg aacagatgcg tgtttatatt gcgaacctcg ggaaatataa
    71821 cgaaggtgaa ttggtggggg cgtggtttac cccacctgtc gattttgatg aagtgaaaga
    71881 acgaattggg ttaaatgacg aatacgagga atatgcaatt catgattatg aacttccctt
    71941 tgagattgat gagtatacgc caattgagga aattaatcgg ttgtgtggct tagcggagga
    72001 acttgaagga acgccagttg gtgaagttgc ttctgagatt caacatgctt tcttcaattc
    72061 ttttgaggag atggtagagc atgtggatga tattatctat tatccagatt gcgatgatat
    72121 gagtgatgta gcatatttct tgattgtaga agctggtgat tttggagaag taccagaaca
    72181 cttgaagacg tatattgatt ttgatgcgtg tggtagagac ttggagattg gaggcaatta
    72241 tttggtaacg agtcgaggtg tttttgagta tccatattaa caaaattatt gaagggtagt
    72301 tgatttctaa actatccttc tttttaaagg aatgatttga tgaagaaaat aaaaagctac
    72361 accagtattt gggcagttga aaaggtcatt tatgcaatta atgactttca actgcctttt
    72421 cctgtgactt ttaaccagat ggcttggttt gttttatcct tgctgtttgt catcgtattt
    72481 gcccatgtgc ctccactatc tatgatagag ggggcctttt taaaatactt aggtataccg
    72541 gtggcagtta cttggttcat gtcgcaaaaa acgtttgacg gtaagaaacc attgggattt
    72601 ttacgatctt ttattagcta tcacttacga tttaaggtca ctttcgcagg aaagaaagtc
    72661 aaagaacaga agaaacgatg ggacgaaccc attactctgg taaggagtgt gaactatgta
    72721 cccgattaag tatattgaaa ataatcttgt ctttaatcaa gagggagaat gttttgccta
    72781 ctatgagtta ataccttaca attactcctt tttgtcacct gaacagaaat atcaagtcca
    72841 cgataacttt cgtcaattga ttgcgcaaaa tcgtgaaggc aagattcatg ccttacaaat
    72901 tgccacagaa agtagtattc gtgccacgca agaacgatca aaaaaggaaa ttaccgggcg
    72961 attaacagaa gtagcgaaac aacgaattga tttacagaca gaggcattag tatccatgat
    73021 tggcgatagt cagattgatt atcgcttttt tattggcttc aaactgattg cgacagatga
    73081 agaggtcaat ctaaagaatc tcaagaaatc tttcttctcc ggctttcaag aatttgtcta
    73141 tggagtgaat catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgccg
    73201 atataccaag cttgaaaaac taatggaaag taaattggct cgaagattta aagtcagacg
    73261 agtcacaccg agtgatctga tgtatttgat tgaacatatc tatggcgaaa agggtacacc
    73321 atttgaagaa tatgaatttc agctaccaaa gaaaaagctg aagtcggaaa ctttggtgaa
    73381 acgttacgac ttattacgtc ccagtcgttg tttgattgaa gaaaagcccc ggtacttatg
    73441 catggaacat gagaatcatg aatcatatgt agcgtactta acaattaata caattgtggg
    73501 ggaaatggag tttccctcct cagaactttt ctattaccag caacaacaat ttacattccc
    73561 aattgacact agtatgaatg tggagattgt gacgaacaaa aaggcacttg ctaccgttcg
    73621 caataagaaa aaggaattaa aagacttgga caaccatgcc tatcaatctg acaatgaaac
    73681 aaattccaat gtcttagatg cattggattc cgtagatgaa ttggaaacta ccttggatca
    73741 gtccaaagaa tccatgtaca aactgagcta tgtggttcga gtaagtgccg aatcagtaga
    73801 tgaactaaag cgccggtgtg atgaagtgct agatttttat gatgatacca atgtaaaact
    73861 agtccggcct tttggcgata tgatggggct acatgaagaa ttcttacctt ctagtaagcg
    73921 atacatgaat gattatatcc agtatgttac gtctgatttt ctcgccggac ttggttttgg
    73981 cgccactcag atgttagggg agttggaagg catttacttt ggttataacg tagatacggg
    74041 gcgcaatgtt tacctgaaac cggcattggc ctcacaaggg gtcaaggatt cagttaccaa
    74101 tgcgctagca gcggcattcc tcggttcact cggtggcgga aagtccttta gtaacaatct
    74161 cttagtttat tacgccgtcc tttttggtgg
//
LOCUS       DACERL010000004        69251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.004, whole genome shotgun sequence.
ACCESSION   DACERL010000004 DACERL010000000
VERSION     DACERL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69251
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.004"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            70..420
                     /locus_tag="IXF54_000251"
     CDS             70..420
                     /locus_tag="IXF54_000251"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1403536.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            436..1557
                     /locus_tag="IXF54_000252"
     CDS             436..1557
                     /locus_tag="IXF54_000252"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1403537.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            1568..1930
                     /locus_tag="IXF54_000253"
     CDS             1568..1930
                     /locus_tag="IXF54_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1403538.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2106..2375)
                     /gene="rpsN"
                     /locus_tag="IXF54_000254"
     CDS             complement(2106..2375)
                     /gene="rpsN"
                     /locus_tag="IXF54_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1403539.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            2565..3512
                     /locus_tag="IXF54_000255"
     CDS             2565..3512
                     /locus_tag="IXF54_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1403540.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYHLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            3818..6523
                     /locus_tag="IXF54_000256"
     CDS             3818..6523
                     /locus_tag="IXF54_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1403541.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            7120..8943
                     /locus_tag="IXF54_000257"
     CDS             7120..8943
                     /locus_tag="IXF54_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1403542.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9085..9270)
                     /locus_tag="IXF54_000258"
     CDS             complement(9085..9270)
                     /locus_tag="IXF54_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1403543.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            9745..10008
                     /locus_tag="IXF54_000259"
     CDS             9745..10008
                     /locus_tag="IXF54_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1403544.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            10008..10274
                     /locus_tag="IXF54_000260"
     CDS             10008..10274
                     /locus_tag="IXF54_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1403545.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            10475..10963
                     /locus_tag="IXF54_000261"
     CDS             10475..10963
                     /locus_tag="IXF54_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1403546.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPKDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            11026..11193
                     /locus_tag="IXF54_000262"
     CDS             11026..11193
                     /locus_tag="IXF54_000262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403547.1"
                     /translation="MFLAKDEDGKFPIVEKMIEVTDTAVTSFDDAAEGVEQSRTDGKG
                     LNESHAKGCKN"
     gene            complement(11505..12176)
                     /locus_tag="IXF54_000263"
     CDS             complement(11505..12176)
                     /locus_tag="IXF54_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403548.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(12173..13090)
                     /locus_tag="IXF54_000264"
     CDS             complement(12173..13090)
                     /locus_tag="IXF54_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1403549.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(13087..13728)
                     /locus_tag="IXF54_000265"
     CDS             complement(13087..13728)
                     /locus_tag="IXF54_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403550.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(13732..14907)
                     /locus_tag="IXF54_000266"
     CDS             complement(13732..14907)
                     /locus_tag="IXF54_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1403551.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            15100..16131
                     /gene="queA"
                     /locus_tag="IXF54_000267"
     CDS             15100..16131
                     /gene="queA"
                     /locus_tag="IXF54_000267"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1403552.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            16750..19389
                     /locus_tag="IXF54_000268"
     CDS             16750..19389
                     /locus_tag="IXF54_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1403553.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(19557..20255)
                     /locus_tag="IXF54_000269"
     CDS             complement(19557..20255)
                     /locus_tag="IXF54_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1403554.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            20473..21618
                     /gene="tgt"
                     /locus_tag="IXF54_000270"
     CDS             20473..21618
                     /gene="tgt"
                     /locus_tag="IXF54_000270"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1403555.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21715..22095
                     /gene="yajC"
                     /locus_tag="IXF54_000271"
     CDS             21715..22095
                     /gene="yajC"
                     /locus_tag="IXF54_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1403556.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            22400..24997
                     /gene="adhE"
                     /locus_tag="IXF54_000272"
                     /gene_synonym="adhC"
     CDS             22400..24997
                     /gene="adhE"
                     /locus_tag="IXF54_000272"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1403557.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIICDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            25226..26332
                     /locus_tag="IXF54_000273"
     CDS             25226..26332
                     /locus_tag="IXF54_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1403558.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            26349..27377
                     /locus_tag="IXF54_000274"
     CDS             26349..27377
                     /locus_tag="IXF54_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1403559.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDTFKNDPAYKDLY
                     QKIQEQGA"
     gene            27383..27862
                     /locus_tag="IXF54_000275"
     CDS             27383..27862
                     /locus_tag="IXF54_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1403560.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLSGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSIPVSGILIIIYSLINLIDTLSNKPAIQEKI"
     gene            27878..29167
                     /gene="kduI"
                     /locus_tag="IXF54_000276"
     CDS             27878..29167
                     /gene="kduI"
                     /locus_tag="IXF54_000276"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1403561.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKF
                     YISSVPAHHKYPNVKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTIL
                     EPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPS
                     WSIHSGVGTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            29187..29990
                     /locus_tag="IXF54_000277"
     CDS             29187..29990
                     /locus_tag="IXF54_000277"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1403562.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGATIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(30239..31303)
                     /locus_tag="IXF54_000278"
     CDS             complement(30239..31303)
                     /locus_tag="IXF54_000278"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1403563.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLASLGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(31300..32385)
                     /locus_tag="IXF54_000279"
     CDS             complement(31300..32385)
                     /locus_tag="IXF54_000279"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1403564.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(32398..33375)
                     /gene="mvaD"
                     /locus_tag="IXF54_000280"
     CDS             complement(32398..33375)
                     /gene="mvaD"
                     /locus_tag="IXF54_000280"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1403565.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(33368..34312)
                     /gene="mvk"
                     /locus_tag="IXF54_000281"
     CDS             complement(33368..34312)
                     /gene="mvk"
                     /locus_tag="IXF54_000281"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1403566.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            34634..35287
                     /locus_tag="IXF54_000282"
     CDS             34634..35287
                     /locus_tag="IXF54_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1403567.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            35372..36277
                     /locus_tag="IXF54_000283"
     CDS             35372..36277
                     /locus_tag="IXF54_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1403568.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASHILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(36279..36911)
                     /locus_tag="IXF54_000284"
     CDS             complement(36279..36911)
                     /locus_tag="IXF54_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1403569.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            37231..37755
                     /gene="infC"
                     /locus_tag="IXF54_000285"
     CDS             37231..37755
                     /gene="infC"
                     /locus_tag="IXF54_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1403570.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            37827..38027
                     /gene="rpmI"
                     /locus_tag="IXF54_000286"
     CDS             37827..38027
                     /gene="rpmI"
                     /locus_tag="IXF54_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1403571.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            38080..38439
                     /gene="rplT"
                     /locus_tag="IXF54_000287"
     CDS             38080..38439
                     /gene="rplT"
                     /locus_tag="IXF54_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1403572.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKDALAK"
     gene            38691..40028
                     /locus_tag="IXF54_000288"
     CDS             38691..40028
                     /locus_tag="IXF54_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1403573.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGLAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            40076..42205
                     /locus_tag="IXF54_000289"
     CDS             40076..42205
                     /locus_tag="IXF54_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1403574.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     PGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            42180..42869
                     /locus_tag="IXF54_000290"
     CDS             42180..42869
                     /locus_tag="IXF54_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403575.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(43276..43962)
                     /locus_tag="IXF54_000291"
     CDS             complement(43276..43962)
                     /locus_tag="IXF54_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1403576.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(44098..44292)
                     /locus_tag="IXF54_000292"
     CDS             complement(44098..44292)
                     /locus_tag="IXF54_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403577.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(44342..44782)
                     /locus_tag="IXF54_000293"
     CDS             complement(44342..44782)
                     /locus_tag="IXF54_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1403578.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(44786..45631)
                     /locus_tag="IXF54_000294"
     CDS             complement(44786..45631)
                     /locus_tag="IXF54_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1403579.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            45780..47198
                     /gene="pepV"
                     /locus_tag="IXF54_000295"
     CDS             45780..47198
                     /gene="pepV"
                     /locus_tag="IXF54_000295"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1403580.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(47255..48202)
                     /locus_tag="IXF54_000296"
     CDS             complement(47255..48202)
                     /locus_tag="IXF54_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1403581.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(48425..48775)
                     /locus_tag="IXF54_000297"
     CDS             complement(48425..48775)
                     /locus_tag="IXF54_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1403582.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            48975..50054
                     /gene="pepA"
                     /locus_tag="IXF54_000298"
     CDS             48975..50054
                     /gene="pepA"
                     /locus_tag="IXF54_000298"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1403583.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            50197..50517
                     /locus_tag="IXF54_000299"
     CDS             50197..50517
                     /locus_tag="IXF54_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1403584.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            50539..51003
                     /locus_tag="IXF54_000300"
     CDS             50539..51003
                     /locus_tag="IXF54_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1403585.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            51208..51813
                     /locus_tag="IXF54_000301"
     CDS             51208..51813
                     /locus_tag="IXF54_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1403586.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLGADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWSGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(51852..53138)
                     /locus_tag="IXF54_000302"
     CDS             complement(51852..53138)
                     /locus_tag="IXF54_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1403587.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            53566..54045
                     /gene="rlmH"
                     /locus_tag="IXF54_000303"
     CDS             53566..54045
                     /gene="rlmH"
                     /locus_tag="IXF54_000303"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1403588.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            54440..54916
                     /locus_tag="IXF54_000304"
     CDS             54440..54916
                     /locus_tag="IXF54_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1403589.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            54931..55731
                     /locus_tag="IXF54_000305"
     CDS             54931..55731
                     /locus_tag="IXF54_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1403590.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            55715..56533
                     /locus_tag="IXF54_000306"
     CDS             55715..56533
                     /locus_tag="IXF54_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1403591.1"
                     /translation="MILMNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYG
                     LEKALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSF
                     KTGMMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLF
                     SIGYTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPI
                     QTEILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            56546..56965
                     /locus_tag="IXF54_000307"
     CDS             56546..56965
                     /locus_tag="IXF54_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAQ1403592.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSSIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            56969..58639
                     /locus_tag="IXF54_000308"
     CDS             56969..58639
                     /locus_tag="IXF54_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1403593.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            58746..59501
                     /locus_tag="IXF54_000309"
     CDS             58746..59501
                     /locus_tag="IXF54_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1403594.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            59907..60839
                     /locus_tag="IXF54_000310"
     CDS             59907..60839
                     /locus_tag="IXF54_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403595.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            60964..61221
                     /locus_tag="IXF54_000311"
     CDS             60964..61221
                     /locus_tag="IXF54_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAQ1403596.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            61453..61857
                     /locus_tag="IXF54_000312"
     CDS             61453..61857
                     /locus_tag="IXF54_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAQ1403597.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            61876..64533
                     /locus_tag="IXF54_000313"
     CDS             61876..64533
                     /locus_tag="IXF54_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1403598.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            64605..65081
                     /locus_tag="IXF54_000314"
     CDS             64605..65081
                     /locus_tag="IXF54_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1403599.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            65095..65883
                     /locus_tag="IXF54_000315"
     CDS             65095..65883
                     /locus_tag="IXF54_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1403600.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            65885..66682
                     /locus_tag="IXF54_000316"
     CDS             65885..66682
                     /locus_tag="IXF54_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1403601.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            66701..67096
                     /locus_tag="IXF54_000317"
     CDS             66701..67096
                     /locus_tag="IXF54_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1403602.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            67098..68219
                     /locus_tag="IXF54_000318"
     CDS             67098..68219
                     /locus_tag="IXF54_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1403603.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            68239..69108
                     /locus_tag="IXF54_000319"
     CDS             68239..69108
                     /locus_tag="IXF54_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1403604.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
BASE COUNT        22170 a        12249 c        14527 g        20305 t
ORIGIN      
        1 atttgtggca aaatggttat aatttggtag aatgagagtg ttctaaattt attgaaaaga
       61 gaaaagcata tgataaaagg aattgggatc gatgcagtcg aacttcctag gatcactcgg
      121 ctgatcgaag aaaaaccaaa attcattgca cgaatcttga cctctgatga aatgaaacta
      181 ttccagtcct tgccttttca tcgccaagta gaatttttag gcgggcgtta cgcttgcaaa
      241 gaggctttct ctaaagcttg gggaactggg atcggaaaag ttacctttca agatgtcgag
      301 attttaaaaa atgaaaacgg tcagcctgtt gtgacgcgtt cacctcatga aggcaatgta
      361 tgggtcagta ttactcatac aaatgaaaca gcttttgcac aaattatttt ggaagactag
      421 aaagaaggaa aaacgatggt tgtcgcttgg caccgcccaa cgaaagcagt gatacataaa
      481 aaagctataa ctgaaaatgt agcaaatgaa gttgcaagac ttccgcaagg aaaagaattg
      541 tttgcagtag taaaagcaaa cggatacggt catggagcta ttgaaacagc tgaggcagca
      601 gttgcaggtg gagcttcggg cttttgtgtt tcaaatttag atgaaggagt cgagttgaga
      661 gaagctggat ttactcagcc catccttatc ttgaatatgg ttccttacga cgcactgact
      721 gtagctgttg ctcatgattt atcagtaact gcaggtacac gggaatggtt gcaggcagca
      781 gctgctgttt tagagaaaag taagctagag acgcctcttt cgattcatct aaaagcagac
      841 actgggatgg gtcgtatcgg attttgtaca cctgaagaag taaaagaggc agctgcattc
      901 atcaaagaaa gtcgtgtatt agaatgggaa ggtttattta cccatttttc tacagcagat
      961 caggcagatg atacatattg gaatctgcaa aaagaacgct ttatagaagt gctaaaaaaa
     1021 cttcctgaac ttcctcgata tgtacatgtc agcaatagtg caacggcttt atggcatgat
     1081 gaaacaatcg gcaatatgat tcgttatgga gtagcaatgt acggactgaa tccctctggg
     1141 cacgcactac cggaagttta tccattgcag ccagcacttg agctggtttc tgaattgatt
     1201 caagtcaaaa aactcccagc aggtgaagga atcggctatg gagaaacata tatcactcct
     1261 gaagcagagt ggatcggaac gataccgatc ggttatgcag atggctggcc acgaaagatg
     1321 cagggttttt ctctattggt agaagggaac tactgtgaaa cgattgggcg tgtatgtatg
     1381 gatcaactga tgatccgatt gcctcaagaa ttccctgtcg gaacaaaagt gacgcttatc
     1441 ggcaaaaatg cagacaaaga aatcactatg caggatatag cagatcaatt aggtacgatc
     1501 cactatgaag tggcttgcgg attaggacag cggataccaa gagaatatca agaataagga
     1561 ggatcctatg gtaaaaagag gagatattta ttttgctgat ctttctccag tcgtgggatc
     1621 ggagcaaggc ggaacacgcc cagttttggt tattcaaaat aatctaggta accattttag
     1681 cccgacgatc attgttgcgg ccataacggc aaaaatggct aaacctaagc ttccgactca
     1741 cattgggatc aaagctactg gaacaggaat agaaagagac tccgttatcc tgttggagca
     1801 gatcagaacg atcgataaat caagattgaa agaaaaggtc tgccatttgg atcgacccat
     1861 tatggcagaa gttgaccgag cattgaaaat tagtgtgggt atcgaagcac tgactcctgt
     1921 tactaaataa atatagcgaa gtaaccatgc agcctttaca tataaaaaag gaccagctgt
     1981 gcgtaaaaaa agatacgtct gcttctagta ccagcatttc tgctagaaga agcgaacgta
     2041 tcttttttga tgatccaagg agtggtccat gcgccctcct cctttacatt acagccagac
     2101 ttggattacc agctggcttt ttttactcct ggaatcaaac ctttatgtgc taattcacgg
     2161 aattttatac gggacatccc gaatttacgc atgtatccgc gagggcgtcc atcgattaaa
     2221 tcacgatttt tcaaccgttc tggacgagca tctaaaggaa gtttgcgcaa gccttcgtaa
     2281 tctttttgcg cttttaattc ctggcgtaaa gcagcatagc gttcaatcgt ttcttgctgt
     2341 ttttttgctt tggcaatctt tgatttttta gccatcaaca aattcctcca attatttttt
     2401 attagcgtaa atcgtaatct ttacgctttt agataaataa tttaacacgt ttcgcgaaaa
     2461 aagacaacta ttttgttttg aatcatgcgc caaaacgaaa agacattttt tttgtcaaaa
     2521 taaaggcata aacgtaaact tatagaaaaa gggagatggt gaccatgcga agatttgcaa
     2581 ttaacggtta cggagaagca caagatgttt ttgaagaaat cgatgctcat cccagagaag
     2641 tcggttctgg acatgttcgt gtagaaataa aagcattcag tgtgaatcct tatgatgttg
     2701 ccttgcgttt aggagaaatg aaagagatac gaacattgaa attcccttat gtaccaggta
     2761 atgatggcgc tggtattgtg acagagattg gttcagatgt cacaactgtt catgtaggtg
     2821 atcgagtagc tgtccatgca gtcggcggga cttacggaga agaagtcgtg ttgccatcag
     2881 caaaagtagc aaaaattcca gataaaatga gttgggaaga agcagccgga atggtcacgc
     2941 caggaattac ggcttatcat ctaatcaacc atttgacgga aatccagccg acagatactg
     3001 taatgatact aggagcatca ggtgcggtcg ggtcaagtct gatccagctt ctccatgaaa
     3061 agggaatccg aatcttaacc agtgcatcca gtaaaaatga ggaaaaggta aaaaaattag
     3121 gagcttcagc atttgctgct tatgataaaa caaatccagg tctgcaattt gctgatcagg
     3181 ctgaccttgt aatcgatgcg acgaaaggaa gtatcaaagg agaaaccggc atccagatca
     3241 tgaaaccagg cggcaggtat gttgcattaa atgacctccc agatctagat ttgcggcaaa
     3301 aaaaggaagg cttttatgaa tcgtttgttc caagaaagga atatctagat gcagaagcct
     3361 ttgctggcat aatcaaagcc taccaaaaag gagcctttca tgtttttatt tcaatgaatc
     3421 tgtctgcttc acttaagcat gtcatccaag cacatcagct agttgaagga catcctcctg
     3481 ctgggaaaat catcctgtca ttcgaaaaat aagaaataca ggaataaaaa acgaaaaaag
     3541 ctgaaacatt tttgtagggg caaagacaaa aaatgtttta gcttttttgc gtatacgtat
     3601 aatagataaa aactttggca taaaattatc tttccctttt atttcatgat aaagtagagg
     3661 ctgtaatcct taattatttc tcaatatgta agagattaca atgagacaat tgccgtgata
     3721 tgtccaaaaa ttcgactttt ttgaaaaaag ccgattgaaa aaagtatgaa atacatggta
     3781 aagtaattca gctgaaaaaa gaagaagggg gagaactatg cagtcaatca aaaatacgct
     3841 tcgattatat ttgcttgact ggaaacggat tttcaaaaac ccgattgcaa cacttctgat
     3901 catcgcgatc atgatcatcc cttcgctata tgcgtggttt aacatcaaag cattatggga
     3961 tccatatgga aatactggag agctgccaat tgctgtttac agtgcagaca agccagcaga
     4021 gtttcaaggt aaagaagtgg ccattgggga gcaagtcatc gaatcgcttc ataaaaataa
     4081 acaattagga tggcaattcg ttgattcaaa agaacaatta gaagacggag tccgttccgg
     4141 aaagtattac gcaggtattt acttgccaaa ggatttctca gaagatctgc tgagttttac
     4201 tagtggtgat atcaagaaac caaaaatcga atatacggta aatgaaaaaa tcaatgcgat
     4261 tgcaccgaaa atcaccgata aaggagcaag ttcgatccaa tcgcaaatca caaatgaatt
     4321 tatcaaaaca gccagttcga ctttattgaa agtattcaat gagataggtt acgacatcga
     4381 tacgaaccta gtaagtatca ataaagtcaa agatatgatt ttatcaacag atgaaaattt
     4441 ggacacgatc gacggctaca caaaacaagt cctcgaatta caaagtcaac tacctgaaat
     4501 caaggaaaaa ctgaataaag caaatgaatt cgtggattac atccctaaag tagatgagat
     4561 gggtgaaaaa gtcgttgcat tgaatgacaa aatgcctgag ttgaaagatc aagcaaaaat
     4621 catccttgat ttgcaagaaa aaatcccaga gatccaaaat gccggtaaac aactagcaga
     4681 gatcgacggc gattttgctt cgatccaaga gacaatgaac caaggaatcg atgaagcaaa
     4741 acaaggcttg acgatcatcc aacaagtaca ggatatcttg ccagatgtga aaaatttaga
     4801 aaatcaggca agtgatctag caaaccaaac aaaatcaggt gcctctcagc tgaaagatgc
     4861 gataccaagc atcacaaaca gtgtagatgt tacattgaag tcaattggac aagtggcaac
     4921 aacgacaggt tcgttaatag atactgtaag aacagctatt gaagatggta agctgactga
     4981 tgatgaaaag aaaaacatca gcaatgtatt gaacggcttt attgacggaa tatctcaaca
     5041 acaaaatgca atcgataaac tggtcgagtt cctgactaaa ttgcaaacaa gtgctggaaa
     5101 tacgaaattg cagccaatta ttgatcagct aaatcaatta aaacccttgc taagcaatct
     5161 gcaaaaccgc ctatctgctt tgaacacagc tgttcaaaat gaagacacga aggctattga
     5221 tgaattgcta gatcaaattg atgccgttgt taataatatc aatggcatca ttgctggtat
     5281 caatacaggt caaatcagtt ccactgtcac ttctctatta gaccaagtca tcgatacgat
     5341 tgggactgca caaggggcgc taagcaaagc ggaccagatc gactttgatt cgcttctcag
     5401 ttcaacgaaa gcaactgtaa cgaatgcagt ggcaatttta gaaaaatatc aaaaagaaat
     5461 gcctgcaatt ggacaagaaa tccatgatgc caatacaatg ctgaatggaa atatggatac
     5521 catcgtcaat ggcatcaatg aaggggcaga cctatacaac aatgaattac cgatccttca
     5581 gcaaaaatta ggattggccg ctgattttat ccaaaatgat tggccagcac taaaaaatga
     5641 aattaccggt acattgacaa cagtaaatga aaaaatgcca gaagtcgaaa cagctgtgaa
     5701 cgctgctgcg gacttgatca acaatgactg gccaaatatc aaagctggta tccaaaaagc
     5761 agcagacgcg atccgaaaag gcgaacaaga agtcgatcta ggcgaagtca tcaaattatt
     5821 gaaactagat gcaacaaaag aaagtgactt ctttacacaa cctgttgagc tacaaaccaa
     5881 tgcgctttat ccaatcgcaa ataacggttc agctagcacc ccgttttata ctgcactatg
     5941 tctgtgggtc ggcgctgttc ttctttcaag tgttgctacg acagaatact atttaggaaa
     6001 gaaagaccga aacaatttta cgaaacgaga acaattcgtt gctcgtatgc tgacattttt
     6061 gacaatggcg attgcgcaaa gcttgatcgt tacattaggg aatattttct tactaggagt
     6121 agatgtccaa aatccggtct acagcgtgtc atttgctgtc ttgatcgcct tagcgttcat
     6181 gatgatcgtc tatgtcttgg ctgcattatt cgataatgtc ggtaaaggga tcgctattat
     6241 catcttagta ctgtcgatct ctggcggagg cgggaactat ccgatccaag tctcagggaa
     6301 attcttccaa atgatcaatc cgttcttgcc atttactcat gcggtaaact tgttacggga
     6361 atctgcagga ggaatttatt ggccaaatgc tacgaaagca atctggatca tgatcggtct
     6421 gtttgttgtc tttggaatcg taggaacggc aatctatccg ttcatcgaag aaaaaatgac
     6481 gaaattcaaa gaagcggcgc atgagagcca tatttttcat taaaaaaaga ggttgtgaaa
     6541 tcagcgtttt gacctgagta ttagagaaaa aaatggaaaa atagttcctc atattttgcc
     6601 gttttttgag ctaatgccga gggtcagctg attgaacacc gtgtaccaag aggttgtgaa
     6661 aaggctgttc tgcatcaagt aataagaacg accaaataaa aacagcttct tatgttttta
     6721 atttggttgg gcttattacc gcagatgcaa gcttttaaac accgtttatc aagaggttgt
     6781 gacaactgcg ctatgcttcg agtagtaaga atgatgggta gaaagcaacc ttacatctct
     6841 ctttcaaaag ttaagatatc aaaaaggata gaacttgatt ttctaagtca agttctatcc
     6901 ttttattata gaattcaaag aaaaagccca taaaaaaatg ttagattttt gctgtattaa
     6961 agcgtttgaa aaagcgcttg atatttttct gtttttttcc aattattttc ttaaagttta
     7021 ttgactttta aggtcagtga gcgtactata acatcttgtt tactataaat tttcgactat
     7081 aaaacttgaa gtaaaattgt gtgtaaagga gtttagaaat tggattactt aaaaaggttg
     7141 ttagtaggta agcctttaaa atctgctgaa aatgatgagc acaaattaac ccgatttgct
     7201 gctttggcat tattgtcgtc tgatgcgtta tcatctattg catatggtac ggaacaaatc
     7261 gttgtcgttt tagtcgcttt gtctgctgca gccatctggt attcgctacc cattgccgct
     7321 tttgttatta ttttattgat ttcattaacg ctatcatatc gtcaaatcat ccatgcttat
     7381 cctcatggcg ggggagcata tgtcgtcagc agtgagaatc ttggaaagaa tgctggcttg
     7441 atttccggtg gctctttatt gatcgactac atgttgacag tagcagtatc tgtctcagca
     7501 ggtgctgaag cgatcacatc agcggtgcca gcactctacg gtcatcaagt cgctatttct
     7561 gtgacaatcg ttttgctact gatgatgctg aatcttcgtg ggctgaggga atctgcatcg
     7621 ttcctattat ttccggtgta cacatttatc cttgtgatct cgttactgat cgttgttggt
     7681 ttatataata tcgtgacagg tgctgtgcca ttgcaagcta ctgcacttcc aggagcagct
     7741 gtgccgggtg tttctatcgc tttgatttta cgagcatttt cttctggttc ttcctcttta
     7801 actggggtgg aggcgattag taatgctgta ccattcttta agaagccaag agcaaaaaat
     7861 gccgctgcta cattgacgat gatggcattg atcttaggtt tcttcttcgt ggggatcaca
     7921 tttatcaact attggtatgg gatcgttcct gaaaaagaag tcactgtttt gtctcaaatt
     7981 ggtaaagctg tctttggaca tggtattttg tattacgtcc ttcaatttgc tacagcgctg
     8041 attttagcag ttgctgctaa caccggtttt tctgcttttc cagttcttgc atacaatctg
     8101 gcaaaagata aattcatgcc tcacatgtat caagatcgtg gggatcgact aggctattcc
     8161 aatgggatta tcacactagc gctaggttcg atcgtattgc tgtttatttt ccatggctca
     8221 acagaacgct tgattccgtt atactctatc ggcgtgttca ttccatttgc tttatctcaa
     8281 acagggatgg tcgtcaaatg gaaaaaagaa ggaaaaagat ggttaagcaa gtcgattgcc
     8341 aatattacag gtgcgttcat ctcatatgca atcattgcga ttttgttcgt ctatcggtta
     8401 ggggacattt ggcctttctt catcattatg ccaatcgtta tgttcatttt ttataagatc
     8461 catgatcatt ataaaaaagt agcagaacag cttcgtttag aaaatgatgc aaaactacat
     8521 gattatgacg ggaataccgt tcttgtatta gtcgggaatg tgacacgggt aaatatcggt
     8581 gcgttgaatt acgcacgttc gatcggtgat tatgtcgttg cgatgcatgt ttcgctagac
     8641 gaagatgtcg aaaaagaaaa agaaatcgaa gcagaattta aaaagcattt cccagatgtc
     8701 cgtttttcaa tcgtccactc ttcttatcgc tcgattgaaa atccaattat ccgttatgta
     8761 gatatcgtaa gtaaaaatgc agcgaaacaa aactatacaa ctactgtgtt gattcctcaa
     8821 tttgtaccaa acagacgctg gcagaatatt ctccataacc aaacaagttt gcggttaaga
     8881 ttgcgattgt cttggcgtga aaatatcgtt gtcagcacct atagctacca tttgaagaaa
     8941 taaaccaagt tttaaaatca aaaacagacc ctctagctct taattgaagc tagagggtct
     9001 gttttgtctt attcccagct aaaggtgccc tctataagcc attaaagatg ggaacagcgt
     9061 atgaaatgtt attattcttc ctatttagct gtaaaacatt ttgattttga tgtcttgatc
     9121 ataatttccg aatcctttgc caaataatgt acagcctcct atattttcag cttcttcctt
     9181 tacctcgact cttaagtgcc atgtgtcttc ataagtagaa atctcagcct cgtcaagtga
     9241 ccattttttg ttaccataac tcagtgccat ttttaattgg tgattcacaa acccgacggt
     9301 ctaagcaagt atcaaatcga tagtgaacat gaatggcagg agacgaaaga ggcaatcatt
     9361 tttgaggcag tagaagactt gacggccaaa atcagttttg aataaccgtt acaaatcttt
     9421 cgtgggtatc ctcaggttat tcaagatatc aatcaagtca cactgcaacg aggaaccttc
     9481 gtgtattggt gttgttgcat agactcacag gctttggatt cgacctttgc taaagatgat
     9541 acgttgttac caattacttt acgtttggct accgctagtc gtaaaggagt aagaagtgag
     9601 cgtggacaaa gatcctccct aaaaaggggt ctttttctct atgtaaaaaa gaggaaaagt
     9661 ttgctgaatt taagtgcttt attgtatttt ttgacgtact taatcaagta cgctataatt
     9721 atatcagaaa aaggaggaga gacaatggct aatacaacga tgaatcctag cactgctcgt
     9781 aaaaattttt atcagttatt gaaagaggta aatgaaaatc atactgaaat cgagattatt
     9841 agtgatcgta gtggaaacaa tgctgtattg attgggctag aagactggag agcgattcaa
     9901 gaaacccttt ttcttgagca aacaggtact ttagataaag tacgagaccg cgaaaaagac
     9961 gacagtggct ttacgaatat cgatgatatt gattgggaag ctctttgatg agtaactata
    10021 cagtcgcaat caaaaactct gcgaaagtgg atttaagaaa aatcaagcaa tcgaatttaa
    10081 agaaacaatt tgaagaagtg attcagacat tgaaggaaga tccatatatg cccacgcagt
    10141 cttttgaaaa gctgagacct acacatgaag gcagatactc acgcaggctg aatagacagc
    10201 atcgagtggt ttacaaagtt gatgaggaaa ataaagttgt ggaaatttat tctgcctgga
    10261 cacattatga gtgaagtgag aagagccaac ctttttagat agttaacaaa gagtctcccc
    10321 aaaaaggaga ctcttttttt gttctaaaac caaccttctt ggatttattg catgatttct
    10381 ttaatttgtt cctatatctc cacctttgaa gggtcattgt ttgagtttag gaatggaatg
    10441 ttgacgggtt tatgtaaaac aaggaggaag aagtgtgaca aggaaaatca gtaaaattgg
    10501 agcatcgttg ttagtgttgg cagtggcaac gaccatcgat tatcgccaaa gtgaggcgaa
    10561 gacaattgga aatgtcgttt atcgagaaac agccaaaagt atgttgaaaa aagcaatgga
    10621 caatcaaccg gagtcgtctt attggttccc aaaggattcg ttggcatggt cttttggaaa
    10681 agatacagat acgaaataca ataccagtgt cgttccttta gcagagcggg tatccaaagc
    10741 gacattgccg aacatgaatg tcacacaaac ggaagaagtc aaagtcgtgg cgctttcggc
    10801 catgaatagc agtatcaacg gcaatgcatc acgagggatc gatatctttt acgccaatgt
    10861 cttttcttat tggcaatata tcgatcaatt ggtttacggg ggtgctcttc tggtgaagga
    10921 tcatcgtatc gccaagtcta gttgcgacgg atgcagtcca taagaatcgt gtaccggttt
    10981 taggaaccgc aaacctccca tggcgagaag ctggaatggc tagatatgtt tttagccaag
    11041 gatgaggatg gcaagttccc gattgtagaa aagatgatcg aagttaccga tacggcagtc
    11101 accagttttg atgatgcagc tgaaggcgtc gaacaatcga ggactgatgg aaaaggctta
    11161 aatgaaagcc acgccaaagg atgtaagaat tgatgcccaa ttcaaagaac aagcagacga
    11221 gtatttagag atcatgtggt atgaattcca tgacgagtga tgggaaaatg gattggcaaa
    11281 atgccttagc ggataagaaa aaaacttagt cggtggatgc agagatgaac ccgttggcag
    11341 tatgaagttg gagtatacgg aatggacaaa tagttaaaaa aagcaactct ttgcccataa
    11401 ggagggaaga atcagagtgg attattgcct taaaaaatga cagaactaaa aaagcctttg
    11461 cgaccaacaa atggtcgcag aagcttttgt tttttacaat aacatcagcg tgatgtacgg
    11521 ctggctggat ctagccgtct ttccaaaata cctaaaagcc agtccgtgat gatcgccatt
    11581 aatgcagttg gcaatgcacc tgctaaaata atcgatgtgc catctgttgc attggttcct
    11641 cggataatga tatctccaag accaccagca ccgacgaatg caccgatcgc ggttatcccg
    11701 atggcaacaa ctaacgcgtt tcttatgcct gccatgataa cagaaacaga gagtggtaac
    11761 tctaccatga atagtcgttg tctagcagtc atccccatgc ctttgccgac atcaaggatg
    11821 tttttgtcta cttggatcat cccggtatac gtattcttga tgatcggtaa gagggaatac
    11881 aaaaatacag taacaatcac gacattgact cccaggccta atccaatgat taaaatggag
    11941 atcatggcta gagaagggat cgtctggatg atattcgcca aacgaatcac ccagtttgct
    12001 aatttacgtc tcctagaaat catgatccca accggaatcc cgacaactgc tgcaaagagg
    12061 acaccataga tagaaatcag gaagtgacgg ataaattgtg caaagatata ggagccattt
    12121 tcctggaaat aatagaaaaa ttgttgaaag acattcattt tatccaattg cttcacttcc
    12181 cttcaaaata gtggttttct tgtaagaaac gttgtgctac tactgatggt tccactagat
    12241 tattatctgc ttcgtagttc aattcctgca ttttttccgt actgatggta tgacctaact
    12301 tatttaaggc ttcttttaaa tgaggcgtat cagctaatat cttgttgtct acaactggag
    12361 cagcatcata tggtgggaag aattgacgat catcttccaa catgactaga tcataactgg
    12421 cgatccgtcc atccgtagag tagccaagaa caatatccat ttttcctgct tcgactgcgt
    12481 cataaacaag tccaatctgc ataggtaata tcgaattgaa ggagaagcca taagtttctt
    12541 ggaagccgtc ataaccgtct cctttacgat tgatccaaga agtatctaca ccagctacta
    12601 attcatccgc tacatttttc agatcgctga ctttagatag atggtatttt tgtgcggtgt
    12661 cttttcgaac aagaaagaca tacgtatttt caaaaccata ggacgggaac cacgtttgat
    12721 cgtatctttt ctcaaattca tctttcacgg tagcaaacgc ttttttcgga tctttttcag
    12781 gaggaaggtt caatgtagtc gttaaatctg ttcctgtgta gcgagccgct gaaattgaag
    12841 catctccgtt catcatcgct tgatggttga tagttgtggt agccagattg ttgatgattg
    12901 cggtattttt atctgtataa tgttcaacca ttccggcaac taggcttgct aagatctgtg
    12961 cttctgaagt gattccacct gtgatcgaaa tggtgtcttc atctgtattg ctggcaagac
    13021 cagggaagga acaactcgca agtataagaa gagagaaaag aaccatggtc atttttagta
    13081 tacgtttcat tcttcattct cctctctaag tgcgatcggt gtcagccggt gttccaagag
    13141 acctagaagc caatcagcca atagtgctaa aattgttaca gggatcgtac ctgccaaaat
    13201 gagatctggt tggtaattat tcagtccact aaaaatcaaa tcgcctaaac caccagcgcc
    13261 gatataagag gcaagtgtag cccatgcaat cacgtaaacg gcagctaaac gaataccagc
    13321 catgatcgtt ggcatagcaa tgggcaattc aacggaaaaa atggattgga actcagtcat
    13381 ccccatgcct tttgccacat cccgataatt ccaatcgaca tttttcatcc caatgtacgt
    13441 attacgtaaa atcggcaata atgaataaat aaagagtgcg ataatcgcag gtaatttacc
    13501 tacgccaaat agcgggatca tcaatgccaa taaagcaaga gaagggattg tctgcaaggc
    13561 actggttaag ccaatcacaa tcgtggcagc ttttggaaaa cgtgtcagta agacaccaag
    13621 cggtacagca atgatgatac caatgatcaa agcaagtcct gaaatataaa gatgttcaaa
    13681 accttttgag agaatttctg aaccgtgctg atgaaaaaac tgctgcatag gtcagacctc
    13741 cttagattct tcaggagtag ctgactctgc aacatcagta gttgcgggat cttctcccca
    13801 aatcacatcg tagacgatat ccactaatga tgcacgagtc aggataccta cgactttgtt
    13861 ctgttcatca acgaccggca cgtatttcag cccgcgtttt aaaatgcgtt gcaaggtatc
    13921 acgaagatag gcggattctt ttacgtaaaa aacatgttta ttcaaaatgt cgccgacact
    13981 ggatactttt cctcgttttt tattcaacgt ttctacatcg ataaatcctt ttaatacatt
    14041 cgaattatcg gtaactaaca acgtatctac acgtttttcc ctcatcagtt tgattgcttc
    14101 ttgtaatgat ttttccggtg taatcgtgat tgctgtattt agcataactt cgccgactct
    14161 tgtcgcattt ggcttggctt gtagtaggcg atcttctcca atcaactctt ctacgaaatc
    14221 gtttgcagga tgacgtaaaa tattttctgg tgtatcaaat tggatcacac ggccttcact
    14281 catgatggcg atacggcttg ctagtttaat cgcttcatcc atgtcatggg ttacaaatac
    14341 gatcgttttt cccaaacgct cttgtaaatc tttgacaagg tcttgcaagg aatcacgagt
    14401 gattggatca agcgcaccga atggctcgtc catcaagatg atatcttgat tcgctgcaag
    14461 ggcacgaacg actccaatac gctgctgctg accaccagat aattcatgtg ggtagcgatc
    14521 taacatctca cggggcaatt ccacaagatc aatcattttt tcagctgttt tattccgttc
    14581 ttcttgactg actttcaaca atttttggac aagagtgata ttttcacgga tcgtcatgtg
    14641 aggcatcaat ccaatacttt ggataacata gccgatctga cgtcgcaatt ccactggatc
    14701 aatttcttgg atggattttc catcaatcaa aattttccct tgtgttggat cagtcattcg
    14761 gttgatcatc cgcatcgtcg tcgtttttcc gcttccgctc gtaccgataa aacaaatgaa
    14821 ctcacccttt tcaaatgaaa gattgacgtc ctctacagcg acttttccac ctttataaat
    14881 ttttgacaca ttttgaaatt caatcatggt gccacctctt tttctatttt caatgaagaa
    14941 ctatctatta ctagtatgcc taaaacaatg aaaaaataca aatagggtag tttttttatt
    15001 taggaaaaca aaaaatagaa gtgaaaaagt gctgattttg ccaacagaac gaaaaacatg
    15061 atagaatgat gaagcacatg atagaaagtg agtgacgata tgttaacaac agaagatttt
    15121 gattttgact tgccggaaga attaatcgca caaacaccat taaaagaccg cgatcattct
    15181 cgtttacttg ttttggatcg gaaaactgga gagatggaag ataaacattt ccatgaaatc
    15241 attgaagagt tacagcctgg tgatgcgcta gttatgaata atacacgagt tctgcctgct
    15301 cgtttatatg gagaaaaacc agatactggt gcgcatttag aagtccttct tctaacgaat
    15361 acacaaggag atacgtggga gacattgatc aaacctgcga aacgtgccaa agttgggaca
    15421 agaatctctt ttggtgatgg cagattgcaa gcagtagtga aagaagaatt ggaacacggc
    15481 ggacggatca ttgactttga gtatgaagga atttttctag agattcttga atcattggga
    15541 gaaatgccat tgcctccata tatcaaagaa cgtctggaag acccagatcg ctatcaaact
    15601 gtctatgcga aagaaaatgg ttctgctgca gcgccaacag ctggattgca ttttacgaaa
    15661 gaattacttg atgagatcga agcaaagggt gttgaattag tctatctgac actacatgta
    15721 gggttaggta cattccgacc tgtcagcgta gataaaattg aagaacacca tatgcatagt
    15781 gaattctacc gtttgacgga agaagcggct gagcgattga accaagtacg taaaaatggc
    15841 gggaaaatcg tggctgtcgg aacaacttca attcgaacat tagaaacgat tggaacgaaa
    15901 tttaatggag aaatcaaagc agacagcgga tggactgata ttttcattac gcctggctat
    15961 gaattcaaag tggtagaagc tttctcaact aatttccatt tgccaaaatc gactttagtc
    16021 atgttagtta gtgcctttgc aggaagagaa ttgactttat ctgcttacca gcatgcgatt
    16081 gaagagcgct atcgtttctt tagttttgga gatgcgatgt tcgtgaaata aggttatgaa
    16141 aagccgctag ttttgaataa caaaaaagct ttacaaagat tttatgacaa agaaaaagcc
    16201 gagctctcga taagctcggc tttcttgtac ctcggaagga ttcaaataaa cagaaatgat
    16261 tgtaactttc catcatacat gcaaagcacg gtgttgaatt tcttcatgtt aaacaaaaat
    16321 tttgttttcg ttacatcttc aacatttgaa acatctcttg ttcctattca attttcagac
    16381 gtttacgtta ttcaattgtt acgtatcgca ttacttcttg tttcggatac ttcgtgatcc
    16441 aaacatccat acgcattcac tatttatagg tttgtactct acacgcatgt ctgggtgaaa
    16501 cctgtacttc taacaacttt gtttgcttac actgttctat cgagaaattc ttctgtctat
    16561 ttgatggtat cctccttcct tacatctata taatagcatc ctttggtagc gtttgcaaat
    16621 ataagcactt taaattttac gctttcttaa caaataatcg ataagccctt tatttgaagt
    16681 aaataaaaaa aggatgttat agtaaaataa gtaccttttt ttgtacgaag attttaaagg
    16741 aggaatagga tggaagaata caagcagaca atagaagaag taaagaaggt aacagaagca
    16801 gatccagata ctgggttgtc ttcacaagaa gtggatgaac ggcgaaaaaa gcagggattg
    16861 aataaattcg atgaggcgcc gaaagaatcg atgatcaaaa aattctttcg aagcttgtcc
    16921 gattttacaa cgatcatcct tcttgtagca gcggtgattt ccttttatac cgcatttgct
    16981 acagaacatg gggatttatt tgaaggactg ctgatcatcg ctatcgttgt gatcaactcg
    17041 gtattggcga ttgtccaaga aggaaacgca gaaaaagcac tggaatcctt gcaagatatg
    17101 aataaacaaa cggcaactgt catacgagac ggcaaagtga ccactgttga gtcagagcaa
    17161 ctggtagtgg gtgatattct tgttttagag tccggtgatg ctatttctgc cgatgcccgt
    17221 ttgatcgaag catcacaatt acgtgtagaa gaatcggcgt tgactggaga aagtgaagca
    17281 gttgaaaaag atgcagcatt tattgctgaa gaagatgagt cattaggtga ccaacttaat
    17341 atggtattta aaggatgtac ggttgctgca ggtcgtggga aagcgatcgt cactgccatt
    17401 ggcatggcta cagaaatggg taagatcgcg gatcttttga acgaaaatac tatgcaaaaa
    17461 acacctttac aggtccgatt gaatcagcta ggcaaacgaa tcagtatgat cgctttagcg
    17521 gctgcggctt tagtatttgt gattggtgaa ctgcaaggag aaccactcct tgagatgttc
    17581 atgacttcga tttccttggc tgttgcagct gtaccggaaa ccttgactgt tattgttacg
    17641 ttgacacttg catacggtgt gcagaagatg gcgaggaaac atgcgatcat ccgacagttg
    17701 ccagcagttg aaacgctagg aaccgctaat gttatctgct cagacaaaac aggaacgctg
    17761 acacaaaatg aaatgcgcgt ccgccgggta tggagcaaag aagatgaagt aacgaatatc
    17821 gaagattcaa tgacgaacag cgcgatggaa atattgaaaa tggctgcact atgtacagat
    17881 gtgacggtcg agcaagaaga tgacgacctt gttatcaaag gtaatccaac agaagtagcc
    17941 atcgtaaggg ctgttgaaga gaattatcat acaaaggcag aattagaaga aaaatatcca
    18001 cgagtaaacg agcttccttt tgattctgaa cgaaaaatga tgacaacagt tcaccaaatg
    18061 ggcgaaaaat acatctcaat caccaaagga gcattcgatg ttcttgctcc gcgctttagt
    18121 tcaggcgatg tagaacaagc ggcgatcgtt aatgatcgct ttggtaaacg ggcactgaga
    18181 gtgattgctg ttggttatgc tacttatgac gaagaaccgc aagatatttc ttctgaagca
    18241 ctggaaaaag acttacgttt gattggtctg atcggaatga tcgatccacc acgtccagaa
    18301 agtaaaggag caatcaagcg agcgaaaaag gctggcatca aaacagtcat gatcactgga
    18361 gatcatgtgg tgactgccag tgccatagcg aaagaattag gtatcttgaa tgatccgtca
    18421 gaagcacttt caggatcaga actgcatcaa ttatcagacg aagaattaga tgcgcgtgtc
    18481 aaagctctct ctgtctatgc tcgggtaacg ccagaagaca agatccggat cgtcaaatct
    18541 tggcaacgta cgggtgcagt cgtagcgatg actggggatg gtgtgaatga tgcaccagcg
    18601 ttgaaagcaa gtaacgttgg gtgtgccatg gggatcacag gaacagatgt ggcgcaaagt
    18661 gccgcagata tgattttgac ggatgacaac tttgcgacga tcgtagacgc tgtcagtcaa
    18721 ggacgttcgg tttatcagaa tattcgtaaa gctatcaact ttttgctaag ctgtaatatt
    18781 tcggagatct ttattgtttt gattgcgatg ctgttaggat ggggagcacc atttacagct
    18841 gtgcaactac tgtttgtcaa tgtagttgct gatggacttc caggatttgc tttaggaaga
    18901 gagccagcgg aacacggcat catggatcag ccacccattc cgaaaaatga agggatcttt
    18961 gctcgaggtt tattgcaaaa aattgccata aatgcagggg tcttcaccat cgtaaccttg
    19021 tttggttatt atttaggtag ttatgtcgat actatcagcc catgggtaga cgcaagtcag
    19081 catgtcggtc aaactgttgc attcttgatc ttagcttatt catcgattct gcatgtgttc
    19141 aatgtacgca gcagccaatc gatcttcaaa gtcaatttag cgaccaataa agctttggta
    19201 gaaatggcgt tactggcatt agctattaca acggctgttg ctttgctgcc atttacgcaa
    19261 gaactattcg gcctagtaca catcagtttg aatcactggt tcttggtagg gattttatct
    19321 atcgtaccaa tcgcggtgaa tgaactgatc aagttccatc gattaccgga agcagaagaa
    19381 gaggaatgat taactgtttc taagttgatt ttctttttac tggaactata caaaaaggag
    19441 gtgtgacatg aatgttgtca cacctccttt ttgctaatgg gctgaatgtt tcttttactg
    19501 cctctcgata aacggtgttc tcctgccacc ccctcggtca caagtcacac tctcttttaa
    19561 atatcttttg gcaattgttc ataaaatgct gcttttgttg cagaaatgta tgggctgagc
    19621 catagataac caataccagc agttgctaat gctaagatat gccagccgat aaatgaaaga
    19681 tctaaccaga aaagttgtcc tttataacca tccatcaatt ttcgacttgc agtgatcgta
    19741 tccagtactt taggtgtttg tcctgtttct tggacgatat cgtagtaaat aaaatagctt
    19801 tgagaataag aatagctttt aatgattcca ggaataacta atagcaaacc ccataatgtt
    19861 gtaaagatcg agatcagcac agctaagctg atcactccgc cgataaacaa acctttgaat
    19921 ccacgaaatg catctttgaa tggttcgatc tgatcttttt gtccacgtaa aatatcaaga
    19981 taagtccaag aaataccgct catgaacaaa gcgctaatga tgctggaaat aattccgcca
    20041 ccgcctcctg aactaccgcc tcctgaagtt gtcgcttctg agacgctcgc tgatccatca
    20101 tatatggtgg tggcaaacaa gataatagga atcataataa gaagaatagt agcaatacca
    20161 atcaatgtag ggattagatt tagtaaaaca gcttgtcccc aacgtccttt tagactctct
    20221 tttgcttggc gttttaattc tgcagaagat tgcatgattt ttccctcctt ttctttaata
    20281 acaccaatat accatgtatc aaaaaatatt tctgtcataa tgtttttctt ttttttgtta
    20341 ttcataaaaa aaggataaaa aaaagaatat taaataaagt agaaggaagc cgagggaact
    20401 tgcaaaaagc acttgaaacg attatgatgg tgggagttgt caagatattt tatatagaaa
    20461 gtagggaaat gaatgacgca acctgccatt cgctatcgtt taatcaagaa agaaaaacat
    20521 actggcgcgc gtttagggga attgatcacg ccacacggaa ctttccccac accaatgttc
    20581 atgccagtag gaacattagc tacagtaaaa acaatgtccc cagaagagtt gaaagaaatg
    20641 ggtgctggcg ttattttaag taatacatat catttatggc tacgccctgg tgaagactta
    20701 gtagaagaag ctggcggact gcataaattt atgaactggg atcaaccgat tttaacagat
    20761 tcaggcggct ttcaagtttt ttcattaagt gatatgcgga atatcgaaga agaaggcgtg
    20821 catttcaaaa atcacttgaa cggttcgcgt atgtttttat caccagaaaa agcaatcaac
    20881 atccaaaata aattgggctc tgatatcatg atgagttttg acgaatgtcc accattcgat
    20941 gaaagttacg actacgtgaa aaaatcagta gaacgtacgt ctcgatgggc agaacgagga
    21001 ttgaaagctc atgctaaccc agaccgtcaa ggattatttg gaatcatcca aggagcagga
    21061 tttgaagatc ttcgtcgtca aagcgcgaaa gatctagtcg ggatggattt ccctggttac
    21121 tcaatcggcg ggttatctgt cggggaacct aaatcagaaa tgaaccgtgt attagaattc
    21181 actacaccgt tgatccccga aaacaaacca cgttacttga tgggtgtcgg tgctgctgat
    21241 tcattgatcg atggtgttat ccgtggagta gacatgtttg actgtgtctt gcctactcga
    21301 attgctcgaa atggcacatg tatgacttct caaggtcgtc tagtagtgaa aaatgcgaag
    21361 tatgctcatg atttccgtcc aattgatgag aaatgtgatt gttatacatg taaaaactat
    21421 acacgtgcct atatccgcca tttgatcaaa tgtgatgaaa catttgggat tcgtttgact
    21481 tcttaccaca atttatactt cttgttgaat ctaatgaaac aagtacgtca agcaatcatg
    21541 gatgataatt tgcttgaatt tagagaagcc ttttttgaag aatacgggtt caataaagaa
    21601 aacgcaaaaa gcttctaaag gaaaatcttt taaaaattaa gtaaaaaact agtgttagca
    21661 gcatttttct gctaaagtgt tttttgtaga tacagaatta gtgaggtgaa aaaaatggga
    21721 acattaccaa tgcttgtgat gtttatcgcg ctattagcaa tgtggttttt tatgtcacgt
    21781 tcgcaaaaaa aacaacagca agaacgtcaa aacttgttgg atagcatgaa accaggtgat
    21841 tcagtcgtaa ctatcggcgg attacatgga gttgtttcag aaatcgacaa tgacaaacgg
    21901 acagttgttt tagactgcga agggatcttc cttgagtacg atcgtgcagc aatcaaaacg
    21961 gtcaaaccag ggactgtcgt aacaaatgat gcaacaacta ctgttgaaac aacagaagaa
    22021 ccagctgaga cagttgaagt aaaaactgaa acaacagaaa cgctggaaga gacagaagag
    22081 aagaaagaaa aataagtatc aaagcttatt taaagacgag gtagggcaga aatatgtcct
    22141 acctcatttt ttattcaaaa aagaaagaga taagagtata ggatatattg ctcaatattg
    22201 aataatcggg tgtgtcatat aaataataat aaataacttt atatattttt ctatacaaac
    22261 acctgtaaat aaagcaattt tatatttaaa aataactgtg aaaaaaatca caaaaaagtg
    22321 tttacataga aaaacaaata ttgtatgata tacatgtgaa tgatttaaca aataaccatg
    22381 acccattagg aggataatga tggctaaagt aatggaaaaa gaaaaaacga agacaattga
    22441 cgtacaagca atgattgatg atttggcaga aaaagcaaat gcagcattga aagaaatgga
    22501 aagctttgat caagaaaaag tggaccatat cgtgcatgaa atggcaatgg cagctttaga
    22561 tcaacatatg ccattagcga agatggctgt ggaagaaaca ggccgcggtg tttacgaaga
    22621 taaagcaatc aaaaatatgt atgcgtccga atatatttgg cataatatca aacatgataa
    22681 aacagttggt gtgatcaatg aagatgtcca aaaaggattg attgaaattg ctgaaccagt
    22741 cggtgtggtt tgcggagtaa ctccaacaac gaatccgact tccacaacga ttttcaaatc
    22801 aatgatcgcg ttgaaaacaa gaaatccgat cgtttttgct ttccatccaa gtgcccaaaa
    22861 atcttctgca gaagcagcac gagtagtacg tgatgctgct atcgctgccg gagctcctga
    22921 aaattgtatc caatggatcg aacatccttc gatcgaagcg acttctatgc taatgaacca
    22981 tccaggtatc gcgatcgtct tagcgacagg tggagctgga atggtcaagt ctgcttactc
    23041 tacaggaaaa ccagctttag gtgtaggtcc aggtaacgta ccagcttata ttgaaaaaac
    23101 agcaaaaatt aaacgtgcag tgaacgattt gatcgtttcg aaaacatttg ataatggaat
    23161 gatttgtgct tctgaacaag ctgtgatcgt ggataaagaa atctatgcag cagtaaaagc
    23221 agaattccaa gcacaccaag tttatatcgt aaaaccagat gaattgcaaa aattagaaga
    23281 tgcagtgatg aacgaaggaa aatatgcggt caatccatca atcgttggac attctgcgat
    23341 ggaaatcgca aaattggcag gaatcagtgt accaaaagga acgaaaatgc tgattgctga
    23401 attggaaggc gtaggcccag attatccgct atcaagagaa aaactttctc cagttcttgc
    23461 catgatcaaa gctaacaaca cggaccatgc gtttgatttg tgtgaaggta tgttgaatct
    23521 tggaggatta ggacatacag cagttatcca ttcagaaaac gaagagttgc atgtcaaatt
    23581 cggtcttcga atgaaagctt gtcgtatttt agtgaataca ccatctgcag aaggcggcat
    23641 cggtgatatc tataacgaaa tgattccgtc attgactttg ggatgcggtt cttatggtaa
    23701 gaactctgtt tctcgtaacg tctctgctgt aaacttaatc aatgtcaaaa ctgtagcgaa
    23761 acggagaaat aacatgcaat ggttcaaatt accgccaaaa atcttctttg aaaagaactc
    23821 tctattgtac ttagagaaaa tggaaaacgt tgaacgtgtc atgatcattt gtgaccctgg
    23881 tatggtccaa ttcggttact gcgatacagt tcgtgaagta ttggcacgac gtaaaaatga
    23941 tgtgaagatc gaagtcttct cagatgttga accgaaccca tcgacaaata ccgtatacgc
    24001 aggaacgaaa ctgatggcag acttcaagcc agatacagta atcgctcttg gaggcggttc
    24061 tgcaatggac gcagctaagg gaatgtggat gttctatgaa cacccagaca catctttctt
    24121 tggtgcaaaa caaaaattct tggatatccg taaacggaca tataagatcg acaaaccaga
    24181 aaaaacacaa ttcgtatgta tcccgacaac atcagggact ggttctgaag taacgccatt
    24241 tgcggtcatc actgacagtg aaacacatgt gaaatatccg ttagcagatt atgcattgac
    24301 gccagatgtc gcaatcgtcg atcctcaatt cgtcatgtca gttccagcat ctgtcacagc
    24361 agataccggg atggatgtgt taacacatgc aatcgaatcc tatgtttcag tcatggcttc
    24421 agattataca cgtggattga gcctacaagc aatcaagtta gtctttgact atttagaaaa
    24481 atcagtaaaa acacctgata tggaatcacg tgaaaaaatg cacaacgctt ctacaatggc
    24541 tggtatggcg tttgctaacg cattcctagg tatttgtcac tcagtcgcac ataaaatcgg
    24601 cggggaatac gggatccctc atggacggac aaatgcgatt cttttaccgc atattatccg
    24661 ctataatgca aaagatccac aaaaacatgc gatgttccct aaatatgact atttccgtgc
    24721 agatacagat tacgcagata ttgcaaaatt cttaggtctg aaaggcgaaa caacagagga
    24781 attagtcgaa gctttagcaa cggctgttta tgaattagga aaatctgtcg gcatcaacat
    24841 gagtctaaaa gctcaaggag tcacacaaga aacactagat acaaccgtag accgtatggc
    24901 tgaattagct tacgaagacc aatgtacgac agctaatccg aaagaaccat tgatctctga
    24961 attgaaacag atcatcattg atgcttacaa cggataagca aaagaaagcg tagaaaaaag
    25021 ataagaaaat agatgaaaaa gaacttggaa aacgttgttt ctccaagttc tttttttaat
    25081 ttccaagtaa ctgaaagtta cttttgttat ttaacattta ggtcaatttt agacagtttt
    25141 tgttcaaaaa gcgatattta tagtagacgg tattactgct atattaagga tataaaatga
    25201 aagcgtttaa aaaggtggcg taaagatgat tattaaagaa gagatttttg tacctaaaga
    25261 attagtgaaa gaaaaaattg acctgctcgt tgaaaacctg acaacgatca aagacgataa
    25321 tggtgaattt ttgttggact ttgatggact gaaagtagat gacaagagct ggacagtgtg
    25381 gaattggcca caaggtgtcg gtctatatgg tatttataaa aattaccaga tgacaaaaaa
    25441 tcctcgcgct tatcaagtgg tcaatgaatg gtttgaggat cgtatggaag aaggcgctcc
    25501 gcccaaaaac gtcaatacaa tggcaccgct attgactatg gcttacttgt atgaagacac
    25561 gaaagattca cgctatttgc cttatttaga acaatgggca gaatgggtca tgaatgacat
    25621 gcctcggaca aatgaagacg ggttacagca tgccacatat ggaccggaaa ataagaatca
    25681 gttgtgggac gatacattga tgatgacagt tcttcctttg gcaaaaatcg ggaaactgtt
    25741 gaatcgccca gaatatttag aagaagctcg ttaccagttt atgatccata tcaaatactt
    25801 gatggataaa cgaacaggat tgtggtatca cggctggacg tttgaaggaa atcataacta
    25861 tgccgaagca ttttgggctc gtggaaactg ctggttgacg attgctattc cagagatcat
    25921 tgaaatctta gaattatcga aagaagatgc attgcgcaaa ttattgatcg aaacacttga
    25981 agcacaagtg cgtgcattaa aaacttatca gtctgaaagc ggtttatggc atacgttgtt
    26041 ggatgatcct tcttcttacg tagaatcttc tgcaactgca ggatttgctt acggtatttt
    26101 gaaagcggtc cataaacgtt atttaccaca agaatataaa gaagtcgctt atcgtgctat
    26161 ccaaggattg cttgaacaaa tcgatgaaaa aggagaagtt caaaacgttt caatcgggac
    26221 aggaatgggg gacagtttag atttttatcg gaatatcgga atcacagcaa tgccttatgg
    26281 acagtcgtta acggtgcttt gtttgacaga attgctcgtc tcttattgtt aaaagaaagg
    26341 aaacccatat gaaaaaaaca tcactgctgt ttgcagcaag ttcgcttagc ctaggtctgc
    26401 taggtctaag cggctgcgga aataattcta gtgtggcaac tgtcgcagat gcagacgtaa
    26461 atgagatagt taccttgcgc tttgcttatg caagcaacag ccagccagta attgatgcaa
    26521 tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag atccagtatt
    26581 tcccagatgg acaactgggt ggagaacgtg aactgatcga attgacacag tcaggagcag
    26641 tagatttcac aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat tattcgattt
    26701 tttccgttcc ttatctattc aatgatgaag aacatttctt taaggtgatg gataatgaag
    26761 aaataatgga gccaatctac caatcgacca aaaatttagg atttaccgga ttgacctatt
    26821 atgattctgg tcagcgcagc ttttatatgg tagacggtcc gatccatacg ccggaagatt
    26881 tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg gtagagcttt
    26941 taggcggttc gcccgtacct atgggaagtg atgaagtgta cacgtctctt caatccaatt
    27001 tgatcaacgg atcagaaaac aatgaatttg tcttgtatac agctggacat ggtggtgtgg
    27061 ctaaatacta ttcctatgat gagcatacgc gtgtgccaga tatcatcatc atgaatgatg
    27121 ccatcaaaga acagctaact gatgagcagg aaaaagccat tgaagaagca gcgaaagaat
    27181 caacggtttt tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa gctttagcac
    27241 aagaagaaca tggagtccaa ttcaatgatg tagataatac accgtttcga gaggcagtcg
    27301 caccgcttca tgatacattc aaaaacgatc ctgcctacaa agacctttat caaaagatcc
    27361 aagagcaagg agcgtagctg tgatgaaaat gattttaaat aaggtgttag agatcctagg
    27421 agctgttttg cttgttggga tggtcgctgt cgttttatgg caagtatttt ccagaacggt
    27481 tttaggaaat ccgaatacgg ttacagaaga gcttgtccga ttcggacttg tctggtttgc
    27541 catgttgtct tctgcgtatg ttgtgggaca aaaagcacat ttagccgtaa cggttttaag
    27601 tgaaaaatta tctggtaaaa aaagtaagat tttagaagtc gttgttcaaa gtttatttct
    27661 ggtatttgct gcgatgatca tggtgtatgg cggatggaat gctgtcacat tgacaatggg
    27721 acagatctcg ccttctttaa gcattccgat ggggtacgtc tatttgtcaa tacctgtctc
    27781 aggtatactc atcattatct atagtctgat caatctaatc gatacattaa gcaacaagcc
    27841 agcaattcaa gaaaaaatat aaaaatggag gaataaaatg gcaatagaag ccggattagt
    27901 gttatttttt gtatttgtcg tgttgcttgt attagggatg ccgattgcta taagtattgc
    27961 gttctcatca atgatgactt tattgttagt tataccgttt gatgtttctg cttttagttc
    28021 agctcaaaaa atggtgggaa gtatcaacag tttttcgctc atcgctattc cgtttttcgt
    28081 tctttcaggt attatcatga ataatggagg gatagccaag aaactagtgg attttgcgat
    28141 gttgtttgtc ggacgcgtac cgggggcttt ggctcatacg aatgtgttag ggaatgcttt
    28201 atttggttcc atctctagct cagccattgc cgcttctacc gcgattggcg gagtcttgat
    28261 ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg acagcggtca atatcgcgtc
    28321 agctccgaca ggtatggtca ttcctccaag tactgctttt atcatgtatt cacttgttgc
    28381 tggtggtgct tcgatttcgg ctttatttat gggaggttat ctcgtagggg ctttgtgggc
    28441 actaggaatc atgctagtag cattcatcgt tgctaaaaag aataaatacc ctgttgtcga
    28501 aaaaggacag atggctcatg ctggaaaaat aataaaagag gcgattccta gccttctatt
    28561 gattgtcatc aatatcggtg gaccaggatt catcgagatc gatgggcaaa aagaagaaat
    28621 gaaaaaacaa gacggatact atatcggaaa agaaacacga catgttgtat tttcttctca
    28681 agatgctgaa aacccagcta aattctatat tagttctgtt ccagctcatc ataaatatcc
    28741 aaatgtaaaa atcagtatcg accaagtaaa accaatggaa actggtgaag gcctgacact
    28801 gaatgaacgt aaaatctatc aatacatcca tccaaatgta tgcgaaagct gtcaattaca
    28861 aatgggttat acgattttag aaccaggaag tgcttggaat acgatgcctt gtcatacaca
    28921 tgaacgtcgt atggaagctt atgtctactt tgattatgcc aatgaagata cacgtgtgtt
    28981 ccacatgatg ggcaaaccag atgaaacaaa acatttagtt gtagacaatg aacaagccat
    29041 tatttcgcca agctggtcga tccattcagg tgtaggtaca agcaattact cattcatctg
    29101 ggcaatgtgc ggagaaaata tcacctatac agacatggat atggtgcaaa tggatcaatt
    29161 aaaataagca taagggagag aaaatgatgg aatttagtat ggatatgttt cgtttagatg
    29221 gaaaagtagc attagtcacc ggtgctgtct atggtattgg atttgagatt gcacgctctt
    29281 tagctacagc aggagcaaca atcgtattta ataacttgaa tcaagaatca gttgatgaag
    29341 gtatagcgca ttataaagaa gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg
    29401 atgaagaagc ggttcaagcg atggttcaac agatcaaaga agatgtaggt tcagttgata
    29461 ttctagtcaa caatgcaggg atcatcaaac ggacaccgat gatcgaaatg gatgcggcag
    29521 acttccgcca agtaatcgac gtggatttaa atgccccatt catcgtttca aaagcagtta
    29581 ttccggatat gatcgaaaaa ggcggcggta aaatcatcaa tatctgctcc atgatgagtg
    29641 aattaggacg cgaaacggtc agtgcttatg ctgcggcaaa aggcggatta aaaatgctga
    29701 cgaaaaacat cgcttctgaa tacggtcaat acaatatcca atgtaatgga attggaccag
    29761 gttatattgc tacaccgcaa actgctccat tacgcgaaac acaagaaaac ggtgaacgtc
    29821 atccattcga tcaatttatc gttggacgta cgccagcagc acgctggggc gagccaatcg
    29881 atttggctgg accatctgtc ttcttggctt ctagcgcatc tgatttcgtt aacggccatg
    29941 tcttgtatgt ggacggaggc atacttgcct atatcggaaa acaaccttaa aagagcttta
    30001 gtacactgtt tagtggagag gttgtgaaat cagcggattg aacgaatacc gaaggacagc
    30061 tgattaaaca tcatttattc gaaaaaagag gctgggacag aagcgtttaa ctccaagaaa
    30121 taagaaggaa ttcacgaaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa
    30181 ggagttgctt ttgcttccgc cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt
    30241 acttttgtcc cacgctcttt tctgtgcgtc ttccatactt cactaagtcg atccctcttg
    30301 cttcgcacca atccttcact ggtccggaaa agatcagaga ttgttggtgt agggccgcgg
    30361 tattcgttgc gccgaccata gtataaagca gacgaagctc ttcttgccat tgcttcatca
    30421 gtataatagt ttcttctacg ccatgattca tcagatgcgt cagtacggtt cctgaagtac
    30481 ccactgcatt tgcgccgaga caaagggctt taaatatgtc ataggcattg cggatacctc
    30541 cggaggctag gatttccata gaagtgtctg cttcatttgc ttcaagcaat gaagagactg
    30601 tcgattgccc ccagtcagct aaataactca gttcccgctt ggagcgacgg gcgttttcga
    30661 tctgggtgaa gcttgttccg cttcgaccgc tgatatcaac tgtatgaact cctaaagaag
    30721 ctagatcatt gagtgtttct cttgtcatgc caaaaccgac ttcttttacg atgaggggga
    30781 cgtcaatggc tgtttgagtc tcttgaataa gtactttcca attggtaaaa tcacggtctc
    30841 cctcaggcat aaccaactct tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa
    30901 acaatcggat agcttcttgg gctctttcga cggaagtccc tgcaccgatg ttggcaatga
    30961 ttttcccgtg aggatattct tgtcgcataa tcgtataagt atcggctaaa gaagggtctt
    31021 tcaaggcagc gctgactgaa ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg
    31081 caagatcttg gttgattttt tttgtttttt cgcttccgcc agtcatcgca ttgatataaa
    31141 atgggctgct tagtgtaaac ccaactgcct gtgtacttag atcgacatca gaaactgcta
    31201 tttgcggcaa agggttatgg atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat
    31261 gaaaggcttt agctagtgaa acatgttcgt cttttcgatt catcggtttt cctttctttg
    31321 atcgctatag acatgtaacg gcagtggagt gatttctgct ttttcccatg cactcattaa
    31381 aggaagaatg ccagatttct ggtcaacaat tacgattccg caatcgcccc cacctgcacc
    31441 agaagatttt gcggctcctt catactgttc agctaaatta cacaatttgt tcaaagcagg
    31501 cgtttcgatg acgaccccag tgattgcaga taaatcatgc agtaattgtc ggttttttcg
    31561 aatcatctgt tggatcaacg ttacattatt ttctttaaac cctttgatca tttcgttgac
    31621 acattctgta ctgtttttta agaaaagctg ataagccacc attttatctt ctctcgaacg
    31681 gtgaacttga tcgaccaaat cagaagtaga ggcagggcta cccgtccaac caatcaataa
    31741 acgtaaatct tcaggagcaa tcaatggctc aatggataga cctggccaat ccagggcaag
    31801 taactcactg atagaatgct gagtttcttg ttcttggagc caaggatggt cgaaggttga
    31861 gaaagcgatc cagccaccat aacagctagc agcgatgtcg ccgcaggaac cattatcttg
    31921 aactgctaaa ttggctagtg ctgcaatctt gaaaatctcc aactgagata aattcaacgc
    31981 gtaaaaaaca ttcaaggctt tgaccgttgc aacggtcacg gcaccgctag aacccaatcc
    32041 atatttccgg ccatttgaac tgtctaactc acttgttact ttcagatcat aaaaggataa
    32101 aagaatgttt ttttcttgtg catacttttc agtcaagcga atagcagcaa ggatataatg
    32161 aaaaggattt tcccgaatat ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg
    32221 catcccacta tattgtgcag attggatact tccgactttt cgtgcagatt ctacagtgac
    32281 tgtcacgaat tgatcgactg cagcgataac tgctggatgg cctgtttcaa caactgcata
    32341 ttctccggca atatagagtt tgcctggtgc agatacttca atcataaaaa tctcccttta
    32401 ttcaataatc gcaattcctg gaccagcatg ggcagtaatt acttgttctt tgctaaataa
    32461 tttggtaaat gtttcttgta cctcagataa atggctgttt tccactaaaa ctttgacatt
    32521 agggccagca tccatcgtaa agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc
    32581 atccatcgct tttaagctgt caggagacca gtaagtgaat ggaggctggg cagctaaagt
    32641 cgttccatgc atttttagtc cgtttctttc catcgtttct ccgagaagtt ggaaatcttt
    32701 tgttttgatt gcttgtttca attgatatag atccccttca acggaatcta accagccttg
    32761 ataaaaactg gatgtttcga ctgtccgacg catcccattt ctgctggaca cttctttttt
    32821 ctggtcgttg atcaaaacga aaaccattgc taaatcgtct tcgaaagaat cggaagggac
    32881 tggcttagcg taagaactta agtcgtcatg gcctttttcc cattcgacga aaccaccgaa
    32941 aatacttcgg caagctgaac cagacccgcg tcgagcaaat cttgaaagag agagatcgtc
    33001 taatccaagc ttaagagcag tattgcaagc tcctgctaaa gcagctagcc cgctggcaga
    33061 ggaggctaat ccagctgcgg taggaacgaa attctgactg attactgaag cttttagtga
    33121 gaggccggct tctttacgaa aaaggtcaag aaactgactg actttttttg ttgctttttc
    33181 gtcttgcaag gtgccatcta gataaaattc atctaccata tagctatcag aaaagatgac
    33241 ttctgtctct gtataaaatg catccaatgt taacgaaagg ctgttgttca ttggaaggat
    33301 gagttcttca tttttcttac cccaatattt gattagagca atgttggtat atgcgcgtgc
    33361 tttgccttta aacataggta tgtactccta atccttgtat ccaagtttca gcagcacccg
    33421 catcttcaag tgcttgactg atttcttggg cttttgtttt tgtttgtgct aatgcaatca
    33481 tacagccacc gcgtccaccg ccagttaatt tggcacccaa tgccccggta tcttttgctg
    33541 tttggatcaa tagattaaga aaatcattgc tgattgtcag cttttccaga agtgattgtg
    33601 attcatccat tgtctgtgcc aacgtttctg gtgaattttc aatgattgct tgttttgctt
    33661 gcttcgtcaa gtatcctaat ttttggatca tttgtccagt ttcatgaggc tgggtttcaa
    33721 agaggtgagc gacatctttg acggcttctc ttgtttgtcc tttgattcct gtatcagcga
    33781 caatcaaaaa agcatcgatg ttcaaagaaa agtagtcgaa aggatgccct tttgtaaagt
    33841 aaatcggctg caagctgcta gtagcggctg catcgattcc actaggatta ccgtgggcaa
    33901 ttttttccga aagctggaca ttttctaata gtatttcacg agacaaagga aatgctaagt
    33961 aatcataaaa agcacgagtg actgctgtgg cgactgcagc gcttgatccc attccacgtt
    34021 cagcaggaat cgtgctttca atggtcagtt gcaagttttt ttggatgtca tgctgttttt
    34081 ttaagtgaat gaataatttt ttgatatttt ttaatgcatg aggagcttcg gctaaatttc
    34141 ctgaatagta ggaagaaacc agttgatcgt ccattgcatc cagctcttca aggaatgcgg
    34201 tgacttttgt tgcatagaaa ggaaaggcaa tcgctggttc tccataaaca accgcgtgct
    34261 cgcccatcaa tatgatcttt ccgcttgatt ctccttggcc atagtttgcc ataatctaac
    34321 tccttctcac gtaaaagtga tttttaagca aaaaataaag cgcccaaaac gagttgcgct
    34381 gcttcacgaa tttttttgtc cactaagggt ctcaaaaaac attcctcctt tattttacta
    34441 aaaccatctc agaagtaaaa ctaagaatac gttttctaaa gaaaaaacac ttttttctta
    34501 agaatttttt gacttttttt cttctttgac tagaaaaaaa tacaattgac actactagat
    34561 attgggggta tatttaaaaa ggttggagtt aaacacaata tttagtgatg aagtgagctg
    34621 gaggtaaatg agcgtgatcg ttttagcagg tacaattggt gccggaaaat caagtttaac
    34681 agaaatgatg gctgaacact ttgatagtca ggcattttat gaatcgattg atgataatga
    34741 agtcttgccc ttattttatg caaaccctga acagtatgct tttttgctgc aaatttattt
    34801 tttgaataaa cgttttgcaa gcatcaaaca agcaatgaaa gatgacaata atgtattgga
    34861 ccgttcgatt tatgaagatt cactgctttt ccatctaaat gcagatctag gaagagcaac
    34921 agaaacagaa gttcgtgtgt atgatgaact gctggaaaac atgatggaag aacttcccta
    34981 tgctgcccat aaaaaacatc cggatttatt agttcatatt cgtgtatctt ttgatacgat
    35041 gttggagcgt atcgaaaaac gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct
    35101 atacgattat tacaaagaat tgaacagacg ttacgatcaa tggtatgaag aatacaaaga
    35161 aagtccaaaa atccaaatcg atggagaccg ctataatttt gtggaagatc ctcaagcaaa
    35221 agaagaagtc ttgaaaatga tcgaagaaaa attagcagag attcgccaaa ccaaagtagc
    35281 catttgattt acagttgctc aaataaaggg taaaccttta tttgagcgac ttttttcttt
    35341 atactggaaa caaatgtcag gaggatagag tatgcgggaa aaacactatt ttaatcaaga
    35401 ccgtaatttt tggatcaatg tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga
    35461 agaatatggc attagtgacg agatgctgac ttattctcta gataaaaatg aacgggcgcg
    35521 cgtagagtac gattcttttg atgaagcgtt actgcttgta tcaaatgttc cgcatcagca
    35581 aaaagtcgat aatcattatg aaactagtcc tatcgctttt attttaaaag aagacggttt
    35641 gtttacattt accacgccga ataccgagta cgtgattcga ctaattcgat cgcttttgga
    35701 acgaatgcca gacatgagtg tatacagttt attgtttcgt acattgtttt tgatttctga
    35761 ttcctttttt cctttaatcg aggaagtgaa tagtgaacgt cagcggctta atctgaaatt
    35821 aagagaaaaa acaacgaaca agaatctgct gcaactttca gatttagaga tcggtttggt
    35881 ttacttagtt acaggaacaa aacaaaatgt agtactttta gaacaaatca aggcattggc
    35941 tatttatcgc aagttgtcag aaaaggaaaa agaagagttg gacgatgcac tgatcgaagc
    36001 acgacaagca gttgaaatga cgaacttagc ttcgcatatt ttagaccagc tctctggcac
    36061 ttataataat ttgctgaata acaacttgaa tgacacgatg aaatttttga cggtctggtc
    36121 gctgatcttg accgttccaa ctattgtgac aggattcttc ggaatgaatc tccagttacc
    36181 ctttacccat tccgttttcg gatggggaat cgccttgatc atcagtttgg tcttatctat
    36241 ttggatgctt attgcactgt ggcggcggat tcgttgatct agtctttgat ctgcaaacca
    36301 agggcttggg cgatcacaag tgcttgctct tggttgacga ttagtcccgc caaagaatct
    36361 tgagataaag cgattgctgt gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt
    36421 cgaaaccagt tcgaaccggt taactgattg tcagacaaaa aaaggttttt ccaagtgacc
    36481 tcataaaatt cgctgtcttc taattgacaa tgatcgaagg agacagcttt caagtttgta
    36541 ctactaaagg aagccatcga agcaatacaa tcagtgaaag tacagtctcg aagataactt
    36601 tcggcaaaat tcgtacctgt taatttacat tgatgaaaca cgacttggtg gaaactgcca
    36661 ccaatccact ccagattgga aaaatcacat ttttcaaaaa tgacattact gcattcaaaa
    36721 tgacgtaaaa ctgttttttg catcgtaagt ttttggatat gactctctct taataccata
    36781 tttttggcat cgagatagct ttggtcaccc ccgttgatca gaagaccaga gaagatctcc
    36841 tcatcttcta aataaaactc tccctttgta agaagaggaa gagaaggtgg aataggttcg
    36901 atttttttca tgtgggttca cctcgttttt ctttagtata gcaaagaagc gggaatcact
    36961 gctagaaagt ctgctaatct aatcattttc agaaaaggaa gacaaactgt atgaaaaaca
    37021 ttgacaaaaa gattagtttt tgctatacta tgatggttga gaaataaaag cagaagcacc
    37081 cgcttctcgc cttagttgat aatgtcactg ggctagatag ttttcactgg ttctattagg
    37141 tgaagtacgc gcgggttctg taagagaact cgttttttta ttgccgttta ttcggtaagc
    37201 ttttctttct ctcataatat ttggaggtga atgaccatag caaaggatat gatggttaac
    37261 gacggcattc gtgcacgaga gttacgattg atcggttcag atggtgagca attaggagtt
    37321 aagacaaaag cagaagcatt gaatattgca gaacaagcaa acttggatct tgtgttagtt
    37381 gcccctggtg cgaaaccacc agttgcgcga atcatggact acggtaaata ccgtttcgaa
    37441 caacaaaaga aagaccgcga agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt
    37501 cgcttaagtc ctacgatcga cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag
    37561 ttcttggaaa agggcgataa agtaaaagct tctatccgtt tcaaaggccg tgccattacc
    37621 cataaagaaa ttggtcaaaa agtcttgaat cgcttagctg aagaaactgc ggatattgca
    37681 acagtggaac aaaaagcgaa aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa
    37741 aacgacagta agtaatttga atgactaact ggctgttgga cagccgatag tcggataacg
    37801 aaagattttg aggaggaaat atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa
    37861 aacgtttcaa acgtactggt aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc
    37921 gtttccacgg caaaacaaaa aaacaacgcc gtcaattgcg taaagcaagc atggtttcta
    37981 gtggcgactt caaacgtatt cgccaacaac tagcaaaaat gaaataagaa gtacaaataa
    38041 cagaatctga agatttattt ttattaggag gaattataca tggcacgtgt taaaggtgga
    38101 acagtaactc gtaaacgtcg taaaagagtg ctaaaattag caaaaggtta ctacggttca
    38161 aaacacactt tatttaaaac agcaaaagaa caagtaatga attcatacaa ctacgcatat
    38221 cgcgatcgtc gtcaaaagaa acgcgacttc cgtaaattgt ggattgctcg tatcaacgca
    38281 gcagctcgta tgaatggctt gagctactca aaattaatgc acggtttgaa agttgctgaa
    38341 atcgacatca accgtaaaat gttagctgac ttggctgtta acgatgcagc agcatttact
    38401 gcattggctg accaagcaaa agacgctttg gctaaataag ctaaacaatt ataaagagga
    38461 aagaagataa cgaaaagtta tgttctttcc tcttttttta tttacaatcc tccatcaaat
    38521 gaaacaggac aataacgtat aagtctaaaa tagcataatt aaggtttttt aattgaaatc
    38581 cgaacattta tggtaatcct ttaacgaaag cgctaactgc ataagttaga atgagtttaa
    38641 ctgatatatt agtttgttta tcagaaaaac aaaatggacg gaggaataac atgagtgata
    38701 ttattattgg tattttgtta gttatctcat tctttttcat ggtctggtac tgtgtaaaag
    38761 gctacaactt gatggtcggc tttgccatca tggctactgt atggatggga ttagcgctag
    38821 tcggaaacac gttttcacca aacccggcga tggaagggca aggagtaatt gatgtgttga
    38881 cgcatatcta tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct
    38941 ttggtgcctt tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa
    39001 aggtagttga acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa
    39061 ccgctgttat attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg
    39121 tgttgccgat cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg
    39181 gctcgattat ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt
    39241 atgctggttt caatccagcg gctgaaagct atacttataa tgactatttc caaattggta
    39301 tgattgggat gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca
    39361 aaaagaaacg ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga
    39421 tctcatggtt agccgtatta ttaccagttt taggggtcgt tcttttagat cttccaatca
    39481 tcttaggttt tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag
    39541 gttataaaga aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg
    39601 caccaatggt tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt
    39661 ctccttattt ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa
    39721 tcgtatttgc tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt
    39781 caggcttagc tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt
    39841 tgttcccgtt atttgcaatc acaacgattg ctcctcagca tttagatatt acacaatctt
    39901 gggttgcttg gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca
    39961 tcccaactgg ttggatcatc ggagcgatct tatgcctgat cacgttttta ttatacggga
    40021 acgcctagta aacgaaacgt cgataaaaac gattagatga atggaggaaa agaaaatgaa
    40081 acgttttgtg cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga
    40141 taatgcgacg cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg
    40201 tggagtagcc gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag
    40261 tccagaagat gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga
    40321 aggagacgta gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt
    40381 agcaaaacga caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat
    40441 tgtcaatgca ttcttacctg tcaaacactt aaatgtagac cgtgtttcag aaacgatttc
    40501 ttctttagaa agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa
    40561 tggggaacca gaacgagttg tttatgaagc agcatcggta aaatcgatcc ctacaacgat
    40621 ggcttctgac atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa
    40681 tgcatcaatt ttaccaaaaa tgctagatac ggcaacctct acagaagatt ccgtacgaga
    40741 agcaggggtc aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa
    40801 tgaaatgaaa aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg
    40861 ctctttgatg tacttgagag cgtctaatgg tgtttatttt gaagtaggtg gaacaacaac
    40921 caatatcggg gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca
    40981 cccaacatat atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt
    41041 acgggctaat cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct
    41101 agattatgca gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc
    41161 tcctaaagaa ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt
    41221 gactattaca aatacatgtg cagccaatgt ccctggatta gtgcaagaag aacatttttc
    41281 ttatggaaat gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac
    41341 gacggttgaa gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt
    41401 gattttagaa ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt
    41461 agggggcggc gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc
    41521 gattcctgaa aacgctgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga
    41581 tgttgtagaa cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga
    41641 agcgatgaat aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga
    41701 aatcgatcca cagacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc
    41761 aaccgatcta accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat
    41821 gcgattaaat aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg
    41881 cgaacagaat cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat
    41941 caaggtccaa cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc
    42001 tgtcgaacaa ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga
    42061 attttatctc tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact
    42121 acagcttttg atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt
    42181 tgccggaaat atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc
    42241 ccgaagaaat ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt
    42301 tgtccaagca gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta
    42361 gcatcaaaaa aaagtaccct tttgccgatg taggagaaat ttgtgaacaa atggctgttc
    42421 caatcgtttc ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag
    42481 aagatgaagg gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag
    42541 cttcgatctc taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca
    42601 tcgaagcttc tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc
    42661 ctttttatac acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt
    42721 caaaagagat gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc
    42781 catataatga aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat
    42841 gtacggagta tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc
    42901 ttttttccga ataaacggtg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa
    42961 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct
    43021 cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    43081 ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt
    43141 tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa
    43201 aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc
    43261 tgattctaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta
    43321 attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa
    43381 tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa
    43441 tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac ggttcaagtg
    43501 cgtactataa ctttctagcc aatcccaaat ttctcgtttt ccagcaattt cataacaagt
    43561 tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta ttttgagcga tcactgcctg
    43621 atcggtttgt tctagtattt tttctagttt agcttgatct tgcttggtca ttttcgcact
    43681 gcattcttgc aggatcggtt tttccaactt ttcccgcgta taacgggcta atgaagcaga
    43741 tcggatatcg atttttgaaa cataagtgcc ggactgtgga atggttttta caagctcttg
    43801 attgcgcagt tggatcagtg cttctcgaat cggtgtacgc ccgatagcta gttgttcttc
    43861 taacacttta tcggagattt tacttccagg tgctaactcg gaataaatga tttgtttacg
    43921 tatagattca taagcttgct gttgcaaatt attgataacc atttaacctt cctttctatg
    43981 cagatataat ctattataac ataaatcgct tacaaatcgt gtgggcataa aaacacagtc
    44041 tgcaaaaaaa gccctgatag gcatgcctat tagagcctcg aaaagaaaaa tattgaatca
    44101 atctggtcgt acctcttgga tcggtttgac acgaccgccg attcttttag caaaagcttc
    44161 ggattctttt ttgtctttga aaatgagcag attgtctaag tttttttccc gagtgcgaca
    44221 gccagtttta tcgatgtaac cgtgcattac tttaacgaca tacatgggga tcacctgctt
    44281 ttttcttttc attatacgaa ctactcgaca caatacaaga aaaatgctta gacggtcttt
    44341 attaagaatt tacatcatcc aaagaagcgt acgcttggaa taactgatct aactccggat
    44401 tgactttgct cagcttcact aatcgctgcg aatcagaaag taaaaaacag tgtttactac
    44461 tgccagtagt ttttaatttt ttgtcttcag ttcccacaat ttcgtagcta aaatccaacc
    44521 gtgtccccgt atacttttgg atagttggat ggatgctcac tttttctccg taatggatca
    44581 tttctttata gctgcaattt acttccagaa cggggatgat gataccagat tcttcgattt
    44641 ttttataggg aaaccctaga tgtaccaata aggctacccg agcctcttca aaccagcgga
    44701 tataattgct gtgatgaata atccccattt gatctgtttc ataataaaaa ggttctcgca
    44761 cataacctgt aaatttctcc atgactcatg cctcgttttc cattgatttt ttacaaaaat
    44821 aattcatcca cttcggaagt gcttcgctga tttgtgtcgg caaaacaata tattgttctt
    44881 ttgccagctg gtctccaatg aaactatgaa gataaacagc tgctgcaatc gtttggtcat
    44941 tttgaggaaa ctgggctaaa aatcctgtaa tcatcccagc taaagtgtca cccatgccgc
    45001 ctgttgccat agcagcagaa cctaaaggat tggtgtagaa gtgctggtga ccataaattt
    45061 ttgtctcatg acttttcaaa acgagcacag caccgatttc ctgttgcttc tttttgtttt
    45121 ctttatcccg ttgttcttgt atcggtagag aactcaaccg ttgccactcc atttgatgag
    45181 gtgtaagtac agtttgttcg ggatagggta gcaaaagatg attagcagca agtaaactta
    45241 aagcagagcc atcaatcacc agccattgtt cttttgattg gttttctagt acttgtttga
    45301 aacgttccaa actcttagaa gaagtaccca atccgggacc aatcaagagt acatctgctt
    45361 gctttattac ggaaagaaac tggtcagtat ccgtccaatc tacaatcatt gcttctggca
    45421 gatgactgtg aagagcactc cgattttttt catcagttac tacagtcgtg agacccgctc
    45481 cgctgtgtac ggcagctaaa gcactcataa tgattgctcc gccatattgt tcattgccgc
    45541 caatcaaaac gatacgtcca aatgttcctt tatgtgaaat ttttggacga ggtgtgatga
    45601 ctcttttcac tagttgttcg tttatcagca tcgaaaaacc tccttttact cattatagcg
    45661 tatatttttt ttagagaaaa acagataaga aaaggaattg tcagattttt tgaactattt
    45721 ttttctgtaa tttgtggtat catgaacacg aagacttaaa taagtggagg attaacttta
    45781 tgacaattga ttggcaaaaa gaagtagaag cacgtaaaga agacttgctt gaagacttga
    45841 aaaatctttt acgtgtcaac agcgagcgcg atgactcaaa agtaacacca gatgcaccat
    45901 ttggacctgg acctcgcgac gcattgaaac atatgttagc ttatggcgaa cgtgatggat
    45961 ttaaagtaaa aaatgtcgat aactatgcag ggcatattga tcttggcgaa ggagacgaaa
    46021 cgctagggat atttggacac atggatgtcg ttcctgcagg agatggctgg gacacagatc
    46081 cttatgaacc agtaattaag gatggaaaga tctttgcacg cggatcaagc gatgacaaag
    46141 gcccaagtat ggcagcttac tatgcgatga agatcattaa agaacttgac ttgaaactat
    46201 caaaaaaagt tcgcttcgta gtcggcagcg atgaagaaag cggctggggc gatatggctt
    46261 attatttcga acatgaagaa gagccagatt ttggtttctc accagatgct gaatttccga
    46321 tcatcaatgg tgaaaaagga aatgtttctt tagctttacg attcaaaggc gataatgcag
    46381 gagattatgt cttgaaatct tttgtttccg gacttcgcga aaatatggtg ccaggaacag
    46441 caactgcagc acttcaagta ccaagtgcag atgcagctat cgcgatggaa gaagcatttt
    46501 accaattcat cgaagcaaat ccagtaagcg gaacgattga agcagacaat acgtatgtaa
    46561 aaatcgaatt agttggtaaa ggcgcacatg gtgcaagccc acaatctggt atcaatgcag
    46621 gttctttctt ggcattgttc ttagataact atgaattcct aggatctgct aaacaattta
    46681 tccatgtagc agctgcttat gtccatgaag atttttatgg agaaaaacta ggagtcgctt
    46741 atgaagatga aaaaatgggc aaattgacaa tgaatgctgg actatttgct tttgaagaaa
    46801 atggtacaga ggaagcgaac ttcatcaata tgaatttccg ttttccaaaa ggtgtaacag
    46861 tagacggatt gcaaagtgac atcgaacaaa cagtggggca agaaggcgca acagttactc
    46921 gaggtgctcg cgtaatggaa ccacattacg taccaatgga tgatccattg gtggctacat
    46981 tattgcaagt ctatgaagac cacactggag aaaaaggcta cgaacaaatc atcggtggcg
    47041 gaacttacgg acgtttgttg aaacgtggcg ttgcttatgg agcgatgttc ccaggatata
    47101 ctgatacaat gcatcaagca aacgaattta tgagtcttga tgacttgttc agagcaacag
    47161 caatctatgc agatgccatc tatcgtttgg cgaaataatt aattaaaaag aggatcaaga
    47221 attgcaaatt atttttgcag tcttgatcct ttttttactt tctactgtgg tattcatgat
    47281 aaacatgata tagattcaat tcatattctt tcttagcgtt cgtgacgact gtatccaaaa
    47341 tcaaccctgt tactaatgaa agaagtgcta aagtcatcaa tccagtactt aaaatcgcag
    47401 aagggatgcg tgtaataaag cttgtcagca tatattcgcg aatcacagga atccccgtga
    47461 ccaatccaaa taaaaagaag aagatggtcc aaatcccaaa gaaaagcaaa ggcttataat
    47521 ctttaaacat tttaataatc atcatgatca ctttgaatcc gtcagagaat gtattcaatt
    47581 ttgattcgct gccttcagga cgatcacgat aatcgattgg gatttcaacg aacttgaatt
    47641 ttttatctaa agcatggatc gtcaattccg tttcgatttg aaaaccagaa ctcataatag
    47701 gaaaactttt gacaaagata cgattgaatc ctcgatagcc ggtcatgaca tcaagaattt
    47761 ttgttttata cattctagta attgtatttc taacaaggtt atttccaaaa tcgtggaaag
    47821 gtctcttatt ctcatcaaag taggttccat ttgataggcg atcaccaatg accatatctg
    47881 cttcaccaga acgtaatttc tcgagcaaac catgtacagc ttctgcagga taagtatcat
    47941 cgccatctac cattaaataa tagtcggcat cgatatcaaa gaacatttgg cggatcacat
    48001 ttccttttcc ttgacgaggt tcttttttta cgattgctcc accttgaacg gccaattcgt
    48061 atgttcgatc ggtcgaatta ttatcataga cataaatatc tgcttctggt aattctcgtt
    48121 taaagtctgc aatgactgtt gaaattgttg cttcctcatt gtaacaagga atcagaacag
    48181 caattttttc ttgttgttcc atttttcact ctccttaact atagaaaaca aaagctaaat
    48241 taaaaatacc tcataatcaa aggaaatgct agtttttccc gctaaatatc atttatttaa
    48301 aaagaaaata ctggtatata tgtttggaaa gctctatcta aaaaaccacc cactacaaaa
    48361 gaaggacatg atacctctct tttgaatagt gggtgcgtat tcgttctctc acttttttga
    48421 cgtattaatc tgttgcacct ttttttcttg aaatttctaa tacggttcct gtatgagcat
    48481 ctgctaaaaa ttcataataa acagtttcgc catcttctat tcgagaaaga cctccccgat
    48541 aagcactcga ccgaacggcg aatttcctaa taggttcctt ttcaaaagag atccaagaac
    48601 cttcgattgc accttctttt agaaaagatg acttgatatt ttcaagtact tggtctgctg
    48661 acaatacttt tttgcgatga taccaaatcg ttgaagctac cccgctcaat aatcctagac
    48721 cgattccaac tgctaacccg cctttaaaat agcttagttc gctttcttca tacatgtgga
    48781 catacctcct tcttctcact tgttactcct tgtgcttatt ttaccacact ctcatatttt
    48841 ctttgaaata aataaccgct accagaaaaa ataagctata ataaatacga tcggtctgca
    48901 gaaaaaatca tattttatcc gcagatcaat ataagtctga gaggagttta gtctgaaatt
    48961 tagacgaata gcatatggaa gaaaaaacat ttcaacgaat caaagagtta acagaattac
    49021 aaggaacaag cggttttgaa caagatgttc gcgcttatat ggaaagtcat atggaaccgc
    49081 tggtagatga gttacagtta gatggtttgg gcggcatttt tggtctgcgc catcatgaag
    49141 aagcggatgc accccgtgta atggtcgcag cacatatgga tgaagtcgga ttcatgctga
    49201 cacaaatcca agaaaacggg ctttttaaag tcgtacctct aggcggctgg aatccttacg
    49261 ttgtttctgc acagcgtttt acacttaaaa cctctactgg taagaattat ccatgtattt
    49321 cttcttccgt tcccccgcat ttattgcgag gaacaagcgg acaaaaatca gtagaagtaa
    49381 ccgatatttt gttcgatgct gggtttgaat caagagaaga ggcaatgagt tacggtgtcc
    49441 tcccaggaga cacaatcgtt ccttacgctg aaacgatcaa aacagccaac ggaaagaata
    49501 tcatcagcaa atcatgggac aaccgctatg gctgtacaat ggtattagaa gctttagaag
    49561 cgttgcaaaa tgaaacatta ggtcatacat tgattgcagg cgcgaatgtc caggaagaag
    49621 taggcttgcg tggttctaaa gcttctgtca ataaatttaa gccagatctg ttctttgcag
    49681 tagactgctc tgcagcagat gatacagtga cgaaaaatgg cacattcgga catttaggag
    49741 aaggtacatt gatgcgtatc caagatccag gtttgatcat gcttcctcgt ttgcgcgagt
    49801 acttgctgga tatcgctgaa acaaacaata tcccttatca atactttgtt tcaaaaggcg
    49861 gtacagatgc aggtgctgct catacacaaa acgaaggaat cccaagtaca gtgattggcg
    49921 tagtgggacg ttatatccat acgcatcaaa cgatgttcag tattcgcgat tttgaagctg
    49981 cacgagaaat gttgattcaa acactgaaag gtttggataa atcaacagtg aatacgatcg
    50041 tatacggaaa ataatgcgat agtgaaacta taaaatctgg caaaagattg cttttgccag
    50101 atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg tataatttaa
    50161 ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa tagtttagaa
    50221 gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc cggctggtgc
    50281 ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt ttctgattgg
    50341 caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg gaacattttt
    50401 ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct agtgaataaa
    50461 gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacgaa aggctgacat
    50521 ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg tatcgacggt
    50581 agtgaacaag cccttgctgc tttcaaaaaa gcggtagaag tggctcgccg taataatggg
    50641 aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg ctatgcacct
    50701 attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat cgaagaatgt
    50761 aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc atatggttct
    50821 gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct aatcatggtt
    50881 gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc cagctacgtg
    50941 atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca gccaagtaat
    51001 taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta aagggactgt
    51061 gacaaaattc ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaggcttctt
    51121 cggaaataaa gcatgaaaaa aatctcatgc agtgaatgca attttttgtt aagataaatc
    51181 tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga
    51241 gacaccttga tggttatcat agccgacgat aaaggaacag aaaacaccgt tgaacgcaaa
    51301 tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat
    51361 atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga tgaggaatta
    51421 gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttgg agctgatcat
    51481 gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga ttctgaccat
    51541 ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt ttgcggtgca
    51601 cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat gatgccagat
    51661 ggaatgatga tctggtcagg aactttgcga ggggtagata gctatggaat gatctgttcc
    51721 gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt attgcctgag
    51781 gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa
    51841 cctctttttt attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc
    51901 aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct
    51961 cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca
    52021 tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg tgtagctgtt
    52081 tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct
    52141 tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc
    52201 acttttccgt ctttttccag ttgattgatg atattcacta catcgttact tggaatagca
    52261 aatcccattc cttctacgct tacgttagaa gtagattcag aagtacttgc gatcttactt
    52321 gagttgatac cgataacttg tccttcaata ttcactaaag gaccaccaga atttccaggg
    52381 ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga ttcatttgta
    52441 cttgttactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt cgcatattca
    52501 gatcctaatg gggaaccaat cgcaatcgca ggttcaccaa ccttcagagc acttgagtca
    52561 ccaaaagagg caaccgtttc gactttatct gaattgatct tcaacaccgc taaatctgaa
    52621 taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat caagacttct
    52681 agaccttgct gtccatctac tacgtggttg tttgtgacta cataagcagt atttccatct
    52741 tttttgtaga tcacgccact tccttcacta taagcttcca aattactatc atctgagctg
    52801 ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt ttggctttgt
    52861 aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa
    52921 tctacattga ctttgacgtt ttccacaact gtttcaccag cagaattttg attgccactt
    52981 gtagtagaac tattgttgcc agtgcccatc gctgcataaa aaatgccagc tgtcagtagt
    53041 cctccaacga tccctccaac aagccccaga cctaacttac gccagaggct attattttta
    53101 ttttttttca tctttggtgt cacatttttt ctatccatgt gttcctcctc caattacatt
    53161 tatttatcta ctgttagtat agtcttattt gttgacttta gtctactttg agactgtgaa
    53221 ctttttatga aaaactttag gaaaaatcat tgttttcaga tgaaaatatt tgattttctt
    53281 tttccacaga tactgtgtaa aactacgcgg ataggattgg aaaaataagg gataaaacat
    53341 atccacagcc tgtgtataaa gtggataaat tcttataaaa cgtatgtttg cctaatgaat
    53401 gtgatgaaat aaggtaaaat aacactgttt tctgtgtata aacctgtgga tgttgtgcat
    53461 aactgttggg gaaatctttt taacaaaaat agccaaaata aacatataga cgaaaaaatt
    53521 gattataata aacaaagtga gcgtgaagac caaaggagag aaaacatgaa tatcaaaatt
    53581 atctcagtag gaaaattaaa agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa
    53641 cggctgaatg cctatgcaaa aatcgaattg atcgaagttc ctgacgaaaa agccccggag
    53701 aatttaagcg aagcgcaaat gagacaagta aaagaaaaag aaggggaacg gatcttagcg
    53761 aaaatcaaag aacaagaata cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt
    53821 gaatcttttg ctaaacaaat cgatcaatta ggtatccaag gaaaaagcca ccttgttttt
    53881 gttatcggcg gctctttagg actcagcgaa gccgtcatga aaagaagcaa tgctcagatc
    53941 tcttttggaa aaatgacgta tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc
    54001 taccgtgcct tccggatcaa tgcgggggct ccatatcata aataaggata gttgtatcaa
    54061 aaatattctc cacctcgcac cttttttagg gatgtcagtg ggtcaatata cgcggagtgg
    54121 ttgccaactt cagaatatga gttggttcaa gcatcagaac tctcctttac tgatttctca
    54181 aaagagttga cggattgtga tggcgaaatt tggattgcga taaaaaagaa gtaaatagta
    54241 ataaaagata gcgtaaattt agggagctca gtcatctaat cgatggttgg gctatttttt
    54301 tgtgggaaat ttctcttaat aaagaaacta tttcctaaga taacgactgt ttccctgata
    54361 cttctaaaac tattgttaaa gtaacaaggc gtagatatac tttgggatgt aaacgataac
    54421 aaaaaaggag acgatagata tgggattagt attagcaaga atcgatcaac gattgattca
    54481 cggtattgtg gtaacacagt gggcaggtgc gacaaaagca aaacgattga tggtcgtaga
    54541 tgatcaagtc agtaaggatg acacacaaaa agcagcaatg cgaatgagta agccagctgg
    54601 aacggggatg tcaattattg atacagatac agctattatt aatttcaaac ttggcaagta
    54661 tgacagtcat aatgtgctta tgatcgtaaa agaaccgagt acattagtaa aactagctga
    54721 gaacggtatc gatataccaa aagtgaatat cgggatcatg tttgatggtg aagggaaaca
    54781 aacagtaaaa aaaatggttt cagttgatga acaagaaatt gctgatttaa aaaaattaga
    54841 ggctatggga ataccagtta cttttcattt tgttccttca gatcctgaag aaccattaga
    54901 tgtgagaata aaataaagga gataattgga atggaaatta tccaaggaat tttaattatt
    54961 attttagcag tgtgggttgt attagatcaa caaggattgg tcattacaac atggtttccc
    55021 atcatgattg ctttgtttgc agggctgatt atgggagata tgacaaccgc aatggttatt
    55081 ggcggtacgt ttcaattaat ggctttgggt gtagcgaata ttgggggatc gtccgttcct
    55141 aactggggtt tagctgctct tgtaggtatc tacgtagcca tccgtaccac aaataatatt
    55201 gaagaagcaa aagctgttgc attagcagta ggtgttcctg ttgggatgct gggaattcaa
    55261 ttagatgtgt tggctaaaat attgaacaca tatgttactc atgcggcaca aaaggcagca
    55321 aataaaatgg attttaagaa aatgaatcag attttgtggt taggaccagt gattttcgga
    55381 ttatctacag ccattccaac tgcattgtgt gttgtttttg gtgatagaat tgttcgattg
    55441 attttagatt ttgtgcctaa gtggtttaca gatggattat cgattgctgg aagcatgttg
    55501 cctgtagtcg gaatagcatt acttctacaa gttatgccgg tgaaaaaata tatgacgatg
    55561 ctcttgattg gttttgtact ttccgcctat ttaaacatgc ctattttggg aatttcaatc
    55621 gtcggctttg ctttagcgta ttactttttc acgacagaaa taaaaaaagc agagacggca
    55681 tcacctgtag ctgccaatat agtcgatgaa ggagatgatt ttgatgaata atgaaggaaa
    55741 taaattatca aagaaagata tacgtagagc agcatttcgc tatatgttta tggcatgtaa
    55801 tatcttcaat tacgaaaatc aacaaggtcc ggcagttgtt tatggacttg aaaaagcact
    55861 aagaaaaatt tatccaaatg atgaagacta cagagcttct ctgaataacc attttaaata
    55921 tttcaataca acgacttgga tggcgaattt attattaggt gccagcttag caatggaaga
    55981 aaaagatgga gtcagttcat tagatgcagt tcaaagtttt aagactggga tgatgggtcc
    56041 tttagctggt gtgggagaca cgttggtttg ggtcctttat ccaacgatta tgggctcgat
    56101 tgcagcttat atgggtctag aaggaaatcc gactggtgcc attatttggt tatgtttgaa
    56161 tctattcttt ttgttcttcc gatttaaact tttttcgatt gggtatactt cagggttaaa
    56221 gttggtgact gctttaggaa gtaaattatc gatatttaca gaatctgctt caattatggg
    56281 tttgacagtc gttggagctt tgattccatc tgttgtaaaa atgaatgttg cagtggtttt
    56341 tcaatctgga aaagtaaaat tacctattca aacagaaatt cttgataaaa tcatgccagc
    56401 actcttacct gcagttttaa caattattgt ttataaattg atagcttcaa aaaaaatgac
    56461 ggtcattcaa attatttttt cagtgatcat attcgcattg attctatcat ttttcggtat
    56521 tttaaaggtt taaggagaat aaagaatgaa aaaaattatt tttgctagtc atcataattt
    56581 agctgtagga ttgaaagata ctgtccaata tattttaccg aatgttggtg aaataactgc
    56641 aatcgcaggt tatattgata actcatctat agaagaaaag attaacgaag taatggtaag
    56701 agacaatgag gatactgata tattgatttt tactgatatg ctaggtggtt cagtcaatca
    56761 agcatttata aaatatatca atcaacctaa tgtctatctg attaccggga tgaatctacc
    56821 tgtggtattg acaattttat tgaatacatt aaacaaaaga ttgacagaca accacatccg
    56881 tgaggcaatc caagaagcac aagaccaatt ggtttatgtc aatgattact taaaaaatat
    56941 agtcgttgat gaggaggatg agtaacggat gaatgaatgg tggaaaagta gtgttgtcta
    57001 ccaagtgtat cctcaaagtt tcaaggattc aaacaatgat ggtatcggag atcttatggg
    57061 gatcgttgaa aaattacctt atttagcaaa gttaggggtt gatgttatct ggctaactcc
    57121 aatttttgac tctccgctaa ttgataacgg atatgatatt cgagactatg agaaaatttc
    57181 agaaaattat ggaacgttag aagattttaa gagattattg gaacaggcgc atcaactgaa
    57241 tattcgagtt attttagacc ttgtcgtcaa tcatacaagt aatcagcacg cttggtttat
    57301 agaatcacaa aaaaataaag aaaatccata ttctgattat tatatttgga aagatcccaa
    57361 agaagatggg agtgcaccta cgaattgggg atcgacattt ggcgggtcgg cttgggagta
    57421 tgttgagagt agaaagcagt attacttaca ttgttttgca aaagaacaac cagatttaaa
    57481 ctgggagaac ccgaaagttc gtcagtctat ttataaaatg atgaaaaaat ggtttgatct
    57541 tggaattgat ggattccgaa tggatgttat cagcttgatt agcaagcatc ctgattatcc
    57601 tgatgctcca aatagcatgc cctatacaaa gtcttattat gtcggagcat ccaacggtcc
    57661 gcgagtgcat gaatttttac aagagatgaa tcgagaagtt ttgagtaaat atgacagctt
    57721 aactgtcggt gaaacaccga acacaaatag cagacaggca cagctttata cgtttcctga
    57781 gcgtaaagaa ttaaatatgg tttttcattt tgatcatatg catcttgatt atggaaagta
    57841 tggaaagttt tcagatgtaa ggtttaagct tagcgattta agagctacgt taagtgagtg
    57901 gcaagaagcg ctttcagatg gttggaattc tctttactgg agtaaccatg atcaaccgcg
    57961 agctgttact agatttggtg atgaaaacga atatcggata gagtctagta agatgttagg
    58021 aacactactt cacatgatga aaggaacccc atttattttt caaggggaag aaattggaat
    58081 gaaaaatgtt ccattttctt caattgatga gtatcaagac attgaaacaa actattttta
    58141 taaaaagatg aaagaagaag gcgagagtga tgagtacatc aaacgtgcta tttatttgaa
    58201 atctcgtgat aacgcacgaa caccaatgcc ttgggatcat tcaaaagggt atggattctc
    58261 gaacagtgat ccttggatct ctttttcgcc agataatcaa caaatcaatg tagaagatgc
    58321 tttaaaaaat ccaaattcag ttttttatca ttatcaaaaa ctgatttcac tgcgtaaaaa
    58381 ctatccaatc attcagcatg gtacgtataa attgatcaat gagaatgatt cagcagtgtt
    58441 tggttatata cgtgagtata aaaatgaact attagtggtg atctgttctt tttcaaaaga
    58501 gtctgttccc tatgaattac ctaaatcatt gcaaggtcga aaaggggttc ttcttctatc
    58561 aaattatgag caatcaccat ctgaattgga agataagttt atattacgac cttacgaagc
    58621 acttgtttat catatatagc aagcacgtaa aaactgtgtt agaatttggg caataagaaa
    58681 gaatttcgga taaaacagcg caagttttat ccgaaattcc ttctgtatat atgagaggtg
    58741 aggaaatgga ttttgaacaa aatgttcgac taaactttga tttgctctca gataatgaaa
    58801 aagatatggt tttttttatt cgtacgcata aagctcaagt agttcacatg tccattgtag
    58861 aattgggaga acaattattg tcttctaaaa gttctgtctt acgtttagca aaaaaattgg
    58921 gatttcgtgg gtattctgaa atgaaatatt ctcttgaaca gagcatcgtt caatcggtta
    58981 ttccaccgac agatcttatc gcactattga aagatgaaat caaccgaact tttcaattag
    59041 cggaccaaac aaatttccaa ccgatattag aacagctaaa agcggcaaaa acagtagttg
    59101 tctatgcgac aggctttacg caaaataact tttcaaaaga ctttgtaaac gatttgattt
    59161 tatcgggacg accagccatg ttaatttcag gtgaaacaaa ctttgaaatg ttaagccata
    59221 ctttgacaaa agatgatttt gtgatcgtta cgtctctaag cggtgagaca ccaagcatca
    59281 aatcaacaat caaaaatttg aatatgaacc gaattccact ttgcggagtt actgagctgg
    59341 gaaaaaattt cctcagcgag cattccgact ttttgttata ttatgagaca agagagcttc
    59401 caagcaattt aattgaagga agtcgttcaa tgattggatt aaatattttg ctatcaatac
    59461 tctctagaaa gtaccgtgaa tttattcttt ttgatgagta gcctccattt cgaaacaaag
    59521 gagcaagtgt tgaaaagttg ttagccaatc gtttcaagag tgagaacaag gaaatctatc
    59581 gaacattcat tgataaagtg acatttgata agctgaacaa aggaaatatt caactatatc
    59641 tgaaatttga tgaaaaaatc atcaactaat tgaatgagca atgctaaaca gtcgtatctg
    59701 acaaagatgc ggcttatttt ttagagctaa gccacttcga tctatgttgt aaattagcat
    59761 aggctgttac gcatgcaata acaccccctg gatattcgta atatctaggg ggaaagttat
    59821 gtaggtcctt atcataaata gggatattta ttaatgaata tttgaaatat aattgtatat
    59881 aagaattaaa aagagtgggg atagatatga aaaagatggg taagtgtatt ttatgtgaaa
    59941 aatacactga acttactaaa gaacatgtac cacctaaaaa atcagggaat actggaggta
    60001 aaaagaccag aactggtaat ttagatgatt tccttaagag cgattttaca aaaggtgatt
    60061 ttccgaaggg gattaaaagg aaacctcaag gaaatgttta ttatacgtta tgtagtaaat
    60121 gtaatagttt ttttggtagt gagtatgttg aggaatatat tagatttgca gaagacaata
    60181 aaaatttcct ttataacaat acaagtttaa agaatggtaa gtctgattta acacattcaa
    60241 taaagaaaat gaattcactt agagttgcaa aagcaattgt agcaatgttt tttagtttaa
    60301 atggggatga agattcaatg gataagccat tcttagattc ggtacgagaa tatcttagca
    60361 atcctaaaag tactttattt cctcatgaag actacaaaat tataatgaat tattatcttc
    60421 caataggtgc tccacagaat tctaaaatca aaattcctaa tgacattgta aatgatggtg
    60481 acactcacaa tgtaataaaa ggttttatgc cccttgatct atcaactggg agaaaaattc
    60541 gattttcaga aattcagtat gaaggaattg gttttactct tatagattta aaaaattcaa
    60601 cttttgaaat aaaagaagga ttagatttaa aaaatctcct ttgtgcagat gataaaatca
    60661 aaaaaataca attgtacaaa gtccctatat taacaccaac agtacttaca atgttggaga
    60721 tggaaaataa tattaattat attgactcag gttcagattt gctgattagg tggcaggtaa
    60781 taactaattt tcttatggaa ttaaaaaaat acagtaatac tcaaacgaag ttctcttgaa
    60841 gttataaaga atacaataaa tataaatttc ctttagacta tattaaaatc agcaatcgaa
    60901 ggaaaataaa cgaagaaaat ggtacgcaaa atctaactat tagagcaaaa acaggaagct
    60961 gctttggaag atagtcccga attgtttccg atgttaaaag atcgtttaaa tgaattgact
    61021 gaaaaacgaa gacagttaca aattcgagag aatgaaatct tagggatctt tcaacaacaa
    61081 ggggaaccga ttcaagtcaa agatgtccaa cagatattga ctagtttgga ttgctttctt
    61141 atccagagtg agaagaagca agttaaagct ttgtatcaca cctttattga gaaaatcacc
    61201 tttgatccta atcagaagta gatattgccg atcccgatga gattcacacc tactgtgatt
    61261 tgataattga atgagtaata tcaaatagcc gtatccaaac caaaggatat ggctattttt
    61321 gtgttaggga tatacgtcac gtagcttgtt atcaagaata agtttcttta tttttttgct
    61381 ttttctaaaa ctgagtatgc taaaataaga gggtatatag caactttaaa taagagatag
    61441 gagtgtatct ttttgggtgc agaattaaat cagaagttat ttagtgcggc agataatctc
    61501 cgtagtaaaa tggatgcgtc agaatataaa aattatttat tggggctgat tttctataaa
    61561 tatttatccg accgtttgtt ggaacaagtg gttcttttag cagatgaatc cttagaggaa
    61621 tatgacacag taagcaaaca aacgatgttg tatcgtgaat tactttcaga tgaagaatca
    61681 aaagaagatc tgattgcaac aatcgttgat attttggggt atgcgatttc gccagagtat
    61741 cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt tccaattgaa tgatctaaac
    61801 aaagcctttt tagcaagtca tatttatgga aaaatactgt cggcaaaatt cgaatagtgt
    61861 cgaggaggtg ctgtgatgga agaattaaaa gaacgcattg tcaattatgt catagagaac
    61921 tctgagatta aaaataaatg ggggaacaat acagcacaga aaatatctga aaaattaaat
    61981 gttagtcgta atattgtctc tcaatatttg aatgaattcg taaaagatgg agaatttgga
    62041 aaaatcaata ctcgtccagt aatcttcttt tctttaccgg agaaagttcc aaatcaacaa
    62101 atttttagct cttcagagga agcggaaaaa tggtatgata gctcttttag ggttggaata
    62161 gaaagggtta tcggaagtca aggaagtctg cgaaatgtaa ttgctcaaat taaagcagca
    62221 atctactatc cgccaaatgg cttacccatt ttacttcatg gcgagactgg aactggaaaa
    62281 agctatttag caaattatgt gtataaggat ttgttggcca ataattatat agaagagaag
    62341 gcatctttca tttcagttaa ttgtagtgaa tatgccaata atccagaact tttcttgtcc
    62401 aatttttttg ggcatgtaaa aggtgcatat acaggagccg aagatggaag aaagggttta
    62461 gcagaattag ctaataatgg agtcttattt ttagatgaaa ttcatgcatt gagcaatgaa
    62521 tgtcaggaga aactttttca gtttatggat acgaggaaat ttcatcggat gggtgataat
    62581 gaaaactggt acacatcaag ttgctggatt atatttgcaa catcagaacc acctgaggat
    62641 aaactattat caactttgtt gagaagaata cctgtaaagc taacacttcc aacattagac
    62701 agtcgttctc tttatgaaag aaaacagtta atcaagatat tttttaaatc agaagaagaa
    62761 agactagcaa agaaaatact gatatctcaa caattattag attttttatt aaactatcgc
    62821 tttagtgaaa atgttggcaa tttgaaaaat atcattcaat tgacctgtgc caatgccttt
    62881 tatttcttag atgatttgga agaactttat attcagatgt atcatttacc acaatgggtg
    62941 aatttcaacg gcatgaaggt tatgaaaaga cagaatgaat taaaatatat tcaaatggat
    63001 gatttggaaa aagtagacga agtttttaat atcaacgaac ttatagagta tctttatcaa
    63061 acgtggaaga aagacactga taagcagcag ctttttgaga aatatgatag atattttaga
    63121 caaaagaatg ttgatatcca aaataattca gctttaacat atcccaccta tcaagagatt
    63181 cgatttagac agtatgttaa ggaaatagag agagattttc aaatagaatt tgactattat
    63241 tatcttcaaa taacgtctat ctttttgaat tcttggattt atcaaatcca attaacatcg
    63301 gacgataaat tatattcctt catcaatgat attaatagtt ggtgtagtcg agaagttcaa
    63361 ttagtcaatc gttttattca aataggggat atttatttaa caaaaattca aaaaaatgtt
    63421 ttggttattt tatttaagtt aatgtgtagt aaagaatttc agcaaaaacg tttagcactg
    63481 attttggctc atggagatct aacggcaacc tgtatagcaa gtactgctaa taggatcgtt
    63541 gagagatata tatacgacgc cattgatatg ccactaatga cttcaagtag tgagattgca
    63601 gacaaaatta atagatactt acaagagtgt accggatatg aagaaattat tttacttgtt
    63661 gatatgggtt cactggagaa aatttatcta aatttggaac tagacgctca aaaaaaattc
    63721 ggactactaa acaatgtttc aacaagaatg gctattgatg ttgcaaatca gatattatta
    63781 gatttaccgc ttaaacagat tcttgaacaa gtaaaagaaa atcaaaatgt ggattatatt
    63841 tatgaatcat caaatattaa gaaggatatg attgtctgtt cttgtgcaag cggattagga
    63901 acttcgttta aattaaaaaa gatacttgaa gatagctttc caagggatac tgatcttgtc
    63961 atagaaacgt gtgactatta ctctttagtg aatgaaaact atttatcgga gcttcgtaaa
    64021 gagaacaata ttctttttat tgttggaaca ctaaatccac atctagaaga tgtagaattc
    64081 ttttcaattg aagatatgat tttaggaatg aatataagta aattctccaa tcaattgaat
    64141 agacacttta atgataatga actgaaagtt ttgaacaata atattctgaa gaattttagt
    64201 ctaacgaatt taatggagca attgaccata ttaaatccga ctaaattatt agaacgggtt
    64261 tcagatgcta tatacatctt acaaaatgat ctaggaattt catttgataa taatacgtgt
    64321 ttcggattat atgttcacat tagttgctta attgaacgat tagtaaaaca aaatactttg
    64381 gaagatgaaa tttatttcaa tgaaacgagt gaggaatttc aaaaatttca gacacatttt
    64441 aagcaatctt tcagtgtcgt tgaacattat tatagtgtcg acatcccgat tcatgaggtt
    64501 aaatacgttt atgattatgt aaaaagagct tgatttatca agttcttttt gttttggcat
    64561 ggtaattgct tgtaagaaaa tgaaagataa aggaggaagt atttatgagt attgttatgt
    64621 cgagagttga taaccgttta ttgcatggga ttattgtcac gcaatgggca ccaatgtcta
    64681 aagcaaacag agtgatggtg atcgatgatt ctgtcgcaaa taacgacatc caaaaatcaa
    64741 gtatgcgttt agctagacct gctggaatgg ctgtatcaat tatcactgaa gaaacagcga
    64801 ttgcgaattt taaaaagggg aaatacgatc aggaaaaagt atttgtcatc gtgaaagagc
    64861 cggaaacaat ccttcattta gtagataacg gtattagaat tgaccgacta attgttggcg
    64921 gtactgttgt taaagataat gcattacagc tatcttctag agcctatgca tcagatgaaa
    64981 atatcagcac gtataaaaaa ttgttggatt ctggtgtaga aattgttaca caatttgttc
    65041 cggcggataa ggcacaggaa attcaatcaa aattaggata aaggagtaag gataatgcat
    65101 tttactattt ttcaaattat tatgctgact atattagcat ttattaaaca tgttgattat
    65161 tatgggattc cgatgatctt tgtaaattac gcagtttttt ggggattatt cacaggaatt
    65221 ttgatgggtg atataaaaac aggtttgata gttggcggta cagttcaatt gatgtcatta
    65281 ggagttgccg gttttggtgg ctcatctgta ccggactatg gaacgtgcgc aattattgca
    65341 acggcatttg gtagccagct tggagtagaa gccggattag caataggact acctgtaggt
    65401 atgttagggg tgcaactaga tgtaattgtc aaaatcttaa atggttttgt tgttcgcaaa
    65461 tcacaggaat atgcgaataa acgagaattt aagaaaatga atagtgtttt gtggttgggg
    65521 ccaattttct ttggtttatc tgcagcaatt ccagtattta ttgccgttac tttaggacag
    65581 tctgctgtta attacgtttt ggatgtttta cctaattggt ttatggatgg gctaacatta
    65641 gccggaaaaa tgttgcctgc tgttggtatt gcgatgttgt tacgctttat gcctacaggt
    65701 cgatacatta gttatttact tgttggattt ttcttagccg cttttttgaa ggtacctatt
    65761 attggagcgg cgatcatagg attcgcttta gcatttaatg tgtatcatag agctgaaaaa
    65821 gatgcacaaa tcactgcttc tgcgggagct tctgatgttg ggtttattgg ggaggatgaa
    65881 taaaatgagc gaaaaatatc aagtaacgaa aaaggatctt aggaaagcga actatcgttg
    65941 gttgatgtca gtttgtacct ttaattatca aacacagcaa ggtgcatctg tgacttacgc
    66001 attgtcacct attttaagaa aattatacaa agatgatgat gaatacatcg aagctttaaa
    66061 taatcacttt cagtattaca atacacagcc ttggctggca gccattattt taggtgcttg
    66121 tgttgcaatg gaggaaaagc aaggattaga agcaaaagat gcgattaatg actttaaagt
    66181 tggtacgatg ggtccaattg ctggtattgg ggactctctt ttgatgacga tgatcccaac
    66241 catcatggga tcaattgcgg cttatatggc tttagaaaat aatcctgtag gaatcttttt
    66301 atggttcatt ttaatcatgg taatcttctt tttccgaatg agaggctttg aatttggtta
    66361 taagcaggga atgaagattg ttactgaata cggtgaaaag attaattatt taactgaagc
    66421 tgcttcagta ttggggctaa cagtagttgg atcattaatt gcttcagtta tcagtgtttc
    66481 aacgccactt aaattcactt ttggtgaagt tagtatgaaa atccaaccaa tgttggacaa
    66541 aattttacca acattaattc cagtaattat tactgctgca gcatattatc tactcagtaa
    66601 gaaaaaaatc agtatgacca ttttgattct actcgttatt gtttttgcga tggttatggc
    66661 tgcatttgga atattagcgt aaaggaaaag gatttgaatt atgagaagat tgatcattgc
    66721 tagccattca atactagctg agggttttaa agagacacta gagtttttaa caggtatgca
    66781 taatcaaatt agtagcttat gtgcatatgt cgaagaaatt gatgatatag atgaacaaat
    66841 taaagagtta gtggatacta cagacgaaat aattgtttta actgacatgg ctggaggaag
    66901 tgtaaatcaa aagtttttta cctatatttc tgacagagtg cacgtaattg caggaatgaa
    66961 tcttcccttg gcaatgtcaa tcgctttgca gttaggaaat gataatttag atattggagc
    67021 aattattgcg gaggcacaac aacaaatcat ctatatgaat aatcaaaaaa gtgaagaaag
    67081 gatagaggac gaataaaatg aattgtttaa ataatatatg gctccacaag ggagaggaaa
    67141 gtagaactat ttcaccagaa aataaaaatg gtgaaaaagg gaaagcggca atgacagcga
    67201 gtcatctagg aattgggaga aaagggtcac cttctgttcc gataaatgct ggagagacaa
    67261 ttactttagc agaaatggaa ggccctggaa ttttacgtca tttttggatg acagtgacag
    67321 acaaaacaat cgctgggagt ttcgtcttac gtgatttaat tttgcgtatt tattgggatg
    67381 atgaatccac tccttctgta gaaagtccat taggagattt tttttgtaat gggtttggga
    67441 caagatgcga agtgaattcc ttaccaatcg tagtgaatcc aacatcaggg atgaattgct
    67501 attttgagat gccatttcga aaaaaagcta agattacttt aacgaatgaa catcctgagt
    67561 ttattaagca cattttttat acatttaatt atgcattgac tgaagtaccg gatgatgcga
    67621 tgtattttca tgctaaatgg aaccgtgaac ccatcacgac aaaaggaaaa gattacaccc
    67681 ttattgatgg aatcaaaggc actgggtatt atgtaggaac ctactttgct ttgtgcgcat
    67741 tggaaagata ctggtggggg gaaggagaat ttaagtttta tatagatgga gatgaagatt
    67801 tccctacaat aacatctaca ggagcagaag attattttgg cggcgcttgg gctttccata
    67861 aaaagaactt tggagaaagg gcatatgctc aaaattttaa tacgctattt atgggctatc
    67921 cttttcaatc taaaagagat cacacccggg attattttag tgctggaaaa cctaatccaa
    67981 atccagttca tggttttggc gatgatgctt taccgatgca tgggttgtac cgctggcatt
    68041 tacccgaccc cattagtttc aaagaaaatt tacgtgttac tttccaaacc ctaggaaatg
    68101 atgacatcaa gctttatgag cgtcaagacg atatttcttc tgttgcctat tggtaccaaa
    68161 aggaaccaca tgagtttttc gaaaaactat tagacaggga agaacgaatt cctagataaa
    68221 aggacggtgg agtaagaaat gtatggctca atagaagcag gtgggactaa atttgtttgt
    68281 gccattggca atgatgattt aaaggtttta gaaagagtga gttttcctac aactacaccg
    68341 aatgaaacaa tgagtttggt aattgatttc tttaatcact ataaagaaca attagaaagc
    68401 attggagtag ggtcatttgg accgattgat atccatcgtg aatcaaagac ctatggacat
    68461 atcacctcta ctccaaaaac tgcctggaaa aattttgatt ttgttggaac gttgaataag
    68521 catttcgaaa ttccaattgc ctggacaacc gatgtgaatg ctgcttgtta cggggagtat
    68581 gtttcaggtc atggaaaagg tttatccagt gttgtttatt acactgttgg aactggaatt
    68641 ggtggaggag ctatacaaga tgggattttt gttgaagggt ttagccatcc agaaatgggg
    68701 catacgttag ttaagagaca ttcaggagat actttctctg gtacttgtcc ttttcatcat
    68761 gattgtctcg aagggttggc atctggaccc gctatcgaga tgcgtacggg aaccaaggga
    68821 caagatcttt ctatagaaga tccgttctgg gaaatcgagg cggattatat tgcacaatgt
    68881 gcctgtaata cgaccctgat gctttcacca gatattataa tttttggtgg tggtgtcatg
    68941 caacaagaac atctgaaaaa gaaagtacaa agacgttttt tagaactaat taacggctat
    69001 gtcgatacgc caaatatcga agaatacatt gtcacaccta agttagcaga taatgcagga
    69061 actataggat gcttaacttt agcaaaagat gttagaatca attcttaagc aaaaaagtat
    69121 tagtcaataa aatgcttatg aagtccgatc aacttaatgt ggtcggacta tttgtattca
    69181 gcaatatcat tcactcatga tacccagctc cataccacaa ataatgatat tttttaagag
    69241 aaaaattatc c
//
LOCUS       DACERL010000005        67213 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.005, whole genome shotgun sequence.
ACCESSION   DACERL010000005 DACERL010000000
VERSION     DACERL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67213
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.005"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..406
                     /locus_tag="IXF54_000320"
     CDS             <1..406
                     /locus_tag="IXF54_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HAQ1403605.1"
                     /translation="IIENMSFSKNLSEAAELSLEGKIVQDADRIEALGAIGILRTAYF
                     GGGHGHPIFDPELYPQTFKDKENYRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRE
                     TFMREFLEEFFLEWSIDDENFTPDNWIKNNKK"
     gene            1051..2241
                     /locus_tag="IXF54_000321"
     CDS             1051..2241
                     /locus_tag="IXF54_000321"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1403606.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            2343..3296
                     /locus_tag="IXF54_000322"
     CDS             2343..3296
                     /locus_tag="IXF54_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1403607.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            3365..4714
                     /locus_tag="IXF54_000323"
     CDS             3365..4714
                     /locus_tag="IXF54_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1403608.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4897..5220)
                     /locus_tag="IXF54_000324"
     CDS             complement(4897..5220)
                     /locus_tag="IXF54_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403609.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            5349..6512
                     /locus_tag="IXF54_000325"
     CDS             5349..6512
                     /locus_tag="IXF54_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403610.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            6582..7931
                     /locus_tag="IXF54_000326"
     CDS             6582..7931
                     /locus_tag="IXF54_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1403611.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7976..8407)
                     /locus_tag="IXF54_000327"
     CDS             complement(7976..8407)
                     /locus_tag="IXF54_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403612.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8795..11437
                     /gene="alaS"
                     /locus_tag="IXF54_000328"
     CDS             8795..11437
                     /gene="alaS"
                     /locus_tag="IXF54_000328"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1403613.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11637..12233
                     /locus_tag="IXF54_000329"
     CDS             11637..12233
                     /locus_tag="IXF54_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1403614.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            12355..13503
                     /locus_tag="IXF54_000330"
     CDS             12355..13503
                     /locus_tag="IXF54_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1403615.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(13541..13954)
                     /locus_tag="IXF54_000331"
     CDS             complement(13541..13954)
                     /locus_tag="IXF54_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1403616.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            14033..14464
                     /locus_tag="IXF54_000332"
     CDS             14033..14464
                     /locus_tag="IXF54_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1403617.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(14563..14796)
                     /locus_tag="IXF54_000333"
     CDS             complement(14563..14796)
                     /locus_tag="IXF54_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1403618.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14979..16649
                     /locus_tag="IXF54_000334"
     CDS             14979..16649
                     /locus_tag="IXF54_000334"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1403619.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16796..17182
                     /locus_tag="IXF54_000335"
     CDS             16796..17182
                     /locus_tag="IXF54_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403620.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            17169..17867
                     /locus_tag="IXF54_000336"
     CDS             17169..17867
                     /locus_tag="IXF54_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1403621.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17872..18336
                     /locus_tag="IXF54_000337"
     CDS             17872..18336
                     /locus_tag="IXF54_000337"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1403622.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            18333..19244
                     /locus_tag="IXF54_000338"
     CDS             18333..19244
                     /locus_tag="IXF54_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1403623.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            19479..20018
                     /gene="pyrR"
                     /locus_tag="IXF54_000339"
     CDS             19479..20018
                     /gene="pyrR"
                     /locus_tag="IXF54_000339"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1403624.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            20032..21315
                     /locus_tag="IXF54_000340"
     CDS             20032..21315
                     /locus_tag="IXF54_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1403625.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            21328..22254
                     /locus_tag="IXF54_000341"
     CDS             21328..22254
                     /locus_tag="IXF54_000341"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1403626.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            22273..23553
                     /locus_tag="IXF54_000342"
     CDS             22273..23553
                     /locus_tag="IXF54_000342"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1403627.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            23558..24634
                     /gene="carA"
                     /locus_tag="IXF54_000343"
     CDS             23558..24634
                     /gene="carA"
                     /locus_tag="IXF54_000343"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1403628.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24639..27824
                     /gene="carB"
                     /locus_tag="IXF54_000344"
     CDS             24639..27824
                     /gene="carB"
                     /locus_tag="IXF54_000344"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1403629.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27878..28654
                     /locus_tag="IXF54_000345"
     CDS             27878..28654
                     /locus_tag="IXF54_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1403630.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28651..29583
                     /locus_tag="IXF54_000346"
     CDS             28651..29583
                     /locus_tag="IXF54_000346"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1403631.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            29580..30296
                     /gene="pyrF"
                     /locus_tag="IXF54_000347"
     CDS             29580..30296
                     /gene="pyrF"
                     /locus_tag="IXF54_000347"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1403632.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            30303..30932
                     /locus_tag="IXF54_000348"
     CDS             30303..30932
                     /locus_tag="IXF54_000348"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1403633.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30920..31234
                     /locus_tag="IXF54_000349"
     CDS             30920..31234
                     /locus_tag="IXF54_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403634.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            31272..31913
                     /locus_tag="IXF54_000350"
     CDS             31272..31913
                     /locus_tag="IXF54_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1403635.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31944..32648
                     /locus_tag="IXF54_000351"
     CDS             31944..32648
                     /locus_tag="IXF54_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1403636.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            32645..33547
                     /locus_tag="IXF54_000352"
     CDS             32645..33547
                     /locus_tag="IXF54_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1403637.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33705..35411)
                     /locus_tag="IXF54_000353"
     CDS             complement(33705..35411)
                     /locus_tag="IXF54_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1403638.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            35601..36224
                     /locus_tag="IXF54_000354"
     CDS             35601..36224
                     /locus_tag="IXF54_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403639.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36674..37723
                     /locus_tag="IXF54_000355"
     CDS             36674..37723
                     /locus_tag="IXF54_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1403640.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37720..38601
                     /locus_tag="IXF54_000356"
     CDS             37720..38601
                     /locus_tag="IXF54_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403641.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38604..39374
                     /locus_tag="IXF54_000357"
     CDS             38604..39374
                     /locus_tag="IXF54_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403642.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39653..40507
                     /gene="aroE"
                     /locus_tag="IXF54_000358"
     CDS             39653..40507
                     /gene="aroE"
                     /locus_tag="IXF54_000358"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1403643.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40685..41848
                     /locus_tag="IXF54_000359"
     CDS             40685..41848
                     /locus_tag="IXF54_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1403644.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41965..42375
                     /locus_tag="IXF54_000360"
     CDS             41965..42375
                     /locus_tag="IXF54_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1403645.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            42549..44777
                     /gene="topB"
                     /locus_tag="IXF54_000361"
     CDS             42549..44777
                     /gene="topB"
                     /locus_tag="IXF54_000361"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1403646.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44915..45232
                     /locus_tag="IXF54_000362"
     CDS             44915..45232
                     /locus_tag="IXF54_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403647.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            45392..45628
                     /locus_tag="IXF54_000363"
     CDS             45392..45628
                     /locus_tag="IXF54_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1403648.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45625..46023
                     /locus_tag="IXF54_000364"
     CDS             45625..46023
                     /locus_tag="IXF54_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1403649.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(46690..47634)
                     /locus_tag="IXF54_000365"
     CDS             complement(46690..47634)
                     /locus_tag="IXF54_000365"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1403650.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(47930..48874)
                     /locus_tag="IXF54_000366"
     CDS             complement(47930..48874)
                     /locus_tag="IXF54_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1403651.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            49095..50309
                     /locus_tag="IXF54_000367"
     CDS             49095..50309
                     /locus_tag="IXF54_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1403652.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            50331..52346
                     /locus_tag="IXF54_000368"
     CDS             50331..52346
                     /locus_tag="IXF54_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1403653.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(52429..53802)
                     /locus_tag="IXF54_000369"
     CDS             complement(52429..53802)
                     /locus_tag="IXF54_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1403654.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(53828..54112)
                     /locus_tag="IXF54_000370"
     CDS             complement(53828..54112)
                     /locus_tag="IXF54_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1403655.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(54114..56129)
                     /locus_tag="IXF54_000371"
     CDS             complement(54114..56129)
                     /locus_tag="IXF54_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1403656.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(56308..56613)
                     /locus_tag="IXF54_000372"
     CDS             complement(56308..56613)
                     /locus_tag="IXF54_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1403657.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(56702..57733)
                     /locus_tag="IXF54_000373"
     CDS             complement(56702..57733)
                     /locus_tag="IXF54_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1403658.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            57940..58815
                     /locus_tag="IXF54_000374"
     CDS             57940..58815
                     /locus_tag="IXF54_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1403659.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(58967..59809)
                     /locus_tag="IXF54_000375"
     CDS             complement(58967..59809)
                     /locus_tag="IXF54_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1403660.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(59901..61778)
                     /locus_tag="IXF54_000376"
     CDS             complement(59901..61778)
                     /locus_tag="IXF54_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1403661.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(61775..63196)
                     /locus_tag="IXF54_000377"
     CDS             complement(61775..63196)
                     /locus_tag="IXF54_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1403662.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(63199..66588)
                     /locus_tag="IXF54_000378"
     CDS             complement(63199..66588)
                     /locus_tag="IXF54_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1403663.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            66845..>67213
                     /locus_tag="IXF54_000379"
     CDS             66845..>67213
                     /locus_tag="IXF54_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1403664.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYL"
BASE COUNT        21193 a        11962 c        13866 g        20192 t
ORIGIN      
        1 cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga
       61 aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg
      121 tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca
      181 aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga
      241 aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg
      301 aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga
      361 tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca
      421 gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt ctggaacact
      481 gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa
      541 aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac
      601 cgtttattcg gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc
      661 aacaagtaaa acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa
      721 gatggaaatt ttatgtattt ttattggtct atgcaataaa taaattaaat attcagaata
      781 caattgattt taacgaaagg gcttctatca atggttgtgt tgtatttatc tgcccaggta
      841 gagtaaaata gatcattatc taaaaggtaa ttgttcatac tatataagtc atttgaaagt
      901 aaaaagatac tgtaaataaa aaatgtggtt aaataattct cataatcagt gtaagcgtta
      961 tcaaaaatat agcatacttg ctttgtagat gaatgacacg atatatgttt ggtatgttct
     1021 gcaagacaga tgaatatgga ggtggagaca atgtcttcca aagaactaac aatgtattta
     1081 gagaaagaat tggatcaact gaaagaaaag gggttataca atacgatcga catactagaa
     1141 agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct agcatccaac
     1201 aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga agcaaccgaa
     1261 acttatggtg taggagcagg agcagtccga acgatcaatg gttcgctgaa aatccatcag
     1321 caattagaag aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc ctttcaatct
     1381 ggattcaact gcaatatggg cgctatctct gcagtaatga caaaagaaga tgctatctta
     1441 tcggatgaat taaaccatgc atctattatt gacggttgcc gcttatccgg agcaaaaatc
     1501 atccgaatta aacatcaaga tatgaaagac cttgagaaaa aagcaaagga agcgatagaa
     1561 agtaagaaat ataaaaaaat catgtatatc acagatggtg ttttctccat ggatggagac
     1621 attgcacgat tgccggaaat tattccgatt gttgaaaaat atggactgat cacctacgtc
     1681 gatgatgcgc acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa gcattttgga
     1741 ttaagcgaca aaatcgatat gcaaatgggc acgttatcga aagctatagg tgttgttggg
     1801 ggttatgtag ctggatcgaa aacactaatc gactggttga aagctagaag tcggccattc
     1861 ctattttcta cttcgctaac tccaggtgca gctgcagctg cattggcttc gattactttg
     1921 atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa atgctaatta ttttaaagaa
     1981 gaattgaaaa aagtaggcta taacatcggt atgtctgaaa cacctattac tccagtcatt
     2041 ttgggagacg aaaaagtgac tcagactttc tctaagaagc tgatcgaaca tgggatttat
     2101 gctaaaccaa tcgtttatcc aactgtgcct cttgggacag gcaggatcag aaatatgccg
     2161 actgcggaac atacgaggga aatgcttgat gaagcagtag cagtttatca aaaaatagga
     2221 aaagaaatgg aaattattta actaaattta gttttttcgt ctatttaaaa ttagagaaca
     2281 tgttcacgca tatattgtta atcatctgaa gatagtagta aaaaagatca gggaggatca
     2341 tcatgaaaaa agtattggtg acaggatgtt taggacaaat tggttcagag ttgatcacac
     2401 ggctacgtca agaactaggc agtgaaaatg tcattgcgac agatatacgc atgcctgaag
     2461 tcattgagaa aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca
     2521 gcatagcaga acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg
     2581 ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat
     2641 tggaagtcgc tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg
     2701 gtccaaatac tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt
     2761 atggggtaac gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg
     2821 tagatactcg tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg
     2881 gtacgacaga ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta
     2941 gtttcattga tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca
     3001 ttataaaact catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat
     3061 cggcgatgtc ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt
     3121 ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt
     3181 cactagatgt ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg
     3241 tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca
     3301 agaaccacat ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca
     3361 agggatggaa gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa
     3421 acgtacgatg ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg
     3481 ggtatttttt aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt
     3541 ttcttggttt ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc
     3601 agctgctatt ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac
     3661 agcagctttt ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc
     3721 cttgtcgatc attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat
     3781 tgttcccgta gctgttacag ctgctgtatc gatcctgttg atcaattttt taggttctaa
     3841 agctggaggg gcttttcagt cgattacgct tgtctgcaag cttattcctt tgtttgtgat
     3901 cgtcattttc ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc
     3961 aggagaaaat ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc
     4021 atacgacggt tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga
     4081 tttgccaaaa gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa
     4141 tgcggtgttc ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag
     4201 tgacgttgca aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt
     4261 gatttctgtt tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt
     4321 tatggcaaaa gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac
     4381 taaagttcct gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat
     4441 cggtggtttt gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat
     4501 ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa
     4561 agttccaatg tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc
     4621 aagcacgctt atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg
     4681 tatcccgatt tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac
     4741 tgcacttctc tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca
     4801 aaagtactcg ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt
     4861 aaaagacaga aatcgggctc aaccgatttc tgtcttttac tttgatttgc gtaactgaac
     4921 tattcggcgt acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg
     4981 aagccaaatc agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt
     5041 atccatttca agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga
     5101 aataatcagg agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat
     5161 cgtagcataa aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat
     5221 ctcgacaccc cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct
     5281 cgttacttgc ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga
     5341 ggagtcatat ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt
     5401 tagccttagc gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc
     5461 tagtaacaag ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt
     5521 tggtgatctt accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg
     5581 agctgcattt atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg
     5641 aacctagtac ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg
     5701 ttttattact cataccattt ggcatttatt taagatatta tttccggctt tcttttatga
     5761 aaacgatgat tgctagtttc tgcctttctc tattttttga attgacgcag ctaagtggac
     5821 tttattttat ctacccaaga ccataccgct tatttgatgt gaatgattta gtgacaaata
     5881 cattgggtgg aatcattggt tttgtcgtta cgccgttatt tacatttatg cttcctacaa
     5941 gaagacgaat ggatgaactt tcttatgaaa aaggaaagac tgtctcattg actcgacgct
     6001 tggtcgctta tttgatcgac tggacatttt tatctcttat taatttggta gcggcgattt
     6061 tctttaaaat tgcgttcgat tcaggggcag ttacttctca tatctggtgg cttttattag
     6121 aagtaggggt atactttatg attgtaccgt ttataaccaa tggatggaca cttggcaaaa
     6181 aaattgtgcg tatccgtttg gtggaagaag accgcgaacg aatttcttta aaagcattga
     6241 tgatccgcta cgggtatctg tactttcttt tttatggttt aagctggttg gggcagttgc
     6301 ttcagcaaat catgaattcg acaacgaata cggtgttgca gatgagtgca gtattcattt
     6361 ctctcggtgt cttgttacta caaggtattt ttattttaaa tatcttgcta tcatttttga
     6421 gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac atatactgtt agcacgattg
     6481 gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa aaacgctttg tctgtgttat
     6541 aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg aatgaataat tttgatatat
     6601 tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa cagcccacag
     6661 aggtccagga acgtttgatt cctttaattg caaaaggaaa aaatatcatt gggcaatctc
     6721 aaactggtac aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa atcaatgcag
     6781 aaaaagagga agtacaagtt gttattacag ctccaagtag ggaactagct gcacagatct
     6841 atgaagcagc tacacaaatc gctaaatttt ctacaccgga gatccgtgtc tcgaattttg
     6901 tcggcggtac agacaaacag cgtcaattaa atcgtctgca tcaccaacag ccacaggttg
     6961 tagtaggcac gcctggtaga atattagaca tgatgaatga acaagcgctg aagattcaca
     7021 ccgcatttgc atttgttgtc gatgaagcgg atatgacatt ggatatgggt tttttaaatg
     7081 aagtagacca aattgcaagt cgattaccag aaaagctgca gtttttagtt ttttctgcca
     7141 ctattccaga aaaacttcgt ccattcttac gaaaatacat ggaaaatcca atcattgaag
     7201 aaatcaaacc aaaagcagtt atttctgaag acattgaaaa ttggctgatt tcaacaaaag
     7261 gtaaagatga gaatcaaatg atttatcaac tactgacaat tggtcatcct tatttagcaa
     7321 tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc cgattacttg aaaaaccaag
     7381 gcttgaaagt agcaaagatt catggagaca tcaccccaag agagagaaaa cgagtgatgc
     7441 gacaagtaca gaatctggag tatcaatatg ttgtagcgac tgatttagct gctagaggga
     7501 tcgatatcga aggtgtctct catgttatta atgcagaaat tccagaagat ctagacttct
     7561 ttatccatcg tgtcggacgt actggacgta atggcttaaa aggaacggcc attacgttat
     7621 attcgccaaa tgatgagcaa gccattgatg cgatagaaaa attgggcgtc tcatttgaac
     7681 caaaagaaat caaaaacggg gaaatcgtca aaacatacga tcgtaataga cgtgccaaac
     7741 gagaaaaatc aaaagatgta ttagatccca cgctgattgg tcttgtgaag aagaaaaaga
     7801 aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc aattgcagaa aaaaataaaa
     7861 aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat aagaaaaatt
     7921 cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct tgtttttaag
     7981 ctagactttt tttcattccg atacaaagta cttcatcttc aataaaaggt tctgtgcaag
     8041 tatgataccc ttgtttttca taaaaatcta cagccgtcat ttcagctact acataactag
     8101 tctgacaacc ttctgaaacc gcccgttttt cagcagcttc aagcaactga ccgcctaacc
     8161 cttttcctcg gtattctttt gctacgcaaa atcgttccgg cttgaagcaa gtaggtgttt
     8221 tcttttcata acgcaaagta gctactggtc ttttttgatc aagaaataga taataggttt
     8281 gttgcgggat atcttttccg tcgaattcta gttctgcagg aatcccttgt tctaatacaa
     8341 aaacttgata tctcaaaaag cagctagctg cgcgtatcca ttcatccgct ccaactattt
     8401 ttttcatcat attcctcctt gtcatgaata gcagagatgt attgattcct ttattcttat
     8461 cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg agatattgac
     8521 agtgatcaat agaatgcgta tactaaataa gtacgaaaag gatatgattt gttttttaag
     8581 actcacagaa aattctttct tggctgaaaa aagaatagtc actaatcaaa ttgctccctt
     8641 tttctatttt ggtagaaata ccgacgcagc tcagcgttat cgagtttaag aggtaatgat
     8701 tacacatcat tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt gatggtgttt
     8761 ttttgtattt aatttataga aagcaggtaa tgacatgaaa caattaagta gtgcacaagt
     8821 tcgacaaatg tttcttgatt tcttccaatc taaaggacat acgattgaac ctagtgcgtc
     8881 tttgatcccc gtgaacgatc caaccttatt gtggatcaac tctggtgttg caacattgaa
     8941 aaaatatttt gatggttcag ttgtgccaga aaatccaaga atcacaaatg cccaaaaatc
     9001 tattcgaaca aatgatatcg aaaatgtcgg taaaacagca cggcaccata cgatgtttga
     9061 gatgctgggt aatttttcga ttggtgacta tttcaaaaat gaagccatcc actgggcatg
     9121 ggaatttttg actgatccaa aatggatggc attcgatcca gaaaaacttt atgtaacagt
     9181 ttatccaaaa gatacagaag caaaacgcat ctggcatgaa gaagttggcc tgccactaga
     9241 tcacattatt gatgtagaag acaacttctg ggatatcggt gcaggcccat gtggtccgga
     9301 tacggaaatt ttctatgacc gtggagaagc ttataacgat ttagcagaag acgatcctga
     9361 aaactatcca ggaggagaaa atgaacgtta tttagaaatc tggaacttag tattttcaga
     9421 attcaaccat aagcctgata acacatttga accacttcct cataaaaaca ttgatacggg
     9481 gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact ttgagacaga
     9541 cttgtttatg cctattatcc acgccgtaga aaagctaagc aaacaagtaa aatatggtga
     9601 aaacccagta acagatattt cttacaaagt aatcgcagat catattcgcg ccctttcatt
     9661 tgctattggc gacggtgcat tgccatcgaa tgaagggaga gggtatgtct tacgccgttt
     9721 gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt gaacaagcat tcttatatcg
     9781 tttagtccct gttgttgggg aaatcatggt aagttattat cctgaagtac ttcaacaaaa
     9841 agaatttatt gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa caatcaatga
     9901 aggtttggaa atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg ggacgactct
     9961 agcaggaaaa gacatcttca aactatacga cacatatggc ttcccggtag aattaacgga
    10021 agaggtggca gaagatgaag gactgaaagt tgatcatgct ggttttgaaa aagaaatgga
    10081 tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg gggtacaatc
    10141 tgctttattg acagatatca aagtagaaag taaattcatt ggatacacag aacaagaatc
    10201 agatagtcaa ttattggtca tcgttaaagg tgagtctctt ttgaatgaag tcgcagaagg
    10261 tgaagctcaa atgatctttg atcaaacacc tttctatgca gaaatgggtg ggcaagtagc
    10321 ggatcaagga tggatcatga atgaagtagg cgaaatcgta gcagttgtta cagatgttca
    10381 aaaagcgcct aatggtcaat ttatgcacac cgtagaagta aaagcaccaa tgagtgaagg
    10441 acaaacctat catctattga tcgatcaatc gatgcgcaat cggatcatca aaaaccatac
    10501 agcaacccat ctactgcaca aggcattgaa agaagtactt ggcgagcatg caaatcaagc
    10561 tggttcttta gtcgctcctg gacatttacg attcgacttt acccactttg gtcaaatcac
    10621 ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg aagctatccc
    10681 agttgaaaca attgaaacag atatcgatac agccaaaaac atgggtgcaa tggctttgtt
    10741 tggtgaaaaa tacggacatg acgttcgagt agtaaatatt ggcggctggt caatcgaact
    10801 gtgcggaggc acacatgtag caaatacaga aaacatcgga atcttcaaaa tcgtttctga
    10861 atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag aagcgtacga
    10921 actacttgaa gaagaagaaa aacaactaaa acaagtagca acaattgtta aatcacctca
    10981 attgaaagaa gttgtttcaa aagcggaaca attgcaacag caagtaagag agctacaaaa
    11041 ggaaaatgaa caattagcta gcaagttagc gaatcaacaa gctggtgatg tattcaaaga
    11101 tccagaaacg atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga aagatatgaa
    11161 tcagctgcgt caattagctg accaatggaa acaaaaaaat atttcagata ttcttgtttt
    11221 agcaaacgaa caagaaggaa aagtcagtct gctagcggca atgacgaaag aatcaaatga
    11281 agctggacta aaagctggcg acttgattaa agcaatcgca ccgaaagtag gcggtggcgg
    11341 tggtggacgt ccagacatgg cacaagctgg cgggaaaaat cctgctggta ttccagatgc
    11401 acttaaagct gcgaaagaat gggttgcgca acaatagggc gtttacgttt gacagaaaaa
    11461 caaaagttca aacattgaac gaaacatgat tatgggggcg aagacaaaca agtcttgccc
    11521 tcatactttt ttcaattgga tggcgcatct aatcaaatgg tcggattcgg atggtcttta
    11581 gttgacgaaa gtgacagaaa tttttacgat tagtataaat aaaaaggggt gaaaaaatga
    11641 atagtagaaa taagaccttt cgactagtct tacgtgcgat cttgttagca atcatcatcg
    11701 tacaagcgat ggttccttgg ctggggttta ttccattagg attcattagt ttaacaatta
    11761 tccatatcac agtaattatt gctgcagtag tattaggccc aaaagatggc atggtgattg
    11821 gcttgttttg gggaatcgct acaattgttc gtgcctatgc catgccaaca acgccatttg
    11881 atacattagt gttcacgaat ccgattatct cggtagttcc cagagtactt gttggcttag
    11941 tagcaggctt ggttttccac tggatttatc aaaaaaataa atcaataacg atcagctcag
    12001 cctttgcagg ggtactgggt tcattggtca atacagtctt agtgttagga ttcatgggat
    12061 tattttatac aggtgcaacg gcagatgcct atggtgtaga ctctgctctt ttatttaaaa
    12121 ccttagcagg ggttgcagca atcaatggga taccagaagc aataggtgcg ggaatcataa
    12181 caccattact agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa taatgaccat
    12241 cttttaatag taataggacg cacgatagat agaaaaagga agagaggaga ggaaaacaat
    12301 cgtcctctcc atctttttac ctgagcaaca agcaaactag aaggggtaaa aaacttggct
    12361 aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa aggagtacta
    12421 gcgggtatct taggattggt tacgaatctg atcttgtttg tctctaagct aatgatcggg
    12481 cttttttcag gaagtgtctc cattatggct gatgcagtga acagtctatc agatactgct
    12541 tcatccatat tgactttgat cggatttaaa attgctgcaa agccagcaga tcaggaacat
    12601 ccttatggac acgaacgctt cgaatatatc agtgggttat ttgtttcgat catcattact
    12661 tatgtaggat ttcaattttt agagtcttct gctgaaaaaa tcatccatcc agaaaatgtc
    12721 tctttaacac ccatcgtctt ttttgtccta atcatttcca tctttttgaa atttgcacaa
    12781 ggcaaaatgt atcaggttat tgcgcaatcc attgattctg aaacattgag agctacagca
    12841 acagatagtt ataatgatgt ctttactaca ctggctgtat tgctttcagc agcaatcgaa
    12901 tgggtaactg gatggcgtat tgatggatat ataggttttg ctttagcatt atatatcctt
    12961 tatagtggat tgacgatgct tcgaaatttt gtatacgaac tattaggaag tcgcccaacg
    13021 ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat acttggctat
    13081 catgatttat tggtgcataa ctatggaccc aataaacgct ttgcatcagt ccacatcgaa
    13141 gtagatgaat caatgaattt gaatgtggct cataagatta ttgatctgat agaaaaagac
    13201 tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc tgttgataac
    13261 gaaaaatact ataatatctt gcggcagtta aagcaaattc ttcttgaaac aggtacaggg
    13321 cttaatattc atgattttag agtaattcat caaggaaaaa cgctgcaatt tgatgtagtg
    13381 gtccctgaga aattccagtt tgttgataca gaactagaaa gaatcttacg tagtcaaata
    13441 gaagaaaaaa tcggagatta ccaattggaa attacgtttg atcacaatta tcttttatat
    13501 taaatgagaa actataaaaa gactccttaa agtttttaat ttaaagagtc tttttttctt
    13561 ttaattgttt atggagagcc tgacgtgcaa gattatctgc tcctttattt ttgttttcgg
    13621 gcaaccattg caggatcaat aaagaaaatt cggatagaag ttcttggatt ctttgaaggt
    13681 agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact agtgttttgc
    13741 tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt tttctttaga aattctaaag
    13801 tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc tatagaaagc gggatagata
    13861 gctgttcttg tttaccttga tagacaatca agattccacc tccactagga ccagggttgc
    13921 cttttgtaga ggcatcaatg tatactttta acataaaaag agattctcct ttttgttcaa
    13981 actaatgtta tggtaagatt atatcattac tgaatcggtg agaggttaga gaatgaaaaa
    14041 aaatattcga tggcagcctg aactggcaca tacgatcatc tattggtcat tgaccttgat
    14101 cattctgttt tttagtctga ttctttcttt agaaaacaca ggtccttatt ggaaaagcaa
    14161 ttttgtttta ggtatttttc ttgtttttct atttctaggt ttgcggcgtt cttttatttt
    14221 aaaatcaaac tctgtgatta tacgttatgc tgctttttgg aaagaccgag aaatatctgt
    14281 cggacagatt cgatctttga cgccaactaa tactggggtg caaatgatgt tgcaagatga
    14341 gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt ttgatcattt
    14401 tgaaaaaaac ggacccaaat cagaatcaat tccttatcct tatacgaaaa aaagaaaaag
    14461 ctaattttaa aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa aagagtgaga
    14521 cgtacggttc atcccgttta gcgtctcact ctatttgttt atctattcat gcatactagt
    14581 atcggtagtc tgaacgttgc ctttcttgac atgggcagct tgtgttcctc gattcccttc
    14641 gatgatctca aattcgacca cttgattttc ttccaaagtt ttgaatccct cttcttcaat
    14701 cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc cgtatccttt
    14761 tttgttgtca aaccatttta caattcctgt ggtcaaagca ctcacgacct ttctaaagtc
    14821 tgatcatagc cttcattata aagagtattt ataagtacgt caaataaatt aaaaacctgt
    14881 aattttatgt tttttttctg aataaaaaaa ggtataattg gcatatctta acaaagaaga
    14941 tattttgttg aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat ctgcaaattt
    15001 cacaagaaac agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag
    15061 acgatttgga actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat
    15121 caaaggataa cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg
    15181 gcgaaggaaa gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa
    15241 aatctgttat tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg
    15301 ctgcaggcgg aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg
    15361 gtgacatgca tgctatcaca acagctaata atgcattatc tgctctttta gataatcata
    15421 ttcatcaagg aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg
    15481 atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg
    15541 ttccaagaga agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc
    15601 ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat
    15661 ttgaccgtca gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc
    15721 tgaaagatgc aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc
    15781 acgggggacc gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag
    15841 cattgagatt agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag
    15901 agaaattttt agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg
    15961 tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg
    16021 agaatcttga tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg
    16081 aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg
    16141 aactcacact gttggagcac ttgtgtgaaa atcaggggat tcttgccaag agagcgagtg
    16201 tatgggcaga tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg
    16261 atagaaaaga agctaattac aagccattat accgcctaga agaaacgata caagaaaaga
    16321 cagaaacaat cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc
    16381 aaaaacaaat agaagagttt acgaagaatg gatgggacaa actgccgatt tgtatggcaa
    16441 aaacacagta ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca
    16501 tcacgatccg cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag
    16561 atgttatgac gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa
    16621 atgacggaga agtgttggga ttgttttaag cttgattata atctaccatc gaaggagctg
    16681 tattttgact acagcttctt tttttagacg atagggaatt aattttgttt acactaggat
    16741 cattcttttt ctttattgta ttttgagcta tactaaagca taataggagg gtatgatgaa
    16801 aacgacattt ataagaaaaa ccggttttta cggtatgggt agctcgattc agctaagaat
    16861 caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg atctggaagt
    16921 gccctttacg atgcaggtca ccttttattg gctaaaaagc cccgtttata cagtgaaaga
    16981 agcaaagcct gcttatgtga tcacaatgaa ttacttgatt cttcaaatct atccgttttt
    17041 atttctagca actgggttgg gggccgtttt cctacaaaat ttatttttca gtttttttgc
    17101 agttattgtg ttgattgcgt tctttttcta tataaaaaac cgggcatatt tgatcaagga
    17161 ggaaaattat ggcaaactct gatctttttt taatcagttt caatcgaatc gaaaaatgga
    17221 tgagagacga gatgggaaat gcacgaaata tgggatttac cgaacttgtc agacgcctag
    17281 ctcaaagaaa acaattaatg atcagaaaat acgaagatga tcttttacag cttgctcagt
    17341 taaggaatgc gatcgtccat gatcggatcg cagtcgattt tgtcattgca gagccaaatg
    17401 attggacagt caagcgaatc cagctgatcg aacaagaact gattcagcct gaaaccgtgc
    17461 tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta cttgaactgt
    17521 taaagatagt agcagaaaaa aggtattcac aatttccgtt atacaataaa ggaaaatttg
    17581 tagcattgat tactttacgt aatatcgggt tttggctagc aaaagaaagc caaaaaggtc
    17641 cggttgattt aacaaataaa cgagcgttgg atttgatcat ccaaaacgga aagtacacaa
    17701 attatcattt tgttccagct aaaacacata tttacgaggt tgaagcgatg tttcgtgagc
    17761 aagggacttt ggaagccgta cttatcacta aagatgggaa tcccgatggt aatctgctcg
    17821 ggatcgtacg acctcgtgat atttataaac aaatagaaaa ggattgattt tttgttaatt
    17881 atttattgga tattcagcgc tttgctagta ggagtggatc agttggtcaa atggtggatc
    17941 actgataatc tagcgcttgg agagacgaaa aatcttatac caggtatttt gtcactgaat
    18001 catattcgaa acactggagc cgcatggagt atgctagaag ggaagatgtg gttctttact
    18061 atagtgacac tcattgcagt cgttgtgatt ctcacattga tgattaaaaa tcgtgaaaaa
    18121 ggtaatcgct ggttcatgat tggattgagt ttgattctag ctggtgcgat tggcaatttt
    18181 atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc agacagattt tatgagcttt
    18241 ccgattttta acgtagcaga tgtgacactt gtatgtggag tgatttgtat gttaatttat
    18301 attattttag atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg acgattcacc
    18361 atgaatctgg taggatcgat aaagtattga ctaatttatt caccgattac agtcgttcgc
    18421 aaattcagct gtggctgaaa aatggagcgg tttctgtcaa tggacagcca gtcaaagcaa
    18481 attacaaagt aaaacaagga gacgagatta ctgtcttgat ccccgaacca gaaacattag
    18541 atgtggtccc agaaaatatc ccgttgacga tcgtttacga agatgaagat gtagcggtga
    18601 taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat ggcacaatgg
    18661 tcaatgcgct gatgtaccat atcaaggatc tttctacgat caacggcgtt attcgtccag
    18721 gtattgttca ccgaatcgac aaagatactt ccggactgct aatggttgcc aaaaatgacc
    18781 aggcacacga atcattagct cagcaattaa aagataagac ctctttgcgc aaatatgttg
    18841 ctttagtcca tggagagatt cctcacgaaa aaggaaccat caatgcgcca atcggccgtt
    18901 cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg gaagtcggct gtcacacatt
    18961 ttactgtatt ggaacgtttc gaaggattta ctctggttga attaaaacta gaaaccggaa
    19021 gaacacacca gatccgtgtg catatgaagt atatcggtta tccgttagct ggagatccag
    19081 tatacggacc gaagaaaact ttgaaaggca atggtcagtt tctccatgcg caaatgttag
    19141 gttttacgca cccgactaca aacgaaaaga tgatctttga agcacctctt ccagaagttt
    19201 ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga taacggtata
    19261 gtaattaaca acgaaagcat aaaacttaat agaattcctt taagtttagt cccgtgaggc
    19321 taagaagggt gggagcaagg atacgtatgg tgcgccataa gtagattgct cattgacata
    19381 gtatagctat cagtgtatca gtatacgctc agtctaaaca ttcctcctgt cctcttaggg
    19441 caggagtttt tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa gaagtagtag
    19501 atcaggtaac catgaagcga gcattgactc gaattactta tgagattatc gagagaaacc
    19561 atagtatcca agatatcgta ttggtaggta tcaaaacacg aggaatctac attgcatcaa
    19621 gaattgccga acgcctgaag cagctagaag atatcgatat tcctgtagga gaactggata
    19681 tcactttata ccgagatgac aaaaaagaaa atccagaaga accggaactt cattcttctg
    19741 acatcccagt ttccttagaa ggaaaagaag ttattttgat tgatgatgtt ttgtatacag
    19801 gacgaacgat tcgagcagca atggacgcag taatggattt tggcagacca agaaaaattt
    19861 cattagcagt cctggtcgat cgtgggcatc gagaattgcc aatccgtgca gattatgttg
    19921 ggaagaacat tccaacatct cgggcggaag agattttagt agagatgcaa gagcttgatg
    19981 gacaagatcg catcatgatt ttgaaagagg aagattagag gagagagaaa catgtcggag
    20041 aaaaaaggat ttcataatga tgacgtggtg ttagatatcc acgatcgccc aaccccagga
    20101 cattgggtag gattaagttt acaacacttg ttcacgatgt tcggagcaac cgtgttagtc
    20161 cccatcttag taggtatcga tccaggtatt gcgctagtca gttcaggact aggtacattg
    20221 gtgtacttat ttactactaa aggaaagata cctgcgtact taggaagcag cttcgctttc
    20281 attgcagcga tgcagctttt gatgaaaaca gatggttatc cagcgattgc acaaggagca
    20341 gtcacaactg gattagtcta tttgattgtt gcgctgatca tcaaaaagat cggttcagca
    20401 tggctggata aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt tatcgggttg
    20461 ggacttgcgg caaatgcagc aacaaatgct atgtataatc agggcacgta tgatttcaag
    20521 tatattgctg tagcgctgat cacattagga ttaacaattt tcttcaacat gtggctaaga
    20581 ggttttctcg gattgattcc gattttacta ggtattatta gcggatacat cgtagccttg
    20641 ctgtttggaa tcgtggatac taagccaatc gtagacgctg cctggtttgc tgttcctaac
    20701 tttgagattc cctttgtaca atatgaacca aagctatatc ttggagcaat cacaacaatg
    20761 gcacccatcg cttttgtgac gatgacagag catatcggcc atcttatggt attgaataaa
    20821 ctaactaaac gtaatttctt tgaagaaccg ggtcttcata agacgttgac aggtgatgga
    20881 ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta cggtgaaaac
    20941 attggtgttc tggctatcac acgtgtccat agcgtgtttg tgatcggtgg agctgcagta
    21001 tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag tatccctgga
    21061 ccagtcatct caggaatcag ttttatctta tttggagtaa ttgccgcaag tggaatgaag
    21121 attttaatcg agaacaaaat cgattttgat aaaaagaaaa atctgttgat tgcttcagtt
    21181 atcttagtag taggaattgg cggactcgtc tttgaagtag gcacatttac attgtcggca
    21241 atggcattag caactgtact tggcatcatt ttaaatctta tcttaccaga aacttcacga
    21301 agcgaagaaa actaaggagg aaacattatg atcatcaaat cagaacgtat cagcttaaag
    21361 catttattga ccgttgaggc acttactgat caagaagtta tgggcttgat ccgcagaggg
    21421 caggaattca agcaaggtgc taattggact ccgcaaaagc gccagtactt tgctacaaac
    21481 ctctttttcg aaaatagtac tcgcacacat aaaagcttcg atgtagcgga gaaaaaacta
    21541 gggctagaag tgattgaatt tgaagcaagc acaagctctg tccaaaaagg ggaaacacta
    21601 tacgatacag tactgacgat gtcagcatta ggcgtagacg tagcggttat ccgtcatggt
    21661 gatgagaact attatgacga actgatacaa agtaaaacaa tccagtgttc gattatcaat
    21721 ggaggagacg gaagcggaca gcatccaacc caatgcttgc ttgatttgat gaccatttat
    21781 gaagaatttg gtcattttga aggtctgaaa gttgccatcg taggtgatat cactcactct
    21841 cgcgtagcta aatccaatat gcagatgtta aaacgtctag gcgcacaagt attcttctca
    21901 ggtcctagag aatggtacga cgatgaattc gaagtgtacg gacattatat gccattggat
    21961 gaactactag atcaagttga tgtcatgatg atgcttcggg tgcaacacga acgccatgat
    22021 ggaaaagaaa gtttctcaaa agaaggctac catcaggaac atgggttaac agttgagcgt
    22081 gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag caccagttaa ccgtgatgta
    22141 gaattagctg attcattagt ggaaggatta cagtcgcgga tcgttcagca aatgagcaat
    22201 ggagtattcg tcagaatggc aatcttagaa gcagtgctaa atggaaaagc gtaattatag
    22261 gaggatgaaa aaatgaaaac actcattaaa aacggtcaga tcaatacacg aaaaaatatg
    22321 acgacaccag cagaaatttg gatagaagat gggcgaatca aagcaatcgg tacaggattc
    22381 tcagaagccg aatttgatga agtgtttgat gcaaagggac aattgatcac acctggttta
    22441 gtggacgtcc atgttcattt gagagagcca ggatttacat acaaggagac gatcgaagct
    22501 gggactcgct cagctgctcg tggcggtttc actactgtat gcgcgatgcc gaatttaaat
    22561 cctgtacctg atacggcaga aaagctgaga caagtatatg acatcattag aaaagatgca
    22621 gttgtgaagg tattgcagta tgcgccaatc acagaaaatc tgcgaagtga gaaattagtt
    22681 gatcaagaag cactgattga agaaggtgct tttgctttca caaatgacgg tgtaggtgtc
    22741 caaacagctg ggacgatgta tctggctatg aaagaagctg caaaaaacaa taaagcccta
    22801 gtagcccata cagaagatga atctttatta tttggcggcg ttatgcacgc tggcaagaag
    22861 gcagaagaac ttggtcttcc cgggatctta agcgtaaccg agtcttctca aattgctaga
    22921 gatctgttgc tagctgaagc aactggtgtt cattaccatg tttgccatgt ttctactaaa
    22981 gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac agcagaagtt
    23041 tcaccccatc atcttatttt gatcgacgaa gatattcccg aagatttcgg cttttggaaa
    23101 atgaacccgc cgctaagagg acgggaggac agagaagctt tgatcgaagg cttgcttgac
    23161 ggtacgatcg attgcatcgc tacagaccat gccccccatg gattagaaga aaaaagccaa
    23221 agtttcatga aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca actgatctat
    23281 actcattttg tagaaactgg tcgattcacg ttggaacaag tgatcaattg gttggcagtt
    23341 aaaccggctg agatttttgg tttgaatgca ggaacgctaa cggttggtgc ccccgctgat
    23401 gtagcagttt ttgatattac acaaacttgc acgatcgata aagaagactt tttatcaaaa
    23461 ggggagaata caccttttat cggctggaaa gtaaaaggcg aaacacaaat gacttttgtc
    23521 aacggaaaac ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc ttatattaga
    23581 agatggaaca gttttcgaag gaaaggcttt tggtgcagat gctagtgtct tcggagaaat
    23641 cgtttttaca acaagtatga ctggttatca agaaactatc actgatcaaa gttttaacgg
    23701 tcaaatcatt acatttacct atcctatggt gggtaattat ggggtaaata gagatgatta
    23761 tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa gaacatgcac gtgtagcttc
    23821 aaactggcga aaccaaatga cgctagattc atttttgaaa caaaaaggaa ttcctggaat
    23881 atcaggtatc gatacgcgcg cccttactcg caaaatccgt gaagtcggaa cgatgaaagc
    23941 tagtatagtt gatgctggag attccttcga acatgcattt gatcagctga aagcaaccgt
    24001 tctagcaacc aatcaagtgc agcaagtctc aactaacaaa ccttatccaa gtccaggaac
    24061 aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat agtattttac gagaattgag
    24121 taaaagaaac tgtaacctca cagtacttcc ctataacaca gatgccgaga cgattttagc
    24181 gctatcacca gacggagtca tgctgacgaa tggacctggt gatccaaaag atgtgccaga
    24241 agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg atttttggta tttgtctcgg
    24301 tcaccagtta tttgctttag ccaacggtgc agatacctat aaaatgaaat tcggacatcg
    24361 aggattaaat cacccggtac gagaagtagc gaccggaaga attgatttta cttctcaaaa
    24421 tcatggctat gcagtcgatg aagccacgat tgatccagaa aaactcatcg taactcatgt
    24481 agaagtaaat gacggaacag tagaaggagt caaacacaga gattatcccg ctttcagtgt
    24541 gcagtttcat ccagatgctg cccctggtcc gcatgatgca ctacatttat ttgatgaatt
    24601 tatggaaatg atggacgcag gaaaggagca gtaaacagat gccgaaacgt acagatatca
    24661 agaaaattat ggtgattgga tctggtccga tcatcattgg tcaagccgca gaatttgact
    24721 acgcaggaac acaagcctgc cttgccttaa aagaagaagg atatgaagtg gttttagtaa
    24781 attctaatcc tgcgacgatc atgacggata aagaagtggc agatcaagta tatatcgaac
    24841 cgatcacact tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat gctctccttc
    24901 caactttagg cggacagaca ggcctcaata tggcaatgga gctggcagca tcaggtattt
    24961 tagaagaatt gaacatagaa cttctaggaa caaagctttc tgcgatcgat caagcagaag
    25021 atcgtgatct attcaaacag ttgatgaaag aattgaatca gccaatcccc gagtctgaaa
    25081 tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa aatcggctat cctgtcatcg
    25141 ttcgtccagc ctttacttta ggcggaactg gtggaggtat gtgtgaaaat gaagaagaat
    25201 tacgtatcgt atcagaaaat ggattaagtc tttcccccgc tacccaatgt ttgattgaac
    25261 gaagtattgc aggttttaaa gaaattgaat atgaagtgat gagagattcg gctgataatg
    25321 caatcgttgt ctgcaatatg gaaaattttg acccggtggg tatccataca ggtgattcta
    25381 tcgtatttgc ccctagtcaa acattgtctg accatgaata tcaaatgctt cgtgatgctt
    25441 ctctttcgat tattcgagca cttaagatcg agggaggctg taatgtccaa ctcgctcttg
    25501 atccacatag ttttaattat tatgtgattg aagtcaatcc gcgtgtttct cgttcatctg
    25561 cgttagcaag taaggcgact ggctatccga tcgctaagct agctgctaaa atagcgcttg
    25621 gattaacatt agacgaaatg aaaaatcctg ttacaggaac gacttttgca gaatttgaac
    25681 ctgcattaga ttatgttgta gctaaaattc ctcgctggcc gtttgataag tttgaaaacg
    25741 gagaacggct tttaggcacg cagatgaaag caactggtga agtgatggct atcggtcgga
    25801 atatcgaaga gtctttattg aaagctgtgc gttctttaga aatcggtaca caccatatcg
    25861 aacttcctga gttggcagac atcacggacg atgacctaat ccaaaaagtg gttcgagcac
    25921 aagatgatcg cctgttttat gtagctgaag ctattagaag agggtacacc atagaagaat
    25981 tagcggattt gacaaaaatc gatttattct ttttagataa actgttgcat atcgtagaaa
    26041 tcgaacagca gttgaaacag cagccgttgg atgacaagat attgaaaaca gcgaaacaaa
    26101 acggttttac agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa gccgttcgcc
    26161 agatgagaaa agaaaatgat ttgattcctg tatataagat ggttgacact tgtgcagcgg
    26221 aattcgaatc aaaaacacct tatttttata gttcctatga atatgaaaat gaaagttcac
    26281 gatcagaaaa gccctcaatt ctagtactgg gatctggtcc tatccgtatc ggacaagggg
    26341 ttgaatttga ctatgcgact gtgcattctg tcaaagcaat ccagcaggca ggatacgaag
    26401 caatcatcat gaacagcaat cctgaaacag tttcgactga cttttctatt tcggataaat
    26461 tatattttga gccattgacc ttcgaagatg tgatgaatgt gatcgagcta gaacagccga
    26521 taggagtaat cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa cctttgactg
    26581 ctgctggtgt aaaaatctta gggacaacga tcgaagactt ggatcgtgcg gaaaatcgtg
    26641 atttattcga gcaagcgctg aaagagttgg atattcctca acctttaggg gatactgcta
    26701 caagtaaaga agaagccgtg gttatcgctt cgagaattgg ttatccagtt cttgttcgtc
    26761 caagctatgt attaggcgga cgtgcgatgg aaatcgttga aaaccagcga gatttagaag
    26821 attacatgga acatgctgtg aaagcatctc cagaacatcc agtactagtg gaccgttact
    26881 tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta ctcatcccag
    26941 ggatcatgga gcatattgag cgtgctggtg tccattcagg ggattcaatg gctgtgtatc
    27001 cacctcaaac attatctgaa tcgatcaaga aaacgatcga agattacacg atccgcttag
    27061 cattaggttt gaattgtgtc gggatgatga atatccagtt tgtcatccat gaaaatgagg
    27121 tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt tccattctta agtaaggtga
    27181 caggaattcc aatggcacaa attgcgacga aagctatttt gggagagaaa ctaaagacac
    27241 ttggttatga aaacggactt tatccagaaa caaaagaagt tcatgttaaa gcacctgtct
    27301 tttcttttac gaaattgcag aaagttgata cttacttagg gcctgaaatg aaatcgactg
    27361 gagaagtaat gggatctgac agacaattag acaaagcttt gtacaaggca ttcgaagctt
    27421 ctggattaca cttgcctgaa tatggagccg tattatttac gattgcggac gaaacaaaag
    27481 aagaagcact agaattagct aaacgtttta cagaaattgg ctatagtctg ataggaacga
    27541 aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta gcgaaaattt
    27601 ctgaggaagc agcagaaaaa aatgtagtcg acttaataag agatggtaaa acacaagtgg
    27661 ttgtcaatac gattgataaa gatcggggaa atgcttctaa agacggtttc atcatccgaa
    27721 gagaagcagt tgaacatggt attccattgt ttacttcttt agacacagca gatgcgatcg
    27781 ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat ttaacggcga gcacatgtta
    27841 agacaaaaag acgaaaaacc gttgcaggag gggacagatg aaacaagaaa tgatggaaat
    27901 cgttcagcaa aaacagctgg caccaaagat ttttgaaatg acattgaaag gtgatctagt
    27961 aaaagaaatg cagcaaccag gacagttctt gcatattctc gtgccgcgtg cagacttgct
    28021 attacgccga ccgattagct tgaatcatat cgaccacgaa acaaatactt gccgtatcat
    28081 ttatcgggtg gaaggtgagg ggactagagt cttctctact ctttctgccg gagaccattt
    28141 agatgtcatg ggtcctttag gcaatggttt tgatttgtcc aatcttcata caggagacga
    28201 agtatttata atcggtggag gaatcggtgt ccctcctcta tatgagttat caaaacagtt
    28261 agtcgaaaaa ggaattaagc caacgcattt tttaggtttt gccacacatg aagtcgttta
    28321 ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc gctactgacg atggaacata
    28381 cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg aagacggaac ctacagctgt
    28441 ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa cagctgtttt cttcgcatca
    28501 aaatgtccag ttatcattag aatcccgcat ggcatgtggg ataggggctt gctatgcttg
    28561 cgtttgtcat acgccggaag atgaaacagg aacaaaaagt gtgaaagttt gtgacgaagg
    28621 accgattttt aaaataggag aggtaattat atgaatgaga atcctttagc ggtcaaactt
    28681 cctggcctag aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg ttttggcaaa
    28741 gaatacggaa aatactatga tttgaatcag cttggatcga tcatggtcaa ggcaacgact
    28801 ccaaatgccc gctttggcaa tcccacgcca agagtggcgg aaacaccatc tggcatgttg
    28861 aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta tgcataaact tcttccagaa
    28921 ttagaggagt acaatgagtt acccatcatc gcaaatgttg ctggtgcttg tgaagaagac
    28981 tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat cgagctgaac
    29041 atttcttgcc caaatgtcaa acatggcgga attgcttttg gaacagattc tgatgttgct
    29101 tttcaattaa cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt caaattgtcc
    29161 ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg aagctggagg ggcagatggg
    29221 tttacgatga tcaatactct tcttggtatg cggattgacc ttaagacaag aaaaccgatt
    29281 ttagccaatc aaacaggtgg actctccgga ccagcaatca agcctgtcgc tattcgtttg
    29341 attcaccaag tagcaagtat atccagcctt ccaatcattg ggatgggcgg cgtgcagaca
    29401 gtggatgatg ttcttgagat gtttatggct ggagcaagtg ctgtagctat agggaccgcg
    29461 aatttcaccg atccgtatat ctgtcctaaa ctaattaaag agctccctgt gcgaatgagc
    29521 gaactaggca tcgaatcgct ggaaagatta atcaaagaag tgagagagga acgtgaacga
    29581 tgaatcaact agaccaacga ccaattattg cattagattt ttcaactagg caagaggtag
    29641 aagactttct tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc ggaatggaat
    29701 tgttctacca agaaggacct gaaatcgtac gttatctaaa agaggctggt cataatgtat
    29761 ttttagatct gaaacttcac gatattccga atacggtcga aaaagcaatg cggggattgg
    29821 caaagcttgg tgttgatctc acctgtgttc atgccgctgg cggtattcgt atgatggagg
    29881 cggcaatgcg aggattagaa gaggggactc cagaaggtgg aaaacgtccg ctacttctag
    29941 ctatcactca gcttacttct acaagtgaag aagaaatgca tgccgatcaa ctaatcgaag
    30001 tttctttaga aaaaagtgtg atccattatg ctagctgtgc caaaaaagca ggattagatg
    30061 gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga aatggcagga gacgaatttg
    30121 tttgcttaac tccgggtata agaccagaag gaacagtcgc aggagatcag acaagagtag
    30181 tcacaccttc tcaggcaaaa aagatcggct caacttttat cgttataggt cgaccaatca
    30241 ctcagtcaac taatccttat aaagcatatc aaacgattca aacagaatgg agctaaccta
    30301 aaatgaatgt tgaacaaagt atcgcaaaag acttattaga aattgaagca gtatttttaa
    30361 atccaagtaa tccttttaca tgggcaagtg gaatcaaaag cccaatttac tgtgataacc
    30421 gaatcacgat gagctatcca aaagtccgaa aagagatcgc aaaaggatta gcaagcaaaa
    30481 tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac tgcaacagca ggaatccctc
    30541 atgccgcatg ggtagcggaa atactggatt tgccaatggt ctatattcga agtaaggcga
    30601 aagatcatgg gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag aagatggttg
    30661 tgatcgagga tttgatttct acaggcggaa gtgtcttgga agcagctgaa gcagcaaaaa
    30721 gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac gtatgagttg ccaaaaggaa
    30781 aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat tctgtattga
    30841 ttgaagcagc tttagaagat cgttatatcg atgagcaaga attaactctg ctaaaagaat
    30901 ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt gatgaagata gagaaatccg
    30961 atttgtgtct ccagaatcat caattggaat agcaaaaatc caaaaactga ttgcgagaag
    31021 catcttttca tcaggtatga aaagaaactt ctatcgtgat cagtttttga tttttttaaa
    31081 attcattttt ctaagactgg ttagcagaaa gaagagaata atctcttctc ttttttctat
    31141 tatatataga ttttctctat tttttggtaa gggagctgtt tcaaaaatgg taaagagcgg
    31201 aaatgttgcg aatatcagtt atttaatgat atgatattcg gaaaaagaaa tctcaaaggg
    31261 aggtaacttt tatggggttg aatgaattta ttttaggatt gaacaattta gagacgatca
    31321 caagggcacc tggatttttt aaatatacag agcatacagt tgcagcacat tcgtatcgtg
    31381 tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa ccgatcgatt
    31441 ggaaactgct ctatgaaaaa tcattgaatc atgactacac agagcgtttt attggtgata
    31501 tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa acagttgaag
    31561 aaaagatgac ggacgaattc atcaaccaag agattccagc tgagttccaa gaagtttatc
    31621 gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg agaactatta gccatttcag
    31681 ataaggttga ccttctttat gaatcttttg atgaaatcat caaaaataac ccggaaaaag
    31741 tttataagga gatgttcatt gaggcagtga ctacgattaa aagctttgaa catcggcctt
    31801 ctgttcagta tttctttaaa gagatatttc cggaactatt gaaccaggaa ttttatggaa
    31861 aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa taaaaatgat
    31921 atgagatgca gggggaagta tagttgaaaa agaatatgga tgtcgaatgg ggctattctg
    31981 gagacaaagg tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga ggtgcggaat
    32041 ttccattgca gtcgcctatt catttgacca agggggaaga aaccaaaata gaaggagaca
    32101 cgctttcctt tcattatcaa aagcaacggt ttactgacaa ggaattcaaa aacacgattc
    32161 atttagtacc ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc tattatttga
    32221 cagatattca tttccacttg ccttctgaac atatcatcaa cggaaaacag gaagatattg
    32281 aatttcattt tgttcatatg aacagcaaaa aagagaatct agttgtgggt gtcatgtacc
    32341 agctaatggt acaggaaggt tggctgtata atcaagaaaa tggggaaagt tggaacttag
    32401 aaaaacatga gcattgggta aatccggatg tgttctttcc ggagcaaaaa agccatttcc
    32461 attatattgg ctcattgaca actccgccta ccagcgggcc aatccaatgg tttgtaatgg
    32521 atcatgtagg aaaactgaac caagagtttc ttctcccctt cgatggtcag tatgctcgac
    32581 cgaacaaccg accaatccag cccttgaatg gaagagaaat ttattatttc gaggagtttg
    32641 agcaatgaat attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg gtagtttcag
    32701 agaagcggca aagaaaatgt ttgtctccca gccaagtctt tcagctggta tcaaagaatt
    32761 agaaaccgag ttagggatca cgctgtttac tcgaacgaat cgaggggcat atttaacaga
    32821 agaagggcaa gacttcttaa atcgtgccga gaaaatattg gtccagcttg atacattaga
    32881 aactcactat ctaacgagtg aaaagagaga caatttttcg atcgctgccc agcattatga
    32941 tttcttaggt cctttgaccg cacagctact cgaggaattc aaagacgaga tcaaacaatt
    33001 tcggatcatg gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc agcacagtga
    33061 gatcggcatt atttatatga atgagcataa tcaagctggt atcagaagat atttacaaca
    33121 aggagagcta agtggccgca gcattggaaa attcaaaacg catatttttt taaggcaagg
    33181 acatccatta gctgaacacg cagagatcac caaagaagaa ttgttatctt atcctcaagt
    33241 tcgttttaca caggatggaa ataatttccc gtatttttat gaagacctta tcgagatccc
    33301 tgatcaagaa acagtgattt atacgagtga tcgaggtact ttgatgaata tcgttcttga
    33361 aactgatgct tacgcttcag gatctggtat tgtgattgga gggattcgcg agcacttgcg
    33421 gttaatccct ttggcagaag gcgaactaaa tgaattttat atcatttatt cggcgaaacg
    33481 tccgctcagt gaaatcgctc aaagatttat caaagagctt actcggttac tggaccagca
    33541 gagctaagaa aagcagatga gaacgctgtt tattgaacac cgttgatcaa aaaagagggg
    33601 ttgtgacaga attttgtcac aactcctctt tttgtagcaa acaatttata actaattgct
    33661 gttgtgggaa agtacgcaaa tatgattcta gaaacactca ttgattacct gttttgtttc
    33721 atagctgtaa ttttttcttc ttctggtgtc acgataatcg tcttttggtt ttcataaata
    33781 acataaccag gcttggcgcc atttggtttt cggatatgct ttacctgtac aagatcaacc
    33841 ggcacttggg cagagtatcg gtattttgaa aaataggcag ctaactcggc agcttctgta
    33901 attgtttcat ctgagggctt gtcactttta atgatgacgt gcgaaccggg tatatttttt
    33961 gcatgaagcc aatagtcggt ttttttagct gtcttcattg tcagctggtc attttgaaga
    34021 ttgtttttac caacaagaat gagcgttccg tcagaagaaa agtattgatc aggctgagat
    34081 ggtttcttac gtttttgttt ttttaagttc ttctttttca ggtatccttc tgaagtcaat
    34141 tcttctttaa ttgcttcgat atccattggg ccggcaattt ccagttgaga taagacagat
    34201 tcaagatatt ggatttcatc tttggcttct tggatttgtt cgccaatcag cttaacagcg
    34261 ttcttcagct tttggtatcg atggaaatat ttctgtgcat tttgatttgg tgtcaatgcc
    34321 ggatctagtg ctattttgat tggccgatct tcttcataat aattaggtaa aacaacttct
    34381 tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt tctacggtaa
    34441 ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt ttttcaattt atttttattt
    34501 cgttttagtt cattttcgat ttttcgaatc agttctccgc cttgttgttt tgcccgatct
    34561 ttctctgctt tttcgtgata atagctatct aataattgac tgagtgttgg ataagctgtt
    34621 acagcagatg cctgttcgga caatacctga taagcaatcg gtgtgaaaaa atctttattt
    34681 tccgtttcgt aaaaagtagg aataggctga tggctgatgg ctgagaaaaa ttcgtcccat
    34741 acaaccatct tttcattagg acgttccagt aatcttttgg tcagctcttg agcgatatcg
    34801 aaaccgattc cctgaaactg acgttggatt gcttttggat ctagttcagt ttgggaaagc
    34861 cgctgaaaga ttttttcttt ctcggagcta aatgggttca agacctcttg ttttggcgga
    34921 gcaacatatt ctacaccagg aagcaaagaa cgataagtgt tttgagagct tccaatgtgt
    34981 ttgatcgcat ccagtatttt tccagtttct cgattaacta agacgatcgt actatgacgt
    35041 cccattaatt caacgatcaa tatgatattt tgtagatcac ccaactcgtt tcgtttagca
    35101 aagtggaaat ggatcactcg atcattttcg atttgttcga tgtcttctaa aatagcgcca
    35161 tccaaatatt ttcgaagcat catcacgaag ttaggcggat tatcaggatt ctgataatcg
    35221 atttgggtaa tctgtacacg agcataactt gggtgcgcag ataataataa tcgatggttt
    35281 ttgccgcgtg aacgaatcac cagcacgatt tcattttcat aaggctgatg gattttcgag
    35341 atccggccag atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt aaatacacca
    35401 tcaaaggaca tggttcattc ctcatttctt ttcataagta tattcattat aacgaaaaaa
    35461 agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc ttggactaat
    35521 tacataaaaa acagtgtttc tatcgttgaa atccaaattt ttaggtataa taaaaaatag
    35581 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
    35641 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
    35701 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
    35761 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
    35821 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
    35881 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
    35941 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
    36001 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
    36061 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
    36121 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
    36181 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
    36241 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
    36301 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
    36361 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
    36421 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
    36481 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
    36541 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
    36601 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
    36661 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
    36721 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
    36781 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
    36841 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
    36901 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
    36961 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
    37021 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
    37081 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
    37141 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
    37201 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
    37261 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
    37321 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
    37381 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
    37441 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
    37501 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
    37561 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
    37621 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
    37681 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
    37741 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
    37801 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
    37861 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
    37921 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
    37981 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
    38041 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
    38101 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
    38161 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
    38221 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
    38281 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
    38341 gggattggtg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
    38401 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
    38461 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
    38521 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
    38581 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
    38641 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
    38701 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
    38761 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
    38821 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
    38881 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
    38941 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
    39001 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
    39061 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
    39121 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
    39181 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
    39241 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
    39301 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
    39361 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
    39421 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
    39481 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
    39541 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
    39601 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
    39661 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
    39721 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
    39781 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
    39841 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
    39901 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
    39961 taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
    40021 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagcagc
    40081 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
    40141 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
    40201 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
    40261 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
    40321 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
    40381 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
    40441 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
    40501 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
    40561 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
    40621 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
    40681 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
    40741 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
    40801 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
    40861 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
    40921 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
    40981 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
    41041 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
    41101 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
    41161 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
    41221 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
    41281 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
    41341 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
    41401 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
    41461 agatattaaa aaagggggcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
    41521 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
    41581 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
    41641 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
    41701 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
    41761 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
    41821 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
    41881 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
    41941 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
    42001 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
    42061 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
    42121 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
    42181 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
    42241 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
    42301 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
    42361 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
    42421 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
    42481 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
    42541 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
    42601 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
    42661 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
    42721 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
    42781 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
    42841 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
    42901 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
    42961 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
    43021 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
    43081 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
    43141 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
    43201 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
    43261 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
    43321 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
    43381 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
    43441 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
    43501 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
    43561 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
    43621 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
    43681 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
    43741 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
    43801 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
    43861 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
    43921 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
    43981 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
    44041 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
    44101 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
    44161 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
    44221 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
    44281 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
    44341 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
    44401 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
    44461 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
    44521 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
    44581 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
    44641 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
    44701 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
    44761 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
    44821 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
    44881 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
    44941 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
    45001 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
    45061 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
    45121 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
    45181 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
    45241 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa
    45301 gcaaaataaa agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat
    45361 agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag
    45421 aaaatctggc aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt
    45481 gcagacaggt gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa
    45541 agcacaagaa gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga
    45601 cgcgttaaaa gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc
    45661 aacgcgaaag ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    45721 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    45781 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    45841 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    45901 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    45961 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    46021 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    46081 tttttttatg tttaatcata caaaatagtt ttttcataaa aatatggaaa atatggatta
    46141 attttgaaat aattgattta aaaggaatac aaaagagtga atagtaggaa atagtggttt
    46201 agaactagtg ggctgatttt ttatatattt tagtttacat aatatatatt atacaaagta
    46261 gatttaaaaa atgataaacc tctctcgaac ggttttttat caaaaatcgt catttttcca
    46321 tcaaaatctg cttatgcaaa attataaaaa aattttgcag tttttcttac tttttttgtc
    46381 gtttaattga atgataaaaa ccagtttctt tattcagtta atcaaatatg aacgaattag
    46441 atatgaatca ttgtatttgc cgttgcggaa ggatttttta attaaccaca gataaacatt
    46501 gataacgatt cgcttattgt aatcaacaga ttaaatttca tatgttcata tgtacggata
    46561 cattttcgga ccgattaatt ttaaccattc tgtttatttg cttacctatt agatatcaat
    46621 aaaaaactgc ttaacagcta tttcagctgt taagcagttt taggattttt agttcatatt
    46681 cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg atttgctgaa
    46741 cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac cattttcgtc
    46801 aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag ttaaataagc
    46861 agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac gtgcagtact
    46921 cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag cagcattacg
    46981 gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt tttctacaat
    47041 ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt ctgaatcacc
    47101 atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt tcaatgcgat
    47161 ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac gtgatgttgg
    47221 cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg aagcaacaac
    47281 gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca tgattgacgc
    47341 atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac cagctgtgat
    47401 aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag ccacattttc
    47461 atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt
    47521 cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat aagcgatact
    47581 tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt tcatgaataa
    47641 acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg tttattcatt
    47701 ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac gaattcctga
    47761 atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa tacaaccaaa
    47821 agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata agacagcaat
    47881 cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct taatgtttat
    47941 ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat aaagaagtcg
    48001 tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt tcttgaatca
    48061 gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa gcatcaaaat
    48121 acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct gctgtttctt
    48181 ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc atctcatttt
    48241 ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc aattcgatca
    48301 gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta tcaaaataaa
    48361 tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg cgaaaatgaa
    48421 tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa gaaagtagat
    48481 tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct tttaataaga
    48541 tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct tctttatcaa
    48601 tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt tgtaaacatg
    48661 taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga acttccgcat
    48721 atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct cggtggattt
    48781 ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt ccttgttcac
    48841 gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc agctagttcc
    48901 aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg ctgtactgct
    48961 attatatgca aagctatttt tggtttcata aagactttct atggttggga catgaacttc
    49021 ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata agcgcttaca
    49081 aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg ctgtcagtct
    49141 ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg tcagctactt
    49201 atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct tttatggtca
    49261 ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa caaactgtga
    49321 tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca caaagttaca
    49381 gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc aattctttgg
    49441 cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg attggttttc
    49501 aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga cagctagtac
    49561 agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt cttgtcttgt
    49621 ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc acagccccaa
    49681 caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt ctcttgctgg
    49741 tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg acttattctc
    49801 taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca agtatggctg
    49861 ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt acactttctt
    49921 tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg tatgtgattg
    49981 ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac ttattcaacc
    50041 aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc ttattggttg
    50101 gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct tttgcttctc
    50161 ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg attagcgcgc
    50221 ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga gtagatgaag
    50281 aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg
    50341 aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc catggactgc
    50401 tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg tacatggatg
    50461 attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct tccatgttta
    50521 aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat ccatgtggaa
    50581 aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga catacatcag
    50641 ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc tggacagcaa
    50701 gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat atcgcaaaag
    50761 atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa acggagattg
    50821 gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc
    50881 tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta gaagctatgg
    50941 atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac
    51001 gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa cttcaagttt
    51061 ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct tctttactga
    51121 atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg ttttttatcg
    51181 acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca ttcatgatca
    51241 atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca ttaagaggac
    51301 aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg tggttgattg
    51361 gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc gtctacccta
    51421 agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg tttatttatg
    51481 ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa ggaacgcttg
    51541 ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc ggagatatct
    51601 tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa gaaaaatatc
    51661 aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc gcttttatcg
    51721 attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc gctgttttat
    51781 tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta ttgaatgaca
    51841 ctatcacaca gattactact ccatatttca aattctatga acaagatgct ttatgcaaac
    51901 tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg atgttatctc
    51961 atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat gaaaagtaca
    52021 gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca agcccaatct
    52081 atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat gaagaattta
    52141 tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct atcaaagatg
    52201 gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat agaacaggcg
    52261 gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca cttgtcttaa
    52321 caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat cagaagactt
    52381 taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt aattctgttc
    52441 tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata cgacaagtaa
    52501 aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta gtttcccaag
    52561 tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga ttttaagatc
    52621 tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat tgaaagcaga
    52681 acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag aagcagctcc
    52741 gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc ctatcatagc
    52801 aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata cagcattagg
    52861 tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta cgagtttttt
    52921 agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc cttgaaggac
    52981 gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta cagcgttgtc
    53041 tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa gtgcgacata
    53101 tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc tgaaactttt
    53161 aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc cgatccatcc
    53221 actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt ttccagtgat
    53281 gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc cttcgaagac
    53341 cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta aaatgactgc
    53401 tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt tatatcttgt
    53461 gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag cagtaggtgt
    53521 accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta ctccttgagt
    53581 atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga ttaaaccagc
    53641 accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga tcacttctga
    53701 aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga ggatggaggg
    53761 ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt cctcccttga
    53821 atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct tcgctgtcaa
    53881 tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta tctttcaaag
    53941 aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca tccgtatgac
    54001 ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg atgttcatct
    54061 ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc atatcattcg
    54121 tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa taaatcttga
    54181 agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag atgtgcctca
    54241 ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt atcaagcaga
    54301 tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc atcttctgga
    54361 cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata ctgctccacg
    54421 cgctcgatca tagctttgat ataactttct tctattttat tttctgataa tagcggctgt
    54481 gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg gatcatttct
    54541 tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa aagtttgttc
    54601 attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt taattcgata
    54661 taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc tggtatcagc
    54721 caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat gattggatgc
    54781 agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga aaaaataata
    54841 tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag
    54901 atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct aatgccactt
    54961 ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc ttttctgatc
    55021 tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct cttccctatt
    55081 cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc atattgatgc
    55141 ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg aaaataggca
    55201 gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa ttctacggcc
    55261 atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa atcaagtgca
    55321 ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa tataatctcc
    55381 gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat gtcagtgact
    55441 gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt tccaattcga
    55501 atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc gaatcgaaga
    55561 tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata atcaatggaa
    55621 agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat caaacgagca
    55681 gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct aaaaccagtt
    55741 ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat ctttttgata
    55801 tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa aacagaaaca
    55861 gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg ttcggtatac
    55921 acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc tcggaatttc
    55981 tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg tttgatcgcc
    56041 tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc tcttggtgtt
    56101 ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt atattctagt
    56161 atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat tttttttacg
    56221 actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac caaatgtctc
    56281 ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt ttgaggtcag
    56341 ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt caaagtgatt
    56401 gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag ctgtttcttt
    56461 attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt ctgtttcacc
    56521 agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg aacctcttaa
    56581 tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta gttctatact
    56641 ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc ttacaaatat
    56701 gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa attttcattt
    56761 gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat gtctcgtaaa
    56821 tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct tcatgataac
    56881 tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg ataccaaagt
    56941 cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct tttgtttgtt
    57001 tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg atgcggaaat
    57061 aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg aagtaatcat
    57121 tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct ggttcgccga
    57181 aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag ctgttcgcag
    57241 tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa tgcatttcta
    57301 cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc aagaagtcag
    57361 aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt gctaatgcaa
    57421 ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt acattcttac
    57481 catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta gtgatgacaa
    57541 ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat tcgatgtctt
    57601 cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg attttgttcg
    57661 taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg ccaagataag
    57721 cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc ctagaaaagc
    57781 gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac ttgttatcat
    57841 tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata gtagcggaac
    57901 caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga agaaaaacta
    57961 acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat ccgtaggaat
    58021 atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta tacttcccat
    58081 tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga attccgtttc
    58141 gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat tgatcctaat
    58201 tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc ggatttatcg
    58261 atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga tcaaatcggt
    58321 aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg
    58381 gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat ggctgaagaa
    58441 tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc catttttatt
    58501 agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa gcatgtagag
    58561 gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca ggctgatatg
    58621 tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt ctcctctcaa
    58681 attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca aaattttgaa
    58741 gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa
    58801 tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg tttgataggc
    58861 tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct cagcctgttt
    58921 gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa cttctgtttc
    58981 ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt atacatgcag
    59041 ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact tctttttgaa
    59101 cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga ctgttgacca
    59161 tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt ctcagatctt
    59221 ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt gtccgtccat
    59281 tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg aacaactttg
    59341 cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc cccgtaggat
    59401 aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc gtttcatcaa
    59461 aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta tgtttctcaa
    59521 aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct gcacctggat
    59581 ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg attttagcca
    59641 tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc tgctgatcca
    59701 gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt cctgagagaa
    59761 gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt tgtgttctcc
    59821 ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg ctgctccttt
    59881 ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca
    59941 gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt accgcctgtt
    60001 gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa ctgtcctgct
    60061 gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg aacatatttt
    60121 tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt cacatcgatt
    60181 aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac ccagctgacg
    60241 gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg atcacgaacg
    60301 acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac tttaacaaat
    60361 cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt atgcccgtta
    60421 tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt caaatgcata
    60481 tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt cggatcaact
    60541 gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc gatttcttta
    60601 tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa tgttaaagca
    60661 gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca
    60721 gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc gattgcgaca
    60781 tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt tttgatggtc
    60841 ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt ttgcttttca
    60901 atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc aacttctaaa
    60961 tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt gttgccgatg
    61021 tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt ccaagtgtac
    61081 aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc ttctttagaa
    61141 ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt tgtaactttc
    61201 aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc tagttcttct
    61261 gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg gaaagctaaa
    61321 accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt gtagactgca
    61381 tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc atctgtcgtt
    61441 cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc tgcatccacg
    61501 gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac tgaaaatgaa
    61561 atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct gttttggatt
    61621 aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc tgctgtctcc
    61681 tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat aaggaaaagg
    61741 actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct gttctttttt
    61801 tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct aaaagtgtat
    61861 ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta ttgactactt
    61921 tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta aacggaattt
    61981 ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga taacttcctg
    62041 ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga agcgcatctg
    62101 attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt cctgctttgt
    62161 ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc tcgattttga
    62221 caaattgctt actccctgta tatacagaga ctttctttgt actgacacca aaaccaaaat
    62281 ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt agctgcatct
    62341 gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc gaaaataaaa
    62401 catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt tctccattcg
    62461 atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt
    62521 cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa taagtgattt
    62581 tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa ggtggatcag
    62641 ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt tgattgtttt
    62701 gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg acttctggta
    62761 ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta gcggccagag
    62821 aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca
    62881 taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc gttttgccct
    62941 tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg acaccattta
    63001 atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga tttttcggca
    63061 gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct gtttctgttt
    63121 ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac atactaagcc
    63181 aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa gataccaaat
    63241 agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa ttcctcccgt
    63301 ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt tgtttttggc
    63361 tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc catcttcgtg
    63421 tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa aagtttcggt
    63481 cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt cttgtccatc
    63541 ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc cttttaatgt
    63601 ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc gattttgatg
    63661 agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc
    63721 atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg caggaccagc
    63781 tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa gacctttttc
    63841 attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt tctgtaggga
    63901 aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat acttacgtac
    63961 tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat catcggaagt
    64021 agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga tttcccaagt
    64081 agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg tataggtcat
    64141 ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat gatctgttag
    64201 taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat ccttttcacc
    64261 tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag tatttttcca
    64321 tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt tgattgtttg
    64381 gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct cttgataact
    64441 ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc
    64501 tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg
    64561 ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt tgtcaaattc
    64621 ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg gtataccgaa
    64681 ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt tcatttgata
    64741 ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt aatggatttg
    64801 gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc cttctatgga
    64861 aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa gtgtaccagg
    64921 ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta cagttgctga
    64981 gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat cagctgattc
    65041 atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag actctacttc
    65101 tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac catgtatttc
    65161 gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg tactgtcgat
    65221 tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta gagaagtatt
    65281 tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc ttgattgtgc
    65341 gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca aaacgatcac
    65401 ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg ctttttgtgt
    65461 aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt tcactgaatc
    65521 aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg agtagccgac
    65581 atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga caaaacgatc
    65641 gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg accagtctac
    65701 gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag cattcaaata
    65761 aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg catagtctgc
    65821 ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag gatctccatt
    65881 ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat gatcaagaac
    65941 atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat catctgttgt
    66001 atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg ataatgtagg
    66061 caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt ctatttcgtc
    66121 tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa taatgtcata
    66181 ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg
    66241 aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct
    66301 atttacctga taagcatagc catctccttc ttttctttcc acatcactta agtaattttc
    66361 gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat ttagcttgat
    66421 gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg tttctaaaac
    66481 tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg ataaactcaa
    66541 tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt ttcccctcct
    66601 ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac aggcgttaca
    66661 taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg gaatcacacc
    66721 aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat gagcgtttca
    66781 ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaac gaaactatag
    66841 aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga ctaaaaaaga
    66901 acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg aagagatcaa
    66961 aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt atcgtcttga
    67021 acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa
    67081 cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt attattgtga
    67141 aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca atcaaatttt
    67201 agaaagctat tta
//
LOCUS       DACERL010000006        64960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.006, whole genome shotgun sequence.
ACCESSION   DACERL010000006 DACERL010000000
VERSION     DACERL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64960
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.006"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(60..392)
                     /locus_tag="IXF54_000380"
     CDS             complement(60..392)
                     /locus_tag="IXF54_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1403665.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(444..1073)
                     /locus_tag="IXF54_000381"
     CDS             complement(444..1073)
                     /locus_tag="IXF54_000381"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1403666.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(1037..1873)
                     /gene="mutM"
                     /locus_tag="IXF54_000382"
     CDS             complement(1037..1873)
                     /gene="mutM"
                     /locus_tag="IXF54_000382"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1403667.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(1918..4563)
                     /gene="polA"
                     /locus_tag="IXF54_000383"
     CDS             complement(1918..4563)
                     /gene="polA"
                     /locus_tag="IXF54_000383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1403668.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(4689..5372)
                     /locus_tag="IXF54_000384"
     CDS             complement(4689..5372)
                     /locus_tag="IXF54_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1403669.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(5365..5850)
                     /locus_tag="IXF54_000385"
     CDS             complement(5365..5850)
                     /locus_tag="IXF54_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1403670.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(6157..7491)
                     /locus_tag="IXF54_000386"
     CDS             complement(6157..7491)
                     /locus_tag="IXF54_000386"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1403671.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(7622..8383)
                     /locus_tag="IXF54_000387"
     CDS             complement(7622..8383)
                     /locus_tag="IXF54_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1403672.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(8399..8881)
                     /locus_tag="IXF54_000388"
     CDS             complement(8399..8881)
                     /locus_tag="IXF54_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1403673.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(8901..11081)
                     /locus_tag="IXF54_000389"
     CDS             complement(8901..11081)
                     /locus_tag="IXF54_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1403674.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(11225..11956)
                     /locus_tag="IXF54_000390"
     CDS             complement(11225..11956)
                     /locus_tag="IXF54_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1403675.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(12025..12966)
                     /locus_tag="IXF54_000391"
     CDS             complement(12025..12966)
                     /locus_tag="IXF54_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1403676.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(13146..13514)
                     /locus_tag="IXF54_000392"
     CDS             complement(13146..13514)
                     /locus_tag="IXF54_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1403677.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(14125..15516)
                     /locus_tag="IXF54_000393"
     CDS             complement(14125..15516)
                     /locus_tag="IXF54_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1403678.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(15729..16115)
                     /locus_tag="IXF54_000394"
     CDS             complement(15729..16115)
                     /locus_tag="IXF54_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1403679.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(16171..16794)
                     /locus_tag="IXF54_000395"
     CDS             complement(16171..16794)
                     /locus_tag="IXF54_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1403680.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(16858..17526)
                     /locus_tag="IXF54_000396"
     CDS             complement(16858..17526)
                     /locus_tag="IXF54_000396"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1403681.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(17538..17978)
                     /locus_tag="IXF54_000397"
     CDS             complement(17538..17978)
                     /locus_tag="IXF54_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403682.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(18037..18708)
                     /locus_tag="IXF54_000398"
     CDS             complement(18037..18708)
                     /locus_tag="IXF54_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1403683.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(18893..19291)
                     /locus_tag="IXF54_000399"
     CDS             complement(18893..19291)
                     /locus_tag="IXF54_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1403684.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(19288..20061)
                     /locus_tag="IXF54_000400"
     CDS             complement(19288..20061)
                     /locus_tag="IXF54_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1403685.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(20058..20741)
                     /locus_tag="IXF54_000401"
     CDS             complement(20058..20741)
                     /locus_tag="IXF54_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1403686.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(20908..21795)
                     /locus_tag="IXF54_000402"
     CDS             complement(20908..21795)
                     /locus_tag="IXF54_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1403687.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(21792..22661)
                     /locus_tag="IXF54_000403"
     CDS             complement(21792..22661)
                     /locus_tag="IXF54_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1403688.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(22798..24084)
                     /locus_tag="IXF54_000404"
     CDS             complement(22798..24084)
                     /locus_tag="IXF54_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1403689.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            24357..24935
                     /locus_tag="IXF54_000405"
     CDS             24357..24935
                     /locus_tag="IXF54_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1403690.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            24935..25864
                     /locus_tag="IXF54_000406"
     CDS             24935..25864
                     /locus_tag="IXF54_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1403691.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            25861..27597
                     /locus_tag="IXF54_000407"
     CDS             25861..27597
                     /locus_tag="IXF54_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1403692.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(27660..28496)
                     /gene="pheA"
                     /locus_tag="IXF54_000408"
     CDS             complement(27660..28496)
                     /gene="pheA"
                     /locus_tag="IXF54_000408"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1403693.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(28493..29002)
                     /locus_tag="IXF54_000409"
     CDS             complement(28493..29002)
                     /locus_tag="IXF54_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1403694.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(28995..30290)
                     /gene="aroA"
                     /locus_tag="IXF54_000410"
     CDS             complement(28995..30290)
                     /gene="aroA"
                     /locus_tag="IXF54_000410"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1403695.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(30303..31394)
                     /locus_tag="IXF54_000411"
     CDS             complement(30303..31394)
                     /locus_tag="IXF54_000411"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1403696.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(31411..32577)
                     /gene="aroC"
                     /locus_tag="IXF54_000412"
     CDS             complement(31411..32577)
                     /gene="aroC"
                     /locus_tag="IXF54_000412"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1403697.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(32579..33643)
                     /gene="aroB"
                     /locus_tag="IXF54_000413"
     CDS             complement(32579..33643)
                     /gene="aroB"
                     /locus_tag="IXF54_000413"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1403698.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(33676..34698)
                     /gene="aroF"
                     /locus_tag="IXF54_000414"
     CDS             complement(33676..34698)
                     /gene="aroF"
                     /locus_tag="IXF54_000414"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1403699.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(35357..36331)
                     /locus_tag="IXF54_000415"
     CDS             complement(35357..36331)
                     /locus_tag="IXF54_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1403700.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(36312..38195)
                     /locus_tag="IXF54_000416"
     CDS             complement(36312..38195)
                     /locus_tag="IXF54_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1403701.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(38213..40438)
                     /locus_tag="IXF54_000417"
     CDS             complement(38213..40438)
                     /locus_tag="IXF54_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1403702.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            40553..41587
                     /locus_tag="IXF54_000418"
     CDS             40553..41587
                     /locus_tag="IXF54_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1403703.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(41654..42667)
                     /locus_tag="IXF54_000419"
     CDS             complement(41654..42667)
                     /locus_tag="IXF54_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1403704.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(42745..42933)
                     /locus_tag="IXF54_000420"
     CDS             complement(42745..42933)
                     /locus_tag="IXF54_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1403705.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(43373..43501)
                     /locus_tag="IXF54_000421"
     CDS             complement(43373..43501)
                     /locus_tag="IXF54_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1403706.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(43504..44877)
                     /gene="rlmD"
                     /locus_tag="IXF54_000422"
     CDS             complement(43504..44877)
                     /gene="rlmD"
                     /locus_tag="IXF54_000422"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1403707.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(44988..46043)
                     /locus_tag="IXF54_000423"
     CDS             complement(44988..46043)
                     /locus_tag="IXF54_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1403708.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(46053..47483)
                     /gene="gatB"
                     /locus_tag="IXF54_000424"
     CDS             complement(46053..47483)
                     /gene="gatB"
                     /locus_tag="IXF54_000424"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1403709.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(47480..48952)
                     /gene="gatA"
                     /locus_tag="IXF54_000425"
     CDS             complement(47480..48952)
                     /gene="gatA"
                     /locus_tag="IXF54_000425"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1403710.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(48952..49257)
                     /gene="gatC"
                     /locus_tag="IXF54_000426"
     CDS             complement(48952..49257)
                     /gene="gatC"
                     /locus_tag="IXF54_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1403711.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(49363..51399)
                     /gene="ligA"
                     /locus_tag="IXF54_000427"
     CDS             complement(49363..51399)
                     /gene="ligA"
                     /locus_tag="IXF54_000427"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1403712.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(51416..53650)
                     /gene="pcrA"
                     /locus_tag="IXF54_000428"
     CDS             complement(51416..53650)
                     /gene="pcrA"
                     /locus_tag="IXF54_000428"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1403713.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            53805..55364
                     /locus_tag="IXF54_000429"
     CDS             53805..55364
                     /locus_tag="IXF54_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1403714.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(55475..57406)
                     /locus_tag="IXF54_000430"
     CDS             complement(55475..57406)
                     /locus_tag="IXF54_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1403715.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(57424..58338)
                     /gene="pfkB"
                     /locus_tag="IXF54_000431"
     CDS             complement(57424..58338)
                     /gene="pfkB"
                     /locus_tag="IXF54_000431"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1403716.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(58335..59087)
                     /locus_tag="IXF54_000432"
     CDS             complement(58335..59087)
                     /locus_tag="IXF54_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1403717.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(59246..59455)
                     /locus_tag="IXF54_000433"
     CDS             complement(59246..59455)
                     /locus_tag="IXF54_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403718.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(59466..60794)
                     /locus_tag="IXF54_000434"
     CDS             complement(59466..60794)
                     /locus_tag="IXF54_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403719.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(61159..61746)
                     /locus_tag="IXF54_000435"
     CDS             complement(61159..61746)
                     /locus_tag="IXF54_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1403720.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(61829..63079)
                     /gene="clpX"
                     /locus_tag="IXF54_000436"
     CDS             complement(61829..63079)
                     /gene="clpX"
                     /locus_tag="IXF54_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1403721.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(63326..64615)
                     /locus_tag="IXF54_000437"
     CDS             complement(63326..64615)
                     /locus_tag="IXF54_000437"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1403722.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(64769..>64960)
                     /locus_tag="IXF54_000438"
     CDS             complement(64769..>64960)
                     /locus_tag="IXF54_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403723.1"
                     /translation="EEFTLQSAIVYPVYDTYIEDPKRWFQLTVEACTGKTMYEGTEDE
                     KQDFLKKREKILQQMIFFH"
BASE COUNT        17893 a        14077 c        11493 g        21497 t
ORIGIN      
        1 cttgaagcag atgaacacct tctcatctgc ttcaagtcac atctcgattt tttactattt
       61 tataacttct cttcgattcc tgtatagtgt ttcattcgct gttcctcgat tttttgctca
      121 tcggaaatgt ggtcactttc tgggagcggt gttttactac gatcaggtgt cggatttttt
      181 cgttcttgag tcaaatcttg ccgcttcgct tcttcttctg cctgctggtc ttctatttca
      241 tcgattcttg cttggtactg cttttctttt tcttctgctg tttctgattg cgtacgacct
      301 tcatcgatta gattttcttt attgagcatc tcgccgattt tttcttttgc gttacctacg
      361 actttatcct tttgattttt taatgatccc atcgctatcc ctcactttct atatactcag
      421 gatagcaaaa ttgacacaga tactcaaaga aagcgattag caaaaagcca attttcgatt
      481 tgtcccacaa gtttttcttt tgttccattg ttgtcgaaca aaatatccgc acgctgcttt
      541 ttttcttcga ttggaagttg actactaatt ctctgctgcg cttgttctag cgtcagttga
      601 ttacgtttca tcaaacgctg aagctgagtt tctggcgtag tataaacgac agctactgca
      661 tccatatagt ggtcataatg accttcgtag agcaatggga tatctacaat tactaaagga
      721 tgattggaac ttagagaggc gatttgatct tttatcgctt ttcggataaa cggatctaat
      781 agctgatcaa gtttttgccg tttttccttt gatggaaaaa caatcatccc aagtttccct
      841 cgatctagtt gcccatcagg ctgcaatacc tcttggccaa acactgatac aattttcctc
      901 agagcaagcg ttccaggctc aacagtttct ctggcaatca catcgccatc gatcaaagga
      961 aatccatgag actgaaagac tttggctgcc gtacttttac cggtagctat tccacccgtc
     1021 aaacctaaaa caaatcctac tttgcgattt ttagcctttg acaattcgga caatagtgcg
     1081 tccccctttg tgccactttt gttttcacga tcggcgttcc acaacgtata catggtttgc
     1141 cagtttgctg atagacatgc agtgctactt gaaatccgcc tgattctcct aatgcattca
     1201 aataactgcg aatcgtggta cctccggctt ctattgcacg atccaaaaca tcaatgatcg
     1261 atagacgtaa ttgttctacc tctttggggc gcagtgtgtt cgccggttgt tctggatgga
     1321 tcttcgcttc ccataatgct tcatctacat aaatattacc tagaccagtc actagacgtt
     1381 ggtctagcag caatggcttg attgctttgt gggacttttt cagtccattt gcgaaatctg
     1441 ctaataagaa gctatctggc aaaggctcag gtcccaattt catgattcct cggtaagttg
     1501 cacttgctcc tttttcgacg attgtcatac gaccaaattt tcgtacatcg ttgtagcgaa
     1561 gttggcttcc atcctcaaaa aagaaaagta catgcgtatg tttatcgatt ggtgtatttt
     1621 ctttcgtaaa ttgatatttt ccttccattc gcaagtgtga gatcagttca caatgatcga
     1681 gatgaaaaat taaaaacttc cctctacgtc caatggattg gatggtttcg ccaacaagag
     1741 aagcctcaaa gatgggtgtc tctggttgct cgatgatctt gggccacaag acctgaactt
     1801 tttgaatcgt ttttcctact actaaccgtt ccaatccttt acgaacggtt tctacttctg
     1861 gtaattctgg cattttttct tttcaccttc attatattgc tctgtcttaa acaatgttta
     1921 ttttgcttcg taccacgttt ttccccaact gctgtcagtg atcagtggta catgaagaga
     1981 aacagcttgt tccataactt ccttcaccaa tttatctaat gattcaagtt ctttttccgg
     2041 tacctcgaag acaagttcat catgaacttg taacagcatc gtagcttgaa gacctgtttc
     2101 ttttaatcgt ttatccaatt cgatcatcgc gattttcaaa atatcagcag cacttccttg
     2161 gattggtgta ttgatagctg ttcgttccgc aaaagaacgg atattgtaat ttcttgaatt
     2221 gatgtctggc agatatcgac ggcggtgata cagcgtttcg acgtagcctt ggtcttttgc
     2281 ttcgcgtacg attctttcca tatattcttt gacacctggg tatttttcaa agtacgtatc
     2341 gatgtattgc tgtgctgctt ttcggctgat tcctagattt tgtgaaagtc cgtaatcaga
     2401 gatgccatac acgataccaa aattcactgc ttttgcctgc ctacgcatat ttggtgtgac
     2461 atcctctgcc ttttcgattc caaatacacg catcgctgtg ctggcatgga tatcttgacc
     2521 ttctaggaag gcagctttca aatgttcatc atttgagata tgcgcaagta cgcgtaactc
     2581 gatttgtgaa tagtcagaag aataaatcag ccagtctttt tctctaggaa caaatgcctg
     2641 acgtatcttt ctcccttcat ctaatcgaat cgggatgttc tgcaaattag gatcaacaga
     2701 gcttaatcgt cctgtttggg tcaatgtctg gacataacgg gtatgcactt ttccatctga
     2761 ttggatcact ttcagcagtc cctcgacata ggtagattga atttttgcaa tttgacgata
     2821 ggtgaggata tcctctacga ttggtgcttg ttcacgaagc tgttccagta catctacggc
     2881 tgtggaatat ccagtttttg tttttttgat aacgggtaat cccatttttt caaataagat
     2941 cactcctaat tgctttggtg aattcaaatt gaattcttcg ccggcttctt cgtagatttt
     3001 ttgttcgatc tctcttaagc gagcagagaa ctcgcccttc atttcttgta agcgagtcgc
     3061 atctactttg atcccgctta tttccatctc tgccaaaatg atagacaatg gcagttccat
     3121 tttccggaaa agatcttctt ggtttttatc tgccagttct tggctcaatt ttggtgtcaa
     3181 cgcattgatt gcagctactt ttcgcgctaa atgagcaaaa aagagttcct cttcttctgg
     3241 taaacctttt ttcgcccctt tgccgtatac agcttcatct gattggatat cgttgtatcc
     3301 gtaatgagca gctattcctt cgatatctgt gcttttttca ttcgtatcga gaaggtatgc
     3361 acctagcaag acatcaaaat cgattccttt cgtttttcca gtatagcggt ttaaagcaac
     3421 ataagttcgt ttcgcatcat atactttttt cagccgagtc ggatcagtga tccaagaatg
     3481 gaacgctcgg ttttcgaata cagatagatc gttcgtgaca taaattttct tttctgtccc
     3541 ccacgctact ccgacgattt cctctgtatg ataattgtcg cccatcattt ccacgtacaa
     3601 cgccatatcc gtagtaaaca tttcctcttt gaattcatga acgacttcaa acaaaatatc
     3661 atccattttt acttcttctt ctacgatatt caatttagaa aggaactgat tgaaatccat
     3721 ttctttataa aacggaacga gtttatcgag atttttccct tcatacttca atgagtcgat
     3781 agagacatcc acaggcgctg aagtattgat ggtcgccaat tttttcgata agaaagcctg
     3841 ctctttgtca tttatcagat tttctttcat tttgctttgc ttcatttcat cgatatgttc
     3901 ataaattcct tcgactgagc catattgttt caatagcttg atagctgttt tttcaccgat
     3961 tttagtcaca ccggggatat tgtcagaggc atctccggct agtcccttca tgtcaataat
     4021 ctgttttggc gtcaacccat catatttttc cgctacatgt tctggtgtat agctttcgat
     4081 atcgctgaca ccttttacag tgatatccac ttttacttct ttactagcta attgagtcaa
     4141 atcccgatca cctgaaagca cgacgacatc aaattgatct ttgtctactt tttctgccaa
     4201 agtaccgata atatcatctg cttcataatt gggcaattcg taatactgta cccctagtcc
     4261 tgtcaacaaa tcacgtatat atggcatttg ttctttgaat tcgccaggtg ttttggatct
     4321 tccacctttg tactccgcat acatttctgt ccgaaaagtc gtttttcctg catcgaacgc
     4381 aaccagaaca tgagtcggtt gctctttttg cataacattt tcaaacatcg tattgaatgc
     4441 atagattgca tttgtatgaa gaccgtttct atttttgaaa cgttcaagag aattgtgaag
     4501 cgcaaagaat gcgcgaaaag ccacactgtt cccatcaacg agtaataatt tattttttgt
     4561 catcttctta actccttact gtaaagttct tattcattct aacaaagaat cctgcggaat
     4621 gcgaaaagaa aaagcgggac cataccaaat aattcggtat agccccaact gatgaatttt
     4681 tttctttttt agttgttccg tccaaagatt ctcagaaacg ccaagaacaa gttgataaag
     4741 tccaaataca gcttcaatgc catgaataca gcaattccac tatgtgctgt tccgtttgtc
     4801 tggacataga tattacggat gctttggtta tcataagctg taatccctgc aaagatgacg
     4861 actaacagta gggagatgaa cagctcaact acgctacttc tcaaaatgaa gatgttcaag
     4921 actagtgcaa tgatcactcc gattaaagct gcctgtcctg ctcttccaac cccgctaaga
     4981 tctctttttg ttgttactcc gacaacagac atccccatga acatcaaagt agctgtcaag
     5041 aaagcttgcg ttactgttcc aatatcgtag tatgcaagag taaccgctaa tgttatgcca
     5101 ttcaaaagag agtaggcaat aaatccaccg attgctaagc tcggattctt ttgtgctttt
     5161 acgcctagaa ccattactaa aatcagttca acgatccaca gacccacaaa gcctagtgga
     5221 aaattattga taaaagttgc taattgcaaa ggaaataccc aaagagccaa gtaagaagtg
     5281 actgcactaa gggcaagtcc catacctaaa aatccataaa cttttgcata aaaacgattg
     5341 atacctgaca ttgatgtact tggattattc attcaattca ccttcacttt cgattgctat
     5401 ttccaattca ggaacttgtg gcggttggac cataacgaca gcatccaaca cacgatcttc
     5461 gttcgtattc tccgcttcta cagaaatcgt caccacatcc ttcatcttat ctacttttat
     5521 gacctcaaac tgaaaagtca gtgtttcata atggaaaacc ggttcaataa aattgacaga
     5581 aaaattgaca acatgtgatc ctggtccagg aagatgcttt gaaattgcac ttgtgatgat
     5641 ccccatcagc atcactgatg gaacgattgg ctgttgatat tctgtttttt gtgcaaagtc
     5701 atgttggata tacaaaggat ttgcatcatt cgtcaatccc aaatacagca aaagctggtt
     5761 atcttcaata gattctgtta aggataacga gtcgccttcc tcgatttcat ttattgtttt
     5821 tcctagtttt ctcggcttac ctatgttcaa gatagaaacc ctccagttta tttttaatca
     5881 tacattattg taccaaaagt agcgtaaaag gcaagttctg ctcccttgat ttaacagatt
     5941 cctatagttt acagaggtcg tgaaaaagct gtcctgcatc aaataataag aacagccaag
     6001 aaaaaacagc ttctcatgtt tttgatttga ttgggcttat taccgcagat gcaagctttt
     6061 gaacaccgtt tatcataagt tgcaaaaaag ggggcttggc aagatttttg ccactccccc
     6121 tctttctatt tttataagga cactgcagta attgtattac agtacatttt tcgtcgtgct
     6181 gcttaaaagt ttttcatacg cttgttcaaa tttttgaaca tctccagcac ccatgaagat
     6241 tactactgca tcatgataat ctaacaatgg agatacatta ttttctttga tgacttctcc
     6301 accttttttg attttcgctc caaggtcttc aatcttaacg tttccttgtt cttcccgagc
     6361 cgaaccaaaa atatcgcata agtaaacttt atcagctaaa tcaagtgctt ctgcaaattc
     6421 atccatcaat gcaatcgtac gcgtaaacgt atggggttgg aaaactgcaa ttatttcttt
     6481 atctggatat ttttggcgtg cgccatcaat cgttgctttg atctctgcag ggtgatgcgc
     6541 ataatcatcg actacggtca tgtctgctac gatcttttca ctgaatcgac gttttactcc
     6601 tgggaaagtc agcatttctt ctgcaacttc tttcagatcc agtttttcga aataagctac
     6661 tgcaataaca cctaaagcat ttaagatatt gtgtttaccg aatgctggta cagtgaagtg
     6721 cccaacaaat tcatccccat gatagacatc aaaagcagaa ccagaagttg tgcgttcgat
     6781 gtttcgtgct tggatatcat cgttttctgt gacaccataa taataaatcg gcacatttgc
     6841 ttttaatttg cgtaaataag catcgtcacc atacgcaaaa atagcttttt tcacttgtcc
     6901 agccattgtt tggaaagctg taaatacatc gtcgatactt gtataataat ccggatgatc
     6961 aaaatcgata ttggtcatga ttgcatagtc tggtgaatag gcaaggaaat gacggcgata
     7021 ttcacaggcc tcaaatgaaa agaattccgc ttgcggatca ccatgtcctg ttccatctcc
     7081 aattagataa cttgttggac ggacaccact caatacatga gagagcagtc cagtcgtact
     7141 tgtttttccg tgagagcctg ttacagcgat actcgtataa ttttggataa aatgaccaat
     7201 aaagtcgtga tagcgaatca cttccaaacc taattctttt gctcgctgga tttcttcatg
     7261 tgaatcagga aatgcatttc ctgcaatgat cgtcatccct ggttttacat tatctgcgtt
     7321 aaatggcaga atcgtgatat ttgctttttc taaatctctt tgtgtaaaga aatatttttc
     7381 aatgtctgat ccttggacat ttagaccttg ttcatgtaaa acaagagcca aggagctcat
     7441 tcctgaacct ttgataccaa caaaatgata caatttattt tgattttcca tttgaatcct
     7501 cttctcttat ctagcgtcca ttcttttagc tattacattc aacacgctcc tcattatctt
     7561 ataaacgatt gaaaatttca accaaattta tttggaaagg aggattcgct tgctaaaatg
     7621 gtcaagtacg agaaaaatcc gtttttattg actctttaat tctggtgtaa gaagaccaaa
     7681 cttcctctga tatatcaatg tgaggaataa aatcttttcc tttcggacca tagccgttct
     7741 catcgtcatg gagacgttcc atttctccta atactagcgc atggaaatat tccttaggcc
     7801 attgatgatt cagttctttt gctgtaaaga tgatctcttc agaaaagtga aggatttccg
     7861 gcacaaatgg cacagcattc gcgaaaatag tttgttcatt ttgccatact ttctcaaaag
     7921 ttgctcgagt cctgcgttgc agtgtcggat cattcatcaa taagattttt tctggtacag
     7981 ctcctaagga atgaagaatc tccagtgaaa aagtggcgtt ctctccagaa tttgtagact
     8041 tcgattctat cagaaaagcc ttatcgggca aatcatagac ttcttttaga tactgacgac
     8101 agatttcact ttcactcatg ccatcttcaa aatgaaatcc ttgcttttca aaattctcat
     8161 aaaggattct agtcgcatgc cccacccctc cgaccaaaaa caattgatct acttggccat
     8221 ttaaatacat ttgtgctgct ttatcagcta agataggaag acagtttcca gctaaaataa
     8281 gtgtaggtgc cgtcagttcc tcaaaatttc cctcttcagg tgcggctaaa aaatttaata
     8341 gctgattcca gtcttttaga ttcctctgct cacttttttc caaaggcctt ctatcctttc
     8401 atctgatttt ttgtgcttca ttaaagtctt ctctttttgt ctcaatccat ttgatttctc
     8461 catgacatct tccacaacga tatttgcgta catcgattct tctcttgcgg cagatcgtgg
     8521 cagaacaatc ttggcatcga taatactcca tctttatctg tttcttcgtc aaggctggtg
     8581 catagcgcaa ccctcctgtt tttttcaaaa gatacttgaa atccatatct ttatgctgat
     8641 accctcttcc agctagatga agatgataat ggcaaagttc atgtttgatg attcctgcaa
     8701 tcgtctcttc gtctgactct tcaaatagct tcgcattaaa atctaaatga tgatcgttta
     8761 aatgatagcg cccgccagtt gtacgtaagc gtggattgaa cattgcttgg tgcttgaaag
     8821 gaagctggaa gctttttaat gatatctctt ctactaactg ctgcagttct tcttgtgtca
     8881 atttttgtta ctcctcattg ttactcttca taaggggaaa gcatcgtcaa actgattctg
     8941 ccttttttcg tatcgacttg ttctatccaa actgttacga tatctcctac tgacacgaca
     9001 tctgtaggat gcttgacgaa ttttttgctg agtttcgata tatggacaag tccgtcttgc
     9061 ttcacaccga tatcaacaaa tgcgccgaag tcaatgacat tacggaccgt tccagtcaat
     9121 tccatccctg gtttcaagtc ttccatactc aatacatctg tacgtaataa gggcgctggc
     9181 atctcatcac gcatatctcg tccaggttgt gtcagcccag ctaaaatatc ggctaacgtt
     9241 tcttctccga ctgataggct ttcagccagt ttctctacgg ataatcgtgt caatttttct
     9301 actgcttctt ctgtaccaag ctctttttct gaaagttgga ctgaagttaa tatttctttt
     9361 gcaatagaat aactttcggg atggatacct gtgttgtcca aaatgttttt cccaccaggg
     9421 actcgcaaga atccgattgc ttgttcatat gcttttggtc ctaagcgtgg cacttttttc
     9481 aattgagttc gtgcggtgaa ttctccgttt tcttcacgat aaatgacaat gttttgggca
     9541 gttgttttat tcaatcctga tatatgctgc aagagctgcg ggcttgctgt attcacatcc
     9601 acacctactt ggttaaccgc tgtttccaca acaaaatcca attgttcagc caaacgtttt
     9661 tgagaaacgt catgttggta ctggcctact ccgacagctt taggatcgat tttgactaat
     9721 tcagctaaag gatcttgcag gcgacgggca atagaaacag cacttctttc ttcaacttgt
     9781 agatgcggaa attcttttcg tgcaatttcg ctagcggagt aaacagaagc gcctgcttcg
     9841 ttgacgatag cgtaataggt tttatggtcg gcggatttca actgctcagc cacaaaaagt
     9901 tcagattccc gactggctgt accatttccg atagctacca tatctacttg atatttatta
     9961 attaattgga taaaagctgg tccagcagct tctctttttg cttgcgctgc aggtttatga
    10021 ggataaataa cttcgattgc taaaactttc cctgtcgcat ctacgactgc tagtttacat
    10081 ccagtacgat aagccggatc aaatcccagg acaactttac cttttagtgg cggttgcaac
    10141 aaaagattac ggaggttttc gccgaaaata gcgattgctt gctcatctgc cttttcagtc
    10201 aattcattgc gcagttctcg ctcgattgct ggctgaataa agcgtttata actatcctga
    10261 taggcttctc tgacgaatga agaagaagga gaggcaggat ttttgactat ttggcgatcc
    10321 aagtaagcta aaattgctgc ttcatctact tgaaagaata ctttcagaat atcttctttt
    10381 tctccacgat tagtagctaa tattcgatga gaaaccattt tatggacggg ttctgcaaag
    10441 tcataataca tttcatatac gcctttttca tctgcttgct catccttgac attgctgaca
    10501 tacatacctt tgttatacgt ataagaccga atccaagtcc gaaattttgc attatcgctg
    10561 acttgttcag caataatctc atgagcaccg tgcaacgctt cttcggcaga agagacttct
    10621 ttttctttgt caatatattt ttctgcttca gattgcacct ctccatctgt aagctgcatc
    10681 agccacttcg cgagtggttc aagtcctttt tctttagcaa tcgttgcttt tgtccgtcgt
    10741 ttttgtttgt atggacgata caaatcttcc acttgttgca ttttcacggc ttgggtgatt
    10801 tctgcttcta actcgggagt cagtttccct tgttcatcga ttagccgaat cacttcagtt
    10861 ttacgttttt ctaagttttc aagataggca taccgctctt cgatttcacg aatctggact
    10921 tcatcaagac ttccagtcat ttcttttcgg taacgcgcga taaaaggaac agtgtttcct
    10981 tcatttaaca aattaagtac agtagtcaac tgcttaggac gatagtctga aagttctttt
    11041 tgtacaagct gaatgatcgt ctggttcaaa ttttccgtca ttaaaatgcc tcttttctgc
    11101 taaaaattgc ttctttattc tatcatactt tttatcctgg gttaaactac tgaaagaaac
    11161 aatattctct ataaaaataa aaagagccgc aacaaagtca cagctccttc attctttcca
    11221 ttagtcagat tccatgccaa ggtccttcgt tgttctgata ttcaatatct tgaagaatac
    11281 cttccttgtc aaaaacaacc ccatgcaatg accctccgaa tacagcccca ccgtccattc
    11341 cgattttatg atcctgaatc cagaggtctg ttgtctgcat atctccatgg agcattggtg
    11401 taatcgtatg tccaaataca attgtcttac ctgtattgtt tttcccttca tgaaatggtt
    11461 cccttatcca aataaagtct cttagacttg tcttacgcca gtctttcttt gtcaaatcca
    11521 caccggcatg gacaaaaaga taattttccc attcataata caaggggcgc tggactaaaa
    11581 atttaatcag ttctgaatat ctcgattgga tcatcatcga gatttcagtt ggggagtatt
    11641 cctccgatgc ccctgggtga atcaggcttt caaacgtttc ttttccacca tttctcatat
    11701 aaggcgcata ccagtcctcc ggtgattgca gaaattgcaa aaaatattct tcatggtttc
    11761 cccggaggta aatggctcct gtttcttcga ctaattgctt tcctaataaa aaacattctt
    11821 ttgtacgagg acctcgatca ttgagatccc ctaataaaac aagctgatgg atctttggat
    11881 cataatagtg aataagtttt tcaaaaagct catgtttgcc atggacatcc ccaatagcat
    11941 aaatcaatga tttcatgtgg atcaccatcc tcaacgctag tatagcgcat attggaaatg
    12001 gtcgacaatc aaaactattg ttttctattt aattaaagct gtaaaccgca gttctgcttc
    12061 ttctggagag attccatggt cttgaacata gcgattgcgc ggatgtttcg cacactctat
    12121 gctacatgct cctaaatgtt tttcttcatt ttcaatggaa gcaagaatct gacgattgca
    12181 ctcaggattg ctacaattga tatatcgttc acatggtgtt ccgtcaaacc agtcgcgacc
    12241 gacgatttgt ttatctactt ggttgatatc gacagcgatc cgttcatcaa acacatacat
    12301 cttgccgtcc cataattctc cttggacttc aggatcctta ccataagtag caataccgcc
    12361 atgcagctgt ccgacatctt tgaatccttc acggacaagc caaccagaaa acttctcgca
    12421 acgaatgcca ccagtacagt aggtcactac ccgtttttcc ataaattctt ctttgtgctc
    12481 gcggatccag tctggaagct ctcgaaaact gcgtatatct gggcgtacag cccctcgaaa
    12541 atgtcccaag tcatattcat aatcattccg tgcatcaatc accactgtct gttcatctaa
    12601 aatagcttct ttaaattctt gcggtgaaag ataggcacct gtcacttggt ttggatcaat
    12661 gtcctcttct agctttaaac ttacaagctc tggacggtat cttacaaaca tcttcttaaa
    12721 ggcatgtcct tcgcttggat cgatcttgaa catcgtatca gcaaatcgag gatcagcttt
    12781 cattgctttc atataggcag tcgtgttttc aacggttcca gacacagtac catttattcc
    12841 ttcttcagct actagaatac gacctttaag atccaaagat ttgcaaaagg ctaaatgatt
    12901 tttggcaaat tcctctggtt cttcgatttt tgtataattg taataaagca gtacttgata
    12961 atccatgtat ttcttccttc cttcttttcg ttcctaaaca gtataaagaa ggcgttcgca
    13021 tttgacgatt tatttgcttg tgtataatta cgcatggaaa gttttttgtt tacataatat
    13081 tattatttga attaaaaaga cttcgctttc ctgtttcgca cagaaaaaca aagtctttat
    13141 ttctattatt tacctgtaac cgcaaagtag ttgccatcgg gatcagcaaa gttaaagaca
    13201 tattcttctc caagctgtac catatcacct aatgtgactg attgcgtctg cattttttta
    13261 tatagtccaa agatatcttt actgtaaaac atcaaagatg gggcactagt agctgtgcca
    13321 ggtgaatggg actcaacaaa tgctcgatca tataacagaa atgaggtttg actgtcctca
    13381 actcctactt caattataag cgtcccatcc atttcttgac gatctttttc cgcaaagcca
    13441 actgctctcc aaaacgttgc atttttttct acatcgtcca cataaagcat gatttttatt
    13501 tcattcgtaa acatctgttt cctcctaaac gatctgttct aacattctca actttgtcag
    13561 tttaccatag gaaaatggga aaatctacga aagctctcaa cagaatcgtc ctacttaaat
    13621 acgcttttct ttaataataa ataagttggt tgtttttgtt atttgcgctt tcacttttcg
    13681 gaataaataa tgtgtattta tatggaataa ataaaaaaag aggtcgtgac taaaaacaag
    13741 tcacaacctc tggtaaacgg tgttctcctg acaactcctc ggtcacaaga cacaatattc
    13801 aacaaacatg agaagctatt ttttgaaatt gctctttgtg actcagagtg aaacggcagg
    13861 ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagctcaacc
    13921 aaacgtaaaa tatgaggaac tatttttgtt tggctgttct tattacttga tgcagggcag
    13981 ctttttcaca acctcttatt cacggtgttt cagaagtagc ttctcggaaa taagccgaaa
    14041 tttcacaaaa atttgagaaa ctatttttag gaaacttctt cttatttctt aaagctgacc
    14101 acttctatca caacctcttt ttgactatgc attcacatcg atcactttag aatcatcatt
    14161 gatgacatcg cgatctaaat ctttgatgcg tgcacttgtc acgataccag ctaccatgct
    14221 accgctgacg tttactgctg tacgtgccat atcgattaat ggttcaacag agataactaa
    14281 cccaacgatt gctactggca agttcattgc ccctaaaacg atcagtgaag caaatgttgc
    14341 gcccccaccg actccagcta caccaaaaga actgattgtt acaattgcca caagcatcaa
    14401 aataaattcc aaactaaaga cattcactcc agctgctggt gcgacgatcg ttgccaacat
    14461 agctggataa acgccagcac agccgttttg tccaatagac aaaccaaaac ttgctgagaa
    14521 attagctgtt gcatcatcta ctcctaaagc ttttgtctga gtctcgatat tcaatggcaa
    14581 agctccagca cttgaacgtg aagtaaacgc aaagctcaat accggaaagg cttttttgaa
    14641 atagttgatt ggattgactt ttgcaccaat caaaatcagt gagtggacaa tcaaaacagc
    14701 aagcaatgct acgtaagaag caacgacaaa tttacctaaa ttgacgatag ctgcaaaatc
    14761 acttgttgcc aaaacgtttg tcatcaaagc aaatactcca taaggtgtca aacgcaaaac
    14821 aagcgtcacg atccgcataa tgattttata cagactgtcg actaaatctg aaaagaatgc
    14881 tgcttctttt ggcgcttttc ttttcacgcc tagataagcg attccgacaa atgccgcgaa
    14941 aataacgact ccaatcgtac ttgttgcacg actacctgct aaatcagcaa aaacgttttt
    15001 agggataaag gataaaatct gctgtggaat cgataagttt tcaactgttt cctgacgtgt
    15061 tgccagttca gagattcgtg ctgtttctgc agctccttgt gtgaatttag ctccttggag
    15121 tccgaacagc attgttgaac caattccgat caatgctgca atagctgttg ttccaagtaa
    15181 agaagtcaat acagtcgcac taattttccc tagcttttca gaaactttca tttttgtgaa
    15241 tgcaccaatg atcgaaataa acactaaagg cataactagc atttgcaaga atgatacata
    15301 tccttgacct acaatcccga tccattccat tgattgagta acgactttgt cgtctacacc
    15361 aaagatcaat tgaagcagac cgccaaagac gataccaact cctaatgctg taaagacgcg
    15421 tgttgaaaat tttgtatggc gcttttgcat acgatagaac acatataata atgccgcaaa
    15481 aactagaacc actaaaactg tgatgagtgt tgtcattttt ctcctcctca tattattaat
    15541 gacaagtcca tcgaaggaag taaaggttcc cgagaatgat tcttcaacca tcctcctgaa
    15601 aaaatttttt tcagtgactt ttatatttta gacgattttt atcgaaatga cattcttttt
    15661 gcttacaatt agtaataaaa aaacatacct gtgatagctt taatctatcg caggtatgtt
    15721 tttactgtct attcttgttt ttctgattga aaattcaatt ttaccccagg ctgatctaag
    15781 gcgatctctg tcacctcata agacattcgg cgtacgatat cgaataacgg tgctactagc
    15841 tcgtccactt cttcttgtgt gacatcattt tgaccttcaa ttttccgatt gaggatatga
    15901 ttgatctgac tcacacttcc agataaaaca tatgtatcaa acactaaacg gaactctaag
    15961 cgtgctccta cgatgctatg ttctttcgga tagttttcga tcgtttcttt taaaggcgag
    16021 aagcctactt tcaaatctgt ttctacttct tggttcggca atctcatatc ataatgaaat
    16081 gcttctacaa cttctttttg tcttctgatt tccatataat tttatcactc ctttatgaaa
    16141 attataacat atctcctttg aaaggggata ctacttttta atgatgctct gcaaggtaag
    16201 gatttcattc agatttccta ccacataatc tgccgggatc tcacccagaa aatgatcgat
    16261 gtcgtaaaga caagtagcga ctcctgcatt tttccctgct tcgatatcta aacgacgatc
    16321 gccgatcatc atcgtgtctg ttctttctaa atgaaaagta tcgatcaaat aatttaaaga
    16381 tgctggatca ggtttacgag gaaagtcctg atcgatccct actacttcct caatcagatg
    16441 tgccaatcga tgttctttta acaggcccca tgttgattct gtcaaacggt gggtcaggat
    16501 gaaatgacga ccgccgttgt cttttagcat ttccagtact tgtttcgttt ctggaaatgg
    16561 acgggaaact tccgtacttt ctttttcata tcggtgaaat aattcattaa actctctggc
    16621 attcaagttt gattcattga tcaaataact agtagaatac tttttcattt tgaagtagat
    16681 ttcttttttg tccatactga taccaaaatc ttttagtgct tgccatgcac catctaccat
    16741 tgcgggatac gtatcaaaca atgtaccatc aaagtcccat atataatttt tcatgttcct
    16801 tttgttccat tcctttgctt cttttttcta tctctcactt ttagataaag gtgttcttta
    16861 aagtattttt ttgtccagaa ttgtcaaatc atctgctaat gtatactgga ttggttgtgc
    16921 atttgggata tcgatcgtat ccatttgatc ttcaggaaca tcttctaaat attttgtcag
    16981 tgctcggatg ctattaccat gacccacaac tagtaaatct tgccgatctt ttagttttgg
    17041 tgctagatga tcttgccata agggaatgat ccgattcact gtcatttcca gactttctcc
    17101 gtatgggatc aaatgcggat ccaagtgatc atatctgcgg tcaaatctat tttctgcggt
    17161 taacggcgga acttccgtat atcctctgcg ccatgttttt acttgatctt ttccgtatat
    17221 ttctttcatc ttatctttgt ttttacccac aagatcccca taatgccgtt catttagacg
    17281 ccatgttttt attataggaa gccataactg acccatttct tctagtgcga cttgtccggt
    17341 aagaatcgca cgtttcaaaa cagaggtata aacggcatcg aattgaagtc cggcttcaag
    17401 cattttttga ccggcaactg ctgcttgttc ttttcctttt tctgttaagg cgacatctag
    17461 ccatcctgtc cagtaatttt caaaatttgc ttcgctttcg ccatgacgta ataatactaa
    17521 tttcattctt ttctccctta aacattcgtt tcgaatttta taccacatgt tttgcatgtc
    17581 acacgtattc gtccttttcc tttaggtgca cgctgctttt gatgacaatt cgggcactca
    17641 aaaaaagtat aactttttcg atccattttg acttgctttt tgttttttat ctgatgatat
    17701 ttttctttga ctctatcaag atatttcaaa taacgttgat tctcatttag tctggggtag
    17761 attttttttg ataggaaacg aaatatcatc catccgaaaa agacgatgaa caacaattgt
    17821 ccgattctat agggcaacca acgacttagc aaactaaaga caagcgcagc gatcaataga
    17881 ccgcgattca actgatcaaa tttcgcatat cttccctgca taagtttgtg atagttctgc
    17941 tgaaatcgta ttagcctttc taaccatgtt tgcatcatat tatctcctcc atcatctaat
    18001 atttattatg acaaaaaaat ccaaaaagaa aaagccttaa ttggcctttt ccattgcaac
    18061 ttggtgaagg acattccgat ttctggtaaa gtcaatcaca atcactttct ttttatcttc
    18121 tctgttgact actcgaaatg tgtccgtcaa tggaagaaac agcttcttag atggtagctg
    18181 ttgcatacgt ataaaacgat ggatcatcgt aatcaattga ggaaattgaa tagacgtgtg
    18241 aagcagacct ggatattttt tcaacagctg tgtcgttttg atcagattcc gttttttact
    18301 gatcactttt aaaaagcttt ttagaatctt ttgctgacgt ctaaaaatag aaaaagaatt
    18361 ttcgtcatct atttgataac gcatttgttt ttgcagttct gctgcagtca gatggatctt
    18421 cccatcgttt gtatcgatca ttaaaccttc tggaaacagt tcaaccactg ctcgatccca
    18481 agattcaaga gaaagctcga tataatcttt tactggtaat tccaattcta ccttaaaaaa
    18541 gtgcaaaaca gccgacagcc cttctgcttg atagatttct cctaatgtta gcccctctag
    18601 atggaactct ggtgaaaaat gcagcaatgt tttattttcc tgcaattgaa catatagaaa
    18661 tgtagatcgc tcaatcagag tatcttttaa aacaactaaa aaattcattt ttctccccct
    18721 tttttcatca tttttaaaat agcatatttt gaaaagaaag tctgatgctt agagtagaat
    18781 ttcataaaaa tttactattt cataagtatt cacgagaaaa atacaaacaa aaaagcggaa
    18841 ataacgctca tccgccttct tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt
    18901 cagacttttc tctatcgtta ttttcatcta aaagttcttg cattccgata atttctcgat
    18961 attgatgaat cttagtgata ccaatgactt tgtctacatc aatactaaaa caaataccat
    19021 tacctggaat atcgatgttg atgccttgac caatcgcatc cattacttta ttcgtcaaac
    19081 tatcgggtac taatgtcaac acaatatctt tttctggcga aatgtccaaa cctaaaaatt
    19141 tccctgaatc atgtacacca gaacctcttc catgtaagat cgttccgcct gctgcacctg
    19201 cttcttttga aaagtcgata acatcttcgc ccattcctcc gtcaacgatc gtcacaatca
    19261 tgtgaagatt caatgctaca agatctttca tttgctttcc cccaattttt gcaatctttc
    19321 tttttcgatt ctgtcttttt catcttttcg tttataaata tatcctagta ctaaagtgga
    19381 taatattggt gtcaatgcaa tcattgcaac cacaccaaac ccgtcaatga tcggatctct
    19441 tccttcaatt tgtgcagcga ttgccacgct cattgataaa ataaatgtcg aacacatcgg
    19501 gcccgttgct actccaccgt tatcaaaagc cattgctaaa aagatcttgt ccacttttcg
    19561 tcctaaaata aatgctagcc cgtatcctgg gatcaagaaa taataaagag agaatccgac
    19621 gagcaaacgc cacatagaca atcctactgc tgcgccgatc ccaattgaaa ttaccatcag
    19681 catgattttc gatcggattc tcccctcgct caattcttca acctgtttga tcattacatg
    19741 aatcgccggc tcagcaaacc ctactaagaa ccccattaaa aagccaaggg gaaccagtat
    19801 ataattgtaa ggcaatcccg caatagcact gcctaaatgc tgtcctaccg gtacataagc
    19861 catattgact ccgtgcagga aaaggatcaa cccaatagtc gttatcaaga aacctttcat
    19921 aatgtcaaag aaacgctttt tgcgcatacg aaaactaatg atattcatta aaacaaatag
    19981 gattacgata ggtgccactg ccaaaatgac ttccatggta acgatttcga ttccatctat
    20041 cacttgctgc cagaaactca ttggaagatc acccccaaaa tcatcacggc aagaatcggc
    20101 cctaacgaag caataccgac cattccaaaa ctgtcatttt catttttctt ttgtttgatc
    20161 atactagtca tccctccggc aagggccaag ataaatggaa cagttactgg accagtagtc
    20221 actccccccg aatcaaaagc aatcggcata aattcggaac tggtgaaaaa tgaggcgatc
    20281 aaaacgccta tgtagcctat agccatcaat tgataatagg atagcttgaa tacgacccga
    20341 agcagcgcaa aggctagaaa tactccagtg ccgacgctga cgatcccaat cagcaacact
    20401 ctgccaatct ctccgtcaga tatatcatat acttgttgtc ccaaaacttg cacggaaggt
    20461 tcagcaatcg tgatcacgat cccgatagca aatcccaaac tgaccaaaac ggtcaaactt
    20521 ttctttttga tcatatattt ccctactaga tctccgactt ccatcataga ataatctgct
    20581 ccaaacaaaa agagcgtcat tccgatcatc atgattgccg caccaaccag aaatgaagcg
    20641 aggtcttctc cttctaacgg tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc
    20701 ggcaatatag cagcaatcac ttctttaaaa ttttttagca tgtatcctcc tcctttactc
    20761 tttaagttta ccttaaaatg aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa
    20821 tttttctcaa cataaaataa cacacgtccc aaaagtatct ttttacttgg aaaaatgact
    20881 ttgaaggaac gcgcttattg tgttttttta ttttacagag ccgatcattc cttgaacgaa
    20941 ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa gtagcgatga ctaatccagc
    21001 catgatcatt ccatagtctg gtgtatagga agcacccata gcagataaaa caagcggaac
    21061 cgtccgttta tcaggagatt gcaaagctac taagggccat aaataattgt tccagctact
    21121 catgaatgtg atgattgcag ccgcggcata ggtattttta gccgtcggca tatacactct
    21181 aaaaaaaata cttagttctt ttagaccgtc taatcgagcg gcttctacta gatcttttgg
    21241 aaaagattta acattttgtc gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag
    21301 gatgacaacc aagaaactgt tgattcccaa aaccgttccg ttcaattgtg aaaacattcg
    21361 atataaaggg atcatcaatg ccgcaaaagg aaccatcatc gatagcagta gaatattgaa
    21421 aattaggtct tttcgtttac tttgataaat ctcaaatcca tatcctgcca ttgacgaaat
    21481 cagcaatgca aacacagtgg tcaccaaagc gatcatcgct gaattccaaa gggaacgagt
    21541 aaaattcagc tcattggaaa acaaattttg aaaattgatg atcaagttac tgccaatctt
    21601 tatttttccc gatgtgatat ccacgggtgt attcgtcatt cccaaaacca tccagacaaa
    21661 agggaaaatg gaaataaatg ataggatgct taatgctgta tactttaata ctgacccaat
    21721 tgtcgaacga agttctaatg cttgtttttt gacgttgggg cgttcgattg tttctttttc
    21781 taccatattt ttcatcgact ttctcctcct actttcgttt ggattatcga caatatcaca
    21841 ataaaggaaa caataacaaa ggaaactgct gccgcatatc caaaattagg cgtgaattta
    21901 taactcaaat tatagatgta ttgagatagg gtggtcgttg catttgctgg tcccccgcca
    21961 gtaatatttt gtacttcatc aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta
    22021 aacaaaatga ttggttttaa atttgggatc gtcactttga taaactgctg ccatttagaa
    22081 gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa tattttgcat gcctgaaagg
    22141 aaaaaaatca tattgtatcc agtccaacgc caagtgatcg aaagaatgat cagaactctc
    22201 gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac
    22261 aagttgttta caaggccaga ttgagaaaat agacttttca tgatcagaga ataactgacc
    22321 atcgaagtga tacatggaag aaagatagcg gttcgaaaca accctttata tttcaacttt
    22381 ttatcattta aaaggttgga aataaaaagt gctagaaaca acatgatcgg tacttgtaca
    22441 agtaaataca ggaatgtatt aaaaaaagct tttttaaaag ttgcatctgt caacatccgc
    22501 tgataattac ctaacccgtt aaatgtcata ttgacagggg gaccactttg aaaagacgtg
    22561 atcaaagcca aaaccatcgg aataaaaacc atgattgata tcaaaatgac tggcaagatt
    22621 aaaaaagcat gcccgttttt gaataaatct ttctgcttca ttcttttccc tccaattcta
    22681 tcattgatta tctacatgaa aagacaagaa agacaatatc tgtaaggcag acgtgatctt
    22741 ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt cactttttta ccaccgatca
    22801 attagccaat tgtgcttcta cttgtttttg tgtatctgct agtaccttgt ctgtctcttc
    22861 tccattgatg attcgatgaa gagcttcagc tacgactgct tcaattgcat aggtctcatg
    22921 tccgtaattc acttctggta tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg
    22981 accactgtaa aattcagaag cttcttgata agcttcttgt tcattcgcgc taagcatcgt
    23041 agatactagt ccgatttctt tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt
    23101 tgcttctagg aaatctttcg ctgaatcttc tccagctact cctttgatca cataccagcc
    23161 agcgccgcca aggttagaag cttgcgcttt ttgcagattt tctggaagtg caggtattgg
    23221 tgcaatcttc cattttccag attggttttc tgctccttgg atcgttgaag aataccaagc
    23281 gccagttggg ctagaagcta ctgctcctga ttgtatcgca gttactccag cattccagtc
    23341 agaggtttgt tcgactagat cttctttcaa aagagtggca aacaattcaa gaccatactt
    23401 taaagatgca ttgtcttgga tcgtgactgt ctccccatct tcagatgtat accattctcc
    23461 tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa ggattgactt cagcgatttt
    23521 ctttcccgtc ttttctttga catctcgcgc tacttgaata tactcgtccc acttcagatt
    23581 ctccatatcc tcttctgtat aacctgcttc tgccatcaga tccgtacgat agaagtttgc
    23641 tgttacgccc gaatcgaaag gaacaccgta tattttatct cctactttgt ttaccgcaaa
    23701 tttatacggt gcaaaatgat cttcctttac aatagcagac aaattagcga atgcatctgg
    23761 ataagaagtc aaatagcctt gtatcctata atcttcaatc aagacgatat tgggcaatcc
    23821 ttctgtattc ccgctcgcta aagcggtgtt cagtttctga acgatatcat cctgagacat
    23881 cgtaacaact tctatctctg tttcttcgtt ctcataaact ttttttgctt cattcactgc
    23941 tttgatattg aatgtctcat cccacgccca gacagtgact ttttcttctt tgtctgctgt
    24001 actactagca cttccgcatc ctgttaagaa acctgatgct gccgctaaac aaacgactcc
    24061 agcaattaac tttctgatgt tcatttttat ccgctccttt tccatccatt agttgtttac
    24121 gtttgctaca ttcatcatac caaaatttga aaacggtttc tttattattc agatacttca
    24181 aaaatttctc ctaaaataat aaaaaaatac aaatcaacta tgacaaaagg aataatctta
    24241 gcatctagac caaagatcga aatggtgagg tttctttaaa ataaaaatat ccttgcattt
    24301 tctcctagaa ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc
    24361 caatacgcgt aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga
    24421 tcaattggtc tgagcacggg tttcaattaa tagggacttt ttccagtccg ctgaaagcgc
    24481 ttgatttttc tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat
    24541 taaatggaat cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta
    24601 tcctctctag ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg
    24661 attatctttt aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt
    24721 cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct
    24781 gcgagaatct ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt
    24841 ttgaacacac cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg
    24901 ctctaaacaa aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat
    24961 ttatcctgtt tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga
    25021 tacacttagg aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt
    25081 tgtttctgat tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg
    25141 caccaaagga caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct
    25201 tccgttatta accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa
    25261 agactacgct ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc
    25321 tattgatcca gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc
    25381 tttggctgac gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc
    25441 tttattagcc gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga
    25501 gaaactcacc cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat
    25561 ctacgagttc gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa
    25621 atatcatttt tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt
    25681 ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg
    25741 gcttactttg acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag
    25801 agtttttaaa gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa
    25861 atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc
    25921 ggtctttttc tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc
    25981 tatatccagc tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt
    26041 gaaagtttga atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc
    26101 aaaagctatt tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca
    26161 atccaaaaaa tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg
    26221 atctttggag aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc
    26281 gattcttttt ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa
    26341 atgctttttg cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt
    26401 gtcaaaaaac tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca
    26461 tccaagcaac tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc
    26521 ttcatgaacg aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga
    26581 tcaatgattt tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg
    26641 acccgtcttc ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct
    26701 ttatctaacc aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct
    26761 tttgctggct cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg
    26821 attgattcgc taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga
    26881 agccatgaag tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat
    26941 cattatgaac agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg
    27001 caagcacaga tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt
    27061 tggcagaaaa aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga
    27121 agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa
    27181 agttacatcg atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt
    27241 cctgatgaac tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac
    27301 gcctatcttc atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca
    27361 aaaaatcagc ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat
    27421 accaaacaaa aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata
    27481 cgcctaatgt atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg
    27541 actgtaacga tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga
    27601 atagccaagc gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt
    27661 catctacgcg tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct
    27721 ccgagcatct tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac
    27781 aaatcgatca cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta
    27841 cttaagttga tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga
    27901 ttagcaggta gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc
    27961 tttcggtcac cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca
    28021 gctaagatct ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct
    28081 tctttcggat gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa
    28141 ggaacattag gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt
    28201 gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga
    28261 acgatttcac ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga
    28321 acagctcctt ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac
    28381 aaagcgcgca aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc
    28441 tgacaagccg cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc
    28501 ttttgatcct ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga
    28561 ttcgatgtgt cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt
    28621 cttctcttgt ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat
    28681 caactagaca gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac
    28741 gccgattttc tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg
    28801 acagctttct taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa
    28861 aatattcggc aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact
    28921 ctttttgtaa acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac
    28981 caatcagcaa aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca
    29041 aaaaatgccg gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca
    29101 gctatcaaag cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact
    29161 tttgcgccat gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc
    29221 tttgctccca ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact
    29281 tttaattctt ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct
    29341 agaatgggca attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc
    29401 aaatgactcg tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg
    29461 gtaatcgctg cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc
    29521 ccgacatttt ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa
    29581 gcagctgatg aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg
    29641 gtcactatga tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct
    29701 tccgtatggt tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat
    29761 gccgcaaaca gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata
    29821 ggtgataaag agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct
    29881 tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac
    29941 aacgaagcat ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat
    30001 aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag
    30061 tggctaccgc gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat
    30121 tgttggaagg catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc
    30181 gtcgttcctt cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct
    30241 ggtatcctaa cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc
    30301 tcctatttga tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac
    30361 aaatcctgtt ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct
    30421 tggatcttca aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca
    30481 ataacgccag gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct
    30541 tgcttatgga caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt
    30601 tccgattggt tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct
    30661 gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga
    30721 tctgaggaag cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc
    30781 ggatactctt gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga
    30841 aaatgactta gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt
    30901 gctttggttc cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa
    30961 ggtgtaaaga tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat
    31021 ttatgagaac cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat
    31081 gtcacgactt gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg
    31141 attctttcaa ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata
    31201 acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt
    31261 tctactgctt gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga
    31321 ttggtctgac gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa
    31381 acatgttgtg ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc
    31441 cttcactgca gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt
    31501 cgctaccaca gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact
    31561 atccgaacgt tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag
    31621 tggcttgtac aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt
    31681 ggtcattccg ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc
    31741 ttcccatagg atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac
    31801 cccaaattct acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc
    31861 tagcttcgtg tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac
    31921 ttctacaatt cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat
    31981 tctttgctca actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat
    32041 ttcttgaatc gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg
    32101 tcctgctacg atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac
    32161 acgcatggtc gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg
    32221 gtatttcata ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag
    32281 tgattggtct ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc
    32341 tttattttct accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga
    32401 cgtgatccga acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg
    32461 tctgcgagcg agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc
    32521 ttcaataatg gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt
    32581 acagtcctcc taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc
    32641 caatcttttc tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta
    32701 tcgcgtggaa taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt
    32761 gaagtatcct ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat
    32821 gagaagtgat ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc
    32881 ctgctgtatt ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc
    32941 cttggtcaaa gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga
    33001 ttggaacagc atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa
    33061 gcgttgcatc tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac
    33121 gtaccggcaa agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg
    33181 ttccgacaag gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat
    33241 ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg
    33301 aggccacgaa tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc
    33361 tgcgggtaaa atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt
    33421 tactggtctc acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt
    33481 ggaacatgat tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt
    33541 tttgaggtct ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa
    33601 tatgaatatc gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct
    33661 tttaaaaagc tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc
    33721 aacttctttc atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca
    33781 agcatttgca ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc
    33841 cgcacgagcc attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat
    33901 cacagggaaa tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg
    33961 tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc
    34021 gactgcaatg tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt
    34081 caatccaaca ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt
    34141 acgtgcgcca atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc
    34201 catcacttca gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa
    34261 tccttcttct tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc
    34321 tcgcagaatt tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa
    34381 tccttcgata gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc
    34441 gtctacatcc acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt
    34501 taaaatacgc acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact
    34561 tcgcgtatca ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt
    34621 gaatccttct ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt
    34681 tttcataatt actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg
    34741 attttctcta ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt
    34801 tgttctcatt ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg
    34861 tccctgtagg aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa
    34921 aaatataaaa tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa
    34981 tctttagcca tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa
    35041 cctatctaaa atagctaaag taatacgtat tcagttttgt aagttttcta gatttcataa
    35101 aaaatcgctc caaactgatg taagaattgt acctagtata gacataaaaa aatcgactgt
    35161 caatcatttt tatgaaactt tttttaattt tctgatagtt aactttgttt ttcagaaaaa
    35221 taacaattgg ttttattgaa gaaaatagtc atgaacaaaa gtatctgaga acttttttac
    35281 tctcttgttc atgacatata gaataaagca gctttgtctt gtaaactccg tttttcaaat
    35341 aataatctaa agattgctac tgaataataa agccgatttc atggtcctct tcgctgctga
    35401 ttcggtaagg tgactcaaca tccgctcccc agctattgat acccccgact cctcttactt
    35461 ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag ttcttctata tgtgttgcct
    35521 gttccagttc ttctgctttg aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt
    35581 caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt atccaacgtt tgatttcttg
    35641 ttagttctac ccatttcgtt tccatatgca tgccgcattc ttggggaaca aggtatgggg
    35701 aaacggggag tcctgccact ttataaatac cttcgacacc accagccatc cgatccggat
    35761 atgtttcccc tgataggcct tcatactcaa aaaattttgc tttcgttggc ataatgaacc
    35821 ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc ataataatgc atattgacaa
    35881 gcattttacc ctctccagcg atttggtagg aaattgtcac ttgggttttt ggtactgtgt
    35941 tgatttccag tgtaaaaatg atttcaaccg tttcagcgta ttcattactt gaatattgat
    36001 tatttactgg agcaatcggc aaatcgatct ttttattgtc caccagtact tctatttctt
    36061 tgacttttgg atacaatccg gctcctaacc acataccgga tttaaatcca aatccgctac
    36121 ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag tgtttcccgg tatagccatt
    36181 cttttccgtt tttattgagc gattccagtc cgcctcttgt ataagaaaaa atataatgcg
    36241 tattccccga tttgacacct aatgtacagt cgccgaagac gattgcaact ttttccgtat
    36301 ccatatgact tttatttact gtatttttca taataatatc ttacctcctg catggcgggt
    36361 ttttcttgtc catctgcaaa aacgatccca ttccctgaga attcatagtc acttggttta
    36421 tcgtcaaagt caccgccata gcgcatcact tctctgcctg tgattggatc agtcacttgg
    36481 atcgcctgat ctttaaaatc ccatatatag cctccttggt acatttcata acgatcaagc
    36541 aacgtcatgt aagaatccat tccacctaac gaattcccca tgtcatgcat atattcacat
    36601 aagatgaacg gtttttccgc tttattttca aggtagtcaa tgatctcttc tggtggcgca
    36661 tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct
    36721 tcataatgga caagtcgatc ggggtctact tttttgaagt aatcgttcat ttgtcgaatg
    36781 ttttcttccg cataagattc atttccaaga gaccaaaaaa gaatacttgg atggtttttt
    36841 aatgtttcga aattcgtttt tgctcgatca agaacagccg cctgccactg aggtactgca
    36901 cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt gccatgagcc atgggattcg
    36961 aggttcgttt ccgccatcac atggattcct gcttggtcgc ataaataata ccaagtgatc
    37021 tggttggggt aatgagatgt ccgtacgccg ttgatctttg cattttttat gatagccaaa
    37081 tctttttcca tatcttctat agtgatggcg cgtccttttt tcggatgcca ctcatgtcga
    37141 tttaccccgt tgataatcat ccgttttcca ttgaagtaga tgattttatc tttgatttcg
    37201 attttacgaa aaccgaagga atagtcaata tactccgtta ctcttttttc ttcgtctatc
    37261 gtctccaatt caagcttata taaattgggc tgaaaatgcc cccacagtgc cacattttca
    37321 aatccatcga tgcgaatcgt catttgctct tttaacggat attgctgttg atagatttcc
    37381 ttcccatcag gatctagtat cgctacattc agctgactgt ttttggctac ttgctcaaaa
    37441 gacaaatcta cttcaagttt gccattttga catttgtcat ctaagcgagg tttgatccaa
    37501 agatcttcta aatggttttg tcctcgagca actaagaaga catctctgaa gatcccaaaa
    37561 aatcggaaaa agtcctgatc ttccaagaat gcagctgtac ttcttttgta aacctccaca
    37621 gctagaatat tttcttcttc caaataaggt gttaaatcaa attcagacgg agtgaacgaa
    37681 tcttcggcat acccgataaa atgaccattc aaccaaataa acattgcttg ttctacacca
    37741 gcaaaatgaa tcgagatttg ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa
    37801 tacgaaccaa ccggattgtc tttagcagct gaaaagatcc cttccgcctc tttctcaggg
    37861 cttgtcgtga aagggggacg gcgaaataca tgtccctccc acggatacat cgtattgatg
    37921 taatggatct tgtcataatt tgctaattca atatgactag gaacagtgat ttcatcaaaa
    37981 gaacaggcat caaaatcttt tttataaaat gtttttggtc gtaccgaagg atttggcgaa
    38041 taacaaaaac gccatgatcc atttaaagac tgtttaaggg aggatgtggt ttctacttct
    38101 tcccttgttg cataacaaac atgatcgcta tgtgcatttg attggttcac tctaaaaact
    38161 tgtggatcat ctaaccattt tatatttgct ttcatacttt tttccttctc tctcataact
    38221 ttttgaaatg aagcagttct gtttggaaat caccttgaaa atcggggaag taaagtccaa
    38281 ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct
    38341 catcatctaa ccccatcaga tatacttcat ggaacgctgg ttgagctgaa gctagtatcc
    38401 tccctaaaaa cacaatagct tcatcttgtt taggagacac aaacatccat gcggcaatat
    38461 tttcttcaaa aggactcgcc aaacgataat aatggccata ctggatcacc ggacgaatcg
    38521 tttgatagga tataatttgg tcttttactt gcttttcttc ttcaggagac agctttgtca
    38581 aatccagttc ataaccaaaa acagcgctca tagctgtttc tcctcttgtt ttcaacgaag
    38641 tagatctgtc ggtctgatga tttggtacag ctgatacatg agctgtgatg gacgaaatcg
    38701 gataagcaag gcttgttcca tattggattt tcatacgctc cactgcatcc gtgttatcac
    38761 ttgtccatga ctgcggcata taataaataa acccggcatc aaaccgtcca cctccaccag
    38821 aacagccttc ccataagatt tccggataat cagttgtcaa atgttcaagt agttcataaa
    38881 gcccgagcat ataacgatgc gctacttctc cttgtctctg tgggtcaagg gtaatcgaat
    38941 agacatctga aagcgaacgg ttcatatccc atttgatgta gtcaagagga atcgtatcta
    39001 aaatctttct catttgtttc tcaatcgtct gccgaacatc taaccttgta aaatccagta
    39061 catgttgtga tctggaagca gaaggcattc tacctggctc ctgcataaga aaatcaggat
    39121 gtgttcgata cagttcagaa tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt
    39181 tcaatccttt gctatgagca taatctgcta tttcacgtaa tccatttgtc aacttgcctt
    39241 catactcgaa ccagtctcct aatgaagaat tgtccgcatc tctttttcca aaccagccat
    39301 catctaatac gaacatttcg atgcctagct cctttgcttc atcaatgatc gccttgattt
    39361 tttcactatt gaagtcaaag taagtggctt cccagttatt gaccagaatc ggccgttcag
    39421 caaattgatg acgacctctt gcgattcttt cacgtagcaa ctcatggtgg atctgactca
    39481 ttttgttcaa tccctgactt gagtaagaaa ggatcatttc aggtgtttga aaagactggc
    39541 ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc accgacgact ctcaattgat
    39601 tgatctgatc tttttcaagt tcgaaagaat ggttgccgga ataaacaaat tgaagtccga
    39661 tcgcttctcc agtattttct gtcgtatgat gatgaactaa agcaataaag ttattcatat
    39721 ggtgactgct cgttcctcga cggctttcaa atacttttgt tccagataaa acagattgcc
    39781 gtgaaatctg tctttcattg acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc
    39841 tagtatgggc aaagtctagc tggaaactag ccgctttttc tagtttgatt gtttctccgg
    39901 tttcatttct tatctttaca gatcgtgtga tcactgctct atcagtaaat atggtataga
    39961 acaaatcgaa ataaatcgct gtttctctat ccttaagtga aatcaccaac gtttgtgcct
    40021 ctgacttgtc catcacataa gtttgaggca agccttcgat ctctggcttc ccatctgtaa
    40081 tataatggct gtcatatctc agatccagta agtctgatcc attcttgcgc tgaatgatcg
    40141 atgcgggcgt tcgaaagtcc atgctgccat gagaagagta ttcttgcggt agtgtatctt
    40201 tcgacagaga ccggtcagga tttaagggaa catttcctga aaatccacga tctcttcggg
    40261 gataattttt attgtctttg tactgcttga ctggtttgcc aaaataaata tgcgccaata
    40321 cattgctttc ttctatcttt atcagataag aaagaaatgt atttcgtaaa tggaagactc
    40381 ggctcttttc atcaaataag atgaaatttt tatctgtttt tctatgcgta atatccatca
    40441 tttcactccc aacaaaacgg aaaccgttta ctttttttta taattcctgt atactgttta
    40501 ctaaacaatt ttgtcaataa aaatttttta tttttttgag gagtgtgtaa aaatggttgg
    40561 aattagagat gtcgcacgaa aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa
    40621 taacgatcag acgatccatg tggctcagac tactcggcaa cggatagtag aggcagctgc
    40681 tgcgttggat tatgacatca gtactcgtaa atatatcaaa aagagaatgc cttcgattgg
    40741 tgtgatctct acgatcggca aagcgagcga agaacaggat atctattaca aacatttgcg
    40801 agtagggatg gaagaagaag ctcgacgtct gcatttaggg atgaatcgaa tctataatct
    40861 atctgataat cctaaacaat gggaagatct agatcagtta ggtgcggtga tcgttgttgg
    40921 gactgttacg aaagaatcga ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt
    40981 ggataaccct gacatccagc aagaggtcga tatggtgtat gcagatcttg aacggatgac
    41041 taaaaccgta ctggaattgt tcttagaaaa tggtcataaa agaatagcct atatcggggg
    41101 atataaagtc gatatggatg aattcggtgg gaagcagatt tctataaatg aaaagaggat
    41161 gcgtgcctat cagcatttta tgatcgatca tcacttagaa aaatatatgt cttatcatct
    41221 aggtggctgg acagaaaaac aaggtgaggt acttgcgact aatctattag aaaatgaatc
    41281 ccctcttcct actgccattc ttgtcgggag cgatccaatg gcaattggtg tctatcgggc
    41341 attgaaacaa aaaaatatcc aaattggaga agagattgtt gtagcaagct ttgataatat
    41401 cgagacaatc gcaaacctct cccctggctt gaccagtgtt caaatcaatg cgaaatcaat
    41461 tgggcaagca gctgttcgaa tggcattgga gcgaattgac ggtatacggg acgaaccaat
    41521 cattttgact tatcctgcac gattgattgt tagggaaagt tttatcccaa gaacaaaaag
    41581 gtattgaaag agaggttgtg aaaagccgct tcgtttcttc atactcaaaa cgaaacggct
    41641 ttttcacaac ctcttactta tacttattca caaaatccaa tgtctgctgg taacaatttt
    41701 gcgattcttt tagcgtgtaa tcaaattggt attcatgggt aatctctttt tttgtatctg
    41761 tataaaacaa ggattgcacg ggtaccttta attctgtcaa ccgattttct aatgcttgac
    41821 cttgctcctg gaatgagtat gcatttccgt cagttatata cgttggcgga aattccttag
    41881 aaacatggtc agccacacta gcttctttca attgctcact cgttttccaa tcttttgttc
    41941 caatcaacga ccacgcaacg gttttgacga aaaacttcat aaatcgatca tccgaatgct
    42001 gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga aatggttcct ttcagactat
    42061 cttttggaag tagctgtttg attttcattt gtttagcata cgcatcattc gtttgtatag
    42121 ctgcatattg caatgcgatt tgtgaacctg cactatctcc accaaaaaat aattgttcca
    42181 tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc tttgatcagt tcattcactt
    42241 gaagaacttg attagggtat tctgaatcag gcgctaattc ataattcatc gcaaccactg
    42301 cgatgttggc gtctgaaact aattttgtcg caaattcttt gacacccgat ttatctccgc
    42361 cgacgaatcc tccaccatgt acccaaaata gaacaggaac aggccccttg ctgttttttg
    42421 gatagtagat gtcataagta ttctctttat acttggactc atacgtcaaa tctgtttttt
    42481 tcgccacgtt tttttctgct tgatcgaacg tttttttatc agtgatttga acagtatttg
    42541 aaaacatatg gttgatcaag aatgctccag gtctggggga aagctggaag gcaagaaaga
    42601 ttgcaaacgc gatcccaaga atgattccga agaaccatcc gataaattta agaattttct
    42661 ttttcataag gctccctcag tagttagtat taagcattct gattatacca tagtgttatt
    42721 tcctatcaag ctctcctaga tgatttaatg attttcccca aacttcttct gcaatgcttt
    42781 tgacaaaaga aattttttcc attgttcttc aaacaaaaca ttttcttctt ctgttgaagc
    42841 aaatccacat tgaggactta agcacaaacg atccaaaggc agatatcgtg tcgcttcgta
    42901 gatccggtca atcacttgct ggtgctcttc catttcgcca tttttcgtta cagtcaaaat
    42961 ctaagacaac taatttatct tcagagactt tctctaaaag aacaaaactc ccagcacgtt
    43021 cactatcgta ttctaaataa taggcacgga cattttcttg agtaaaataa tgggtctgct
    43081 acgtcgtcat cccccctgat gccgccaaaa tcgaataata atttccgcgt caaacatgta
    43141 tatttagtgt tgaatcattt gagaatatga aaataaaaat ctttttgatc aatcagttaa
    43201 tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt cattctgacc cagatttgat
    43261 tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa taatgtagac taactaaaaa
    43321 gactaagaaa caataacaaa agttaacact tcttagtctt tctttagcac gtctatttat
    43381 taaagatttt agggcctttt ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt
    43441 tggatcggcc tccaccttgt ttctttttga tcaattcttt cattttttct ttttggtcca
    43501 tttttattct cctttaacga atgaagcgac taattcgata tgatgggttt gcgggaaaag
    43561 atctacaggc tgaactttct tcacggcata acctgcttct gcaaataact tagcatctct
    43621 tgcaaaagtt gccggattac atgagatata gacgattcgt tctggtgaca tttcgattgc
    43681 tgattcaata aaatgatgat ctaatccttt tcgtggaggg tcaactacga tcacagatgg
    43741 tttgatccct ttttcttgcc attttttcat aactttttct gcttttccga cttcataatg
    43801 aacattttcg attttattat ttaaagcatt gaactgtgca tcttcaattg cttctggaat
    43861 aacttccatc ccatacacct tatccactct gtctgctaaa gacaagccaa tcgtacctat
    43921 tcctgaataa gcgtcgatca caatgtcttt tttatctaac tcggcaaact cgatggctgt
    43981 ttggtacaga tactctgctt gttgagtatt tacttgataa aatgactttg ctgagattcg
    44041 ataggttttt cctaatagct gatcttcgat atagtctctg ccatataaaa ctttttcttt
    44101 gtctcctaaa atcacatttg ttttttcttc attgatattt tggatgaccg aaaccacttc
    44161 tggcaattct tcatgaatag cttcagcaat ttccttggat ttaaagaatt tttctttacg
    44221 agtaactaaa acgaccatca tttcatggga ataatgacca cgacggataa tgatgtgacg
    44281 aagctgtcct tcattttttt cttcattata tcctcgtact tggaaccgtt gcaagatatc
    44341 tctgactact ttgattgctt gatcgatgac aggatcttgg atataaaaat cttcgatagg
    44401 gactagatca tggctattct ttttgtaaaa accggtggct aactgtccat cgatacggcg
    44461 aacagggatc tgtgctttat tacggtagcc gaatggttct gccattccga ccgttggaag
    44521 tacaggaact tctggcatct tagcaatctt cgccattaca tgctctactt gtttttgttt
    44581 gaagacaagc tgttgttcat acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa
    44641 tgcattgttt tctacaggct gacgatcagg tgattgtgta ataaatgttt cgacttttcc
    44701 atatccaaac ttattgcctg cttttactac tagtatttct acttcttctc cagggagtgc
    44761 attttcgata aataatggat aaccatcgat ttttgctacc cccattcctt cataactcaa
    44821 gtcgacgatt tcgactgtat atcgttcgtt tttctttact atttgctgtt ctgtcatatt
    44881 ttgctccttt aagcctattt ttcaatccta ctgaactcta ctataaatca gctaattaca
    44941 caactaaaac cggcactgac cttactacgt tcagcaccgg cttttgatta attcttttga
    45001 ttaggtgttt cgtctgctat ttttccgtca ccgtcgatat cttcatccgt taaacgttcg
    45061 acttctttta cgaattcttt gctgacaacc tctaattctt cttcatctgt acctgtgatt
    45121 gcttcatcag gaatctgatc ggtattggca aagaactcaa tgtgttgatg aagattttgg
    45181 aaatgcatcg gtgcttcccc gccgtattca ccatctaagt tgatcattac tggtcgattg
    45241 atatcatctt ctgtttcaac gaacaaactg cttgttttgg tatagattat tcttggatca
    45301 tcgacatgtc gaccgccatt cagcatgagt gctgcaatat gcaagatctc aaagatattt
    45361 gctgtcttca caatgattaa agaaaacttc ccatcatcca atttggcatc tggcgcgatc
    45421 tgttcgaatc ctccaacaga gttcgtcagc cctaggaaaa acatcgaagc atttccgata
    45481 tattcccctt catcatactt caagcgcatt ttgattggct ttactcgagg gagcatctct
    45541 gctcctttcg caagataagc aagataacca aaaatactct ttagttctga tggaacttca
    45601 taagtcaatt ctgtcaaata gccacctgca gcgatattga tgaagtaact gttatcagaa
    45661 aggccgatgt ccatttttac ggtttggtgt ttttttatca cttctgctgc tgctttaata
    45721 ttatctctag gaatttttaa tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg
    45781 gccattttag gacggtgttc aagcggagcg attccattaa ccacttcgtt gattgtgcca
    45841 tcgccaccag ctgccactac tagatcaaaa cctagttctg cgactctaca tgcttcattc
    45901 ttggctgaat ctggttctgg tgtagttgca taggcgctgg cttcaaatcc tgcttcttct
    45961 aatgcctgta ggatatccgc caaatttttt ttcaataatt cttttcccga ggtggggtta
    46021 taaattacgc gtgctttttt cattttcagc ctctatcgtt tcgctaattc atctttaagt
    46081 agcttattca cgacaccagg atttgcttgt cctttagttg ctttcatgat ttgaccgact
    46141 aggaagccaa ccgcacgatc ttttccattt ttgaaatcat cgacagattg ttggttgtta
    46201 tccaaaattt cattaatgat tggcagtagt ttgttcggat cagataattg cacccaacca
    46261 tttttttcaa ctacttcttg ggcttctccg ccatttgtga tcaattcacg gaagactttt
    46321 ttggcgattt ttgagctgat cgttccatcg gcgatcaaac gaatcattcc tgctaagttc
    46381 tcaggtgtca attttgtctc agctaattcc agtttctcac tgttcaagta agcagaaact
    46441 tcacccatca accagttaga tgcttgcttg gcatctgcac cattttccaa aacagcttca
    46501 aaaaaatcag acatttcttt tgtaagtgtc aataccattg cgtcatactc tggaagtcca
    46561 aactcacgga tatagcgttc gcgacgagaa gctggcatct caggcaaact agcttttact
    46621 ttttgaatcc attcgtcatc gatcacgaat ttcgggatat caggctctgg gaagtaacga
    46681 taatcactag acccttcttt gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa
    46741 cggcgtgttt cttgttggat ttctcctcct gaaagaagaa cttttgcttg acgtttttct
    46801 tcaaaagcta aacctttttt cacgaaagat aaagagttca agtttttcaa ttctgctttc
    46861 gtaccgaact cttcttgtcc ataagggcga agagaaatat tggcatcaca gcgcatagag
    46921 ccttcttcca ttttcacatc gctgacttct gtaaattgga taattgatcg caatgcttct
    46981 agataagcgt atgcttcttc tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa
    47041 ggcgtgcctt gtcggttcaa atcaacgtaa gaataaccgc caataccatg catgtttttt
    47101 ccagcatctt cttctaaatg gacacgttcg atccggattt ttttcttttt accttctact
    47161 tcgatttcga tccatccgtc atgaccgatt ggttgatcaa attgggaaat ttgatatgct
    47221 tttggattat ctgggtaaaa gtaatttttc cggtcaaaat gagtatcttt agagatttca
    47281 cagtttaatg ccaaagctgc tttcatcccg aattccagcg cagctttatt cattactggc
    47341 agcacgcctg gataacccca gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa
    47401 tgagctggcg ctggagagaa aatttttgag ttggttttta actcgacgtg gacttctagt
    47461 ccaatgactg tttcaaaatt cattcgtctt tccctcctaa aatagctggt tgttgtttat
    47521 ggaattctgt tgcttgttca aacgcatatg ccgcttgata catcgttttt tcatcaaaat
    47581 aattaccgat gatctgcaat ccaactggta atccttctga taaacctgcc ggtacagaca
    47641 tacctggaag tccagccaag ttgacaggga tcgttaagat gtcactcatg tacattgtga
    47701 ttggatcgtt aatgttctca ccgattccga atgcaactgt tggtgaagat ggcccgatga
    47761 tcaagtcata atctgcaaag actttttcaa aatctcgttt gatcaatgta cggacttgtc
    47821 ctgctttttt gaagtaagcg tcataataac ccgcgcttaa ggagaatgta cctaacatga
    47881 tacggcgttt tacttcttca ccgaatcctt ctgaacgtga gttgacatat acatcatcta
    47941 atgatttcac attttcagag cgataaccgt agcggatacc atcgaatcgt tgcaaattgg
    48001 aacttgcttc tgatgaggca atgatataat aaacagccac accgtatttt gaatgaggca
    48061 agctgacttc ttccacaaca gcacctaatg aacggaatgt ttctgctgct ttcaatacag
    48121 cttcttttac gccaggggcc acaccttcgc ctaagtattc tttaggtaaa gcaatcttca
    48181 ttcctttgat gtcgcctgtc agtccctctg caaaatcagg aactgaaacg cctgaagaag
    48241 taccgtcttt ttcgtcataa ccgctgatag ctgtcaatgc taatgcatta tctttcaccg
    48301 tacgggtcat tggtccgatt tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg
    48361 agacacggcc ataagtaggt ttcatcccta cgatgccgtt aaaagctgct ggttggcgga
    48421 tacttccccc agtatcacta cctaaagaaa ctggaatttg tccagctgca actgctgctg
    48481 cagaaccacc agaagagcca ccaggtactt ttgttgggtc ccaagcattt tttgttttct
    48541 tgaaataaga agtttctgta cttccaccca tagcaaattc atccatgttt agcttaccaa
    48601 cagggatcat atctgcttga tagattttat ccatgaccgt tgcatcataa attggttcaa
    48661 aattgtaaag gatcttagat gcagcggttg tcaagatatc ttttgttacg atattatcct
    48721 tgataccaat tggaatacca gcaaggacgt tgctttcatc aatccctttt tcatctaaag
    48781 cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct
    48841 ctgtttcttt gattcgttca aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg
    48901 atacaagcat atgatgcagt tcttctagag aacgattgtg taattctgtc attatgcgcc
    48961 agcctcccca ttatcgatga ttgcgggtac tttgataaag ccgtcttctg tttccggcac
    49021 atttttcaat aattcatcgc ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt
    49081 tactgtttca accacattcg acgtaaatgg aacaccttcc gtatctactt cttccagcat
    49141 ttcaaccata tcgatgattt tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc
    49201 gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt tcttcactaa tcgccatact
    49261 accccttctt tctaaacgag ttaaaaactt tgctctctat tttaacacaa gagtgccgtg
    49321 tctgaaattg tttattgaaa tttgtctgaa aaaactcatt tttcattctt ctactgagcg
    49381 acttttagcc aatgcatctg ccatttgatc ttctgtccaa acttcgattc ctaattcttg
    49441 tgctttggtc agcttgctgc cagcatcttc tcctgctacc acgatatccg tttttttcga
    49501 tacgctacca gtcactttcc cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt
    49561 aaatcgtgtt agtttaccag taaggactac tgttttttct ttaaaaggag actcgacttc
    49621 ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg acgcctgctt gttttaattc
    49681 attcatcaat tcatggactt cttcgttact gaaataagtg actacactgt cggcaatgat
    49741 atcaccgatc gtatcaagcg caataatcga ctcatagtca cttttactca atgtttctag
    49801 atcaccgaaa tgttctgcta aaatttttgc tgctttcgct ccgacatgtc ggattcctaa
    49861 cccgaaaatc aaccgctcga ccgaattatc tttgctgttg tcgattgctg tcaaaatatt
    49921 atttgctgat ttttctttta ttttgtccaa cgtcaataac tcttcttctg tcaatttgta
    49981 aagatcagca acatctgcta ctagtttttt atcatacatc tgttctagta cgcgcggccc
    50041 caacccatcg atattcatcg catttcttga aacaaagtgg ttcaaacctt ctttcatttg
    50101 agcagggcat ttaggattga tgcaccgtaa ggctacttct tcatccaaat ggacaagttc
    50161 acttccacaa actggacaat gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt
    50221 atctaacacg acttgcgaaa cttctgggat gatgtctccc gctttataaa tcagaactgt
    50281 atctttcaag cggatatcct tcaacttaat ataatcaccg ttatgcaggc tggcacggct
    50341 tactgtcgtt cctgctactc ggactggtgt catgatagct gttggcgtta ctacccctgt
    50401 tcgaccgact gtccattcga tgttttcgat tagcgtttca acttcttctg gcggaaactt
    50461 gtaagctgct gcccatctcg gtgctttgat agtaaaacca agttgatctt gtagcgagaa
    50521 ttcattcact ttgatgacga ttccatcgat ttcatagggc aaatccacac gtttgtcatg
    50581 atattcttca atgtatgacc acacttcatc aatcgaatga catagacgtt tttcccgatt
    50641 cgtgtggaat ccaatttttt ctagctcttc tagcgcatca tactgggttt ttgcttgcat
    50701 aggaccgaaa tcagctactg tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt
    50761 agaatctaac tgacgcaaag aaccagctgc ggcgttccga ggattcgcaa agatatcttt
    50821 tccctctgct tctctgtctt ggttcaattg gacaaatgac cgttttggca tgaaacattc
    50881 gcctcttact tcgatgttca ttggttcttt taatttgatt gggaccgacc ggacagtttt
    50941 taggttttca gtgatgtttt ctcctacagt tccatccccg cgagtcgcac ctctcacgaa
    51001 atttccatct tcataacgca aagaaacgga aagtccatct atttttagtt cacagcaata
    51061 gtcaactaca cgtcccactg ctttttgtac acgttgatca aaagctatca gctcttcttt
    51121 gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga gtgacttttt caaaaccttc
    51181 taatactttt ccgcctactc gttgagtggg agaatcagac gtgatcaaat caggatattg
    51241 ttcctcgatt gctactaatt ctgcgtattc tttatcataa acatagtctt ccactgttgg
    51301 tttatcttct acgtagtatt cgcgagacca ttggttcagt cttgtacgaa gttcttctgc
    51361 tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc atttaccctc cactactaag
    51421 ctttttcaat tggcgcaaag gcagccaaaa gacgttttac tccttgactt gggaaagcca
    51481 catctagttc tagatccttc gacgtcccgc ttactcggac gactgttcct agaccccatt
    51541 ttttgtgttt gactttatct ccaggtttcc agctgttatt ttcccctcca gtagcacttt
    51601 tagagggaac aaccgctgtc tctggctgtt tatagctata tgctgttttt ttagcgaata
    51661 caggcgtttt tttaggcgta ggacgcatgc cttcgatttc cagtaactcc tgatcgattt
    51721 cttctacaaa acgagaaggt cggttatatt gcgtccgtcc atataatgtt ctggagaaag
    51781 catttgttaa atacaacgct tcttctgctc gcgtgatccc cacatatgcg agacggcgtt
    51841 cttcctccag ttcgctttct tccatcaatg ctcgagataa aggaaaaacc ccctcttcta
    51901 gaccaatcag gaatacaact ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg
    51961 tcacttggga agcatcttct tctaaattgt caatgtcgga aacaagtgcc agatcattta
    52021 agaagaccgt tagtttttct tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt
    52081 gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa gttttcgatg cgtgcttgtg
    52141 cttctaatgt attttggatt tttaagtctt ctagatatcc gctacgatcc aaaacctctt
    52201 tcgtcaattc tgtcactgtc aagtatggga tcatctgagt gacttcttgg atcatctctc
    52261 caaatgcacc taattgctgt cctgcttttc caccgatatt tgccagatcc acattttgcg
    52321 ctgcttcaag cagtggccat tcatgaagag aagcaaaact gcgtagtttt tcgatagatc
    52381 ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc aaaactgatt gaatcttgag
    52441 gatttaccag cacattcaaa tacgctaaaa tatctttgat ttctttacga tcgtagaact
    52501 tgtgccctcc aaccatttta taaggaatat ttgctttcaa cagagtttct tccattacac
    52561 gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc gtaattccga tgattgcttc
    52621 gaatctcttc ttgcatccgg tctacgataa aacgggtttc gtctcgttca ttatctgcac
    52681 ggtagtaagt gatcttgttt ccttctttgt tttctgtcca caagtttttt ggttttcgat
    52741 tgctattgtt ttcaatgact tggtttgctg cgctaaggat gtttttcgtg gagcgatagt
    52801 tttgttctag caaaattact gcagcatctg gataatcttt ttcaaaatcc aagatgttct
    52861 gcatatctgc accgcgccag ccgtaaatac tttgatctgc atctcccacg acgcacaggt
    52921 tcctaaatct acctgccaat aaattgacta gcgtatactg tgcgtgattt gtatcttggt
    52981 actcatctac atgaatataa tggaatttgt tttgataata cgtgagtgaa tcaggatgtt
    53041 cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa gtccatacat tgattatatt
    53101 gaagctcttt ttggtaagca gcataacatt ttgccgcaat ctcttcgtaa taactgcctt
    53161 gcatttttgc aaaatcctga ggggtctgca ggctgttttt tgcttgagag attgttccta
    53221 atatacttcg aggatcatat tttttcggat caatgttcag ttcttttagt atccgtttca
    53281 tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt ccggttatac ccgataaaat
    53341 caacatctct tcttaaaata cgcacacaca tcgagtgaaa agtcgatacc cagacatctt
    53401 cgcctccaga tgcgagaatc gcattgacac gttctttcat ttcttttgca gccttattgg
    53461 taaaagtaat cgctaggatg ttccaaggat tgacttcttt ttcctcaatc aaataggcaa
    53521 tgcggtgtgt cagcactctc gtctttccac ttcctgcacc tgccattagc agtaatggtc
    53581 cgtcagtgtg aagaacagct tctttctgac gggggttcat cccgtttaat aattcagctt
    53641 tggatgacat taacaacatc ctttctttta tcaatcttgt taattatagc attttcttgg
    53701 ctttcattaa actagctagt tcaaaatcat ttgtttcttt ttatggattt ttccttgtca
    53761 attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa
    53821 aaatcaagaa attagataag actcagatat attttattct aaaaggaata tttgttggac
    53881 tgctgacagg aatcgtcgtt agcctgtttc gattatctat cgaaaagcta agcgatttag
    53941 ttcgaagtat ctatgaaatg agtcgggaac agccgattta cttaattggc ctagctattt
    54001 gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg
    54061 gaagcgggat tccacaagta gtgggacagc ttcgcgggga attatcaatg aactggttct
    54121 ctgttttatg gaaaaagttc attggcggtg tactttcagt tggtgcgggc ctttttctag
    54181 gtagagaagg accttctata cagcttggcg catccattgg acaaggagct ggacaattgt
    54241 ttcggagtcc atcttcagaa gaaaagattt taatttctag cggtgcaagt agcggacttg
    54301 cagcagcatt caatgctcct atcgcaggat tactctttgt tttagaagaa atccatcact
    54361 ccttttctcc acttgtttgg ctgacatcat ttgcttcagc aatcactgct aacttcgtct
    54421 ctctttactt tttcggcttg agacctgttt tatatctagg tgaccttcct tctttgccgt
    54481 tgaaatatta tggttcctta gtgatcttag gagtacttct aggcatctta ggttttattt
    54541 atcagaatgt cttgctggct ttgccaaaat tttacaaccg attgccttta ccagcttatt
    54601 ttcacgggtt cgttcctttc ttgctgattt tgccaattgg ttatttatgg cctagtactc
    54661 taggcggagg caatcaaatc gttttagctt ttggacatgc ttccttgcca atcattatga
    54721 ttatcggttt gtttgttctt cgattcgttt tttccatgat ttcttacggt gggggtcttc
    54781 caggggggat tttccttccg atattgacat tgggggcttt aatcggtagc ttttatggaa
    54841 acattatggt tgatttcggg atggatccaa tctacatcaa ggattttgtc gttattgcca
    54901 tggctggtta tttcacagcg attggaaaag caccgctgac agcaattatt ttagtgacag
    54961 aaatggttgg ctctcttaat catctgatgc ctttaggatt ggctgctctt gttgcttata
    55021 tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc attattggaa cgtctacttg
    55081 gaaatactca gaccgcctca ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg
    55141 cagagagttc tttagacgga ttaatggtac gagacttcaa ttggcctaaa aatatgctgc
    55201 taatctctat acgcagagga aatcaggaaa tcttgactca cggtgatacg atcatgaatg
    55261 taggagatat tttggttatc ttgactgatg aagcaaactt gccaagaatc aagcaggaaa
    55321 tcgaggctaa atcttctatc acaaatttga agcaatcatt ttaacgaaca tggaaaaact
    55381 attttacaac aactattcat ctacactact tgattctata tgattctata aaaaaagaag
    55441 aggctgggaa aacccagcct cttctttttt atttctatgc ttctgctact gcaggagctt
    55501 ttctcaataa accataaatc acaccagata cgactgctcc gatagcaata aacaccaaat
    55561 acatgaccgg gtgactcagt aagaatacaa cgaaaatccc gccgtgagga gccagtaatt
    55621 tgattccgaa agctccgact aatccgccag ttaaagcaga accaataata aaacttggaa
    55681 tggcacgaat cgggtcagct gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc
    55741 ccatcacgat gttcgtcaaa ccagcatctt gatctgtttt gctgaattta ttcttaaaca
    55801 aacgtgtagc aacgaaaatt gccaatggag gtaccattcc tccagccata acagaagcca
    55861 tcacgacact tccgccagtt gcaacagtcg tagctaaagt tgctgtacca aacacataag
    55921 cggctttgtt gattggaccg cctaaatctg ccgccatcat tccagctaat aatgcaccaa
    55981 gtaaagcagc attcgaacca ctcatacttg tcaagaaacc gttcaatgca tcattgattg
    56041 ctttcatagg gacattgacc agcaacatca agaaaccagt aatcagtaag ccaaacactg
    56101 gataaaagag gatcgtttta attccatcca atgattttgg taagttcttg aataatttac
    56161 gtaaaaatac gatgacataa ccagctaaga atccaccaat cagtgcacct aagaatcctg
    56221 caccgcctgt attagctaat gcaccagcag caaatccaac gatcaaacca gggcggtcac
    56281 cgatacttga agcaataaat cctgctaata ccggcagcat gaaaccaaat gcagcttgtc
    56341 cgatttggtt gaaccagcta gcagcttgat tgtaattccc tagatttgat aattgatcat
    56401 gtggcacgcc aataaattgg tcgatcatga aagaaagcgc aatagcgatt cctccaccaa
    56461 taacgaaagg taacatgtgt gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga
    56521 tagtaccatc cgcatttcct tcttctgaac tgctttcttt tccatcacca tggaatactg
    56581 gtgcagtacc actaagggct agattgatca actcttctgg tttacggata ccatcactga
    56641 caggacgatt gatcagttct tttccgtcaa agcgattcat ctctactttt ttgtctgctg
    56701 caacgattac accttctgca cgggcgatgt cttcagcagt caatcggttt ttgattcctt
    56761 ctgaaccgtt cgtttcgact ttgatctcca cacccatttc tttcgctttt ttcttcaaag
    56821 catcttctgc catgtaagtg tgagcgatcc ctgttggaca agctgtaacg gcaacaacga
    56881 attttctttc tggatgagca gaagcttctg cagcttgtgt ttcttgttct gctttttctt
    56941 ctgcttcttt ttgctcttca gctgtttgga acaagtcttg gacttcttct ggtgtatttg
    57001 cttcttttaa ttttcctacg aaatctgcat caatcagcaa tcgagatagt gctgctaatg
    57061 cttgcaaatg tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac agataagttg
    57121 gctgtccgtc taaggcttca taatctactc ctttttggct ttttgcgaat aaaactgttg
    57181 cttctttaac tgcactgttt ttcgcgtgag gcatggcgat tccatcacca agacctgtag
    57241 aagtttgtgc ttcgcgggct agaattcctt ctttatatgt ttcgatatcg gaaatccggc
    57301 cggcatcaaa cattttttgg accatttcat cgattgcgcc ttttttatca gtcgcttgca
    57361 aatccatgat catcgcttct ttgaccatta aatccttgat attcatgatt atcctccttg
    57421 aaactaatca atttttgtga tttcaacttc tggcagcaac tcatcaatca attcgcgagc
    57481 tgccaagtca tcagaaaaag ctgttgcgct tccgcaagca actccccatt taaaggcttc
    57541 gattggatct ttcgtacggc tgtaagcccc aataaagcca gcaatcattg aatcccctgc
    57601 acctactgaa tttttcaatg ggcgaactaa tacattcgtt ttataagttc cttgttccgt
    57661 gaagagcaaa gcaccgtctc cagccattga gatcagcaca ttctgtgccc cttcagctag
    57721 taattttttc ccatacggaa tgatttcttc tacagattgg aaggttgtgt tgtaaagctc
    57781 agctaattcg tgattgtttg gtttgatcaa caacggtttc ttaacaagtg cattttttag
    57841 gtcatcacct gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga tatcaatcag
    57901 ttcttgataa aatccttttc gcaggctagc tggcgcgctt cctgacaata cgacgatatc
    57961 tccttgtcct actgttccta atgcttgctt caactcatcg atttcttcat ctgaaataga
    58021 tgggcctaag ccattgatct cagtttcagt gtctgatttt agtttgatat tgattcttgt
    58081 atcttctttt acggttgtga acaaagactg gatttgttct ttttcaagcc acttcgaaat
    58141 aaattcgcca gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat cgatccgttt
    58201 caaaatacgg gaaacattga tcccttttcc tccaggtaac ttgaagtctt ccttcattcg
    58261 gttcaaatcg cctagtttta acccatctac gcgtacgata taatcaattg atggattaag
    58321 tgttactgtg taaatcattg tattgcctcc ttaattgttg tcttttcacg gaattcatta
    58381 acgaattcta atggacaacg ctcggttatg atcgtggctg cttccaaagg tgcaacagag
    58441 gtgaaggaca actggttgaa tttggtatga tcaatcaata caaatgtctc ttcggaattt
    58501 ttcatggcta aacgtttaac agctgcctct tctggatccg gagtcgtgaa tccttgttca
    58561 agatgggcac cgttcattcc cataaatgct ttattaaaat gaaaattttt tagttgttct
    58621 atcgcattag atcctaaaac agcattagtt gccagcttta tcgttccacc taaaataatg
    58681 gtagcaagct ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt cactactgtg
    58741 atctgttttc cttgcagaaa aggaatcatt tctaacgtag tagagccagc gtctaaataa
    58801 atgacatctt cgtcattaat ggtttcagct gccgatttgg cgatttgctg tttttcgtga
    58861 acgtttttga gtgatttttc actcatattc tgctcaaaat tcaataattg ctctttctta
    58921 gctccgccat gaactctttc tagaaaccca ctattctcca gctcttgtaa atcacgtctt
    58981 atcgttgatt cagaagcatt caaaaggttt acaagatctt gtgatttcac gattttctgc
    59041 tgttcaagca agtctaggat tttttgtctg cgttcctctg taagcatttt cacgatcacc
    59101 tcaatacaaa taatagcata ttttttttct actagcaata taaatcagtc aaaaacgttc
    59161 ataaaaatta taaatcagtc aaaaaaaaat aaaaaaccag tcagaaagac tggtttttta
    59221 tttttttgtc tatcatcaag ccgttttatc gttttttatt ttcgcttctt gtttttttca
    59281 tataaacaga agcaggtttt ctttccttat gaagaagata ataataaagt cccatcccgc
    59341 caaacatgac caaaaaaccg atgactgatc gaataggaaa attaggaaca aaaaagagac
    59401 agacggataa taatgatgcc ggaaccatta ttcgataata gagttgttta tccattttct
    59461 tcacctcata ttttttgtaa agtaatcaat tgttctaatt ctcggtaatc taaaaattgc
    59521 acacgcagat gctgctcacc tttcttgaca tcgaattgga tacttcgccg ctttttcttt
    59581 attcgtttga aggtaacatc agagagtcta tgtttgattt cctttaacgg taattctgac
    59641 agggattctg aaggaacata gattcttaag gataaaagat actcttgata tttttcatct
    59701 gttacctcta cttctagata ttccctaata taataacaat aagcttcgat tccattatcc
    59761 aaagcaacag tcggatcgat aactttatag cacatcatgc gtgatcgatt ctcgtcaacc
    59821 caaggaaaat acgcctgagc atttagtgtt ccgcgaacac gcctttcttt ataatctcct
    59881 tgttggatat cataactttt tgttacaaaa aatgaagcat tctttttagg aacaggttgc
    59941 atttgtgcat tctgatactg gacagattgg atcgtatggt cagtgtaaat gtattcaacg
    60001 aatggcggtt tcaataaacc gacaccattg tcccaagcaa gatgatcaaa cccaagctgg
    60061 tccctaaaat aggattgaaa catttgattg tttggctttt gccagttaaa ggggtctcga
    60121 taaagctgga tacatatgac ttcgttttct aattccttca ctttttctac ttgttggatg
    60181 tcgagaaaaa tcggtttagg gatgatttga tgataatagc gactataata catttcccaa
    60241 cacgaagtga aacataaacc ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg
    60301 actactactt ccccgttttg ttgtttcatt ttaggtaaat gttcgatttc atcctttttc
    60361 tcaaacaata gccgttgaga gatctgtttt gtattgtgat agataaactc gctataagct
    60421 cgcatatagg cgaacacccc atgagcaatc atttttttct gaatgaagtc tcgaatcaaa
    60481 aattgctttt ctttgaaaac agagatagac aatacatttt ttatcaataa ttgattatct
    60541 tccaagcgaa aactacattc gttctcgtca tttcctacaa ctaaccaact ttcgtctctt
    60601 accgatgcta gcattatttt ttcttgaagc tttcgaagat catacggatc cgcttgttgt
    60661 gtgaaagagc ggataaatgt tggctggatc aacagacgtg tcaaaatagc ccaatcgctt
    60721 atttcctgtt ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta
    60781 tcttcgccaa tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg
    60841 ataaagaaaa actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact
    60901 aagtaaaaag aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt
    60961 tagataaact tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag
    61021 agatcgatcg atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa
    61081 agtaagttaa gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt
    61141 aagtcttttt tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc
    61201 ttttccctgt tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa
    61261 cgcttttttg atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt
    61321 agtggcaaca acaattacag ggatatcata atacttcaaa aattgataca tctggatatc
    61381 ttcttgagaa ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct
    61441 agtcgttaaa tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt
    61501 tgcataccca tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt
    61561 caatgtctgg gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa
    61621 tgtgttgata aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa
    61681 ttgtgtatct ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac
    61741 tttcatggtc tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca
    61801 aaatgacttt tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga
    61861 ttactgttgg ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt
    61921 tttcatttga agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa
    61981 gtcctcgagc accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag
    62041 gttcgaattc aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg
    62101 catttttcgg ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg
    62161 ccataacagg taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag
    62221 gaatgatatg ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc
    62281 caatcgtctt ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac
    62341 ctacgatgaa caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac
    62401 gtccgccctg aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt
    62461 ggacaccttc gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt
    62521 tatcgatctc atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag
    62581 attgaagcaa tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca
    62641 aacttgtcgc atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta
    62701 aaaatgtttt accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa
    62761 catcatctgt gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa
    62821 cagctaacga gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa
    62881 tttctagtgg tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat
    62941 caatgatttc tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta
    63001 cgatcttttt cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc
    63061 tacttggatt gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta
    63121 tcaaagactt tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta
    63181 acaaaggatc aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact
    63241 gtgacaaaat cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct
    63301 tcagaaatga tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca
    63361 attcgattgc ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt
    63421 tgatttgttc gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt
    63481 cttcttctgt tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc
    63541 ttgtgcgcat ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag
    63601 ttaattgata gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt
    63661 ccattgaacg atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt
    63721 tttcaacagc catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg
    63781 tttctactaa ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat
    63841 caacatcttt tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga
    63901 ttgtcacttt gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga
    63961 aagttacatt tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac
    64021 ctggaatgaa tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa
    64081 acgcttcgcc atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg
    64141 cagcatcttc tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat
    64201 ccacgtcagc atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc
    64261 ctaattttac ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt
    64321 ccatgctttc aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag
    64381 cagcttcata agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac
    64441 cgtacatacg gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca
    64501 agcttttttt cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa
    64561 tcgtaaattt taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat
    64621 tccctccgag tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat
    64681 agtacactaa ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata
    64741 acatttttat ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt
    64801 tttctctttt ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc
    64861 ctgtacacgc ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat
    64921 agacaggata aacaattgca gattgcaacg tgaattcttc
//
LOCUS       DACERL010000007        59896 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.007, whole genome shotgun sequence.
ACCESSION   DACERL010000007 DACERL010000000
VERSION     DACERL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59896
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.007"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(437..1933)
                     /gene="lysS"
                     /locus_tag="IXF54_000439"
     CDS             complement(437..1933)
                     /gene="lysS"
                     /locus_tag="IXF54_000439"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1403724.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2044..3048)
                     /gene="dusB"
                     /locus_tag="IXF54_000440"
     CDS             complement(2044..3048)
                     /gene="dusB"
                     /locus_tag="IXF54_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1403725.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3049..3936)
                     /gene="hslO"
                     /locus_tag="IXF54_000441"
     CDS             complement(3049..3936)
                     /gene="hslO"
                     /locus_tag="IXF54_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1403726.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4153..6264)
                     /gene="hflB"
                     /locus_tag="IXF54_000442"
     CDS             complement(4153..6264)
                     /gene="hflB"
                     /locus_tag="IXF54_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1403727.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6354..6899)
                     /gene="hpt"
                     /locus_tag="IXF54_000443"
     CDS             complement(6354..6899)
                     /gene="hpt"
                     /locus_tag="IXF54_000443"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1403728.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6899..8344)
                     /gene="tilS"
                     /locus_tag="IXF54_000444"
     CDS             complement(6899..8344)
                     /gene="tilS"
                     /locus_tag="IXF54_000444"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1403729.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8464..8934)
                     /locus_tag="IXF54_000445"
     CDS             complement(8464..8934)
                     /locus_tag="IXF54_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1403730.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8980..9405)
                     /locus_tag="IXF54_000446"
     CDS             complement(8980..9405)
                     /locus_tag="IXF54_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1403731.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9472..9741)
                     /locus_tag="IXF54_000447"
     CDS             complement(9472..9741)
                     /locus_tag="IXF54_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1403732.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9752..11347)
                     /locus_tag="IXF54_000448"
     CDS             complement(9752..11347)
                     /locus_tag="IXF54_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1403733.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11362..14883)
                     /gene="mfd"
                     /locus_tag="IXF54_000449"
     CDS             complement(11362..14883)
                     /gene="mfd"
                     /locus_tag="IXF54_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1403734.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14900..15460)
                     /locus_tag="IXF54_000450"
     CDS             complement(14900..15460)
                     /locus_tag="IXF54_000450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1403735.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15813..16787
                     /locus_tag="IXF54_000451"
     CDS             15813..16787
                     /locus_tag="IXF54_000451"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1403736.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16957..18357
                     /locus_tag="IXF54_000452"
     CDS             16957..18357
                     /locus_tag="IXF54_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1403737.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18435..19157)
                     /locus_tag="IXF54_000453"
     CDS             complement(18435..19157)
                     /locus_tag="IXF54_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1403738.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19236..19742)
                     /locus_tag="IXF54_000454"
     CDS             complement(19236..19742)
                     /locus_tag="IXF54_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1403739.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19865..21664
                     /locus_tag="IXF54_000455"
     CDS             19865..21664
                     /locus_tag="IXF54_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1403740.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21732..23306)
                     /locus_tag="IXF54_000456"
     CDS             complement(21732..23306)
                     /locus_tag="IXF54_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403741.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23561..24070)
                     /gene="mreD"
                     /locus_tag="IXF54_000457"
     CDS             complement(23561..24070)
                     /gene="mreD"
                     /locus_tag="IXF54_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1403742.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24074..24928)
                     /gene="mreC"
                     /locus_tag="IXF54_000458"
     CDS             complement(24074..24928)
                     /gene="mreC"
                     /locus_tag="IXF54_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1403743.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25083..25721)
                     /locus_tag="IXF54_000459"
     CDS             complement(25083..25721)
                     /locus_tag="IXF54_000459"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1403744.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25722..26372)
                     /locus_tag="IXF54_000460"
     CDS             complement(25722..26372)
                     /locus_tag="IXF54_000460"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1403745.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26385..27284)
                     /gene="rsgA"
                     /locus_tag="IXF54_000461"
     CDS             complement(26385..27284)
                     /gene="rsgA"
                     /locus_tag="IXF54_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1403746.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27449..29518)
                     /gene="pknB"
                     /locus_tag="IXF54_000462"
     CDS             complement(27449..29518)
                     /gene="pknB"
                     /locus_tag="IXF54_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1403747.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29515..30255)
                     /locus_tag="IXF54_000463"
     CDS             complement(29515..30255)
                     /locus_tag="IXF54_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1403748.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30268..31626)
                     /gene="rsmB"
                     /locus_tag="IXF54_000464"
     CDS             complement(30268..31626)
                     /gene="rsmB"
                     /locus_tag="IXF54_000464"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1403749.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31626..32564)
                     /locus_tag="IXF54_000465"
     CDS             complement(31626..32564)
                     /locus_tag="IXF54_000465"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1403750.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32557..33048)
                     /gene="def"
                     /locus_tag="IXF54_000466"
     CDS             complement(32557..33048)
                     /gene="def"
                     /locus_tag="IXF54_000466"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1403751.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33063..35471)
                     /gene="priA"
                     /locus_tag="IXF54_000467"
     CDS             complement(33063..35471)
                     /gene="priA"
                     /locus_tag="IXF54_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1403752.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35724..36029)
                     /locus_tag="IXF54_000468"
     CDS             complement(35724..36029)
                     /locus_tag="IXF54_000468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1403753.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36034..36648)
                     /gene="gmk"
                     /locus_tag="IXF54_000469"
     CDS             complement(36034..36648)
                     /gene="gmk"
                     /locus_tag="IXF54_000469"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1403754.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37135..37419)
                     /locus_tag="IXF54_000470"
     CDS             complement(37135..37419)
                     /locus_tag="IXF54_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403755.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37437..38327)
                     /locus_tag="IXF54_000471"
     CDS             complement(37437..38327)
                     /locus_tag="IXF54_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1403756.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38347..38565)
                     /locus_tag="IXF54_000472"
     CDS             complement(38347..38565)
                     /locus_tag="IXF54_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403757.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38535..40235)
                     /locus_tag="IXF54_000473"
     CDS             complement(38535..40235)
                     /locus_tag="IXF54_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1403758.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40502..41275
                     /locus_tag="IXF54_000474"
     CDS             40502..41275
                     /locus_tag="IXF54_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403759.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41356..41925)
                     /gene="thiT"
                     /locus_tag="IXF54_000475"
     CDS             complement(41356..41925)
                     /gene="thiT"
                     /locus_tag="IXF54_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1403760.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41991..42093)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42099..43070)
                     /locus_tag="IXF54_000476"
     CDS             complement(42099..43070)
                     /locus_tag="IXF54_000476"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1403761.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43164..44537)
                     /gene="glmU"
                     /locus_tag="IXF54_000477"
     CDS             complement(43164..44537)
                     /gene="glmU"
                     /locus_tag="IXF54_000477"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1403762.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45040..45858)
                     /gene="purR"
                     /locus_tag="IXF54_000478"
     CDS             complement(45040..45858)
                     /gene="purR"
                     /locus_tag="IXF54_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1403763.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(46025..46849)
                     /locus_tag="IXF54_000479"
     CDS             complement(46025..46849)
                     /locus_tag="IXF54_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1403764.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46822..47502)
                     /locus_tag="IXF54_000480"
     CDS             complement(46822..47502)
                     /locus_tag="IXF54_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403765.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47525..48463)
                     /locus_tag="IXF54_000481"
     CDS             complement(47525..48463)
                     /locus_tag="IXF54_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1403766.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48708..49559)
                     /locus_tag="IXF54_000482"
     CDS             complement(48708..49559)
                     /locus_tag="IXF54_000482"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1403767.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49713..52286)
                     /gene="mprF"
                     /locus_tag="IXF54_000483"
     CDS             complement(49713..52286)
                     /gene="mprF"
                     /locus_tag="IXF54_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1403768.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52427..53233
                     /gene="proC"
                     /locus_tag="IXF54_000484"
     CDS             52427..53233
                     /gene="proC"
                     /locus_tag="IXF54_000484"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1403769.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53312..54868)
                     /gene="rny"
                     /locus_tag="IXF54_000485"
     CDS             complement(53312..54868)
                     /gene="rny"
                     /locus_tag="IXF54_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1403770.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55279..56328)
                     /gene="recA"
                     /locus_tag="IXF54_000486"
     CDS             complement(55279..56328)
                     /gene="recA"
                     /locus_tag="IXF54_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1403771.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56437..57678)
                     /locus_tag="IXF54_000487"
     CDS             complement(56437..57678)
                     /locus_tag="IXF54_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1403772.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57918..58496)
                     /gene="pgsA"
                     /locus_tag="IXF54_000488"
     CDS             complement(57918..58496)
                     /gene="pgsA"
                     /locus_tag="IXF54_000488"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1403773.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58705..59589)
                     /locus_tag="IXF54_000489"
     CDS             complement(58705..59589)
                     /locus_tag="IXF54_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1403774.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT        16510 a        13043 c        10726 g        19617 t
ORIGIN      
        1 cgatttcgtt tttcgaaatc tttcggcaac tcatttatca taacttatcg tttgttattt
       61 gtcaagaact tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt
      121 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
      181 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      241 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      301 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      361 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      421 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      481 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      541 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      601 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      661 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      721 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      781 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      841 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      901 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      961 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
     1021 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
     1081 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1141 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1201 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1261 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1321 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1381 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1441 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1501 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1561 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1621 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1681 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1741 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1801 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1861 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1921 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1981 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     2041 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     2101 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2161 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2221 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2281 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2341 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2401 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2461 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2521 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2581 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2641 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2701 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2761 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2821 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2881 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2941 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     3001 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     3061 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3121 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3181 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3241 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3301 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3361 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3421 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3481 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3541 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3601 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3661 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3721 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3781 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3841 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3901 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3961 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     4021 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     4081 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4141 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4201 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4261 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4321 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4381 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4441 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4501 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4561 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4621 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4681 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4741 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4801 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4861 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4921 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4981 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     5041 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     5101 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5161 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5221 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5281 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5341 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5401 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5461 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5521 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5581 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5641 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5701 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5761 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5821 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5881 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5941 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     6001 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     6061 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6121 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6181 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6241 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6301 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6361 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6421 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6481 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6541 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6601 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6661 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6721 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6781 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6841 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6901 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6961 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     7021 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     7081 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7141 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7201 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7261 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7321 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7381 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7441 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7501 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7561 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7621 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7681 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7741 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7801 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7861 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7921 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7981 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     8041 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     8101 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8161 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8221 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8281 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8341 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8401 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8461 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8521 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8581 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8641 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8701 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8761 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8821 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8881 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8941 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     9001 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     9061 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9121 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9181 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9241 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9301 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9361 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9421 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9481 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9541 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9601 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9661 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9721 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9781 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9841 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9901 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9961 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
    10021 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
    10081 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10141 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10201 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10261 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10321 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10381 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10441 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10501 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10561 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10621 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10681 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10741 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10801 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10861 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10921 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10981 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    11041 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    11101 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11161 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11221 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11281 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11341 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11401 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11461 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11521 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11581 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11641 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11701 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11761 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11821 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11881 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11941 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    12001 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    12061 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12121 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12181 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12241 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12301 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12361 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12421 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12481 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12541 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12601 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12661 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12721 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12781 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12841 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12901 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12961 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    13021 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    13081 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13141 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13201 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13261 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13321 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13381 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13441 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13501 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13561 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13621 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13681 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13741 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13801 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13861 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13921 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13981 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    14041 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    14101 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14161 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14221 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14281 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14341 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14401 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14461 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14521 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14581 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14641 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14701 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14761 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14821 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14881 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14941 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    15001 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    15061 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15121 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15181 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15241 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15301 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15361 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15421 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15481 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15541 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15601 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15661 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15721 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15781 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15841 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15901 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15961 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    16021 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    16081 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16141 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16201 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16261 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16321 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16381 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16441 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16501 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16561 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16621 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16681 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16741 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16801 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16861 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16921 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16981 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    17041 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    17101 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17161 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17221 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17281 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17341 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17401 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17461 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17521 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17581 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17641 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17701 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17761 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17821 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17881 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17941 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    18001 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    18061 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18121 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18181 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18241 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18301 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18361 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18421 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18481 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18541 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18601 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18661 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18721 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18781 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18841 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18901 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18961 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    19021 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    19081 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19141 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19201 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19261 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19321 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19381 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19441 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19501 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19561 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19621 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19681 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19741 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19801 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19861 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19921 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19981 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    20041 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    20101 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20161 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20221 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20281 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20341 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20401 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20461 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20521 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20581 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20641 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20701 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20761 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20821 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20881 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20941 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    21001 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    21061 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21121 cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21181 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21241 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21301 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21361 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21421 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21481 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21541 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21601 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21661 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21721 attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact
    21781 tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct
    21841 acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa
    21901 actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca
    21961 gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta
    22021 ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca
    22081 ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt
    22141 gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct
    22201 gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact
    22261 gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa
    22321 gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct
    22381 gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat
    22441 tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta
    22501 gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct
    22561 ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga
    22621 gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca
    22681 gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa
    22741 agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca
    22801 tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag
    22861 tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat
    22921 gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg
    22981 cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg
    23041 tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt
    23101 gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct
    23161 gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa
    23221 tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt
    23281 actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat
    23341 atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa
    23401 cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt
    23461 tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa
    23521 cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt
    23581 tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta
    23641 aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca
    23701 ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga
    23761 tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta
    23821 ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg
    23881 tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca
    23941 gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat
    24001 gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt
    24061 tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat
    24121 acatttctgc ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg
    24181 ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct
    24241 ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat
    24301 ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc
    24361 gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag
    24421 ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg
    24481 cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg
    24541 aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa
    24601 gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat
    24661 catagctatc gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat
    24721 ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca
    24781 ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct
    24841 gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa
    24901 tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt
    24961 tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt
    25021 tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg
    25081 gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta
    25141 tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat
    25201 acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa
    25261 tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa
    25321 ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct
    25381 aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact
    25441 tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct
    25501 ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg
    25561 ttcgacaatg aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg
    25621 tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc
    25681 cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt
    25741 aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag
    25801 acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact
    25861 tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct
    25921 actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact
    25981 tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact
    26041 actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt
    26101 gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt
    26161 tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc
    26221 tgaacaatat ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg
    26281 tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa
    26341 tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct
    26401 tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg
    26461 tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt
    26521 tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga
    26581 aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt
    26641 gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt
    26701 cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga
    26761 ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg
    26821 aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct
    26881 attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc
    26941 tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca
    27001 agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca
    27061 tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg
    27121 tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga
    27181 ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa
    27241 aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat
    27301 tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct
    27361 tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca
    27421 ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg
    27481 aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg
    27541 agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt
    27601 tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg
    27661 tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat
    27721 attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat
    27781 cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta
    27841 caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat
    27901 agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa
    27961 cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga
    28021 ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg
    28081 aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag
    28141 cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat
    28201 catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct
    28261 tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat
    28321 ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag
    28381 cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag
    28441 caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc
    28501 attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt
    28561 cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat
    28621 cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt
    28681 cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg
    28741 acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt
    28801 gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag
    28861 caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga
    28921 tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag
    28981 gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata
    29041 aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt
    29101 tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa
    29161 ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt gtttggatat
    29221 atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt
    29281 caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt
    29341 ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa
    29401 ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc
    29461 taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac
    29521 actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt
    29581 ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt
    29641 tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc
    29701 ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga
    29761 acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga
    29821 tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg
    29881 aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc
    29941 ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata
    30001 aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc
    30061 aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat
    30121 gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg
    30181 gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg
    30241 atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc
    30301 atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag
    30361 cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga
    30421 aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga
    30481 agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc
    30541 taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc
    30601 tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac
    30661 ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt
    30721 atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca
    30781 tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc
    30841 taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc
    30901 ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc
    30961 tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac
    31021 agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg
    31081 ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg
    31141 tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat
    31201 gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt
    31261 ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg
    31321 tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac
    31381 ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg
    31441 ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag
    31501 ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac
    31561 tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt
    31621 tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca
    31681 tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt
    31741 ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg
    31801 tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc
    31861 gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt
    31921 ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac
    31981 tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc
    32041 tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt
    32101 ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc
    32161 gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga
    32221 tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac
    32281 ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg
    32341 atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc
    32401 ttcagagccg ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg
    32461 tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc
    32521 ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat
    32581 atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc
    32641 gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc
    32701 tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt
    32761 tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc
    32821 gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat
    32881 tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt
    32941 ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc
    33001 agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt
    33061 tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct
    33121 ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat
    33181 ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc
    33241 cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt
    33301 ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa
    33361 ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt
    33421 tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg
    33481 cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc
    33541 tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat
    33601 gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct
    33661 tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct
    33721 acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt
    33781 tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga
    33841 ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta
    33901 agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa
    33961 gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt
    34021 tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca
    34081 cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac
    34141 tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg
    34201 cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca
    34261 tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc
    34321 agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct
    34381 tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt
    34441 aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat
    34501 gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc
    34561 tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct
    34621 ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt
    34681 tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat
    34741 tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct
    34801 ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt
    34861 tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca
    34921 gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat
    34981 ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct
    35041 tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg
    35101 tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt
    35161 tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt
    35221 aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga
    35281 ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca
    35341 ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga
    35401 accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct
    35461 gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt
    35521 ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa
    35581 aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc
    35641 taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta
    35701 gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat
    35761 tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca
    35821 cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct
    35881 tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat
    35941 tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat
    36001 agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata
    36061 tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc
    36121 aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat
    36181 ttcttctttt gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc
    36241 tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc
    36301 gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt
    36361 tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata
    36421 ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg
    36481 gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat
    36541 cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc
    36601 tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt
    36661 tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt
    36721 tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa
    36781 aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg
    36841 tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga
    36901 agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt
    36961 gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa
    37021 aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta
    37081 agcgaagaaa tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa
    37141 gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt
    37201 ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt
    37261 cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc
    37321 atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt
    37381 tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat
    37441 tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga
    37501 tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct
    37561 tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt
    37621 ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag
    37681 atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc
    37741 tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt
    37801 tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct
    37861 tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac
    37921 tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc
    37981 agcacgaagc gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt
    38041 tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc
    38101 tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt
    38161 aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt
    38221 cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc taactgatac
    38281 gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct
    38341 tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt
    38401 caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg
    38461 gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa
    38521 tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg
    38581 agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc
    38641 aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca
    38701 ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca
    38761 atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc
    38821 atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct
    38881 agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt
    38941 tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa
    39001 gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc
    39061 ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg
    39121 ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa
    39181 tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg
    39241 acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac
    39301 ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa
    39361 gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact
    39421 acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt
    39481 ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc
    39541 ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga
    39601 aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc
    39661 cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct
    39721 accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca
    39781 ccaatctgat aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc
    39841 agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat
    39901 tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg
    39961 caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga
    40021 ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc
    40081 gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct
    40141 tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac
    40201 attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat
    40261 accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa
    40321 acaattcata aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa
    40381 tgagagtgtt ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag
    40441 cttttttaat gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat
    40501 gatgactgag tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg
    40561 gctgactgct actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct
    40621 tgatccatct gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg
    40681 tacagaaaat attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt
    40741 gaagtggatc gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc
    40801 agaattatta tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact
    40861 acagaataat tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta
    40921 tttagatgaa ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat
    40981 caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt
    41041 attgaaagaa atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg
    41101 tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt
    41161 aaaaggcacc cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga
    41221 tcgacactgg caattggtag aagatgccga tctttcttat ctgccattga agtagatcta
    41281 tttcttatca aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc
    41341 ttgcttcttt tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt
    41401 ttgttggatc cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc
    41461 attcattacg aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa
    41521 aaccactcct gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa
    41581 cacgatgctt tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga
    41641 agccatgagc cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg
    41701 aacgacactt aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc
    41761 tgccagcatc cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc
    41821 taatgaaata gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa
    41881 cgctgcaaca atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc
    41941 tttgaataaa aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta
    42001 ttcacaattc ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa
    42061 tctcagccca attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac
    42121 aatggactaa ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca
    42181 acacttactt catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta
    42241 acaactagac gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact
    42301 ggatgtgtac atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc
    42361 aatgtgatcg tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg
    42421 acttgtccaa tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg
    42481 atcgcaatcg gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg
    42541 tctggtgaaa caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca
    42601 ataagtggcg cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact
    42661 gcatgaaggt ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact
    42721 aatttcgctg tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa
    42781 taaggcatga ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc
    42841 agcaactcca tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc
    42901 gatccacgaa tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca
    42961 gatgattttc ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg
    43021 gaatttaacg caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc
    43081 actttctttt tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata
    43141 aatcaagtga ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct
    43201 ttattgactt gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt
    43261 gttgacccgg cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg
    43321 ctagaaccaa taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag
    43381 ttgacgaaga caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca
    43441 taagtcaagt gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag
    43501 tttccgatat ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc
    43561 acatctgcat gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga
    43621 tcctcaatcc tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg
    43681 actgtatttc cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg
    43741 cctgcatcga tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc
    43801 tggttaattc gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact
    43861 cccattgatt cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt
    43921 aagatttcga tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg
    43981 cttaacgcat cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt
    44041 acaagagctt cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata
    44101 tggtcacgga tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt
    44161 gcacttgcat tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat
    44221 aatggtgtat cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca
    44281 gcttgcaaaa cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt
    44341 tctcctagtt gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct
    44401 ggtttcgttt cactgacccg attaatgata tgttctacca taggtttacc agaaacggga
    44461 tgtaaaactt tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt
    44521 gcataacgtg cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc
    44581 agtataccca ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata
    44641 aaaaaataga tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact
    44701 cctcggtcac aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct
    44761 tgtgacttag agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct
    44821 gaccttcggt attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga
    44881 gctaatactc aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc
    44941 agtttctcgg tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt
    45001 ttcttactac tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc
    45061 tggcacaacc gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt
    45121 ataatcatcg atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac
    45181 aagttctgat tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt
    45241 catgaaatca tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc
    45301 catcttttca attctttcag aagatccaga cacatagttg acacttactg ttgatccttc
    45361 tgttattttt gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc
    45421 ttgggcaata ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag
    45481 gtatttagaa gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc
    45541 agagagataa acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag
    45601 agattcaata tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc
    45661 tgcagcaccc gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat
    45721 gattcccaaa tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt
    45781 taaggaaatc aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc
    45841 actgcgacgt actttcacct attccaactc cttttaagaa attttatcgt tttacattat
    45901 ataagttttt cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt
    45961 tattaaaata aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta
    46021 taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca
    46081 ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg
    46141 agctagttag tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga
    46201 cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata
    46261 tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga
    46321 acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag
    46381 tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga
    46441 ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt
    46501 ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac
    46561 caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat
    46621 actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt
    46681 tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca
    46741 gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga
    46801 tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc
    46861 ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt
    46921 gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc
    46981 atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc
    47041 agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat
    47101 tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg
    47161 atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa
    47221 ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc
    47281 gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg
    47341 tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt
    47401 taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta caggttcatc
    47461 atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta
    47521 aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat
    47581 tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct
    47641 tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat
    47701 aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg
    47761 tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg
    47821 ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct
    47881 catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg
    47941 taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt
    48001 cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat
    48061 gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt
    48121 tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa
    48181 aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt
    48241 cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt
    48301 ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga
    48361 tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta
    48421 aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa
    48481 tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca
    48541 cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa
    48601 aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc
    48661 attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt
    48721 cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt
    48781 gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct
    48841 gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt
    48901 atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg
    48961 gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac
    49021 actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata
    49081 gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca
    49141 cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag
    49201 cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg
    49261 cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata
    49321 ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt
    49381 aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga
    49441 tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt
    49501 ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc
    49561 ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta
    49621 tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt
    49681 ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc
    49741 aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat
    49801 ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt
    49861 aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata
    49921 tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct
    49981 aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac
    50041 agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc
    50101 tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca
    50161 ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt
    50221 tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc
    50281 ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt
    50341 tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact
    50401 ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa
    50461 aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc
    50521 atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga
    50581 taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac
    50641 aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg
    50701 tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc
    50761 actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa
    50821 ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag
    50881 cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct
    50941 ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac
    51001 agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa
    51061 ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt
    51121 gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa
    51181 ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt
    51241 gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc
    51301 gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct
    51361 gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga
    51421 taatataata gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag
    51481 atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat
    51541 gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg
    51601 ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga
    51661 ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa
    51721 aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa
    51781 atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa
    51841 ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc
    51901 tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga
    51961 ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt
    52021 tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca
    52081 cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt
    52141 tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag
    52201 actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc
    52261 agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc
    52321 ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc
    52381 gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt
    52441 tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt
    52501 ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc
    52561 tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt
    52621 tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag
    52681 ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc
    52741 gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg
    52801 agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc
    52861 tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac
    52921 attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg
    52981 acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc
    53041 aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga
    53101 ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg
    53161 attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt
    53221 gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc
    53281 ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt
    53341 cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac
    53401 gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta
    53461 cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg
    53521 aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac
    53581 caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag
    53641 cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact
    53701 tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt
    53761 tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg
    53821 ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat
    53881 aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag
    53941 caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac
    54001 gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt
    54061 tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg
    54121 cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat
    54181 tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg
    54241 aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt
    54301 ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag
    54361 ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt
    54421 ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt
    54481 ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa
    54541 gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa
    54601 tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt
    54661 cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt
    54721 ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt
    54781 ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga
    54841 tgatagcgag gagaatgttg aataccataa aatccacctc catactatct ttttcatttt
    54901 tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat
    54961 atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact
    55021 ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat
    55081 gacactagtt tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt
    55141 tgtagatgaa cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat
    55201 ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt
    55261 ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc
    55321 ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc
    55381 tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat
    55441 ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc
    55501 catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc
    55561 gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac
    55621 aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta
    55681 gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca cgcctacttt
    55741 ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag
    55801 tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc
    55861 gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac
    55921 agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg
    55981 tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc
    56041 atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg
    56101 tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg
    56161 ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga
    56221 gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc
    56281 aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct
    56341 ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa
    56401 aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct
    56461 caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa
    56521 gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc
    56581 agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt
    56641 tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc
    56701 atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta
    56761 ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg
    56821 gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag
    56881 ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac
    56941 tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt
    57001 tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg
    57061 attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc
    57121 ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa
    57181 aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat
    57241 atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag
    57301 aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg
    57361 ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt
    57421 accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc
    57481 acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg
    57541 ctgattatct cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc
    57601 tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc
    57661 tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat
    57721 ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc
    57781 caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat
    57841 gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc
    57901 tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa
    57961 tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga
    58021 ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt
    58081 gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt
    58141 agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca
    58201 ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc
    58261 aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca
    58321 tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca
    58381 gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa
    58441 atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta
    58501 tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta
    58561 cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa
    58621 cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac
    58681 agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt
    58741 tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt
    58801 tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt
    58861 actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac
    58921 acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt
    58981 gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc
    59041 ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc
    59101 tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat
    59161 tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa
    59221 agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc
    59281 ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac
    59341 cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc
    59401 tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc
    59461 aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa
    59521 ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc
    59581 aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa
    59641 cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa
    59701 taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga
    59761 agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt
    59821 tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa
    59881 ggagatattt ttccgt
//
LOCUS       DACERL010000008        59883 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.008, whole genome shotgun sequence.
ACCESSION   DACERL010000008 DACERL010000000
VERSION     DACERL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59883
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.008"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(43..1083)
                     /locus_tag="IXF54_000490"
     CDS             complement(43..1083)
                     /locus_tag="IXF54_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1403775.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1360..3162)
                     /locus_tag="IXF54_000491"
     CDS             complement(1360..3162)
                     /locus_tag="IXF54_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403776.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3176..3928)
                     /locus_tag="IXF54_000492"
     CDS             complement(3176..3928)
                     /locus_tag="IXF54_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403777.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3937..4299)
                     /locus_tag="IXF54_000493"
     CDS             complement(3937..4299)
                     /locus_tag="IXF54_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1403778.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4475..5347)
                     /locus_tag="IXF54_000494"
     CDS             complement(4475..5347)
                     /locus_tag="IXF54_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1403779.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5398..5886)
                     /locus_tag="IXF54_000495"
     CDS             complement(5398..5886)
                     /locus_tag="IXF54_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1403780.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(6024..6854)
                     /locus_tag="IXF54_000496"
     CDS             complement(6024..6854)
                     /locus_tag="IXF54_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1403781.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(6870..7478)
                     /locus_tag="IXF54_000497"
     CDS             complement(6870..7478)
                     /locus_tag="IXF54_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1403782.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7471..7788)
                     /locus_tag="IXF54_000498"
     CDS             complement(7471..7788)
                     /locus_tag="IXF54_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1403783.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7944..8672)
                     /locus_tag="IXF54_000499"
     CDS             complement(7944..8672)
                     /locus_tag="IXF54_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1403784.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8831..9712
                     /locus_tag="IXF54_000500"
     CDS             8831..9712
                     /locus_tag="IXF54_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1403785.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9949..10992)
                     /locus_tag="IXF54_000501"
     CDS             complement(9949..10992)
                     /locus_tag="IXF54_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1403786.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10995..11663)
                     /locus_tag="IXF54_000502"
     CDS             complement(10995..11663)
                     /locus_tag="IXF54_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1403787.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11805..14141
                     /locus_tag="IXF54_000503"
     CDS             11805..14141
                     /locus_tag="IXF54_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403788.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14256..14567)
                     /locus_tag="IXF54_000504"
     CDS             complement(14256..14567)
                     /locus_tag="IXF54_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403789.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14748..16223
                     /locus_tag="IXF54_000505"
     CDS             14748..16223
                     /locus_tag="IXF54_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1403790.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16270..16854
                     /locus_tag="IXF54_000506"
     CDS             16270..16854
                     /locus_tag="IXF54_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1403791.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16856..17275
                     /locus_tag="IXF54_000507"
     CDS             16856..17275
                     /locus_tag="IXF54_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1403792.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17556..17840)
                     /locus_tag="IXF54_000508"
     CDS             complement(17556..17840)
                     /locus_tag="IXF54_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1403793.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18192..18902)
                     /locus_tag="IXF54_000509"
     CDS             complement(18192..18902)
                     /locus_tag="IXF54_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1403794.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18899..20371)
                     /locus_tag="IXF54_000510"
     CDS             complement(18899..20371)
                     /locus_tag="IXF54_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1403795.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20509..20982
                     /locus_tag="IXF54_000511"
     CDS             20509..20982
                     /locus_tag="IXF54_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1403796.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21135..21686)
                     /locus_tag="IXF54_000512"
     CDS             complement(21135..21686)
                     /locus_tag="IXF54_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1403797.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21791..22321
                     /locus_tag="IXF54_000513"
     CDS             21791..22321
                     /locus_tag="IXF54_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1403798.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22804..23247)
                     /locus_tag="IXF54_000514"
     CDS             complement(22804..23247)
                     /locus_tag="IXF54_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403799.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23263..24423)
                     /locus_tag="IXF54_000515"
     CDS             complement(23263..24423)
                     /locus_tag="IXF54_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1403800.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24930..26630
                     /locus_tag="IXF54_000516"
     CDS             24930..26630
                     /locus_tag="IXF54_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403801.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26732..27565)
                     /locus_tag="IXF54_000517"
     CDS             complement(26732..27565)
                     /locus_tag="IXF54_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1403802.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27869..30034)
                     /locus_tag="IXF54_000518"
     CDS             complement(27869..30034)
                     /locus_tag="IXF54_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1403803.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30294..32588
                     /locus_tag="IXF54_000519"
     CDS             30294..32588
                     /locus_tag="IXF54_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1403804.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32581..33246
                     /gene="pgmB"
                     /locus_tag="IXF54_000520"
     CDS             32581..33246
                     /gene="pgmB"
                     /locus_tag="IXF54_000520"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1403805.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33239..34246
                     /locus_tag="IXF54_000521"
     CDS             33239..34246
                     /locus_tag="IXF54_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1403806.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34268..35287
                     /locus_tag="IXF54_000522"
     CDS             34268..35287
                     /locus_tag="IXF54_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1403807.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35568..36935)
                     /gene="nhaC"
                     /locus_tag="IXF54_000523"
     CDS             complement(35568..36935)
                     /gene="nhaC"
                     /locus_tag="IXF54_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1403808.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37044..38465)
                     /locus_tag="IXF54_000524"
     CDS             complement(37044..38465)
                     /locus_tag="IXF54_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1403809.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38708..40585)
                     /gene="tdc"
                     /locus_tag="IXF54_000525"
     CDS             complement(38708..40585)
                     /gene="tdc"
                     /locus_tag="IXF54_000525"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1403810.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40876..42132)
                     /locus_tag="IXF54_000526"
     CDS             complement(40876..42132)
                     /locus_tag="IXF54_000526"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1403811.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            42712..44013
                     /locus_tag="IXF54_000527"
     CDS             42712..44013
                     /locus_tag="IXF54_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1403812.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44082..44681)
                     /locus_tag="IXF54_000528"
     CDS             complement(44082..44681)
                     /locus_tag="IXF54_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1403813.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44935..45816
                     /locus_tag="IXF54_000529"
     CDS             44935..45816
                     /locus_tag="IXF54_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403814.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45829..47439
                     /locus_tag="IXF54_000530"
     CDS             45829..47439
                     /locus_tag="IXF54_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1403815.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47516..48175)
                     /locus_tag="IXF54_000531"
     CDS             complement(47516..48175)
                     /locus_tag="IXF54_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1403816.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48458..49123)
                     /locus_tag="IXF54_000532"
     CDS             complement(48458..49123)
                     /locus_tag="IXF54_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403817.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49270..49875)
                     /gene="dhaL"
                     /locus_tag="IXF54_000533"
     CDS             complement(49270..49875)
                     /gene="dhaL"
                     /locus_tag="IXF54_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1403818.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49903..50886)
                     /gene="dhaK"
                     /locus_tag="IXF54_000534"
     CDS             complement(49903..50886)
                     /gene="dhaK"
                     /locus_tag="IXF54_000534"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1403819.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(50888..51283)
                     /gene="dhaM"
                     /locus_tag="IXF54_000535"
     CDS             complement(50888..51283)
                     /gene="dhaM"
                     /locus_tag="IXF54_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1403820.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51437..51757
                     /locus_tag="IXF54_000536"
     CDS             51437..51757
                     /locus_tag="IXF54_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1403821.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51869..52738)
                     /locus_tag="IXF54_000537"
     CDS             complement(51869..52738)
                     /locus_tag="IXF54_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1403822.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52834..54138)
                     /locus_tag="IXF54_000538"
     CDS             complement(52834..54138)
                     /locus_tag="IXF54_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1403823.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(54135..54644)
                     /locus_tag="IXF54_000539"
     CDS             complement(54135..54644)
                     /locus_tag="IXF54_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403824.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(54819..56954)
                     /locus_tag="IXF54_000540"
     CDS             complement(54819..56954)
                     /locus_tag="IXF54_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1403825.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(57037..58692)
                     /locus_tag="IXF54_000541"
     CDS             complement(57037..58692)
                     /locus_tag="IXF54_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1403826.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(58694..59008)
                     /locus_tag="IXF54_000542"
     CDS             complement(58694..59008)
                     /locus_tag="IXF54_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1403827.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            59178..59438
                     /locus_tag="IXF54_000543"
     CDS             59178..59438
                     /locus_tag="IXF54_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1403828.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            59452..59766
                     /locus_tag="IXF54_000544"
     CDS             59452..59766
                     /locus_tag="IXF54_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1403829.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        17784 a        12355 c        10672 g        19072 t
ORIGIN      
        1 aaaaggggat gtgacactgt cacactccct ttttaattta ttctatttct ttatatttat
       61 tttgtattcc gttacaaact cgcgtacttg gttaggttcc agagtgacat ctccaaaact
      121 ttcaagtcgc tccgctcctg gcagttcttg agtctcaaat gtgataccgc catgatttac
      181 taatttattc ccgcgcattt ccggtccatc ttctccaaaa tttgctgtaa aaatcacgat
      241 tcctgaagca tctgttttta cttggatcga tactttttca tctgggcttg tcaattccgc
      301 agcatttagt aaaccagatt gttttttcaa caaaaatgga tgatcgatgc cgcccactat
      361 cattgattgg cgaaaatcac tggcaaagac ctcacctaat tttttaggac gttggaagtc
      421 aaatgctgtg ccacttactg aaactacttc tcctgtaggc gttgtatctt tattgagcgt
      481 agcaaataat cggctattca accaaagtat atgttcgtcg attgactgtg ctggatcacc
      541 ggttaaattg aaatagacat gatttgtagg attgaatact gttttgcggt cactgatccc
      601 ttggaccgtc actcgccatg tatcatcgtt cgtcagcgta taccgtactt ctacatctaa
      661 agttccagga aatccgtttt ccatatctgg gctagtctga tagaaaatga ccgatgcttc
      721 gttttcacca ttgatgactg tataatgcca ctgcttggct tcaaagctag gtgcaccacc
      781 atgtaaacaa tgaccagtgt ccgggtctgt ttttacttcg aacgtttcat catctaattt
      841 gaatgtgcca ttcgtaatcc gtccagccat acgtccgatt gaagcaccga tataagggtc
      901 tttttctaga tattcttttg cttcatcaaa acctaagatc aactctcgtt tttcattatc
      961 gattattgtt tgaaaatgaa caacccgtgc cccaagatca ctgacagaca aagatgtccc
     1021 attttgattc gtgatcgtaa tcaagtgata gttttcgcca aaattttgtt cattgatttc
     1081 catgttcctt gcaccctctt ttctttcatt tgcactgatt ccagtataca tggaaaccgt
     1141 tttcttaaca gcttattccg tgagtaacga gaaaaaattt actatattct aataaaagac
     1201 cgtgaaaaaa aggttgtgac aaaaatattg tcacaacctc tttttccgaa caaacggtgt
     1261 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag gagctatttt
     1321 tgcttggctg ttcttattac ttgatgcagg acagcttttt cacaatctct ttttcaatac
     1381 gattccagta tataaccatg tcgtcagtac atagtagatg aaatacaata cgaagaatat
     1441 cgtaaacgga acccaaatat ctttgtatgg attttgcatg aatagaccaa acatttgtaa
     1501 tccgaataag acatggacta cacctaagat tcctggtgct aagaataaca cgcctacttc
     1561 tctacggatc gattgtttaa gcaacccgcg tcgtgttcct atcttttcaa gcatttcata
     1621 acgagcaatg tcactcttgc ttcctgacaa gatcttgaac atcaaacagc tagcaagcat
     1681 ggtcaagaaa gctaaaccta ggaagaatcc cataaattca aaaccagaga atagaccatt
     1741 atacacttca tacatttcat atttctggtt gctgtactct actttttgca aagatggatt
     1801 gttcatttta ttttgagcaa caagtgtttc gattggtttc aggtttgctt ggaagtcttt
     1861 tacctgaact agctgtaaag tggattgtgg caagttcagt tgatcaaatt ctgattgact
     1921 gactaccttg atttcttttt cttgctgttc cggcaattca tagcttcgaa gttcctcttg
     1981 tacagtcagt tcattcattt ttttagcagg aactttttct ttcgtgatct tcggcggtgt
     2041 tccatcttca tgttttatca tgatcaatgg ttgagccgca aattcacttg cattatagta
     2101 aatcgttccc gcatcttctt tttgagtata agaagcagaa agtgttggtg aaagttcttt
     2161 gattttttgc tgatcttctg cccgaggatt attcatcact agatcatatc cagaattgtt
     2221 ttctgtcaag tctgcaattt catttttaaa acctaatcct accgtcaaag cgcccagtgc
     2281 caaggcaaac agcatagcga ccattgagag catttgtgta tattcacgaa tacgaaagtt
     2341 taactgtgac aatgtgaaat tgttcaattt tttcaatgaa atctgttctg atttctttag
     2401 taatgataaa aagaaaatga tcacagaatg gaaaatgaaa taagttccta atacgatcgt
     2461 aactaaggct acccctatac caaagacttg ataggtcccc acgtgtttca tcatatagta
     2521 accgcagcct agaaaagcaa ttcccagtac tacttcaagg aaaaacaaga atttatgctg
     2581 cttgattctt gttggtgtct gatccgcacg aatcaatgct aaaatcggtt ttttgacaat
     2641 cgaaaaagca ttgacaacag cagctaataa aaacaaaaca gcaaaaaaca aaacagtgat
     2701 caacaacccg tgaatactaa aaggtgtaaa atgcgaaata ttgagatcta actgatccac
     2761 taataatcgg tggacgatac ttgtcaatcc tacaccgaca gctgaaccca ctaaagtagc
     2821 tgcgatccct actaagaatg tttcagaaaa aatcatctgt gcgatttttc tgccttttgc
     2881 tcccagcatc ataaacattg catagtcttt ttgccgcatc gtcatcaaaa aggaatttgc
     2941 atataaaata taaacgaatg tgatgatccc tagtaaaaca gaaccgaatc ggaaaatgat
     3001 aactgtagcc gcaacgatcg tattactttt caaaaaagct tcgttgcttg ccattgattc
     3061 aaacatgtaa aaaatagcag atgccataac taatcctgag aataacacca agtagtctct
     3121 taaacggctc ttgatcccag taagtgataa tttccaaagc atagtcaatc tccccctact
     3181 gttccaagtt tccaagaata gatagtattt ctcgatagaa ggaatcacgg ctttgatctc
     3241 cacgtttcac ttcttgatgg ataccgccat ccttgatgaa taaaatacgt tggcaataac
     3301 ttgcagaaaa cggatcatgc gtcacaagaa gaatcgatac atgatctttt gtattcaatt
     3361 cgtccatcgt atctaaaaga tcccgggcac ttttggaatc cagcgcacct gtaggctcat
     3421 cccctaatag aatcgttggt tgtgtgatca atgcacgtgc ggcggcgacc cgttgttttt
     3481 gtccaccgga aatctcagaa ggatagcttt ccaaaatatg agagatggat aaacgttctg
     3541 caattttttg gacacgttgc ttgatttctt ttggtctgac gccttgtaaa gaaagaggaa
     3601 cagcgatatt ttcttgtgct gtcaagcttt ctaataaatt gaaatcttgg aaaataaagc
     3661 caatctcatt cgcacgaaaa tctgctagtt gatttccttt taatgtggtc acatcttttt
     3721 gattgattcg gatatgccca tctgttggtt tatctaatgt ggataaaata ttcaaaagtg
     3781 tcgacttacc agaaccagaa gcgcccatga tcccaataaa ttcgcctttt tctacatcga
     3841 agctgatgcc gcttaatgct tgcgtctttt tctcattgtt ttttccgtat acttttgtta
     3901 cattctgtac ttcgactatt ttgttcattt ctttccctac cctttaatta gtttttccag
     3961 tgctgttttc ggcacgtctt ttttttcgac ttcttcatac gaagtgacac ctttattttt
     4021 gttccaggtg atttttaaat atgctccttt acgaagcggt cgagtcaatt gtcccttgaa
     4081 gtccagttct ttttccttac cctctttcgt atagccaggc aggatatatc tatagttgac
     4141 tgtttcttgt ccagagtctg attttgaaac tgtttttgta ccatctgtcg taatttgtac
     4201 atagtattct tctccaccca taactacgta atctgcgatt ttcacccctg caagtaccac
     4261 aacgatgaaa acaagtagac cgatcagaag ttttttcact ttttcccctt ctctcttact
     4321 ttatatactg attatagaaa aaagcgaaca attctgcctt cgaatcaact atcattcttc
     4381 tttcaatttt gtcattttct ttaaaacaac taaaaaaaga gttcaagaca ataatccgtc
     4441 ctaaactcct tcttcctatc ttacaaaaag tatgtcactt acggtctttc atgtcgcctg
     4501 tattgataac aggttgcgtt cctttgtctg taatgattga gacaagtaaa ttattgatca
     4561 attggacttt ccgatcatcg gtgaaattga tttcttgtcc atcttcgatt tgctctaagg
     4621 ccatttgagt gattgtgaca gctccttcaa caatggtttg tcgagcagac aaaatggctt
     4681 ttgcttgttg acgttggagc atggcactag cgatttctgt tgcataggct aaatggttca
     4741 agcgtgtttc gattacttcg acacctgcta ctgctaaacg ctcttgcagc tctttttgca
     4801 actcgtcaga cactgccgtc gtgtttcctc gaagcgtcaa atcattctca ttgaatgtat
     4861 catacgggta ttggctagcg atatgccgaa tggctgtctc actttggatt tctacaaaat
     4921 cttgataata cgctacatcg aataatgcct ttgctgtatc aacgacttta aagactacta
     4981 ctgctgagat ttcgatgggg tttccatcca aatcattgac ttttaaaaca gaactattga
     5041 aattccgaac tttcaacgaa acagtcattt tttgtgccaa aggtatcgta aggaaaaagc
     5101 cattttctcg aatcgtacct aaatattgtc caaaaaacaa aattactttt gcttgattag
     5161 gactgactac agttgccgaa cttaacaaca ataacgtgat caaccaaaga aaaatactta
     5221 aaaaaagtgc ccaaactttc gcttgccaca tacctaaata gaagaaaaac aatccaacta
     5281 aagctaatac gaccaatccg acaatccctg cataaccatt gatataaaat gctctttttt
     5341 ctctcatgct acttcctgcg ctcctttatc cttcaataaa tatctaatgt tactttttta
     5401 attttttttt cgaatacgcg ccattacacc ttctagttct aactctttgt cttcagtgat
     5461 gtaccaaccg cttgcctgtg tccctccggc tgcctgcggt aaataatcca attttgttcg
     5521 aacccagtgc ttttcttctg taaaatagtc ttctaccaag acctctagat ccatccctgc
     5581 tgaaagaggc aaatcttccc gaacagattt aaagataaat cgttccttct tcccatcata
     5641 cttcaatatt cctgtatcga taatcggtaa agaataataa tccagctctt tcttcaccgt
     5701 caataaatgt ttgaaaactt catggtacac ttgctctaat tggcgacctt ctgaattgtt
     5761 cgggtaatta accatcactt tttctttgtc tggatcatag aaggtttggc ttagttgagt
     5821 gatataagta tgtaatcttt ccatttctgt ttttaacttt tctctttgac cttcttctac
     5881 cttcatatac tctttcaccc tgttttcttt ttagatattt tctttcttat tttactatag
     5941 ttatgtgcgt taagcgatga aaaacagcaa agattggaag gatctttgct gttttttctt
     6001 aaaatatcca gttgagtaca ggatcaatct gtttcattgt cctcttgatc cgtcgtaata
     6061 ttgatcgatg tgtctttagc agtgatcttt agctttttct gtccttgttc taacaaagta
     6121 tttgttattg gctggtagtc tttttcaata taagattcac cagacgtttg gatagtatag
     6181 ccactgtttt ccatctcatg caaagataaa ttgccaccgt ttaggatgac ttttccttct
     6241 ttgctgatca aactgtcttc tagatggaag ttgctgtttt cacctgtgac gatcaagtta
     6301 tccacattgg cctcatctaa tcgaagtgag cctaattcat ctttcgcagt tattgttcct
     6361 cctgtaaatt cattgaacga tacccagcca tcatgtgtct tagtattcac ttttttgatt
     6421 gatacttcgt tgatatcaat tgcactatta ttcccttcga ttgtcaattc agataattca
     6481 tgatctgcag ggacaacaat agtgatttgt gcacttgtgt cttctaaact aatgattgca
     6541 cctgttgatt ttttgactgc attcacagtc aacacgccat ttttctcctt tatttctggc
     6601 ttttctgtct tatcataagt cgtttttatc tcaaaatcca atccgcgtat gaccgaaact
     6661 gtttgatttt ctgcatggat gacgattttg tttgcatcct tgattttttt tgtctcagta
     6721 accatatcga tcaaccgagg accattatcc caagtcagac tcttttgagc gcctaatgta
     6781 taagttgcac ctgctaggag gccaccagta agcatcagac caataccggt aatcaaaaaa
     6841 tgtttcaatc tcaatttatg tgtcctcctt caaacgcccg ctgaatgcag atctcttttt
     6901 gtaataaaac gtcgtcccat ccatttaaca aagttcatca tgacataaaa aagccctttt
     6961 gttcctttca gcaacagtgg cgaaataaat actgcgcttc ctgtggcgaa cagaccgaca
     7021 ccgataaaga ataatccact cagaatcgat tgtccaaaca cactgattcc tgagatgatc
     7081 atgaataggc caacacctaa gatactgata cataaaacat agatactaaa aatgagcgct
     7141 agtaaagcaa ccaatagtcc cccaataacc aatataaacc ccagtgctag tggaatgagg
     7201 acaggagaag caaataaacc gataatgatt aaccaaatca ggcgaatttg tctttttgga
     7261 tttttcacta actcgttttc tgcttgttct tcttctgaaa agtaactgat ttttaatttt
     7321 aaggcaaact gctttggtgt accatattca tcaatgatct gatctaatgt ttttccagaa
     7381 tctaaaaaat attcctcata atactggatc aaatcaaaac gatcgtcttc aggaatttct
     7441 tgaagatgat ccacaacttc attcaaatat tcattcattt gtgctctcct ccaataaccg
     7501 gtctaaagaa tctcgaaaaa caatccattc atcaataatt tcttctaaat gacgacttcc
     7561 cattggtgtc aacgagtaat atcgacggtt tctgccttca aaaggttcat cataggtctc
     7621 aagatatcct tcttttttca atcttctcaa aaccggatat agtgtagatt cactgacaga
     7681 gagactttct tgtacgattt ttgttaaggc atacccatac atatcttcct tgcttaatgc
     7741 tgctagcaca gtaccatcaa gaagaacagt tggaatctgg atgtgcatta cgtcaccttc
     7801 tattctcaag tgtaatattt tatttcaatc tatattatac aacatataat atagtttcaa
     7861 aatcagtttg tcaaatacaa aaaaactgac taaaagattt gatccttttt agtcagcaaa
     7921 aaatggcttc tctttgattt ttattataac agtggcataa tccaatttgt ccacatccag
     7981 ctcagtggca taataacgat cattgcaggt atcatgtcag ctactgggaa cattttcacg
     8041 ttgatcattc taaaaccggt agcgaccatc aagaatccgc cgcaagcttt aaaatctaag
     8101 atcatgtctg gtgtagtcaa tggataaata aattttgcca ataagaacaa aatataaaaa
     8161 ataatgaatt ggggaattgc tatcacagaa acgacatacc ctaaattaca cgcaaaaata
     8221 gctgctgtga aaaaatcaag gacagattta gaaatcaaaa tcgtactgtc tcctgtcatc
     8281 ccagaatcta aacttccata gatccccgta ccacttgcac aaaatagaac aatgaccgtc
     8341 actaatgtgc tgataaattc atcgtgggtc ataccaagct tttcactagg gaagatttta
     8401 gaaataggta cttgcatcag cgcagccccc ttatttatcc cattacctaa atgaacaatc
     8461 aaaccaatcg ctgttcctat cactaaagca aaaataacag ccggcatatt tttcatcggc
     8521 gcaatggaat agattcccat ccccatcgaa cacacaccaa aaaccatcgt cagctgtgct
     8581 ttaaaatgtt cacttaattt atttcctact aatccgccaa ttagtccgcc aaacaaaacg
     8641 gaacctacat tgatcaaaac tccagttggc atcaaaaaac tcctctcttt ttctacgaag
     8701 cgaaaaaaat cagaaaaatt tctgtaatta ttgctttcgc tgcaagaaag attgtaacag
     8761 acagagaaga ttacaccaat tcattttttg tgttaaagta ttctttattg gaataagtag
     8821 gaggaatatg atggatatcc ataaattcga agtttttctt gatttagccg aaacaatgaa
     8881 tttcaccaaa actgctgatc gacaattcac cacccaagga aatatttcta aacagattct
     8941 ttcgttagaa acagaattag acgtcaaatt attcgaacgc gcccatagaa aaatcgaatt
     9001 gaccgaagct ggaagcttat tgctcccata tgtaaaaaat gtcgtagaac aataccatgc
     9061 tatgcaagaa gtgcttgaaa cctatacgaa agacaacaat ctttcattaa atatcttaac
     9121 gataccgaca atgatcaatt ataaaggttt ttccaaaatc accgagtttt tgaaacgcca
     9181 tcctgaattt acagtccagt tgaaagaagt ggaaagtgtc gaattaactt acaatgacgc
     9241 caatgaacat agtgacacga tccactttgc tagaagtttc caggccccac caagttcgat
     9301 tgagtggttg cctactgaaa cagacgattt tgtagcagtt ctacctaaaa atcatccttt
     9361 agctagtaaa aagaaattgg atctaagcga actgaaaaag gagaactttt tgctccttgg
     9421 ccccactacg aacttgtatc aacctgtact agatctgtgt cgcgaagctg gatttgaacc
     9481 aaaaatctct tataaaggag caagaatcga cttgatcatc aatatgattg caaatgacat
     9541 ggggattgct attgttatgg aaaaaacagt caagaatcta ttaaaagaaa atactgtgat
     9601 tctccctatt tctccaacaa cagagagtta tcttgcattt actcgtaaag tcggtgagca
     9661 ttctttggct tcagatacat tttgggagta tttaaatcaa gaagaaaact aagccctaca
     9721 ttttctgtcc atctatccac ccaaaaaaag agagcgacgc atgactttcg ctctctttct
     9781 tgcatcatgt ccttgtttca atgacacctc taacatttct cgtagttcaa taagaaagca
     9841 gcgattttta tgccaaaaga accgtaagaa catttcccta atcacaaaaa aacgactaaa
     9901 aacccctaaa aaccttcaat agaattttta ggggttttat tctttttcct attgtttact
     9961 taaccgtatc attgctgtcg ttcctactcc ttcgatactg ctataatcaa tacgtccatc
    10021 atgcagttct agaatttttt taaccagtga aagtcctagc ccattcccag aaatttgacg
    10081 acttgtatct ccttgataaa atttttcaaa cattcgatct ctggtctctt cattcattcc
    10141 tacacctgaa tcggtcactt ccagtacaat ctctgtggct gtctcaaata gtttgatatg
    10201 aatctgtcca ttgatttgat tgtactttat ggcgttatcc atgatattca gccacacttg
    10261 gtacaaaaac tcttcattgc ctgtgtaatt tactgcagca agttcgatat ccaattctag
    10321 ctgttctttc tcccatttag gctgaaaaaa aacgattact tctctgattt gttcatctaa
    10381 tcgatactct ttcttttcca gttggatacg ctggttttcc agtttgttca attttaagat
    10441 gttctctgtt aattgtgaaa gctgcgtgat actttcgata atccgatata aaaatatttg
    10501 gcgttcttcg tcagagagat ttggggcttg taacaattga acatatcctt ggattgttgc
    10561 tagtggtgtt ttgaattcat gggatacact ggacacaaag tcattcctca gtgtctcgat
    10621 actattgagt tcttggacca taacgttgaa atctttatac agttgctgta cttctgctac
    10681 tttctgctgt tcatccattc gaatcgaaaa atctcccgtt gctaccagac tcatatttgt
    10741 ccctagcgca ctgattggat gcaggatacg ccgcccaaca aaaatcgata ttcctgtacc
    10801 cacgaccata ctgaaccccg caaagacgaa taacgggtat aacgttggtt ttccagccga
    10861 ttcagcaggc gagatagaat gtcgagacaa taagaaaata aaaccgagaa aacacagcag
    10921 tgtcactaag atcgaaatga agacaatact agcaaaatag atccataatt gtgaaaatag
    10981 cttttttttc atctttaaat aaccgcctta tagcctagtc cccgtactgt gatgatttga
    11041 aaatcttccg tcttttcaaa acgggtcctt aaacgtttga tatgtacatc gatcgttcgt
    11101 tcttctgtat tcgtatctaa tccccaaata tcatccatca actgttggcg agtgaagatc
    11161 ttgttgggat aagacagtaa tttatacaga agcagaaatt ctttttgagg cagaatcaac
    11221 gaatgctgat cttgtaacac tgtataagca tctagctcta attttgtttg tccgattatc
    11281 aagctactgc tgtaattgat ctttgaacgt cgaagcaatg cttccacacg taggatcatc
    11341 tcattgacat cgatgggttt tgtcatataa tcatctgctc ctaatttgaa cccgcgtttc
    11401 ttatcctcaa aatcggcctt tgccgtaatc attagaacag gtgtgtcaaa tctggtgcta
    11461 cgtaatgagt ctagcaagtc atagccatcc attttcggca tcatgatatc tgtaatgatt
    11521 aattctatac gtgtcgcttc tagttttttc agcgcatcct ctccattcaa tgcggtgatg
    11581 actgaaaagc ccgctcgttc caacacagaa cggtaaagca actgtaattg cggatcatct
    11641 tctactacta aaatacgatt cattgatctc cctctgtttt ttgaaaaatt tctagatttt
    11701 ttcttgtagt tcatcttcag ttaacattta tttcgtatac tacgatcatt gttctgatat
    11761 acttattagt ataccgaata tttcaatacg ggagaagggt gattatgctt caattaaaag
    11821 atataaaaaa atattataaa gtcggcgaaa ccaccaccaa agcattggat ggtgtgtctg
    11881 tcgctttcag acaaaaagaa ttcgtagcga ttttaggtcc tagtggttct ggtaaaacga
    11941 ctatgctgaa tgtgattggt ggactagata attatgattc cggtgatatg gtaatcaacg
    12001 gaaagtctac taaagacttt aaggacagtg actgggatgc ctaccgaaac aactctatcg
    12061 gtttcatttt tcaaagttac aacttgattg gtcatctagg cattattgaa aacgttgaac
    12121 ttgggatgac attaagtggt gtttctaaag acgaaaaacg aaaacgggca gaagatgcat
    12181 tgcaccgagt cggcttgaca aatcatatgc ataaaaagcc aaatcagtta tctggtggac
    12241 aaatgcagcg agtggccatc gctcgagcac tagccaatga cccagatatc cttttgtgcg
    12301 atgaaccaac cggagcgtta gatacagaaa caagtatcca gatcatggaa ttgatccaag
    12361 agttatcgaa agaaaaatta gtgatcatgg tcacacataa tcctgaactg gcccatcaat
    12421 acgcagatcg aatcattgaa ttttctgatg gtaagatctt aacagattcc catcctcaca
    12481 tcgaacgacc aaaagatgat caatttaact tacgcagaac aaagatgagt ttttggacag
    12541 cgttaaagct ttccttcaac aatatccgaa caaaaaaagg acgtactttc ttgacctcct
    12601 ttgcttccag tattgggatc atcggaatcg cgatcgttct gtccctttct tctggattcc
    12661 aaaaacaaat cgataacacg caagctgaaa cgatggcgaa atttccgatt actatttcta
    12721 aggtaacgac taaccagact cgtgacgatg ccggattggg tgcaagcaaa gcggattatc
    12781 cagacagcaa aacgattact gcaaaagtca gcgaagaaga taaagcacag cacacgaata
    12841 agattgatca aacatacgtc gattacgtaa cagacatcga tcctaactta agtaataata
    12901 tcggatttac ccgtactaca ggaatcaatc ttttacgtga tgtaaatggc aaagtacagc
    12961 ctgtcagctt ctcgaatcaa aatcctgata cggaatctct ttctttatct agtacgatgt
    13021 cagcaatgac aggtgtggga gtgtcaagtt tccctacaca gcttgacact agcaaagaaa
    13081 actttttaaa agacaattat tctttacttg ccggaagtta tccagcctca gcaacagatg
    13141 tcgtgctgat cgtagacggc aataataata ctaatatcaa cgcattgaag aatcttggat
    13201 ttgatgtgaa agaagacgaa aaacttgatt ttgatgatat cgttggtaca acattcaaat
    13261 tagtaaacaa caatacttac tatacaaaac ttccaactgg gaattttatc cctaatacag
    13321 attatgatgc gatgtatcag aatgcttctg atgaattgaa gatctcgggg atcctacgcg
    13381 tgaaatcttc ttctacaatg aatctcttat ctccaggaat cgcttatagc gatcaattaa
    13441 cgactcaaat cgtcaacgaa aacaaagaat cagaaatcgt caaagctcaa agagacagtg
    13501 gtgtaaatgt cttaactact gaaaaagttg acgaaaatgc caaacaaaca ttgcttagtt
    13561 atttaggtgg agacagcttg ccttcaagta tcatgattta tccgaacaat tttgaagaca
    13621 aagaaaagat cttggattat ctagatgatt acaataaagg caaatcagat gaagataaaa
    13681 tcatttatac tgatttagct ggaacgatga ctgaactgac tggcggattg atggatgcta
    13741 tcacttatgt ccttattgct tttgcaggga tctctttagt aacaagtatg atcatgatca
    13801 gtatcattac ttatacatct gtgattgaac ggacaaaaga aatcggtgta ctaaaagctt
    13861 taggtgcacg taaaaaagac atcactcgcg tatttgacgc agaaacgtgt attttaggta
    13921 tttcttctgg gatattaggt gtgtttattg catggctagc tacgttccct atcaatagca
    13981 tcttatacaa catgacggat ctaaaaaatg ttgctcaact aaatcctgtc catgcgatta
    14041 ttctagtgat cgtctctact atattgacga tgcttggcgg tcatcttcct gcacgaatgg
    14101 cagcgaaaaa agacgccgct atcgctttgc gtgcagaata aggttgtgac aaccacattt
    14161 agatttgagt cataagagcg ctgaaatagt agaaaacagc aagaaacgag gaagtaatat
    14221 gttccttgtt tcgcgctgtt ttttttaatt attttttata attctggtat acttttcttt
    14281 cctttgtacc acaactcttc tggcgtatac ctgtcttttc catttatgaa tactgttcca
    14341 tctgcttcaa tcgttacttc agtcccaatc cacatattac gagaaagtgc gagaagattt
    14401 tttctttttc ctcttccttt ttttccaaat tgcaaggcat cggttatctc ttctgtccaa
    14461 tgagatttca cgtattttac ttctccgttt tcaaatgtaa ctttcaaaat attcgctata
    14521 ttttccactt ttactgcttt aggaaatgac tcctccattt ttttcacttt ccttcctcca
    14581 acactaagta tgcgctttct tttagtgata agtatatcat agtttattct attatcaaaa
    14641 aaacgcatcc attccattgt agaatacttt accgaacaag ttgaattgtt ttcctctttt
    14701 tcacctttta ctatagatag gtaaagacaa ctaaggagga atctctcatg acagatcaac
    14761 cgtatgattt aagaaaagtc atcgaagaac taaaaacact tccaggtcaa tatcacgaaa
    14821 cagatattga aatcgatcct gaagcagaat tatctggtgt ttaccgttat atcggtgctg
    14881 gcggtacagt aaaacgtccg actcaagaag gaccagctat gactttcaac aatatcaaag
    14941 gtttccccgg cacacgtgtg aacatcggaa caatggctag tcgtaaacgt gtaggtgaaa
    15001 ttttgcacca tgattatcga acacttggtc gtttcttgaa agacgctgta gaaaatccaa
    15061 tcaaacctgt gaaagtagac aaagcagacg ctccagcaca agaagtcgtt catctagcta
    15121 ccgatgcaga cttcgatatt cgcaacattt tgcctgcacc tacaaatact cctgaagatg
    15181 ctggtccata catcacaatg ggtgtagtct taggatcaga tccagaaaaa acaatgagcg
    15241 atgtaacgat ccatcgtatg gttctagaag ataaagatac gatcggtatg tacatcatgc
    15301 ctggcggacg ccacattggt cacttccaaa aacaattcga agccttgaat aaaccaatgc
    15361 ctatcactat caatattggt ttagatccag caattgccat tggcacgact ttcgaaccac
    15421 ctaccactcc acttggctac aatgaactat ggtgtgcagg agctttgcgt aacgaaccta
    15481 ttcaaatcgt tgatggtata gcagtcgatg aagtgggtat tgctcgtgct gaattcatca
    15541 ttgaagcaga aatcatgcca aatgaaacaa tgcaagaaga tatcaacacg aactcaggaa
    15601 aagcaatgcc agaatttcct ggctatactg gggatgctaa tccagctgta caagtagtga
    15661 aagtcaaagc agttactcac cgaaaagaca atccaatcat gcaaacaacg attggtccca
    15721 gtgaagaaca cgtatctatg gccggtattc ctacagaagc aagtattctt gccttggtcg
    15781 ataaagctat tccaggaaaa gtagtaaatg tctacaatcc tcctgcaggt ggtggtaaat
    15841 tgatgaccat catgcaaatc cgtaaagaaa acgaagcgga tgaaggtatt cagcgtcaag
    15901 cagctattct tgctttatca gccttcaaag aattgaagac agtcatttta gtcgatgaag
    15961 atgttgatat tttcgacatg aacgatgtag tttggacttt caacactcgt ttccaaggag
    16021 ataaagatat catcgtgatc ccaggaatga ggaaccatcc attggaccca tcagaattac
    16081 cagaatataa tcctggctct attcgtttcc gtgggataag tgcaaaaact atctttgacg
    16141 gtacagttcc tttcgatatg aaagaacatt ttgtacgtgc aaaattccaa gaagtagaag
    16201 attgggaaaa atatttgaaa taatgaatga tctgcaactg ttttgacatc ataaaaagga
    16261 gtgcggaaaa tggaacaatt ggcacaacgg aagaaaaaaa tcgtgattgg tgtaagtggc
    16321 gcttcaggca ccatttatgc catcaaccta gtgaagaaac taaaagaata tccgatgata
    16381 gaaacacacg gaatcatcag tccatgggca aaacagaact taaagctgga atccgactta
    16441 tcactaactg aattcgaaag cctgtttgat taccattatt caaacaaaga tttaggtgct
    16501 gctgtcgcca gcggatcatt tttgaccgat ggaatgatca tcgtaccggc aagtatgaaa
    16561 acagtcgcag gtatctctgt tggtttaggc gacaacctga ttgcacgtgc tgctgatgta
    16621 acaatgaagg agcaacgaaa gttagtcatt gttcctcgtg aaacgccatt aaatgccatt
    16681 cacttggaaa acttgactaa attagcacga ctaggtgtgc aaatcatacc accaattcct
    16741 gctttttata atcagcctca aacgatccaa gatcttgtta atcaccatac aatgaaactt
    16801 ttggatcaat ttgaaattgt aaatgagcat tcaaaacgtt ggcagggtga ttaatatgga
    16861 aaaagaaacg atagttgaag taaccaaact aggtttcacg atccaagctg ctgttaaagt
    16921 aatcggtaaa gacttattga tcactttaac aggcggagat acaccacata tcggaacagt
    16981 cactacattt tctcgtgata cagagatgca aactgtacgt ttccctagcc acgatggtcg
    17041 gttccataaa gacgatgtct tagccgaaaa aatagctgtg atcatcaaaa ctgccgttcc
    17101 gggaagttgt acgatcactt ccggtatcca tgttgaccat atctccaatg cacaaatcaa
    17161 cgcctctttt ccgatgtcag aagagttagg ttacaagatt ttagattggt tggaaaaaat
    17221 agatttccat atcaaagaac cagtttatta taaagatggt gaaaaaccat tatgaaagaa
    17281 gaggttgtga aatcagcgtt atgacctgag tatgagagaa aaaaatggaa aaatagcttc
    17341 tcatattttg ccgtttttga gctaataccg agggacagct gattgaacgc cgtttatcaa
    17401 gaggttgtga taaagccgct tcgtttcgag tagtaagaaa aattttgtaa acatgggttt
    17461 ccacattttt gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc
    17521 aagaggttgt gacacgtctt ttgtcacaac ctcttttatt tttgcttcac tgtaactgct
    17581 acaatatcac cgatttcttt tccgatgttt ttacgaatat cttttcgtag tccaaggatg
    17641 tagcagactg aaccatctgg atttttcacg cccatattca cgatactgcc ttcatacggc
    17701 acattgtcaa aagttgcttg gactttaact cttccagcac cgaattcttt tcttatatca
    17761 tacggaaaaa taatatatgc cccgcccttg ccgacttcgg aagcatagat ttttgattca
    17821 aaagaataaa tttccctcat tttatttctc ctatttttta catctagtat agcatagcaa
    17881 aaaaaagaag gaactgtgac aaaagtcttg tcgcagttcc ttttttcgaa taaacggtgt
    17941 tctcctgcca actcctcggt cacaagtcac aatagtcaac aaacatgaga agctgttttt
    18001 tgaaattgct ccttgtgact cagagttaaa cggaaggctc acaacctctt ccttaaacgg
    18061 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    18121 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tcctacatgg
    18181 tgttcaaaat attattcgcc ttttgcttct ttcactaatt ttgcgaatgc ttgttgttgg
    18241 aattcagcag ctaaggcttt cgtacgttgt tcttgctctg ctttgctcat ttttggattt
    18301 tcagtcattg cttttaccat accgtcttga acataagctt ttgcttcact ttccatcgta
    18361 ctttgctgtt tcttcactaa ttcagctaac ttggctgctt ggtccgtact taattctacc
    18421 catgtctctg ggattttgaa tgtattcact cggctgtcat attcgtgatt gttatcagca
    18481 atattgaacc agaatttata catgtcgttt ttgtattccc aataagtagt tttcgtcact
    18541 ttttcagcat cgccgtctgt cttttctact ttatttgttt cgttatccgt accagttgct
    18601 tttggttttt cttgtttttc atttgttcgg taaagataca ctttttcttt tccattccct
    18661 aaagctttat acaaaagcat atctgctcct tgggaatcag cactggaaac aattttttct
    18721 gtttttgttt ccgttacttt ttccattcca aagtgattgg acaaattagc gacgatcaaa
    18781 cttagtgaag cgacgaagat tagtccgaaa atcaaagata aaaaggtttg ccatgttttt
    18841 ttagcgaaca cgttcgttaa agcaaaagca agcaagctga caatcaaaac tccaataatc
    18901 atgcattatc ccccgctttc attagaacag gttctttttt cttcaataaa aaggcaataa
    18961 taaagccaag aatcccaaag atgatcgcta cgataaacgc ggcatggtaa cctgacagtg
    19021 tggcattgat tgcctgatct ttataagcta atggcgtatt atccaataca gatttgcttg
    19081 ggatatttgc ttttgtcaca ttcgtcaaca cactgatcaa aatagctgta ccaatcgagc
    19141 ttgcgatttg acggaatgta ttatttaccg ctgtcccatg actgatcaag ttatttggca
    19201 atgcattcat accagaagtc gttaccggca tcatgaccat ggagatccca aacatacgga
    19261 tcgcataaag gatcacgata tataaaactg gagtcgtttt tgtcaagaaa gcaaatggtg
    19321 cagttgccgc tgtcaaaatg aacatccctg tgatggctaa acgtttcgcg ccgtatttat
    19381 cgaaaatcgc acccgtcaca ggcatcatag cccccatgat caatgcaccc ggcaacaaca
    19441 tcaagccaga atggaatgct gattctccac gaatattttg aatatacaat ggtacgacca
    19501 tttctgcccc taccatcgcc atatttgtta caccagctaa aacagctgca atcgtgaatg
    19561 tagatgattt gaatacacga agttccaaga atggatgttc caaatgtaat tgacgccatg
    19621 agaacaaggc gatgaaaatc acaccaacaa ctaagaaacc aattactttc aagcttcccc
    19681 agccgtcatc tcctacacta gaaaatccgt aaagtaaact accgaatcca atcgttgata
    19741 aaatagcgga taaaatatca atgctaggat ttgacaatgg aataacactt ttcattagga
    19801 agaatgctaa aaccaagaca agaacaacga ttggcagtac cataccaaac aagtcacgcc
    19861 aatggtagtt atcaatgata taaccagata atgtagggcc taaagcagga gcaagtccaa
    19921 taacgatccc tgacatcccc attgctgatc ctcttttttc tggcgggaaa atagatagca
    19981 tgatattttg caataatggc atcgatactc ccacaccgca agcttggatc aaacgaccga
    20041 ttagtaatgt actaaaatta ggtgctgtaa agcaagtaat cgttccgatc aaaaagatcg
    20101 tcatggcaga aagatataaa tttcttgaac tgaatttatt gatcagccat gctgttattg
    20161 ggatcatgat cccgttgact agcaagaaac cagtcgttaa ccattgcaca tcagacgctg
    20221 aaatatcgaa agctttcatc aatgtaggaa aagctgtcgt caataaggtc tgattcaaaa
    20281 cggtacaaaa tgtaccaatt aataagacaa caactaaaag tgtccgatta tatggttttc
    20341 cgtaaatatc tacgggttgg ttgttactca tatttctcct tctttctgaa ttttttctga
    20401 aactactaga atactttagt cctaaattcg ctatttgtca acctacttga cacataagtt
    20461 aatacggata taattaaggg aagcaagagg acaaaaggag tgacaaaaat ggttggggct
    20521 tcccttaaac gtttgttgga aaatgatcaa ttggttgttg gcatcagtga attaagtgat
    20581 atcgtaggtg tgtctccacg tcaattaagg tattgggaac aaaagggatt cattcgttcg
    20641 atcgaagctg atgcgaattg tcctagaaaa tatcgtttac ctacagtcat aaaagtagaa
    20701 ctgatcaaaa aatatttaga tgacggattt actttgtcaa aagctgcaga aaaggcagaa
    20761 atccgccaaa agaaaatgca ccatgtccgt aaagtttttt caaaagcaat caaagatatc
    20821 gaattgatcg acgaacgcta tacgatttta caaatcggta acttcaaaga agagaatgct
    20881 aagatgtaca tcattcatga caacgagaca gacgaattaa cttacaagct ccttcctgtc
    20941 gatgaaaaag ctgactacga acagttgtta aaagaactct aaacgccact aatctcctaa
    21001 gtaccaagct ttttaataag tagatgctac ataaatactt gaaaaaaggc ctgtttgaac
    21061 ataagaatat ccgaacggtg atctgcgttt ctttcatata gaaaggaatt ctccatcact
    21121 gttcggatat ttttttattt ttttcggtta ctttcttcta atacatctgt tagccaattt
    21181 gcatattctg tttcccgatt gatcgcatgc gctaaaatca ggtagtgtcc gtaattttca
    21241 tgtttttctt tttcagtagg aaagatcgtt ttttttcgct ctaatagatg aagtcgtttt
    21301 tcttcatgaa ggtcacgctg ctgctgaata atttctacta atgccggatc attcacgtct
    21361 ttgacgaaat acaatttcag aacaaattcg tctcgattaa ccggtagttc attggtgggt
    21421 gtatgaatcc actctgccaa ttgttccttc ccttcttctg ttaattcata aactttcttt
    21481 tccaattttt cacctgtgat ctctacttga tgctgaatga ttccttgctc ttccattttt
    21541 gcaagttcag gataaatctg actatgtttt gcttgccaaa attctcctat ttcatttttg
    21601 aacgattgat tcaattcata tcctgtcatc ttcttcttac ttaataaccc taacaaaata
    21661 tatcgaagtg tattttttct tgccatcagt tttccctcct tgacaaatct ttaaatccat
    21721 tctatcataa atgagtaatc gaaaacatgt agtttattac atgttttaat ttataaggag
    21781 atgcttaaac atgaaagaat ttaaagaatt ggaagatttt attggaactc actttatcta
    21841 tacgtatgac aatggttggg aatatgaatg gtatgccaaa aatgaccata ctgttgatta
    21901 ccgtatccat ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag ctgatatcgt
    21961 taaattgacg gacggcgtat tcaaaatcac ttggacagag ccaacaggga cagacgtggc
    22021 attggacttt atgccaaatg aaaagaaact tcacggaatg atctttttcc ctaaatgggt
    22081 tgaagaacat ccagaaatca ctgttacttt ccaaaacgaa catatcgcag aaatggaagc
    22141 agctcgtgaa aaatatgaaa cttatccaaa attacttgtt cctgaatttg ctacgatcac
    22201 ttatatgggt gatgcaggta tagacaatga agatgttatt agcgaagaac catacgatgg
    22261 aatgccagat gatatccgta gcggaaaata cttcgatgaa aattataaac gagtaaaata
    22321 aggttgtgaa agagaggttg tgaaatcagc gttctgacct gagtattaga gaaagaaacg
    22381 gaaaaatagt tcctcatgtt ttgccgtttt ttgagctaat accgagggtc agctgattga
    22441 acaccgttta tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa gaacgctagt
    22501 tagaaaatag cttttcatat tttctaggct agtgggctta ttaccgagaa gcagccgttt
    22561 gaacaccgtt tatcaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    22621 atggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    22681 tgaacaccgt ttatcaaaga ttgtaacaac tgcatattca cacagttaaa ttagtgactt
    22741 caaaatgaaa taatgctccc agcaaaaaat taggctggga gcattatttc gttattctgt
    22801 tcatcaaaaa tcagcgtttt gattccattt catcacaagt tcctcctcat ttactgcttc
    22861 atctttttgc tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa tagctgattg
    22921 atttttttga tagactgtca aagtcagtgt tttattggtt tcttttattt ttttcattag
    22981 ttgcgtaccg attctttgtt gccggtattc ccttttcaca aagattcctg cgataaactg
    23041 atcaatcacc ccagcaaaac caataatttc ttgatctatt tctgctaagt aaatcactgc
    23101 atctggtaaa gctttcctaa cagattctac ttgattctcc caataagttg atggaatgaa
    23161 ttcatgagaa tccaagttag catgcagcca aatcgataga acagattcca gttctttggc
    23221 agacaatttt gttgttgtgc ggatcatttt ttctctcctt acttattcat tgattttaaa
    23281 ataaaaacca tttttttgaa gctcttttac aagttttcgc ccctcaaaaa agtatatctt
    23341 ctcttgtttt acttttctaa taccaaaaga aacagcaact ttaggatcat cagctgaaac
    23401 gattcgataa gttccaccag gcaattgatc cagcttgact ttcccatgat ggtcgctttg
    23461 tactgaaaac ggcacaccat ttctagtaag tgtttttttc ttatgtttca aaatgaatga
    23521 actaccattt accggtttcc ctgacgaatc actaataata aatgaaaaat caagcacttt
    23581 tttacttaat ctatactgat agattagtcg tccgataaac actaacagta tgactacagc
    23641 tattatcgtt cctgcaagaa taaaccattg ccgcatggtt ctttcctgat ttccttttcc
    23701 ttcttctttt tttaccgatt cgttataagg aatccgatgt cctgtcacta gcaaacgatg
    23761 cgtgttgatc atatacggcg tacatgtaag caaggtaact aagtcttctc cttctgaaat
    23821 cgttagaaca gacgtgtcat ctggcagtac cacttgtata tttttaactt gataagccaa
    23881 tttctccccc aaaacagtca aaataaacgt gtctccgttt tctaaacgat ctaattcggt
    23941 aaataaccgt cgattaggta aaccgctgtg agcagagatc actgaatgcg tcgaatttcc
    24001 accaagcggg aaagaggttc cttgcaagac gcctgctcca ttttcaagga tcctaccatt
    24061 gagccgatca taaagtgaaa tatttaattg gatcttaggt atattgatac ttccgatcag
    24121 ccattcctct aattggctgg atgttgtact ttcatttctg ttcttttcat tgaaaggatc
    24181 agtaccagga ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca ttttctttcg
    24241 ctgcacttca atttcagctg cggtccgttt ttgtacttgt tcgatacgtt tttgatcaat
    24301 aaaactattt aaagaatcca cataaaatgg atataaagcg atcagtaacc ctacgacgat
    24361 acatattaat aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct tactctgctt
    24421 cacatgtgtc ctcccttgca agtttttttg ttctttcttt tattatatgg gttcaggaaa
    24481 aaaatagaaa atgaaagccg atgaggttgt gaaaaagctg tcctgcatca agtaataagt
    24541 acagcccaaa aaaatagcta ctcatatttt ttgttgggtt gggcttatta ccgcagatgc
    24601 aagcttttga acaccgttta aggaagaggt tgtgaaaact gcgttatgct ttgagtaata
    24661 agagcgctag tcagaaaata gcttctcata ttttctaggc tagtgagctt attaccgaga
    24721 agcagctgat tgaacaccgt ttaccgagag gtcatgaaaa agctactttg tttcgagtat
    24781 taagaaaaat tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc atcctctctc
    24841 cttagaactt tcacttatgt ttttcttgta aaattcataa atattttcat gaattttcta
    24901 atttcttgcc tacctctcgc ataggatata tgatgaattt atctaaagtt tgcaaaggag
    24961 ttcgacaatt gaaaaaatat ggcccatttt tagcaatttt tttcattgct ttggcatttc
    25021 tcatgcctca tttattcaca cataaactaa ttttgggtgc tgatgcccca ttccattaca
    25081 atcggttcta tgatgccgcg caacaaatta aatatgggga tttccattat tttccttccc
    25141 tttatggatt ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca tttttcgctt
    25201 atttacaagg attgatcgtg ttgcttgcag gtagctggtt tcattatcag attctttcaa
    25261 atttcattct ttatcttatt tcaggatttt ctatgctgat tttactgaga tatgtcaatg
    25321 ttcgaaattg ggtaagtgta ccagtagccg ttttattcat gagtacgtat tctattcaat
    25381 actggtctat caaccaagga ttcactagct gggggacttg tttttatcct ttatgtatga
    25441 tccctattgc tgatttcgtc cttcataaaa agttccctgt tatcaaagtc gctgtctcag
    25501 tgggacttat gacacagatc catttactgt cttcgatcat gttagctttg atgtatatcc
    25561 ctttttatct ttattacttt tttaaccaga ctgaaaaagg aaaagcactg cttcaactga
    25621 ttaagtcggt cttgctttat tttgttctga cagcaaatat ttgggcagga ttagctattg
    25681 tttacagtgg gaataagatt cttcctccct ttgttaacag agaaatgagt caaaaggcga
    25741 tagatctgga tggcagttat tggctccatt accctaaggc ctttcctata ttgatattat
    25801 caggtttttt gctttttatt ttctttcaac aaaagaacac cttattccaa aaaatcgtgt
    25861 tgtacacaag tcttgttttc ttttttcttt ctactagctt gattccatgg acgacattga
    25921 tcgaaaaaaa tgtgcctttt attagtttga tccagtttcc atttcgattt ttcgtgccat
    25981 ttacgctatt gcttctactt taccttgccc tttcactgaa ccagtggtct gaacaaaagt
    26041 ggttccgtct tttaagtata tttggcattc ttattttttc agcacagacg atccagaact
    26101 tataccagca tctggaaaag tgggaaaatg aaacatttgt cagtcgacat acttatctat
    26161 tcgatacgcc ggaagaagca aggaaaacat tcttcagcaa agatatgtct accagcttgt
    26221 tcgttttcca aaaaaccaca cctgactatc tgccaatcta taaggagaca aaagacaata
    26281 aatacgaccg gtataacgaa gaaatactag aaaacgaaac aacttttata aaaacacatc
    26341 caggcggtct tttgacaatc aagtggaaaa acactgggga caaaactgtt catatccctg
    26401 ttatcgtcta cgatcggact gtgctacagc aaaatggaaa aacattgact gattatgaag
    26461 taacagatat cgggacccca atcgtgaagc agcaaaaagg aatcaacgag ctcacgcttc
    26521 attatcaaac gccaatctat ttctatttca ttctttctct tactttgatt ggctggttta
    26581 ccttgctatg tttatttatt tatcatagat acaagctttt acgtgcttag gcaaaaggga
    26641 taaatacatc cgatcaaaaa actctccaaa cggtataatc gtttggagag ttttatttta
    26701 atcagttttt ttatgttata tgttatattt tttattcgtt cacatctaat tcgatttcca
    26761 ccccgtagtg atcactgatg attggactat cgatcccatc aaaaatccgt tgatattttt
    26821 taacctgcat tccttcaggg acgaaaataa aatctatacg caatttttct gtattttgtt
    26881 cccaaccatc tattttcttt tccacagtag ctgcttttcc tttttcattc gcgacaacat
    26941 acgaatcttg aagaagaagg caactttctc ttacagtata ataaccttga gagtctactg
    27001 gattattgaa atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag tagttttcca
    27061 agtttttcca ctcaaaataa aagccacttt ttttatctgt ccaccaagaa aagtggcagc
    27121 tcgagacaaa aatgtcctga tcatctatct ttgttttccc aatcaagatc ttgcgtgtct
    27181 gataatcttc tttcttttga aattgggaga cggtaaggac ctgactttcc aatgtttttt
    27241 tcgtaagcag tgcatttcct tcttcatatt tatcataacc gatgtggctc atttcccaac
    27301 tccaaaaata ctcttttccg tgttccttta aaagctgaac taaacggtag gcaaaattat
    27361 ctttacggat aggtacttga ttctttaccg gacagaactt cattagcttc gcaggttcta
    27421 cttcttcaga ttccagtaat tggttgattt cttgtaaagc aatcacttca taatcttcta
    27481 acaggatctt atctgctaat tcttgcaatt ttttctctgg ttctggctca agccagctat
    27541 gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt cttcatttcg aaacaaaagg
    27601 gttgtgacaa aatttttgtc acaacctctc cagcaaatag tgttctcctg ctaactcctc
    27661 agtaataagc tcaatggacc aggagctatt ttttgtttgg ctgtttttat cacttggtgc
    27721 agggcagctt tttcacgacc tctccactaa acagtattca aactgctgct tctcggtaat
    27781 aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tatgacttga
    27841 tgcaggacag ctttttcaca acctctcctt acattttaat ggagccgatc actttaccag
    27901 ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt gtaaatacga
    27961 tcatgatcgt tggatctttt tcttcgtttt ggatcacatc aagatccatc tcagctaatt
    28021 tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct ggctgattca
    28081 tttggactgt atcgattccc atatggatca aaacttcgat accatctttc gtttccagcc
    28141 caattgcatg tttgctaggg aaaatacttg tcacttttcc agcgatcggc gcaacaactt
    28201 ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag aataccggat
    28261 cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt tcttgttcta
    28321 tacctaagta ttgcttacta cctgcttgtc catttcctgt agaaaccatt tctggctctg
    28381 gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca tctgcttttg
    28441 gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc cctgctcgtt
    28501 tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt aaccgagtca
    28561 tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg atctggacca
    28621 cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca ctgtcattgt
    28681 cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag aaatttgcta
    28741 agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata agatctccac
    28801 ctagacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc ccaaatgaat
    28861 ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc actgaataaa
    28921 tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct gttacacctg
    28981 ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct ttcttatcag
    29041 gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa gaaccaatca
    29101 tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca tattgcgaca
    29161 tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat aacggatctt
    29221 gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat ccgccaagct
    29281 gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat agtaaacgtt
    29341 ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat tctgcaatcc
    29401 ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca agaatcaatg
    29461 ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca ttgaaaaatg
    29521 acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt gccccgacaa
    29581 atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc aatacacttg
    29641 taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat gcatcttcat
    29701 tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg attaaaataa
    29761 atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt cctcctattg
    29821 ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt tcaattactc
    29881 cgcccgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat ttccctatac
    29941 tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc tttccaaatt
    30001 tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt acgcaatcgg
    30061 tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat tttttatttt
    30121 tctgtctata ccattctata aaacctataa tacgtgtgtt cccttctata attttctcct
    30181 aaaaatttat ttttttaagc ttttttctat gaaatatttt cttttgtcta ttgacttgtg
    30241 caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga aaaatgaaac
    30301 agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa ttagataaaa
    30361 aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg gggatgcgcg
    30421 ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg gcgggtgtct
    30481 ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa tactttggaa
    30541 aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat cagctgatcg
    30601 acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa aatggtgtat
    30661 tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc ttcgaacgtt
    30721 ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa atgcttgaag
    30781 gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac gaagacagca
    30841 actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa atcggctatt
    30901 tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt actgcagcaa
    30961 tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat actttagcat
    31021 tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg gaaaaagaaa
    31081 ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa gctgtctcag
    31141 aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca gcggcttggg
    31201 cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca caacaaggta
    31261 ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa cgattgaata
    31321 tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg gatacagaag
    31381 cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag aatctgctga
    31441 aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag
    31501 gcgcgcttta tcctatggtc accttcactg gtgtcgaatg ccataacgag tgggaaatca
    31561 cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat tatacgaatt
    31621 acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca gaaatcgcac
    31681 gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg atccacggtg
    31741 tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca aatacgatcg
    31801 cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa gaagctgcta
    31861 tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac atgtacttcc
    31921 cagttgacga agaattagga atctttgtcc agcatgacac attcttagat aaagatctca
    31981 tgcctgtatc cgatattccc acaagcgatc ttcctctgaa tcagcattgg tcttgggaca
    32041 aaatccttag atcttgcttc atcaagcagg cagatgtact acaggggatt tatttcttca
    32101 atgatacttt tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa ccaatgactg
    32161 tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa ttaggaatgg
    32221 aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat aattacaata
    32281 acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg tcgattgtcc
    32341 aaggatttgc tcaaatgaaa actgcccatg atcagttgag attcgcacca tttctgccaa
    32401 aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg ttcatctcag
    32461 tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa atttacgtct
    32521 atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag gaggaagcaa
    32581 atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc tgaattccat
    32641 tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgacca cacattcaac
    32701 gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc ttatggcgga
    32761 aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa aaacgattat
    32821 tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggggt cgtgccttta
    32881 ctggattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag taaaaacggg
    32941 ccattcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc cgacccggca
    33001 gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga
    33061 ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca agccatcctc
    33121 tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga ggagatcccg
    33181 cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg gcatgaccat
    33241 ggataatcca gagatctccg tcgtaccagt tccagagact gaaataaaga aaatcacatt
    33301 gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc accaaatctt
    33361 cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc gtttcgaaga
    33421 tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg gaaggatccg
    33481 ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc atatccacgg
    33541 tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa ctccatcatc
    33601 aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac caatcactgc
    33661 tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg ctagtagcct
    33721 tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag gaaatggaac
    33781 aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg aattagatca
    33841 agaaaaactg cctactggaa attggacaaa aaaagaaaaa ctgccaatcg actttagaaa
    33901 atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg atgtatttct
    33961 tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc attctggtcg
    34021 ccaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga ctggattcga
    34081 agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag caatcgaacc
    34141 gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc tccatcctgg
    34201 tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag agaaaggtgt
    34261 ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac
    34321 cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga agaaagtccg
    34381 aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc taggaaaacg
    34441 catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc ggatcggtaa
    34501 cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt atgatatgac
    34561 tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc agcgtatgca
    34621 tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag atactgtcat
    34681 tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt atcatcatga
    34741 gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag caacagaatt
    34801 tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc atgaaagttt
    34861 gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt
    34921 atccagtgta gtactagagc atccggagga ttatctgtcc tttgagaaaa cgttgaaaga
    34981 aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca cgatgcagtt
    35041 ggcgcaaatg tatggctatg tcattccaga gacactctcc gtcattagct tcaacaactc
    35101 gatcttctca acattaacac acccttacct tacaagtatc gatatcgata tctccgaatt
    35161 aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga gcagtggcgt
    35221 acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc
    35281 atactaaata gctgactcac taaatacgct gaccatttat cctatggtca gctttttatg
    35341 taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt gggacaaaag
    35401 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    35461 cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt
    35521 atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca
    35581 cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa
    35641 aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg
    35701 ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc
    35761 aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga
    35821 atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta
    35881 aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca
    35941 acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca
    36001 gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt
    36061 gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc
    36121 tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc
    36181 ggaatttttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag
    36241 atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa
    36301 tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc
    36361 gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag
    36421 ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg
    36481 cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca
    36541 atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga
    36601 aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta
    36661 gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg
    36721 attggaatga tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg
    36781 aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt
    36841 ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca
    36901 gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa
    36961 aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa
    37021 cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc
    37081 caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata
    37141 gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg
    37201 atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca
    37261 attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca
    37321 atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca
    37381 gcacttacgt catccattac accataaagc aaagcaaata atgaaataac gattgattgt
    37441 gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt
    37501 ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct
    37561 gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct
    37621 ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt
    37681 tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat
    37741 ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc
    37801 atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc
    37861 aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag
    37921 ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact
    37981 actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat
    38041 ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag
    38101 tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa
    38161 gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc
    38221 catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc
    38281 gtggaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt
    38341 gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca
    38401 cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta
    38461 ctcaccgttg acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt
    38521 cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga
    38581 agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc
    38641 tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt
    38701 accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt
    38761 cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt
    38821 atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat
    38881 ctgagaatcc tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa
    38941 agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat
    39001 aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt
    39061 tgaatacata gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt
    39121 tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga
    39181 ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg
    39241 cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta
    39301 atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc
    39361 tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag cccattttat
    39421 gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt
    39481 ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat
    39541 cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat
    39601 aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg
    39661 caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta
    39721 cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga
    39781 tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga
    39841 tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta
    39901 atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg
    39961 ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc
    40021 tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt
    40081 tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc
    40141 agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc
    40201 ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt
    40261 acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat
    40321 tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg
    40381 ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca
    40441 tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca
    40501 aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta
    40561 gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt
    40621 cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa
    40681 tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa
    40741 acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt
    40801 ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta
    40861 aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg
    40921 aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt
    40981 aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt
    41041 agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat
    41101 ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat
    41161 atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg
    41221 cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt
    41281 ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa
    41341 gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga
    41401 agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc
    41461 agccttcagc atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt
    41521 tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa
    41581 ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt
    41641 aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat
    41701 cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga
    41761 caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat
    41821 ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc
    41881 tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg
    41941 atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg
    42001 catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag
    42061 ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc
    42121 aataatattc attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc
    42181 atatgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat
    42241 aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac
    42301 ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt
    42361 cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac
    42421 aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa
    42481 ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg
    42541 aatccgctct aattccgaac attttatttt caattattgt taaaagaaat aactttttct
    42601 gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt
    42661 acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa
    42721 aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt
    42781 ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata
    42841 acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat
    42901 ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac
    42961 ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc
    43021 gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg
    43081 caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa
    43141 gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat
    43201 gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa
    43261 ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata tatggataat
    43321 tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa
    43381 gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc
    43441 aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac
    43501 aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa
    43561 aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt
    43621 gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa
    43681 gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt
    43741 tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt
    43801 gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct
    43861 tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt
    43921 tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat
    43981 gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag
    44041 ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg
    44101 attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc
    44161 tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa
    44221 tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg
    44281 aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta
    44341 tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg
    44401 ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc
    44461 gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg
    44521 ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc
    44581 attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc
    44641 tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat
    44701 tttaaacttt tataatatat atagttcaaa tttattctga ggtttattat aactatctat
    44761 tgagaaagta tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa
    44821 agaatattat gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat
    44881 taaactattt aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca
    44941 gaaatcgtca aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat
    45001 gtctctttca ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga
    45061 aaatccacga cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg
    45121 atttttgggc aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt
    45181 ccaggcgata tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg
    45241 aagcttcatg gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt
    45301 gatcctaaga aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt
    45361 gctgctttgg ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat
    45421 cctttgatgg aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc
    45481 atcctacttt cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc
    45541 atacgcgaag gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt
    45601 tcgaccatca ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc
    45661 catgactttg tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat
    45721 gtgattttag ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact
    45781 cttgaggatt tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa
    45841 ttttcacgct ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg
    45901 atatggctag tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg
    45961 acttccggac aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca
    46021 atggtcggac caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca
    46081 aatgaaatgt tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg
    46141 agccaaacga gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc
    46201 ggacgtcaag caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta
    46261 gcaatatcga tcagctgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt
    46321 gcttggcttt ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg
    46381 tttgctgcac aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta
    46441 ggtggacttt accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg
    46501 aatcctttag gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg
    46561 ctttatttgt ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat
    46621 cgagatttga acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct
    46681 cttttatcct tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt
    46741 atcacttttg gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt
    46801 ttagaaagta acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa
    46861 aactttacaa gcacgatcat ggtagtcttg atttgtctag ctgctatttt accaattgct
    46921 agcatcaatc gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca
    46981 aaaatttcta ggaaccaact ttattggacc aatatgttac tagctattct atttgctata
    47041 ttggcaatcc tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat
    47101 gcgaaagaca tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt
    47161 ttagtgttta tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt
    47221 gcttatatct acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta
    47281 ccagaatggt tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct
    47341 tttgatcttt ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt
    47401 tctattggtt ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc
    47461 atgctagcct ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca
    47521 agtcttctaa ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac
    47581 cattctcctc taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg
    47641 cagcaagttc tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta
    47701 agtcaagcag atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct
    47761 gctgctgtac ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg
    47821 gataatgcag taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt
    47881 ctagtgcttc gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat
    47941 atccgcccgg ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca
    48001 ctttcatact gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct
    48061 gttctccttt ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct
    48121 gtactaagtg attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct
    48181 ctcctccttc taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat
    48241 aattttcttt tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat
    48301 ttctttttgt cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc
    48361 aattttttaa atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga
    48421 gtaaggtttg tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta
    48481 gcttttgata tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt
    48541 cgatttcttg cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt
    48601 ttttcaactc ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt
    48661 caagttcatg ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc
    48721 actctttcag tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc
    48781 ttttataatg ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta
    48841 gacaggcatt ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta
    48901 aattccagcc attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca
    48961 aaagtgcagc tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt
    49021 tcttgatttg ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga
    49081 ttggtcgatc aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg
    49141 ttatcttttg tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct
    49201 gttctgcatc aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt
    49261 ttattgaact taaggttgat tgatcacatc taaaagcgtt gaaaataaaa tggcactaga
    49321 agcagctcct gggtcgatat gtccgattgc acgctctcct agataggaag ctcgtccttt
    49381 tttagctacc aagactttgg tgtgttcttt tgcctggtca atgatttctt ttgttaaatt
    49441 ccctgccttc aatgcctctg tgactggcag ccaaacatct aacatcgttt tatcgtcagc
    49501 ttctgccttt cctcggcttt gaatacccgc tgttccctgc tcgatcactt cgcctaattt
    49561 ttcttgagaa tcaatagaat cgacctcttt tgtcgctttc atcatgttca tgaatgcaga
    49621 tccgtataac ggaccagaag ccccaccgac tttcgatatc atcgccatag agaatacttt
    49681 gaatgtatcg ctgattgttt caggatgatc ggcttgaaaa gcttcttcat aagctgaaac
    49741 ccctcttccc atgttatttc catggtctcc atcgccaatc ggggtatcta gatcactgag
    49801 atattgtttg ttctgattga ttgcctctgc aaattgcgtg agccattctt ggatttcttg
    49861 aacggttagt ttcatattat caccctttta tcttgagatt ttttaccatg aaatcgtctg
    49921 tacattgctt tctaaagaat ctttccattg tgtctccgtc aagtcgatca gtgttagaga
    49981 aagaccttgc atatctagtg aggtcataaa attaccgact tttcggaaaa ctacattgac
    50041 gcctctttct tccaacagag ctaaaacatc attcatgaac acgaactgtt ccatcaaagg
    50101 cgtcccgccc attccgttga ctagtacacc tgcttctttt ggtaaattcg agtaatcatc
    50161 taaaatctta gtaacaagtt cctttgccag tactttagaa ggctgcatct tttctcgacg
    50221 atagcctggc tcaccgtgga taccaacacc atactcgatc tcatcttctc ctagttcgaa
    50281 tcctggtttc cctacttctg gtacagttgc tgctcgaagc gctagaccga tcgtcttagt
    50341 tgcctgtacg accttttctc ccagttcttt caactcagct agggaagcac cgtttcttgc
    50401 tgcatcgccc acgattttgt gtacaaggac agtacctgca actccgcgtt ttccagcagt
    50461 atacgtacta ttttcaacgg cgatatcatc atctacgatc acactttcta cttcaatatc
    50521 gtccatagca gctaattcct ttgccatatc gaagttcaat gcatcccctg tataattctt
    50581 aacgatcagc aaaactcctt ttccactatc ggcttcctta atagccgtct gaatctgatc
    50641 tggagttgga gaagtaaaca catcaccaag caccgctgca cttaacatcc cttcccctac
    50701 aaacccggca tgggatggtt catgtccgct tcctccacca gaaaccaaac caacttgttc
    50761 cgctttctga ttcttggcta caacacggga agattctaca cgatgaacaa gttctggata
    50821 gctttttact aatccttcca gcatttcttc gacaatattg cttggatcat tcatgatttt
    50881 tttcaattta ttcccatcct ttctttccgg cattttgtgc ttctgttaag atttgctgag
    50941 tatcatctgt tataccggca gtgatggctg ctgcaaaagc tccttccact aaaggcgcat
    51001 ccaccagatg ataacgttgc tgctgctctt cttccaacat gtcttttgcc agttccgagt
    51061 tcaataccgc gctaccgata tccgcaaaaa caaaataact agctgcatcg cttgattcat
    51121 tgactgcttc tacgattttc atcgggtctg atccgatctc gccatttatt ccgccgagag
    51181 aaaaaatttg cacctcttcg gatttttgca tctgctcaat catttcttta ataccatctg
    51241 ttactttttg actatgagaa ataagcaaaa tacttttttt cacaagaatc ttcctttctt
    51301 ttacatagtt gctaacgatt ggtttcgctt tcattaatga acaattcaag tatataataa
    51361 atttctaatt ttgggaaaat atgtgtagtg cttcttgaag tttgttatac tttaattaaa
    51421 agagaggagt gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt
    51481 agcttataaa gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt
    51541 ggaggcgggg ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat
    51601 gggtgcaaga gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga
    51661 tgcgatcatt ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat
    51721 ggtcacatgt agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa
    51781 ctaaagagag atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga
    51841 ggtactctac cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg
    51901 ctgcgactaa cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg
    51961 tttttggcaa agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg
    52021 tcgtgcttcc agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt
    52081 ggaaactagc tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt
    52141 cgctgatctt tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta
    52201 gctttccaga agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt
    52261 ggatcttccc gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct
    52321 ttttctcaag ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat
    52381 cgattgctgc ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt
    52441 cttttctttt attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat
    52501 ttattgatgt atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg
    52561 aagtcatttc tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag
    52621 gttctgtttg agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac
    52681 tggaaacagc aattggccag attaacggct tcattctttt tttcatcact tttcccatcc
    52741 ttactacttt ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac
    52801 aacaggctgt ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt
    52861 ccatgtcccg cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat
    52921 cgttatcgcg ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt
    52981 caattttctt cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac
    53041 catttcaaag cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt
    53101 tccttgatat acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa
    53161 tattgaccag acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt
    53221 aaaaggatgg agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc
    53281 ccaacgtttt ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat
    53341 aatctcagga gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat
    53401 gtcttctgtc gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata
    53461 catcgtattt gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt
    53521 aatgatgccc acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg
    53581 attttggaca tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa
    53641 ataattcaca tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa
    53701 aatcaatttc ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt
    53761 tttttcgatc cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc
    53821 tggtgtttgg tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa
    53881 tttcgtcatc aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac
    53941 catgatcgtg atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg
    54001 tttgtataaa aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg
    54061 ataagaaacg agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt
    54121 atgggtgatt gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat
    54181 gtcgtatcta gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct
    54241 tttgttccaa aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt
    54301 tcatcttcat attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta
    54361 gtcaatacaa atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag
    54421 aaatacagca ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct
    54481 ggtctcgtta atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa
    54541 agagccacta acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct
    54601 ttcaccaaaa ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt
    54661 tttttgttac gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct
    54721 aaatttttcc taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt
    54781 ctttttatca gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg
    54841 acaatgcttt tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca
    54901 tctccatcgt tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga
    54961 ctcaaaggaa tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt
    55021 agcgtttggg tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa
    55081 accaaaatct cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg
    55141 ggttcttttc cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag
    55201 gttttgaaaa gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga
    55261 tggatcggca gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa
    55321 ttaaagattg cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg
    55381 atcaatagac ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga
    55441 aatgctggta catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct
    55501 tcatcaacag tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt
    55561 ctttcaaaca actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt
    55621 cgatattttt tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca
    55681 gaaatcaatt taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc
    55741 tgcacagcag gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc
    55801 tttttcgttg cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt
    55861 ttttcgatcg tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa
    55921 ttcggatgat ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta
    55981 cgcaaacgtt ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct
    56041 tcaccttcta tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt
    56101 ggatttcttt cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta
    56161 ggtgatagaa tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac
    56221 gcaatacgct gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata
    56281 ttttgacttt tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata
    56341 gccttcattt cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata
    56401 gctacagacg tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt
    56461 tctttaataa ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg
    56521 ttattgtaaa aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca
    56581 tcatctgtga aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag
    56641 tctacaacga cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga
    56701 ttttctgcgt ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc
    56761 atcgaaaaag tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc
    56821 atttctttca acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg
    56881 ttttttttat caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt
    56941 tgtgtgttga ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc
    57001 tcttttaaga gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc
    57061 ttcaggtcgg acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc
    57121 agactgcttc aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat
    57181 atattcttgt tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg
    57241 tttggcttcc ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat
    57301 ttttcctttt gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc
    57361 tgcaatgttc attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg
    57421 cggtgcatac gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc
    57481 tgttgctaaa atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata
    57541 aatctcgcca gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata
    57601 atagcttgca tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt
    57661 ggctgttctt tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc
    57721 tgttccaata ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct
    57781 accttgacga ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg
    57841 ttttacaacg atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc
    57901 gtctactagg atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt
    57961 atcaggacga acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc
    58021 cagtcgcaat cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc
    58081 tactccttga tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg
    58141 aagaatatgt ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc
    58201 tgccatttcc aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc
    58261 taatgcatgc atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc
    58321 ttctgttatc cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc
    58381 atcgtactgt tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg
    58441 aaagatggct gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga
    58501 ttctggcgtt tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg
    58561 caagccgcaa tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc
    58621 ttccattaat cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat
    58681 aacgattttc atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac
    58741 gtacattcac tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca
    58801 tccctgattg acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac
    58861 tttgtaatgg aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc
    58921 taacatcaag taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg
    58981 attttattga ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac
    59041 ctcatggggt atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt
    59101 ttttcactac tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa
    59161 atagaggaga atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta
    59221 cagagggaca aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg
    59281 tcatcactca attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg
    59341 cagataatct gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta
    59401 aactagaaca agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc
    59461 gttttccata ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg
    59521 ataaaagctt ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt
    59581 tatctagact ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc
    59641 gccaatgcta tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt
    59701 gtaccagcag gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag
    59761 ccttagtcag tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt
    59821 cgaagaagct acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca
    59881 agt
//
LOCUS       DACERL010000009        58555 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.009, whole genome shotgun sequence.
ACCESSION   DACERL010000009 DACERL010000000
VERSION     DACERL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58555
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.009"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            121..1557
                     /locus_tag="IXF54_000545"
     CDS             121..1557
                     /locus_tag="IXF54_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1403830.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1746..4193
                     /locus_tag="IXF54_000546"
     CDS             1746..4193
                     /locus_tag="IXF54_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1403831.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4423..5490
                     /gene="rlmN"
                     /locus_tag="IXF54_000547"
     CDS             4423..5490
                     /gene="rlmN"
                     /locus_tag="IXF54_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1403832.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5683..6786)
                     /locus_tag="IXF54_000548"
     CDS             complement(5683..6786)
                     /locus_tag="IXF54_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1403833.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7339..7887
                     /gene="aac(6')"
                     /locus_tag="IXF54_000549"
     CDS             7339..7887
                     /gene="aac(6')"
                     /locus_tag="IXF54_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1403834.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8433..10094
                     /locus_tag="IXF54_000550"
     CDS             8433..10094
                     /locus_tag="IXF54_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1403835.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(10164..10511)
                     /locus_tag="IXF54_000551"
     CDS             complement(10164..10511)
                     /locus_tag="IXF54_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1403836.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            10595..11536
                     /locus_tag="IXF54_000552"
     CDS             10595..11536
                     /locus_tag="IXF54_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403837.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            11537..12580
                     /locus_tag="IXF54_000553"
     CDS             11537..12580
                     /locus_tag="IXF54_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403838.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            12586..13656
                     /locus_tag="IXF54_000554"
     CDS             12586..13656
                     /locus_tag="IXF54_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403839.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            13646..14599
                     /locus_tag="IXF54_000555"
     CDS             13646..14599
                     /locus_tag="IXF54_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403840.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            14611..15621
                     /locus_tag="IXF54_000556"
     CDS             14611..15621
                     /locus_tag="IXF54_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1403841.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(15906..16727)
                     /locus_tag="IXF54_000557"
     CDS             complement(15906..16727)
                     /locus_tag="IXF54_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1403842.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(16824..17102)
                     /locus_tag="IXF54_000558"
     CDS             complement(16824..17102)
                     /locus_tag="IXF54_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403843.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            17370..19379
                     /gene="metG"
                     /locus_tag="IXF54_000559"
     CDS             17370..19379
                     /gene="metG"
                     /locus_tag="IXF54_000559"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1403844.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(19858..20445)
                     /locus_tag="IXF54_000560"
     CDS             complement(19858..20445)
                     /locus_tag="IXF54_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403845.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            20620..21942
                     /locus_tag="IXF54_000561"
     CDS             20620..21942
                     /locus_tag="IXF54_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1403846.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            22097..22873
                     /locus_tag="IXF54_000562"
     CDS             22097..22873
                     /locus_tag="IXF54_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1403847.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            22866..23435
                     /gene="rnmV"
                     /locus_tag="IXF54_000563"
     CDS             22866..23435
                     /gene="rnmV"
                     /locus_tag="IXF54_000563"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1403848.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            23428..24312
                     /gene="rsmA"
                     /locus_tag="IXF54_000564"
     CDS             23428..24312
                     /gene="rsmA"
                     /locus_tag="IXF54_000564"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1403849.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            24763..25392
                     /locus_tag="IXF54_000565"
     CDS             24763..25392
                     /locus_tag="IXF54_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403850.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(25516..26625)
                     /gene="ugpC"
                     /locus_tag="IXF54_000566"
     CDS             complement(25516..26625)
                     /gene="ugpC"
                     /locus_tag="IXF54_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1403851.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            26834..27256
                     /gene="mgsA"
                     /locus_tag="IXF54_000567"
     CDS             26834..27256
                     /gene="mgsA"
                     /locus_tag="IXF54_000567"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1403852.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            27579..28115
                     /locus_tag="IXF54_000568"
     CDS             27579..28115
                     /locus_tag="IXF54_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1403853.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(28155..29918)
                     /locus_tag="IXF54_000569"
     CDS             complement(28155..29918)
                     /locus_tag="IXF54_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403854.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(29921..31636)
                     /locus_tag="IXF54_000570"
     CDS             complement(29921..31636)
                     /locus_tag="IXF54_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403855.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(32415..32639)
                     /locus_tag="IXF54_000571"
     CDS             complement(32415..32639)
                     /locus_tag="IXF54_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403856.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            32839..35607
                     /locus_tag="IXF54_000572"
     CDS             32839..35607
                     /locus_tag="IXF54_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1403857.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            35688..36107
                     /locus_tag="IXF54_000573"
     CDS             35688..36107
                     /locus_tag="IXF54_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1403858.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            36119..36589
                     /locus_tag="IXF54_000574"
     CDS             36119..36589
                     /locus_tag="IXF54_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1403859.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            36606..37385
                     /locus_tag="IXF54_000575"
     CDS             36606..37385
                     /locus_tag="IXF54_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1403860.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            37372..38193
                     /locus_tag="IXF54_000576"
     CDS             37372..38193
                     /locus_tag="IXF54_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1403861.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            38210..39259
                     /locus_tag="IXF54_000577"
     CDS             38210..39259
                     /locus_tag="IXF54_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1403862.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            39272..40276
                     /locus_tag="IXF54_000578"
     CDS             39272..40276
                     /locus_tag="IXF54_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1403863.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            40909..41928
                     /locus_tag="IXF54_000579"
     CDS             40909..41928
                     /locus_tag="IXF54_000579"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1403864.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            41971..42852
                     /gene="galU"
                     /locus_tag="IXF54_000580"
     CDS             41971..42852
                     /gene="galU"
                     /locus_tag="IXF54_000580"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1403865.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            43152..43529
                     /locus_tag="IXF54_000581"
     CDS             43152..43529
                     /locus_tag="IXF54_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403866.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            44135..44749
                     /locus_tag="IXF54_000582"
     CDS             44135..44749
                     /locus_tag="IXF54_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1403867.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            45522..47261
                     /gene="yfcC"
                     /locus_tag="IXF54_000583"
     CDS             45522..47261
                     /gene="yfcC"
                     /locus_tag="IXF54_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1403868.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            47418..48602
                     /gene="iadA"
                     /locus_tag="IXF54_000584"
     CDS             47418..48602
                     /gene="iadA"
                     /locus_tag="IXF54_000584"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1403869.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            48713..48916
                     /locus_tag="IXF54_000585"
     CDS             48713..48916
                     /locus_tag="IXF54_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1403870.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(49017..49679)
                     /locus_tag="IXF54_000586"
     CDS             complement(49017..49679)
                     /locus_tag="IXF54_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403871.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(49692..51194)
                     /locus_tag="IXF54_000587"
     CDS             complement(49692..51194)
                     /locus_tag="IXF54_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403872.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            51742..53421
                     /locus_tag="IXF54_000588"
     CDS             51742..53421
                     /locus_tag="IXF54_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1403873.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            53548..54027
                     /locus_tag="IXF54_000589"
     CDS             53548..54027
                     /locus_tag="IXF54_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1403874.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            54246..54926
                     /locus_tag="IXF54_000590"
     CDS             54246..54926
                     /locus_tag="IXF54_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1403875.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            54934..56265
                     /locus_tag="IXF54_000591"
     CDS             54934..56265
                     /locus_tag="IXF54_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1403876.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            56280..57242
                     /locus_tag="IXF54_000592"
     CDS             56280..57242
                     /locus_tag="IXF54_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1403877.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            57353..57691
                     /locus_tag="IXF54_000593"
     CDS             57353..57691
                     /locus_tag="IXF54_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1403878.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            57770..58477
                     /locus_tag="IXF54_000594"
     CDS             57770..58477
                     /locus_tag="IXF54_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1403879.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT        19141 a        10211 c        12251 g        16952 t
ORIGIN      
        1 ctactcgaaa ctaagcgact ttttcacaac ctcctttaca taattttatt ttgttgtgga
       61 aggggtttcg tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga
      121 atggaacaca aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca
      181 gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga
      241 tttgtacgga tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac
      301 tatcatcgtt ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc
      361 ttctctgtag cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg
      421 ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg
      481 actttatatc attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca
      541 cgtcagatca ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg
      601 aaagtccatt actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg
      661 ttagcagcac atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt
      721 gctaatttag caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa
      781 atcggaccaa gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt
      841 ctggctgctg aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg
      901 ggaaaatatc cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt
      961 gaagctggcg atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac
     1021 tatcaaacgg cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat
     1081 acgacaggaa aaaaaggttc atcagaagaa actggagttc ctggaatgta taaaaaagtt
     1141 gagaatccat ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg
     1201 atcggactgc gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc
     1261 ttaggagagt atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat
     1321 tacttacaaa gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta
     1381 ggctattgca cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga
     1441 tatggatttg tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag
     1501 aaagacagtt attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc
     1561 acatacttaa aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat
     1621 ttagaaatta agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc
     1681 aaacaaaact ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat
     1741 aataaatggc acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa
     1801 agcaaaaaca gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat
     1861 ttgctgtctt cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc
     1921 ggatcattgc aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt
     1981 ggattgtcat taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca
     2041 tactctttta tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata
     2101 ctggggaacc aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa
     2161 gtcaagtaga caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt
     2221 acttcttgat tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag
     2281 cattcctgtt cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag
     2341 aacgagtaca ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa
     2401 aggaagagag aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag
     2461 aagctgcaga aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg
     2521 aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg
     2581 ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag
     2641 atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg
     2701 gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga
     2761 cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg
     2821 aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg
     2881 tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa
     2941 aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg
     3001 tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa
     3061 agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat
     3121 tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga
     3181 agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg
     3241 ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg
     3301 atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg
     3361 tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac
     3421 gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc
     3481 aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg
     3541 tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc
     3601 gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa
     3661 gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg
     3721 tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag
     3781 ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag
     3841 cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag
     3901 ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac
     3961 agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa
     4021 gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag
     4081 atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat
     4141 tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt
     4201 gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag
     4261 aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt
     4321 gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg
     4381 tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt
     4441 tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc
     4501 cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg
     4561 acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta
     4621 aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct
     4681 gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg
     4741 acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag
     4801 aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt
     4861 gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca
     4921 tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg
     4981 gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa
     5041 ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag
     5101 gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct
     5161 gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat
     5221 caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa
     5281 aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct
     5341 cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac
     5401 tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc
     5461 aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat
     5521 aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat
     5581 agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct
     5641 caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata
     5701 tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa
     5761 tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc
     5821 taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag
     5881 ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat
     5941 caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc
     6001 tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac
     6061 taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata
     6121 tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc
     6181 aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg
     6241 agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta
     6301 tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga
     6361 aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac
     6421 gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc
     6481 aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt
     6541 ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta
     6601 ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc
     6661 ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat
     6721 tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg
     6781 actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga
     6841 tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag
     6901 gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca
     6961 ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt
     7021 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
     7081 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
     7141 gagttttttc ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct
     7201 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
     7261 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
     7321 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
     7381 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
     7441 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
     7501 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
     7561 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
     7621 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
     7681 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
     7741 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
     7801 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
     7861 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
     7921 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
     7981 ttccgaagaa gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt
     8041 tcgagtagta agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt
     8101 aataccgaga aactggcttt tgaacaccgt ttattcagat aagggactgt gacaagactt
     8161 ttgtcacagt ttctttttgt ttattctttt ttatctttgt atggcaataa tagacaatat
     8221 aacgaaaatt atttaatggc ttttggaaga ccatggaaaa ccttgtacaa agctattttt
     8281 tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa
     8341 agtgttgatt ttttaataat tattcattaa actcttaaac aacttcagaa aatattaatt
     8401 attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg
     8461 ttgcggtatg cggccttgta cttgctggat gttatggcgg cagttcagcc acaagcagta
     8521 gttcagcaaa aagcggttca gcagatggag gaggcgtatt taacttagtc gtgcctcaag
     8581 aaatgcctac agctgactta tcagttgcaa cagatacaat cagttttaca gcattgaata
     8641 acgtttatga aggaatttac cgtcttgatg aagacagcaa accacagccg gcaggagcta
     8701 gcgagttggc agaagtcagt gaagacggat taacttacaa aatcaaatta cgagaagacg
     8761 caaaatggtc aaatggtgat cctgttacag cagcggatta cgtttatggc tggcaacgaa
     8821 cagtagaccc agctacagct tctgaatatg cttacctgta tgcgccagtt gaaaatgcag
     8881 aagacattac agctggaaag aaagataaat cagaactagg gatcaaagca gttggtgact
     8941 atgaattaga aatcaaattg acgaaacaaa caccatattt ccaatatctg cttgctttcc
     9001 catcattttt cccacaaaaa caatccgtag tggaagaaaa tggggatgcc tatgcatctg
     9061 caagtgataa agctgtttat aatggaccat tcacattaga agattttgat ggaccaggaa
     9121 cagatacaga atggacttat aagaaaaatg atcattactg ggacaaagat acagtaaaac
     9181 tttctgaaat caaagtcagt gtagtaaaag aatcttctac tgcattgaac ttattcaaag
     9241 atggccaagc agatgacgtg atcttgtctg gtgagttagc acagcaaaac gcaaacgatc
     9301 cagcttatac ttctgttaaa gaagcgcgga caagctatat cgaattcaat caacgtgaaa
     9361 agaattcacc attcaacaat gtgaacctaa gaaaagctat ttcttattct atcgatcgtg
     9421 aagctttagt aaaacaagta ctaggtgacg gatcagttgt ctctacagga ttgatcccat
     9481 cagatatggt aaaaagtcca acaacgaacg aagactttgc aaaagaagct ggaaaattag
     9541 tttcttatga caaagataaa gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg
     9601 ttgattcttt agaatttgat ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg
     9661 aatatgttca aaactcaatc caagaaaact tggatggcgt aacagtcaaa ccaacacctg
     9721 ttccattctc tgtacgtctt gaccgctcaa cttcgggtga ttttgacgca gtactaggcg
     9781 gatgggctgc tgactatgca gacccaagca gctttactga tttgtttgtc acaggtaatt
     9841 catacaaccg tggtcgctgg agcaatgcag aatatgacga agcagttaaa gcagcttcat
     9901 caacagacgc tggcgatgaa caagcccgtt gggatgattt ccaaaaagca atcaagatca
     9961 tctctgatga tatgggtgtc gttccggtgt accaaaaagc agaaggtcac ctagtgaatg
    10021 aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc atgggattac aaatggacat
    10081 acgttgaaga ataatcgtta ataccgtgag catgagaaaa aacgaggctg cggcaaatgt
    10141 cgcaacctcg ttctcatatt cacttatcct aacaaagaaa aaagcatgaa aatagcagaa
    10201 agagcaatta gtattccggc aatgatcagc caaaagctgt acataccgta gctggcagac
    10261 gagagcgagg aaaattctgg ttttttcttt cggttccttg ttcgtgcaag atggaccgat
    10321 cgctgaaaat gatcgaagaa acccgaagaa aagacccata aaaaaccgcc gataatcaga
    10381 aacggcaatg accataagaa tagatcatta gataattgaa tgagtgtaaa ggaatggttg
    10441 atgagatttt ttattaaaat aaccagaatc agtagaaaag ctactgcgta ggggatagat
    10501 ttttttttca tttgattgct cctttatttt tggttcatta tctaataatg tagcgaaaca
    10561 tctgtcagaa gtcaaaagct agggggaaac attagtgaat agttatatca aatatgtatt
    10621 aaaacgtgtc tttttcatga tcatcacgct ttggttaatt gcaacaatca cctttttctt
    10681 gatgcaattg ctgccgggca cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat
    10741 cgagatgcta aataaacaag tcggtttgga taaaccagtt atcgtacaat atgggattta
    10801 tctttctaat ttgcttcaag gagattttgg aatttccttc caatttaaaa accagccagt
    10861 cgcacatttg ttagctggaa gaatcggacc atccttgcag ttagggttac aggcaattat
    10921 ctttggaaca gtcctaggga cgattttagg aacgatctct gccatgaagc aaaatacatg
    10981 ggcagataca tcttctacac tcgtagcgat attaggtcgt tccattccaa actttgtatt
    11041 tgctgtttta ttacaatata tctttgcaat caaattacaa gttttgccaa tcgcaaaatg
    11101 ggatggcttt gtgtacacaa tcttaccaac aattgcgttg gccatgtcgc cgcttgctga
    11161 ctctgctcgg tttatccgta cggaaatggt ggaagtactg catagtgatt atgtggaatt
    11221 agcaaaagca aaaggattga gccgttggga aatcgccttt aaacatggtc ttcgaaatag
    11281 tttgattcca ttgatgactt tattaggacc gttagctgta gcattgatga ccggatcact
    11341 ggtggtagaa aatatctttg ctattcctgg tatcggggaa caattcgtta agtcaatcac
    11401 aacaaatgat tatcctacga tcatggctgt aacgatcctt tactcattta tgctgatttt
    11461 tgttattttg atcgtggatc tattgtacgg tctagttgat ccaagaattc gtgtttcaga
    11521 agggagccga ggataaatgg aaatgatacc aaagcgttat gaaacagttg cagatatccc
    11581 tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa gaacgtgaac agattactgc
    11641 accttctttg aattttatcc aagattcatg gcgccgttta aagaaaaaca aagctgccgt
    11701 tatttcaatg gttttactat tggtcatcat tttgatctct gtgatcacaa tctttgtttc
    11761 tcctcatgat ccaactgccc aaaacgtggc ttatatcaat ttgcctcctc gtattccggg
    11821 aatcaatatc gatggattga atggaaaagc aatggttggt ggtgaacttg ttgataaata
    11881 tgctcaagca aacgtccctt caaacgtaaa tttcttttta ggaacagacg gtttgggacg
    11941 tgatgtatta agccgcttat ttatgggaat gagaatctcg ttgctgatcg catttattgc
    12001 tgcattacta gatgtaacga ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg
    12061 acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta tcaggaattc caaacctcgt
    12121 tgtcatgatt ttgatgttgg tcgtttttga accaggaatt ttttcaatcg tggctgctat
    12181 ggccatcaca aactggatac caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa
    12241 agatcaagaa tatgttttag ctggattgac attaggtgaa tcaagaatga aaattgcatt
    12301 caaacatatt ttgccgaata tctctagtgt cattattatt caaatgatgt ttagtattcc
    12361 atctgctatt ttcttcgaag cctttttaag ctttatcggt ttaggtctta cgcctccgtc
    12421 agcttcatta ggaacgatgt tgagcgaagg gtacaagaca ttcctgtatc tgccttatct
    12481 attatggatt ccggcagtaa ctttatcggt tatcatgatt ggctttaact tacttgctga
    12541 tggattgcgt gatgcctttg atcctaaaat gaaagagtga agacgatgac gaaaatttta
    12601 gaagtgaaag acttagaaat ctcttttgat acgtatgctg gaaaggtacg agcgattcgc
    12661 ggtgtcaatt ttcatttgaa taaaggagaa acattggcga tcgttgggga atctggaagt
    12721 ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt caagcaacgc caatatagat
    12781 gctggtcaaa tcctctttaa aggtaacgat atcgttggaa aatcagaaag agagatgcaa
    12841 aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc caatgacctc tttagatccg
    12901 acgatgccca ttgggaagca agtcgctgag tcccttagaa agcataataa agtttcaaaa
    12961 aaagagggac aaaaagctgc attagaattg cttaaattag ttggaatacc cgaagctgag
    13021 aaacgtatca acagttatcc gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc
    13081 gcgattgcac tggtatgtta tccggaaatc ctgattgctg atgaacctac aacagcgctt
    13141 gatgtgacta ttcaagcaca gattttggag ctgctgaaag aaattcagac aaaaatcgat
    13201 acatcaatta tctttatcac tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc
    13261 gctgtcatgt atggaggaag aatcgttgaa gttggaacat cggaagaaat tttctacaac
    13321 ccgcaacatc cctatacttg gggactgctt ggttcaatgc cgacattaga tagtgcgaac
    13381 gatcgattgt atgctattcc tggttctcca ccggatttat tgaacccgcc aaaaggagac
    13441 gcattctatc caagaaatga attcgcgatg aaaatcgatg cagaacaaga accaccattt
    13501 tttgaactat caaaaacaca caaggcagca acttggctac ttgctccgca ggcacccaaa
    13561 gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat
    13621 acttacggtc aaggaggaat tgctgatgcc aaataaacta cttgaagtta aaggattgaa
    13681 acagtatttc aatgtaggaa aaaagaatga agtacatgct gtaaatgaca tcagctttca
    13741 catttatgaa ggagaaacat tcggtttagt tggagaatct ggaagcggaa aatccactac
    13801 tggccgtacg atcattcgct tgaatgaacc tactgatggt gagatattat tcgacggtca
    13861 agatataacg aagatcaaag ataaaaaagg tttgacgaaa ttccggcatg atgttcagat
    13921 gatctttcaa gatccatacg cttcgttgaa tcctcgtatg aaagtcagag atatcattgc
    13981 cgaaggaatt gatgttaatg gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga
    14041 tgatctattg agaactgttg gattgaatcc aagccacggt actcgttatc ctcatgaatt
    14101 ttcaggtggg caacgccagc gtattggtat tgcacgagca ttggcagtaa aaccgcgttt
    14161 tatcatttgt gatgaaccaa tttctgcact agatgtgtcg atccaagccc aagtggtcaa
    14221 tttgttgcaa gatctccaaa aagaacatca attgacctat ctatttattg cacatgattt
    14281 gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg cataacggct tgttattaga
    14341 gatgggaaca agtgatgaaa tctataatca cggtgttcat ccttatacag aaagtttgtt
    14401 atctgctatt cctctaccag atccagacca tgagagaaaa cgcagaagaa tcaaatatca
    14461 acctgaacca gatgacggac aagtacgtca acttcgtgaa atcgcaccag aacattttgt
    14521 ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc
    14581 tcttctggtt gctaactagg agggacatag atgctaaaag cttttaaatt ccgtatgtat
    14641 ccgacagaag aacaaaaaca gcaattgatt cgtacttttg gatgtgctcg atttacctat
    14701 aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa caggagtagc caatttttcg
    14761 ctcacacccg ctacattaaa aaaagaatac ccattcttaa aagaagtaga cagtcttgct
    14821 ttagcaaatg cacagcttaa tttggatcgg gcttttcgca attatttcaa gggacgtgcc
    14881 agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat cttatacgac caacaaccaa
    14941 acccgtacga tctatctgga aaatggctac ttaaagttac caaaacaaaa agaactaatc
    15001 aagataaatc agcatcggcc agttgaagga tcgattcgct ccgctacgat ttctgctcga
    15061 tataatgaag aattttatgt cgcactactt tgtgatgttt cgccagtaaa aaaagaatct
    15121 ttagctaaat ggatcgggat cgcctatcat ccgaagacat tgatccaaac atctcgacct
    15181 ctagaagtaa ctttaccaaa atttcaccag acagaagaaa agctgcaaca tgcacagcgg
    15241 aaattgaatg tgaaagtccg ctccgctcac catagaaaga tcaggttaga tcaggcaagc
    15301 aattatcaga aacaaaagag aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga
    15361 gaagattatc ttgaacaatt atctgggaaa ttggtcaaac aatatgacta cttatttgta
    15421 gaatcttttc caaaagaaga ggcacatgct gatttttcta ttcacgactg gcataaatta
    15481 ataacaaaat tgcgttataa gtcccaatgg tataataaaa aatttctttt gatcaatacc
    15541 gacggagcag aagaaagcaa ttccgtaaga aagagccaag tagtagaaaa aatgggcaga
    15601 cattcggtta tcaagggata aaaaatgaat gaaactgata ggagcgttag ggttagcctt
    15661 gttaataaaa agtcttaatg acttttgttt ctaggaattt ttcttttgaa gaaaagctca
    15721 ctaattatga caaaccaata aaacaatagc agaaaaagtt cgtacgagct ttttctgcta
    15781 ttgttaattt tttattcaga aagtttacga atacatcaaa attctgaaac aaaaaacgta
    15841 actcacaaag agttgttttt gcgttttttc ttgaatgatt aggtctgaat cttttgctca
    15901 caaccttatt catggtgccc cggcttgagc tgatagattt ttgaaggacg gccgattcct
    15961 acaggacgtt caccgatctc atcgaaatat tggagcatag ctttcttaaa attggaatga
    16021 tcgatcgttt tataatcgat ccctaagaat ttagcaaata cttttcgagc ttccgtaatc
    16081 gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt gttccatttt attgactaca
    16141 cggttgaatg ctttcaagat gatttgacta tggtcaaaag ccaaagtatc tttgccagaa
    16201 gactctccag tgactaaatc aagcgagata gccacttctc cgctagataa atggatcttg
    16261 ttactatgac gttctagtgt gaaccagcgt acttccttgg catcatctcc ggcgattagc
    16321 ggttcttctc caataaaggc taagtagctg actgtgacga cccagcctcg cggatctcga
    16381 ttaggcgtac tgaatgtatg cagttgctca atattttctt ttgaaataac cacaccagtt
    16441 tcttcctttg tttctcttaa gacgctttct ccagttgact catccttttg gacaaagcca
    16501 ccaggcaatg cccatgaatt tcgataagga tgcccttttc tttggatcaa caaaactttc
    16561 agctgatctt cttctttgtt atagcacatc agtacgatat cgacagtcaa agagggcttg
    16621 tcatattctg gacgttcctg tttttgatac cagttaagaa attcttcttc acttgcttta
    16681 tgttcataat ataatttttc ttcttcttta gaagcaaaac ggttcatctg accacctcca
    16741 ttgacagtaa aagtactttt taccttattg tagcagactc tgaaaaaaaa ccaaggattt
    16801 acttgaaaaa tctaggttat catttaaata tgaaaaataa atggaagtat gagattgaat
    16861 aaaaagaaaa gcagataaaa cggcaatact ttataccgct cttcacgaat ggcaaagaag
    16921 ccacaaataa cgatatatac gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa
    16981 ctgttcaaat gcccatggag gttaaaataa ccatacatac gaatataaaa atagctgaat
    17041 attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc
    17101 atgattgttc cagtccctta ctaagataga tagtctttat tataccacgt gtgaggggag
    17161 aaataaaaag ccgtttttta gtagatatga aacaaaacaa aaacccgcat ttattcaacc
    17221 tattcatcct tttaaaggat agattgaacg tagatagaag tcgtttatcc agtctttttc
    17281 attttgaaaa aaagctaaca gcatgctttt tttatggtat actagcaagg aaacgaattc
    17341 gcatcatgcg ataaaggagg aagagccccg tggcagaaaa ggaaactttt tatatcacta
    17401 cgccaatcta ttatccaagc ggcaaacttc atatcggcaa ctcgtatact acgattgcat
    17461 gtgacgcaat tgctcgttat aaacggttga tgggctttga tgtgttttat ctcactggag
    17521 tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga actaggtgta aaaccgcaag
    17581 aatatgtaga taaaatggcg gctgatgtca agaaattatg gaaaacgctt gatatcagtt
    17641 atgataaatt cattcgcacg acagatgatt accataaagc agctgttcaa aaaatatttg
    17701 atcgtttatt agaacaggga gatatttact taggtgaata tgaaggctgg tactctgttt
    17761 ctgacgaaga attctttaca gaaacacaac ttgcagaagt gtacaaagat gacgaaggta
    17821 acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga attggtaaaa gaagaatcat
    17881 acttcttccg catgagcaaa tacgcagacc gtctattgga atactatgaa gaacatcccg
    17941 aattcatcca gccagaatct cgtaaaaacg aaatgatcaa caactttatc aaaccaggat
    18001 tagaggatct tgcagtttca cgaacaactt ttacttgggg agtaccagtc aagagtgatc
    18061 cgaaacatgt ggtgtatgta tggatcgatg cattatccaa ttatattact gcactaggat
    18121 atggctccga agatgaaagt ctattcgaaa aatactggcc tgcagatgtt cagatggtcg
    18181 gtaaagaaat cgttcgcttt catacgatct attggccaat catgctgatg gcccttgatt
    18241 tgcctctacc taaaaaagta tttgggcatg gctggctatt gatgaaagac ggaaaaatgt
    18301 ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt ggaacgttat ggtttagatg
    18361 cactccgtta ttatttgctt cgtgctgttc cgtttgggtc agatggtgtc tttacgccag
    18421 aagattttgt ttcccgtgtc aattatgatc tggcaaatga tctagggaac ttattgaatc
    18481 gaacgattgc tatgatcaac aaatattgtg atggactcgt tcctgactat gcctcacttg
    18541 tcactccatt tgatagtgaa ctgtctacga cggctgctaa cgttgtcgga cgttaccatg
    18601 atgcaatgga aaaaatggaa ttcaatacag cattggctga aatctgggtg ctgatttctc
    18661 gtgcaaacaa atacattgat gaaacagaac catgggtatt agcaaaagac gaagaaaaga
    18721 aagctgaatt gaatagtgtc atgatccatt tagctgaatc tttgcgtatc gcagcgatat
    18781 tacttcagcc aatcatgaca gaaacgccag agaagatctt caatcaacta ggattggact
    18841 ctgaaacaat gaatcttgaa ggattacact ttggcgaatt tccttcagga acaaaagtag
    18901 ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga agaagaagtt gcttttattc
    18961 aagaaaaaat gtctgaaggt actcaaacaa acgaagatac agtcaaatgg gatccagaag
    19021 aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag
    19081 aactaaaagt agcagaagtc atcaactgtc aaaaagtaga aggggcagat aagttgctgc
    19141 aattccgact agatgctgga gacagccaag accgtcaaat cttgtcagga atcgctgaat
    19201 tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt cattgtagca aatctgaaac
    19261 cacgaaagat gcgtggacaa atcagtcaag gaatgatcct ttctgcagaa gcaccagatg
    19321 gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa tggctcagaa atcgcataga
    19381 aattcaaagg gggtatgaca ctagttgtgt cataccccct ttttccgaaa tttgagaagc
    19441 aattttcgta aattccttct tattacttga agccaatcat ataatacaga aacagagaac
    19501 tatccaaaat aaatcctaga aaaagaagta cgagcttcgc tgatttttgt taatttagaa
    19561 taatactaat aggaatcgta aaaacagaaa aaaagcataa tgataaaggg tcgggcagat
    19621 tttaattgat tcatctggta ctctttttta ttgtccttac attcataaag tttgaatgca
    19681 atgactttat tacatctata tttatatcta acagtagcgt tgatgtgtta attataagtt
    19741 ctatcacttt atgttcaatt agtttgtgct cttatatgtg tgattgaaaa tatagataaa
    19801 aaaacaatca tcggctttga cttcgatgat tgttttcttt attttgtaaa aatcctatca
    19861 cttgatttta tggtaacctt ttatttttgt cttcaaacgg cttggtaaga gagcagtaag
    19921 cagtgcaaat gggatcgaaa agacatacca gaaaatcgta cgtccgatac cataatgttg
    19981 atagagcttt gtcaacatca cgtgtaacaa gatcaaccct aaaagcaagg agccagctat
    20041 aaaccaatga ttatcccaat gcatgacttc actaagtgcc atcactccaa aaggatagaa
    20101 ataaaaatag atcccgacta aggtcatccg ccagttcttt gtaaatgttc cgatattcaa
    20161 taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga
    20221 atacaacatc ccccacaaga tcatcacagg acctttgatc aatgcaacga ttgcgatgaa
    20281 caaagtcagc aatattagtt tttgtgataa cgttgcaaca gtcattaatc caaataaaat
    20341 taatgtgaac caatgccaat gggactgctg ttgttcttta cgggaaaaat cgacccggtt
    20401 ggataaataa tctgctaatt tcggataaga acgcttaaaa tccataaact gttcctcctt
    20461 cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga aatacatccg tcttaagagg
    20521 gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt aaaatattct cagttatact
    20581 gaaaattata ccaaatttca aagaagaaag aggatgggaa tgaaaggcaa actaaaacga
    20641 gaaatcaatc tgtttggcgc attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc
    20701 ttcttcaaaa ctgctgctgt tactgctagt actcagtcta caagtttgac attgctagct
    20761 tggcttttgg gtggtttctt aacgatctgt gctggattga cagtggctga attagctaca
    20821 gcaatcccgg aaactggtgg agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc
    20881 agctttttat taggatgggc gcaaagcttg atttactttc ctgctaatat tgctgcatta
    20941 tcgattatct ttgcgacaca attgacgaac ttattacaat tatctaccga ttatcttttg
    21001 ttgattgcag tgatcaccgc tgtttctgtg acagggttga atttgttagg gacaaaagtc
    21061 ggtgcttctg tacaatcagt gacgttgatt gttaaattaa taccaattgc tgtgattgtc
    21121 atatggggat tattgacccc aggacaagga actgtccagt tatttccaat cgaagctgga
    21181 aaagatgtta cttttgtcga aggattgagc agtgcgttgt tagcaacgtt gtttgcttat
    21241 gacggctggt taggtgtcgg tgcaatggca ggggaaatga aacggccgga aaaagatttg
    21301 cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg tttatctatt gatcaatttt
    21361 gtttttttga aaactttgcc gattgatcat cttgccggta atttgaatgc agcttctgaa
    21421 gcatcagacg tcatttttgg aggaatcggt ggaaagctgg tcactattgg gatcttgatc
    21481 tcggtatacg gtgcattgaa cggttataca ctaactggga tccgcgtgcc ttatgcgatg
    21541 gctttagaag acgagctacc tttcagtaaa cagctgacaa agctttcaaa gaagtttacg
    21601 gttccttatg ttcctgcggt tttccagtta gcagtagctt gtattatgat gagccttggt
    21661 tctttcgact ttttaacaga catgttgatt tttgtgatgt ggctattcag tttactgatt
    21721 tttatcggtg tctttatttt aagaaaaaaa gcgccggaat tgccacgtcc atataaagta
    21781 ccactttatc caatcgttcc gattattgcg attctcggtg ctattttcat tttaggtatg
    21841 acgatgatga cgcaaacgaa attagcattg atcggtatag gtgttacctt gataggtatc
    21901 ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt aaaatatttc aacaacagac
    21961 agaaaaagta cgaactaggt cctctagatc ttcgtgcttt ttctatttca ataaaacaag
    22021 ttcttctatt ttgatttttg ctacttctgc aagtagtgct tatgtgctaa agtaagaatg
    22081 aaaaggagag ataaacatga tatttgattc tcatacccat ttaaatgctg aacagttcaa
    22141 tgaagatatt ccagaaacga ttcagcgagc acaagaaatg ggggtaacga aaatggcggt
    22201 cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa ttaagccatg actatccaaa
    22261 tatatacagt attatcggct ggcatccgac agaggctggc agttatacga aagaaatcga
    22321 aaataaatta caagaacaat tgactttacc aaaggttgta gcattagggg aaattggctt
    22381 ggattatcat tggatggaag acccgaaaga agtccaagat aaagtatttc gcagacagat
    22441 tgctatcgca aaagaaatga acctaccaat cagtattcat actagagaag cacttgaaga
    22501 cacgtatcaa atcctaaaag aagaagatgt tcgagatatc ggtggaatca tgcatagttt
    22561 cagtggtgat tatgaatggg caaaacgatt tcttgactta gggatgcata tttcatttag
    22621 cggtgtcgtc actttcaaaa aagcattaga cgtccaagag gctgcagcaa atgtaccaat
    22681 cgaacgatta cttgtggaaa cagatgcccc ttatctggca ccagttcctt ttagaggaaa
    22741 gcgaaatgaa ccaggctata ctcgttatac agtagaaaag attgctgaac ttcggaatct
    22801 tccgacagaa gaagtggctg cccaaacatg gacaaatgcg caccgattat ttggtctaag
    22861 cgaacatgga taagatgaag atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa
    22921 gaaggctgcg ggaagtagtt gatgcagata cgatagaaac aatagggtct gcaatcaatg
    22981 aagagatcct gatgcaaatt gaacatgccc aagagactcg tggagtgatc gtttttaccg
    23041 atcctgattt ttcaggagaa aaaataagaa aaatcattat ggaagctgtt cctcaggcaa
    23101 aacatgcttt tttgccgcgc acacaagcta aaccaaagaa aaagggaagc cttggtgtcg
    23161 agcatgcaag tgatgctgct attttagaag cattgaaaaa ggtcgtgacg ccagacattt
    23221 cccaaacggc tgtttctgaa atcactagac aagacctttt agactatggt ttaatagctg
    23281 gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga gttgcgcatc ggttacacga
    23341 acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc
    23401 aagcaatgaa aatagtggag gaacaacatg agtgaatatc gtgaaattgc gacaccctct
    23461 agaacaaaag agattttatt gaaacatggt ttttcattta aaaaaagttt aggacaaaat
    23521 tttctgactg aaccaaatat tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat
    23581 acgaacgtga tcgaagttgg accaggaatc ggtgcattga cggaacaatt agccaagcat
    23641 gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga tccctgtatt ggctgatacg
    23701 atgcaaccct atgacaatgt gactattgta catcaagata ttttaaagac cgatttgtca
    23761 acagctgtta gagaaacatt tcatgaggag cttccgttaa aagtcgtagc gaatttacct
    23821 tactatatta cgacaccgat catgatgcat tttcttgaat cagatttggt tgtggatgaa
    23881 ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct ctgctgaacc aggaacgaaa
    23941 gcttatggtt cattatcgat tgctgttcaa tattatatgg aagccagttt agcattcatc
    24001 gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt cagcgatttt gaaactgaca
    24061 agaagagata taccggcagt cgaagtgaca gacgaaaaag catttttccg attgacaaaa
    24121 gcggcatttc agcaaagaag aaaaacgctt tggaataact tgcagcattc gtatggcaaa
    24181 gatgaccaga caaaagcatg gttagcaaaa agcttggaaa cagctggaat cgatccaaaa
    24241 cgtcgtgggg agactttgtc attacaagaa ttcgccgcat taagcaatgc gatgtcagaa
    24301 aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt
    24361 aataagaaca gccaaacaaa aatagttcct catgtttttc gtttgtttgg gcttattacc
    24421 gcagatgcaa gcttttgaac accgtttaag aaagaggttg tgggcctgcc gtttaactct
    24481 aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tttgagttag ttggtcttat
    24541 tacttgatgc aagcttttga ccactattat gccgaacaag gagatgtgac acaaattttg
    24601 tcacatctcc tatttttgtt tcttgatcaa aaaattctta tacttgatac tcacactcat
    24661 tttgtatgga tttagaaaag aatatgtgtc ttgacagtta ttagtaagtg agttatacta
    24721 agtcgacatt tatgtgtcaa gacatattag gagagagaaa aaatgagaaa aaattcagtt
    24781 cgaaaactaa cgatttctgc tttattgatt gggatggggg tcattatccc aatggtcatg
    24841 cctaaaatca tgattccacc agcttccttt actttggcgt ctcatgtgcc attatttatt
    24901 gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc taggtacgac atttggtttc
    24961 tttttaacaa cgccggttat catcgcattg cgagcattgt cacatcttgt gtttgcgttg
    25021 attggggctt ggtacttgca aaagcatccg agtatcgttt taaaaaatgg acagtttact
    25081 tttgctaatt ggcgtttcca agggttcaat tttgtgatcg gtgtgattca ttctttagca
    25141 gaaatgctag tagtcagcat cttttacttt attggtaata tgcctgaaac atattattct
    25201 caaggatatt tttatacagt attcatcttg atggggattg gtgggcttat ccacagttta
    25261 gttgattata atattgctta ttttatagca ggaacactca gcaaatattt tgatatcccc
    25321 gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa
    25381 acgacatatt agacatgatc tctcaaaaaa aaaactctca tctagtaatg gataataaat
    25441 cctctaagat gagagttttt tgtcctaaaa agagctgcac caacatcttc tggcacagct
    25501 ctaacagggg gcaatttatg gaagtttgat gacttgttct gtttctttat caaagaagtg
    25561 gcctttgctg atgttgaagg caaggtcgac tatttcgcct ggtttatgga aatctcttgc
    25621 atctactttt gagatgaact cagtagaacc agtttttgtg taaagcatcg tttcagctcc
    25681 aagtaattcg gaaacgacaa cttctgattt cactacggct tcaggtgaag cttcaagggc
    25741 tacttgttca ctgtggatgt cttctggacg aataccgaag ataacttctt ttccttcata
    25801 acctttttca actaatactt tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc
    25861 atctgtgata acaccatttt tcaatgtcac attaaagaag ttcatggcag gagatccaat
    25921 aaagccggca acgaacatat tgacgggtgt atcataaact tctttcggtg taccgatttg
    25981 ttggataaat ccgtctttca tgataacgat tcggtcagcc atagtcattg cttctgtctg
    26041 atcgtgagta acatagatgg tcgttgtttc aagacgctgg tgaagtttgg caatctcagc
    26101 acgcatggct acacgtaatt tagcatctaa atttgataat ggctcatcca ttaagaagac
    26161 ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc caccagataa
    26221 agcagctggt tttcgtttca agtattctgt cagacctaag atctctgctg cattgtccac
    26281 acgttttttg atgtctgctt tatcatattt acgtaatttc aagccaaaag ccatattatc
    26341 aaaaacagtc atatgaggat acaatgcgta gttttggaaa accatggcaa tatcacggtc
    26401 tttaggtgcc acgtcattca tgacttttcc gccgatattt agttctcctt cagagatatc
    26461 ttccagtccc gcgatcattc gcaaagtagt tgactttcca caaccggaag gaccaacaaa
    26521 aacgataaat tcacggtctt tgattgatag attaaaatca gttacagaat aattttctgc
    26581 attgtcatat tttttatata cgtttttcag agccatttct accataatac gttccacact
    26641 ttctttttta gttttattaa ctatttaaag tataaatgaa agcgttaact aacttcaatg
    26701 tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg ctagttttaa gctttaataa
    26761 atatccataa gaaataaaaa ctgatacact ggagtaaata agtatgttaa caatatcaaa
    26821 aggagtgtct cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt
    26881 gaaattagca acagcgtata aaacaatatt ggaaaaacat gaactttttg ctaccgggac
    26941 gactgggcag aggatcacgg aagcaaccgg cttgccgatc catcggttta aatccggacc
    27001 gttaggcggg gatcagcaaa ttggagccat gatttcggag gatcagttag atttggtgat
    27061 ttttcttcga gacccgctgg ctgcacagcc ccatgaacca gatgtaacag cacttatccg
    27121 tttatctgat gtctatgaga ttccgttagc tacgaatatc ggaacagctg aggtgctact
    27181 aagaggactg gaagccggct tcctagattt tcgaagaatc gtccatgaaa tagacaagca
    27241 tccactttca ttttgatgga taagcgtatg gaaaaataac gtttaatgtt cgcctatatg
    27301 tagctataaa agctaaacaa actgataata tgcgatataa tacgaacgta ttggataata
    27361 taaacaataa tattagtttt tcgtatttgc agtaaatgtg aaaatatgct atagtttctg
    27421 tgggaaaaga tcccaaaaat aatacagagt agaaaataaa gcgttaagtg ctaagaggat
    27481 gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt tttattttcg cattcgctgc
    27541 atgatacgat gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac cggttagacg
    27601 cacgtatgat tgctattatg ggcttattga ttgcattgat ggtcactttg tcccgactgg
    27661 tagcaatcga gaccccattt attaaaatca gtgtcacatt tattccacaa gtgatcatgg
    27721 gcattctttt tggtcctttt tggtcaggga taggcgcagt tttagctgat ttagtaggaa
    27781 tggccctctt ctcgaaatct gcattcttta ttggatttac attgaatgca tttattgaag
    27841 gagcaatcta cggattcttt ttctatcgaa aagagatcac ttggaaaaat gccattctgg
    27901 ctacgcttag tgtcacgctg atcatcaact tatttttgac accattatgg cttgcgttga
    27961 tgtaccatgt gccattgttc agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa
    28021 tatggttacc gattcaatca attgccattt attacgtagg acgtagtatt ccttacaaaa
    28081 aaattttaag aagtttagcg atccacgcta aataaaaaac aagcagtctt cccatcccag
    28141 aagactgctt gtttttattg tgctgtgaat tgtgcttgat agagccgtcg gtaataacca
    28201 tctttttgaa gcagctcatc atgggtgccc atttcgacaa tagctccctg atccatcact
    28261 aatatctggt ccgcattttt gattgtggac aggcgatggg caatcacaaa actggttttt
    28321 ccttccatca ttgctaagaa ggcttgttgg atttttttct ctgtcaatgt atcgactgag
    28381 ctggtagctt catctaagat caacataggg ggattactga tcatcgttcg agcaatcgtc
    28441 aacagttggc gttgtccatc tgatatcttt aacccttgac cgccaatctt agtatctaat
    28501 ttttccggca aacgttctac aaattcatac atataagagg cttttagggc atcattgatt
    28561 tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat
    28621 agccaagtat cttgtaacac cattccaaaa cttttacgta atgtatcacg ttggatagag
    28681 cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc
    28741 aaattaacaa gtgtggattt tccggcccct gtttttccta ctatagcaat ggtttgacct
    28801 ggataagcag taaaattgaa atcttcgatc aaacgctgct tggggtcata agaaaaagaa
    28861 acatgatcaa aagagacttt tcctttgata tttttgggtt ccaaggcata ggcatctggc
    28921 aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa
    28981 gcggtttgca gttgtgtcgt gattccagaa agttcgataa atggtttaga aaactgactt
    29041 gagtagatcg taaagctgga aatcacacca acagtgattg acgaaccact cttcaatgcc
    29101 aataggccgc caactaaacc aatcgataaa taagcaaggt gatctacaaa acgagaagac
    29161 gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt tttcatatag atcagcattt
    29221 agcagttcaa attgctgttg actagcttct tcttgctgaa aagctttcac gattttctgg
    29281 ttaccgattc gttcggtcac aaagcccgag atatccccaa taatcctttg ctgtctatcg
    29341 aaatttgttt gtgaagcacg agcaacgatc caactcacaa ggaaaataat tggtgtagaa
    29401 caaagaacga ccaatgtcag aagtggactt agacgcatca taaaaataaa cgaaagaatg
    29461 atgattgcca ctccagaaaa taattgattg aaggccgcag aaattgcaat ggaaatattg
    29521 tccatatcat ttgtaaatcg actgacgata tttccgtggg agttttgatc ataataacga
    29581 agtggcaatc gattcagatg agcaaatgca tcttttctta attttgcaac ggataagtaa
    29641 gctaaccgat tgcttaacac ttggatgagc cattgactga ctacgttgat taaaacgata
    29701 ccaccgaata gaataagtaa atggaacagt cgattgaagc ggacctgtcc ttctccaatc
    29761 atttgatcga tactttgacc aatttgtaac gtcatcacaa ctgttgcaat cccattgatg
    29821 attcctagta tcacagctag ccaaatgtct tttgcatagg cagtaaggta aggaatgaat
    29881 cggcggagcg tctgcaagga taattttttt actttcataa ctattcttcc tcctgtgatg
    29941 cgacgatttc ctgatattct gctgattgag caagtaattc ttcatgggta ccctgagcag
    30001 caatcttacc ttgatccatc actaagatat gatgtgcttc ttgaatagag cggacacgtt
    30061 gggagataat aatcactgta ctgccaagat aagtctgtag cgccttacgc aaatccaagt
    30121 ctgtttgata atccaacgca cttaaagagt catctaaaat cagtaatgct ggcttggcaa
    30181 tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc agagaaattt ttcccatttt
    30241 ccatgacttt tgtatctaaa ccatttggaa gctgacggac aaattcttct gcctgagcga
    30301 ttttcagagc ttcccaacat tctttttctg tggcgtcttt cttaccccat tgaaggtttt
    30361 cacgaatagt cccggtaaat agtacagaag tttggggaac aagagcaatt tgctgacgga
    30421 gatgatccag ctgccaagaa cgaacatcca ttccgtccat tataactgta ccggaactga
    30481 catcatagaa acgcggaatc aattgtgtca aagtcgtttt cccgcttccg gttggaccaa
    30541 tgattccaag gactgtattt cgcttcagct gaaatgtaat attttggaga gaaagaccag
    30601 aatcaggagt ataacggaag tcaacatggt caaaagcgat aattgcttca gaagaaaaat
    30661 cgggagtagt actgacctca gcttcaagaa tcgaattttc tgtgtctagc acctctttca
    30721 cccgatttcc tgaagcttcg gcacgtgtga agatcacgac gaggtttgaa acaacgatca
    30781 gtgccaaaag catttgattc atataattga tcaaagcaag aacttgtcct tgttgcaagc
    30841 taccaatatt tactttgatc ccgccaactg tcagcaaaca gatgatcccg acattcatga
    30901 tcaaggttgt agcaggtgat aatagggcag agatattgga aacacgtaaa taattgttcg
    30961 ctagatcatt tgtgctttta tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc
    31021 ggatgacacg aacaccgcta agattctgac taacgtgttc attgagctga tctagttttt
    31081 cttgcacttt ttggtagaga ggaacggttt ttttgatgat aaaaaataaa atgattgaga
    31141 aaataggcaa taatgctaag aaaaacagac ctacttccca gtcgatcaca aaagccatga
    31201 ccactgaccc gatacttaaa aacggtgctc tgatcaataa acgaatgacc attgctaaag
    31261 ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat caaggtatct gttccaaaat
    31321 gattcaactc gttgtgagag aaagtattga tttttttgat tagctgatta cgtaactctg
    31381 taccaaatcc ttgtgaagct actgaagcat agtattggca aatgatagca caaatcagac
    31441 caatcacaga catagcgacc atccataagc ccatttcaat gatttttcct cggtcattca
    31501 tttttagtcc attatcgatc aaggaagcca tcattaaagg caggacaagt tcgaagacag
    31561 cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt acgatagtct tttgcataac
    31621 gtaataactg aatcatatga ttcctcctat tatttatttg tccacaattg aaaaaacaag
    31681 tactaccaca ttgtagctta ggaaatttag aatgaaaaga gaacaagcct tttcataaat
    31741 gaaagtttta ttatgaagaa gctttagaaa ttgaatagac tatttttttt aaaaaaaaga
    31801 aaatgagaaa acatgccagt ctcttcattc ataggaacgt ctattttact taatttttct
    31861 ataaatgaat tctgcataaa aaatatatca agtaaatgtt tatagatgaa actctattta
    31921 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
    31981 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
    32041 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
    32101 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
    32161 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
    32221 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
    32281 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
    32341 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
    32401 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
    32461 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
    32521 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
    32581 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
    32641 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
    32701 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
    32761 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
    32821 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
    32881 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
    32941 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
    33001 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
    33061 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
    33121 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
    33181 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
    33241 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
    33301 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
    33361 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
    33421 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
    33481 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
    33541 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
    33601 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
    33661 atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
    33721 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
    33781 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
    33841 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
    33901 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
    33961 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
    34021 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
    34081 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
    34141 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
    34201 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
    34261 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
    34321 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
    34381 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
    34441 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
    34501 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
    34561 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
    34621 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
    34681 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
    34741 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
    34801 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
    34861 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
    34921 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
    34981 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
    35041 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
    35101 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
    35161 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
    35221 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
    35281 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
    35341 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
    35401 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
    35461 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
    35521 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
    35581 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat
    35641 gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa
    35701 ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct
    35761 atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag
    35821 cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg
    35881 atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct
    35941 atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg
    36001 aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt
    36061 tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat
    36121 gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac
    36181 acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat
    36241 acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat
    36301 tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt
    36361 agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat
    36421 caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat
    36481 tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat
    36541 ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa
    36601 cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat
    36661 atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg
    36721 tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg
    36781 gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga
    36841 cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga
    36901 ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga
    36961 accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact
    37021 tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg
    37081 tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt
    37141 tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga
    37201 tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct tacttagaga
    37261 ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta
    37321 tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat
    37381 tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct
    37441 tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca
    37501 atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa
    37561 cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg
    37621 gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt
    37681 atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact
    37741 ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc
    37801 cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca
    37861 ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg
    37921 gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc
    37981 acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt
    38041 caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg
    38101 ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt
    38161 ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat
    38221 gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt
    38281 ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc
    38341 ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga
    38401 tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt
    38461 gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca
    38521 aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga
    38581 agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg
    38641 ctattctgcg actgttttac agttactact cttagggata ggaattggaa taagtaaaaa
    38701 taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca
    38761 tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg
    38821 tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc
    38881 agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta
    38941 tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga
    39001 aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga
    39061 gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga
    39121 atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa
    39181 aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt
    39241 cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat
    39301 caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact
    39361 ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc
    39421 gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc
    39481 tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc
    39541 ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg
    39601 agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt
    39661 tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg
    39721 attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg
    39781 ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt
    39841 tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg
    39901 agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc
    39961 tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt
    40021 cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt
    40081 gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat
    40141 cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg
    40201 catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg
    40261 tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag agagcagcga
    40321 taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt
    40381 tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt
    40441 tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca
    40501 acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc
    40561 ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg
    40621 ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc
    40681 taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca
    40741 taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa
    40801 aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa
    40861 atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa
    40921 atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac
    40981 gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat
    41041 catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa
    41101 gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg
    41161 attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat
    41221 gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa
    41281 attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa
    41341 gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc
    41401 tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt
    41461 ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat
    41521 ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc
    41581 agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg
    41641 ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg
    41701 ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt
    41761 gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt
    41821 actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa
    41881 atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa
    41941 tagttaatcg tcgtttagga ggaacagcaa gtgaaggtca aaaaagcagt cattcctgcc
    42001 gctgggctag gaacacgttt tttgccggca acaaaagcca tggcaaaaga aatgcttcca
    42061 atcgtggata aaccaacgat ccagtttatt gtggaagaag cattggattc gggaatcgaa
    42121 gacatcttga tcgtaacggg aaaagaaaaa agacccattg aagatcactt tgatgcaaat
    42181 atcgaattgg aaaacaattt gcgggaaaag gaaaaaacag aattactcgc tttagttgaa
    42241 gaaacgactc aagtcaatct tcacttcatc cgtcaatctc atccaagagg actgggggat
    42301 gccgtgcttc aagcaaaagc atttatagga aatgaaccgt ttgtcgtcat gctgggggat
    42361 gatttgatga aagatgatat cccattgact caacaattga tgatggatta tgaagaaaca
    42421 caagcttccg ctgttgccgt catgcgtgta ccagaaaatg aaacatctaa atacggaatt
    42481 attgatccta aaacagatag cgaaaagggt cgttgcagag taaaagggtt tgtagaaaaa
    42541 cctgaaatgg gaaaagcacc aagtaattta gccattatcg gacgttatct actgactcct
    42601 aaaatatttg agatattaga aactcaagaa ccaggagcag gaaacgagat acagctaacg
    42661 gatgcacttc agactcttaa ccagacagag gcggtctatg cgagagagtt taaaggaaaa
    42721 agatacgacg taggcgataa gttaggatat atgaagacaa atattgaata tggacttcaa
    42781 cattcggaaa ttgggacttc attaagtgca tatattatcc agttgagtaa aaagctaaga
    42841 acagaaagtt aactggaaat aagtggcctg ctgagaatga aaggctattt gttattaaag
    42901 cggcagcttg tgcgtcattt gcgcaactgt cgttttagtt ttttgactct agatcagttg
    42961 agtcgtggaa aacgaaagat agaaaaagac tttcgtgaat agattcagaa aatattatgc
    43021 taatggttta attttttttt aagttgtata aaagaaaatg attacataag agaaatgaaa
    43081 gccaagccag aagattcata gtagaataaa aaggcgaatt ttttaattat aaaaaaaggt
    43141 gtgtgttcac tatgggaaaa ttagtcgctg ttgcacagtc gctgcttgat atactcaagt
    43201 ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt agtctattta
    43261 tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg tatgcagcat
    43321 ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa gcttgcgtat
    43381 ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg aaaagaaagt
    43441 ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat tctctcgaac
    43501 aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa aaaagataaa
    43561 ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa gcttttttca
    43621 cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag ccattccttt
    43681 ttttatgtta tttatatata tataaatcat accaatgttt ttttgtattt tcttgcttgc
    43741 acttggataa gagattactt acaataaatt cagatagaaa cagctgaata aaaaaaggct
    43801 ccctgaccct tagttggcgc taagaatcag ggaatgacca gtcacacaca tacaccctga
    43861 ccgatcattt tgccatagtc aatgcagtgc atcgacctaa tttattgtac ctaatctagg
    43921 gtgaaaattc tacccctatg tagatatttt ccatggtctg cacgttgttt tatttgctat
    43981 gtcaaggtat tgggcgtatt cgttcaatgc cttttttgtg tttgaaacag caaagaccac
    44041 atgtgttact gtctaactac tgttttttta tgtacttcct tagattaggc agctgtttct
    44101 attcatgtca agtatagcta ggcgggagag ataaatgagt ttatgttatt ttgactatta
    44161 tcaccaattg gaagagaaat taaggataga agagccgctg atgcttcaag ctggcacttg
    44221 gatcgcgaaa caaattaatc aaggtggtag attgcagata tatgctagcc gcacactaag
    44281 tggtgtagca tttgaatttt gggatcaaac cccggatctt gtaccaagta tattgattga
    44341 aaatccagcc ggaggtgtct atgaaacgct agaaggtgca ggacaggcaa ttattgatca
    44401 gctttctgtc aaatcagaag acatttttct tcttctttcg aatgaaggaa gaaacccggc
    44461 aatcatcgaa ctagctcagt ggatcaaaga aaatggtcat ccattaatca ttgttactgg
    44521 atttgatttg tcacgaagta tcaaatcgca acatagcagt ggtttgcgat tatttgaatt
    44581 tgctgatttg gttttggaca accacgccaa tttacatgat gcagtcttga ctttgccaaa
    44641 tctagatcca gcgatctgcg gaacagcctc tcttgcaacg atctttttgc tgcagcaagt
    44701 tttgtatttt acagcaaaac agctagtcca ggacacaagt ggaaaatagt tatccacatg
    44761 ttgacaaagt tccttaaaaa aataatgttc cacataaaaa tggagcgttt gaaaaaaaga
    44821 aaacgattat taatgaggat taacaacgac tacttttttt gttttagtta ttttaagatg
    44881 gatcatcttt gattttaagc ttgttttgtt taaagtttgt tatttttata agttgtttca
    44941 cagttatgag aaatgagtta taagaatatg tagtgtcaga aaaataaaaa gtagtggaaa
    45001 aagagacaag aaagaatcca tatggattct ttcttgtctc ttaatacacg ttgatgaaag
    45061 agtcttcccc aaaactcttt acaaagtaat tatagtacat ttgttatcat tattgcaatg
    45121 aagtttacct gatataacaa aattgtccaa caggatacct ttttctaata gaaataaaat
    45181 acacaaaaat tgaaggatag ataatggata tttataagtg tttattattt tttttgcaat
    45241 gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct
    45301 aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa
    45361 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
    45421 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
    45481 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
    45541 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
    45601 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
    45661 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
    45721 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
    45781 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
    45841 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
    45901 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
    45961 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
    46021 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
    46081 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
    46141 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
    46201 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
    46261 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
    46321 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
    46381 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
    46441 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
    46501 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
    46561 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
    46621 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
    46681 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
    46741 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
    46801 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
    46861 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
    46921 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
    46981 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
    47041 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
    47101 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
    47161 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
    47221 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
    47281 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
    47341 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
    47401 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
    47461 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
    47521 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
    47581 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
    47641 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
    47701 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
    47761 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
    47821 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
    47881 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
    47941 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
    48001 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
    48061 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
    48121 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
    48181 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
    48241 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
    48301 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
    48361 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
    48421 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
    48481 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
    48541 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
    48601 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
    48661 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
    48721 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
    48781 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
    48841 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
    48901 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
    48961 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
    49021 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
    49081 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
    49141 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
    49201 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
    49261 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
    49321 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
    49381 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
    49441 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
    49501 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
    49561 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
    49621 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
    49681 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
    49741 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
    49801 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
    49861 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
    49921 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
    49981 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
    50041 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
    50101 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
    50161 agtatgataa taccggctac agtcactagt aaaacaacat ttttagaaaa acttgccacg
    50221 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
    50281 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
    50341 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
    50401 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
    50461 ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
    50521 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
    50581 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
    50641 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
    50701 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
    50761 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
    50821 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
    50881 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
    50941 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
    51001 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
    51061 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
    51121 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
    51181 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
    51241 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
    51301 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
    51361 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
    51421 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
    51481 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
    51541 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
    51601 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
    51661 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
    51721 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
    51781 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
    51841 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
    51901 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
    51961 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
    52021 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
    52081 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
    52141 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
    52201 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
    52261 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
    52321 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
    52381 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
    52441 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
    52501 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
    52561 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
    52621 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
    52681 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
    52741 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
    52801 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
    52861 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
    52921 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
    52981 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
    53041 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
    53101 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
    53161 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
    53221 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
    53281 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
    53341 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
    53401 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
    53461 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
    53521 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
    53581 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
    53641 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
    53701 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
    53761 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
    53821 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
    53881 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
    53941 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
    54001 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
    54061 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
    54121 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
    54181 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
    54241 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
    54301 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
    54361 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
    54421 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
    54481 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
    54541 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
    54601 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
    54661 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
    54721 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
    54781 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
    54841 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
    54901 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
    54961 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
    55021 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
    55081 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
    55141 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
    55201 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
    55261 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
    55321 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    55381 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    55441 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    55501 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    55561 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    55621 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    55681 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    55741 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    55801 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    55861 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    55921 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    55981 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    56041 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    56101 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    56161 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    56221 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    56281 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    56341 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    56401 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    56461 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    56521 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    56581 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    56641 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    56701 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    56761 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    56821 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    56881 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    56941 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    57001 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    57061 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    57121 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    57181 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    57241 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    57301 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    57361 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    57421 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    57481 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    57541 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    57601 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    57661 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    57721 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    57781 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    57841 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    57901 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    57961 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    58021 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    58081 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    58141 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    58201 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    58261 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    58321 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    58381 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    58441 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    58501 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaa
//
LOCUS       DACERL010000010        56785 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.010, whole genome shotgun sequence.
ACCESSION   DACERL010000010 DACERL010000000
VERSION     DACERL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56785
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.010"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(105..629)
                     /locus_tag="IXF54_000595"
     CDS             complement(105..629)
                     /locus_tag="IXF54_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403880.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXF54_000596"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXF54_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1403881.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXF54_000597"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXF54_000597"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1403882.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IXF54_000598"
     CDS             complement(2351..3031)
                     /locus_tag="IXF54_000598"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1403883.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IXF54_000599"
     CDS             3157..4026
                     /locus_tag="IXF54_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1403884.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IXF54_000600"
     CDS             4052..4567
                     /locus_tag="IXF54_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403885.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IXF54_000601"
     CDS             complement(4614..5177)
                     /locus_tag="IXF54_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1403886.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IXF54_000602"
     CDS             complement(5232..5411)
                     /locus_tag="IXF54_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403887.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IXF54_000603"
     CDS             complement(5488..5997)
                     /locus_tag="IXF54_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403888.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IXF54_000604"
     CDS             complement(6093..6473)
                     /locus_tag="IXF54_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1403889.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IXF54_000605"
     CDS             6803..7552
                     /locus_tag="IXF54_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1403890.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IXF54_000606"
     CDS             complement(7736..9013)
                     /locus_tag="IXF54_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403891.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXF54_000607"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXF54_000607"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1403892.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IXF54_000608"
     CDS             complement(9935..10879)
                     /locus_tag="IXF54_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1403893.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IXF54_000609"
     CDS             complement(10881..11567)
                     /locus_tag="IXF54_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1403894.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IXF54_000610"
     CDS             12041..12361
                     /locus_tag="IXF54_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1403895.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IXF54_000611"
     CDS             12515..13420
                     /locus_tag="IXF54_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403896.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IXF54_000612"
     CDS             13417..14190
                     /locus_tag="IXF54_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1403897.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IXF54_000613"
     CDS             complement(14371..15795)
                     /locus_tag="IXF54_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1403898.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IXF54_000614"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IXF54_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1403899.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IXF54_000615"
     CDS             complement(16517..18499)
                     /locus_tag="IXF54_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1403900.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXF54_000616"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXF54_000616"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1403901.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IXF54_000617"
     CDS             complement(19412..20473)
                     /locus_tag="IXF54_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1403902.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IXF54_000618"
     CDS             complement(20684..21211)
                     /locus_tag="IXF54_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1403903.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IXF54_000619"
     CDS             complement(21278..22780)
                     /locus_tag="IXF54_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1403904.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IXF54_000620"
     CDS             complement(23038..24159)
                     /locus_tag="IXF54_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1403905.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IXF54_000621"
     CDS             24285..25226
                     /locus_tag="IXF54_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1403906.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IXF54_000622"
     CDS             complement(25376..26272)
                     /locus_tag="IXF54_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1403907.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IXF54_000623"
     CDS             26541..27881
                     /locus_tag="IXF54_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1403908.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IXF54_000624"
     CDS             28099..28542
                     /locus_tag="IXF54_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1403909.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IXF54_000625"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IXF54_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1403910.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IXF54_000626"
     CDS             complement(30867..32090)
                     /locus_tag="IXF54_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1403911.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IXF54_000627"
     CDS             complement(32121..32960)
                     /locus_tag="IXF54_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1403912.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IXF54_000628"
     CDS             complement(32977..33657)
                     /locus_tag="IXF54_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403913.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IXF54_000629"
     CDS             complement(33659..34732)
                     /locus_tag="IXF54_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1403914.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IXF54_000630"
     CDS             complement(35379..36050)
                     /locus_tag="IXF54_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1403915.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IXF54_000631"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IXF54_000631"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1403916.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IXF54_000632"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IXF54_000632"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1403917.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IXF54_000633"
     CDS             complement(39933..40799)
                     /locus_tag="IXF54_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1403918.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IXF54_000634"
     CDS             complement(40959..43052)
                     /locus_tag="IXF54_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1403919.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="IXF54_000635"
     CDS             complement(43801..44424)
                     /locus_tag="IXF54_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403920.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="IXF54_000636"
     CDS             complement(44583..45980)
                     /locus_tag="IXF54_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1403921.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="IXF54_000637"
     CDS             complement(46012..46983)
                     /locus_tag="IXF54_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1403922.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="IXF54_000638"
     CDS             47357..47782
                     /locus_tag="IXF54_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403923.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="IXF54_000639"
     CDS             complement(48270..48971)
                     /locus_tag="IXF54_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1403924.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="IXF54_000640"
     CDS             complement(48987..49427)
                     /locus_tag="IXF54_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1403925.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="IXF54_000641"
     CDS             complement(49427..50002)
                     /locus_tag="IXF54_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1403926.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50850..51329)
                     /locus_tag="IXF54_000642"
                     /pseudo
     CDS             complement(50850..51329)
                     /locus_tag="IXF54_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51488..52189)
                     /locus_tag="IXF54_000643"
     CDS             complement(51488..52189)
                     /locus_tag="IXF54_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1403927.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52297..53706)
                     /locus_tag="IXF54_000644"
     CDS             complement(52297..53706)
                     /locus_tag="IXF54_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1403928.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53754..55619)
                     /locus_tag="IXF54_000645"
     CDS             complement(53754..55619)
                     /locus_tag="IXF54_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1403929.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55740..56591)
                     /locus_tag="IXF54_000646"
     CDS             complement(55740..56591)
                     /locus_tag="IXF54_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1403930.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT        16367 a        11970 c         9854 g        18594 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcata acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgcggg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagataa tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
    50101 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
    50161 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
    50221 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
    50281 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
    50341 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
    50401 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
    50461 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
    50521 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
    50581 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
    50641 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
    50701 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
    50761 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
    50821 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
    50881 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
    50941 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
    51001 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
    51061 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
    51121 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
    51181 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
    51241 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
    51301 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
    51361 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
    51421 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    51481 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
    51541 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
    51601 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
    51661 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
    51721 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
    51781 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
    51841 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
    51901 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
    51961 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
    52021 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
    52081 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
    52141 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
    52201 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
    52261 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
    52321 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
    52381 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
    52441 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
    52501 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
    52561 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
    52621 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
    52681 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
    52741 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
    52801 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
    52861 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
    52921 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
    52981 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
    53041 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
    53101 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
    53161 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
    53221 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
    53281 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
    53341 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
    53401 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
    53461 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
    53521 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
    53581 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
    53641 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
    53701 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
    53761 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
    53821 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
    53881 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
    53941 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
    54001 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
    54061 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
    54121 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
    54181 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttctcctgc attcgcatgt
    54241 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
    54301 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
    54361 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
    54421 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
    54481 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
    54541 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
    54601 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
    54661 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
    54721 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
    54781 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
    54841 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
    54901 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
    54961 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
    55021 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
    55081 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
    55141 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
    55201 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
    55261 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
    55321 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
    55381 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
    55441 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
    55501 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
    55561 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
    55621 gttttttcct tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca
    55681 caaacattcg tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct
    55741 tatgattgga tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc
    55801 tgctctgcga tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg
    55861 acacaatgaa acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc
    55921 tcgtcagatc cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata
    55981 cgctgataat tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc
    56041 agtatatctc ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt
    56101 tggctattca caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc
    56161 gtcttttgtt tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat
    56221 ttttttgttt caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc
    56281 gcataatgaa gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt
    56341 ttatcttcag ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca
    56401 gccatcagct gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg
    56461 accgcttctg gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata
    56521 caaacgactt cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta
    56581 tcaattttca ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact
    56641 gtttcttagc tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa
    56701 atcatcatac catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact
    56761 ggaacacaag tctatctaga aaaca
//
LOCUS       DACERL010000011        53717 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.011, whole genome shotgun sequence.
ACCESSION   DACERL010000011 DACERL010000000
VERSION     DACERL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53717
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.011"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            159..839
                     /locus_tag="IXF54_000647"
     CDS             159..839
                     /locus_tag="IXF54_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1403931.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            929..1963
                     /locus_tag="IXF54_000648"
     CDS             929..1963
                     /locus_tag="IXF54_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1403932.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1977..2312
                     /locus_tag="IXF54_000649"
     CDS             1977..2312
                     /locus_tag="IXF54_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403933.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2296..4776
                     /locus_tag="IXF54_000650"
     CDS             2296..4776
                     /locus_tag="IXF54_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403934.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4832..6391
                     /locus_tag="IXF54_000651"
     CDS             4832..6391
                     /locus_tag="IXF54_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1403935.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6388..7752
                     /locus_tag="IXF54_000652"
     CDS             6388..7752
                     /locus_tag="IXF54_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1403936.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7923..8993
                     /locus_tag="IXF54_000653"
     CDS             7923..8993
                     /locus_tag="IXF54_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1403937.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9008..9676
                     /locus_tag="IXF54_000654"
     CDS             9008..9676
                     /locus_tag="IXF54_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403938.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9696..10274
                     /locus_tag="IXF54_000655"
     CDS             9696..10274
                     /locus_tag="IXF54_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1403939.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10675..11586
                     /locus_tag="IXF54_000656"
     CDS             10675..11586
                     /locus_tag="IXF54_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1403940.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11964..12881)
                     /locus_tag="IXF54_000657"
     CDS             complement(11964..12881)
                     /locus_tag="IXF54_000657"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1403941.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13083..13520
                     /gene="zapA"
                     /locus_tag="IXF54_000658"
     CDS             13083..13520
                     /gene="zapA"
                     /locus_tag="IXF54_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1403942.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13578..14126
                     /locus_tag="IXF54_000659"
     CDS             13578..14126
                     /locus_tag="IXF54_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1403943.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14241..16601
                     /locus_tag="IXF54_000660"
     CDS             14241..16601
                     /locus_tag="IXF54_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1403944.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16688..17002
                     /gene="trxA"
                     /locus_tag="IXF54_000661"
     CDS             16688..17002
                     /gene="trxA"
                     /locus_tag="IXF54_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1403945.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17184..18962
                     /gene="uvrC"
                     /locus_tag="IXF54_000662"
     CDS             17184..18962
                     /gene="uvrC"
                     /locus_tag="IXF54_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1403946.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19107..20006
                     /locus_tag="IXF54_000663"
     CDS             19107..20006
                     /locus_tag="IXF54_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403947.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19999..21262
                     /locus_tag="IXF54_000664"
                     /pseudo
     CDS             19999..21262
                     /locus_tag="IXF54_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            21423..23321
                     /locus_tag="IXF54_000665"
     CDS             21423..23321
                     /locus_tag="IXF54_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1403948.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IXF54_000666"
     CDS             complement(23651..24370)
                     /locus_tag="IXF54_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1403949.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IXF54_000667"
     CDS             24707..25039
                     /locus_tag="IXF54_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403950.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IXF54_000668"
     CDS             25026..27029
                     /locus_tag="IXF54_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1403951.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IXF54_000669"
     CDS             27048..27518
                     /locus_tag="IXF54_000669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1403952.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IXF54_000670"
     CDS             27539..28123
                     /locus_tag="IXF54_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1403953.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IXF54_000671"
     CDS             28138..29124
                     /locus_tag="IXF54_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1403954.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IXF54_000672"
     CDS             29114..29425
                     /locus_tag="IXF54_000672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1403955.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IXF54_000673"
     CDS             29447..31228
                     /locus_tag="IXF54_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1403956.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IXF54_000674"
     CDS             31221..32597
                     /locus_tag="IXF54_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1403957.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IXF54_000675"
     CDS             32604..33239
                     /locus_tag="IXF54_000675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1403958.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IXF54_000676"
     CDS             33269..34624
                     /locus_tag="IXF54_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1403959.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IXF54_000677"
     CDS             complement(34816..36210)
                     /locus_tag="IXF54_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1403960.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IXF54_000678"
     CDS             36302..37273
                     /locus_tag="IXF54_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1403961.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IXF54_000679"
     CDS             37270..38052
                     /locus_tag="IXF54_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1403962.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IXF54_000680"
     CDS             complement(38221..38592)
                     /locus_tag="IXF54_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1403963.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IXF54_000681"
     CDS             38726..39169
                     /locus_tag="IXF54_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1403964.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IXF54_000682"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IXF54_000682"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1403965.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IXF54_000683"
     CDS             complement(40040..41407)
                     /locus_tag="IXF54_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1403966.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IXF54_000684"
     CDS             complement(41391..42221)
                     /locus_tag="IXF54_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1403967.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IXF54_000685"
     CDS             42384..42821
                     /locus_tag="IXF54_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1403968.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IXF54_000686"
     CDS             42897..43517
                     /locus_tag="IXF54_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1403969.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="IXF54_000687"
     CDS             43706..44212
                     /locus_tag="IXF54_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1403970.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="IXF54_000688"
     CDS             44229..45128
                     /locus_tag="IXF54_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1403971.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="IXF54_000689"
     CDS             45215..46099
                     /locus_tag="IXF54_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1403972.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="IXF54_000690"
     tRNA            complement(46291..46362)
                     /locus_tag="IXF54_000690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="IXF54_000691"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="IXF54_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1403973.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47959..48996
                     /locus_tag="IXF54_000692"
     CDS             47959..48996
                     /locus_tag="IXF54_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1403974.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="IXF54_000693"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="IXF54_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1403975.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /locus_tag="IXF54_000694"
     CDS             50148..51338
                     /locus_tag="IXF54_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1403976.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /locus_tag="IXF54_000695"
     CDS             51442..52197
                     /locus_tag="IXF54_000695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1403977.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="IXF54_000696"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="IXF54_000696"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1403978.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17826 a         9357 c        11351 g        15183 t
ORIGIN      
        1 taataaaaaa ggaagaatat tcatgtaaac gattttattg tttttttctc ttttatacaa
       61 aaagggtcat aatttcaagt aagcggatga attcatgagg attttttgca tactgtctct
      121 actatactta ggtcactaag aaaaaggagt gatttattat gaaaaagaac atgattggga
      181 cattattact ttcaacactt cttctaggcg gcttggcaac accagctttt gctgagggtc
      241 aagcgacttc aaaaggagat attaccttta ctgaaccaac aaataccgtg gagcctctta
      301 accctactga tccttcaaaa ccagtagagc cagcagatcc ggaaaatcca gcaactggcc
      361 agacaggtcc tttaacactg gatgtagttc ccgaattgcc atttggtaca cacgagattg
      421 aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa cgatacacct tatctccaag
      481 tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca agggtggaac gtgacagttt
      541 cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg cgctgaatta gattttggaa
      601 catccactgt taaaagtacg tcagataacg aatccactgg accaactagt caaactgtaa
      661 ctgggttgag taaagcaagt gcagcgacac caatatttac agcagcaaaa gatcaaggtc
      721 tgggaacatg gctttctgtt tatgatccag caaatatcac actgaaagtg ccgaaagcag
      781 cagctggaac attcacggct gatttgactt ggaacctcgt ggctggccca gtagcataga
      841 gtatgaaata aattcaacaa actgcggtga aaatactata ggtgaaggat tttttaccgc
      901 agttgttgtt gaaaggatgt ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt
      961 tttttctatt ttaagtctat ttttcatttt tccgtcaatc agccaagcaa aggattcagc
     1021 aggagacttt ggaatcaaac cggtatttcc agagaaccaa atagataaag caatcggtta
     1081 ttttgattta ttagtggcac cggaacaaaa tcagacacta gaagtaatta tcagcaattc
     1141 ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg
     1201 aacgatcgat tatagtcaaa aaaacccaac gttagatgag acattgccgt ttgatgtccg
     1261 tgacgttttg ctcattgcga aaaaggaaat caatgtatca gcacatgcag aaacaactgt
     1321 acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga cgtgtattag cgggtattca
     1381 tgtttcacct aaagaagaag ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa
     1441 tcgtattgct tataatctcg cagtcgtttt acaagaaagt caagagacga tagaaccgga
     1501 tctgaaactt ttatcaggag atttggacga ggtcaacgca aagccaactg ttcagcttcg
     1561 tttccaaaat cctcagccta ggattatcag caatttgata tttacgagca agatctttta
     1621 tgaaaatcag ctttatattg aaaatacgtc taatgcattt ttagttgcac caaattcgaa
     1681 ttttcacttg aatcttgatt tagctggtga taaagcaaaa gccggcgatt accgcgcaga
     1741 aatcatagca aaatcaggcg acagtaatga atggcgattt acacaaaact ttacgatcaa
     1801 aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt gcagtacaag aacaatcttt
     1861 cccatggctt tatgcaggat tggttgctgc agttctagtg ttatggttga ttttgctgtt
     1921 tttgtgggtc cacaagaaaa agaaaaaagg agaaacaaca tgatgaggtg atttcgttga
     1981 aaacatcgat cttattggga gggattattg caagtttgct gttcttcagc tggcaagagc
     2041 cagtccaagc agataccaaa agttcaggta cgattacatt ttatgatcta agtccaattc
     2101 cgcatccaag tggttcagtc aatggcgcat caaaacagca gacacagcta ccgatttata
     2161 aagaatctaa tcaacatggc gtattcccgg agacgaatga acaaaagatc cgttttcttt
     2221 atttgatcat tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg
     2281 aaaaggagga tgaacatgtt gctacaaaat aaattttcat tttttcgctc cttctttttt
     2341 acttcgcttt ttgttttaag tatatttatc ttttcacctt cggcagaagc tgttggagca
     2401 acgcctcctg ccatttctta tgatccatac acgtatgaaa atatcgtacc tccaaatggt
     2461 ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa ctggggctaa ccaattgcct
     2521 aaattgactg ctacttattt gaacgatggt tcaaatgatc cttggaaagg tggcacggtc
     2581 cgttttcatt gggaaagcca tgtgaaacta acaaatgtac ttgatttggt gaatatcact
     2641 gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc aagcagtgat
     2701 gtattaaaag caattgactg gaacaacgcc tacttgtata ttgccggtgc ccccatcaag
     2761 gttactagcg gaagtttatt atcagcagga agccatacct tgcgttttgg gatggggtcg
     2821 tgggatacaa atactctatt gacagatatt attgctatta ttacgcgagg cgaattcaac
     2881 gtggacgata tcccgcttca atttgatgtt acagctaatg tcgcgaagat gacagaaaac
     2941 ggagataaat atgatacgag tccaaacaaa attttgacca agggtaaatt acaaccttct
     3001 atgtctaaaa aggccgtttt ttctgttgat ttttatgatt cgaataatat tatccaagat
     3061 cgtatgttca attcgatttt agctccagct gcaggtagat tttatcctaa attaactggc
     3121 ggaaacttga atccaggagc atatgcattt tctgcaagtg cagagataaa tacgtggaat
     3181 agttacatct caccaagtga ttcaacagga aaatataata catttgctga taccacagaa
     3241 tacgtggatg gaaataacca gaaccttcca ggctctacca atagtcggac attgacgatg
     3301 gggctgactg aaggacgttt tgatcagttg ccatttagtc gctttgatcg cgtagtcaac
     3361 tattttacag gaaaaaacgt tacacaagga gcaatactga ctcatactcc agcaagcaca
     3421 agtggattga atcagacagc caatatcgtg tacagtggga aagatgcgca aggcggattg
     3481 ctttctcctg ttgctttaaa ggtattggag cagtacaagc tagacggaac ggttttacca
     3541 acaaacttaa cagccgatag tcagtgggga aagctccttt cttctgcatc gtaccaagtc
     3601 acggccaatt ggtcagcaac tgaactaaaa aagggttcga tccattacag gctttattcg
     3661 aaaggaaccc aaaaattagc gacaggatgg acagatcaac ttttccaaac gatccaatca
     3721 aataacgggg gtgtaaatac agctacttct acactacctg cattggcttc agggcaatat
     3781 tattttgatt atcgcttggt ggacgatgta cttactaaca tgtatccgtc ttttgcgtac
     3841 aaatggcagt ctgaacaagc tggaatcacc actcttcctc aaataacagt ggctaatttt
     3901 ccatctgtga gtgcagatag tcagctaaaa aaccttagca gacaacctga gacaggagat
     3961 cccctgtctg ctctatcagg agatacgatt caagaaaatg tgacattcac gttaacaaaa
     4021 tctggggatg cgataaccga caaaaaaatc acgatctcac ttcctgaaaa tactacttat
     4081 atcaatggtt ccttaaaatt aaatggtaca actatcgcag ataccggcat tcaacaagga
     4141 gtttctgtcc cagcagatct attgagcaaa ataggagata ccattcatct tacttacaac
     4201 tatcaattaa atactgtaga tacttcttta caatccgtca gcatactgac aaaggcagct
     4261 gtactttcca gcaatattac attagcagat ggtgctaagc ttcctaaccc agttgtccaa
     4321 acatctgcta aaacaattct tgtccccaaa caagaattaa cattagttaa tgtaccagat
     4381 gattttacat ttggtaacga tctccccaaa ccgttgaaga caagttatta tgaatcaaaa
     4441 ggcgattttt cttttgatgt gcgggatact cggcttccaa gcacgtctcc ttggcaactg
     4501 acaggtacat tgacttcttt atttaagaat gatcaaggtc aagaattaag cggaacaaaa
     4561 ttatacttca atcattctgg atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt
     4621 tatgagagtg atggaacagc taaaggagag gttctcgttg actttcccga taccgatggt
     4681 ttactcttag aggtaaatag cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg
     4741 gtcacatggg aactgacagc tgggccaacc agctaagtga ttagaatttg tatggatctg
     4801 tgataactgt cacagatcct ctttttcaag gatgagaagg aaacttctgc tgttaaaatc
     4861 agaaatgctt gaaggagtgg acttggaact tttcagtttg cttgaaaaaa atgaacaacg
     4921 gcaaatcaaa cttttccaac ttttattaga ggaaaaagga gaggtttccg ttaataacgt
     4981 acttacccgc ttatcagttg atcgggctac cttgaaagaa gatctgtttt atttacaagc
     5041 taacttggaa aatatcaaac aattggaaat tgttatccaa gaagggaata ttttattaat
     5101 cagacatgga aatatagcag cagtcgatgt ttattatcat tgttacttgc gctattctac
     5161 aaaatatcag atacttcttt atctgttgga aaatggctca tttgatcggc agaaacttgt
     5221 catggaactt aatatcagtt cggctacttt gtctcgcaga gtgaaggaat taaatgattc
     5281 tttaaatgag tttcatctga aaataaaaag cgggcggctg accggtccag aaagtcaaat
     5341 tcgttatttt tatttccaat tattttggtt tggacgtccc tatcttgaaa acaaaaaaga
     5401 atacagtgat ttatcaggaa acactttttt agatctgctg caaaaagagt ttagttttgc
     5461 tattacagaa gaaggacgat tgaaatttga aatatggctt tccattatga agaagcgaat
     5521 gcgctgtacg aaaaaatcca aaccagatta tagcaatctt cctgttggat tgtttcaaac
     5581 gacacaattt ttattggacc tccatacatt tttgacccgt tacttctttc aatatgcctt
     5641 tccttggaat caaaatgaaa cgttggtttt ttatcttttc atgatcagta actttacatt
     5701 agatacacat aactcgcatg tccaggagtt tttgataagc gaaaaataca aagacgacgt
     5761 agtagctcaa ttgaatcgaa cgtttaaaca atcgatagaa gaatatttct ctttttatgt
     5821 ttttccagaa gagatttcgc aaaagattgc gttgacagtg atgcaaaatc acgctcattt
     5881 tgcctatttt agaggatgga tgaatatttt tggccaagaa ggattaaaac agcggttaac
     5941 tgaagtagaa gataaacaat tcctggtttt atgtgaaaga ttggtcagac aaacatttgc
     6001 tattgttgat tttactgaag tagacgagga aaatgcccgc tttgaattat ttgctcgcta
     6061 tgcatctatt ttacggacgc tgatcaagca gttagaatta cgtctgcaaa ttgcttatga
     6121 tcttccttat gaaaggctaa tggcggatat cgtgaaagaa atcatccagg aacaattgct
     6181 gaatacgatc aaatatgatt tagtcgaata cgagaaagaa aagcagtatg atatcatttt
     6241 gactagccaa ttgaaagagt acccgtcaca gaagtccgct aaagtttttg tattcactag
     6301 caacgaatat aattatgatt tcccctattt gaaccaattt ttaaaagaat gttatttaga
     6361 aaaattgaat ttaaggatga ataaaacatg aaaatcgtga tcattggtgc ttcccatgaa
     6421 ggcatcacag ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt tttactgatc
     6481 gatgaagatc ataaagatgg actaggctat gtttcaaatg gaatcaacct ttatttaaaa
     6541 ggaaagatac gtagtttagc tgaagttgcc cataacatgc ggactcttca agaaagtggg
     6601 gcaaaactga tcaccgaatg gagagtaact gaacttgatc cagacaaaca tcagctcatc
     6661 ctcgcatcca aagaaggaaa aaacgagacc ataacctatg ataaattaat cgtagcgaca
     6721 ggttcctctc ctgtcacctt gtataaacaa attgaagctg aaaatgtcta cacctataaa
     6781 aatctagtgc aatctaagca agtacttgcc gctttaaaag aagcaaaaga agtagtgatc
     6841 ttcggtgctg gttacattgg attggaatta gcagatgctt tgagaaataa aggatacatt
     6901 attcatctag ttgattatat gccgaatgtt ttatcaagat atttcgataa ggatatgatc
     6961 aacagttttc aaaatcagct gcaaactaag caaataaatt tttatccaaa tgaattcttg
     7021 atcgattgga aaaaatcaga agagaaagta gtatccgtac aacttctgtc tcaaacaata
     7081 aaggcagata tggtcatttt ttctgcccaa actcgtccta atacgacatt gttaaaagaa
     7141 aaagtggctt tgtatgagga tgagactgtg atagtcaatg aatatctgca gacaagtgat
     7201 ccggacatct atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat
     7261 ctctttttac ctttagttac tcgagctgta catatggcaa gagcagtagc gctaactttg
     7321 agtggtcagc caaccgctta cgacctcaga caaaagataa cagcagcagt cattatagac
     7381 tactttttag gaactgtagg gctgacagaa gatgaagcgc cattcttgga acaaaatacc
     7441 ggttcgtgtt caggagaatt tgatctattt ccacaatacg acgaagaaaa caaaacagta
     7501 aatgcaaagc tgatctacca tccggataca ttagaaataa ttggcggaca gctgataagc
     7561 caagaattct tattatcaga tttgaacctg cttgcggata tcgtgaaaca caaaactacg
     7621 attccacagc tagcagttga ggattttgga tttctgtctg aatacacctc tcgttttcac
     7681 tacctcaatg agttagcttt taaagtacta gctaagagag caaatagcaa tagagtagat
     7741 aaaagactgt gagaaatatc acggtctttt attgtgtggg gatataataa atattaatca
     7801 atcgagaata aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg
     7861 ccaaaaattt atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt
     7921 aaatgttttt agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag
     7981 gggtgatgtt tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag
     8041 ctcaagataa tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag
     8101 atgcaaattc aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag
     8161 gtgggaaagt ggcgtatctt gcccaaacac ctggagtcgt gactagtaaa gacagtacag
     8221 aagaaggaaa aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca
     8281 atcttgttga aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga
     8341 aagaagaata tggtctagcc ataggagata cagttaagtt atctggtagt gacaagacgt
     8401 ttaaactaac tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa
     8461 ccatcaatgc ttaccaagaa atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga
     8521 ttaacgcaat agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca
     8581 aaatttctaa attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat
     8641 ttatgatcgg atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac
     8701 tgacgatgca aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat
     8761 ttattgcgcg ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg
     8821 gtctattagg aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca
     8881 attggctgtt ttttggcgtc attagcttgt tgatgttagt agtagctgtg ttaggcgcac
     8941 tattttctgt tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag
     9001 gaagaacatg aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca
     9061 agtagttaaa gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat
     9121 catcggtcct tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac
     9181 gcctagcagt ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagcga
     9241 acgcgttaaa ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc
     9301 ttttctgaca gtaaaaaagc agttattatt agtagataag attaaacatg aacatcgtca
     9361 aaaagaagca aacgaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc
     9421 agaagaacta tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga
     9481 tccgtccatt attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga
     9541 agtagtcgat atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac
     9601 tcacgataca cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt
     9661 gaaagaaaaa gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata
     9721 cacagcaaaa aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca
     9781 cttcaacaag agaaatcgca caaaaagttg ggatcaccca gcccaatctc tatcattatt
     9841 tcggtgataa agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa
     9901 aagctttgag agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa
     9961 tggcgcagtt tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg
    10021 aaagcaatct ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc
    10081 gtgaaccttt tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa
    10141 aagaaatcgc agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa
    10201 gacctgtgga agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat
    10261 taggagaagc gtaaagctgt tatataggga gaaaatagtt aggtgaagaa gcaagatatg
    10321 acgaagatcg ttatatcttg cttctttata taggtagttt ttcataaata caaatttgtt
    10381 attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc aggaagtgtt
    10441 ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt tattgggtag
    10501 atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca
    10561 ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt ttgcttactt
    10621 aagctgattt taagttttgg cccttagtct ggatctacga ggtgagagtc aagaatgaaa
    10681 aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt tcaaatagat
    10741 gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag tacggttaca
    10801 aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat cggaaaaaat
    10861 gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat aaacggcgga
    10921 gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac aaatgatggc
    10981 tatactggat atttagccct cgaagatgga accaaagtca caaagaatat acaaacaaaa
    11041 ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg aaatgatact
    11101 ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag tacagccata
    11161 aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca tactacagat
    11221 gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt
    11281 atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat cacgacatat
    11341 tattactatg gacaagagga aacattagca cagacaattg ctgactcctt cactgatctg
    11401 cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga aaatcttcaa
    11461 ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa ggaattagta
    11521 acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac cacttatttt
    11581 cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt ttggccgggc
    11641 ttattatcgc agatgcaagc ttttaaacac cgtttaagga agaggtcgtg agcctgccgt
    11701 tcaactctga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgactattg
    11761 tgacttgtga ccgaggagtt ggcatgagaa caccgtttaa gaaaaaggtt gtgaaatcaa
    11821 cgttttgttc tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt
    11881 gttgagctaa taccgaggat cagatgattg aacaccgttt attcggaaca agggactgtg
    11941 acatgacttt tgtcacagtt ccattatttt tttagtagct tcaaagcttt ttctgtattg
    12001 gcaaaatgaa gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt
    12061 gccgctactt ggtcagattt cgttccagct cctgacggaa gggtgattcc agcttcagct
    12121 gatgctttgt ctaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca
    12181 gctaaatgat attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt
    12241 tctaaacgcg catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct
    12301 tctggttttt ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg
    12361 actttcatcc gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag
    12421 acaagcagtt tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta
    12481 tcttttaact cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt
    12541 ttcacataag cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca
    12601 ctacctaaaa cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc
    12661 ttggcttgtt tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct
    12721 tgaaacatca cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca
    12781 aagctagtat aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga
    12841 ctaagttggt cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta
    12901 tacataaaac tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc
    12961 atttttttaa agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt
    13021 gaaagaacca acttcatggt acactaaata aaactttcat caaataggag gaaatgattc
    13081 gtatggcgca cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta
    13141 tcggacggga aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag
    13201 cagaattgaa acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg
    13261 tcaatgcgct ttcggatcaa ttgaagaaac aagaaagaat attggagctg gaagaagaaa
    13321 cagcagaatt aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca
    13381 ttgaagcaat cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata
    13441 ccattaaaaa tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta
    13501 aacaaaaatc ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga
    13561 agagaaagga acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt
    13621 tttctggagc acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt
    13681 catttattgc tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc
    13741 catacccagc tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcctttc
    13801 acctagatga agctttttat gcaggaactg cttttctgat tattttgcta attggctggc
    13861 tgcttacccg atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac
    13921 aggtagactg gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt
    13981 tgattttgca gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc
    14041 atagtttcgc tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc
    14101 tttgggtaac gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt
    14161 acagagtagt ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata
    14221 gtgaaagata aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag
    14281 tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat
    14341 tggtaccagt gagtgagaaa caaacgatca caaactggct tcaagaaact gaggatggat
    14401 tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa aattgaaaat atccgtcccc
    14461 atatgaaacg gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca
    14521 gagtattgtc tacgacaagc gaattgaatc gtttcattga tgatctttct gacagtgaaa
    14581 tcgaatttgc acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc
    14641 gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt
    14701 taaagagcat aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg
    14761 ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg
    14821 accgctacgt tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg
    14881 atcaaagtgc ttcaggtcag actttattta ttgaaccaaa acaagtggtg gatttgaata
    14941 atcgtttgcg tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac
    15001 tttctgctga acttgttccc catcgtcaag aaatcatcca caatacttat gtgattggta
    15061 aaatggatct gatgaatgca aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg
    15121 gaatcagtga agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg
    15181 aaaaagtagt ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag
    15241 gacctaacac aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg
    15301 ggcaagcagg attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag
    15361 tatttgcgga tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc
    15421 atatgacgaa cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg
    15481 acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag
    15541 atgatcttgg taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag
    15601 tttatggata taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat
    15661 taagtccaac gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct
    15721 ctagccgatt aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg
    15781 agagccaaga tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga
    15841 ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga
    15901 aagaagctta cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa
    15961 aagcaaatga agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc
    16021 gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa
    16081 agacccaatt agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga
    16141 aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc
    16201 aaagagggac gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa
    16261 aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac
    16321 gagttatcca tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc
    16381 gaggaaaacg atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc
    16441 tagctggtta tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg
    16501 gaattactga ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa
    16561 accaaggcgg aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca
    16621 gatggttcgc tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt
    16681 ctttgatatg tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact
    16741 tgtattgatt gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt
    16801 ggatcagttg gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga
    16861 tgaaaatcca gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa
    16921 aaaagatggt caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca
    16981 aatgatcgat caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat
    17041 ccagttccgt cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg
    17101 agtataatta cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa
    17161 ttgaatatga aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc
    17221 ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca
    17281 aagccaaaat attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa
    17341 cggaacggct tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg
    17401 aagcattgct cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt
    17461 taaaggatga taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg
    17521 tgattactcg taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg
    17581 gtgcggcgaa tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac
    17641 ctagtcagac gaaggagcct tgtctttact atcatttggg acaatgcctg tgtccttatt
    17701 atttcgacgt agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa
    17761 acggaggaca cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa
    17821 acatggagtt tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca
    17881 tgactcgcca aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga
    17941 ttgataaagg atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac
    18001 gagatgtttc aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg
    18061 gtcagtttta tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga
    18121 tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag
    18181 aaaagaaaaa attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt
    18241 ttgatctgat tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg
    18301 cgatgaatat cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga
    18361 cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc
    18421 ggaaatataa aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca
    18481 tctatcgcag atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga
    18541 tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa
    18601 tcgatattcc tattgctggc atggtaaaaa acgataaaca taaaacaaat gagttgctat
    18661 tcggtacaga tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc
    18721 agcgaatcca agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta
    18781 aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa
    18841 tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac
    18901 aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat
    18961 gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag
    19021 agcgtttacg ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg
    19081 tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg
    19141 ggaactacac agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc
    19201 taattggcca aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga
    19261 cacaagacca aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg
    19321 tcatcggtta tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac
    19381 tgctttattt tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact
    19441 tttggatgga aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta
    19501 aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca
    19561 ttttggatga gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa
    19621 tattggaatt gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg
    19681 tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa
    19741 aagtacatga gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa
    19801 gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata
    19861 cgttagagat taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc
    19921 agttcggcta cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat
    19981 tgaaagcagg tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa
    20041 aatgttaagt cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc
    20101 atttatgtag caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg
    20161 gctgataatg agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc
    20221 aaagcagttg attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg
    20281 tatttagtag tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca
    20341 ttaggacaaa gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt
    20401 cgagcgagtc aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt
    20461 ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac
    20521 tcgacagtcc aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg
    20581 acgtatgcgc agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa
    20641 gtggatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagaga
//
LOCUS       DACERL010000012        53539 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.012, whole genome shotgun sequence.
ACCESSION   DACERL010000012 DACERL010000000
VERSION     DACERL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53539
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.012"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..878
                     /locus_tag="IXF54_000697"
     CDS             <1..878
                     /locus_tag="IXF54_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1403979.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="IXF54_000698"
     CDS             complement(1119..2735)
                     /locus_tag="IXF54_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1403980.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="IXF54_000699"
     CDS             2853..3071
                     /locus_tag="IXF54_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1403981.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="IXF54_000700"
     CDS             3266..3727
                     /locus_tag="IXF54_000700"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1403982.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="IXF54_000701"
     CDS             complement(3920..4159)
                     /locus_tag="IXF54_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403983.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="IXF54_000702"
     CDS             complement(4183..5706)
                     /locus_tag="IXF54_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1403984.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="IXF54_000703"
     CDS             complement(5724..5846)
                     /locus_tag="IXF54_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1403985.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="IXF54_000704"
     CDS             complement(5876..6859)
                     /locus_tag="IXF54_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1403986.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="IXF54_000705"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="IXF54_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1403987.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="IXF54_000706"
     CDS             complement(7053..7430)
                     /locus_tag="IXF54_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1403988.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="IXF54_000707"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="IXF54_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1403989.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="IXF54_000708"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="IXF54_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1403990.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10220)
                     /locus_tag="IXF54_000709"
     CDS             complement(8448..10220)
                     /locus_tag="IXF54_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403991.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="IXF54_000710"
     CDS             complement(10174..11928)
                     /locus_tag="IXF54_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1403992.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="IXF54_000711"
     CDS             complement(12038..12607)
                     /locus_tag="IXF54_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1403993.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14049)
                     /locus_tag="IXF54_000712"
     CDS             complement(12625..14049)
                     /locus_tag="IXF54_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1403994.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="IXF54_000713"
     CDS             complement(14051..14719)
                     /locus_tag="IXF54_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1403995.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="IXF54_000714"
     CDS             complement(14849..15433)
                     /locus_tag="IXF54_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1403996.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="IXF54_000715"
     CDS             complement(15764..15997)
                     /locus_tag="IXF54_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1403997.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="IXF54_000716"
     CDS             complement(16012..16962)
                     /locus_tag="IXF54_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1403998.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="IXF54_000717"
     CDS             complement(16959..17801)
                     /locus_tag="IXF54_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1403999.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="IXF54_000718"
     CDS             complement(17803..18522)
                     /locus_tag="IXF54_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404000.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19214..19372
                     /locus_tag="IXF54_000719"
     CDS             19214..19372
                     /locus_tag="IXF54_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404001.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            19444..20670
                     /locus_tag="IXF54_000720"
     CDS             19444..20670
                     /locus_tag="IXF54_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1404002.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20758..21150)
                     /gene="rpsI"
                     /locus_tag="IXF54_000721"
     CDS             complement(20758..21150)
                     /gene="rpsI"
                     /locus_tag="IXF54_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1404003.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21166..21609)
                     /gene="rplM"
                     /locus_tag="IXF54_000722"
     CDS             complement(21166..21609)
                     /gene="rplM"
                     /locus_tag="IXF54_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1404004.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22128..22595)
                     /locus_tag="IXF54_000723"
     CDS             complement(22128..22595)
                     /locus_tag="IXF54_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1404005.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(22715..24253)
                     /locus_tag="IXF54_000724"
     CDS             complement(22715..24253)
                     /locus_tag="IXF54_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1404006.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24333..25019
                     /locus_tag="IXF54_000725"
     CDS             24333..25019
                     /locus_tag="IXF54_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1404007.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25220..25747)
                     /gene="msrA"
                     /locus_tag="IXF54_000726"
     CDS             complement(25220..25747)
                     /gene="msrA"
                     /locus_tag="IXF54_000726"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1404008.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(25831..26508)
                     /gene="pgmB"
                     /locus_tag="IXF54_000727"
     CDS             complement(25831..26508)
                     /gene="pgmB"
                     /locus_tag="IXF54_000727"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1404009.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(26505..27635)
                     /locus_tag="IXF54_000728"
     CDS             complement(26505..27635)
                     /locus_tag="IXF54_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1404010.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(27635..29818)
                     /locus_tag="IXF54_000729"
     CDS             complement(27635..29818)
                     /locus_tag="IXF54_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1404011.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(29815..30840)
                     /locus_tag="IXF54_000730"
     CDS             complement(29815..30840)
                     /locus_tag="IXF54_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1404012.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(30885..31712)
                     /locus_tag="IXF54_000731"
     CDS             complement(30885..31712)
                     /locus_tag="IXF54_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1404013.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(31709..32776)
                     /locus_tag="IXF54_000732"
     CDS             complement(31709..32776)
                     /locus_tag="IXF54_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1404014.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(32802..33638)
                     /locus_tag="IXF54_000733"
     CDS             complement(32802..33638)
                     /locus_tag="IXF54_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404015.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(33635..34504)
                     /locus_tag="IXF54_000734"
     CDS             complement(33635..34504)
                     /locus_tag="IXF54_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404016.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(34574..35857)
                     /locus_tag="IXF54_000735"
     CDS             complement(34574..35857)
                     /locus_tag="IXF54_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404017.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(35871..37556)
                     /locus_tag="IXF54_000736"
     CDS             complement(35871..37556)
                     /locus_tag="IXF54_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1404018.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            37831..38856
                     /locus_tag="IXF54_000737"
     CDS             37831..38856
                     /locus_tag="IXF54_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1404019.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(39451..43104)
                     /gene="rpoC"
                     /locus_tag="IXF54_000738"
     CDS             complement(39451..43104)
                     /gene="rpoC"
                     /locus_tag="IXF54_000738"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1404020.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(43183..46800)
                     /gene="rpoB"
                     /locus_tag="IXF54_000739"
     CDS             complement(43183..46800)
                     /gene="rpoB"
                     /locus_tag="IXF54_000739"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1404021.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            47528..48496
                     /locus_tag="IXF54_000740"
     CDS             47528..48496
                     /locus_tag="IXF54_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404022.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            48514..49419
                     /locus_tag="IXF54_000741"
     CDS             48514..49419
                     /locus_tag="IXF54_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404023.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            49416..50222
                     /locus_tag="IXF54_000742"
     CDS             49416..50222
                     /locus_tag="IXF54_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404024.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            50379..51347
                     /locus_tag="IXF54_000743"
     CDS             50379..51347
                     /locus_tag="IXF54_000743"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1404025.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(51664..52188)
                     /locus_tag="IXF54_000744"
     CDS             complement(51664..52188)
                     /locus_tag="IXF54_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1404026.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            52475..>53539
                     /gene="scm"
                     /locus_tag="IXF54_000745"
     CDS             52475..>53539
                     /gene="scm"
                     /locus_tag="IXF54_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1404027.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        16020 a        10918 c         9134 g        17467 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt cttttatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacattt aaaataactt gaaattttct
    19381 tctcgccgca tatactaaaa ttagtgaaat atactgcctt acttttttta aggagtgagg
    19441 attttgccta caaagctttc aaatgggaaa tataaaacta atctgagata tccaaaaagg
    19501 tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa
    19561 ttggcgatta aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt gagagaggag
    19621 aatgcaaact cattagaatt aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa
    19681 tggcttccta gatatgaatt aggtcaaaca attagatcaa acaggcctcc tagtgatata
    19741 accattagta atacaaaaga tatatttcgg ttgcatattt tacctatgtt tggtgaatac
    19801 gcaatgaatt atctaaatat caatactgag ataatatcag atgaattaac caaaaaatcg
    19861 aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca
    19921 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
    19981 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
    20041 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
    20101 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
    20161 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
    20221 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
    20281 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
    20341 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
    20401 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
    20461 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
    20521 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
    20581 tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct
    20641 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
    20701 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
    20761 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
    20821 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
    20881 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
    20941 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
    21001 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
    21061 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
    21121 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
    21181 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
    21241 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
    21301 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
    21361 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
    21421 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
    21481 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
    21541 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
    21601 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
    21661 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
    21721 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
    21781 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
    21841 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    21901 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
    21961 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
    22021 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
    22081 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
    22141 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
    22201 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
    22261 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
    22321 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
    22381 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
    22441 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
    22501 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
    22561 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
    22621 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
    22681 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
    22741 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
    22801 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
    22861 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
    22921 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
    22981 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
    23041 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
    23101 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
    23161 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
    23221 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
    23281 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
    23341 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
    23401 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
    23461 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
    23521 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
    23581 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
    23641 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
    23701 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
    23761 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
    23821 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
    23881 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
    23941 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
    24001 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
    24061 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
    24121 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
    24181 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
    24241 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
    24301 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
    24361 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
    24421 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
    24481 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
    24541 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
    24601 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
    24661 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
    24721 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
    24781 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
    24841 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
    24901 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
    24961 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
    25021 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
    25081 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
    25141 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
    25201 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
    25261 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
    25321 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
    25381 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
    25441 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
    25501 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
    25561 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
    25621 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
    25681 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
    25741 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
    25801 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
    25861 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
    25921 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
    25981 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
    26041 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
    26101 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
    26161 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
    26221 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
    26281 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
    26341 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
    26401 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
    26461 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
    26521 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
    26581 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
    26641 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
    26701 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
    26761 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
    26821 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
    26881 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
    26941 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
    27001 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
    27061 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
    27121 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
    27181 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
    27241 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
    27301 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
    27361 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
    27421 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
    27481 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
    27541 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
    27601 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
    27661 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
    27721 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
    27781 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    27841 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    27901 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    27961 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    28021 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    28081 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    28141 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    28201 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    28261 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    28321 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    28381 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    28441 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    28501 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    28561 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    28621 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    28681 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    28741 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    28801 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    28861 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    28921 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    28981 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    29041 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    29101 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    29161 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    29221 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    29281 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    29341 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    29401 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    29461 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    29521 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    29581 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    29641 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    29701 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    29761 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    29821 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    29881 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    29941 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    30001 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    30061 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    30121 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    30181 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    30241 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    30301 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    30361 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    30421 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    30481 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    30541 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    30601 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    30661 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    30721 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    30781 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    30841 ttatttctcc ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    30901 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    30961 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    31021 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    31081 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    31141 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    31201 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    31261 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    31321 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    31381 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    31441 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    31501 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    31561 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    31621 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    31681 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    31741 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    31801 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    31861 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    31921 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    31981 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    32041 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    32101 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    32161 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    32221 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    32281 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    32341 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    32401 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    32461 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    32521 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    32581 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    32641 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    32701 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    32761 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    32821 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    32881 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    32941 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    33001 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    33061 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    33121 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    33181 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    33241 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    33301 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    33361 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    33421 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    33481 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    33541 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    33601 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    33661 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    33721 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    33781 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    33841 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    33901 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    33961 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    34021 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    34081 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    34141 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    34201 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    34261 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    34321 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    34381 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    34441 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    34501 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    34561 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    34621 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    34681 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    34741 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    34801 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    34861 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    34921 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    34981 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    35041 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    35101 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    35161 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    35221 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    35281 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    35341 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    35401 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    35461 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    35521 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    35581 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    35641 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    35701 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    35761 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    35821 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    35881 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    35941 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    36001 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    36061 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    36121 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    36181 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    36241 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    36301 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    36361 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    36421 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc
    36481 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    36541 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    36601 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    36661 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    36721 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    36781 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    36841 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    36901 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    36961 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    37021 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    37081 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    37141 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    37201 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    37261 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    37321 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    37381 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    37441 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    37501 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    37561 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    37621 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    37681 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    37741 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    37801 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    37861 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    37921 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    37981 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    38041 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    38101 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    38161 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    38221 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    38281 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    38341 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    38401 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    38461 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    38521 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    38581 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    38641 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    38701 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    38761 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    38821 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    38881 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    38941 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    39001 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    39061 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    39121 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    39181 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    39241 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    39301 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    39361 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    39421 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    39481 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    39541 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    39601 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    39661 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    39721 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    39781 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    39841 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    39901 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    39961 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    40021 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    40081 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    40141 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    40201 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    40261 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    40321 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    40381 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    40441 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    40501 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    40561 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    40621 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    40681 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    40741 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    40801 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    40861 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    40921 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    40981 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    41041 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    41101 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    41161 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    41221 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    41281 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    41341 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    41401 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    41461 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    41521 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    41581 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac
    41641 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    41701 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    41761 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    41821 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    41881 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    41941 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    42001 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    42061 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    42121 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    42181 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    42241 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    42301 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    42361 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    42421 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    42481 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    42541 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    42601 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    42661 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    42721 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    42781 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    42841 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    42901 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    42961 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    43021 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    43081 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    43141 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    43201 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    43261 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    43321 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    43381 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    43441 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    43501 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    43561 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    43621 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    43681 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    43741 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    43801 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    43861 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    43921 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    43981 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    44041 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    44101 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    44161 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    44221 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    44281 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    44341 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    44401 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    44461 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    44521 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    44581 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    44641 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    44701 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    44761 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    44821 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    44881 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    44941 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    45001 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    45061 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    45121 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    45181 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    45241 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    45301 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    45361 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    45421 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    45481 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    45541 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    45601 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    45661 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    45721 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    45781 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    45841 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    45901 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    45961 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    46021 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    46081 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    46141 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    46201 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    46261 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    46321 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    46381 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    46441 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    46501 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    46561 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    46621 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    46681 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    46741 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    46801 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    46861 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    46921 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    46981 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    47041 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    47101 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    47161 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    47221 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    47281 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    47341 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    47401 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    47461 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    47521 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    47581 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    47641 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    47701 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    47761 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    47821 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    47881 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    47941 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    48001 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    48061 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    48121 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    48181 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    48241 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    48301 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat
    48361 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    48421 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    48481 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    48541 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    48601 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    48661 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    48721 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    48781 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    48841 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    48901 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    48961 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    49021 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    49081 atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct
    49141 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    49201 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    49261 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    49321 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    49381 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    49441 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    49501 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    49561 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    49621 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    49681 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    49741 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    49801 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    49861 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    49921 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    49981 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    50041 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    50101 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    50161 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    50221 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    50281 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    50341 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    50401 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    50461 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    50521 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    50581 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    50641 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    50701 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    50761 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    50821 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    50881 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    50941 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    51001 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    51061 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    51121 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    51181 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    51241 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    51301 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    51361 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    51421 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    51481 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    51541 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    51601 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    51661 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    51721 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    51781 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    51841 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    51901 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    51961 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    52021 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    52081 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    52141 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    52201 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    52261 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    52321 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    52381 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    52441 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    52501 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    52561 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    52621 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    52681 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    52741 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    52801 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    52861 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    52921 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    52981 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    53041 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    53101 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    53161 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    53221 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    53281 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    53341 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    53401 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    53461 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    53521 tgatagctca gattcatca
//
LOCUS       DACERL010000013        51945 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.013, whole genome shotgun sequence.
ACCESSION   DACERL010000013 DACERL010000000
VERSION     DACERL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51945
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.013"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            156..1001
                     /locus_tag="IXF54_000746"
     CDS             156..1001
                     /locus_tag="IXF54_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1404028.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1282..2502
                     /locus_tag="IXF54_000747"
     CDS             1282..2502
                     /locus_tag="IXF54_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1404029.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKIPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2499..5219
                     /locus_tag="IXF54_000748"
     CDS             2499..5219
                     /locus_tag="IXF54_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1404030.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5203..6147
                     /locus_tag="IXF54_000749"
     CDS             5203..6147
                     /locus_tag="IXF54_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1404031.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6216..7226)
                     /locus_tag="IXF54_000750"
     CDS             complement(6216..7226)
                     /locus_tag="IXF54_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1404032.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLKSSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7406..7750)
                     /locus_tag="IXF54_000751"
     CDS             complement(7406..7750)
                     /locus_tag="IXF54_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1404033.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7743..8177)
                     /locus_tag="IXF54_000752"
     CDS             complement(7743..8177)
                     /locus_tag="IXF54_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1404034.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8315..9052
                     /locus_tag="IXF54_000753"
     CDS             8315..9052
                     /locus_tag="IXF54_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404035.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDTAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9045..10256
                     /locus_tag="IXF54_000754"
     CDS             9045..10256
                     /locus_tag="IXF54_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404036.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10463..11242
                     /locus_tag="IXF54_000755"
     CDS             10463..11242
                     /locus_tag="IXF54_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1404037.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11290..11952
                     /gene="trmB"
                     /locus_tag="IXF54_000756"
     CDS             11290..11952
                     /gene="trmB"
                     /locus_tag="IXF54_000756"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1404038.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12050..13804)
                     /gene="ade"
                     /locus_tag="IXF54_000757"
     CDS             complement(12050..13804)
                     /gene="ade"
                     /locus_tag="IXF54_000757"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1404039.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13936..14865
                     /gene="cpaF"
                     /locus_tag="IXF54_000758"
     CDS             13936..14865
                     /gene="cpaF"
                     /locus_tag="IXF54_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1404040.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14822..15871
                     /locus_tag="IXF54_000759"
     CDS             14822..15871
                     /locus_tag="IXF54_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1404041.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15868..16146
                     /locus_tag="IXF54_000760"
     CDS             15868..16146
                     /locus_tag="IXF54_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1404042.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16121..16591
                     /locus_tag="IXF54_000761"
     CDS             16121..16591
                     /locus_tag="IXF54_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1404043.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16608..16910
                     /locus_tag="IXF54_000762"
     CDS             16608..16910
                     /locus_tag="IXF54_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404044.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16927..17298
                     /locus_tag="IXF54_000763"
     CDS             16927..17298
                     /locus_tag="IXF54_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1404045.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKTDQ"
     gene            17270..17629
                     /locus_tag="IXF54_000764"
     CDS             17270..17629
                     /locus_tag="IXF54_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404046.1"
                     /translation="MPYGQKPISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17801..18808
                     /locus_tag="IXF54_000765"
     CDS             17801..18808
                     /locus_tag="IXF54_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1404047.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18833..20017
                     /locus_tag="IXF54_000766"
     CDS             18833..20017
                     /locus_tag="IXF54_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1404048.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20454..22037)
                     /locus_tag="IXF54_000767"
     CDS             complement(20454..22037)
                     /locus_tag="IXF54_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1404049.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNPYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDESTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22615..22758)
                     /locus_tag="IXF54_000768"
     CDS             complement(22615..22758)
                     /locus_tag="IXF54_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1404050.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22981..23460)
                     /locus_tag="IXF54_000769"
     CDS             complement(22981..23460)
                     /locus_tag="IXF54_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1404051.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23533..23817)
                     /locus_tag="IXF54_000770"
     CDS             complement(23533..23817)
                     /locus_tag="IXF54_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404052.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23895..24089)
                     /gene="ssrS"
                     /locus_tag="IXF54_000771"
     ncRNA           complement(23895..24089)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXF54_000771"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24161..25447)
                     /locus_tag="IXF54_000772"
     CDS             complement(24161..25447)
                     /locus_tag="IXF54_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1404053.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25585..26256
                     /locus_tag="IXF54_000773"
     CDS             25585..26256
                     /locus_tag="IXF54_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1404054.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26222..26710
                     /locus_tag="IXF54_000774"
     CDS             26222..26710
                     /locus_tag="IXF54_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1404055.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26723..27670
                     /gene="prmA"
                     /locus_tag="IXF54_000775"
     CDS             26723..27670
                     /gene="prmA"
                     /locus_tag="IXF54_000775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1404056.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27671..28435
                     /locus_tag="IXF54_000776"
     CDS             27671..28435
                     /locus_tag="IXF54_000776"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1404057.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28668..30881
                     /locus_tag="IXF54_000777"
     CDS             28668..30881
                     /locus_tag="IXF54_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1404058.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30892..31338
                     /locus_tag="IXF54_000778"
     CDS             30892..31338
                     /locus_tag="IXF54_000778"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1404059.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31509..32768
                     /locus_tag="IXF54_000779"
     CDS             31509..32768
                     /locus_tag="IXF54_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1404060.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32857..33804)
                     /locus_tag="IXF54_000780"
     CDS             complement(32857..33804)
                     /locus_tag="IXF54_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1404061.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34198..35490
                     /locus_tag="IXF54_000781"
     CDS             34198..35490
                     /locus_tag="IXF54_000781"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1404062.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35505..37274
                     /gene="aspS"
                     /locus_tag="IXF54_000782"
     CDS             35505..37274
                     /gene="aspS"
                     /locus_tag="IXF54_000782"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1404063.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37383..38258
                     /locus_tag="IXF54_000783"
     CDS             37383..38258
                     /locus_tag="IXF54_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1404064.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38524..40407
                     /locus_tag="IXF54_000784"
     CDS             38524..40407
                     /locus_tag="IXF54_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1404065.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40482..40766)
                     /locus_tag="IXF54_000785"
     CDS             complement(40482..40766)
                     /locus_tag="IXF54_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1404066.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40756..41490)
                     /locus_tag="IXF54_000786"
     CDS             complement(40756..41490)
                     /locus_tag="IXF54_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1404067.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41619..42242
                     /locus_tag="IXF54_000787"
     CDS             41619..42242
                     /locus_tag="IXF54_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1404068.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42434..43555
                     /locus_tag="IXF54_000788"
     CDS             42434..43555
                     /locus_tag="IXF54_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1404069.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43562..46690
                     /locus_tag="IXF54_000789"
     CDS             43562..46690
                     /locus_tag="IXF54_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1404070.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46709..49903
                     /locus_tag="IXF54_000790"
     CDS             46709..49903
                     /locus_tag="IXF54_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1404071.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50049..50756)
                     /locus_tag="IXF54_000791"
     CDS             complement(50049..50756)
                     /locus_tag="IXF54_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1404072.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51521..51919
                     /gene="spxA"
                     /locus_tag="IXF54_000792"
     CDS             51521..51919
                     /gene="spxA"
                     /locus_tag="IXF54_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1404073.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17050 a         9046 c        10757 g        15092 t
ORIGIN      
        1 ttataaatca aaaatacaga gaatagtata taaatgtcct ttcctattct taaaatgatg
       61 tttgtactta cttaaattag gtataattag tatatgtttt gtgagaaatt tagacttaca
      121 ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa ctaattggta
      181 tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa aatgcaagaa
      241 caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga gaagtagaag
      301 acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg ttaaatggtt
      361 ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa
      421 aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat ctcggaagtt
      481 atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta gggagtgaat
      541 tttctatcaa agagctaaac tatgagcttg agtatgaaaa atctatttgt tcaatccctt
      601 ataataatat tgaggaaatt atagaaaagg atgctatcag catttacaaa ttttttattt
      661 ttattcctaa tcgtcaattg aaagctaata tatttaacca tttgaaaaag atgctaaatg
      721 tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat gtatctaaag
      781 gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg agaatcgcga
      841 tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt tttgcaatgg
      901 gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca aatgataaag
      961 acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa ggtagtatag
     1021 aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat agtacttctt
     1081 cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa atcatgtcac
     1141 aatctttttt tcgaataaac ttattttttg aagtcggcca tttatcgtaa tttctttgca
     1201 taatacgaac aaattttcgg ttattgatga aaaaaaacat tgaaaatggt atgataaaag
     1261 aaaaaaagga ggaaattttt aatggtgaaa tttatccatg cggcagattt acatatggat
     1321 cggtcatttg aaggactgac aactcttgat aaaactgtac aggaaaaact attgaagacg
     1381 aatctgaccg ttttatcaaa tatcattgaa caggcaataa taaataacgt tgattttgtt
     1441 ttacttgcag gagataattt tcatcaaaat cgtccttcat taaaaataca gaaacatttt
     1501 tcagaacaga tgaaacggtt agaaaagaac catatacctg tatttataat attcggaaat
     1561 catgatttct atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt ccatttattt
     1621 acatccgaaa cggtagagac aaaaaaaata acgataaaaa gtggagaaac tgtctctctc
     1681 agtggattta gttatcgtca accgtggatt caaaaagaca aagtgatgga atttccttct
     1741 agagaactca cagattacca tattgggttg taccacgggg aacctggcgt ctctcaaaaa
     1801 ggaaactatg ctcctttcca accctcaaag atgcaagaaa agggatacga ttattgggct
     1861 ttagggcata tccatgtgcc aacggtctta aatgaaaagc aaacaattgt ttatccaggt
     1921 gcgcctcaag gtcatactaa aaaggaacaa gcttctacat ctattctact agttgaatta
     1981 agtaaaggaa gttgccaaat acatccgatt aaagtagcag aggtctattg gaagacaaag
     2041 gaaatatcat tgaggaaagc aagaacgaca aaagaggtca ttgttcacat tcgtcagcag
     2101 ctttcaaaag tagaggatgg atttagttta attgagataa aattaaaaga ttatgaccat
     2161 ctcaatacag atgttttgga acggatacaa tcaggagagc tattagacta tttgttagag
     2221 gaattctcag ataggataaa tgatttcttt atttggcgta tttctctcat agagaatact
     2281 ttttatacga agatcccact tgctgcttct gctaaattga tggaacagtt atttcaatac
     2341 tatcagacac ctcaagattt ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg
     2401 gctagaatat tgagcgaact gccggaatat caagaagaaa cacttgaaaa agccaagcag
     2461 ctgcttaatc aagatttttt atttgaggag gatcaggaat gaagatttta aggatcgaga
     2521 ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc aatcagctga
     2581 tctatggggg aaacgaagca ggaaaatcaa cgatttatca atttattcaa gcaatattat
     2641 ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac ggctcagcct
     2701 atggcggcaa aatatggctt aaacatcctg tatatggtga atttgctgtc gagcgctaca
     2761 agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga agtgatgagt
     2821 tactagaaaa actgatcgca cctttgacga aagagttatt ccagcaggta tttactttcc
     2881 agcaagagca gttgatggaa ctagatcatt tgagagaaaa agagctacat gatgcgctga
     2941 tttctttagg catcacgggc agttctcaag tattccaaaa gcggcaagaa tattatcaga
     3001 aagcacaaca attattcaag aaaaaaggcc aaaaactact aatcaatcaa aaattaaatg
     3061 aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga acaggaagct tattttggca
     3121 agttgatcca acaagaacag gaatacgagt cgcaacagca gcagttattg gcacaattaa
     3181 aagaggcgaa caaacaattt tcattagcca gacagcaaga acttaatttt tctttatatg
     3241 aagaatggca atctctcaaa aaaaacagca ctacaggtct tcctgatgag caacttcttt
     3301 tagatctgga gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg gaagaattag
     3361 aagcaacact ggaaaagcat agcgggatgg atcagcagtc agccagatat tatttttatt
     3421 tagagcagga gcaggaacta aaagatctcc agcaacttca atttacagcg gaacaattaa
     3481 acgaagaatg gcaaagatta caaattgaga ctagagaaac aaattcagaa ttgaagattt
     3541 tggaacaaag gtggcattgg cagaaaaatc cgttaccgga agaattttta tatgaagaag
     3601 aatggaagca tcttctggct gaagaaagaa ggattgctga tctagaaagc cagatttctg
     3661 tgcgtctcca aatagctaaa gaacagcaag agatgattga agcagaagtg aatgacttgg
     3721 aagagaccta tccagcatta ttacaaaatc agtcagagat agcaccagtc tcttcatcta
     3781 aaccgtatat gctgatggct ggcggagcaa taatcattgc aggtatattt gtaccaatgc
     3841 ccttgaaagt gatcctttta ctaattggtg caggcatgat tggctatttc ttctacaaaa
     3901 ggcaaccaaa aattaaaact aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa
     3961 aactaggaca acttgatcta gccttagctc aattagctga agcagaagaa aaacaaaaga
     4021 gaactggaga agaaaaacag attcttcagc aagaagtata tcgttttgcg gatcatcggc
     4081 acttaggaaa gatggatacg ctcaaactca tgaaagaata tggacaggaa gtcatagatt
     4141 atcagaaatt atatggcgta tttgagcgga aacataaaag agaacaggaa atacaagaaa
     4201 agctacgaca aatcgaccaa aaatttgagt ttgttaaaga atggttaccg atacaagata
     4261 agacaatttc agaaaaaatg gtattgcttt cccgttttat acaagagatg gaacagatga
     4321 aatttgccag aagctatcag caaaatactt tattgaagca agaaatcaat caattgaaaa
     4381 agaaacaaaa agtatggata caagaataca gcgaacaatt gactcaagca ggcttatctt
     4441 atccttcgga agtacctgtc tttttacaaa aagccaaaca agccgaacag cagatgaaaa
     4501 gatacaatga attatcggaa atgcttactc cactatttcc tgaagagctg acaaaagaga
     4561 aattatctgt aagactgcgc caactgaaag aaaagcaatc ccaactccag caagaagaaa
     4621 aagaagcaag tgaaaatcgg caacgactcc aacttcaagt agaagagttg caaagagatg
     4681 gcacattgga cgaactttat caacaagcta gccggcaaaa agcaaaatta caggaattac
     4741 tggaggaatg gggagcactt gaaatcgccg ggacattctt aggtgatctt tcaacagaga
     4801 tgagtgaaag acaattaccc cagttgttaa gagcagcagg tgattattta ggtattttaa
     4861 caaaaggaaa ttatcagaag atattactga aaaatgatac actgacttta acagacggat
     4921 atacagaatt tcccatctat gagttatcta caggtacgaa agatcagctg attatggcga
     4981 tgagatttgc ttacctctca ttagaagcta accgttcgat atgcccgatc atcattgatg
     5041 atggctggct tcattatgat catcagcgaa agtatcaatt agctgaactt ttagctaaat
     5101 ttggagaaaa gtatcaaata atttgttttt cttctgataa agaaatggta agctattacc
     5161 atgagatgaa ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa aattcgtgat
     5221 ttagtaatcg atgagcagtt tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc
     5281 gctaaaaacg gaaaaaaatt tattgctttt actttccaag atacttcagg tactatcgat
     5341 ggtaaatact gggatgcttc ggaggaggaa atcaataaat ttacagccgg cacagtcgtt
     5401 tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg
     5461 cgcttaacaa gggtagatga gccaggagag cctagtttat acatggaaag agcccctttg
     5521 aagaaagaag aaatggaaga agaaatcaat caattgttgt ttgagatcac caatgcgcat
     5581 tggaatcgta ttgttcgcta tctacttaat aaatatcaaa aacaattttt tgagtttccg
     5641 gcagcaaaaa gaaaccatca tgcatttgcc agtggattag cttatcatac tttgacaatg
     5701 cttcacttag ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc attgttgtat
     5761 gctggtatca ttttgcatga tttaggaaag gtgattgaac tttccggtcc tatgtcgaca
     5821 gaatatacgc ttgcaggaaa tttgatcggt catctggtat tgattgacga agaaataact
     5881 aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag atgttctcgt attacgtcat
     5941 atggttttag cacaccatgg cttattagaa tatggctcac ctgtacgtcc aagaataatg
     6001 gaagcagaaa tattacatca aatcgacaat atcgatgctt cgatgcagat gatgttgact
     6061 tcgattcgtc aaacagaacc aggaaaatac accgatcgga ttttcggtat ggacaaccga
     6121 agtttctatg taccaaaaga tttatagcaa aaatttattt ttttcattca aaacaaaaaa
     6181 agcgagacat aagtcttcgc tttttttgtt ttcttttatt ttgatgaatc agttgtttct
     6241 tcagttgaag attcttttgt tgaatcagtt gattttgtgt cagaactctt tgttgaagct
     6301 gtagtttcac ttgagtcttt cgtacttgat gaagttgttg tgaatgcact caacacattt
     6361 tcaaaggcat catctttgat ttttacattt gcatctttta attcttcacc gattacttta
     6421 gttgtgaatg cattgtcact tagtttagtt tcagtagcga tatcttttag ttgatctttg
     6481 tatttatcca tgtcattgcc tttattttgg tttttcacca ttttgacaac gtagtattct
     6541 gtcgcataag aagtaggatt tgttgtagta ataacatcgg agatctcacc gtctttcaat
     6601 ttgaaagctg cttcttttac ttctgcagga atagttgttg tcgttgagtc gaatttaact
     6661 ttaccgccat cttcttttgt ctctgtatca gttgatttgt ctttggctaa tttagagaaa
     6721 tcatctccat catccgctga ttttttcaca tcttttgctt catcttcact tgataatttg
     6781 atgatttgtg cttctacttc tggatggaaa gatttccaag cagttttcaa atcgtcatct
     6841 gtaatatcta cgtgagcttt caagccagct tcaaaagcta aattgttacg aatatattct
     6901 ttataagtat ctttcgtata tccagcagct tctaattgtg attcaaaagt atcacctaat
     6961 gatttcgctt gtttgtcgta ttctgcatcc acttgcttat cggttacttt atcgccatat
     7021 ttgttattga atactttata aatgatcatc cgttgtacta aggattggtt cgatgattta
     7081 agttttgctt catcatagaa atcagataca gtgatcgttc cgcctttcat cgttgcgata
     7141 tctttattag tatcgcctga acaagctgct agagttaaaa cagccaacgc actagtagct
     7201 gccaaaataa ttgatttttt tttcattaaa atttgcactc ctgtaattag ttattatttt
     7261 tatatattaa ctataccata tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac
     7321 aaaattcaaa atttcatcac attcctagga aatacgcaaa cttttctcac ttatatagtt
     7381 aaatctgttg agaaaataat cattattata tttgttcagg atactctggc agatcagctt
     7441 ttagtttttc tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat
     7501 cttcgatatc ttttttgatt gcttcttgga aagcaggaat cacagattca gctgtttctt
     7561 ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt atttaaatca tttgtcagag
     7621 ttgttgcttc ttccaattct tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg
     7681 ccaataacag acctccgaca gtgccagcag cagctccaaa taacagtcct ttgaaaaagt
     7741 ttttaatcat cgcaggacac ctgctttacg attgtttctt gaatagcttt tagttcttcc
     7801 gatccataac tatcttgatg attgacaaag tgaatagaaa aatcatcctc tttgctgtag
     7861 cgagggacta aatggatatg tgaatgaaaa acagattgat aggctagttc acggttgtta
     7921 ttcaagatat taagaccttt catttctgga aaagcttttt ccaatgcttg agcaatctta
     7981 ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat atctgctaca
     8041 tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca
     8101 tagaccttgt cgtcttcata tattttatag cttgggattt cttgattgat gattttgcaa
     8161 aaaatacagt tttccatata aaacatcctt tctataaaga ataaatactt cagcttaact
     8221 ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt
     8281 ataataaata aaaataaatg gcggaggaaa aagaatgagt ttaattatcg atcatgtcac
     8341 aggggggtac ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac agcctggaga
     8401 gatagtaggg ttgattggac tgaacggagc agggaaaagt acgaccatca agcacattat
     8461 cggtctgctg gaagcttcaa aaggaacgat agaaatcaat catctatcct taaagaaaaa
     8521 tacggaagaa tatcgcaaac aaattgggta tattccagag actccttctt tatatgaaga
     8581 attgacattg agagaacata tcgaagtcac tgcgatggcc tacgatatac ctatggatac
     8641 agcaatgaaa agagcagaaa agttgttgga gacttttcgg ttatctaatc gtcttgactg
     8701 gttcccagct aatttttcaa agggaatgaa acagaaagtg atgatcttat gcgccttttt
     8761 gatcgagcct tctttatata tcatcgacga accattttta ggcttggatc cactagctat
     8821 caatgcattg cttgaattga tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc
     8881 tacgcatatt ttagctactg cagaaaaata ttgtgatcgt tttgtggtct tacataacgg
     8941 agagatacga gcagacggtt ctcttgagat gttgagaaaa gaatttcagc tgccggattc
     9001 ttctttggac gaaatctata ttgctttaac aaaggagcag gcacatgagt agtttttttc
     9061 aaaaacgttt agcaagacat caaaagaaaa tgatgaaata tatgcgttat gtattgaacg
     9121 atcattttgt tttagtttgt cttttcttga ttggcggtgt gggattatat tattctaatt
     9181 ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct cataatctct gtattttgga
     9241 tgctctgttt attcgtgggt aagatggcta cttttgcaga ggcagcagat attgtttttt
     9301 tacttcctaa agaaaaagaa atgcaaacct atttggaacg agcttttcgc tattcttgca
     9361 tttttccgtt gatctttttg atattggcat gcggattggc catgccatta gtggtggtta
     9421 gtacggagca atccttttcg atgtttttcg tctatctgat catattatgg agtttgaaat
     9481 tcagtcatct gcaaatcaag agagcagaat tgtttcgact agatcaacaa gttattcgtt
     9541 attggaaatt aggctggttt ttttcttctc tgatcttttt atttctatca ttttattggc
     9601 taatatgggc aggaatagca ggtgcattag tacaggcaag tctatattac tggtttttgt
     9661 ggaagcaaca agaaaaagga ttggattggg agaaaatggt gcatgtagaa gaacataggt
     9721 tgcatcagat ctatcaattt atcaatttat tcacagatgt tcctgaaatt acggcaaaga
     9781 tcaaaagaag acgctatttg gatcctatat tgactcatat taaaagagaa tcaaaaaaca
     9841 cttatcttta cttgtttgca agaagattgg caagaggttc tgattttagt ggtctttatt
     9901 tccgattagt aattataggt gggttattga ttgccggagt agtcgacttt tattttagtc
     9961 tgattatcgg tttcttattt ttatatttga tcggtttcca actgttacct ttagcgaacc
    10021 agttccgcta tatggttctc gttcagcttt atccaatcca taagaatcag aagaagcagg
    10081 cgattcagta tctactcaca tgggtattaa ttatcgcgtc ggttattttt ggtttgattg
    10141 ctgcagtcgt attagatggg cagcagcgat gggttccgct agcggcttat ttgattgtta
    10201 ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac
    10261 aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt
    10321 cgttctattt tttttgagaa aagctggttt tttgaatggc aattaaatga aaaagaacgt
    10381 atatgtgctt gacgtgtagt gttcaggcga ctagaataat aagtactgca aaacaaagag
    10441 taagaggacg ggagaatgca tgatggagtt tcagttagac caagagtggc ggttacgtcc
    10501 catcaaaggc gacacaggaa aaacatacat aggcatgcgt gatgaggaca aagtctttat
    10561 taaacgaaac acgactccga tgttggctgc tctttcaagg gaaggaatta caccaaaact
    10621 tgtttggaca aaacgaacgg ggaatggcga tactttgaca gcacaagagt ggctggaagg
    10681 tcgcttgctg actcctaatg agattggtca aagaaacgac gtgattgatg ttttgtacca
    10741 tttacatcat tcaaattctc ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac
    10801 gccggagaag atgctgcaaa aatatgaaaa agaattaccg gaagaactcc agaaaaatca
    10861 atttctgcaa attgtccatc gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa
    10921 cttatcagta gtacacggag atgtaaacca tcgtaattgg ttagtatgta aaaattattt
    10981 atatctagtc gattgggatt caatcatgtt tgcagaccct gcggtggata ttgcaacgat
    11041 tttaggaaat tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt atggcatacg
    11101 cccgactgac gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt tgctccaaga
    11161 aatcaccaga tattgcaaac gtgaagatca tcgacggatg aacgaagaaa ttttacaatt
    11221 aaaacgaatt ttcagtggct gaggtttggc cagacctttt taattagagg aaaggaatgg
    11281 agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg
    11341 aattttgtaa ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac
    11401 gatcatccaa tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca
    11461 aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt
    11521 gcactagaca agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga
    11581 tcagcgttga cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc
    11641 gatccatggc caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct
    11701 gtagatgaac agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga
    11761 ttgtttgagt attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg
    11821 ttagatttac atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt
    11881 tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag
    11941 aaaagtctgt gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatcc
    12001 cagaaaatga acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat
    12061 ttatttcatc ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc
    12121 ctttgtcggt gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa
    12181 aaggatcaaa ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact
    12241 gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac
    12301 aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat
    12361 tagcagcata cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat
    12421 gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc
    12481 caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga
    12541 ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta
    12601 gactattcgg gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg
    12661 aatgtggatt taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat
    12721 gatcagcaaa taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga
    12781 aaacttcatg aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc
    12841 caggggcaat cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca
    12901 tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga
    12961 ttgatccttc ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc
    13021 ttgaattttc aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt
    13081 ctcgaaccat taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag
    13141 cttcatgatc cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac
    13201 cagctgcatg cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga
    13261 tgtctgtttc attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt
    13321 cgggtttttg taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa
    13381 aaggggttac aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg
    13441 catcttctat cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat
    13501 ctgtcactac agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata
    13561 aaagtgagct ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat
    13621 tagcattatg tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga
    13681 ttgctacatc tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga
    13741 tcactaggtc agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt
    13801 ccaatggagt gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca
    13861 atattttttt gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt
    13921 gtggagggga gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt
    13981 gccaaacatg cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt
    14041 cgctatggaa aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg
    14101 atctcaagat ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta
    14161 ggtgcattta cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga
    14221 gattaccttc aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc
    14281 catcgttgct ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga
    14341 ttataccttt tcagcggacc agtcggatca ggcaagactt ctctaatgta tcggttggct
    14401 ttggaagaaa aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt
    14461 tttctgcagc tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc
    14521 gcattacggc atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca
    14581 catgctgcgg taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc
    14641 aatcttgaag gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct
    14701 gaatgtttag ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct
    14761 gttttatgga gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt
    14821 tatgaagaag ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag
    14881 ccagcaaaac cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca
    14941 agaatccctt tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat
    15001 ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg
    15061 agcagagcaa ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt
    15121 gttggaaatt gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct
    15181 gcttgcatat ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt
    15241 gttcgttttg cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca
    15301 gctgatacaa gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc
    15361 tttactagta aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag
    15421 cagacaatta ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga
    15481 gattggaaag ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga
    15541 aacgaaacaa gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc
    15601 aggtatgtca ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa
    15661 aattgtttta cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga
    15721 atggacaaga aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat
    15781 catttttttg ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt
    15841 ttacggaagc atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt
    15901 tttacattga tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc
    15961 gtccctaatt tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc
    16021 cagttggtag acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact
    16081 gtggaggaat tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg
    16141 ccttgaaggt aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt
    16201 attacttaca acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt
    16261 ggcagtctat caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc
    16321 tattgtgaat cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt
    16381 tcatgttccg aatccagata aaataaattg ggaagtatta gatattcctg atgagattac
    16441 cttgaaacgc catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa
    16501 agcttatcaa ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg
    16561 gagtggtcgc tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta
    16621 cagcgcttat gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc
    16681 ttcaggcaga aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata
    16741 tcaagacgca gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg
    16801 atatccaaat tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag
    16861 aagcgatcag tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt
    16921 ggactgatga tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc
    16981 caagtaagtg aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa
    17041 ttagaagaag aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac
    17101 acgatcgagg ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga
    17161 acttccggcg gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa
    17221 gaacacacaa tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg
    17281 acaaaaaccg atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt
    17341 atttctttta tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga
    17401 tcttaatata cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc
    17461 ggaacagtct acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt
    17521 aacatatcag tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt
    17581 tcggttcaca gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg
    17641 tgaaaacgcc ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca
    17701 caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg
    17761 tgaaaaaatt gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga
    17821 acaagggttt aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc
    17881 ctttttagaa gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt
    17941 ggtggatgga gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt
    18001 gaagaaactt tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa
    18061 gggcagccag acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt
    18121 tttatttgtt tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct
    18181 ggatattgcg gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc
    18241 ggggtaccaa acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc
    18301 ttctacaagt gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga
    18361 attattaata gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa
    18421 tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca
    18481 tttattgcta gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat
    18541 gccgagtgga tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga
    18601 aggttatctt caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca
    18661 aaaaagtatt ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt
    18721 gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt
    18781 taaaaactgg aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa
    18841 aacaattgca atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga
    18901 agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac
    18961 catcaagtac ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt
    19021 agcagtaaaa atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga
    19081 aatcaccgga gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt
    19141 gatcgatgag aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa
    19201 tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt
    19261 ggctgtattc gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt
    19321 accggtgtct tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca
    19381 tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat
    19441 catcacttgc cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat
    19501 ggatacttct atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga
    19561 tatcgatcca gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat
    19621 gatcaatgtt ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat
    19681 gcgtgatctt gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt
    19741 ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat
    19801 tgtctttact gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg
    19861 tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt
    19921 agatatctct acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact
    19981 aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa
    20041 agatgaaacg catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa
    20101 aatggtaatt taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct
    20161 tggaaaagtg gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc
    20221 caatatttta ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat
    20281 ttttagatca gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac
    20341 cgatccgaaa cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct
    20401 gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca
    20461 atcagttggc cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg
    20521 aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct
    20581 tgcagcattt cttcaatttc ttctcgagca tagctgtcga ggtgattggt tgattcatcc
    20641 aaaatcagga cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca
    20701 ccaccggata aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct
    20761 aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg
    20821 ctatcgggat cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct
    20881 aatttgatgg tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat
    20941 tttcctgcac cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg
    21001 ctgtctttga atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact
    21061 tctttgccag attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc
    21121 ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag
    21181 gactcattgt ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc
    21241 tggacttctt tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc
    21301 aagactcgaa aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc
    21361 catgtttttg tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat
    21421 ttttttgtat tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg
    21481 gtcggttcat cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact
    21541 ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt cctgacggtg tggaatccct
    21601 aagccttgca agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag
    21661 cgatgctgca gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc
    21721 atcaatgctt cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg
    21781 agcaagtagg ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc
    21841 tttaagcctt ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt
    21901 tgtaaaaagg ttgatttgcc agttccgttg ataccgacta aggcaatttt ttcaccagct
    21961 tcaatttgac aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa
    22021 attgcttgta tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatcca
    22081 tggcctgtgt tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat
    22141 tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa
    22201 ttgcatggct aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca
    22261 tgcgcatcat agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg
    22321 tcagaaagtt caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag
    22381 agagcttttt ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc
    22441 taaaccagcg ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt
    22501 ccttttgcca tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc
    22561 aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt
    22621 cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa
    22681 ttttagtttg gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga
    22741 ttcatcggaa acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa
    22801 accacaccag ggcgactaat cgccctggtg ttcacttttt tttattttac ttgaactcat
    22861 tgactgtttt ccctttattt taaacattat aaagaccgat gagtgcttgc cgcgtctttt
    22921 gtttttatca cggttttttg tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg
    22981 ttaagctttg gtgtggtctt tagctggcaa ttggttttct aaatcagttc ggacaacaag
    23041 cacatcgcaa gctgcgtttc taataacata ttcagaaaca gaaccaataa ataaacgttc
    23101 tacagcattc aatcctgtgg ctcctaacat aatcaaatct acttgattat cttctgggat
    23161 ttctctggca ataatttgtt ttggtgagcc atattctacg acagaggtta cattattcaa
    23221 gccagctttc ttcgcattgc tttcataatc tgccaatgtt tgctttgcca tttcagtagc
    23281 ttgttctgca agcattccat caaaggatga aacggtttgg aaagaacgtg tgtcaatgac
    23341 atgtgctaat agaagttctc cgttatttcg gatagctaca ttgaccgcct ttttaaaagc
    23401 taattcagct tcatcagaac catctacggc aaccatgatt tttttgtatt gttgtaacat
    23461 aagcattcac tcctcaagtc aatcgtattg tactctttta ttgtagaaca ctttagggaa
    23521 aaagagaatt atttactgct ttggttcagc aatttttttt gcaaatcgga tactgaaggt
    23581 ggcagaaaca ataattacga tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa
    23641 agctgttaaa atacccatta cgcctaaaac agcatatgca gctccgtaaa attgtaggaa
    23701 tccttgattt ttatcatttt tgttgatcaa tacaaaaaag atatcctgtt tattaaataa
    23761 ataatagcta acgaataata atactaaagt ggcgataatc attaaaatcc taatcatgaa
    23821 caaatcctcc aaaataagca acaaatagaa aaagcaattt gccaagagca aattgcttta
    23881 ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc
    23941 gaattcaagc aggtgggtcc catggtcgta gcttcgaact accaaatgat aaggcatgtt
    24001 accagcagtg acacaagacg ggtcccaaga tatcaataaa aatgttcggt caacacacaa
    24061 agaggacaat tcgaacatca cagatttccc acctctatag tagcatatga atttaaaaag
    24121 agcaatgaga gagctcgcag aaacagaacc gttttcttgt ctagtttttt ggaggatgct
    24181 ttttccactc ttttatacgg taatactgtt gacccagagc ctgttcatat ttccctgtat
    24241 cttttggctg ataatactga gcattcttca gtttatctgg taaatattgc tgattgaccc
    24301 aagcctgatc aaaatgatga ggatactgat aacccacgcc acggttgagt gatttcgcac
    24361 ctttgtaatg gctatctctc aaatgatcag gaacatctcc agctttgccc tctcgaatat
    24421 cagcaagtgc tgcatctaat gccatgtagg cagaattgga ttttggcgac aagcaaagat
    24481 cgacgacgac gtctgctagt gggatacgtg cctcaggcaa tcccaatttt tcagcagcta
    24541 atacagcgtt cactgtacga gccgcagcag caggattacc taaacctata tcttcataac
    24601 cgataaccat cagacgccgg caaatacttg ccagatcacc tgcctcaact aaacgggcga
    24661 gataatgaag tgcagcatcc acatcacttc cacgaatcga tttttgaaaa gccgaaataa
    24721 cgtcataatg agcatctcca tttttgtcat gggtaagagc ttttctttgg acacattctt
    24781 caatgatcga cagtgtaaga tgaatccttc cttctttatc tgaaggagtc gatagtgtag
    24841 ccagttccag cccattcaat gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa
    24901 gaagagcatc ttcgtctaac tggattgctt gttgtccaag gccgcgttct ttatccctta
    24961 aagcatgttc gactgccaac tggatgtctt gttctgttaa tggtttgact tcaaagatct
    25021 gtgtacggct gcggatcgct ggattgatgg taatataagg attctcagta gtcgctccga
    25081 tcaagatgat tcggccactt tctaaatgag gaagcagaaa atcctgtttt gttttatcta
    25141 atcggtgtac ttcatccaat aagaggatca ctgtaccact catttttgct tcttccgcta
    25201 caacttgtag atcctttttt gaatctgtag cggcgtttag catgcgaaat gcataattag
    25261 tagagccggc gatcgcactt gcgatactcg tttttccagt accaggaggt ccatatagaa
    25321 tcatggaaga gagcatgcga gcatcgacca tgcggcgtat gatttttcct tccccgacta
    25381 gatgctgctg accaacgact tcatcgatcg ttcgaggtct catgcgataa gccaatggct
    25441 gttgcatagg attttcctcc ttcttgtttt tacgtccctt aattatatca tatttagcaa
    25501 gatgcctaga aagaacagcc ttctgttgtt ttgttttcat agcttatcca actaatattg
    25561 tgtatgataa agtaggtgag aaaaatgacg gataaacaaa agatagatga atttttttgg
    25621 aataaaacga cgccagaagt ggcacgggat ttattgggga tgtatttgga gcatgacaca
    25681 ccagaaggaa gattagctgg atatattgta gacgcggaag catacctagg tccagaagac
    25741 gaggccgccc atagctatgg tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa
    25801 ccaggtacga tctaccttta cacgatgcat actcatcgga ttttgaacat cattactcag
    25861 ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg aacccgccgc aatgattgat
    25921 caaatgagta aaaatcgtgg cggaaaaact ggtccagata tcagcaacgg tccgggaaaa
    25981 ttagtggaag cattagcaat tcctcaagaa ctttatggtc agtctatagc agatagttcg
    26041 ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc
    26101 ggtatcccaa acaaaggtgt atggacagaa aagccgttgc gttttgttgt gtcgggaaat
    26161 ccttatatct ctttacaaag aaaaaaccaa atcgagaaag attggggttg gaggaaagaa
    26221 aatgaaaaag aaggatctat taacatattt agatgaaata attgaaaaac aagtgacaga
    26281 ctatgatgta gctttagatt gggatacaaa aaatcatacg atcgagatcg ttgttcgctt
    26341 gtttgcagaa aataaagcac aatttgagat tgatgatgca gaagggatcg tttcgcaaga
    26401 accaattatc gaatttgaag acggcgtact tctttttaat cctcagaaat ctgtttttga
    26461 tgaacgcaat tatctagcag taatccctta tgaaggaaaa aaagggttgg caaaatcagt
    26521 agctgattct ttagtggaat atctaaatga agtattagct caagggcaaa gcgatttgct
    26581 tgattttttg gatgaagacg atgacgaagc tgtattcgag cttcactggg ataatcaaca
    26641 actagcagaa ttagtagaag taaaacagca aaatgaagca gatacttatt taccgtaccc
    26701 aagttattaa ggagtgacag atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga
    26761 agcagtcgaa gcagttgcta atattttgat ggaggcaggg gcgagtggtg tcgcgattga
    26821 agattcttta gatgttgaac attttcagtc ggatcaatat ggtgagattt tagataaaaa
    26881 tgattttaca tcgctgaaag aaggcgcact agtcatggct tattttccgg aaacgacttt
    26941 cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca caattacctg agtttggtct
    27001 agctattgga aaaaataaag taacagttag tgaagtagag gaaagcaact gggcaacagc
    27061 ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg
    27121 ggaaaactac cagacagaag atcctctaga aaaaattatc acactagatc ctggtatggc
    27181 atttggcaca ggcactcatc ccacgacttc cttgacattg acagcactag aaacagtctt
    27241 gcgtggtggt gaaacattgc tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc
    27301 aaaatatctt ggtgcaaaag atgtctatgc ctttgattta gacgaagtag ccgttcgttc
    27361 ggcgaaagaa aatatggata tgaatgaaat agccaaagat gtccatgtat cagctaacga
    27421 tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat
    27481 catcttattg atgatcccag atgcttggcg tttactgaag caaacaggca cgctgatcgt
    27541 ttcaggaatc attgaagaga agaagcaaat ggttctggat gccatgttag aacaagggtt
    27601 tatcgtggat caaattttcc agcaaaaaga ctggtatgca attattttga aaaaaccaga
    27661 ggtggaatag atgcagcggt attttttaaa agaatcatat gaatcaaaag actattttgt
    27721 tttagaaggg gaaaactatc accacattgt acgggttatg aggatggaac ccgaggatga
    27781 agtttttttg gcattcaatg atcaaatagc cattgttgca gaaatcacgg agattactga
    27841 agaggctgtt tatctgaaag aaattaaaaa agaagaaaat caaaaggaac tgccaagttc
    27901 gattaccata gcgagtggct atcctaaagg ggaaaagctg gattggatcg ttcagaaatc
    27961 cactgaatta ggagcgcaag cttttcttgg tttcccggca tcagcctctg tcgttaaatg
    28021 ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa aagattgctc aagaggcagc
    28081 ggaacaatcc catcggcaga tcgtgccatc agtagagtta ttggaaacga aacaagattt
    28141 atttgagcgg ctagatgatt atgacgtggt cttggttgct tatgaggaat cagcaaagca
    28201 aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca aaaccagata gtcgaatctt
    28261 agctgtcttt ggaccagaag gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca
    28321 caaagggata ctttgtggat taggaccaag aatccttcgt acagaaacag cacctcttta
    28381 tttattaagt gctgctagct atcaatggga attaatgaat ccttctttca gctaacgttc
    28441 ttcattaata aaagagtatc gacagaaaga attgaaaaaa tagtcaacaa tttgtataat
    28501 agaaacaatt atttatacaa tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga
    28561 acatttccgt aaaaaaacgt ataactgcac ggattttggg acaattatca aacagatatt
    28621 tgtcccgatt cccgttttac cgaaaaaaga aagggggatc gattcctgtg acaaaagaag
    28681 aaattttaac gggacccggt gtcataaagc tcgttagtca atacatgggt cccgaacatg
    28741 tagcatttgt tgagaaagca tgcgaatacg cgactgctgc acatgacgga cagtttagaa
    28801 aatcaggcga accctacatt attcatccta tccaagtcgc aggtatatta gcagatttaa
    28861 aaatggatcc ccatacagtg gctacaggct tcttacatga tgttgttgaa gatacagaaa
    28921 tcactttaga agatctgaga gaagaatttg gcgatgacgt tgctatgtta gtggacggcg
    28981 taaccaaatt agggaaaata aaatataaat cccacgaaga gcagctagca gaaaaccacc
    29041 gaaagatgct gctagcaatg gctcaagatt tacgagttat catggtcaaa ttagctgata
    29101 gactacataa catgcgtacg ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc
    29161 aggaaacttt agaaatctat gcgcctcttg cacatcgtct agggatcagc cggatcaaat
    29221 gggaattaga agatacagca cttcgttatc taaatccaaa acagtattac cgtatcgtcc
    29281 acttgatgca gacgaagaga gaagaacgtg aaaaatacgt aagtggtact gttgaagata
    29341 ttcgaatagc gacagaagag ctggggattt ttgcagaaat ctatggacgg ccaaaacaca
    29401 tttattcaat ctatcgtaaa atgaaagatc agaaaaagca gttcaacgaa atttatgact
    29461 tgctagctat tcgagtgatc gtagattcaa tcaaggattg ttatgctgta ttaggagcaa
    29521 tccacacaaa atggaaacca atgcccggca gatttaagga ttatattgcc atgccaaaag
    29581 ctaatatgta ccaatctttg catactaccg tgatcggtcc ggcaggaaat ccggtagaaa
    29641 ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt cggggttgct gcacactggg
    29701 cctataaaga aggaaaaaat gaaaaagtag aaccagatgg tatgacgaaa caattaagct
    29761 ggttccatga gatactcgaa cttcaagacg aaagctatga tgcttctgaa tttatggaag
    29821 gcgtaaaagg agatatcttt agtgataaag tctacgtctt cacaccaaaa ggagacgtta
    29881 ctgagttacc aaaaggatcc ggaccattgg actttgcata cagtatccat acagatatcg
    29941 gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt gcagcttgat tacaaattga
    30001 aaaacggaga tatcattgag atcatgactt ctccaaattc atttggccca agtcgcgact
    30061 ggttgaaatt agttgctact agcaaagcaa gaaataagat caaacgtttc ttcaaagccc
    30121 aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt ggtcaaatgt attacagatc
    30181 taggatttac gcctaaagat attttgacga agaacaaact gcaagaagca ctcgatcgtt
    30241 ttaattatca aacagaagat gatctctatg cagctgtagg gtatggagaa gttagcccct
    30301 tgacgatggc caatcgtctg actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa
    30361 agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac
    30421 gtcatgaagg tggcgttgtc attcaagggg tagaaaactt actaattcgt atcagtcgct
    30481 gctgtaatcc gattcctggt gatgatatcg ttggttatat cactaaaggc agagggatat
    30541 ccattcatcg tcgagattgt ccgaatgttc agcctgacaa accaaatgta gcagaacgtt
    30601 tgattgaagt cgaatgggaa gatacatcga atacacgaaa agagtatgat gcagatttgg
    30661 aaatttacgg ctataatcgt tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc
    30721 taacgaaaaa tctcaacagc gttgaagcac ggacgaataa agataaaatg gcgacgatcc
    30781 atttgacggt tggtatccag aatttatccc acctaaagag tatcgtggat aaaatcaaag
    30841 cagtacctga tgtctacagt gtacgccgga cgaatggata ggaggacaga tatgagagca
    30901 gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt
    30961 ggctacggcc tacttgtttt attaggcatt catgaaaccg atacgcaaaa agatgtggac
    31021 tatctggtaa aaaaaattgc acaaatgcgt atttttgaag atgaacaagg aaaaatgaat
    31081 attagtgtag aagatgtggg aggacaaatt ttaagtatct ctcaattcac actgtttgcg
    31141 gatacgaaaa aaggaaatcg accaagcttt gtggaagccg cacgaccaga aacagctatt
    31201 cctttatacg aatcattcaa tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga
    31261 caattcggag cagatatgtc ggtttctctc gtcaatgatg gacctgtaac gatcattatt
    31321 gattctaaaa acaaataaag taatctggtt ttcactagta aaaactatga aacacgtcgc
    31381 ttaagttaga ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt
    31441 ttgtcagcag tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt
    31501 tgctgaagat gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt
    31561 atcaaaaagg atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca
    31621 aagcaaagat tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat
    31681 tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac
    31741 tttttggtca aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata
    31801 tttttatgga tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga
    31861 atctgacaca agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga
    31921 tggttttctt tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag
    31981 atgaggtcga aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc
    32041 cgattgatca cataaaaggt gtcgagaatc gcaaaaaatc agaaaaatgg gcgcaaggct
    32101 tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt
    32161 ttgccaatgc cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat
    32221 tattgaacat catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg
    32281 cacttttcag tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag
    32341 atccgtttat tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt
    32401 cgctgaaaga agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag
    32461 atttatatgg aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca
    32521 aacggtatgt agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac
    32581 aaggcggggg aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata
    32641 gtctacgtgt gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag
    32701 attggacgat tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca
    32761 agaattagat tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct
    32821 tttttcatat tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc
    32881 aatggtttga taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac
    32941 ttgctctgga cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca
    33001 agctaaaaca agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc
    33061 gacagcggtt aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg
    33121 atctccttca ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga
    33181 agcaagagga aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc
    33241 tttttcagcc tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc
    33301 tgcattcgct tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt
    33361 tcctgcttct tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc
    33421 cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc
    33481 caccgattgt ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg
    33541 agcagctttt gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc
    33601 gccaatcagt tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat
    33661 cccgctattc aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa
    33721 aagattatgt cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga
    33781 atgtccgatt cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa
    33841 taccaaaaag ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt
    33901 tgagactggt ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt
    33961 ttttgaaact ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc
    34021 taaagacact ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa
    34081 gaggaaagag aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt
    34141 tcatgatgaa caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg
    34201 agtttccaaa gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag
    34261 tttgtcgaag aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca
    34321 ccgatttttg aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt
    34381 tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa
    34441 ggtacagcac caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg
    34501 aaaccgttca aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga
    34561 cggttacgtc aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact
    34621 gatgtggaga gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc
    34681 cggctagtca tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg
    34741 atcgactatc ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat
    34801 gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa
    34861 gctccatcta tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt
    34921 tccatgctgg atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta
    34981 gattattaca cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa
    35041 gcaacgattt gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca
    35101 acacctggct tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag
    35161 gaagtagtta tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa
    35221 acaaatatcg aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac
    35281 cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc
    35341 aaattagtat tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca
    35401 atggcaagcc gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa
    35461 gtctatgatg aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga
    35521 tctattgcgg attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg
    35581 tacaaaaaag acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa
    35641 tcgttcaaat cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat
    35701 gtcgcagtga atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag
    35761 taaatcctaa aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga
    35821 atcaagcaaa aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac
    35881 gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa
    35941 gaaatgcgac aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg
    36001 aaacacctta cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac
    36061 gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc
    36121 tgatgaatgc tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt
    36181 tacgtggaga ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag
    36241 cagaagaaat ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg
    36301 gtatcgaagt cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg
    36361 gcagcgacaa accggataca cgttttgata tggaattgat cgatctaagc gatacagtca
    36421 aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat
    36481 tgaatgctaa aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat
    36541 atgtaggaca attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga
    36601 aaggtccaat tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg
    36661 caaaaccagg tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat
    36721 taggtgctat ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca
    36781 atttcttatg ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg
    36841 tctctgctca ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag
    36901 accctgcaaa agtctatgca gaagcttatg atatcgtctt gaacggttat gaattaggcg
    36961 gaggttctat ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct
    37021 ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat
    37081 tccctccaca tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag
    37141 aagataatat tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga
    37201 gtgatgcacc aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga
    37261 atatagaaga atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca
    37321 accttgattt aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt
    37381 ccatgaaaaa aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat
    37441 ctatcgttta tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata
    37501 tttattcaag tggtattact ggtctagctc agattttgac gactttgtca aaatcactga
    37561 taggattcga agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat
    37621 tggcgtggtt aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga
    37681 cctccctttg tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg
    37741 cgttgtttgg gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat
    37801 catcgggcgg tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg
    37861 gttctatttc gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct
    37921 ggcaatatat gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg
    37981 tttatactaa acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag
    38041 taaatgaaat ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg
    38101 cttataagca tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc
    38161 tacttagaga agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg
    38221 tccagatact tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat
    38281 gaaatactgc tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc
    38341 agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta
    38401 tttttttaaa tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg
    38461 ctattttcac ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat
    38521 tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc
    38581 cgttcattag ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc
    38641 ggctatttgg gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg
    38701 cggcaggaat gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg
    38761 aagagactct atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg
    38821 tttcagcgag ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc
    38881 aacaactggt ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca
    38941 ccgtttacgc atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc
    39001 ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta
    39061 ggtacatcct tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca
    39121 acccgtgttt attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt
    39181 gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt
    39241 ctgaatatcg ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat
    39301 gatcattaca atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg
    39361 ctagttgcag tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta
    39421 tttacttgga ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt
    39481 ttttatgaag caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa
    39541 atcacattga ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca
    39601 cgttttgatt atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc
    39661 agtaagagca aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt
    39721 ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc
    39781 gctgaaagta aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg
    39841 gttgaccaag cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa
    39901 aaattagaac taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa
    39961 gaactttatc caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta
    40021 ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag acactgttct gattggacta
    40081 ggaacgaatg gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat
    40141 cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat
    40201 gcgatgcttg aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc
    40261 tacagtaatg accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg
    40321 atgacacatt acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac
    40381 agcagtgaga caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt
    40441 ttttgcctga agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc
    40501 aaaacggttg agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat
    40561 gcagcttctg cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta
    40621 acaggtcttc ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct
    40681 aatcgtctag aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat
    40741 acgtaaaaat aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc
    40801 atccatattg taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt
    40861 gattccttca actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat
    40921 ccttttaggt gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg
    40981 acgatggacc atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac
    41041 ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt
    41101 tttatttgtt ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt
    41161 atgtcgaacg acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt
    41221 taaatggtta agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat
    41281 agcgtcgata gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc
    41341 acaaaggtca acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc
    41401 atctaaagaa aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa
    41461 ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg
    41521 tctgtattat actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt
    41581 atgcatacta tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg
    41641 gggtagttcg agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag
    41701 ttttaccgca agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat
    41761 tatatctggc ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt
    41821 tcaaaaatcc agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag
    41881 aaaatccagg accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga
    41941 gtttgatcat gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat
    42001 tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac
    42061 ttggggatct gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat
    42121 cagatatcaa gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag
    42181 cttttgacaa attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat
    42241 aaaatgataa gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact
    42301 ttttcaatga aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta
    42361 aaatagaaga caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt
    42421 ttgaggtgat tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca
    42481 aggttatgac ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa
    42541 agaagaaaaa gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc
    42601 tgttgatgcc gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat
    42661 cccgattttt gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc
    42721 atggttcagt tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt
    42781 cgagtttaaa aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt
    42841 atattttgaa gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa
    42901 aatgcaggag ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat
    42961 gggaagtgta aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc
    43021 tggtgaacta cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc
    43081 tttgagacag gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat
    43141 caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag
    43201 ggagatcgaa cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga
    43261 tcctgccttt tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag
    43321 aacgatcatc ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat
    43381 tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa
    43441 agatcctcaa aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc
    43501 aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag
    43561 aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga
    43621 tttttcacgt ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa
    43681 aacgacgatt tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg
    43741 ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt
    43801 ttcttttgaa catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc
    43861 aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga
    43921 tgggaaagga aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag
    43981 agaactgatg aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga
    44041 attccgtaat tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt
    44101 cggtactcaa tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca
    44161 acaaaaattg gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt
    44221 caaagaagag gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca
    44281 ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa
    44341 acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga
    44401 aaaaacgata ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga
    44461 aaagaaacga tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg
    44521 cctacaagaa ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa
    44581 gaaactagcg atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt
    44641 acgccgacaa acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt
    44701 gccaattgtc gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt
    44761 ggataaagaa gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag
    44821 aactcgaatt cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc
    44881 ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa
    44941 agacactggg tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac
    45001 aaaaaatagt cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg
    45061 ggcaaagcag caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt
    45121 ttgtggatca acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga
    45181 tatcttaaaa gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca
    45241 aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga
    45301 acaaaaaatc atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc
    45361 tttgaaaaag gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat
    45421 cgaacaatct ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga
    45481 aataagacaa caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac
    45541 agaaaaaaat aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt
    45601 gcaagatacg gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga
    45661 agaagactcg aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct
    45721 tcatacgctt cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa
    45781 aatagaaaag gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac
    45841 aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac
    45901 agttacttct tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa
    45961 tatccagcta actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat
    46021 aagacgtatg atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag
    46081 taatcaaaaa atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga
    46141 tgagcttacg caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac
    46201 tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga
    46261 tcatctccaa cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag
    46321 ttatcgaggg aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg
    46381 aagtgcgcat accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc
    46441 tcttgccgat gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga
    46501 tgaaggattt gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac
    46561 gatcgaaagt gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg
    46621 agtattacaa caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca
    46681 acttggataa aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc
    46741 acgtggacgt caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga
    46801 aaacgtgtgg catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac
    46861 agcaaaagag atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca
    46921 tacagttgct gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga
    46981 ccagccaatg ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt
    47041 ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca
    47101 agtagaaacg attgagacta atcagtatca cccagagctt tctattttgg cactttactt
    47161 gcgtatcggt tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt
    47221 tttacaagct tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct
    47281 agataaacgg gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc
    47341 tcagacgcat caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag
    47401 atatctgctt ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac
    47461 acgtttgcat ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa
    47521 caaaccttta tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa
    47581 tgattttgat tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc
    47641 tctgacgaaa tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga
    47701 gaaaccagaa gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc
    47761 tctgctgtca gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga
    47821 tatccccgtc gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt
    47881 cgatttcgtc tatggtgaag tcatctattc gactgacccg aagcatgagg tctcgacaga
    47941 acaggctttt caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag
    48001 aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga
    48061 actatatgcc tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact
    48121 tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt
    48181 aaccgagacc ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga
    48241 aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg
    48301 tcaggttctt tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt
    48361 acgggaatcg atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat
    48421 cattcaaaat caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt
    48481 acaagatctc attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac
    48541 gatgagagat tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt
    48601 tggcggtatt ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt
    48661 actttcggaa aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt
    48721 aatttacaac tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat
    48781 caatgggaat aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac
    48841 ttcttatgcc agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt
    48901 gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag
    48961 agaattagct gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga
    49021 agaattgtgg tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt
    49081 cagggaactt gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga
    49141 taaacaaact gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact
    49201 tacagaagaa caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac
    49261 atcaatggat tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag
    49321 actgcgacaa atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg
    49381 caagctcgaa caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt
    49441 gctgttttct caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg
    49501 gatctcgaca ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga
    49561 cgcatttaat gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg
    49621 attgaatctc acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat
    49681 tgaagaacaa gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg
    49741 gcgtatgatc tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg
    49801 cgaactgttc caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga
    49861 agacatccgc atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt
    49921 tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag
    49981 caaggaggct gggattccat tttatcccag cttccttgct atcatttttt tatttctatt
    50041 tttaaaggtt atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc
    50101 tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct
    50161 tgccagttag cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag
    50221 gtctcttctg gaagcatatg aaattctttc tcagccagct gaaaaatctc tttttctggt
    50281 ttttggaaac ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat
    50341 tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat
    50401 gggacattct tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata
    50461 ttatcaagtt cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga
    50521 gggtagtcta aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc
    50581 caatctcctg ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat
    50641 agtggatgca tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat
    50701 ggctgctgct gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt
    50761 ttacacgcct ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa
    50821 aattgtctaa aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca
    50881 gaataaggtt tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt
    50941 tgatgaacaa attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt
    51001 ggaattttcg aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa
    51061 taacgttatt tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat
    51121 actacgctta tataacagag aaaataagcg ttataataaa gaacagtatt atttaaaatg
    51181 acagaaaaca ttactattaa agaaataaaa aacataaaaa atcttaagaa atcggtttca
    51241 tttagctttt gagataaaat aatagtacag ttttatacgc tgtttcatat gtttagaaaa
    51301 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
    51361 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
    51421 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
    51481 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
    51541 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
    51601 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
    51661 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
    51721 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
    51781 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
    51841 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
    51901 attgatggct ggattataga aggataatac gaaacttgga aaaga
//
LOCUS       DACERL010000014        51806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.014, whole genome shotgun sequence.
ACCESSION   DACERL010000014 DACERL010000000
VERSION     DACERL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51806
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.014"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..98)
                     /locus_tag="IXF54_000793"
     CDS             complement(<1..98)
                     /locus_tag="IXF54_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ1404074.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKV"
     gene            complement(113..1360)
                     /locus_tag="IXF54_000794"
     CDS             complement(113..1360)
                     /locus_tag="IXF54_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1404075.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1436..1582)
                     /locus_tag="IXF54_000795"
     CDS             complement(1436..1582)
                     /locus_tag="IXF54_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1404076.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1686..1997)
                     /locus_tag="IXF54_000796"
     CDS             complement(1686..1997)
                     /locus_tag="IXF54_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1404077.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1999..2196)
                     /locus_tag="IXF54_000797"
     CDS             complement(1999..2196)
                     /locus_tag="IXF54_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1404078.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2590..4317)
                     /locus_tag="IXF54_000798"
     CDS             complement(2590..4317)
                     /locus_tag="IXF54_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1404079.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4310..5503)
                     /locus_tag="IXF54_000799"
     CDS             complement(4310..5503)
                     /locus_tag="IXF54_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1404080.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5690..6334)
                     /locus_tag="IXF54_000800"
     CDS             complement(5690..6334)
                     /locus_tag="IXF54_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1404081.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6428..6562)
                     /locus_tag="IXF54_000801"
     CDS             complement(6428..6562)
                     /locus_tag="IXF54_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1404082.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6564..8696)
                     /gene="feoB"
                     /locus_tag="IXF54_000802"
     CDS             complement(6564..8696)
                     /gene="feoB"
                     /locus_tag="IXF54_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1404083.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8693..9166)
                     /locus_tag="IXF54_000803"
     CDS             complement(8693..9166)
                     /locus_tag="IXF54_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1404084.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9567..9791
                     /gene="nrdH"
                     /locus_tag="IXF54_000804"
     CDS             9567..9791
                     /gene="nrdH"
                     /locus_tag="IXF54_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1404085.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9950..12109
                     /gene="nrdE"
                     /locus_tag="IXF54_000805"
     CDS             9950..12109
                     /gene="nrdE"
                     /locus_tag="IXF54_000805"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1404086.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12159..13124
                     /gene="nrdF"
                     /locus_tag="IXF54_000806"
     CDS             12159..13124
                     /gene="nrdF"
                     /locus_tag="IXF54_000806"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1404087.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13213..14352)
                     /locus_tag="IXF54_000807"
     CDS             complement(13213..14352)
                     /locus_tag="IXF54_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1404088.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14661..15374)
                     /locus_tag="IXF54_000808"
     CDS             complement(14661..15374)
                     /locus_tag="IXF54_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1404089.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15628..16329)
                     /gene="nagB"
                     /locus_tag="IXF54_000809"
     CDS             complement(15628..16329)
                     /gene="nagB"
                     /locus_tag="IXF54_000809"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1404090.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16563..18095
                     /locus_tag="IXF54_000810"
     CDS             16563..18095
                     /locus_tag="IXF54_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1404091.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18286..19488)
                     /locus_tag="IXF54_000811"
     CDS             complement(18286..19488)
                     /locus_tag="IXF54_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1404092.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19570..20259)
                     /locus_tag="IXF54_000812"
     CDS             complement(19570..20259)
                     /locus_tag="IXF54_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1404093.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20365..20733)
                     /gene="rplL"
                     /locus_tag="IXF54_000813"
     CDS             complement(20365..20733)
                     /gene="rplL"
                     /locus_tag="IXF54_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1404094.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20786..21289)
                     /locus_tag="IXF54_000814"
     CDS             complement(20786..21289)
                     /locus_tag="IXF54_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1404095.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(21699..22388)
                     /gene="rplA"
                     /locus_tag="IXF54_000815"
     CDS             complement(21699..22388)
                     /gene="rplA"
                     /locus_tag="IXF54_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1404096.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22492..22914)
                     /gene="rplK"
                     /locus_tag="IXF54_000816"
     CDS             complement(22492..22914)
                     /gene="rplK"
                     /locus_tag="IXF54_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1404097.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23257..23925
                     /gene="sdaAB"
                     /locus_tag="IXF54_000817"
     CDS             23257..23925
                     /gene="sdaAB"
                     /locus_tag="IXF54_000817"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1404098.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23938..24810
                     /gene="sdaAA"
                     /locus_tag="IXF54_000818"
     CDS             23938..24810
                     /gene="sdaAA"
                     /locus_tag="IXF54_000818"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1404099.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24901..25920)
                     /locus_tag="IXF54_000819"
     CDS             complement(24901..25920)
                     /locus_tag="IXF54_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1404100.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26093..26980)
                     /locus_tag="IXF54_000820"
     CDS             complement(26093..26980)
                     /locus_tag="IXF54_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1404101.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27057..27602)
                     /gene="nusG"
                     /locus_tag="IXF54_000821"
     CDS             complement(27057..27602)
                     /gene="nusG"
                     /locus_tag="IXF54_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1404102.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27703..27873)
                     /gene="secE"
                     /locus_tag="IXF54_000822"
     CDS             complement(27703..27873)
                     /gene="secE"
                     /locus_tag="IXF54_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1404103.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27897..28049)
                     /gene="rpmG"
                     /locus_tag="IXF54_000823"
     CDS             complement(27897..28049)
                     /gene="rpmG"
                     /locus_tag="IXF54_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1404104.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28211..28855
                     /locus_tag="IXF54_000824"
     CDS             28211..28855
                     /locus_tag="IXF54_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1404105.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28911..29414)
                     /locus_tag="IXF54_000825"
     CDS             complement(28911..29414)
                     /locus_tag="IXF54_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1404106.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29427..29473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30011..30976
                     /locus_tag="IXF54_000826"
     CDS             30011..30976
                     /locus_tag="IXF54_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1404107.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31075..32247)
                     /locus_tag="IXF54_000827"
     CDS             complement(31075..32247)
                     /locus_tag="IXF54_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404108.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32234..33379)
                     /locus_tag="IXF54_000828"
     CDS             complement(32234..33379)
                     /locus_tag="IXF54_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404109.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33393..34317)
                     /locus_tag="IXF54_000829"
                     /pseudo
     CDS             complement(33393..34317)
                     /locus_tag="IXF54_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34434..35513
                     /locus_tag="IXF54_000830"
     CDS             34434..35513
                     /locus_tag="IXF54_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1404110.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35545..36138
                     /locus_tag="IXF54_000831"
     CDS             35545..36138
                     /locus_tag="IXF54_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1404111.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36248..36475)
                     /locus_tag="IXF54_000832"
     CDS             complement(36248..36475)
                     /locus_tag="IXF54_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1404112.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36578..39166)
                     /locus_tag="IXF54_000833"
     CDS             complement(36578..39166)
                     /locus_tag="IXF54_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1404113.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39186..39845)
                     /locus_tag="IXF54_000834"
     CDS             complement(39186..39845)
                     /locus_tag="IXF54_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1404114.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39894..40397)
                     /gene="trmL"
                     /locus_tag="IXF54_000835"
     CDS             complement(39894..40397)
                     /gene="trmL"
                     /locus_tag="IXF54_000835"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1404115.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40618..41484)
                     /locus_tag="IXF54_000836"
     CDS             complement(40618..41484)
                     /locus_tag="IXF54_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1404116.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41593..42234
                     /locus_tag="IXF54_000837"
     CDS             41593..42234
                     /locus_tag="IXF54_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1404117.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42296..43657)
                     /gene="mgtE"
                     /locus_tag="IXF54_000838"
     CDS             complement(42296..43657)
                     /gene="mgtE"
                     /locus_tag="IXF54_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1404118.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43675..44574)
                     /locus_tag="IXF54_000839"
     CDS             complement(43675..44574)
                     /locus_tag="IXF54_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1404119.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44576..45373)
                     /locus_tag="IXF54_000840"
     CDS             complement(44576..45373)
                     /locus_tag="IXF54_000840"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1404120.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45351..46055)
                     /locus_tag="IXF54_000841"
     CDS             complement(45351..46055)
                     /locus_tag="IXF54_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1404121.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46186..46761
                     /locus_tag="IXF54_000842"
     CDS             46186..46761
                     /locus_tag="IXF54_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1404122.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46879..47556
                     /locus_tag="IXF54_000843"
     CDS             46879..47556
                     /locus_tag="IXF54_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1404123.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47635..49443)
                     /gene="pepF"
                     /locus_tag="IXF54_000844"
     CDS             complement(47635..49443)
                     /gene="pepF"
                     /locus_tag="IXF54_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1404124.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49619..50617)
                     /locus_tag="IXF54_000845"
     CDS             complement(49619..50617)
                     /locus_tag="IXF54_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1404125.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50843..51496)
                     /locus_tag="IXF54_000846"
     CDS             complement(50843..51496)
                     /locus_tag="IXF54_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1404126.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15617 a        10779 c         8970 g        16440 t
ORIGIN      
        1 aatactttat cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt
       61 ataatttgat cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca
      121 acaaaataac ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg
      181 gattcttttt attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg
      241 ttgttattcc ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg
      301 atatcgaaat ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt
      361 gactctctat agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt
      421 caaatattgg aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct
      481 tattgttatt tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta
      541 tattttttaa ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta
      601 attcttttat ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac
      661 tcagcaataa atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca
      721 gtgaatcagt atattttttt agataaacca attcttttct tttcaattgc tgttcatatt
      781 gttctcgttg cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga
      841 ttaaaaatat taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat
      901 aagaaacaaa taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac
      961 tggactgttc aacaatccta tttatagcta attttctata taaccaaata aacatcagca
     1021 aaaacaaaga atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa
     1081 atgaaaacat atacgtcata accatggaag taataataga tctgaaatca tcaataagca
     1141 tacataatag taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa
     1201 aataacttcc gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag
     1261 tgagtggtat caataaacat acaaaaagat attttctttt gttaagatca gtattagtca
     1321 tataaaataa gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg
     1381 tatcgatagt aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt
     1441 tacacttttt aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag
     1501 gttttgttcc agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat
     1561 ttagtctatt tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca
     1621 ctgattcaat atacctattt ttctaatcaa aaaattttga gacatattta atattataag
     1681 tatcattaaa attgagattt atctccataa tctgctcgat ataaaccatt tattccagca
     1741 gttcttccaa tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt
     1801 ttattagtat aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga
     1861 tctaattcat atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata
     1921 tcagaatatt taggatcttt acttatagat atatcattat ataattcatg aacaatttgc
     1981 ttagcatttt ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat
     2041 agacattcct gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt
     2101 gcaatacact ccatttccat aatattttcc actatgagtg gtacccccat agataagttg
     2161 tgtctcttta atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg
     2221 tttttagact attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt
     2281 agcaaaataa ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc
     2341 tttaatattt tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca
     2401 tacctacgat cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt
     2461 tggacgtttc gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg
     2521 aacatggagc ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg
     2581 tctgtctttt tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt
     2641 tgctgcttgc tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt
     2701 ttctgaccaa tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc
     2761 ttttcgaacc atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa
     2821 gtatttcaga tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg
     2881 ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc
     2941 tactttccag tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac
     3001 accagctcct ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat
     3061 cgaatttaca tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc
     3121 acctaggtct tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag
     3181 ccaagctgaa accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt
     3241 atctaatggc gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt
     3301 tgttgaagca acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt
     3361 ggcagatgaa aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat
     3421 gatcagtcct tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt
     3481 tggctggttc aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac
     3541 cccgttgaca aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc
     3601 taagccgggt gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt
     3661 ttgattgatc cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt
     3721 ttgacgactt gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg
     3781 aatccctaaa ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac
     3841 ccctggaacc atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg
     3901 cattgtctgc ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc
     3961 catcaagatc cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag
     4021 tgtgacagta ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat
     4081 taatgtgaac aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa
     4141 taacaagata aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac
     4201 tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc
     4261 tgtgatattt tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt
     4321 cactcacctc gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt
     4381 ttaattttcc atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa
     4441 tatcacttac caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca
     4501 atggacgttt ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt
     4561 gtctctttct gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg
     4621 caacgtttgg tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag
     4681 ccgtaagcac tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg
     4741 gattggtcaa tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg
     4801 cgattctgtc cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt
     4861 tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat
     4921 caagagccga gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg
     4981 ctaggccaac ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg
     5041 aaagcaaact agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta
     5101 caccacggac ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac
     5161 cgaagttttg gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc
     5221 ttaacttcga tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac
     5281 gattcaatag acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga
     5341 tttctccttc ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct
     5401 ttaaaatctc cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt
     5461 taccaaatat ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt
     5521 atgtaaattc atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca
     5581 ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt
     5641 gtaaataaaa ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa
     5701 gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa
     5761 aatgaacgat gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt
     5821 cattatgaga aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa
     5881 ttgaacggtc cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat
     5941 tcatcttatg tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat
     6001 tcagctcatc ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg
     6061 attgtacatc ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg
     6121 ccccacttcc atgccgtact tccatgattc caagatctac cagcacattg atagccttgc
     6181 gggtagtctc aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct
     6241 cacccacttt atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt
     6301 agcgcgggcg gacgattttt ttctttccag acattttctt tcctctttct ttttttactt
     6361 tccctcatct tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa
     6421 cgactgatca tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac
     6481 aattcttacc ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca
     6541 cacttaataa gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac
     6601 tggttcttgg attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata
     6661 gcaagatacg tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc
     6721 acaaaactaa ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc
     6781 ttggcattca tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa
     6841 ttgaaggcta acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag
     6901 atttctctgc cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata
     6961 atattttctt tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa
     7021 ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct
     7081 tcagaaacag gttgagcata aaaattataa gaagagctga accagatcac gatactagaa
     7141 acaaagataa tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt
     7201 aggaccgctg accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta
     7261 tctccagcaa acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata
     7321 ccaagaaaat aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact
     7381 aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa
     7441 cgacgatctt gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct
     7501 gtagcaatca gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga
     7561 tccatgacaa aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa
     7621 aacaatataa tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg
     7681 ttcaagatca gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca
     7741 tgttctggta accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct
     7801 actgtttgaa tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct
     7861 aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca
     7921 tttgacacaa cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga
     7981 atcgtctcac tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct
     8041 ttttgctgag aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat
     8101 ttcattgcat accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt
     8161 aaagctgctt ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt
     8221 gcagactcct gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt
     8281 ggtaaaactg gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct
     8341 tttttgatga catccatcat attcagacca atgatcatag gaatgtctaa atccaataac
     8401 tgcgtagtta aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt
     8461 tcaggctgtt cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac
     8521 aaagaataga tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct
     8581 tctttttttt ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag
     8641 agattgaaca agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt
     8701 tttcctccct ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc
     8761 ctcgcactgt cagttcgatt ggatctccca gaggggctag ttttcggata actattttta
     8821 catttcgcgt caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg
     8881 ctacgacgat cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg
     8941 aagcagtctt tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat
     9001 gactttttac cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc
     9061 cggcaacgat cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt
     9121 cgacttgata aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct
     9181 tgtgaaataa atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac
     9241 gcaaataaat gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat
     9301 acttgtgata ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt
     9361 cttcatagtt ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg
     9421 ttttcagctc ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata
     9481 tgtagtgttt gttgtttgac ttttttacta agagttttat actatctatt atagataata
     9541 agaatgtgtt agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta
     9601 tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca
     9661 atatcgatga acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc
     9721 ctgtcattac ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac
     9781 ttgccagcta acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga
     9841 cagcaccagt ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat
     9901 gatttgcaat caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa
     9961 aaatttacaa gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg
    10021 tcaaatccct ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa
    10081 accaaatacg atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga
    10141 atatatagaa gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca
    10201 atttttggac gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc
    10261 tcaatatgcg ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt
    10321 agccttcaat gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga
    10381 cgaaatgatc catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa
    10441 acgtcggggc gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat
    10501 cgggcgctca atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac
    10561 tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg
    10621 tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca
    10681 gcgtcaagga gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct
    10741 ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt
    10801 gatcgtacct gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag
    10861 tccatatagt gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga
    10921 gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct
    10981 tgaaaatgaa atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga
    11041 tacagcgaac cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga
    11101 gattttacaa gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg
    11161 aacggatatc tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa
    11221 ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga
    11281 tatcgatgtc gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg
    11341 tgccatgggc ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc
    11401 attagacttt acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa
    11461 caatatcgct cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga
    11521 cggcacttat ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa
    11581 agcattgttt gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc
    11641 agtaatgaaa gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat
    11701 ttcatatatc aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg
    11761 ccaagagaag aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat
    11821 tccgtattac acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat
    11881 ggcacaacaa cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc
    11941 agaaggcatt tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt
    12001 aagacattac gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga
    12061 tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata
    12121 gattcgctta ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag
    12181 caataaactg gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag
    12241 agcaattttg gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat
    12301 tgtctcaatt ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata
    12361 ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag
    12421 aagccgtttt gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta
    12481 tttttagtac actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga
    12541 atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc
    12601 tagaaaagaa aattgccagc gtctttttag aaacattctt gttctattca ggtttttata
    12661 ctcctcttta ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa
    12721 tcatccgaga tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca
    12781 acgagttacc tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg
    12841 agctttacga aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag
    12901 aagaagtcaa gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg
    12961 atccactctt tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa
    13021 caggcacaag caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg
    13081 tagaagcgat gaaagacgac gattatttga ttggattgga ataaggattt attttaattc
    13141 aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac
    13201 tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga
    13261 aacatgttcc attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat
    13321 cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc
    13381 aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg
    13441 cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc
    13501 ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc
    13561 gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg
    13621 atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt
    13681 actgctgata taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt
    13741 acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa
    13801 cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga
    13861 aatgattttt gagacacttg cagataaact gactaatatg atattgaaca aattactaag
    13921 agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg
    13981 cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa
    14041 ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat
    14101 gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag
    14161 aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa
    14221 aactgccgaa agcattttca atattggact aaaaatattc ggcatttgta aaataaagta
    14281 cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa
    14341 acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa
    14401 gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa
    14461 tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca
    14521 gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa
    14581 acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct
    14641 attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt
    14701 ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc
    14761 gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct
    14821 tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta
    14881 tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact
    14941 gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg
    15001 atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc
    15061 gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga
    15121 gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca
    15181 tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca tgctcccata
    15241 caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg
    15301 attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat
    15361 accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca
    15421 aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt
    15481 cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca
    15541 aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc
    15601 acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat
    15661 cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc
    15721 ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc
    15781 tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc
    15841 tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt
    15901 cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc
    15961 taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc
    16021 ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa
    16081 taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta
    16141 ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt
    16201 atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat
    16261 accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat
    16321 gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt
    16381 tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat
    16441 aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac
    16501 caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta
    16561 ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg
    16621 aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag
    16681 ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga
    16741 tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa
    16801 cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa
    16861 aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag
    16921 ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg
    16981 ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc
    17041 taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg
    17101 ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc
    17161 agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg
    17221 atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc
    17281 atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc
    17341 atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt
    17401 cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc
    17461 ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat
    17521 tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca
    17581 atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat
    17641 ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc
    17701 cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa
    17761 caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc
    17821 aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg
    17881 gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga
    17941 ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga
    18001 tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc
    18061 ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa
    18121 aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag
    18181 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta
    18241 ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc
    18301 gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac
    18361 aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa
    18421 tgaaccagcc gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct
    18481 tgctgcttca cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc
    18541 gacgtctaat aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa
    18601 agcaataaag cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata
    18661 agaatctgcc agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc
    18721 taatatccct gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa
    18781 cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct
    18841 taatagacgt tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac
    18901 agctgtacta ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat
    18961 tccgactgca cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat
    19021 accagatacc gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat
    19081 gaccttccct cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg
    19141 cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc
    19201 aaaaaaagcg gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg
    19261 acgatccacc acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg
    19321 ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt
    19381 tgttccaatc ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa
    19441 tagtaaaata agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca
    19501 tttcctatct tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg
    19561 ccacacttat tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa
    19621 gacaactaac cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata
    19681 tccgtgaaac agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat
    19741 cacgatcgac aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac
    19801 catagaagat ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt
    19861 catttgtcgg attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa
    19921 gcgatgcata aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat
    19981 cgcacctgtc agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat
    20041 acctggaaca gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc
    20101 atgcgaacga atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg
    20161 gatgtctgtc tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc
    20221 caacgagatc aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct
    20281 atattcatta aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat
    20341 ctcagtgctt ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag
    20401 tttagctttc aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc
    20461 gccatcaact acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt
    20521 gataacttta actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc
    20581 ttcagcagca gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc
    20641 aaattcttct tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa
    20701 ctcagcaacg atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt
    20761 ttttatttaa tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg
    20821 ctttgacagc gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata
    20881 gaagtccttc gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt
    20941 taccttcgat gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca
    21001 tgattttcgc tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga
    21061 atacttcgtc catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt
    21121 tgataacttt catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat
    21181 caactgttaa tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc
    21241 ttacttcatc aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc
    21301 acctcctgaa ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt
    21361 actttgctca accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc
    21421 tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac
    21481 ttcgttcata gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt
    21541 gaatatattc aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc
    21601 ggtacaagtt aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat
    21661 tatttttcag ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca
    21721 tggataccag gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct
    21781 gctgatggtt tagcttttag caatacatca tggattgctg caaagttttc aactaatttt
    21841 tcatcgtcaa atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg
    21901 taagttactt taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta
    21961 ccagttttag ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca
    22021 gtagccatca tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt
    22081 ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct
    22141 ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca
    22201 acagcaccac ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact
    22261 tcaactgttg catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca
    22321 acaggataaa ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt
    22381 ttagccattt tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta
    22441 tatctttcaa tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac
    22501 agtgatcccc atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc
    22561 tgcgtttagg tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt
    22621 cgcaacttta tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa
    22681 tagaacagca gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga
    22741 aataacaacc ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt
    22801 gaatcccatg atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt
    22861 tgctttacct gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca
    22921 tacctcctta agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta
    22981 tctgatctga aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag
    23041 caaatttatt tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac
    23101 gctaaattta ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac
    23161 ttgcgctagc atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt
    23221 acaaagattc ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt
    23281 gtctttgata ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct
    23341 gctagaatcg ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt
    23401 tatctatatg aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt
    23461 ggcggcctgt tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca
    23521 tatgaagccg gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac
    23581 tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc
    23641 ggcggtggca atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt
    23701 actcctacct tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt
    23761 atactttctg agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt
    23821 gaaaatgcca ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga
    23881 ctcaaacagc tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg
    23941 tttttatcga ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg
    24001 atgattgcag ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg
    24061 gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc
    24121 gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac
    24181 tttttaagtg gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc
    24241 aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct
    24301 ggtgtgctga tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc
    24361 ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca
    24421 gaagggggat gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt
    24481 tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat
    24541 atgatgggac tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt
    24601 aatgctttag gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa
    24661 agtgtcattc cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg
    24721 tcgattttca aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt
    24781 gcggaagaga ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga
    24841 ttagttatct tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta
    24901 ttaatccatc aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat
    24961 cgtgatcgac aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc
    25021 tagattcaac ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag
    25081 agctttgaag acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt
    25141 gttgactact tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg
    25201 acgtccacca tgattggata cccagatacc atctgcccct gcagagatag cgattactgc
    25261 atcttctggc gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt
    25321 tttgatgtca tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt
    25381 tccttcgcca ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa
    25441 tggaaattgg aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc
    25501 cgttaaaatg atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa
    25561 tctgtcgtct ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc
    25621 tgcgtctttg attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc
    25681 tactccttta gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg
    25741 cgcttggata atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga
    25801 gtcgattccg cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc
    25861 tttcatcgtc cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat
    25921 gcgctctttt acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc
    25981 attacttgta tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc
    26041 atactaaact tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt
    26101 tccaaataaa tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag
    26161 aggcattttc acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg
    26221 catagaccgc taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta
    26281 gttgaccagc aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt
    26341 aatcttgata gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact
    26401 gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt
    26461 gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca
    26521 tggaatgccc cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac
    26581 aagttttgat ccattctgct tgattccact cgttattttt attatcttca aacaatactt
    26641 ggaccatcgg attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg
    26701 ttcctactgt taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca
    26761 tccctttaaa ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg
    26821 ttttttctat cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat
    26881 ttatcttcgt cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg
    26941 cagttgcacg tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat
    27001 agaaaaaaga aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa
    27061 tcgattttat ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg
    27121 atgttgactt tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca
    27181 gcaaatgcgc cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt
    27241 cgcaggctca taccgataga acgcaagata tgattgactt cttctggaag taatggtgct
    27301 ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca
    27361 acataccatg aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta
    27421 tgaacgattt ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg
    27481 acacggaaaa taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt
    27541 actttgtttt catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc
    27601 attgaaggct cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct
    27661 ttcgcttcat tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc
    27721 aaataagctt tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt
    27781 ttcgatgact actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt
    27841 gatttcatct tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat
    27901 ttcgtttctt tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa
    27961 cgttcgccgc tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa
    28021 caagcgagtg ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact
    28081 tatacttcat aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta
    28141 ttgttttgga taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg
    28201 aggtatagcg atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac
    28261 agagactagt acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt
    28321 gccgatctta gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta
    28381 tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc
    28441 tacaaagttc atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct
    28501 tccttatatt gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac
    28561 gttgcttaat atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat
    28621 tgcttccaca ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag
    28681 cagcattgcc agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt
    28741 gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga
    28801 gaaaaagaat tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa
    28861 aagcgaaaaa agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc
    28921 ttttcaaaat caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg
    28981 ataaactcac ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga
    29041 actaacatat aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa
    29101 cgcatcttta catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat
    29161 gtttggccgc caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg
    29221 atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa
    29281 ctttctgaaa tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac
    29341 gctagataaa gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt
    29401 ttcgtttttt gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat
    29461 gaaccgcggt tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg
    29521 tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg
    29581 ggatatggta taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta
    29641 ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt
    29701 gagcaacctc ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg
    29761 aaccttggaa cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt
    29821 ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa
    29881 ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt
    29941 gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca
    30001 ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa
    30061 tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac
    30121 acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga
    30181 aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat
    30241 catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt
    30301 agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg
    30361 ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa
    30421 agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa
    30481 tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa
    30541 gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt
    30601 attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc
    30661 aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt
    30721 tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt
    30781 ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata
    30841 tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca
    30901 agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca
    30961 gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt
    31021 cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt
    31081 ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat
    31141 tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa
    31201 tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt
    31261 tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac
    31321 cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga
    31381 gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa
    31441 agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac
    31501 cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac
    31561 tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt
    31621 gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata
    31681 agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc
    31741 acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc
    31801 gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg
    31861 tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt
    31921 tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt
    31981 aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc
    32041 cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa
    32101 ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa
    32161 tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa
    32221 tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta
    32281 acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat
    32341 cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga tttaattgac
    32401 caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc atcataccag
    32461 caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc accagattca
    32521 tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac gaccccagag
    32581 cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag ataaaacgaa
    32641 agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct gccaaattag
    32701 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
    32761 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
    32821 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
    32881 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
    32941 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
    33001 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
    33061 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
    33121 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
    33181 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga
    33241 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
    33301 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
    33361 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
    33421 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
    33481 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
    33541 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt
    33601 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
    33661 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
    33721 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
    33781 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
    33841 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
    33901 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
    33961 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
    34021 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
    34081 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
    34141 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
    34201 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
    34261 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
    34321 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
    34381 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
    34441 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
    34501 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
    34561 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
    34621 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
    34681 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
    34741 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
    34801 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
    34861 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
    34921 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
    34981 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
    35041 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
    35101 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
    35161 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
    35221 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
    35281 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
    35341 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
    35401 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
    35461 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
    35521 agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
    35581 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
    35641 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
    35701 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
    35761 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
    35821 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
    35881 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
    35941 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
    36001 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
    36061 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
    36121 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
    36181 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
    36241 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
    36301 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
    36361 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
    36421 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
    36481 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
    36541 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
    36601 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
    36661 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
    36721 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
    36781 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
    36841 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
    36901 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
    36961 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
    37021 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
    37081 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
    37141 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
    37201 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
    37261 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
    37321 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
    37381 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
    37441 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
    37501 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
    37561 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
    37621 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
    37681 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
    37741 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
    37801 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
    37861 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
    37921 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
    37981 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
    38041 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
    38101 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
    38161 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
    38221 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
    38281 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
    38341 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
    38401 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
    38461 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
    38521 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
    38581 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
    38641 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
    38701 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
    38761 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
    38821 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
    38881 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
    38941 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
    39001 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
    39061 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
    39121 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
    39181 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
    39241 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
    39301 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
    39361 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg
    39421 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
    39481 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
    39541 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
    39601 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
    39661 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
    39721 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
    39781 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
    39841 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
    39901 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
    39961 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
    40021 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
    40081 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
    40141 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
    40201 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
    40261 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
    40321 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
    40381 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
    40441 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
    40501 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
    40561 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
    40621 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
    40681 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
    40741 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
    40801 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
    40861 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
    40921 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
    40981 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
    41041 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
    41101 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
    41161 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
    41221 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
    41281 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
    41341 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    41401 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    41461 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    41521 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    41581 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    41641 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    41701 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    41761 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    41821 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    41881 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    41941 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    42001 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    42061 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    42121 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    42181 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    42241 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    42301 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    42361 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    42421 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    42481 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    42541 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    42601 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    42661 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    42721 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca
    42781 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    42841 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    42901 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    42961 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    43021 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    43081 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    43141 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    43201 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    43261 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    43321 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    43381 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    43441 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    43501 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    43561 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    43621 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    43681 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    43741 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    43801 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    43861 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    43921 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    43981 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    44041 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    44101 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc
    44161 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    44221 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    44281 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    44341 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    44401 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    44461 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    44521 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    44581 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    44641 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    44701 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    44761 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    44821 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    44881 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    44941 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    45001 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    45061 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    45121 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    45181 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    45241 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    45301 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    45361 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    45421 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    45481 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    45541 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    45601 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    45661 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    45721 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    45781 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    45841 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    45901 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    45961 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    46021 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    46081 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    46141 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    46201 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    46261 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    46321 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    46381 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    46441 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    46501 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    46561 cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    46621 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    46681 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    46741 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    46801 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    46861 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    46921 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    46981 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    47041 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    47101 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    47161 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    47221 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    47281 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    47341 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    47401 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    47461 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    47521 tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa
    47581 gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa
    47641 acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc
    47701 aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc
    47761 gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag
    47821 tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta
    47881 attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac
    47941 tgctggacca taatattttg cattcaattt accataacta tcacttaggt attcactagt
    48001 caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt
    48061 ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac
    48121 acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga
    48181 agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt
    48241 gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt
    48301 atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc
    48361 gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac
    48421 gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc
    48481 ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg
    48541 cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa
    48601 atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct
    48661 caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt
    48721 tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa
    48781 tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac
    48841 gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc
    48901 tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc
    48961 aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat
    49021 ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc
    49081 tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag
    49141 actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt
    49201 aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat
    49261 cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt
    49321 taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt
    49381 ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc
    49441 catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac
    49501 atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt
    49561 agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact
    49621 attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt
    49681 catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt
    49741 tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa
    49801 cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca
    49861 aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt
    49921 gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca
    49981 tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag
    50041 tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca
    50101 aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat
    50161 agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa
    50221 gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct
    50281 ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc
    50341 tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc
    50401 aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg
    50461 aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa
    50521 tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa
    50581 aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta
    50641 cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta
    50701 ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg
    50761 cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac
    50821 agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac
    50881 gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag
    50941 caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc
    51001 agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact
    51061 ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg
    51121 cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa
    51181 taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt
    51241 gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg
    51301 gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt
    51361 ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg
    51421 aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa
    51481 tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt
    51541 gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa
    51601 accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg
    51661 aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat
    51721 cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg
    51781 gaaaaactat ctttgtaata gtcttt
//
LOCUS       DACERL010000015        48740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.015, whole genome shotgun sequence.
ACCESSION   DACERL010000015 DACERL010000000
VERSION     DACERL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48740
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.015"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(23..2095)
                     /locus_tag="IXF54_000847"
     CDS             complement(23..2095)
                     /locus_tag="IXF54_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1404127.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IXF54_000848"
     CDS             complement(2028..2405)
                     /locus_tag="IXF54_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1404128.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IXF54_000849"
     CDS             complement(2534..3094)
                     /locus_tag="IXF54_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1404129.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IXF54_000850"
     CDS             3244..4536
                     /locus_tag="IXF54_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1404130.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IXF54_000851"
     CDS             complement(4596..5714)
                     /locus_tag="IXF54_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1404131.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IXF54_000852"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IXF54_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1404132.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IXF54_000853"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IXF54_000853"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1404133.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IXF54_000854"
     CDS             8920..9519
                     /locus_tag="IXF54_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1404134.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IXF54_000855"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IXF54_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1404135.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IXF54_000856"
     CDS             complement(11214..11558)
                     /locus_tag="IXF54_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1404136.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IXF54_000857"
     CDS             complement(11695..12831)
                     /locus_tag="IXF54_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1404137.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IXF54_000858"
     CDS             complement(13020..13994)
                     /locus_tag="IXF54_000858"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1404138.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IXF54_000859"
     CDS             complement(14074..14949)
                     /locus_tag="IXF54_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1404139.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IXF54_000860"
     CDS             15079..16986
                     /locus_tag="IXF54_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1404140.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IXF54_000861"
     CDS             complement(17527..20805)
                     /locus_tag="IXF54_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1404141.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IXF54_000862"
     CDS             complement(21076..21621)
                     /locus_tag="IXF54_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1404142.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IXF54_000863"
     CDS             complement(21633..22325)
                     /locus_tag="IXF54_000863"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1404143.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IXF54_000864"
     CDS             complement(22344..22628)
                     /locus_tag="IXF54_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404144.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IXF54_000865"
     CDS             complement(22641..23204)
                     /locus_tag="IXF54_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1404145.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IXF54_000866"
     CDS             23432..24094
                     /locus_tag="IXF54_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1404146.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IXF54_000867"
     CDS             24218..25414
                     /locus_tag="IXF54_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1404147.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IXF54_000868"
     CDS             complement(25515..26891)
                     /locus_tag="IXF54_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1404148.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IXF54_000869"
     CDS             complement(26928..27794)
                     /locus_tag="IXF54_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1404149.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IXF54_000870"
     CDS             complement(27883..28908)
                     /locus_tag="IXF54_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1404150.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IXF54_000871"
     CDS             complement(28926..29600)
                     /locus_tag="IXF54_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1404151.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IXF54_000872"
     CDS             complement(29784..30677)
                     /locus_tag="IXF54_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1404152.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IXF54_000873"
     CDS             complement(30751..32448)
                     /locus_tag="IXF54_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1404153.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IXF54_000874"
     CDS             complement(32441..33754)
                     /locus_tag="IXF54_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1404154.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37791)
                     /locus_tag="IXF54_000875"
     CDS             complement(33754..37791)
                     /locus_tag="IXF54_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1404155.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37804..38262)
                     /locus_tag="IXF54_000876"
     CDS             complement(37804..38262)
                     /locus_tag="IXF54_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1404156.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38560..39078)
                     /locus_tag="IXF54_000877"
     CDS             complement(38560..39078)
                     /locus_tag="IXF54_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1404157.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(39080..39697)
                     /locus_tag="IXF54_000878"
     CDS             complement(39080..39697)
                     /locus_tag="IXF54_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1404158.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39694..40548)
                     /locus_tag="IXF54_000879"
     CDS             complement(39694..40548)
                     /locus_tag="IXF54_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404159.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40545..41456)
                     /locus_tag="IXF54_000880"
     CDS             complement(40545..41456)
                     /locus_tag="IXF54_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404160.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41497..42831)
                     /locus_tag="IXF54_000881"
     CDS             complement(41497..42831)
                     /locus_tag="IXF54_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404161.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            43039..43869
                     /locus_tag="IXF54_000882"
     CDS             43039..43869
                     /locus_tag="IXF54_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1404162.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(44113..44362)
                     /locus_tag="IXF54_000883"
                     /pseudo
     CDS             complement(44113..44362)
                     /locus_tag="IXF54_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44553..44747)
                     /locus_tag="IXF54_000884"
     CDS             complement(44553..44747)
                     /locus_tag="IXF54_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404163.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45252..45437)
                     /locus_tag="IXF54_000885"
     CDS             complement(45252..45437)
                     /locus_tag="IXF54_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404164.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46287..46628)
                     /locus_tag="IXF54_000886"
     CDS             complement(46287..46628)
                     /locus_tag="IXF54_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1404165.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46628..46858)
                     /locus_tag="IXF54_000887"
     CDS             complement(46628..46858)
                     /locus_tag="IXF54_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1404166.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            47145..47483
                     /locus_tag="IXF54_000888"
     CDS             47145..47483
                     /locus_tag="IXF54_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404167.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47688..47927)
                     /locus_tag="IXF54_000889"
     CDS             complement(47688..47927)
                     /locus_tag="IXF54_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1404168.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47903..48250)
                     /locus_tag="IXF54_000890"
     CDS             complement(47903..48250)
                     /locus_tag="IXF54_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1404169.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14161 a        10391 c         8537 g        15651 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgcggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacggaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttatt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttattg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt caacttctcc
    37561 gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac gctccaaaat
    37621 accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta cgattggttc
    37681 gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat cttctgtatc
    37741 agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca cttgaagagg
    37801 tagttaccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg tgccggtttc
    37861 tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg tctcgacttt
    37921 taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt tttttacggt
    37981 accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat tggcactttc
    38041 ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat acttcccttc
    38101 aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg ttgactcagc
    38161 agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa atagcaaact
    38221 gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat actcctttca
    38281 aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat tataatttaa
    38341 tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaaacacc aattatacaa
    38401 catttattat taaaattaaa ttataaaatt ttataacgtt ctcatttaac aaaacttaag
    38461 ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa
    38521 tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg cagcgcatag
    38581 gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag
    38641 taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata ttcctgcaac
    38701 tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat gtcctgaagg
    38761 aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt tttcataagg
    38821 tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat agatcaaact
    38881 gccgataaga atggctagac catattctct agtatcaggt atcaagacta aaacagcagc
    38941 aaggagcaac caaataaaac caccattaga gatattactg ataaatttca aaaaattgat
    39001 ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat catcgatttt
    39061 agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc gtacgatgga
    39121 taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca aaatagattg
    39181 gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc
    39241 ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct ttttgttcca
    39301 gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag
    39361 tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga taatcgatca
    39421 atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga gagaagttct
    39481 cacgaaactt tttccagaat agtgaaccga actttttctc ttcatttttt ttgtaatgat
    39541 aaatcatata cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca
    39601 ggaaccatag tagctgtaaa tggataagac gtggaatcca ttcaatgatt tctaagaaat
    39661 aatcattggc ttttttacca atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc
    39721 catcccttca atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac
    39781 aagtgttgct cctgccatca gataattcca ttgtgtcgtt gcttggttct gaaaaacttg
    39841 caagccaatc tgtaacgtgt atttttcttg atcttggata tataacagag gacctaagaa
    39901 atcattccaa gctgcgttga aggaattgat cgcaatggta atcaaggcag gtttcgtcaa
    39961 tggaagcatc aaatgactcc aaatatacaa atgattcgct ccgtcgattt cagcagcttc
    40021 gatcaattca ttattgatcg ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt
    40081 gccgaagaac gatggaacga tcaggggcaa ataagtacct acccagccga tcttggagaa
    40141 taatacatat tgagggatca tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa
    40201 agcaaacatc agctttttcc caggaaaatc aagcttcgca aatccataag caataaaaga
    40261 atttgaaagg acattaccga tcactactag cacagtgatg aataatgtat ttcttgcata
    40321 gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt tcccatacga ttttttctgg
    40381 gaaaaaggtc ggaggataac gaacgatttc ttccattggt ttcaatgaag taaacaccat
    40441 ccaaagcaat ggaatgatca aaatgattcc tattactgtc atgaggacat aacttagtat
    40501 tttttttgtt tgccgattca cactggtctt tttgaaataa tccatcattt gccgtcacct
    40561 ccagaataat agacccactt aggtgcaagc ttcaaattga caaacgtcag aaccaacacg
    40621 atcagaaaca ggatcattga catagccatc gcataaccca tatcaaaagc tacgaacgcc
    40681 ttgttccaca tatgcagatt ataaaaaagc aaagaatttg caggtccgcc tgttccattt
    40741 ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca
    40801 tcgtaaaaaa taataggagt gattaatggc aaagtaatac ttttgaactt tccccaaacg
    40861 cctgctccat cgatttctgc tgattcatat aaagatctag gaacattttg taatgtagct
    40921 aagtaaagta gcattgctcc tccagaactc catattttca tcaaaatcaa cgcaggtttc
    40981 gtccattgtg gatcaaacag ccaagctggt ccagggatat ggaaccagcc cagaaatgtg
    41041 ttgatcaatc ctgcagatgg agaaagcagc tgcatccata agatgtagac tgctactcct
    41101 gataagactg ccggtaaata gaaagctgta cggaatacac tgattccttt gattttttga
    41161 ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt gaagatgacg
    41221 aaataaacgg tgttgtataa cgatttccaa aataaatcat cgtgaacgaa cattcttttg
    41281 aaatttgcta gaccaatgaa attcatcttg gatgtcaaat tataatcggt gaaactacca
    41341 ataactgaaa aaagcatagg ccctagcata aataccaaga acccgataat aaaaggcgag
    41401 ataaataaat aggcccatct tgcttctttt ttctttcgga tagataaacg cttcaaattt
    41461 tcttgcctcc tctgtaccta gacagtcaaa aatggattat tgattttgct tgactaagtt
    41521 ttctactgct ttttgtgcat ctgccaatcc ttcttttggt gattttgttc ctaaaagaat
    41581 ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt
    41641 gatcaccgta tttttaaagt tttcgtccgc catttgatag atcatttttc ctttatccgt
    41701 taggttgtca ttattcacta aattctcatt ggctgtcttg ctcgccatga tatcaaaact
    41761 tttttcgccg aatttttctt gtacttctgg agaagaaaga tatttgatga attcataaga
    41821 ggcctcagga tcttttgctc cgtatggtac ttccaagacg aatccgccac cccacgacca
    41881 atggcctgaa ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg
    41941 tgcattttcc atcaaactag agtaataatt gatattttgg gcacgcatgg ctactaatcc
    42001 ggaaataaat ggatcagata caccagaccc aaattcggct tctaatttat tgatcgtatt
    42061 tttcccataa taatcttgcc agtcttggat ccactccaag gcttccactt tatttttagt
    42121 atcaatcttg actttttcat ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc
    42181 ccatacatca gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa attcttttcc
    42241 ttctttttta tctagtttac gtgctgtttc ttctaattca tcccaagttg tcggtagatc
    42301 ttgttcactg attccagctt ctttaaataa ctttttatta taaaaaatga cttgtgtatc
    42361 tgtattgaat gggatcgcat aaggatcacc ttcatattct actgtatccc aaagctgcgg
    42421 ataaaaatcc ttagaagtgt ctttttctac atatttggag agattagttg cttgctgtgc
    42481 ttctgctctt tgggcaacgg aatttatgtc ctgtacaata acatcgggtg catttccagc
    42541 tgttattgca gataaagatt tagtccagat gtcaccccat ggctgatact tataatttac
    42601 atggatctcg ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag cttcttttcg
    42661 tgcaccggat ccccaaaagg accagaaatc aatatttttt cttccatcac ttgttttttc
    42721 agaagaattt gctgcacttg aactggcatc gcttgcatta cttgatccgc atcctcctaa
    42781 taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat aattttttca tattttacct
    42841 ccaaatttta atgtaaccga tgccggtttt aatataacga tatcattttg ttctgaaaat
    42901 aacaatttat aaggtatgat tctaaaaaaa ttattaatga taccaatact ttatataatt
    42961 cgcgagcgct ttctttgaat tttttaatct ttcgtagcag aatgagaatg tattaaaaat
    43021 agattaggag gaattttgat gtttagacac cagaaagaat tgcagtttga agcaaaacca
    43081 gatcgtccga atccattaat cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc
    43141 gagatgagtg tagctatgca gtacctgttc caaggctgga gctgccgagg agaagaaaaa
    43201 tataaagata tgctgatgga cattgcaaca gaagaaatcg gccacgtgga aatgttggcg
    43261 acaatggttg ctcgtttatt agagggtgcg cctgttgaag atcaagaagc agcgatgcaa
    43321 aaagatccaa caatcggtgc cattatctct ggaatgaatc cacagcacgc gatcgtagct
    43381 ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt
    43441 gcgagtggca acttgttagc agactttaga gctaatttaa atgccgaatc acaaggaaga
    43501 cttcaagtag cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct
    43561 gttctccttg caagagatag ctatcatcaa aatctatggg aatgtgcagt gaaagaatta
    43621 gaagaaaaag aaggttcaat cttagtccca agtactttcc cgcgtgacaa agaacgaaaa
    43681 gacattgctt atgacttcta taacttctca gagggagacc aaagtagtga aggttcttgg
    43741 gctagtggca aagcactgga tggcgaaggc gaatatcaat ggttgaaaga acctaagatc
    43801 gaaggcgctg caccaaaatt agaaccagta acaccaaaaa tgtatgggac acctccattc
    43861 aagaaataaa tatctacccg tagaaacacc cgttttttgt tccacacgct ttgtgagcaa
    43921 aactgactaa agtcattaat cataaccacc cttcaaaagg gtggttttct ctagagctat
    43981 aagctcaaat caccggccag cgcctaaaga cgctggcttt catattttat gttcaagcca
    44041 tattgccttt gccagttttg cccacctctt aaaaggttta tgtactactc cttagccctt
    44101 aaaaggatcg tcttactctc gtacactcag cttatctaac gcactatcat gtttttcttg
    44161 ttcttgaatg tactttttga tggtcgcttc attttgtcct acttttgtca catagtatcc
    44221 ttccacccaa aagtgtcgat acccaaattt atattttaga tttgcacaat ctcttgatga
    44281 tctcctgaat attggctcga tattgattat aaattatttt tcttctatac tttggtgtga
    44341 atacaatgtg gtatttacac atctactttg tacgggctaa actatttccc tttattgctc
    44401 cacccgcata gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt aggtctaaag
    44461 acaataaatc taaaaacccg ctgcttcact gacagaaaca acgggttttc agaaaaaaat
    44521 gaaatgaaaa agatctctta cggcaatcta ttttattttt ttgatttttt ataaatggaa
    44581 aatgcttcaa gaagtccaag aaataaagcc gtataaaaac aagaacctat gaaatcttta
    44641 tttattagat attcaacaga aatcccgata acactagcca acaatatatt tatcagaagt
    44701 gtaaaataag gatgcttttt atagaataga actagtgttg ttttcataga ctctcctttc
    44761 ttcatatttc caattaatca caaattcacg gcattactag aaaaattaaa ggggcgcaga
    44821 agtggaaaaa tactcttcga ctttccctac aaaccaggcc atcttgtgtt taataactat
    44881 accaaatcta aggtacctct aaatctaaat tccatttgtg gcggtgacct ccaattttta
    44941 actcttctat ctcccatcca gtatacataa ccttgatact tcgaccactt atatatcgaa
    45001 cacacaaata cctatccatt gcaggagtac tacctaactt agttgctact tgcatctaaa
    45061 taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac
    45121 tatcattttt atcctcaact acatgcacat ctgttgcaac ttcactttct gtctcatcca
    45181 gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc
    45241 taaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac
    45301 aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat
    45361 gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct
    45421 ttagaatttt tactcattca tagacacttc cttaaataac ttttattata gaaggtattg
    45481 taaataataa tattgacaaa aagtgactca aaataaaaaa aaccttacat tatactggaa
    45541 tgaactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
    45601 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
    45661 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
    45721 cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt
    45781 agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct
    45841 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
    45901 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
    45961 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
    46021 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
    46081 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
    46141 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac
    46201 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
    46261 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
    46321 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
    46381 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
    46441 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
    46501 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
    46561 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
    46621 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
    46681 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
    46741 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
    46801 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
    46861 tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg
    46921 taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa
    46981 attgtagcca gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat
    47041 ggatccaaaa gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata
    47101 aaacgctttg ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac
    47161 aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag
    47221 caggtgaaga agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg
    47281 ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt
    47341 ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc
    47401 ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat
    47461 tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc
    47521 agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga
    47581 tgcagtatat gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg
    47641 aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc
    47701 attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc
    47761 caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact
    47821 gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta
    47881 cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt
    47941 tcttcattcc actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct
    48001 ttttttcttc tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg
    48061 taaatcccca cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca
    48121 caacaagatt tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt
    48181 tcgtctgggc ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac
    48241 gaccacacat cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc
    48301 gcaccaacaa aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact
    48361 ctccagtgaa tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
    48421 atgagaagct gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga
    48481 cctctgggta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
    48541 atgtagaaag ctattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac
    48601 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
    48661 aaaaatatga gcagctattt ttatttggct gttcttatta cttgatgcag ggcagctttt
    48721 tcacaacctc tccactaaac
//
LOCUS       DACERL010000016        44325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.016, whole genome shotgun sequence.
ACCESSION   DACERL010000016 DACERL010000000
VERSION     DACERL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44325
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.016"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(38..1189)
                     /locus_tag="IXF54_000891"
     CDS             complement(38..1189)
                     /locus_tag="IXF54_000891"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1404170.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IXF54_000892"
     CDS             complement(1202..1639)
                     /locus_tag="IXF54_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1404171.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IXF54_000893"
     CDS             complement(1654..3420)
                     /locus_tag="IXF54_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1404172.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IXF54_000894"
     CDS             3684..4361
                     /locus_tag="IXF54_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404173.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IXF54_000895"
     CDS             4364..5491
                     /locus_tag="IXF54_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404174.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IXF54_000896"
     CDS             complement(5691..6131)
                     /locus_tag="IXF54_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1404175.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IXF54_000897"
     CDS             complement(6142..8175)
                     /locus_tag="IXF54_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1404176.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IXF54_000898"
     CDS             complement(8177..9880)
                     /locus_tag="IXF54_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404177.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IXF54_000899"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IXF54_000899"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1404178.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IXF54_000900"
     CDS             complement(10963..11988)
                     /locus_tag="IXF54_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1404179.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IXF54_000901"
     CDS             complement(12197..13066)
                     /locus_tag="IXF54_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1404180.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13858..14412)
                     /locus_tag="IXF54_000902"
     CDS             complement(13858..14412)
                     /locus_tag="IXF54_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1404181.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(14538..16467)
                     /locus_tag="IXF54_000903"
                     /pseudo
     CDS             complement(14538..16467)
                     /locus_tag="IXF54_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(16773..17489)
                     /locus_tag="IXF54_000904"
     CDS             complement(16773..17489)
                     /locus_tag="IXF54_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1404182.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(17490..19313)
                     /gene="glpO"
                     /locus_tag="IXF54_000905"
     CDS             complement(17490..19313)
                     /gene="glpO"
                     /locus_tag="IXF54_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1404183.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(19327..20823)
                     /gene="glpK"
                     /locus_tag="IXF54_000906"
     CDS             complement(19327..20823)
                     /gene="glpK"
                     /locus_tag="IXF54_000906"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1404184.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(20959..22419)
                     /locus_tag="IXF54_000907"
     CDS             complement(20959..22419)
                     /locus_tag="IXF54_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1404185.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(22409..23704)
                     /locus_tag="IXF54_000908"
     CDS             complement(22409..23704)
                     /locus_tag="IXF54_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1404186.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(24036..24713)
                     /locus_tag="IXF54_000909"
     CDS             complement(24036..24713)
                     /locus_tag="IXF54_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1404187.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(24743..25834)
                     /locus_tag="IXF54_000910"
     CDS             complement(24743..25834)
                     /locus_tag="IXF54_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1404188.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(25935..26369)
                     /locus_tag="IXF54_000911"
     CDS             complement(25935..26369)
                     /locus_tag="IXF54_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1404189.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(26953..28410)
                     /gene="ascB"
                     /locus_tag="IXF54_000912"
     CDS             complement(26953..28410)
                     /gene="ascB"
                     /locus_tag="IXF54_000912"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1404190.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(28407..30302)
                     /locus_tag="IXF54_000913"
     CDS             complement(28407..30302)
                     /locus_tag="IXF54_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404191.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(30360..32135)
                     /locus_tag="IXF54_000914"
     CDS             complement(30360..32135)
                     /locus_tag="IXF54_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1404192.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(32185..33681)
                     /locus_tag="IXF54_000915"
     CDS             complement(32185..33681)
                     /locus_tag="IXF54_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1404193.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(33875..35497)
                     /locus_tag="IXF54_000916"
     CDS             complement(33875..35497)
                     /locus_tag="IXF54_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1404194.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(35825..37138)
                     /locus_tag="IXF54_000917"
     CDS             complement(35825..37138)
                     /locus_tag="IXF54_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1404195.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(37383..37607)
                     /locus_tag="IXF54_000918"
     CDS             complement(37383..37607)
                     /locus_tag="IXF54_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404196.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(37627..37995)
                     /locus_tag="IXF54_000919"
     CDS             complement(37627..37995)
                     /locus_tag="IXF54_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1404197.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(38191..38901)
                     /locus_tag="IXF54_000920"
     CDS             complement(38191..38901)
                     /locus_tag="IXF54_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404198.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(38919..39416)
                     /locus_tag="IXF54_000921"
     CDS             complement(38919..39416)
                     /locus_tag="IXF54_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1404199.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(39453..39905)
                     /locus_tag="IXF54_000922"
     CDS             complement(39453..39905)
                     /locus_tag="IXF54_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1404200.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(40040..40645)
                     /locus_tag="IXF54_000923"
     CDS             complement(40040..40645)
                     /locus_tag="IXF54_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1404201.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(41095..41715)
                     /locus_tag="IXF54_000924"
     CDS             complement(41095..41715)
                     /locus_tag="IXF54_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1404202.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(41717..42586)
                     /locus_tag="IXF54_000925"
     CDS             complement(41717..42586)
                     /locus_tag="IXF54_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1404203.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(42609..43016)
                     /locus_tag="IXF54_000926"
     CDS             complement(42609..43016)
                     /locus_tag="IXF54_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1404204.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(43035..44324)
                     /locus_tag="IXF54_000927"
     CDS             complement(43035..44324)
                     /locus_tag="IXF54_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1404205.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12542 a         9464 c         7450 g        14869 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa
    13381 aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg
    13441 attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa
    13501 aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg
    13561 gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    13621 gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc
    13681 agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa
    13741 gaaataattt caaaaacaac ttctcctgtt tttggttcgg ttggcttatt atcgagaagt
    13801 agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta
    13861 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
    13921 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
    13981 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
    14041 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
    14101 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
    14161 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
    14221 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
    14281 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
    14341 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
    14401 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
    14461 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat
    14521 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
    14581 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
    14641 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
    14701 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
    14761 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
    14821 tattcattga caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc
    14881 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
    14941 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
    15001 atccacaaag cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag
    15061 tgcttcccga tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt
    15121 cttgatttct ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc
    15181 catgattgcg atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc
    15241 taaaagtacc aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg
    15301 accagaaaat gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat
    15361 ctcatcaaag acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc
    15421 catcaaagtc aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc
    15481 ggtgataatc aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc
    15541 aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg
    15601 tgtgcttcca ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt
    15661 gatcgtttcg atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc
    15721 cgccaggact tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt
    15781 caatttagaa atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat
    15841 aatcgttcca tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc
    15901 tttttgaaca atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt
    15961 cagtaatttt gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa
    16021 attacgtgac gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc
    16081 tacatttatc aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg
    16141 attcttatca aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat
    16201 caaatcggtt ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct
    16261 cggcaacccc ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt
    16321 ttcttcttga cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg
    16381 ttttttgcta tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg
    16441 taattgacta atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc
    16501 aaaataaata agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt
    16561 cctatcctta ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca
    16621 ccaaataaac tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca
    16681 aaacataccc cattcttcta ggtcttattt attttccact taactatagc aagacacatt
    16741 ttggttgtca aactttagta tgttttatag atttacacca ttaatgaata gacacctacc
    16801 gcgataattg ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa
    16861 gttcctttat tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca
    16921 gggttgattg catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca
    16981 cctacagcga atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca
    17041 agaactaata caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata
    17101 gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt
    17161 ggcaaataag aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg
    17221 atgaaaggaa cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact
    17281 gcaggattca aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg
    17341 agtccccaac caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa
    17401 ttcacagccg cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc
    17461 cccatgattt gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct
    17521 gattctgcaa tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat
    17581 tcaaaatatc cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt
    17641 tcaaacaaca gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct
    17701 tcttctaaac cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc
    17761 atttcttttg ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa
    17821 gtagcatctt cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt
    17881 tcttctaatt tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt
    17941 tctttcgtct ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct
    18001 tctgccatct tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt
    18061 tctctttcta aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt
    18121 tccaaatggt tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca
    18181 cttactgtat cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca
    18241 ttgtaatcag aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg
    18301 atatcattca atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa
    18361 tctacatctt cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc
    18421 gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt
    18481 cctgtatcga agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg
    18541 atccctttag ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact
    18601 ttatctaccc aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca
    18661 tctgtcagca ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca
    18721 accgctttca ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca
    18781 atcactaaac gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct
    18841 tcttttttca aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca
    18901 tatttggttc ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag
    18961 aacatattga aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt
    19021 tttgggatgt gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact
    19081 acttccacat caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga
    19141 gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct
    19201 gctgactgga ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat
    19261 tcttcttgct gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt
    19321 tcctccttat tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt
    19381 ttctcggcgc tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg
    19441 gatctcgtct gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag
    19501 cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa
    19561 ttgcatcaga tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat
    19621 tccagtgtca tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa
    19681 agtagcttta atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg
    19741 tgctgcctga tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc
    19801 atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg
    19861 aagtccatct ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc
    19921 atagtaaacc tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa
    19981 ttgcggtttt tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac
    20041 catgcctggt tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc
    20101 tgaaattggt gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga
    20161 gttagaagtg acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg
    20221 atcccaatct agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt
    20281 aacatgtgct tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat
    20341 caattcgcct ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat
    20401 cttcgttgct gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg
    20461 atagccttca ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac
    20521 tgcatgatag attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt
    20581 cgttatgcca atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc
    20641 aataactgac tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg
    20701 cgggaaatat tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa
    20761 gataattgct cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt
    20821 cattgtgatt ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg
    20881 ttttaaaaaa agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat
    20941 tatgaagcta ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa
    21001 tcgaataatg tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt
    21061 tctctggcaa aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc
    21121 aataccgccg attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat
    21181 cggcagactt tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat
    21241 caaaagaaca ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt
    21301 cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg
    21361 cccgagcatt ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc
    21421 aacaaagaaa aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc
    21481 tttgattttc aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc
    21541 taaaacagcg gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc
    21601 gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag
    21661 ttctgctgct gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg
    21721 gtccgactga tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa
    21781 tagtcttttt ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa
    21841 cgttgtttct aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg
    21901 caaaaaaagc ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg
    21961 tatctgtagc tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa
    22021 gagctggttc aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt
    22081 cgctaatcga acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt
    22141 caagctttcc tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac
    22201 aagcagcgcc tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat
    22261 caattcaata tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct
    22321 caaaccattg atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg
    22381 taactgctct tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg
    22441 accacaccat atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact
    22501 gttcaaacgt ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct
    22561 cttttttgct ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga
    22621 gtttaacttt gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt
    22681 tttggggata aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa
    22741 accactgatt gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg
    22801 tcttgctgac atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt
    22861 catttgttgg acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag
    22921 acgtttgcaa gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt
    22981 cccacatctg attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct
    23041 ttcttttttc cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt
    23101 ctaaaaaaag tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag
    23161 gatctagata acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc
    23221 ttgatgccaa cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt
    23281 tctttgcatt catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt
    23341 gaataagcgt tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa
    23401 tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc
    23461 ctattactgt cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg
    23521 tgatccaaga aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc
    23581 tgggaatcca tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca
    23641 attggcgaag ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt
    23701 tcatctgcat tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac
    23761 agtattcata actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct
    23821 ccatcatacc gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag
    23881 tatcatttct gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt
    23941 ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg
    24001 taatgtaata gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact
    24061 gcttcgccta attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt
    24121 tgaaccactt ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca
    24181 tcttctgcta agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg
    24241 aacccagtca ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac
    24301 aatcctacaa caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa
    24361 gcgactttcc caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca
    24421 taataaaccg ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag
    24481 gcccgatttc tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa
    24541 acactttgcg gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc
    24601 ccatttttta ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga
    24661 cgctcgatct tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca
    24721 tcctctttct ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc
    24781 ttggcaaata aagctcttta tatgcttctt caaaaggttt cgttacaaat tcatcctcac
    24841 ggttttccaa taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg
    24901 caagttctgc atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac
    24961 caactaaagg gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt
    25021 tgtatttatc agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta
    25081 gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg
    25141 tttcatattc ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt
    25201 caaccgctga gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc
    25261 taccgcccca ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat
    25321 acgtatttgc tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg
    25381 ctttgccaaa tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt
    25441 ccgcatcagg acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag
    25501 ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc
    25561 cttggattgc tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga
    25621 gttcacctgg ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac
    25681 caagaccttt agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga
    25741 aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg
    25801 aaggtaaagt aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg
    25861 attaacctat cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat
    25921 ttgcttaaaa aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct
    25981 aattctttac ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag
    26041 ctaccatcga cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag
    26101 cgaccttcgc taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca
    26161 atcgttgatg gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt
    26221 tcttttaata tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct
    26281 tcatcagcta atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca
    26341 tctaacgctt tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag
    26401 tatgattttt attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt
    26461 gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg
    26521 gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt
    26581 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt
    26641 tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag
    26701 ccaaacaaaa atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg
    26761 attgtacacc gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc
    26821 aatttcaaaa aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg
    26881 caggagaata ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct
    26941 tatttccttt tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa
    27001 tgattttttc tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat
    27061 atatccataa cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca
    27121 cgacgtataa ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg
    27181 ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc
    27241 tatagctccg agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg
    27301 attcaaagca attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag
    27361 atacggattt ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg
    27421 atgagcactg actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc
    27481 tgctaagatt tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat
    27541 tcgttgcgta tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt
    27601 tctttgctgc tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag
    27661 ctgatagctg atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt
    27721 agctaaagca cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg
    27781 gggatggttt tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat
    27841 ctcattgaaa gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc
    27901 gaaacgaacg taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa
    27961 atataacggc atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca
    28021 ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc
    28081 atgaggaaaa attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc
    28141 aaataaacgg atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata
    28201 ataatacttg ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca
    28261 ttgatctgtc catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga
    28321 taatcctttt tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc
    28381 ccataaaaat ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt
    28441 gtttgattat ttttcttatg actagtaatg atatttttga atctactact atttgtaaca
    28501 ataaccattg tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc
    28561 aataaatcgc ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca
    28621 tttaaattca ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact
    28681 accccaattg catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag
    28741 atttttccgt ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt
    28801 ggatcgttta cttcagataa atcaattatt tcacctgctg caggagtact taacactata
    28861 tcctttgagg attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag
    28921 gaactgatca tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa
    28981 atcgggaaag acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca
    29041 gctactccac caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta
    29101 agagtcacac cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat
    29161 aaagataaac tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct
    29221 gcttgcgcag ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa
    29281 agattgttgg tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc
    29341 caaaatccgc cgataacagc accagataaa acagggctaa agttataaac agctaaatat
    29401 ccagctgcaa tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt
    29461 gggaccatca cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag
    29521 cttttgcgaa tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata
    29581 accgtacttg tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta
    29641 tcactatatg cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg
    29701 aaacgatctg tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag
    29761 aaaaatgcat cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa
    29821 ttcaaactcg tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct
    29881 aaagtcgggg caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc
    29941 tcagttcctc tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg
    30001 tcatacactt catttactgc atttcctact acgacttgaa acattcctcc agcttcgact
    30061 acagtgatca caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt
    30121 agggtaaagc gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg
    30181 acaaatcgga caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta
    30241 attaatgccg tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc
    30301 attcaattag taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact
    30361 tagccgagca aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca
    30421 aaggagacaa aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc
    30481 tgatactctg tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg
    30541 gcatttaacg gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct
    30601 atctttttat ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt
    30661 ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat
    30721 tcaaactcat ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct
    30781 tgctcagtag tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga
    30841 tacactggtt ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt
    30901 tttagtgtat tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt
    30961 cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg
    31021 ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa
    31081 cgggagatga tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt
    31141 ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga
    31201 agatctaaaa aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg
    31261 tttttttcat cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt
    31321 ttttcttgat tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct
    31381 accatcggaa taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga
    31441 atggtcaaaa cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta
    31501 tatgatataa gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt
    31561 gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat
    31621 aagaaaattc tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact
    31681 tccagttcta atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat
    31741 acggctgttt ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt
    31801 tgaaacaata agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta
    31861 tgtttcgtca cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa
    31921 taacctgaac gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt
    31981 ttgatttcag tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt
    32041 gttatccaat gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag
    32101 tccatttgct tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa
    32161 tagtcacttg cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc
    32221 attagactgg ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct
    32281 tccgctccca tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc
    32341 tgttcctgcc gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc
    32401 atcttttatt gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg
    32461 atcgtggatt cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat
    32521 gaataatggt aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg
    32581 atcaattggc caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc
    32641 atttgctccg ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata
    32701 ataactaaat gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa
    32761 cattactggt gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc
    32821 tcggcacatg acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac
    32881 attctctgga tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc
    32941 catcttagca tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa
    33001 catatgatga caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt
    33061 atctctagca gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt
    33121 gatctcatta aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg
    33181 cgcataaaat tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac
    33241 caatgctaag ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt
    33301 taatgtattg attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc
    33361 tcctcttgga aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc
    33421 cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag
    33481 ataatggagt gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat
    33541 actaggatgc tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc
    33601 ttccaaccat gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc
    33661 tggaaatatt tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag
    33721 tatagctcga ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa
    33781 ttgaatgcta ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga
    33841 aagaaaacac tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt
    33901 cataaggtcg taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa
    33961 taacccttaa agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa
    34021 taacgagcca ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct
    34081 tttccactaa ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca
    34141 atttcttgta catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt
    34201 ctttataatt tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac
    34261 gggcgttcca ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca
    34321 agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa
    34381 tatcttttgc ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt
    34441 gatagatata aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt
    34501 cttttcgata cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat
    34561 tccaaaataa actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt
    34621 ttttcagttc agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat
    34681 ctaatcctat gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat
    34741 aaagtttagc gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt
    34801 caccaaaagt tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg
    34861 tgatttcttt atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa
    34921 tacctttatc gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt
    34981 tccaattcaa atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt
    35041 catccaactg ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat
    35101 tgaccggatc acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct
    35161 caatgaacca cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga
    35221 tatttctttt ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg
    35281 ccatgattgc ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata
    35341 ctggagaaag ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa
    35401 tcccccgcaa atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt
    35461 gataaacaac tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg
    35521 tttctatttt ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc
    35581 aaatagctat tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat
    35641 tccctcaact gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact
    35701 tctttataaa cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata
    35761 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
    35821 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
    35881 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
    35941 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
    36001 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
    36061 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
    36121 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
    36181 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
    36241 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
    36301 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
    36361 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
    36421 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
    36481 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
    36541 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
    36601 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
    36661 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
    36721 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
    36781 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
    36841 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
    36901 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
    36961 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
    37021 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
    37081 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
    37141 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
    37201 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt
    37261 aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc
    37321 actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa
    37381 aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt
    37441 gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag
    37501 ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt
    37561 gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct
    37621 ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga
    37681 aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc
    37741 gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc
    37801 tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat
    37861 gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc
    37921 aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg
    37981 tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac
    38041 tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata
    38101 aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaacggca
    38161 atgaatgccg ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt
    38221 tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc
    38281 gattgcctgt gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc
    38341 tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca cgccagttga gacagcgaca
    38401 gatcgctcct tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc
    38461 tgcacctaaa ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc
    38521 gatcttttcg atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata
    38581 gatatctaca agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa
    38641 agcttcaata gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca
    38701 ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt
    38761 tgctgcctca ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc
    38821 gataccattt acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt
    38881 atccactatt acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat
    38941 agatagtctg tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat
    39001 ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg
    39061 gctgtttttt gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata
    39121 ccttgatgtt taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc
    39181 atttcctcag attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg
    39241 atactcacta ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt
    39301 gcataagctt gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta
    39361 tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt
    39421 cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg
    39481 acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt
    39541 tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga
    39601 atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta
    39661 ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg
    39721 gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga
    39781 ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat
    39841 tcgatattgc taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca
    39901 gccatttcat cccaccttta ttttgtttgt aagactagtt cacctctttt tgttgcaaat
    39961 gcaactattt tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt
    40021 tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta
    40081 actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc
    40141 ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag
    40201 caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa
    40261 aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac
    40321 gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt
    40381 tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat
    40441 ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag
    40501 tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct
    40561 ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt
    40621 tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat
    40681 gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata
    40741 aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa
    40801 taaatggcgt tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac
    40861 gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc
    40921 ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat
    40981 gagaagctgt ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac
    41041 ctctcggtaa acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt
    41101 attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata
    41161 gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc
    41221 gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa
    41281 tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc
    41341 acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa tcgtgatcac
    41401 ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg
    41461 atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata
    41521 gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat
    41581 ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg
    41641 ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat
    41701 gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca
    41761 gtccaatctt atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc
    41821 gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga
    41881 ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat
    41941 cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga
    42001 tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac
    42061 cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg
    42121 gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg
    42181 ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg
    42241 tcttcaggat attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc
    42301 cgctgtcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg
    42361 catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga
    42421 tatatttagc ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg
    42481 cgcctagcat agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat
    42541 tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc
    42601 ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc
    42661 attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc
    42721 ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc
    42781 aaactctttt ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt
    42841 ccaacttgtt cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt
    42901 ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct
    42961 tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt
    43021 ttccctactt tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt
    43081 gctgcggcta ctgtatctac atcggttgct gtagcattgt tgaatcccat cgaattaaag
    43141 atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg
    43201 atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag
    43261 aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg
    43321 gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa
    43381 ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc
    43441 cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac
    43501 acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct
    43561 gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc
    43621 atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg
    43681 atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct
    43741 tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt
    43801 ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa
    43861 ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc
    43921 atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa
    43981 cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct
    44041 aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc
    44101 atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc
    44161 gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt
    44221 cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat
    44281 gcctgagtca aaatattttc gatgataaaa ttgataacgc ccatg
//
LOCUS       DACERL010000017        42444 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.017, whole genome shotgun sequence.
ACCESSION   DACERL010000017 DACERL010000000
VERSION     DACERL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42444
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.017"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            111..620
                     /locus_tag="IXF54_000928"
     CDS             111..620
                     /locus_tag="IXF54_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1404206.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKIAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(691..1230)
                     /locus_tag="IXF54_000929"
     CDS             complement(691..1230)
                     /locus_tag="IXF54_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1404207.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(1370..1981)
                     /gene="rpsD"
                     /locus_tag="IXF54_000930"
     CDS             complement(1370..1981)
                     /gene="rpsD"
                     /locus_tag="IXF54_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1404208.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            2297..3094
                     /locus_tag="IXF54_000931"
     CDS             2297..3094
                     /locus_tag="IXF54_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1404209.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAYGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            3122..3757
                     /locus_tag="IXF54_000932"
     CDS             3122..3757
                     /locus_tag="IXF54_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1404210.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLTLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            3777..4550
                     /locus_tag="IXF54_000933"
     CDS             3777..4550
                     /locus_tag="IXF54_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1404211.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHKDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            4958..5755
                     /locus_tag="IXF54_000934"
     CDS             4958..5755
                     /locus_tag="IXF54_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1404212.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            5733..6146
                     /locus_tag="IXF54_000935"
     CDS             5733..6146
                     /locus_tag="IXF54_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404213.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            6130..8775
                     /gene="mutS"
                     /locus_tag="IXF54_000936"
     CDS             6130..8775
                     /gene="mutS"
                     /locus_tag="IXF54_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1404214.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAADVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            8793..10901
                     /gene="mutL"
                     /locus_tag="IXF54_000937"
     CDS             8793..10901
                     /gene="mutL"
                     /locus_tag="IXF54_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1404215.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKFVEEDEKIYEEELALHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            10923..11483
                     /locus_tag="IXF54_000938"
     CDS             10923..11483
                     /locus_tag="IXF54_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1404216.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(11568..13556)
                     /gene="treP"
                     /locus_tag="IXF54_000939"
     CDS             complement(11568..13556)
                     /gene="treP"
                     /locus_tag="IXF54_000939"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1404217.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            13779..14492
                     /gene="treR"
                     /locus_tag="IXF54_000940"
     CDS             13779..14492
                     /gene="treR"
                     /locus_tag="IXF54_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1404218.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFIEFARRRKV"
     gene            14569..15015
                     /gene="msrB"
                     /locus_tag="IXF54_000941"
     CDS             14569..15015
                     /gene="msrB"
                     /locus_tag="IXF54_000941"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1404219.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            15185..15787
                     /gene="ruvA"
                     /locus_tag="IXF54_000942"
     CDS             15185..15787
                     /gene="ruvA"
                     /locus_tag="IXF54_000942"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1404220.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            15800..16801
                     /gene="ruvB"
                     /locus_tag="IXF54_000943"
     CDS             15800..16801
                     /gene="ruvB"
                     /locus_tag="IXF54_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1404221.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            16830..17408
                     /locus_tag="IXF54_000944"
     CDS             16830..17408
                     /locus_tag="IXF54_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1404222.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            17460..17942
                     /locus_tag="IXF54_000945"
     CDS             17460..17942
                     /locus_tag="IXF54_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1404223.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIGENKITNNL
                     "
     gene            18059..18433
                     /locus_tag="IXF54_000946"
     CDS             18059..18433
                     /locus_tag="IXF54_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404224.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(19169..20521)
                     /gene="brnQ"
                     /locus_tag="IXF54_000947"
     CDS             complement(19169..20521)
                     /gene="brnQ"
                     /locus_tag="IXF54_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1404225.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVDQAPIQPNYQDHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPILMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLHVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            20668..21258
                     /locus_tag="IXF54_000948"
     CDS             20668..21258
                     /locus_tag="IXF54_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1404226.1"
                     /translation="MTKSRLEAFTDGVVAIVLTVLVLEIKIPNPSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(21386..22735)
                     /locus_tag="IXF54_000949"
     CDS             complement(21386..22735)
                     /locus_tag="IXF54_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1404227.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(22903..23643)
                     /locus_tag="IXF54_000950"
     CDS             complement(22903..23643)
                     /locus_tag="IXF54_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404228.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(23656..25248)
                     /locus_tag="IXF54_000951"
     CDS             complement(23656..25248)
                     /locus_tag="IXF54_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1404229.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(25816..26112)
                     /locus_tag="IXF54_000952"
     CDS             complement(25816..26112)
                     /locus_tag="IXF54_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404230.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            26288..27013
                     /locus_tag="IXF54_000953"
     CDS             26288..27013
                     /locus_tag="IXF54_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1404231.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            27006..27848
                     /locus_tag="IXF54_000954"
     CDS             27006..27848
                     /locus_tag="IXF54_000954"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1404232.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEEPLEESLETLRQKIDTIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            27851..28372
                     /locus_tag="IXF54_000955"
     CDS             27851..28372
                     /locus_tag="IXF54_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1404233.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(28615..29178)
                     /locus_tag="IXF54_000956"
     CDS             complement(28615..29178)
                     /locus_tag="IXF54_000956"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1404234.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            29429..29698
                     /gene="rpsO"
                     /locus_tag="IXF54_000957"
     CDS             29429..29698
                     /gene="rpsO"
                     /locus_tag="IXF54_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1404235.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            29886..32012
                     /gene="pnp"
                     /locus_tag="IXF54_000958"
     CDS             29886..32012
                     /gene="pnp"
                     /locus_tag="IXF54_000958"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1404236.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            32522..32788
                     /locus_tag="IXF54_000959"
     CDS             32522..32788
                     /locus_tag="IXF54_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1404237.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            32932..34923
                     /locus_tag="IXF54_000960"
     CDS             32932..34923
                     /locus_tag="IXF54_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1404238.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            35277..36578
                     /locus_tag="IXF54_000961"
     CDS             35277..36578
                     /locus_tag="IXF54_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1404239.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALVAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(36708..38078)
                     /locus_tag="IXF54_000962"
     CDS             complement(36708..38078)
                     /locus_tag="IXF54_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1404240.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(38260..38583)
                     /locus_tag="IXF54_000963"
     CDS             complement(38260..38583)
                     /locus_tag="IXF54_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1404241.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(38910..39518)
                     /locus_tag="IXF54_000964"
     CDS             complement(38910..39518)
                     /locus_tag="IXF54_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1404242.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            39848..40564
                     /locus_tag="IXF54_000965"
     CDS             39848..40564
                     /locus_tag="IXF54_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1404243.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTSADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            40577..41272
                     /locus_tag="IXF54_000966"
     CDS             40577..41272
                     /locus_tag="IXF54_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1404244.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            41356..41985
                     /gene="udk"
                     /locus_tag="IXF54_000967"
     CDS             41356..41985
                     /gene="udk"
                     /locus_tag="IXF54_000967"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1404245.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        13803 a         7885 c         8860 g        11896 t
ORIGIN      
        1 cagggcagct ttttcactac cttctgcggc acttcagttg gaattttctt cccatccttt
       61 ggtcgcggtc attaccaaga taaaattatc ttgcaaagga gaaatagaac atggaaaaaa
      121 ataatacccg ttcagccaaa cgctggacaa cgacagatgt gactcggatg gcgctagtca
      181 ctggtttata tgtggcagtg acgctggttt tgtctgtctt tagctttgga gtgattcaga
      241 ttcgtctctc ggagatgttc aactatctgt ccttgtataa caaaagatac atcgtcgcgg
      301 taacagcggg agtagctttg gctaatattg cttctcctct tggactgata gatgttgtag
      361 taggaagcgt cagcacgctg cttgtattgc ttatcaacta taaaatcact agtcgcatca
      421 aaaacatgaa aatcaaaata gctgtgacag catgcgtatt tgctttttcc atgtttacag
      481 tagctggtca gctaacgatt ttatatcaac tacctttttt cttgaattgg tgggtgattg
      541 ctttaggaga attggcatca atgattgtcg gtgggataat tatttattgg atcggtaaaa
      601 aagtggattt gtcaaaataa agatctgttt aatttaaaat atggcagcat ggaagaagac
      661 tttgagtctt cttccatgct gcttttttga ttaatttgta tgaagaaatc gatttagccg
      721 ttttttcatc aagataccga tataagctgc tccagttgcc ttgattgctt ccggcagtaa
      781 aaacggcagt agtccactgg caaatgcagt tgtaaaagta agatcaccac ttattttcag
      841 ccagatcgtt ccgcagatca atgcagcaag agagcccatt agattggcaa agattgcata
      901 caagtaatga gcatccgttc tttcgatcat ccaaccagtt atccacgttt gtaagagaaa
      961 tccaatcaaa tatccgccag ttggcccaaa taaaacagct atacctccag tcatccctga
     1021 aaatacagga agaccaatca agccaagtaa aagataaact gcaacagata tcaatcctag
     1081 ttttcttccg agaaaagttg ctgttaatcc aatagcaaat gtttgccctg tcaaaggaac
     1141 caaaccaaaa ggaatggtca tctgagaaag gacagaaata atcgcggcaa attccgcagc
     1201 aagtatcaat tctttcaatg ttcggttcat aacgccctct tttcgttaac taaatatttt
     1261 tatttggtta acgaaattgt aaaacaaagt gattactttg taaatagaaa atataaaaaa
     1321 agaccagcta tccaatagat agcggtcttt ttcaagaaag cttttgaaat tataagcttt
     1381 ggttgtagta ttcaacgaca agagcttcgt cgatttctgg atatagttcg tcacgttctg
     1441 gtaaacgagt caatgaacct tccaattttt cagcgtcaaa gcttacgaat gctgggcgtc
     1501 caacagttgc ttctacagct tcttttactg ttacgatgtt ttgtgatttt tcacgaactg
     1561 agatcacttg acccacttct acgtggtatg aagggatatc aacgcgtttg ccatcaacta
     1621 aaatgtgacc atggttaact aattgacgtg cttgacgacg agtcgttgca agtcctaaac
     1681 ggtaaacaac attgtctaaa cgttgttcta gtaagatcat gaagttaaca ccatgtttac
     1741 cttctttgat tttgctagct tttacgaaca agttacggaa ttgacgttcg ttcattccgt
     1801 acatgtgacg aagtttttgt ttttcagtta attgcatgcc gtattctgat tgtttgccac
     1861 ggctgttagg tccatgttga cctggtgcgt aagggcgacg tgctaattct tttcctgtgc
     1921 ctgacaatga gatgcctaag cgacgagaaa ttttccatga tggtcctgta taacgagaca
     1981 ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa taatccgttg aaaaattcat
     2041 aatcatatag tttgttcttc aatcttcacc tttgcagccg cggttacaca atcgaacccg
     2101 agacggggca acaaactagg gatgagttat tatttttctg ctgcattatt ttacacaaac
     2161 tacagtatat gatagatcat ccatgaatgt caagataatt agtacataaa aacgctttac
     2221 aaacctaggc tgtctttata aaatgagagg gtatgtgcaa atggaaaaaa gcgaaggaag
     2281 tttaaggaga aaatcaatga atccggtatc accattattt gagaaaatcg atgcttcaat
     2341 tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt tacgctgttc gagcggttct
     2401 cgcctgtttg tttttgactt taggaacggc tgtggcattt gcgatcgcga tgaaggggga
     2461 agatatcgcg tatggtctag gaaagatttt atacgcattt atgttcagct ggggtctggt
     2521 catgattcta tacatgaacg ccgaattagg aacatcgaat atgctttata tgaccgtggg
     2581 tgtttttcgg cagagacttg ctttaagtaa ggcagccaag attctatttg cctgtatttt
     2641 tttcaatatg attggcggta tattatgtgg atttctaatt tctatgacta cgccatttca
     2701 agatttaacg ttggacaact ttatgttaga atcaatagca gtaaaactag caaaacctgc
     2761 agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt gtcaataccg ctgtactgat
     2821 cagcatgcgg atgaaagagg atgccggaaa agtgatcaca gtcgtattca tcattttcat
     2881 ttttgctttt ttagggtatg aacacgtgat agcgaatttt cctgccttta cgcttgctta
     2941 ctttgcttct cacggacaga tggatggaat gacagtcagc aatgtgttac ataatttgtt
     3001 ttttgctttg gcaggaaatt atattggtgg cggtttagta atgggactgg gatatgcttg
     3061 gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc gttcattaaa ggggaagtgc
     3121 catggaagtt ttagtttttc caaaaaatga ggaaaaagcg ttacaaatgg aaaagtacat
     3181 gaaaaatctt ttttcctttg ctggagtacc taagccggaa cgcgctcttt tacaacaacc
     3241 gttactggat gagagcaaaa atttatctat ttctgaagtt ttagcttttg ttcattatta
     3301 ttatgaacaa atcgaaagag agtatcagta tgtagcgatc gatttagtcc agaaaaacat
     3361 caaacggctt acatttgaag acttagtcgt attgttgaca ctagttcaga aaaaagaatg
     3421 gtgggacagc atcgactctt ggagaaaagt atacagcaca tggtgtaaag cctacccaga
     3481 gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa gatttttggc tacgtcgaat
     3541 agcaatcacg ttgcaattag gttataaaca gcaaacagac acaggactat tgatccgagc
     3601 aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa aaagcaattg gttgggcatt
     3661 acgcgattat agtaagaccg atgccgattg ggtaaaaaga cagctgacaa cagatttgag
     3721 taagttagct gttcgagagg caagtaagta tttataagaa agcgagacga caagagatga
     3781 gacgcaaatt atttgctttt gatattgatg gaacgttgtt agattcaaac aaacaagctt
     3841 tagatagcac gagagaagca ttggaaaaat tacgcaaagc cggtcatctg gtcacgattg
     3901 ctacagggcg gagccgattt catgcgcaag agatcattcg cgatcttgat tttacgaact
     3961 acattttatg taatggagcg gccggttttt tagaccatga acaagtatat aagaatttac
     4021 tagatcgcga ccagttggat gcgttcgttc aagaagcgca tcatgagcag atcgatacag
     4081 cttttgtcag cttagacaat atcaagcgtt cttcttccaa tgatgtaacg gtgatggaag
     4141 aagcgatgca ttctttcggt gctgtcttgc ctgaattaga tcagtttttt gcagaggaac
     4201 aagaaatata ccaagcgtta gcatttttcg atcaaacata tgatgggaag ttctcttcat
     4261 atgataaaat tcgatttgtc cgctggcatg agaacagtgt agatgtcgta cctaaaaatg
     4321 gttctaaagc agcgacaatt ctgcacttag cagaacaagt aggcatccct cataaagaca
     4381 tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt gcgcatgtca ggaattggga
     4441 tagctatggg gaatgctgtg ccagaagtac aagcagaagc taagatggtc actgatacaa
     4501 acgatcagga tggaatttgg aaggcattga aagagttaag cgtgatttaa aagaggttgt
     4561 gaaaaagctg ttctgcatca agtaataagc ccagccaaac aaaaatagct cctcctattt
     4621 ttcgtttttc tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
     4681 tcgtgacaga agtggtcagt ttcaagaaat aagaagggat ttacgaaaat tgcttctcaa
     4741 atttttgtaa aattacggct tatttctgaa gaagtagatg aaacaacgtt tattcgggaa
     4801 atgggactgt gacaagatgt ttgtcatagt cccatttttt tattttattt ttcaaaacta
     4861 agaaatacca ggaaagagtt ccttttgttt gaccttttct ttcttattga aaacaactat
     4921 aatgtagagt gatgttaaaa gagaagagga atttagtgtg cgggtattgt ttattggtga
     4981 tgtagtaggt tcaatgggac gagagatgat tactgaatat cttcctagat tgaagaagaa
     5041 gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc gcttctggac gtggaatcac
     5101 agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat gtcgtgacga tggggaatca
     5161 tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa gctaaaaaga tggttcgtcc
     5221 ggcaaatttt ccggaaggga cgcctggaca agggatggtc tttgtcaaag tgaaccaagt
     5281 cgaactggca gtgatcaaca tgcaggctcg ttcttttatg gtggacttgg atgatccttt
     5341 tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt acgccgatca tttttgtaga
     5401 ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc tggtttttag atgggaaagt
     5461 ttcagcagtg gtcggtacac atacacacgt tcagacaaat gatgcgcgaa ttttgccaag
     5521 aggaacagcg tatctgacag acgttgggat gacaggtccc tatgatggta ttttgggtat
     5581 gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta ccaaaacgtt ttgaagttgt
     5641 ggaacaaggc cgatcgattt taagcggctg tatcttggaa ttggatgata cgacaggtca
     5701 tgcaaaagaa atccagctga ttcagatcaa cgatgatcga ccgttcatgg agtagaaaga
     5761 gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga agtaaggaaa ctgacaaaac
     5821 ttttaagaga gaatgaaacg atcattcgct acaaagaact tgaagaaaag atccagcaaa
     5881 accagtattt agctgagttg agagaaaaaa tcaaacaagc gcaaaaagat gctgttcatt
     5941 ttgcccacta tgacaaacca gcagcagaac aagaagcaat caaacaagcg gatcaattca
     6001 tgcaagaatt tgatcagcat ccgcttgttg tagcttatag aaaacagctg ttggaagcag
     6061 atgatctgct tcatcattta acaactatga tacaagaaga aatcaatgga caaatagagg
     6121 aggaaaaaca tgcctcaaaa aactaaaaat actcctatga tggaacaata tctaagcatc
     6181 aaggcccaat accaagatgc ttttttgttc tatcgtttag gagactttta tgaactattt
     6241 tacgaagatg cgatcaaagc gtcacagctt ttggaattaa cgttgacgag cagaaatcga
     6301 aatgcagaag aacctattcc tatgtgcggt gtacctcacc atgcagcaca aggatatatc
     6361 gatgccttga ttgaaaaagg ctataaagta gcgatttgcg agcaggtaga agatccaaag
     6421 acgactaaag ggatggtcaa gcgcgaagtg atccagctag tcactcctgg tacagtcatg
     6481 gacagcaaag gattgtcggc aaaagacaac aattttttaa cagctgtagt tagtcaagga
     6541 aatgaatttg gctttgctta tgccgattta agtacgggag aattgaagac ggctcggttg
     6601 catgatgaag aagctgtttt aaatgaagcc tctgcattgc agacaaaaga actcgtatta
     6661 ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg ggcgtttagg gctagtcttt
     6721 tctcaacaaa atgagatgga agaaaacgcg gaatttagct ttttgacaag cgaactagtc
     6781 gatccgctgg aaaaagaagc aaccggcaaa ctattgactt atttatcggt gacacaaaag
     6841 cggagtttag ctcatatcca aaaagcagta gaataccaac cagatcattt tttgaaaatg
     6901 gattattatt caaaattcaa cctagaatta agccaatcta ttcggactgg gaaaaagcac
     6961 ggcactcttc tatggttgtt agatgaaaca aaaacagcaa tgggtggccg tttactaaaa
     7021 caatggctag accgtccgct tatccaggca aaacaaatta aagcaagaca agaaatggtc
     7081 gcttctttgc tagacagcta ttttgaacgt atcgatttgc agtcagcatt aacgaaagtc
     7141 tatgatctgg aacgcctagc aggtcgagta gctttcggta atgtcaatgg gcgagacttg
     7201 atccagctga aaacttcttt agaacaagtg ccaatgatca gagggttgat tgaaggaatc
     7261 gatcaaggtc aatggcaaag tctgctttca gaaatgcagc cgatggacca tctcgtccaa
     7321 ttgatcgacc aagctatcca agatgatccg ccgttacaaa taacggaagg aaacatcatc
     7381 aaagatggat tcaatgagca gctcgatatg taccgttctg ctatgagaaa cggaaagaaa
     7441 tggttggcag aacttgaagc aaaagagcgt caagaaacag gaatcaagaa cttaaaaatc
     7501 ggatttaacc gcgttttcgg ttattatata gaaattacaa aagcaaatct tggaaatgca
     7561 gaactggaga agtatgaacg aaaacaaact ttagcaaatg cagagcgttt catcacacca
     7621 gaactaaaag aattggagac acaaatcctc gaagcagaag aaaaatctgt tgatttagaa
     7681 tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg ctatccagcc gctacaagta
     7741 cttgcaaaag caatcagtgc agcagatgtg ctacaaagtt ttgcaacgat cagcgaacgc
     7801 tatcagtatg ttcggccaga acttgtcagt gacaagcatc agcttctgat caaagacgga
     7861 cgacatcctg tagtagaaaa agtattagga catcaggaat atatccctaa cagcgtagaa
     7921 atggcagaag acgaaatgat cttgctgatt acaggaccga atatgtctgg taaaagtacg
     7981 tatatgcgac agttggcatt aactgttctg atggcgcaaa tgggctgttt cgttccggca
     8041 aaacaagcag ttttacctat ttttgatcgg atttttaccc gaatcggtgc aagtgacgac
     8101 ttaattgctg gacaaagtac ttttatggta gagatgatgg aagccaatca agccctgcgt
     8161 tatgctacgc caaacagttt gattttgttt gacgaactag ggcgaggaac agcaacctat
     8221 gacggaatgg cgttggccca agcaatcatc gagtatatcc atcgtcatgt acaagcaaaa
     8281 acactctttt ctacccatta tcatgaactg acggtacttg agaaagaact tccgcagctt
     8341 aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag ttgtcttctt gcataaaatg
     8401 atggatggac ctgcggataa aagctacggc atacatgtgg caaagatcgc aggattgcct
     8461 tccaatctac tagaacgagc agcagatatc ctgcatacgc tagaaaatgg cgataacgga
     8521 caccatactg ttccagctga agtagaagag aaaactaaaa cagccaaaca ggagcaaata
     8581 aaaccacctg cagaaaaagt aaatcaagac catatacaag accgtgtaaa agaagaaaac
     8641 caacagcttt ctttattcag tgaattatcc gaaaatgaaa cagaagtcat tgaatcactg
     8701 aagcaaataa atttactaga aatgacgcca atggatgcac taaacacatt atatgaatta
     8761 caaaaacaac tgtaggaaga aggcggatat cgatggccaa aatccaagaa ttatctgaac
     8821 gtttagctaa tcagatcgct gcgggagaag tagtggagcg accagcttcc gttgtgaaag
     8881 aattagtgga aaatgcaatt gacgccggca gtactcagat tgatatctta gttgaagaag
     8941 cgggactgcg gacgatccaa gtgattgata acggagaagg cattttggca gacgacgtag
     9001 agaatgcctt caagaggcat gcgacaagta agatccataa tcgcgacgat cttttccgca
     9061 tacgaacgct tggatttcgc ggtgaagctt tgccaagtat tgcctcggtt tctgaaatga
     9121 ctctggaaac ggcaaatcag gaagaaaaac aaggcaccta tctgtcgctg aaaggcggag
     9181 aagttattga gcatcgacca gcaccactgc gtcaaggaac gaagattacc gtcagcaatc
     9241 tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac actgcacaca gaattagcga
     9301 acatcgggga catcgtcaat cgactggcat tgagtcatcc atcgattgcc tttcgtttag
     9361 tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg cgatctgaaa caaacaattg
     9421 cgggaatcta cggattatct accgcaaaaa aaatgctgaa aatcgaagga aaagatctag
     9481 attttgagtt atttggctat gtttcattgc ctgaggtgac tcgtgccagt aggaattact
     9541 tatcgacgat catcaatgga cgttttatca agaacttttc cttgaataaa gcaatcgttg
     9601 caggttatgg gtcaaaatta atggtcggtc gtttccctat tgctgtattg gaaatcaaaa
     9661 tggacccgct tttagtagat gtaaatgttc atccgacgaa gcaggaagtc cggctgtcga
     9721 aggaaaaaga actaacagag ctaattagta acgccattca aaaagcattg tctcaagaaa
     9781 atctgattcc aagtgcagcg gataatctac ggtttaagaa aaaagtaccc gatcagccta
     9841 aaacagaaca aatcgaaatc gatttgtcct acgcttttga agaagaaaaa cctaaaaagc
     9901 caagtagttt gaattttgat cgagagacag gaaccttttt cgtagaagaa ccaaagaaca
     9961 catttgttga cagaattgtt gataagtctg tggaaaatgt tgaaaactat tctggttttt
    10021 ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac ggtggataag tttggtgaaa
    10081 atgttgataa ctttgtagaa aaaacagtgg aaaaatctga aaagaaagaa tttcccattg
    10141 tggataacat ggagaaaaca aatgaagaac aagcagataa attcgtagaa gaggatgaaa
    10201 agatttacga ggaagaattg gcgctccatc ctgaatttga tttcactcaa ggaaacgaca
    10261 aaacattaga acaagccatc aagaaattag aaacggaaaa accagtacaa cgttttcctc
    10321 agttggaata ttttggacag atgcacggaa cgtatctttt tgcccaaagt agcgatgggt
    10381 tgtacatcat tgatcagcat gctgcccaag agcgcattaa gtatgaatat ttccgggaaa
    10441 aaattgggga agtatctgat gatctgcaag aattgcttgt tccatttgtg ttagactatc
    10501 caaacagtga tgctttgaaa ttaaaagagc aaaaacactt gctagaagat gcagggattt
    10561 atttagaaga atttggtcaa aacagcttta ttgtacgggc ccatcctact tggtatccca
    10621 atggagaaga agaatcgatt attcgggaga tgatcgatat gctgctgaca actggaagtg
    10681 tgagtgtaaa aaaattccgt gaagcaacag caatcatgat gagttgcaaa cgatcgatta
    10741 aagccaacca ttatttgaat gaagcacaag cacgtgtgct gctgaaggat ctggcacgat
    10801 gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt gattcacttt accaattcgg
    10861 atatggaaag gatgttcaaa cgaatccaag acccgcatta agtcaaagag aagagggaaa
    10921 acatgaagat tgtattagca tcccaatcac caagaagaaa agagttattg gctcatatcg
    10981 tttcagaatt cgagattgaa cctgctgata tcgatgaaac gccatttcca gacgaacagc
    11041 ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg aactgtctgg gaacaagatg
    11101 aaaaagaaga ccatttagtg attgccagtg acacgactgt tgttctcaat caagagatca
    11161 tgggtaaacc agaagatctg gctgaggctg aattcatgct gaaaaaacta agcggggaaa
    11221 cacatactgt atatacagct gttgttttaa aaacaaagga acgagaagaa cggatactag
    11281 cagaagcaca tgtcactttt tatcctttga ccgacgaaga gatcaaacgc tacttggaga
    11341 cgggggatta tgcggataag gcaggggcat acggcatcca aaatgctgcg agtgtattcg
    11401 tcaaagaaat ttctggagat tattacagta tcgtaggatt tccgattggt gctgtcaatc
    11461 aggcactgaa aacattcatt taaacaaaaa agcggactat ctgattttcg attgaaaatc
    11521 catgatagcc cgtttttttt attgaaaaga ttacttatct actctgacta agattttact
    11581 tcagctttac cgactgcttc gcccgggcga acaagtcctg attggtcaag agaaagtcct
    11641 gcaacttttt cattattggt caaagcaaca ataatgtcag ttttcttgcc agcttgttct
    11701 atttctggca aatccatttc agcaatcagg gtctctggtg tgacaaggta tccttctttc
    11761 acgaatacat tgaaaggaac accctttagt tcaacagtat ctaatcccat gtgaacaagt
    11821 acttccagcc cctcattaga aagaatacca atggcatgtt ttgtttcaaa gacactcgtc
    11881 actttaccgt ttactggtgc atagactttg ccgtttgacg gttcgacagc ataaccgtca
    11941 cccatcattt tttgtgagaa aactgggtca tttacttcag ttatttcctt gatggtccct
    12001 gcagcgactg cgaataatgt ttcttttgtt gcaactgccg taccagttgc tgaaacaggt
    12061 tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt gaagttgtac atcagcagca
    12121 ttttcaggaa cagcaggatc aatcttattc aaaattccct gacgtctaaa gatgatcgtc
    12181 aagccaaatg gaatgatgac agcaatgatc attgcgatga tgaaaggtac ccatgtttct
    12241 gcttggatag ctagtattcc tggcaaaccg cctactccga tcgcgttagc tcgaacaccc
    12301 atcaagttag caaacatgcc agctaatcca gagccgacca ttgcagcgac gaaaggatag
    12361 acgtatttca aattgattcc gaacatagca ggttcagtta cgcctaagta acaagaaatc
    12421 attgctggaa tcgagatttg ttcttctttt ttattcccac gatgtaagaa gatgatggct
    12481 aagactgcag agccttgggc gatatttgat aaagcaatca tcggccataa attcgttgat
    12541 ccgaaatctg caatcagctg catatcgatg gcattggtca tatggtggag accagtaatc
    12601 actaaaggag catacaggaa tccgaatacg gcactaaaca accagctgat agaagacgtc
    12661 aatccagtat ttacgatatt tgagatccag cttccgactg tccagccgat aggacctaaa
    12721 ataacgtgtg cagctaaaac agttggtaac aatgaaaaca atggaacaaa aatcatcgag
    12781 atcgactgag ggatatattt gcggaagaag atttctagat acgctaacat aaaccctgcc
    12841 agcatggctg gaataacttg cgcttgataa ccaatcatct ggacttgagc aaaaccaaaa
    12901 tcccaaaatg gaatatctgc tgcggctgtg gaagctacac tataagcatt taataattgc
    12961 ggagaaacta acgtaattcc taaaacgatt cctaatattt gtgtagtacc catctttttt
    13021 gcaatactcc aagtgatacc tactggaaga aaatggaaaa ttgcttcacc cggtaaccac
    13081 aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa cgattgttcc accaagactt
    13141 tcaaactgga taccttcaag tacatttctg aatccaagga tcaatccacc gacaatgatt
    13201 gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa tagcacgttg aacgggatga
    13261 aggttttgtt ttgcagctgc ttttccttgt tctttggaaa ccccttcaac tccagatact
    13321 gcaacgaagt cgttataaaa agtagaaaca tcgtttccta tgatgacttg gaactgtcct
    13381 gcattcgtga acatcccttt tacgctaggt atatcttcga tcgctttttc gtccgctttt
    13441 cctggatcat tcaggacaaa acgcatacga gtcgcacaat gactaactgc agcaatattt
    13501 tcttttccgc cgatatcatg taacagcttc tcggcatctg cttgatattt tgccatatca
    13561 ataaacccct ttctcttaac ttgtctatgc aagttgtttc ttcatttact atactttttt
    13621 tcggagagaa ttgcaagcgt tttattaaaa agtacgagag aaaatgatat cgttttaaaa
    13681 agcttaaaat aagcttttct taaaaatagg aacattgaaa tacaaaaaga gctatagtat
    13741 agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat gaataagttc aatgaaattt
    13801 ttttagattt ggaacagaaa attttagcga aagaatatcc tccgcattcg cttttaccca
    13861 gtgagaacca attgatcaaa atttatggtg tttcacgaga aacaattaga aaagcgctga
    13921 atcttttgac tacggaaggt tatattcaaa aaaaacaagg aaaaggttcg atcgtactgg
    13981 atcggaatcg cttcgacttt cctatttctg gattgactag ctataaagag ctacaggaga
    14041 cccaacgtat tccaagtgaa acgatcatcc atacgttaga agaaacagaa gtgacaaaag
    14101 cattgaacga gatcactggc tgggagatcg gtgcgcctgt ttggcatttg atacgcgaaa
    14161 gaaaaatcaa tggcgaagta gttatattag acaccgatta tctattaaag gaaatcgttc
    14221 ctattttaac tccagcacaa gcacaaggat cgatctatga atattttgaa aatgagcttt
    14281 ctttgacgat tgcttacgcg caaaaggaga tcacagtaga agaagtaacg gaaatcatga
    14341 aaacagcgat ggatcttcac gatgacactt atgcagtagt agtcagaagc ctcgttcatc
    14401 tggaagatac ccgctgtttc gagtattctg agtcggttca ccgattagat aaattccgtt
    14461 tcattgagtt tgcccgacgc cgaaaagtgt aaatagttca aaagaaagca aattgttggc
    14521 atctgtccac tttggcgcta tgataggatc acaaaggggg cttttttaat ggataaacca
    14581 ataagaccag aaaaagaaga actgaaagaa aagttatcac ccatggctta tgcagttaca
    14641 caagaaaatg caacagaacg accatttagc gggaaatatg atgattttta cgaaaagggg
    14701 atctatgtag atatcgtcag cggtgaaccg ttattcagct ctgcagaaaa atatgatgca
    14761 ggttgtggct ggccatcatt cagcaaacca ataacgaaaa gacaagtgcg cgaaaaagcc
    14821 gatttttctc atggcatgca tcgaatagaa gttcgaagca aagaagcaga ttctcatctc
    14881 ggacatgtgt ttaccgatgg accagtagat caaggtggtc tacgttactg catcaattct
    14941 gcctcactga aattcattcc atatgaaaag atggatgaat taggttatgg ggaattcaaa
    15001 tcacttgtag aataatggaa atacacaaag agcggtacaa tacggggatt tactcgatcg
    15061 tccgctcttt ttttattcaa gcgattgcta tttgaaagaa acgctcccca gactcctttt
    15121 ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt gtgcaaaaga aagaggacta
    15181 gaatatgtat gaatatctga caggtgtggt tacctttatc aacccctatt acatagtagt
    15241 agaaaccaat ggtatcggct accagatcgc actaggaaat ccttaccgct acagtagcaa
    15301 actaaatcaa aaaataaaaa tttatgtcca tcaggtgata cgtgaagatg cgcatcttct
    15361 atatggattt gattcgctag aagaaaagca actgtttttg cgattagtaa gtgtttcagg
    15421 aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa gaccactccg gtttgattca
    15481 agcagtagaa tccagtgatg tcacttattt gacgaaattt ccaggagtag ggaaaaagac
    15541 agcacaacaa atgatcttgg atctaaaagg aaaattcggt gagctgagta tcgatacacc
    15601 ttttagtcta tttgatgaag caaacacaaa agacgctaca gctctatctg aagcaatgga
    15661 agctctctcg gctcttggtt acagtgatcg agaaatcaaa cgtgtagaaa aacaattaaa
    15721 agaagtcgat aatcagacaa cggatgagta tctacgccaa gcactcaagc taatgatgaa
    15781 aaaataagga ggagaccaaa tgacagatga atacttgtta tcaccagaat ctggcgaaaa
    15841 tgaactgagt ttggaaaaat ctcttcgacc acagctgctt tctcaatata tcggacagag
    15901 aaaagtcaaa caagaacttc agatttacat cgaagctgcc agaaatcggg aagaaccttt
    15961 agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa acgacgatgg ccatggtcat
    16021 tgctaatgag atgaccgtaa atattcgtac gacgagtggc cctgctatcg aacgtccggg
    16081 tgacttagta gcaatcttga atgaactgga agcaggggat gtcctgttta tcgatgagat
    16141 ccatcgatta cctagagtgg tagaagagat gctttattca gctatggaag atttctatgt
    16201 agatatcatg gtaggacaag ggccaaccgc acatccggtt catttccctc ttcctccctt
    16261 tactttgatt ggcgcgacga cacgggcagg tatgctttct gcacctttac gagatcgatt
    16321 tggcatcatt tctcatatgg aatattatga cgaacaggat ctgcaggaga tcgttttacg
    16381 ttcttcggat atttttcaaa cagagatcat tgaagaaggg gcattggaaa ttgctcgccg
    16441 atcacgagga acgcctagaa ttgctaatcg cttgctcaaa cgggttcgtg attttgcgca
    16501 agtccaagga gacgggaaaa tcgatcgtcc tatagcggat caggcactta ctttgctaca
    16561 ggtcgatcag gctggattgg attatgttga ccagaaattg ttgcgaacaa tgatcgagtt
    16621 atatggcggc ggaccagtag ggttaagtac gctttcagta aacataggag aagagcgtga
    16681 gacggtagaa gatatgtatg aaccgtatct gatccaaaaa ggatttttaa agcgtacacc
    16741 tagagggcga attgctactg cttatgccta tgaacatttt ggttacgact atcatatatg
    16801 atttttgctt ttagattgga ggaaaatgaa tgagtgatac aacacgtacg aaacaattga
    16861 tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt tcgaaaaatc agcgtccaaa
    16921 aaatcgcaaa tgaagcaaaa atcaatcgac aaacatttta ttatcatttt acagataaat
    16981 acgatttgct tcgttgggtc tattgtgagg atgcattgca ttatttagat ccacaatgtc
    17041 tttcacttga taattgggaa gagcaagcat tgaaaatgct gaaagcaatt gctgagaaga
    17101 aagaatttta tgcaaatact gtgctttctg acccagaaat tttacaaaaa gagttttctc
    17161 aactgatcca aaaatcattt atcaagattt ttgaacaaat ggatgaagaa agacaacttt
    17221 ctgcaagaga caaactattt tatgctcgtt ttttttctta cggttgcagt ggtgtattag
    17281 tcaattggat cacgaatgaa tatgaagaaa caccgatgga aatcgctatg cagcttttcc
    17341 gtttagccaa ggatacggaa ttatattctt atcgtcttta tgcaaaagaa gagttggaag
    17401 aacagtaata gaaaaatttt ttgttggaaa agcaatgatc gctaaaagga gatggggaaa
    17461 tgacaaaaat tttatttgtt tgtttgggaa atatttgccg ttcaccgatg gcagaaggct
    17521 tgctgagaaa acgaatagca ttagaaggaa gggaagcaga attttttgtg gattctgctg
    17581 ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg aacgaaaaaa atattaaacc
    17641 aagaaaaagt agatatgacg aatatggttg ctcggcaaat cacaccccac gattttgaaa
    17701 cttttgattg gatcattggc atggatcaag aaaatgtaga agaactgaaa cgaagagcac
    17761 cacgctcagc gcaggggaaa atccatttgt ttttaagtag cattcctgga aaagaaaaag
    17821 aaaatgttcc cgacccatac tataccaata actttgatga gacatatcat ttgatcacag
    17881 aaggcctgaa tcattggtgg aatatttgga ttggcgaaaa taaaataaca aacaatttgt
    17941 aaagttgacg ctttcttttt aaggcgatgt tagagcattt ccttgttttt ttgtaaaatg
    18001 ttaggtaaaa taaaattaat taagtaacac tttcttagct aaaggaggag tgcattcgat
    18061 gaacgaagag acagtatcgc caatgaagaa gcttacgaag ttagttactc atacttcgta
    18121 tcaacttttt gattcttttg taaatgacac tgaagaacat gctaagaatg tacgattgca
    18181 aggaaagttt attcttgagc tgcaaaaagc tgctactgaa catagtctag ttatcttaca
    18241 agtaagagca aatcgtttga gtagcaagta tgaaacgatc tcaggctggg tggcgacaaa
    18301 aacggtcgaa aaagaccgag tgatgatccg cctacaaaac gaagagcagc agatccggat
    18361 ggttcctgta gaacaaattg aaaaaataac aatcctttca ccagcagggg aacaggaacg
    18421 attatcaaaa taatgtatac cagatagaaa agcacgtatg agcttgtacc atcaatcatt
    18481 actaatcaaa aataaccgaa tgctttaagc tgatatcagt aaaacggaac gttttaggat
    18541 gagtatcaca gcatgacagc attcggttga ttagttgaat cgattggatg gtacaagttt
    18601 ttttgataaa aaaaggtcct gctagccggg gagctagcag gataaggagt taaaaatgaa
    18661 aaagtgttgt tagggttgtt tgttggtatg cttatataat agtcattagt tgtgaatatt
    18721 ttgtgacgaa agttttcgaa agttgttaag tttttttgcg aaaactattt ttatatcata
    18781 tatatgtcta aaatctatat tataaaagaa atgttgtttg atcagcttct cttcggtatt
    18841 agttcaaaac aaaacggcaa agtatatgga gctactttgc cgttttgttt ctctagtact
    18901 caggtcagtt tatagacgct caataaacgg tgttcaaaag ccagtttctc ggtattaagt
    18961 caaatttatg caaaaatgtg agaagccatt ttcacaaaat atttcttaat acttgaaact
    19021 aagcggcttt ttcacgacct ctcaataaac ggtgttcaaa agtcagtttc tcggtattaa
    19081 gtcaaattta tgcaaaaatg tgagaagcca tttttcaaaa ttacttttta ttgctcaagg
    19141 ctgaacgtct ctttcacaac ctcttttatc aatctgctac ttcttctttc ttagcgaaac
    19201 tccaaatcag tccgataaca aaaccgataa ctgctggtac gatccacccc atccccaatt
    19261 tgaaaaacgg caagtagctt tccgcaacat gtaaaagatt ttgaacaata ggtgtttgtt
    19321 tgatagtttc tgggcaagcg ttcaacccat caaaaatcga agcaatcaat gtaaaataag
    19381 tagtcattcg ataaacagcc gtacgctgct taaacaaagg accaactaac actagtaaaa
    19441 tcagtgtcat tgctaatgga tagatgaaca tcaaaatagg caacgaaaat tgaatgatat
    19501 tcgtcaagcc tacgtttgcc gctaaacaag gaaggatact agcaagtgca acaaagaatg
    19561 cgtaatttct ttgcggaaac aaatctgtga atgtttctgc aaaagctgta atcaaaccaa
    19621 taccggtctt caaacaagct aatataacga taaatgctaa taagatactg ccataagtac
    19681 ctaaataatg atcagcaatc tgagccagcg tgatcccgcc attttcactt agagcaaagc
    19741 tacctaagct catagcacct aaataagcga gcaaagtata aatgattccc atcaatataa
    19801 tgctgatcgc accagatttg atcgtgtctt tagcgatttc agcaggtttt gttacaccca
    19861 tgttttggat cgtcgtaacg atgatgatcc caaatgctaa agcagcaagc gcatctaatg
    19921 tgttatatcc ttgagtaaat cctgtgaaaa aagcatggtc ttggtaattt ggctgaatag
    19981 gcgcttgatc gacagagcct aatggattca aaaaagcaag gagaagcaaa gcacctaata
    20041 aaaccaaaaa tgcaggattc aggaactttc caacataatc taatatcttt gtcggttttc
    20101 ttgaaaacca ccaagccagc acaaaaaata caatactgaa aattgctaga tatagcgtct
    20161 gtctgctagg atcgataaat ggtgctaaac caatctcgaa agatgtggtt gcaaggcgag
    20221 gtaaagcaaa aaacggtccg ataactaagt aaagcaagat ggtaaaaacc atcgcatatg
    20281 tttgatttat ccgttttgct aattcatata cacctgagct tttcgataca ccaatggcaa
    20341 tcacccctaa gaagggaaga ccgataccgg taaccaagaa acctagattc gcccaaaagg
    20401 tagcagcgcc tgcttcttga cccaaatgaa cgggaaagat aagatttcca gcaccaaaaa
    20461 atagaccgaa aagcatggag cctataaaaa gatagtcttt aaatgataat ttttttgtca
    20521 tacataactc ctttagggtt tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt
    20581 ttcagaaaat ttcctatata aaacgatggt tatttaataa cgtaagtgat aaaataaaag
    20641 cgctgaatca tctgaggagg aatcatcgtg acaaagtcga gattagaagc atttactgat
    20701 ggagtggtag ccattgtttt gacagttctt gtattagaga tcaagatccc taatccatct
    20761 agctttcatt ctttatgggt catccgaaat acattattag cttataccat cagctttata
    20821 tttgttgcgg tgatctgggt gaaccatcat cgtatatttc agatggcaga acgaattaat
    20881 taccgagtgg tctggtcgaa tatctttttg ctcttttggc tgactttatg tccttctgtt
    20941 actagttggg tcgggcgtaa ccctgaagct ttttggcctg agatggctta tgtcttagta
    21001 tacacgatgt ggagtttttc ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa
    21061 ccagattccc atgtcgtgag agttttgtca aaggaccgtc gaagtcttat ctccatgatg
    21121 attaacttat gcttgattgc cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt
    21181 ttttttgtat ctggtatttg gattttttcc tataaaaaag cagatgccta ttatcagcgg
    21241 ttgtttccgg ggaaatagaa aagaggctga gacagaagct tttaactcca agaaataagc
    21301 cgaaatttca caaaaattta agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct
    21361 gaccacttct gtcacaacta ctttcttact tcttacgttc atatttcata tgatagatta
    21421 gtaatagaag caagaaccac acaggtgtta gtaataaagc gattctcgta tcttttccta
    21481 ggaacaagac gacacacaca aaggccatgt aaagaaatac cagataatta ctaaatgggt
    21541 acaacggcat tttaaatgaa tgagcttttt tgccgaggat acttttacga tatttcatgt
    21601 gcgtgtagac aagcatactc cagataaaca agaaacaagt agtggcaata ctgcttacta
    21661 gtgtgaatac ttcactaggc atcacgtaat tcaagataac agaaatgaaa atgacgatag
    21721 tagaaaatgt caaagcattc cctggcacat gatgtgttgt taactttttg aattttttag
    21781 gggcactgcc ttcttgcgct aaagaacgaa gcatacgtcc tgtactatat attgcactgt
    21841 tgcaggcaga agctgctgca cttaatacga ctaaattgat caaggaagca gcaatcgtga
    21901 tcccaatttc cgaaaatact tctacaaatg gactgttatc tggattcaag ctgttccatg
    21961 gatagattgc cataatgaca aaaagagatc ctaagtaaaa taagatgatc ctgataggaa
    22021 tattattgat agcttccggc aacacttttt caggattttc tgtttctcca gcgactaatc
    22081 caactaattc tatcccagca aaagcaaagg ttaccatttg gaaagctaag ataaatcctt
    22141 ttgcaccagt tggaaagaat ccgccatgag accatagatt cgtgatactg gctgtgccaa
    22201 tatctgtcgt atagtgggtg aggatcatat aagctcctac gaggataaag gcaatgatcg
    22261 ctacaacttt gatcaatgca aaccaaaact ctaactcacc aaacaatgag acagccacca
    22321 tattcaagca aagaagaaga cctaatgcaa tcagttcagg aagccactga ggaacaccag
    22381 gcgcccagta gcgtacatac attccgatgg ctgttaaatc ggccattgca atcgataccc
    22441 agcagaacca atacgtccag ccagtgatga aagccgcttt tttgccaaga tattctgcaa
    22501 caaaatccag aaaagaatgg ttattggtgt ttgataacag aagttcgcct aacgcacgca
    22561 taatgaaaaa acagatgcat ccagtaataa gataggcgag caatatcgaa gggccagcta
    22621 gctggataga ctttccggaa cctaaaaaca accccgttcc aatcgctccg ccaatagaaa
    22681 tcagttggac atgtctattt ttcagtccac gctgcagttc ttgctcttca ttcattttta
    22741 aatcatccta actaaaaaag tttaattact tccatattaa gagagttttc tgaaaaacac
    22801 aactaagatt ccaaaatttt cagaaattta ccttttatga gtaattcata aaaaagaact
    22861 gcggtaaaca ccgcagctct ttgtcccaat cagctattta tcctaagcat ttaaaatacg
    22921 tgaaaggaat tcttttgtac gttcttcttt cggatggaca aagatatctt ctggtgtacc
    22981 ttcttctgca atcacacctt tatccataaa aatgacacgg tcagatacat cttttgcaaa
    23041 ttccatctca tgcgtcacga taagcattgt caagccagta tgtgctaagt ctttgatcgt
    23101 attaagcact tcgccaacca tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa
    23161 tacatctgga ttcattgata atgcccgagc aattgcgaca cgctgtttct gtccaccaga
    23221 taattgtgct ggtttggctt cgatataacg atccatacca actttttcca gattttcgat
    23281 tgctactttt ttggcagttt ctttatctcg tttcaggaca gttgtttgac cagacataca
    23341 gttttccaag acattcatat tgttgaacag gttgaaggat tggaaaacca tacctagatt
    23401 cgttcgatat ttaggtaaat tatatttagg agctaataca ttttctccat tataaatgat
    23461 ctcgccgcct gttggttttt ccagtaaatt gatacagcgc aagaaagtag atttaccaga
    23521 accggaagaa ccgatgatcg tcacaacttc acctttattg actgttacgc tgatatcttt
    23581 caatacttca ttttcaccga agctttttct tagatgtttg atttcaataa ttttttcatt
    23641 catgattctt cctccttatt cctgcaaatt tgaattgtcg atttcttcaa gcttggtata
    23701 agctgaagga ccatcgattt tcttttctac cgcccgcagg attctggtga tcgtaaaggt
    23761 catgactaag tagatgatcc cgacgatcgt aaatgtttgg aagaattgga aattcgcacc
    23821 ggaagcagag tttccttgga agaacaaatc agctacgccg attacactta aaactgctgt
    23881 atctttgata ttgataacaa actcatttcc tgttgctggc aagatattcc gtaaaacttg
    23941 tggaataaca acttttctca ttgtttggcc atgggtcata ccaatggctt gcgcagcttc
    24001 gaactgccct tcatctactg caaaaatccc cccacgaacg atctctgtca tgtaagctcc
    24061 agtattgaga gagacaataa acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc
    24121 gagtgccaga ccgtagaaga tgaccattgc ttgtaccatc atcggtgttc cgcggaatac
    24181 ttcgacatag atagaaagaa gtgcatttcc gattttttgg aagaagcgag tcagtgcatt
    24241 tcctgattca ggtaccgaac ggaagacacc aatcagtaat ccaagtgtag taccgacaac
    24301 tgtcccgatc aatgcgatga acaaggtaag tccagcgcct cgtaagaaca tattgccgta
    24361 ttgtttccaa atcgtagtga aatcagaaat gatgccaggt ttttcttcac tatcagttgc
    24421 agcaggctga tctttgactg cttggtccat aattttggtt cgttcatcgt tagagatgcc
    24481 agaaagaatc tggtttactt tttggatatc cgggtcgcct ttacgcatac caacagaaat
    24541 ctgaacatct tccgggtttg tctggaagcc atctgcaccg ctgaattcaa gcatttccaa
    24601 gtctttgttc acactagttg ccgttacacc ttccggacgt tcgctgacat aaccatcgat
    24661 cattccagaa gctaaagctg ttctcattgc tgagaaatta tccattgctt gttgtttctg
    24721 cacatctggt atctgatcga tcacagtata gtggaatgta tttaactgag cagtgatctt
    24781 tgctccagaa agatctgcta aactttttgc ttttgcatat ttgctatctt tttgaactac
    24841 tactactaat tgtgattcgt aataaggatc ggtaaaatca atttcttttc gtcgttcagc
    24901 agtagggctc attccggcga tgatcgcgtc gatcttgcct gattgaaggg caggagccag
    24961 accatcccat ttcgtttgga cgatgactaa ttttttccct aaaccatctg ctactttttt
    25021 agcgatttga acatcataac cgccggcgta actattttgg ccttgaattg gcaaggcatt
    25081 attctcatct gtttgttgag accagttgaa gggtgcatac ccagcctcca tacctacgcg
    25141 aaactcatcg ctcggtgttt tttcatctgc atttgccaga gatcccacac tgaatagtga
    25201 gaagacaaaa gcaaatagta cggcaactga aaccatattt tttttcattt ttttctccct
    25261 ttctgattat atgatacacg agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa
    25321 ataaaaagcg accgatttat agatataaaa aggccgctaa aattccataa ggtgataaac
    25381 ctctcacaaa acatagcgca acttagcatc actgctaaga cagtccgcaa gctcttaact
    25441 tgcgtcccaa catacactcc aagcaaaaaa gtgcacattt cggcgatcct ccctattcct
    25501 gcttctccat gtttgcttca ctccacaggg ttaatgattc tcgcgacctc tacctcattg
    25561 attgaggcat ctcgtattca gttatttata caaaaaaagt ttacgatact cgagaggctt
    25621 tgtcaatcta taactgctgt tttttgaaaa aatactgata aatatagaaa tctttacttt
    25681 taccgaaaaa acaatcaaaa tgtgaatcat ctatccattg caaacgatag tataattata
    25741 cacaaaaatg tatgtaatac aataaaaaaa gaactagaac agaaaatttc ctgtgtccag
    25801 ttccatctcg atcatttaag ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa
    25861 aagaacaacg acgccataaa tggctaatat tagataaatc aaacttgagt attgggtgat
    25921 tccccagccc catgttcgat atacggctaa tgtaatggct aaaacaattg cggcaatcac
    25981 gttgaataaa gcaaaagccc aaagatttcc attttctttg cgtacaagat aaaaaacaga
    26041 gaaataaacg cttaacgcta accaggcgat acaagccaga ataacgcccc aataaataaa
    26101 gaatggtaac atatcgattc acctcacttt tatacagcag tattgtaaca cgttttcatt
    26161 cttttgtcag tgaacattgt gataaattgc tcaaatgccc ataacacttt tttattccca
    26221 tttttctgac ttttcaacaa taaatttgtt cgagtatggg ctataatgga aagtgaggtg
    26281 acaaaacatg aaacaattaa cagtagacga agcagcaaaa gagatattag cttatcagca
    26341 aattacattt ttgaccggtg caggggtatc cacaccttct ggagtgccag actatcgatc
    26401 tttgtcagga atctaccacg ggatggagca gccggaatac ttgctgagtc atcaagcaat
    26461 ggtgaatgaa ccggataaat tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa
    26521 acccaatatc attcatttgg aaatagcaca tctagcgaaa gaaaaaaatg tttgggttgt
    26581 ttcccaaaac atcgacggtt tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca
    26641 tgggagtctg tatcaatgtc attgtcgaaa atgtcaacaa acggtagact ggaaagaata
    26701 tcttcacagc gacaaacatc aagcatgcgg aggacaaata cgcccagaca tcgtgttata
    26761 tggtgaagga tttcaagatg gagtcatgga gcaggcggct tatgccgtca gccaagcaga
    26821 gctgatcgtc attgttggaa cgagttttca agttcatccg ttttgtgatc tggtacagtt
    26881 tcgccagccg tcagccaaaa tcttagtaat caatcaaacg cctgtttatt tagcggagcc
    26941 ttatacgttt attcaagaag acggagcggt tgttttcaaa aaaatacaag aattaggagc
    27001 agattatgac tgaattcaaa accgaaaaag aaaaaatgat tgcaggcgag ctttattttg
    27061 ccaatgatcc tgagctggtt gccgaccgta tgtttgcaag aagtcaaagc caaatcatca
    27121 accaagcaga atcagcggaa ctgcgatccc aattgttgaa agagactttt ggccgtacag
    27181 ggaaaaaaat ctatatggag ccagttatca attttgatta cggatataat atatttgttg
    27241 gggagaattt ttatgcaaat ttcaattgta catttttaga tgtcagcacg attgaaatcg
    27301 gtgataactg catgttcgct ccgaatgtcc agctttacac ggcgacgcat ccactacatc
    27361 cagtgaaacg taacagcggt ctggagtacg caaaacccat taaaatcggt aataatgttt
    27421 ggttaggcgg aggagttatc gtcacgccgg gtgtcactct aggggacaac gtagtagtcg
    27481 gcgcaggttc agttgtgacc aaatcttttc ccgacaatgt agtgattgca ggaaatcctg
    27541 ctcgtatcat caagacagta gaggaagaac cgttagaaga gtctcttgaa acattacgtc
    27601 aaaagatcga tacgatcgat cgagagttgg ttgctttatt agaaaaaaga atggatacgg
    27661 taactaaaat aggacaagtg aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag
    27721 agcagcaagt attggataaa gttgttagtc tgctgaaaaa taagggatat aaggaaacaa
    27781 taacagacac gtatgttgat ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg
    27841 aggaataagt gtgatcgaag gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa
    27901 tacagaaaaa ccagtgagtg ctagcaagtt tgcgaaaact tttggagtca gccgtcagat
    27961 cattgtaggc gatattgctt tactacgagc tgcaggtaaa agtatcgtgg caacagcaag
    28021 aggttatatc ctcgaaacag aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca
    28081 gcatggaaaa gatcaaacag aagaagaact gagactaatc gttgaaaatg gtggtgaaat
    28141 cattgatgta attgtggaac atccccttta cggtgagtta gcaggaactt tgcacatcaa
    28201 aacgccacaa gatatcgatt cctttatgag acgttatcaa aaaagtaatg ctactttgtt
    28261 atccgaatta acaggcggca tccatctgca tacgatccgc tacccagaaa agcatatgct
    28321 gaaaaacatc aaacaaaaac tagctgaagc aggaatcctc tacgaaggat aacatcgtag
    28381 ctgctacttt ttatcctaga aactattcta tcatctctga gataaacacc catatggaaa
    28441 aaataagaaa agatgcgtat ccaatacaca gaattttttg gaattgcgat ttctagatgt
    28501 ctatctaaat ttcgattagt ggagaaacat aaaaaaggga agtgcagctt ttgagcactc
    28561 ccctttttta taaagctttc ttgtagcata gtatcgaagg aacctgccaa ttctctactc
    28621 gattaccaat acgccttctt ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa
    28681 catcacaccg ttgatatggt cgatttcatg ttgaacgacg atcgattcat aattcttcaa
    28741 acgaatcttg tgtttttcac cattcatatc ataataagac acagtgattt tggcatggcg
    28801 aacaacatat cctggtactt ctcgatcaac agataaacag ccttcacctt cccctaaaca
    28861 agcatcttga acagaatggc tcaaaatttt aggattatac ataacagtac ttagagaagg
    28921 tccatctgcc tctgggtcag gacttggtac gtgaacagca atgattcttt tagaaatatc
    28981 taattgagga gcagctaagc caacaccgcc tcgtaagtgt agttcttcag ctttgattgg
    29041 atcttggctg ttttttaaga actcaagcat ctcttttcct aaagagatat cttcttcgct
    29101 taatggaaga gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat
    29161 atcatccatt gtaatcatgg aatatgtacc cctttcttaa aagactgtgt ttcaagtcta
    29221 ttttcgcatt atagtttacc acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta
    29281 ccttttctta atgattaact acttgatttt caacccggtt tcaggtaaag tatagctgtg
    29341 tgctctaggc acatgacctg ccacttggtt aagcgaatca ccaacgtttt actcagtcgc
    29401 aggggaataa tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca agaatgaaat
    29461 catcaacgaa tacgctcgcc atgaagggga tactggttct ccagaagtac aaatcgctgt
    29521 attaactgaa gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa aagaccatca
    29581 ttcataccgc ggcctaatga aaaaagttgg tcaccgccgt aacttgttag cttacttacg
    29641 taaaactgac gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt
    29701 ttagtcaaaa agagtgggat tcacatgaat ctcacttttt ttactcgtat atcaattaag
    29761 taaaattccg tgatcaagct agatggactc ctactaacca aatgaaagct gctcaaaaga
    29821 cggctttcgg cagttttcat ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag
    29881 aagttatgtc agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg
    29941 aagtcggtca gctagcaaaa caagcaaacg gagccgtttt agtccgttat ggagatacag
    30001 tagtcttaag tgcagctgtg gcctctaaag aagcaaaaga tacagacttt ttcccattaa
    30061 cgatcaatta tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt ttcatcaaac
    30121 gagaaggccg tccaagtacg gaagcaacat tgactgcccg tttgatcgac cgtccaattc
    30181 gcccgatgtt tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg
    30241 tagaaacaga ctgctcgcca gctatggcag caatgctagg ttcttcttta gcactatcaa
    30301 tctcagatat cccatttgac ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg
    30361 agtacgtctt gaatccaaca gtagaacaag cagaacaaac agatatcgaa ttgactgttg
    30421 ccggaacgaa acaagccatc aatatggttg aaagcggcgc gaaagaagtt tcagaagaag
    30481 acatgcttgg tgcattgctg tttggttttg acgctatcaa agagttagtt gctttccaag
    30541 aagaaatcgt tcaagcagta gggaaagaaa aaatggaagt gactttactg caagttgatg
    30601 aagtattgaa aaaagaaatc tttgatgctt cttacgctac aatgaaagca gcagtaatga
    30661 ctgaagaaaa acttgctcgc gaagataata tcgagcaagt gaagatcgat atccgcgaag
    30721 cttatgctga gaaatttgca ggtcatgaag atgaagatca cttgttgaaa gaagttaaac
    30781 aaatcacaga agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc gataagatcc
    30841 gtcctgacgg gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc agcttattgc
    30901 cacgtgtaca tggatcaggt ttgttcacac gaggacaaac acaagcttta tcagcttgta
    30961 cactcgcacc tctaggagag caccaaatca ttgatggttt aggggtggaa gtcagcaaac
    31021 gattcatcca ccactacaat ttccctcaat tttctgttgg ttctacagga cgtgcaggct
    31081 ctccagggcg tcgggaaatc ggacatggcg cattaggtga acgtgcgtta gcacaagtaa
    31141 tcccttcaga agaagaattt ccttatacga tccgtttagt agcagaagta ttggaatcaa
    31201 atggttcttc ttcccaagca agtatctgtg ctggcacatt agcattgatg gatgcaggtg
    31261 taccaatcaa agcgccagtc gccggtattg cgatgggact tgtttctgat ggtgaaaact
    31321 atacgatcct aactgatatc caagggctgg aagatcatct tggcgatatg gactttaaag
    31381 ttgccggtac aaaagatggt atcactgctt tacagatgga tatcaagatt caaggaatca
    31441 cagaacaaat cctaactgaa gcattaactc aagcaaaaca agcacgtatg gaaatcttag
    31501 aagaattgac ttcaacgatt gctgctccac gtgaagaatt aagccaatat gcaccaaaaa
    31561 tcgaaatgat ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata
    31621 cgatcaacgg aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag
    31681 taagtattgc ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt
    31741 tgaccaaaga agtcaaagtt ggtgaagttt atctagggaa agttgtccgt atcgaaaaat
    31801 tcggtgcatt cgtcaacttg atcaaaggaa aagatggact tgtccatatt tcccaattag
    31861 caaacgatcg tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag atccttgtga
    31921 aagtaactga aatcgataaa caaggacgcg taaatctgtc aagaaaagca atgttgaacg
    31981 aagatggttc aacgaaaaat gaagaaaaat aatttaattc ataggaataa tgtacttgtg
    32041 atcgtgaagg tgacaggggg acaataatta ttcgaaaaaa ggggactgtg acaaaagccg
    32101 tgtcacagtc ctttttttgc gaataaacgg tgttcaaaag cttgcatctg cggtaataag
    32161 tccagccaag ttaaaaacat taggagctgt ttttacttgg ctgttcttat tacttgatgc
    32221 agaacagctt ttttacaacc tctccaataa acgatgttca atcagctgtc cctcgatatt
    32281 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttatttctct aatactcagg
    32341 tcaaatcgct gatttcacaa cctctgattc acggtgttcc agaagcagct tctacggaaa
    32401 taagctgaac acttttgtta caacttcttt ttttctggag tttcttggcg catatttttg
    32461 gagtccttct gaataaagcg taaaataaaa gtgagaaata tggataaagg agggaaagga
    32521 gatgctgaat tttttatggt cgttatttat cggcggtata ttaggtttga tagctggcgc
    32581 aattttagga aaggaagtcc cgggaggcgt atctggaaat atcattatcg gttttttagg
    32641 tagttggctc ggcgaatttt tactaggacc actgggaccg gaactcggcg gtttttattt
    32701 gataccagca ttattgggtg caattttttg ccttgctctt tattcatttg tggcaagacg
    32761 tatcagagaa cgtccaaaga aagaataatt ttattcaaat aagttgacac ttcagtgtgt
    32821 tacttggata attagaagca atttaggaaa gcgggtgtca tggataagaa tatcatctat
    32881 cattcaagct tacctaaagg atatccatat aacaagaaat gagggaaaaa cgtggcaaca
    32941 acgcacaata ccaaaaagat atcaatggct ggcctgcttg tagcgatggg agtcgtctac
    33001 ggagacatcg ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga taatggcgga
    33061 ttgtcaggaa tcaatcctga ttttgttatc ggatcagtct cattgatttt ttggacattg
    33121 actctactga caacaatcaa atatgtcgtg attgcattga atgcagataa ccatggtgaa
    33181 ggtggaattt tctcactcta cacacttgtc cgaaaaggtg ggaaatattt gatcattcct
    33241 gcaatgatcg gaggagctgc cttactagca gatggggtat tgacccctgc tgtgacggta
    33301 acgactgcga ttgaaggctt gcggggaatt ccgcagtttt ttgaccggtt cggtaataac
    33361 cagaacgtga tcgtcattat cactttagtt attatttttg ttttattcat ggtccaacgc
    33421 tttggtacag atattgtcgg aaaagcattc ggaccaatca tgttcgcttg gtttactttt
    33481 ttaggcgtaa tgggattaat gaacttcagt caagactgga cagttatccg tgcactgaat
    33541 ccttattatg ctatccagct gctttttagt gcagataaca aattaggctt gttcattctt
    33601 ggaaatattt tcctggctac gacaggtgca gaggcgcttt attccgatct tggacatgtc
    33661 ggcaaacgaa atattcgtct gagctggcct tacgtcaaaa tttgtttggt tatcaattat
    33721 tttgggcagg ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag
    33781 aacctgaatc cattcttcca aatgatgcct caaagttgga tcgtgatcgg cgttgctttt
    33841 gcaacagtcg ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt tacgctagtt
    33901 tcagaagcaa tcaagttgaa actgctgcct cggttaaaga tcatgtatcc tgggaacagt
    33961 atcgggcaaa tgtatatccc agcagtcaat ctgattttat ggatcgcctg ttctctagtg
    34021 gttgtcacat tccgaacgtc tcatcatatg gaagctgctt acggtctatc gatcactgtg
    34081 acgatgttga tgacaacagc actgctttac ttttatctta tccaaaatgg gcattctcgt
    34141 tggcttgcat atttagttac ctttttcttc ggtgcgatag aagtcgtttt ctttatctca
    34201 agtgttgtca aattcttccg aggcggtttt gtagccgttc tgattggttt gatcatctta
    34261 gtcgtgatgt ttatttggga acaagggaac attattcgcg aatcgtcaac cgaggatgtg
    34321 tctttgaggg attatatccc gcagcttaga gaattacgtg atgatacttc tttaccaatc
    34381 tatcagacaa atgtggtctt cttggttcct gatatggctg aagataaagt tggccgacag
    34441 ttcatctatt cgattttaga taaacggcct aaaagagcac gagtctattg gtttgttcat
    34501 gtggaagtga cggatgagcc atatacgaaa gaatatcaag tagatatgat ggacactgat
    34561 tttattgtcc aagtgaatct gttcttaggt ttcagggtcc aacaggaaat caacgtatat
    34621 gttcgccaag tcatccatga tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga
    34681 tactctctga caccagggcg agaagttggc gatttccaat ttgtcctgat tgatgaagta
    34741 gtttccaatg tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca
    34801 atcaaaaaaa tcgctacctc gccagaaaca tggtttgggc tagagtacag cgaagtaaaa
    34861 catgaaactg ttccgcttat cattggaaat actcggaaaa catggttgaa agagcggttg
    34921 tgaaagtagc gctttgacct gaactaatgt cgaatgtcag ctacttgaac accgtttatc
    34981 agaggtcatg aaaaagctgt actgcaacaa gcagtaagaa cgagcagata aaaacagctt
    35041 ctcatgtttt tcatctgttt gggcttaatg ccgcagatgc aagcttttga ataccgtttt
    35101 tatcagaggt catgaaaaag ctgtttagca acaagtaacg caatgatttt gaagtgataa
    35161 ggagatgtgg ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga atacgcatta
    35221 ttttcaatct ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg gagaatatgg
    35281 ttatcaattt cattatttta tttttgatga ttgcaattac ttcatttttt gtagcaagtg
    35341 aatttgcttt agtgaaaatc agacgatcaa gattggaaca actagaaaag gaaaatgtaa
    35401 agaacgccaa gctagctctt catgttacac accatttaga taactattta tcagctagtc
    35461 aattaggaat tacgctaacc ggtttgatca tcgggtgggt cggtgaagga tctgtcgcgg
    35521 ctttactaga accagtgatc ggtaaactac cttttagttc ggctattagc agtacaatct
    35581 ctgtggcgct gggatttatt ttagttacgt atatcgatgt ggttattggc gaactgcttc
    35641 ctaagagcta tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt aaaccacttc
    35701 attattttta taaagtaatg tttcctttta tttgggtatt gaatcattca gctgcagggc
    35761 tagggaagct attaggtgtg cgtctcgtat ctgaaggaga agagacattg tctcaagaag
    35821 agctgacatt agtcgcattg aattcttatg aaaaaggaga gttgaccaaa gaagaatatc
    35881 attatttgga aaacgtgttt gaatttgatg ataccttggc aaaagatata caagtagacc
    35941 gtacgtccat gcaagtattc gaagcttcag aaaccgtcaa agaagcgata aaagcttcga
    36001 ttgaacaagg acatactcgc tatccggtta tcttagaatc aaaagacaat gttttaggtt
    36061 atgtcacctt gccagatttg atcaagcaat cttataaaga tgaccagctg acagtagaac
    36121 agttgattga agagccgatc gtaacaactg aaacgatccc tataaaaaaa ttattgacga
    36181 tcatgcgaaa aaagggaaag catattgcta tcttaaaaga tgaatacgga gggacaagcg
    36241 gcttagtgac gattgaagat attttagaag aaatcgtcgg cgaaatccga gatgagacag
    36301 atttggacga agcattggta gcggaacagt cagatggctc ttatatcatc tccggcaaac
    36361 taacattaga tgatttccag cgttattttc atgtagagat tccagagttt gaagaaacga
    36421 attttactac acttgcgggc tttgcttcta gccgatataa agaaataaaa gcaggaacaa
    36481 tcattgagat tgcatcattc cgtttcacgg tattagaata ccagcatgca catatcgatt
    36541 atttcaaagt agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc tgtgacattt
    36601 tttgtcacag cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag aagatatttt
    36661 caataaaagt tttcattttt agccgcaacg atttctttta atcaaggcta tcttgcttct
    36721 gcatgttcta atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt catcgaacgc
    36781 atataagagt aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga agctgctgct
    36841 gctcctgggt atccccacat tcctgtaaga acgatagctg caaaagaacg cataagcaat
    36901 tccatcatac cggcgatcgt agggatcttg ctttgtccaa gcccttgaag tgtgtagcgt
    36961 aaaatgaaca ggactgctaa gatccagtaa agagacccat tgatattgaa gtaggtttgt
    37021 gctaattcaa atacacgtgt ttctgatggg ctaacaaaca attgaacaaa gaaatgaccg
    37081 aagaaaatga cgatagcacc tgctaagaga ctaaatccga cactcatcag taaagtttga
    37141 cgtacccctt gcaagattcg gccatattct ttcgcaccat agttttgtgc agtgaaagtt
    37201 gccattgcaa taccaaatga catcatcggc tgatttgcaa attggtcgat acgaccagca
    37261 gcggcttgag cagctacgac atctgtccct agactattta gtgctgcttg taacacgata
    37321 gcaccaacag cgatgatcga agattgaaac gccattggca aagcagcatt taggtgtaca
    37381 gcaaactctt ctttttcaaa tttgaaatct tttttgcgta actgtaacaa aggcattttt
    37441 ttcttgatat acactagaca cagtacgctg gaagaaactt gtgcgattac tgtagcaata
    37501 gctgcacctt ccacacccat gccaaaatag ataataaaga tcaaatcaag tacgacgttg
    37561 atgatgaccg caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag tgcgcgaatg
    37621 acattagaaa gcaaattgaa actgactgat gcaaagatcc ctaataaaat aattgaaata
    37681 aatgtttgtg cttgatcaag tatttcagga ggagtttgca tcaattgaag catcggacga
    37741 ataaacagga gacttaggac agttaaaatc acagtgacga caagactaat gacaacacta
    37801 gcagcaaaac ttttcttcag accccgataa tcttttgcac cgtaacgttg tgcggtaata
    37861 atggctaaac cagctgttaa accttgggca aagccaatga tcaaaaatgt caggcttcca
    37921 gttgcaccaa ccgctgccag cgcattttta cctagcgtct gacccacgat gatcatatcg
    37981 atcatattgt agaactgctg aaaaacattt ccgatcagta aaggaacagt gaacatcaaa
    38041 atcagtttag cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc ttatatcttt
    38101 aaaatttagt tagtagtata tagcgtttat cctgagaata ccaattagaa atcaatgata
    38161 atttatgaaa ctccataaaa agaagccaag aattgagtca aagaatcaac aacttggcct
    38221 ggtctgtaaa ataagggaca tccacgttta tctatcacct taatattgtc tgatctcact
    38281 tggtaaaagg atcgtttctc tcgtaccgtc atcataggcg tatacttgag caggaaattg
    38341 gttcgaatag gcaaacggca aatcaatatc catttccaca ttgcttcctg cttgagaaaa
    38401 atgcatcgtc acttttccac gattttcgat caaatcaaag gttaatatat tttctaatgg
    38461 aatgactccc ttaagatcta ggtcaataat aaaccatata ctgtcaatca attcatctgg
    38521 caaacttgat acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa aactttcaaa
    38581 catctgttta acctccttcg aatcaacgaa tgttttattt ttataattaa tcctaggata
    38641 aactagaaat acttacttca acgagggttc attctaataa agaatcagga aaaatgcaac
    38701 aataattgaa tacttttttt caaagtataa atttttataa aggtacgata aaagtatgcg
    38761 aggaaaagct tggtaaatta ggatttttgc gtttgcaaag ctatttgtaa aagtggaagc
    38821 ctcatatgaa aataaacgat tagaaagaaa aaaaggcgtg ttaaaagctg ctgacccgcc
    38881 gcttctaaca ccacctgatt gtttcagtat tattcttctg ttgaaccgat tacttctggt
    38941 tcgccacctt cacctgtgct ttcttcttct tcactttgag cgactacagc agcgatttgc
    39001 tcttcactgt cggtcacgaa tttgtaatcg tcgttttttg gaagatcggc tactgtaata
    39061 gaatcaccta attccaagtt tgtcacgtca acttcaattc gttcaggcaa tttgtctggt
    39121 gtagcagata cgactactgt atacaagttt tgagctaaca ctccaccaga tttagcaccc
    39181 actgattcgc caacaagaac gacttctgtt tcgacttctg tttcttccga catgtttaca
    39241 gatagaaatt ctgcatgttc gaaatgattg gtaaaagtgt tcgtttggat cttgtgaacc
    39301 aatgtattga ctttttgtcc atcgatgtcc atcgtgataa ctgtgttcgc tccatgttca
    39361 cgcaacaatt tgcttagtgt caaaccatcg aatgagatag gggtactttc tactttatac
    39421 ccataaacaa cagctggaac tttcccttcg tgacgaagtt tgtttcttaa tgaacgggga
    39481 cgtacttctc ttttacttac ttctaatgat actgacataa ccaaaattcc tccttaaaat
    39541 gtgactgtct gctttggtaa cggacagatt ttatttaagt gaattctaaa ggctaatctc
    39601 gtgcacatca tcgaaggcag ggcgcttaaa aaagcgcaaa aggagagatg cgcgcatctc
    39661 cccttagaaa gtcaggattc aacttttctc caccaggtca ttacgctgtg tatgagaaat
    39721 ccaattcact ttactattta accgtaacgt gaactgttaa ttaagtcaag gaataactat
    39781 gatgatgtat ttgcaagcaa gaatattcaa ggtataatta attagtagta ataagaaagg
    39841 aaacagcatg cgtttagata aattaataga agaaaaatta gaaacgacac gcaaggaaat
    39901 gaagcggctg tttgctttga agcaagtgat gatagatggc acagtcgaat ttcgacaaaa
    39961 tcgaaatgta gatagtttac tgcatcaaat cgaagtagca ggtgaacact tagaaacaaa
    40021 tgagtcctat tatcttctca ataaacctga aaaagctgtt acagctgtat cagatccaga
    40081 aaagacgaca gtaatcgatc tgattgcaag tgccgatcgt ttccaaccat tatatccagt
    40141 gggaagacta gatagagata caacaggact attgctgatc acttcgaatg gacaattggg
    40201 atatgaaatg ctacttccag aaaaaaaggt ttcaaagact tatcaagtag ttgtgaacga
    40261 aagggtgaca tcagcagacg taacagcatt tgaaaatgga atcgtattcg ctgatgggta
    40321 tcaatgtaag ccggctgatt taaaaatact caaagcagac aagcaccaaa gtacggtttt
    40381 attgacgatc caagaaggaa aattccatca agtcaaaaag atgttcttag cccaaggaaa
    40441 gaaagtgacc gcattgaaac ggctaagcat gggaccattg gaattagatg aacagctagg
    40501 agtaggcgag tatcgatcgt tgacattagc agagatgaaa aagttaagta tttattttaa
    40561 ataggaggtc aaaaagatga aatataaaac tttattgttc gatgtagatg atacattgtt
    40621 agattttcaa ttaactgaag ccaatgcttt aagtgctttg tttgcagaac aaggaatcac
    40681 attgacacca gaaatcgaaa ccagctataa ggctttgaat catcatttat ggcaggagtt
    40741 tgaaaaaggc agactatcac gagaagaaat aataggcagt cgttttgggt tacttttcca
    40801 gcaatttggg aaaacagtag atagtgatca aatggaaaaa cgctaccgcc actacttgaa
    40861 ccaaggtcat gatcttgttc caggaagtct agaaatcctg aaaaaggtcc atccgcatgc
    40921 tgaactttat attgtgacga acggtgtgtc gcacacgcaa catcggcgat taacagatgc
    40981 aggaatgctt gattacttca aggatatctt tgtctcagaa gctgtcggag cgcaaaagcc
    41041 gatgaaagaa ttttttgatc atgtcttttc ccacattcct aattttgata aaaagcaaac
    41101 tgtgatcatt ggtgattctt tgacttctga tatcaaagga ggaaatatgg cagggatcga
    41161 tacgatttgg ttcaatagaa ataaattacc agaaatcgaa gaaatccagc ctacttatcg
    41221 aatcgattca ttagaggaac tttattcatt attggaaatt gaaaaggatt aattttcaga
    41281 aaaatatagc attttcgtgt tttttcgcta taatagatta gaatgaaaaa acggatacga
    41341 aagaggtttt agtcaatgac aaaaagcaag ccaattatca ttggtgtgac gggtggatcc
    41401 ggaagcggaa agacaagtgt cagccgtgcg attttcaaca acttcccgga ccattccatc
    41461 atgatgcttg aacaagattc ttattacaag gatcagagcc atttgagttt tgaagaacga
    41521 ttgaacacaa attacgatca cccatttgcc tttgataatg atctgctgat tcagcatgta
    41581 ggcgacttac tgaactataa agccattgaa aaacccgtgt atgactatgt agcgcataca
    41641 agaagtcagg caacgatcat tcaggaacca aaagaagtga tcattctaga aggaatcctg
    41701 attttagaag acgaacgtct gcgtgacttg atggatatca aagtatacgt agatacagat
    41761 gatgacattc gtatcattcg tcgtatcaaa cgggatatgg aagaacgggg acgcacattg
    41821 gactccgtga tcgaacaata tttgacagtt gttaaaccga tgtatcatca atttatcgaa
    41881 ccaacgaagc gctatgcgga tatcattgtt ccagaaggcg gagaaaacca cgttgctatt
    41941 gatttgatta caacaaaagt agctagtttc ttaaaccata aatagtgcgc agcgaaacag
    42001 ttgaacggac tgcgaccgtt tgaaaagcaa ggattctgct ttttgaacag tcaccagcca
    42061 actgtttttt tatttataac aagaggttgt gaaaacagca tcttgaccta agtattaaaa
    42121 agaagaaacg gaaaatagct gtttatattt taccgttttt tgagctaata ccgaaggaca
    42181 gctgattgag caccgtgaag tagaggttgt aaaaaagctg tctttgcatc aagcgataag
    42241 agcatccaaa taaaacagct cctcacgttt ttcgttgggt tgggcttatt atcgtagatg
    42301 caagcttttg aacaccgttt aacgagaggt tgtaacagaa gtggtcagat ccaataaata
    42361 agaagaaact tacgaaaatt atttctcgaa tttgtgtgag atttcgtctt atttttttga
    42421 agaagctctt ttgtcgcacc ccct
//
LOCUS       DACERL010000018        42051 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.018, whole genome shotgun sequence.
ACCESSION   DACERL010000018 DACERL010000000
VERSION     DACERL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42051
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.018"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            151..1014
                     /locus_tag="IXF54_000968"
     CDS             151..1014
                     /locus_tag="IXF54_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1404246.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1143..2765)
                     /locus_tag="IXF54_000969"
     CDS             complement(1143..2765)
                     /locus_tag="IXF54_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404247.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3014..3535
                     /locus_tag="IXF54_000970"
     CDS             3014..3535
                     /locus_tag="IXF54_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404248.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3721..5451)
                     /gene="spxB"
                     /locus_tag="IXF54_000971"
     CDS             complement(3721..5451)
                     /gene="spxB"
                     /locus_tag="IXF54_000971"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1404249.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5616..6434
                     /locus_tag="IXF54_000972"
     CDS             5616..6434
                     /locus_tag="IXF54_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1404250.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6537..6734)
                     /locus_tag="IXF54_000973"
     CDS             complement(6537..6734)
                     /locus_tag="IXF54_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404251.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6903..7481
                     /locus_tag="IXF54_000974"
     CDS             6903..7481
                     /locus_tag="IXF54_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1404252.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8526..9029)
                     /locus_tag="IXF54_000975"
     CDS             complement(8526..9029)
                     /locus_tag="IXF54_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1404253.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9388..10413
                     /locus_tag="IXF54_000976"
     CDS             9388..10413
                     /locus_tag="IXF54_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1404254.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10736..11614
                     /locus_tag="IXF54_000977"
     CDS             10736..11614
                     /locus_tag="IXF54_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404255.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12090..12206
                     /locus_tag="IXF54_000978"
     CDS             12090..12206
                     /locus_tag="IXF54_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1404256.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13134..13472
                     /locus_tag="IXF54_000979"
     CDS             13134..13472
                     /locus_tag="IXF54_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404257.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13566..13796
                     /locus_tag="IXF54_000980"
     CDS             13566..13796
                     /locus_tag="IXF54_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404258.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14196..14567
                     /locus_tag="IXF54_000981"
     CDS             14196..14567
                     /locus_tag="IXF54_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1404259.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14841..15533)
                     /locus_tag="IXF54_000982"
     CDS             complement(14841..15533)
                     /locus_tag="IXF54_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1404260.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15536..16321)
                     /locus_tag="IXF54_000983"
     CDS             complement(15536..16321)
                     /locus_tag="IXF54_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1404261.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16558..17958
                     /gene="lacG"
                     /locus_tag="IXF54_000984"
     CDS             16558..17958
                     /gene="lacG"
                     /locus_tag="IXF54_000984"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1404262.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17983..18300
                     /locus_tag="IXF54_000985"
     CDS             17983..18300
                     /locus_tag="IXF54_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1404263.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18313..19986
                     /locus_tag="IXF54_000986"
     CDS             18313..19986
                     /locus_tag="IXF54_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404264.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20000..21631
                     /locus_tag="IXF54_000987"
     CDS             20000..21631
                     /locus_tag="IXF54_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1404265.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22503..23693
                     /locus_tag="IXF54_000988"
     CDS             22503..23693
                     /locus_tag="IXF54_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ1404266.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23827..26016
                     /locus_tag="IXF54_000989"
     CDS             23827..26016
                     /locus_tag="IXF54_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1404267.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26013..27806
                     /locus_tag="IXF54_000990"
     CDS             26013..27806
                     /locus_tag="IXF54_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1404268.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28006..28320
                     /locus_tag="IXF54_000991"
     CDS             28006..28320
                     /locus_tag="IXF54_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1404269.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28341..28718
                     /locus_tag="IXF54_000992"
     CDS             28341..28718
                     /locus_tag="IXF54_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1404270.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28728..29501
                     /locus_tag="IXF54_000993"
     CDS             28728..29501
                     /locus_tag="IXF54_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404271.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29523..30926
                     /locus_tag="IXF54_000994"
     CDS             29523..30926
                     /locus_tag="IXF54_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1404272.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31108..32292
                     /locus_tag="IXF54_000995"
     CDS             31108..32292
                     /locus_tag="IXF54_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1404273.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32289..32579
                     /locus_tag="IXF54_000996"
     CDS             32289..32579
                     /locus_tag="IXF54_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404274.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32576..32797
                     /locus_tag="IXF54_000997"
     CDS             32576..32797
                     /locus_tag="IXF54_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404275.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32839..33618
                     /locus_tag="IXF54_000998"
     CDS             32839..33618
                     /locus_tag="IXF54_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1404276.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33685..34179
                     /locus_tag="IXF54_000999"
     CDS             33685..34179
                     /locus_tag="IXF54_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1404277.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34235..34873
                     /locus_tag="IXF54_001000"
     CDS             34235..34873
                     /locus_tag="IXF54_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404278.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34945..35340
                     /locus_tag="IXF54_001001"
     CDS             34945..35340
                     /locus_tag="IXF54_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1404279.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35324..37777
                     /locus_tag="IXF54_001002"
     CDS             35324..37777
                     /locus_tag="IXF54_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1404280.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37774..39801
                     /locus_tag="IXF54_001003"
     CDS             37774..39801
                     /locus_tag="IXF54_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404281.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39798..40820
                     /locus_tag="IXF54_001004"
     CDS             39798..40820
                     /locus_tag="IXF54_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1404282.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40837..41766
                     /locus_tag="IXF54_001005"
     CDS             40837..41766
                     /locus_tag="IXF54_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1404283.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT        13771 a         6959 c         8313 g        13008 t
ORIGIN      
        1 ccttcttatt tcttgaagct gaccacttct gtcacaacct cttattttga ttagaacttg
       61 cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt tgctataata gacgataagg
      121 gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt
      181 gatctgccat accatgtatt agcaaaagaa aaagttgatt ttttatcaat gaatctccaa
      241 ttgaatgatc aagagttagt agatgacctc ggaaagacat tcaattatga ccgctacatg
      301 aatcagctga aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg ccgatattta
      361 gaatttttcc gttcttatgc ggaaaaacag attcctgttt tatatgtggc tttttccagt
      421 ggattaagcg gctcttacag tagtgcgctt caagcggttg aattgctgaa ggaagaatat
      481 gaagaccctg agatctatgt ttttgataca aaagctgcaa gtattgggga agggctttta
      541 gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg
      601 ctggaaagta attatttgaa agtccattca tgggtaacag tagacgatct gaaatatcta
      661 gaacgaggcg gtaggatttc caaagcaagt gctactcttg gcagtctatt gagtgtaaag
      721 cctattatta cggtggataa agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga
      781 aacaagtcga tccagacaat cgtcaatgag acggtaaaaa caatcgttgc accacttgat
      841 caaatcgttt atgtggctta cgctggggat ctagagagcg cggagaaggc aaaacggctt
      901 ttggaagagc agataaagat gaaagatgtg aagctttatc ccttaggacc aacgatcgcc
      961 agtcacacag gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag
     1021 taactcattc tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga ctcttaaaag
     1081 atagatgagc atcctatctc ttagaggtcg ctgttttttt attttttaag ctaaataggc
     1141 cattatgccc ataactcgtt caattgtttt tgaatatctg cattttccaa aaattcatca
     1201 tatgtcgtat cagctcgatc gatcacacct ttatccgaaa cggcaatgat gcggtttgcc
     1261 aatgtttgga taaattgatg gtcatgtgac gcaaataaga ttgagccagt aaatgccatc
     1321 aagccgtcat tcaatgctgt gattgattct aagtctaaat ggttcgttgg atcatctaat
     1381 acaagtacgt tagctttcga aagcatcatt tttgaaagca tgcaacggac tttctctcca
     1441 ccagataaaa cattcactgg tttcaatact tcttcaccag aaaacaacat tcttcctaag
     1501 aagcttctta agaaggtgtt atcatcttct tctttacttg catattgacg caaccagtct
     1561 aaaatcgtta gatcagtatc gaattctttc gttgtatcct ttggtaaata agaattactt
     1621 gttgtcacac cccaacgtac tgttccagta tctggcgtaa tttcacccat gataactttg
     1681 aatagcgttg ttgttgttat atcattttct gcgacgaaag cgaccttgtc ttctttgctc
     1741 aaattaaaag atatatcatc taaaattttc tttccatcaa tcgttacact tacattttct
     1801 acctgcaata agtcgtttcc aatctcacgt tcaggtttga atccaacgaa tggataacgt
     1861 ctagaagaag gttggatatc gtctaaagta attttttcaa gcattttttt acgtgaagtt
     1921 gcttgttttg attttgatgc attcgcacta aagcgggcaa taaattcttg gagttctttg
     1981 atttgttctt cttttttggc atttgcgttt gcttgaagtt tagccgctag ttgactagat
     2041 tccaaccaaa aatcataatt tcctacataa agtttgattt taccaaaatc tagatccgcc
     2101 atatgtgtac atactttgtt caaaaagtga cggtcatggg atacaacgat cacagtattt
     2161 tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt ggatatccag accgtttgtt
     2221 ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa taacactttt
     2281 accttttgtc caccagttaa ttcactcatt tgctgatggt gcaattcttc agggatgttc
     2341 agaccttgca gtaaaacagc tgcttctggt tcagcttccc atccattaag ctcagcaaat
     2401 tctccttcta attcagctgc tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa
     2461 atcgcatctt tttctttcag cacttcatac agacgcttgt gtcccatgat gactgtttca
     2521 agtacagtat agtcttcata gtcgtaatgg ttctgtttca atacggccat ccgttcatct
     2581 ggtcccatag taacggaacc tgtggaaggc tgcagatccc ctgataacac tttaaggaac
     2641 gttgatttac ctgcaccatt tgcaccaatc aagccatagc agtttccggg ggtaaattta
     2701 atattcacat catcaaatag tttgcggtct gaaaagtgca aactcacatc atttacagta
     2761 atcaattttt ttcctcactt ttatcattat tagttttgac aagtcttata gaattatata
     2821 ggggaaaggt aaaagattca aggcaatcga acattctttg tgtttcacgt gaaacagaat
     2881 gaaaaaaata aaaataaaag cggagcgaaa atagctacta taggatttat tagtttgcaa
     2941 cagcataatt tttgacgtta tttataagag ggagtactat aaaactgtaa aagaaatgaa
     3001 agaaggattg aaaatggatg aaaaaagaac catcgactgg ggctcattga ttttgggaat
     3061 tttatttgta cttgtctcat tgttatcatt tcaagaccct gtcggaaatt tagttgcaat
     3121 tgtagtagtc tttgcgattt ttgcctttat taaagggata tttgaactgt ttgtacggaa
     3181 cagattaaaa gagctaacag gatataaagg aaagatgccc ttaataatcg ggatcatcga
     3241 tattctagtt ggcgtctttt tcttattcaa tattggtgca ggagtcgcag cattgccttt
     3301 tgtttttgca gtatggttta ttgctgattc agttttagcg ttatttactg ctgacttggc
     3361 aagaggagta agtgaaggat attattggtt tacgatcatc gtcaatattt taggaatttt
     3421 attaggaatt ttcttattgt ttaatccaat ttcttctgcg ttgacattaa gctttttagt
     3481 tggattctat tttatgttat ttggtatcac acatatcgtc tatgcattta gataagtaaa
     3541 atcaaacaaa taaaaaagcc ggcttccgat tacagtttta ctgttgtaat tggaagccgg
     3601 cttttttatc atggacaaat atgcacgagg tgatttttag gtgtcgagac aaatttatgc
     3661 aaaaatgtga gaagctatat acacaaattt tatcttaata ctcgaagcat agcgcagttg
     3721 tcacaacctc ttttcatata attcattcaa tactggcaaa tcatggactt catatttttc
     3781 tttgaatgct tggatttctt cttcagaata ttttcttgga tctaaatgca gatcttctac
     3841 tggcaatggg cgcttgttat tgatcttcac atcgattact acaggacgat ctgattttgc
     3901 agctgcatca aaggcaaatc gaagttgcga gtattctgtg actgtaaagc tatttgcccc
     3961 gagcgcttct ccaactttac cgaaatctgc atcttctaaa aatacaccaa atttcttttg
     4021 ttccgtatct tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa agacaacatt
     4081 aataattgga agctgatatt tgacttgtgt aatgatatct tgcatgacca tagcaaaccc
     4141 gccatcacca cttaaggtaa atacttgttt ttctgggtag cttagttgtg cggcaattcc
     4201 tcctggcaca ccattaccca tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc
     4261 gttcatattc aataaacgaa tagaatggat agtcgtgtta ccgacatctg tgacgaaaat
     4321 cgcatcgtct tcagcaatac gattgatttc tttatagaca gcctctggac gaatcggttc
     4381 ttcctctcga tcttcaaaag atttcaacca atttacccat ttttctttat tttcttgatt
     4441 tgctaggtac cagctatcag cagggcgagc atcccctaac tcgacaagtc tttgcaaagc
     4501 tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc
     4561 atcgatatcg atttggataa attcagcagt tggattaaaa aatgcacgtc caaatgggaa
     4621 gtcgctaccg acaaataaga tcagatcggc ttctgccaat gcttcatttc cgggtttagt
     4681 agcgacacgt ccagcaaaac caagaaagtt ttcataatca tcaggaataa ttccttttgc
     4741 tagaacggaa gcgacaacag gaactgaaaa atgttctgag aattctttga tttgcggaaa
     4801 tccgtttcgc gttccttgac caacataaag aactggtttt tttgcttttt caagataagg
     4861 caaagcagct agcaaatctt tttcttctgg taatatcaca cttgttttgt gagtgtgagc
     4921 ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga tcaacaggga tagtcacgat
     4981 agccacgcct ttatgctcat aggcggcttt aatggcttca tctactacat gcggaagact
     5041 ttgtggtgtc atgactgtac gattgtatac gctaacatct gcgaaaatag gattttcatt
     5101 tagttcctgg aaagagttat agttcatgga tgtagaagca acttgtccta agaacgctac
     5161 tagaggcaca tggtccatct gtgcatcata caaaccattg atcaaatgtg tcgcaccagg
     5221 tcctgcagag ccgaagacag cgccgacttt ccccgttaat tttgcatctg cagcggctgc
     5281 gagtgcgccg acttcttcat gacgaacttg aatgtagttg atttcggttt tttcttgata
     5341 tagcgcatcc ataattgaat tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc
     5401 ccagctttca agaactttta ccattgcgat tcctgcattg atttttgaca tggtgtttca
     5461 ctcctttagc aatgatgata cataaatttt ttgtatctag agtatagaac tttttatcga
     5521 aaatcccaac taattcgtct tttcacaaaa atgtagtcat gtttttggga atatgctaga
     5581 gtagtacata gatagattca agagagggga atggcatggt tgaatggcta ttgagacttg
     5641 aaccttggca gcaagcactt gctgggacag cttttactta ttttatgaca gctcttggtg
     5701 ccgggttggt ttttttcttt aaagaaatca aaaaagaagt gctaaacttg atgttaggat
     5761 ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt gttagatccg gctattacaa
     5821 aagcagaaga aaatggagat attgcttggc tagttgtaag tattgggttt ggtttaggcg
     5881 gtgtgttttt atatatggca gataaaacac tgccccatat gcattttgga ccacaacatg
     5941 aagcagaagg attgcctacc catttaaaac ggacgatctt gcttgttttt tctattacat
     6001 tgcataacat tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg
     6061 ataatccgac agccgctata ttagccgctg tttcggtggc gctaggtatt ggtatccaaa
     6121 acttcccaga aggagcagct gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa
     6181 aggcctttgt ttacggacaa gcttctggaa tcgttgaacc aattgctgga gtgattggag
     6241 cgctactagt aacgaaagtg gagctgctgc ttccttatgc acttgctttt gccgcaggag
     6301 caatgattta tgtcgtggtg gaagaattga tccctgaagc acagcagaca ttgtccagca
     6361 aacgtcattt tgcagttttt ggcgtcatga gtggatttat cattatgatg attttggatg
     6421 tggcattagg ataaataaaa aaaatatata tgatatatca aatagggccg cgaacttata
     6481 tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat catacattaa
     6541 taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg
     6601 attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga
     6661 ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata
     6721 tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac
     6781 agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga
     6841 tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata
     6901 tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta
     6961 ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag
     7021 gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc
     7081 atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt
     7141 tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca
     7201 atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt
     7261 atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga
     7321 tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct
     7381 tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt
     7441 ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat
     7501 ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt
     7561 caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc
     7621 acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga
     7681 tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat
     7741 ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt
     7801 ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg
     7861 ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt
     7921 gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg
     7981 gtgtggtttt tggaataaaa aaataacata tctcatttta cacttttgtt attttatact
     8041 taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta
     8101 ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa
     8161 tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta
     8221 taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat
     8281 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
     8341 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
     8401 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
     8461 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
     8521 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
     8581 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
     8641 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
     8701 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
     8761 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
     8821 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
     8881 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
     8941 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
     9001 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
     9061 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
     9121 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
     9181 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
     9241 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     9301 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     9361 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     9421 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     9481 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     9541 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     9601 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     9661 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     9721 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     9781 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     9841 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     9901 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     9961 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
    10021 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
    10081 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
    10141 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
    10201 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
    10261 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
    10321 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
    10381 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
    10441 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
    10501 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
    10561 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
    10621 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
    10681 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
    10741 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
    10801 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
    10861 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa
    10921 ggcgactgca aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat
    10981 ttatctatat gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag
    11041 aactatctat ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt
    11101 aacaggaagc tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat
    11161 ctttcggatt ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct
    11221 aagattagaa ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca
    11281 gaataagata agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga
    11341 ttaccattac caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg
    11401 gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga
    11461 tgaacaggac gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga
    11521 ttatccagaa agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca
    11581 cattggctct ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa
    11641 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    11701 gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
    11761 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg
    11821 ttctatcgaa cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt
    11881 taacggacaa gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag
    11941 agataggagt caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac
    12001 ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact
    12061 acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc
    12121 gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg
    12181 tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa
    12241 aacgtttttc ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg
    12301 ttgattttcc atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc
    12361 attccctaat tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa
    12421 gtattatctg tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg
    12481 atcactttac cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa
    12541 cgtaactaca taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga
    12601 gcaatacggt tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc
    12661 tctaacgttc agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt
    12721 ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa
    12781 aaagcagtta ctattgctag aataacatca aggaataata agttttggtt ttcagataat
    12841 agtcgataag ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt
    12901 tgtgagaatc actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag
    12961 tagaatgtaa atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga
    13021 ataagttttt caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata
    13081 ctttgttgat tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc
    13141 aaccagtttc aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat
    13201 atcaggaaaa tattgttccg gagactccat tagctagtga gccaattact gatattgatg
    13261 gaaaccaaat aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag
    13321 taaaaaatac agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt
    13381 atatttatac tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg
    13441 ctactgataa aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga
    13501 aattgatgaa aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat
    13561 tcgatatgct ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta
    13621 gagaagatga aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga
    13681 catttacagt ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg
    13741 gaatgtcttt agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga
    13801 gttcttatta tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg
    13861 ttaatattac taactttaat gcagtataaa gagtatccag agtattttat tttaactttt
    13921 gtatatttag taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct
    13981 aaagtttgtt tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt
    14041 aaaaatatta tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta
    14101 ctgataatga aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt
    14161 ttcaagatta ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa
    14221 ctcaagaagt catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag
    14281 aagcagttgc cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca
    14341 attatggcga taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg
    14401 tgaaggcaga tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg
    14461 ggacagagaa agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc
    14521 atttttcaac tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag
    14581 cattaataaa agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat
    14641 aatgtgaaaa cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta
    14701 aaagaatgaa aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct
    14761 ttcatgtcgt ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct
    14821 ttggtagtta gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa
    14881 aaaccagtaa aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt
    14941 ttgtttttac cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca
    15001 agtaatcctg agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg
    15061 tctgccgctt taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag
    15121 acaatcttgt cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca
    15181 ttctctttga cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca
    15241 agagtcgctt caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata
    15301 cgataatagc tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct
    15361 gaaatgaaat gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc
    15421 tcgtctgata aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc
    15481 agatgagagt ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg
    15541 ctcctatagt ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt
    15601 gctcttgaac ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat
    15661 tataaaacac ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct
    15721 tacgggaatt atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga
    15781 tcgcttcgtt gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta
    15841 aattgtcgtt ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa
    15901 tcagtagaat ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg
    15961 taatgatccg aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg
    16021 gtcctaagaa aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt
    16081 ttttttcttg aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt
    16141 aggaaagact ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat
    16201 ctaaatctct gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg
    16261 taaggattcc ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca
    16321 ttgtcttcac ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat
    16381 tcgaacaaaa gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg
    16441 ttttctggtt tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt
    16501 acaatgcaat cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg
    16561 cttaaattac cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga
    16621 gccactaagg aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg
    16681 cgattcagtc ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg
    16741 tgtgaacgtt ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct
    16801 gatggagctg ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa
    16861 tgtaagaaaa gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt
    16921 ttgtttgacc atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc
    16981 attttttgtt ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat
    17041 ccagttgcaa ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt
    17101 acaaagatcg tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat
    17161 ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat
    17221 gcggcaacag acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc
    17281 cgtttcttat tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt
    17341 gatgagatat gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa
    17401 ttgaaaaaag catccacgcg taatgactac ttagggatca atcactacca atgccatttt
    17461 gtcaaagctt acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc
    17521 tctgtttata aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact
    17581 gattgggact ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc
    17641 gattacccac attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag
    17701 tttgaagatg gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa
    17761 tccttaagca aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt
    17821 atggatcttt tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat
    17881 tttgaaaccc agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa
    17941 accaaaacga ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta
    18001 caaatgcttg gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa
    18061 ttattgaaag aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc
    18121 gacgaaaatt taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct
    18181 gggaaagaga tggagatggg attcattttt atccatgggc aggatcattt aatgaccact
    18241 ttattgctga gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag
    18301 gagaagaacg ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa
    18361 aaaatctcac gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca
    18421 gctattttat tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac
    18481 caatggcctg aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt
    18541 gtagggttac tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc
    18601 ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc
    18661 ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag
    18721 ggactactgg cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg
    18781 ctgaaagata ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc
    18841 cgagatattt ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt
    18901 cgacaaatct tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt
    18961 acagcagctg acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg
    19021 tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc
    19081 aatgtggaaa ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg
    19141 gtgggtttag ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct
    19201 tttatcttcc tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt
    19261 attccagtaa gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac
    19321 ccttattttt ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt
    19381 tttgtcgatg tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca
    19441 ccgatcggtt tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt
    19501 ctaatcgtcg ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta
    19561 gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat
    19621 gtacgtgagg aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc
    19681 aatgtgttgg tactttgtgc tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa
    19741 aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat
    19801 tacgaaatgc tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat
    19861 gatgatatca aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca
    19921 gaatatatcg gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt
    19981 caataaagga ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc
    20041 agctgtgtta ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt
    20101 tattcgcgga gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta
    20161 tgaagacgat atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt
    20221 tcgactgcgt ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac
    20281 aaatgatctg gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt
    20341 gctgctggat tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa
    20401 agcatggcaa ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa
    20461 cgttctgtcg gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga
    20521 actgaatagc ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt
    20581 tgatcgattg gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa
    20641 aacaactcca gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg
    20701 gtttgatgaa atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc
    20761 gtattggcac ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta
    20821 tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac
    20881 tatgccaaat gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac
    20941 aggacaagca gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg
    21001 cggggcgggc ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac
    21061 acaagcaggt atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca
    21121 agcgatgttt gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg
    21181 ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc
    21241 ttccaattgg caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc
    21301 gggcactttt tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc
    21361 agcttatcaa aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat
    21421 gtctggggga gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact
    21481 aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat
    21541 tacaacaggt gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa
    21601 cgttgatttt gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga
    21661 ggttcccttc agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt
    21721 gccaaaaata accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac
    21781 ttcatctaca ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa
    21841 aaattgcaaa aagttgtgca gataacataa agtttgttta agaaagggta accttagaat
    21901 tccgtcgtga atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt
    21961 cgtttttcga gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct
    22021 cttttggtag ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat
    22081 gttgatttat agggattccc cctccatatt atgttggctc tttattccca ctctgttttc
    22141 aacgtaaagt gtataaaaca gaatgcgata ctatcaaggg agttaaaaca atatacagaa
    22201 cagtaaaaca ctataaataa aaataaaagt gcaataaaag tgcaattttt gcacctaatc
    22261 atactctgtt tatatatgaa aactagattc tgaatccaat atttggttaa cgagaggcgt
    22321 tcgaatttga acgtctttta tatattactt tcaaataaat atgaggtaga atactatcta
    22381 ttttcttttt aacccaattg ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa
    22441 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaga gaggacttcc
    22501 ctatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg
    22561 aattattccg tgtccatttg gagaaagcag tcaatacgct tctcaaaacg gagttaacgg
    22621 ctttcctaga ttacgaaaag tatgatcgca ttggttttaa cacgggtaat tctcgtaacg
    22681 gctcctatga ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg
    22741 accgcaatgg tgagttcaag caacagactg ttcctgctta tagacggacg aatgacacgt
    22801 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
    22861 tgattgagaa aatgtatggg cattactaca cgccccaaac catgtccaat ataacaaaat
    22921 catttacaga agaggtaacg gcgtttaatg ggcgggagct tcatgaccgt tatgctgcta
    22981 tttatatgga cgcaacgtat attccgttaa agcggaaaac cgtcgccaag gaagctattc
    23041 atatcgcagt tggcattcgc ccggacggat caaaggaagt attgagctat gcgattgcac
    23101 cgactgaatc catcacgatt tgggaggaaa ttttattgga ccttcaagag cgcggtttga
    23161 aaaatgtcct cctgttcatc acggatggct taaaggggat ggtaggagcg attagtcggt
    23221 tctatcccaa agctcgtttt caacattgtt gtgtacacgt ttcccgtaat atcagtcata
    23281 aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcct
    23341 catctaaaga ggtggcgttg gaagcacgtg gtacttttgc ggaaaaatgg aaaaccagct
    23401 atccaaaagt ggttgaatcg attctttcga acgatcactt gctcactttc tatgatttcc
    23461 ccttggccat acgcaagagt atttattcta cgaacttgat tgaatccttt aataaacaaa
    23521 tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct
    23581 tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc
    23641 aacaggccga aggcgagctc gaacaaatgc taagccaact gattgagaat tagaaacaat
    23701 ctgcagtagg gaagaatcat ttacacaaaa ttattgacgc tcccttataa tgagaacttt
    23761 tactaacata atttatgata aacttactat ataatcatat taattaaata gttaggagat
    23821 ctgcttatgt ttatatctac tcttcaaatt cgaaatttta gaaattatga atccgaaatc
    23881 tttcgattcg ataatgagac aaatactatt ataggtgaga atgattcagg gaaaaccaat
    23941 gctcttactg cattgagaat cttattagat gatagttatt actatagtag taaaacacta
    24001 aaagaatcag atttttttca tggaattcaa aatgggtggc aagggcattg gattattatc
    24061 tctgcgacat ttgaaggaat cagcgaagaa gaatttgaca atgagatttg tgcttccatt
    24121 tctttaaatt ctgaaagtca aacaatctta gaagaattga tttcaaatgc agataaaggt
    24181 gtaggtagta tttctctatt tattagacct aacaaagcta ttagaaagca attatttgat
    24241 atttccactg aattagactc tcatcaattt aacgaattta gaaattctat tcgtctatcc
    24301 gactatgaat tttactacac atctaaatca aatctaaatt tctgtatcaa tgaaaattat
    24361 gataaattgg taggaagact caatgacaat atagctagta atcctgaaga agacgatgaa
    24421 gctctgttgg gctcaagaat tgacatgtca gatattttca agcatatttc ggttgtttat
    24481 gttgacgctc ttcgagatgt tcttagagaa atgaaaaaca atcgaaaccc agtcaaaaga
    24541 atcatggaaa caattgaatc aaaaatatca tcagataatg ttgagtcatt aaaaacaatt
    24601 atacagcaat taaatgagac tattaccagt gttcctgaaa ttaggaaaat tggcgaaaat
    24661 attaatcggc aactaaatag cataattggt tcagtgtatt ctcctaatct tcttctttcc
    24721 tcaactatgt cagatgatat gggttcttta gcaaaatttc tttcaatgaa gccagaacaa
    24781 aatatcgact tagacttact agggttagga catctaaata tgatttattt agctttgaaa
    24841 atagtcgaat ttgaagcgtg tcgttcaaga gaactactta atattatgtt gattgaagaa
    24901 ccagaagctc atattcatca tcatattcaa aaaactctgt tcgaaggatt aaatcttcaa
    24961 aaaaactata ctcagattct catgaccaca cattctgtac atttagctga agtgtctgaa
    25021 atatctcgga tgaatgtatt aaaatcgttt aatggcaaat ctatcgttat gaatccagca
    25081 aataatctga actcatttgc taaagaaaag ttaaaaaaga acaatctaaa tctaataaac
    25141 gcagtagaga gatatttaga tgttaaaaga aatggattgc ttttttctaa aggtgttatc
    25201 ttagttgaag gagatgctga agaaatactt attcctcaaa tagtaaaaaa attatttggt
    25261 ataaacttag acgaacttgg aataggtttg attaatgttg gtagtacttc gtttgaatat
    25321 atcgctagct tgtttgacga tgatagaata cagagaaagt gtgctattgt aactgattta
    25381 gatgaacagg caatcccatc agaacatcga ttgtataaaa gcactgcaca agagaaaggt
    25441 caagcaagaa aagaaaaatt atcctccctt tttaatgaaa acaattgggt taattgtttt
    25501 tatgctaata caacatttga aattgagttt ttagatgtga ataaagaaaa tattctaact
    25561 tatgtcagtc caattataga tgacgcattc aaagataaaa aaacaataac tgcttataaa
    25621 gaagaaatac aaagtgaaca atatttagaa agaaatttag cgatgttaaa actagccaat
    25681 catgttggta aggggtggtt tgcaattgag ttaggtgaaa aaattgactt tttaatcaaa
    25741 attcctaagt atattcttaa tgctattgct tttgcatgtc aggaaatagt agatctctca
    25801 atttataaaa aaattctact ccatacaatc gacttgtacg aggaaactac agaattgtca
    25861 gctttatcag ctagtttaaa aacaagtgat acagctgatt tgaatatagc aataatttct
    25921 ttattagagt actttggtaa agatgaggat gttgtgtatc taatcaatca gatagaaaat
    25981 tacacaaaaa tactttatgc tgaggtttca aaatgacaaa tatcttcgaa aaaatcgtaa
    26041 atgatttaaa tttagaacaa aaagatgttg tggaaagtga agataacctt tatgtaaatg
    26101 catgtcctgg gagtgggaaa acaagggtac ttacaaggaa aattgcttac caagcaatta
    26161 aacacgatgg atcactaaaa agaataattg ctatcacata cactaataga gctgctgaag
    26221 aaattaagga acgcctaagt ttacttagta tagatgatga tattaatatt tgggtgggga
    26281 caatacatca gttttgtctt gaatttatta tttatccttt taaaatgaat ctctcaagag
    26341 tatcaaaagg atttacaatt attgatgatt atactcaaag agcttacgtc agccaaatta
    26401 ataatatgct gaatttaaat ctcaaaacgt atgaactgaa caaaattgat ttgtcacttt
    26461 ctcatgattt taaaattaat gaagttcagt atactcaagt ttctgaaatg tatcacacta
    26521 tgttggaaaa aaataaagaa attgattttg atatgatttt actagtagcg tataaaattt
    26581 taactgtaaa cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg
    26641 atgaatttca agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca
    26701 accctcaaat tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag
    26761 atggtatagc taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa
    26821 agacgctaca tggctgttac aggtctaatc agagattagt agacttctac tctaattttc
    26881 agagttgtcc ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa
    26941 gttatgattg caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg
    27001 aaaagattga aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc
    27061 ctctgttttc actgggtgag tatttaaaga aagaattacc gtattgcaat tttgatgcta
    27121 taaatatatc accaattaag gtaaataatc atagtttatt tttcaagtta gcaattttgt
    27181 actttactga atcaggtgaa aaggttcgaa gaagaaagct aattgcaaac gatattttaa
    27241 ttatattaaa aaatgaattt ggaatagaag ttaaagatta ttttattgac ttagatttat
    27301 tacagctaat aaacagtgtg aaaagaatgt atagtggaga cgacaacggt gttgatttat
    27361 acaaatttgt taccaaacat atatttgtta atcttaatat tgacgagaaa aattacccgt
    27421 ctatttttaa gcactatcta ttttttatta acgaagtaga agatcggata aaaaataatt
    27481 cattatctag tagtgtatct agttttaaga agatgtttaa atctcgagat ggtatcacac
    27541 ttactacagc acataaagtt aaaggaaatg agtatgatac tgttatcgct attaatcttc
    27601 taaacgggaa aattcctcat tggaatgaaa tttttaatgt ttctgataaa ggaattagtt
    27661 cagcgagaaa actactatac gtaatttgtt caagagcaaa aaataattta tttcttttct
    27721 cagaggtggg ttattttacg agtaaaggag acttgcttta tcccactcaa ttattagata
    27781 aaatagaatt tgattataat atttaaaata cctaggtagt cgttgtttta aatatagtag
    27841 gaatcggacg ctcagtacgg agcgtctatt tttttaccca attttgaaag gaagtgaact
    27901 tatgaaaata aaaaatcaag aatcaaaagg tcgctctccc ccctttaaaa ccatcacaca
    27961 aataagattc attcaaacat aaaaacagaa aggataggta aaaatatgga acttaaatat
    28021 gtcattccca atatggaaaa aacatttgga caattagaat atgctggtga agggaacatt
    28081 gaacaacgtc gaataaatgg cagaccaacc attctctctc gtagttacaa tttatactca
    28141 acgattcaac gagcagatga tattgttgtc attcttccct ccgaagctgg cgagaaacat
    28201 tttgaatccg acgaaaaagt caaactcatc aacccacata ttaccgcaga aggttataaa
    28261 attggcaatc gaggatttac caattacatt atgcacgcag acgatatggt gaaagcataa
    28321 gaaaggaaag gtgaaaaaat atgagattag cagaaggtat tgtgattgaa aaagaccaga
    28381 catttggcac attgaaattt tccgccttgc gtcgtgaggt tcgtatccag aatgaagatg
    28441 gtacggtttc cgaagaaatt aaagaacgta cctatgactt aaaatcaaaa ggacaaggac
    28501 gcatgataca agtcagtatc cccgcttctg ttcctttgaa agaatttgac tacaacgcag
    28561 aagtggaatt aatcaatcct gtggcagata ccgtagcgac ggctactttt cgaggggcag
    28621 acgttgattg gtacgtgaaa gcagacgatc ttgtattaaa aggacagaca aaacctcatc
    28681 agaaatataa aaaagaagcg tccgaaagtg aaaaataggt gttgcttatg acaaacacac
    28741 cattgatttt gaaagaaatt ccgaaggatg aagccatttc ttttattcgg caataccatt
    28801 attcaaagat actccctaga ttgtgtaaat actttctagg gattttttca gaagaaaaat
    28861 tattaggcgt tgtggaatta ggctggggta cgcaacccct acaaaccata cgcaaactct
    28921 ttcccgacag ttcccttcaa accacagatt atttagaaat tggaaagatg tgttttcttc
    28981 cagaaatgaa tcaaaccaat tattttggca gtcaagctct ttcagctcta attaagtggt
    29041 tgaaagaaca tacggactgt cattttttat atacactggc agatggaatt gagggaaaat
    29101 gtggctatgt ctatcaagcg tccaacttct tttactgcgg gtatttcaag acaagtgtct
    29161 atcgtgataa gcagtcatgg gaaaagatac accctcgaag tgctcgcctt ttattagagg
    29221 aaaatgctcg ctttgaacag gtagagaaaa agcattggct ttcccaggca ttttgcgaat
    29281 acaaagggat tgagaaaatc aatggacgca tgtttcgcta tctctatccg ttgacaaaag
    29341 aagcgaaaaa actgttagga cacactctct atcgacgtca ttattatccg aaagaaaaaa
    29401 atttacgctt tgaaaagcga atcgcttatc agaaatatga agccatttct caaccaactt
    29461 ttgataaaca ggctcgtatc tacaatacac aattattttg ataggtggaa aggaggattt
    29521 ttatgccatt gcttcatgac agaggagagc gaatcaaagc caacgataag cacttggtct
    29581 atcattttgt actaatttca ttgctggtag tttttctagt cgtcctactc ccttttcatt
    29641 ggagagcgtt acaatcggtg aactggcaag cagttcagtt agatacattt ttctatcaac
    29701 ttcatacgcc atacttttgg agcagtattc tcactgctct ttttgtatgc ggacttatca
    29761 gctggcttta ttatcactat cgtatagatt ctataaaaca gttagagcac cgtcaaaagt
    29821 tagctcgtat gattttagaa aatcattggt atgaagccga agaaactcaa tcagagggat
    29881 tctttaaaga tttacccagc aagaaaacca aacaacgaat ccgccatttt cctaaaatat
    29941 attatcgaat gaaagatgga ctaatccata ttcatgtaga aatcacctta gggaaatatc
    30001 aagatcagct tttgcaccta gaaaacaaac tagaaacagg attgtattgt gaattagtgg
    30061 aaaaaacgtt gaaagattct tatgtagagt atgtcctact ttatgatact attggcaatc
    30121 gaattggcat tgaagacgtg gtagctcaag atggacgtgt caaattgatg gaatcggtct
    30181 attgggagtt cgattctctc cctcacatgt taattgctgg gggcactggt agtggaaaat
    30241 cctattttat tctcacattg attgaatctc tcctgcatac aaatgcaaag ctttatattc
    30301 ttgaccctaa aaatgctgat ttggcagact taggggcggt catgaataat gtctattacc
    30361 gtaaagaaga tatgttggca tgtatcaatc aattttacga agatatgatt gatcgcagtg
    30421 aaaccatgaa acaacaccct aactataaaa caggagaaaa ttatgcttat cttggtttgt
    30481 ctgccaattt tcttattttt gatgaatatg tggcaatgat ggatatgcta ggtcgtgaaa
    30541 gtaccaccgt tattcataaa ctcaaacaaa ttgtaatgtt aggacgacaa gcaggcttct
    30601 ttttaattct tgcctgtcaa cgtccagacg caaaatattt tagtgatggg attcgggaca
    30661 actttaactt ccgtgtggct ttgggtcgta tgtctgaaat gggctttggt atgatgttcg
    30721 gaagcgatac acaaaaacaa tttttcttaa aacccatcaa gggaagaggt tacgtggata
    30781 caggaaaaag tgtgattagt gagttttata caccccttgt accaaaaagg tacgatttct
    30841 taggagaaat aggaaaagtc attcaaaaaa agcaatctga gcctgtaccg cacgaagtga
    30901 aaggtacagg ttcagacttg ctttgattgg tgtggcgtta gccacgccaa tcctttagcc
    30961 ccctctatct aacagggggg cacaaataca actgaaaaca ggcaattctc atcaaccaat
    31021 gttgatggta caaaggattg cggaaaatat gaaaggtgta aactcaaggg tcatagttta
    31081 catctttttt agattggagg tttgcgaatg gaaaacgaaa aaatttggat taaggaattg
    31141 aaagaaaagc gactggctta tggcgtttct caaaataaac tggcggttgc ttctcatatt
    31201 accagacctt acctatcgga tattgaaaca ggcaaggcag tccctactca acaggtgaaa
    31261 gaagatttac tgaacgcttt ggaacgcttc aatcccgata acccattgga gatgttattc
    31321 gactatgtac ggattcgatt ccctaccaat gatgtggctc aaattatcgg tgaggtttta
    31381 cgtttgaata tggattatat gctacacgaa gactttggct attattctta tcccgaacac
    31441 tatcgtttcg gtgatattgt tgtcctagtc tctcatgatg taagtaaagg tgttttatta
    31501 gaactgaaag gaaaaggctg tcggcaattt gaaaactttc tattggctca acatcggagc
    31561 tggtatgatt tctttcaaga ttgcatggaa cataaaggta ttttcaaacg cttggattta
    31621 gctatcaacg ataaaacagg gattttgaat attccagatt tggcaaagaa atgtaaacag
    31681 gaagaatgca tttccgtctt tcgaagcttt aaaaattatc gcagtggtga attggttcac
    31741 cgtgatgaaa aaccatacat gggaaacaca ctttatattg gctcattaaa aagtgaagtc
    31801 tatttctgta tatatgagaa agattatgag caatacgtga aaaatgatgt tcccttagaa
    31861 gacgcagaag taaagaatcg gtttgaaatt cggttaaaaa atgaccgagc cacccaagcc
    31921 atgaaagatt tattggcaca tcaacaggcg gaaaaaacag cctttgaaat tatcaatcgc
    31981 tatatccgct ttgcagataa agacgacact aaacgtcgga gtgattggaa aactaatgaa
    32041 cgttgggaat ggtttatcgg taaaaatcgt ggagcattac gcctaaccac acagccagag
    32101 ccttattctt ttgaacgaac attgaactgg ttacatcatc aagtcgcccc tacattaaaa
    32161 atcgcaagca ttttagatgt tctcaacggt acaaccatta tctccaccat gattcaggaa
    32221 gccaaactga cagaaaaaca tgagaaatta attgaacaac aacatctagc catggaggat
    32281 ttgattacat gagcctttta acaaaagaat taaagaagtt agggtttcag tgtggcattg
    32341 agtttcaagc gtatatacaa aatacaggga aatatacgtc tctcattatt gagggaaaac
    32401 ggcaagctgg tgataccatc tatacctatg atttttacaa ggtgagattt tataataatt
    32461 ataccaatcg agtaaccgtc tatggagagc atttaacccc ttttcaatta ttaaggcgtg
    32521 taaaaagcta tatctattat cgagaaaaat atcttaagga aaggaggaca atcacatgaa
    32581 ttttgggcag aatttgtaca actggttctt ggataatgca cagtcattgg tattgttagc
    32641 gattgttgtc attggactgt ttttaggttt taaacgagaa ttttcaaaac tcattggctt
    32701 cttgattatt gctctaatcg ccgtcggact tgtgttcaat gcagctggcg tgaaagatgt
    32761 gttattaaac ttatttaacc gaatcattgg tgcttaacag atatttttca tgataaaata
    32821 ggaagtatat attttgttat gaaaggattt atgaatatgt ttacaaatga tcaatttaaa
    32881 tctcttttaa ttaaaacatt agaaagaaag agctcttcaa attattatga aggaggtaac
    32941 gagattgttt ctaaaatgaa aatatcagag gtaactttag atgagactga cgattttact
    33001 tattataaag gatataaaaa gggctggaat acgctttgca catatcttaa aataagagtt
    33061 ccttttgatg atttagactt ttttgaaagt cataaagatt tgattaccca gacagcaagc
    33121 tccatatatg ataaacaagg tgataatgta ttagtggata ctattttagt accacttcca
    33181 gagaattacg aagtaattaa ctttagtcag ttgaaaatta gtgatgtggt ttcacaagct
    33241 attgaagacg ctgagtcttt tatgagtaat ggtgaatatc aacgtgcctt tgacagagtt
    33301 cacactgctt ttcatggata tttaattgaa atactaaaaa aatatgaaat tacagttcct
    33361 agagacgaaa atctatctaa attatatagt aggatacaac aattaattga aaaagaaatt
    33421 caacctactg aacttgctga tatagtgaag acaacgataa ggtcatcaaa tggaatgatt
    33481 agctctctta atgaagcaag aaataggcat tcacttgcac accctaatac aaatattatt
    33541 ggaaagagag aagcaaaact aataataggt atttcaagta cagttacaga ttatattagc
    33601 ggataccttg ataagtagcg tcattaactg attgctactt tttttatgac tatttttata
    33661 aaaaggatgt gataagaatt ggaaatgaaa gtctatgtgg caaatttagg acggtataat
    33721 gaaggtgaat tagtcggagc atggtttact ccaccaattg atgaggaaga aatggcagaa
    33781 agaatcggtt taaatgagga ctacgaagaa tatgcaatac atgattttga gcttcctttt
    33841 gatgtggacg aatacacgcc gattagtgaa atcaaccgtc tatgtgaagc cattcaagaa
    33901 atcgaaggta cacctattta taatgaactc aaagaaattc aaggcatgtg gtttagtagc
    33961 ttggaagaat tactggaaaa caaagaggat attcattgtt atagtgattg tgattcgatg
    34021 gaagatgtcg cccgttatta cgtggaagaa acaggacagc ttggtgaagt cccttccaat
    34081 ttacaaaatt atattgacta ccaatctttg ggacgtgata tggaaataga gggtaattac
    34141 ctagttacct ctcatggggt atttgaatat tgtcagtaag tttaacaatc cgtgttataa
    34201 ttaaattaat aaatagcacg gagaggtata aattatggta aatgtatttg aaaatcttaa
    34261 actattaaaa ctagacatga aagataaagg ctgggtgata gattcctttt actttcgtta
    34321 caaacagcaa aattatattg tacttgttaa gctatttgaa aaagaggaaa aagttccaga
    34381 atatgctctg ttaaaactag aatttttaaa ggaaaacgat ttttcagata tgctagctgt
    34441 ctatgctaat tcggtaaaat tatatacaga cacgaaaaca ataagagaat attttggtat
    34501 cgaatacagt agtaacttgg gagatgtttt atttcaattt agccaaactc ttgctcgatt
    34561 tattccgaca gaagttagtg agaagaaaaa tgaagaccaa aaagaagcca tgtgtttctc
    34621 attaagtcaa agtgattcag aagatcccag aaaaaaatat tgtttttcag ttagaagaaa
    34681 tcctttaaag ggaaatggtg agctgggaaa acgaagtcct tttaatgaca ataaaacgag
    34741 attgtataga cccagcttat atgaacgatt agggtcagat accaatttaa gtttcagata
    34801 ttcaatgaat ccagatgatg aagaaactga tgaaggtatt attgcaaaat ggacaaaaaa
    34861 taaaattgaa tagttaaagc attgaaagag caatcaccta ttttgattgt tctttttcta
    34921 tttttacgga aaggacgtga agacatgaaa aaaataagaa gttacaccag tatttggtca
    34981 gtagaaaaag tgatttatgc gattaatgat ttacaactcc ctttcccaat tacctttacg
    35041 caaatgacat ggtttatcgt ttctttgttt gtcgtcatac tctttggcag tttaccacca
    35101 ctttctctga ttgacggagc actactcaaa tacttaggga ttcccattgt tctcactggg
    35161 ttcatgtgcc aaaagacctt tgacggaaag aaaccctatg gatttctaaa gtctatcatt
    35221 acctatgctc tacgccctaa agtaacctac gctggcaaac aaatacgatt aaaaaaagaa
    35281 acaatgaatg aatcgattac gattgtaagg agtgaacaac tagatggcat atccgattaa
    35341 atatatggaa gataatctcg tatttaatca tgatggcgaa gtattcgctt attatgaatt
    35401 aattccctat aattacagtt ttttaagttc cgatgaaaaa attcaagttc atgataattt
    35461 ctgtcaattg attgcccaaa atcgtgatgg caagattcat gctctgcaac tagcaaccga
    35521 atcaagtatt cgagccacac aggaacaatc gaaaaaagaa atcactggac gattaaaaga
    35581 gatagcttac gaaaaaatag atgaacagac cgaagcgtta atttctatga ttggtgacaa
    35641 tcaagtagac tatcgctttt ttattggttt taaactctac ctcaatgaac aggaagtatc
    35701 cgtaaaaaat gttgggaaag aaattcaaaa agcatttgcg gaattctttc atggtgtgaa
    35761 tcacaaatta atgggcgatt ttgtttctat gcctatggcg gaaatcgaac gcttttctaa
    35821 gatggaacag ttgttagaaa gtaaattatc cagacgcttt cagattcgta aattggagaa
    35881 agatgatttt ggttatatta ttgaacatct ttacgggcaa acaggtgtcc cttttgatga
    35941 ctacgactac catttaccga aacgatacga agataaaaaa gtgttggtaa agaaatacga
    36001 tattctcaaa cccactcgct gtttaatcga agaaaatcaa cgctatttac ggattgaaca
    36061 ggaagatacc actacttatg tggcgtattt tacaattaat agtattgttg gtgaattgga
    36121 tttccctaat agtgaaattt tttattacca gcaacagcag tttactttcc ccgttgatac
    36181 cagtatgaat gttgaaattg tcgccaataa aaaagcctta tctaccgttc gcaataaaaa
    36241 gaaagaactg aaagatttag atagccacgc atgggaaagc gacaacgaaa ccagtactaa
    36301 tgtggtggac gctttagaat ctgtcgatga attagaaagt aatttagacc aaaccaaaga
    36361 atctatgtac aaactaagct atgtggtacg ggtatcagca aagaatttgg acgagctgaa
    36421 acaacgttgt gatgaagtaa aagattttta tgatgatctg tctatcaaat tagtacgacc
    36481 ttttggcgat atgctaggct tgcataatga atttattccc tcaagcaagc gatatatcaa
    36541 tgactatatt caatatgtca cgtcggattt tttggctggt cttggtttcg gtgctacaca
    36601 aatgattggt gaaaattctg gtatttacgt cggttacaat ctcgatacag gcagaaatat
    36661 ttatctccaa cctagccttg caagtcaagg aattaaaggc tctgtaacaa atgcattagc
    36721 gtccgctttt attggttcat taggtggcgg aaaatcattc tctaacaata tgctggtcta
    36781 ttattcagta ctctttggcg gacaggcaac cattgttgac ccaaaagctg aacgtggtga
    36841 atggaaagaa accttacctg atattgccca tgaaatcaat attgttaatc tcacgagtga
    36901 gaacgacaat aaagggttac ttgaccctta tgtgatttta aaactcccga aagattcaga
    36961 aagccttgcc attgatattc tcacgttctt aacaggtatt tccagccgag atagcgagaa
    37021 gtttcctgtc ctgcgtaaag ctattcggag tgtaactcaa agcaaacaac gagggttgtt
    37081 attggtgatt gatgaattgc gaaaagaaga tacgcccatc agcaatagta ttgctgacca
    37141 tatcgaaagc tttgtcgatt atgattttgc ccatctgtta ttcagtgatg gcagtgtcac
    37201 ccaatccatt agcttggaaa aacaactcaa tattattcaa gtggcagatt tggtcttacc
    37261 cgattccaat agctcttttg aagaatacac taccatggaa ttattaagtg tggcaatgct
    37321 gattgtcatt agtacctttg ctttagactt tatccactct gaccgttcca tttttaaaat
    37381 tgttgacctt gatgaggctt ggagtttttt acaagtggca caaggaaaag ctctatctat
    37441 gcgattggtt cgagcaggac gtgccatgaa tgcaggggtc tatttcgtta ctcaaaacgc
    37501 ggacgattta ctagatgaaa agatgaagaa taatattggc ttgaaatttg cctttcgttc
    37561 cactgaccta gtggaaatca aaaagacctt agaatttttt ggtgtggaca aggaagatga
    37621 aaacaatcag aaacgcttac gtgaattaga aaatggacaa tgtttagttt cagacatcta
    37681 tggtcgtgta ggggttatgc agtttcaccc gatttttgaa gagttgcttc atgcctttga
    37741 taccagaccg cctgtgcgaa atgaggtgga agaatgacac aaaaacaaaa gaaatttctt
    37801 cactatagtt ggataacgct cctttgtgtg ggcgttattt tacttttatt aggcacgctg
    37861 ggcaatgcag tacaagcgac aggcttagtt gatgaaacca ttgatacaag caatgaatac
    37921 tccaagtatg gtttgaatca atatcagctt gattattatg tggacaatag ctggggctgg
    37981 cttccatgga attggagtga tggcatagga caatcggtga tgtatggatt gtatgccatt
    38041 actaatttta tttggacaat ttctctttat ctatcgaacg ccacaggtta tttggtgaaa
    38101 gaagcctatg ccttagattt cattagtcaa acatcccaag caattggcga aaacatgcag
    38161 acgattgcag gcattacacc gaatggcttt tccagttcag ggttttatgt cggtttcctc
    38221 cttcttttta ttctcattgt tggaatctat gttgcctata caggattggt taagcgagaa
    38281 acgacgaaag tgatacgagc tgttttaaac tttgtcgtgg tgtttattct atcggcttcc
    38341 tttattgcgt atgcacccga ttatattgcg aaaatcaatg atttttccaa agatgtcagt
    38401 accgccagtc tgaatattgg gacaaaaatt gttcttccta acacggatag tcaagggaaa
    38461 gacagtgtgg acatgatacg tgacagctta ttctctgttc aagtaaagca accatggcta
    38521 ttgcttcaat atggctccac tgatgtagaa gcgttagggg aagaacgtgt ggaaagttta
    38581 ttgtctacca gtccagatac aaacaatggt gaagatagag aaaatgtagt gattgaagaa
    38641 attgaagatc atgacaatac ctatttaacc ctaccgaaga cgatttccag attaggaaca
    38701 gtcttctttt tgtttgtatt caatattggt atctcgattt ttgttttcct tctaacagga
    38761 atcatgattt tttcgcaagt gctatttatt attttcgcta tgtttttgcc aatcagtttc
    38821 ctattaagta tgttgccgag ttttgatggc atgggcaggc atgccattac caagttattt
    38881 aatgtgatta tgacaagggc tggtattacc ttgattatta ccgttgcctt tagtatttcc
    38941 actatgcttt attcgctgtc agcaagttct ccctttttca tgattatgtt tttacaaatt
    39001 gtgacctttg cagggattta ttttaaactg ggcgatttaa tgagcctatt cactttacaa
    39061 agcaacgact ctcaaaacat gggcagacaa gtgtttcgca gacctcgtca aatgatgaat
    39121 cgacaaacca gaagatttca acgcagtatg agtaagattt ttcgtggaaa agcagggtca
    39181 acgaagcaaa agacaatgac aacaaagcag aatcaaacca ataaagctaa ccatactcgt
    39241 caaaatgaac ggactacacc aaagaaagaa acagcacttt ccaatgtgaa aaagcaacaa
    39301 ggcggtatcg ggcaacgatt aggtgaaaag acagcccatg tattggacac gaaaaatcgt
    39361 ctagtcaata aaagaaagca ggcaaaacaa cagatacagg cgacaccaac ccatgtcaaa
    39421 tatgcccttc atcaaggaaa agagaatgtc actcataatg taacagactt taaacaatct
    39481 atggcagata ccaaagctca acgcaaacaa cagcgtatga accagcagga gcaaaaacga
    39541 aagaacattg ctcaaaagcg tttggagctg gataaagcaa aagaaaaaag acgaaaagaa
    39601 aacccatcat ctcctgttca gaataaccag cagcatgttc gcaagcgtca gcaaaccgtc
    39661 acgcctgttt tgtcacaaaa cactaaacgt gaaaatcatc agcgaccatt gaaaaaagag
    39721 caaaaacaaa agacaccacc gaagcaattc aatcagagga agctaaataa aagaacggat
    39781 aagcgaggac aacaaacatg aagctaaaaa taacagtgat ttgtagtgtg gtcttctctc
    39841 tcttcttttt tctcttatta ttcctagtca ttttctttgc agatgatacg gacagtggcg
    39901 aaaacaacaa ggattcttct attccacaag gcggtgtgac cgtttcacca gaagtgttgg
    39961 cacaccgtcc gttgattgaa aaatatggta aggaatatgg gattgaagac tatgtttctt
    40021 atatcttagc cattatgcaa gtggaatcgg gcggtacagc agaagatgtt atgcaaagtt
    40081 ccgaatcttt gggtttaccg cccaacagtt tgagtaccga agagtccatc aaacaagggg
    40141 ttaaatattt cagtgaatta ttaaccagtg cagaacaaca aggagtggat atagattctg
    40201 ttatccaatc ttataattat ggcggtggct ttttaaatta tgtgaggagt catgggaaaa
    40261 aatataccta tgaattagcc gaacagtttt ctaaagagaa gtcgggtggt caaaaagcag
    40321 actatcccaa tcctatagcc atacctgtga atggtggttg gcgatataac tatggaaatc
    40381 agttttatgt tcagttggtt tcacaatacc taaccgacac ttccccaaca gaatttgatg
    40441 atgaaaccgt gcaagtcatt atggacgaag ccttgaaata cgaagggttt ccctatgtct
    40501 ttggaggggc gtcgcctacc acctcttttg attgtagtgg cttaatacaa tgggtgtatg
    40561 acaaagctgg catttctctc cctcgtgtcg cccaagatca gtatgacgct acgcaagaaa
    40621 tctctatgga agaagcccaa gcaggcgacc ttatcttctt tcattcaacc tataatgcag
    40681 gcacatatgt gactcatgtg gcgatatatt tagagggaaa tcgcttttat catgcaggcg
    40741 acccaatcgg ttatggcgac ttaagcagtc gttattggca agaccatctg attggggcta
    40801 gacgtgttat tcataactaa gaaaggaatg aatcaaatga ttataaaaat cgagcgaaaa
    40861 gaaaagaagg agaaaccaaa gaaatctaaa aaaccgagca agcaaagaaa agttccgatg
    40921 attaaagttg gcacgcataa gaaattaacc tttgttttat gggttttatt gattggtagt
    40981 gtcggatttg gaatctataa aaattttact gccattgaca ctcatactgt ccgtgaaaca
    41041 gaaatcatta aacagcagat tgtcgatacc aaccaagtag aaagttttgt gaaatccttt
    41101 gcgaaagact acttttcgtg gcaacaatcc caagaagcca ttgataaacg aaatgaaaaa
    41161 ttgacccatt atttgacgga agaattgcag gtattaaatg aagaaatgat tcgtaaggat
    41221 attcctacta gttctagtgt gaacgatata caggtgtggc aggtttctca agtgaatgag
    41281 aacacctttg aggtactgtt ttctgttgaa caagttatta ccgaagacaa agacaaggaa
    41341 actatatcgt ctagctttca tgtggttgtc catatagatg aatcagataa tatggttatt
    41401 atcaaaaatc cgacaatgag taagaaacct caaaaatcgg actaccaacc caaacagcta
    41461 gaaagtgacc atacagtaga tacagaaacg atggacgaaa tcactagctt tttagaaacg
    41521 ttcttccagc tctatcctac cgccacagaa aaagaattga cttactatgt aagtaatcat
    41581 gtgttaccta tgattaacaa ggaatatgtt ttcgaggaat tggtgaatcc tatctttacc
    41641 agaaaagata accaagtaat cgtgaatgtc gctgtgaaat atttagatca agaaacaaag
    41701 gcaacccaaa tctcgcaatt tgagttgata ttagaaaaac aagataattg gaagattgta
    41761 aaataacaaa tattggtaca tgattacaga tactttgtaa tcatgtactc tttttgataa
    41821 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt gactatttaa
    41881 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagcggga
    41941 acaaataatt agatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaagaata
    42001 cctttatcat gtgattctaa agtatccaga gaatatctgt atgctttgta t
//
LOCUS       DACERL010000019        41501 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.019, whole genome shotgun sequence.
ACCESSION   DACERL010000019 DACERL010000000
VERSION     DACERL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41501
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.019"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1273..1938)
                     /locus_tag="IXF54_001006"
     CDS             complement(1273..1938)
                     /locus_tag="IXF54_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404284.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IXF54_001007"
     CDS             2064..4100
                     /locus_tag="IXF54_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1404285.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IXF54_001008"
     CDS             4104..4742
                     /locus_tag="IXF54_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404286.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IXF54_001009"
     CDS             4770..5198
                     /locus_tag="IXF54_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404287.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IXF54_001010"
     CDS             5655..6566
                     /locus_tag="IXF54_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1404288.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IXF54_001011"
     CDS             6783..7220
                     /locus_tag="IXF54_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404289.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IXF54_001012"
     CDS             7315..8199
                     /locus_tag="IXF54_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1404290.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IXF54_001013"
     CDS             8423..11590
                     /locus_tag="IXF54_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404291.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IXF54_001014"
     CDS             11610..12155
                     /locus_tag="IXF54_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1404292.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IXF54_001015"
     CDS             12183..12638
                     /locus_tag="IXF54_001015"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1404293.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IXF54_001016"
     CDS             12635..13393
                     /locus_tag="IXF54_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1404294.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IXF54_001017"
     CDS             13894..15093
                     /locus_tag="IXF54_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404295.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IXF54_001018"
     CDS             15115..15327
                     /locus_tag="IXF54_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1404296.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IXF54_001019"
     CDS             15402..15878
                     /locus_tag="IXF54_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ1404297.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IXF54_001020"
     CDS             15875..17683
                     /locus_tag="IXF54_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1404298.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IXF54_001021"
     CDS             17760..17975
                     /locus_tag="IXF54_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ1404299.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IXF54_001022"
     CDS             18067..18930
                     /locus_tag="IXF54_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404300.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IXF54_001023"
     CDS             18961..19503
                     /locus_tag="IXF54_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ1404301.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IXF54_001024"
     CDS             19517..19939
                     /locus_tag="IXF54_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1404302.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IXF54_001025"
     CDS             19869..22268
                     /locus_tag="IXF54_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1404303.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IXF54_001026"
     CDS             22300..24291
                     /locus_tag="IXF54_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ1404304.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IXF54_001027"
     CDS             24314..24565
                     /locus_tag="IXF54_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ1404305.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IXF54_001028"
     CDS             24555..25784
                     /locus_tag="IXF54_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ1404306.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IXF54_001029"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IXF54_001029"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1404307.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IXF54_001030"
     CDS             28011..31931
                     /locus_tag="IXF54_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ1404308.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IXF54_001031"
     CDS             31932..32876
                     /locus_tag="IXF54_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404309.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IXF54_001032"
     CDS             32880..33083
                     /locus_tag="IXF54_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404310.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IXF54_001033"
     CDS             complement(33383..33619)
                     /locus_tag="IXF54_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404311.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IXF54_001034"
     CDS             complement(33802..34173)
                     /locus_tag="IXF54_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404312.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IXF54_001035"
     CDS             complement(34170..35498)
                     /locus_tag="IXF54_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1404313.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IXF54_001036"
     CDS             complement(35459..35788)
                     /locus_tag="IXF54_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1404314.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IXF54_001037"
     CDS             complement(36046..36417)
                     /locus_tag="IXF54_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1404315.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IXF54_001038"
     CDS             36562..36747
                     /locus_tag="IXF54_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1404316.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37690..38349
                     /locus_tag="IXF54_001039"
     CDS             37690..38349
                     /locus_tag="IXF54_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ1404317.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IXF54_001040"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IXF54_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1404318.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40672
                     /gene="vanY"
                     /locus_tag="IXF54_001041"
     CDS             39866..40672
                     /gene="vanY"
                     /locus_tag="IXF54_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1404319.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASALEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40690..41501
                     /gene="vanW"
                     /locus_tag="IXF54_001042"
                     /pseudo
     CDS             40690..41501
                     /gene="vanW"
                     /locus_tag="IXF54_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11617 a         9543 c        10341 g        10000 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg gtggagactg gcttccgcat tggaggcaac
    40021 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    40081 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    40141 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    40201 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    40261 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    40321 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    40381 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    40441 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    40501 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    40561 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    40621 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    40681 tgttatgtta tggacagaaa aagattgaca cagcgtttcc cgttcctgct tccaatgaga
    40741 cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg tcgcgctatg
    40801 cgcagacgat aggggaaaaa tcgctttccc atttgctctt tgaaacggat tgcgcattat
    40861 acaaccacaa taccggattt gacatgatat accaggaaaa caaggtgttc aacttaaagc
    40921 tggcagcaaa gaccttaaac ggcttattga taagaccggg ggaaaccttt tctttctggt
    40981 ggctggtacg ccatgcggac aaagataccc cctataaaga cggccttacg gtgaccaatg
    41041 gtaaactcac caccatgtcg ggcggcggta tgtgccagat gagcaattta ctattttgga
    41101 tgttcctgca tacgccattg acaattatcc agcgcagagg tcacgaagta aaggagtttc
    41161 cagagccaaa cagtgacgag attaaagggg tggatgcaac catttcagag ggctggattg
    41221 atttaaaagt gcgaaacgat accgactgca cctaccaaat atgagtgacc ttagatgatg
    41281 agaaaatcat cggtcaggtg tccgccgaca aagagcccca agcattatac aaaattacaa
    41341 atggcagtat ccagtatgtc cgtgaaagtg gcgggattta tgaatatgcc caggttaaac
    41401 ggatgcaagt tgccttaggt accggggaaa taatagattg caagctgctt tatacaaaca
    41461 aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat t
//
LOCUS       DACERL010000020        40672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.021, whole genome shotgun sequence.
ACCESSION   DACERL010000020 DACERL010000000
VERSION     DACERL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40672
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.021"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(7..963)
                     /locus_tag="IXF54_001043"
     CDS             complement(7..963)
                     /locus_tag="IXF54_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1404320.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IXF54_001044"
     CDS             1137..1697
                     /locus_tag="IXF54_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404321.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IXF54_001045"
     CDS             1690..2514
                     /locus_tag="IXF54_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1404322.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IXF54_001046"
     CDS             2524..3141
                     /locus_tag="IXF54_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1404323.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="IXF54_001047"
     CDS             3159..3842
                     /locus_tag="IXF54_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1404324.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5808
                     /locus_tag="IXF54_001048"
     CDS             4525..5808
                     /locus_tag="IXF54_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1404325.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5824..6711
                     /locus_tag="IXF54_001049"
     CDS             5824..6711
                     /locus_tag="IXF54_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404326.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6783..7559
                     /locus_tag="IXF54_001050"
     CDS             6783..7559
                     /locus_tag="IXF54_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404327.1"
                     /translation="MIYPVLWMIAGSFKTDQEIMSGSLNLIPETFQWENYAKGWAGFA
                     GISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDFKGKRFWFVVMLVTMMIPAQV
                     ILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQFMVSIPKELDESAIIDGCSK
                     YSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYLNKPRSYTVSIAIKLFADAGS
                     STTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGLKG"
     gene            7562..8185
                     /locus_tag="IXF54_001051"
     CDS             7562..8185
                     /locus_tag="IXF54_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404328.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8182..9474
                     /locus_tag="IXF54_001052"
     CDS             8182..9474
                     /locus_tag="IXF54_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1404329.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9833..9930
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10046..10627
                     /locus_tag="IXF54_001053"
     CDS             10046..10627
                     /locus_tag="IXF54_001053"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1404330.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10630..11937
                     /locus_tag="IXF54_001054"
     CDS             10630..11937
                     /locus_tag="IXF54_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1404331.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12353..12841
                     /gene="purE"
                     /locus_tag="IXF54_001055"
     CDS             12353..12841
                     /gene="purE"
                     /locus_tag="IXF54_001055"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1404332.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12825..13958
                     /gene="purK"
                     /locus_tag="IXF54_001056"
     CDS             12825..13958
                     /gene="purK"
                     /locus_tag="IXF54_001056"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1404333.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13971..15266
                     /gene="purB"
                     /locus_tag="IXF54_001057"
     CDS             13971..15266
                     /gene="purB"
                     /locus_tag="IXF54_001057"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1404334.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15446..15823)
                     /locus_tag="IXF54_001058"
     CDS             complement(15446..15823)
                     /locus_tag="IXF54_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404335.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16079..16807
                     /locus_tag="IXF54_001059"
     CDS             16079..16807
                     /locus_tag="IXF54_001059"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1404336.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16807..17061
                     /gene="purS"
                     /locus_tag="IXF54_001060"
     CDS             16807..17061
                     /gene="purS"
                     /locus_tag="IXF54_001060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1404337.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17063..17734
                     /gene="purQ"
                     /locus_tag="IXF54_001061"
     CDS             17063..17734
                     /gene="purQ"
                     /locus_tag="IXF54_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1404338.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17735..19957
                     /gene="purL"
                     /locus_tag="IXF54_001062"
     CDS             17735..19957
                     /gene="purL"
                     /locus_tag="IXF54_001062"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1404339.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19942..21381
                     /locus_tag="IXF54_001063"
     CDS             19942..21381
                     /locus_tag="IXF54_001063"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1404340.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21413..22456
                     /locus_tag="IXF54_001064"
     CDS             21413..22456
                     /locus_tag="IXF54_001064"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1404341.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22453..23031
                     /gene="purN"
                     /locus_tag="IXF54_001065"
     CDS             22453..23031
                     /gene="purN"
                     /locus_tag="IXF54_001065"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1404342.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23028..24563
                     /gene="purH"
                     /locus_tag="IXF54_001066"
     CDS             23028..24563
                     /gene="purH"
                     /locus_tag="IXF54_001066"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1404343.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24579..25853
                     /gene="purD"
                     /locus_tag="IXF54_001067"
     CDS             24579..25853
                     /gene="purD"
                     /locus_tag="IXF54_001067"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1404344.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25975..26889
                     /locus_tag="IXF54_001068"
     CDS             25975..26889
                     /locus_tag="IXF54_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1404345.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27022..28587)
                     /locus_tag="IXF54_001069"
     CDS             complement(27022..28587)
                     /locus_tag="IXF54_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1404346.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28580..30031)
                     /locus_tag="IXF54_001070"
     CDS             complement(28580..30031)
                     /locus_tag="IXF54_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1404347.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30045..31061)
                     /locus_tag="IXF54_001071"
     CDS             complement(30045..31061)
                     /locus_tag="IXF54_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404348.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31281..32369
                     /locus_tag="IXF54_001072"
     CDS             31281..32369
                     /locus_tag="IXF54_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404349.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32423..33949
                     /locus_tag="IXF54_001073"
     CDS             32423..33949
                     /locus_tag="IXF54_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404350.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33951..35156
                     /locus_tag="IXF54_001074"
     CDS             33951..35156
                     /locus_tag="IXF54_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404351.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35413..36390
                     /gene="guaC"
                     /locus_tag="IXF54_001075"
     CDS             35413..36390
                     /gene="guaC"
                     /locus_tag="IXF54_001075"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1404352.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36654..38078
                     /locus_tag="IXF54_001076"
     CDS             36654..38078
                     /locus_tag="IXF54_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1404353.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38539..39648
                     /gene="pdhA"
                     /locus_tag="IXF54_001077"
     CDS             38539..39648
                     /gene="pdhA"
                     /locus_tag="IXF54_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1404354.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39651..40628
                     /locus_tag="IXF54_001078"
     CDS             39651..40628
                     /locus_tag="IXF54_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1404355.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        12982 a         6937 c         9112 g        11641 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca gcaggaggaa
     5821 ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt cggttacgta
     5881 tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat gatcttgtca
     5941 ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat cggcttggat
     6001 aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt cacttttctc
     6061 tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc ttatctttta
     6121 acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc tagtttgatc
     6181 ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa cgggttagtg
     6241 aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga tcaaaaactg
     6301 gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat gatcatcttt
     6361 gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat cgatggagcg
     6421 aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat cattttgtat
     6481 aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt tatcattacc
     6541 caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa ccaagctttt
     6601 tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt gattatggga
     6661 gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg aatgagagga
     6721 ggaagaaatg aataataccg aaatggtttc aatacggttt tattttgcta ttaggtctag
     6781 tcatgattta tccggtctta tggatgatcg caggttcttt taaaacggat caagagatta
     6841 tgagtggttc gctgaatttg attccagaga cttttcaatg ggaaaattac gcaaaaggat
     6901 gggcggggtt tgctggaatt tcatttttta ctttctttag gaattcgttt gtcatctctg
     6961 cagtttcgac gatagggaca gttctgagct cgacttgtat tgcttacgca ttatcgagaa
     7021 tcgatttcaa aggaaaaaga ttctggttcg tcgtcatgtt agtaacgatg atgattcctg
     7081 cacaagtgat tttgattccg cagtttatta tttacaatcg gctgaatttg gttgggacgt
     7141 atgtgccgct gattttacca catttttttg gacaagcatt tttcatttac cagatcatgc
     7201 aattcatggt cagcatcccc aaagagttag atgaatcagc gattattgac ggatgcagca
     7261 agtatagtgt ctttaccaaa attatttttc ctttgctgaa accttcgatc atcacgacga
     7321 tcatcatcca gttttattgg aaatgggacg acttcatggg accattgatt tatttaaaca
     7381 agccgagatc ttacacggta tcgattgcca tcaaattatt tgcagacgct ggatctagta
     7441 cgacttacgc gagtatgttt gcgatgtcta cgttgtcgct gctaccggtt tttctgattt
     7501 tcttattctt caataaatat cttgtccagg ggatcagtac aagcggtctg aagggctgaa
     7561 tatgaaaaag ccaatagaag agatgtttct ttacaagctc aactactatg tctgtcggat
     7621 ttttttaacc agtcttatca gtaatgctct tttcgcttta gttctaggat gtttgttcac
     7681 tttaggatta gtgctgtctg ctcgtgttgt ctatctgatt tattttttga tgatgtatcc
     7741 ttcttattat ctatctgtcc attacttggc agaaaaaaag gtatgttgga ggaaaggaaa
     7801 aatcaaacgg atgtgtttca tcattacgtt gtttcagctg tttgtcatta tttttgcagt
     7861 tgattggtat ttattagtgg gaaagattgg agcttggtat ctgattcctt ttctattgtg
     7921 tctgttgctt tttgtatccc atagtttttt tagtacgcta attgatatct gcaaagaaga
     7981 taaagcagat agtcttgttt atttgatcaa aaggaatatc tggctgttgt caaaaaacgt
     8041 caaggggttt ctgtggtatt tatgcctttt gtatggactg ttctggtgta tacagcatga
     8101 ttttttactc actgcattac tgtatccagg gctgccattt gtcacagtat tttgcttaaa
     8161 agaaaaggag aatgacttta aatgaagaaa aaatatgtac aagtcggact tggtgggaga
     8221 gcgcgttttt tttatgaggc aattgcctct acgtatcatc aaacttgtga actagtcggt
     8281 ttttgtgatg tatctcaaac aaggatggat tatgcaaatc ggatacttgt gaacaagtat
     8341 ggacagaaaa aagtaaagac atgtatgcca tcacaatttg agcaactact ggatgacgta
     8401 cggccggctg aagtcattat tacgtcagta gatcgaacgc atcatgatta tatcattcgt
     8461 gcaatgaaaa aaggatatga tgttatttgc gaaaaacctc tgactatcga tgaggaaaaa
     8521 gctcaagaaa taataacggt ccaaaaacag acgggaagaa gcctgcgagt cacttttaat
     8581 tatcgttatg caccgtatca cacgaagata aaggaactga tagaaagtga tgtgattggt
     8641 gatgtgtttt ctatccactt cgaatggctg ttaaatacgc aacacggggc agattatttc
     8701 agaagatggc atcgaaataa gcacaatagc ggcgggcttc ttgttcataa atctacccat
     8761 cattttgatc tagtgaattt ttggttgaac agccgtcccc aaaaggtatt tgcattcggt
     8821 ggattaaact tttatgggat gcataatgcg gagaaacgcg gagtagaaat attttacagt
     8881 cgatgctatg gaagcgaact agctaaagct gatccatttg ctatagacct gtcagccaat
     8941 gaggaattgc gagaaatgta tttagaagca gaaaaagaca gtggatatat tcgtgaccgc
     9001 agtgtatttt ccgatgatat ctctattgaa gatacactag gcgtaacggt caaatatgaa
     9061 actggtgcga ttttgacgta ttcgctaaac gcgtatatgc cgtgggaagg attcaatgtt
     9121 tctttcaatg gcagcaaagg acggatcgaa atgaaagtga tcgaaaagtc ttacatcaat
     9181 gcaggtggag ataaaaagga tgaaggagca gccgttcttc agtccatcca tgtctatccg
     9241 atgttcggtg agccatatga ggtaatcgtc gaacaagaag cgggcggaca tggcggagga
     9301 gatccgcgtt tattagatga tctatttggt gaagaaaaag aagaagatcc gtggaatcgg
     9361 gcagcaactc acgtagatgg tattttgtcg attttgacag gaatcgcagc aaatcattct
     9421 attgcttcag gaaaagcagt agtgattgac gagcttgtca gcttaaaaga ataagaatag
     9481 atttacggaa attacttccc gtagtttata aaatcttgcc ttattgccga taggctgtct
     9541 tttgaacact atttattcgg aaaaaggggc tatgacatgc tttttgtcat gccttctttt
     9601 tatgtgggct gcttttttag ataggagaaa ataaaaataa ctatttaaac ccgaacaaaa
     9661 aggaataatg cattaaaaat cattagattt tacaaaaaaa taagtaaacc acgttgtcag
     9721 tttcagaaag tagtgttaga ctgttttcaa tatttgaaac aacatttttt attcaaatat
     9781 ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg aatttacact attattagtt
     9841 ttacatacct acttatatat cgtcataata tggatgacag tttctagcca gtaccgtaaa
     9901 tgctggacta taagtaaaag attggctatt tcacccttct tttcttatga taaagtggag
     9961 ggtgtttttc tttaacagaa acatagtagg tttttgcaca aaagcggtta tttcggtatt
    10021 aataaatgat aaaggagcaa ataaagtgaa agaattagtt gaacgtattc aaaaagacgg
    10081 acgtatatta ggcgagggcg tattaaaagt cgacagcttc gtcactcacc aagtagaccc
    10141 tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac aaggaatcac
    10201 gaaagttgtg accattgaag cttctgggat cgcaccagct ttatttgctg caagagaatt
    10261 agatgttcca atgatcttcg ctagaaaagc aaaaagtttg acgatggatg aagaattatt
    10321 gacagcttct gtttattctt ttacgaaaca agtgacaagt acgatttcta tttctcgtaa
    10381 atttttatct tcagaagaca aagtattgat tatcgacgat ttcttggcaa acggacaagc
    10441 ggcaaaagga ttgatcgaat tatgccagca agctggagcg caagtagaag gaatcgggat
    10501 cgtcatcgaa aagtctttcc aagatggacg tcagttactt gaagaaatgg gattgagagt
    10561 cgtttctctt gcgcgtattg cttccttgac gaatggacaa gttgagtttc ttgaggagga
    10621 cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg cggttttagg gctgcagcat
    10681 ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac aggactaggc
    10741 tttaatcaag aacagatgac ttatttgatt tcaattgata tctttatgtg tggattagcg
    10801 acattattgc aattgacagt taaccgattt ttcggaattg gcttgcctgt cgttttagga
    10861 tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa ccaatgaagg agtaggagct
    10921 atctacggat ctattatcgc ttctgggatc tttgttgtgt tagtttctgg tgtcttttct
    10981 aaaattaaaa aactattccc tcctatagta acaggaacgg ttatcaccgt tattggtttg
    11041 actttgattc ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc agctgatttt
    11101 ggtgacaaaa caaatctgct acttgcattt gtgacgattc tcctaattgt aggcgtgcaa
    11161 atgctggcaa aagggttcat tcgttcaatc gcggtattga ttggtttagt cggaggaagc
    11221 atcttagctg ctttcatggg aatgattgat ctgagcgcga ttggagatgc gccgttgttc
    11281 catgttccac aaccctttta ttttggtaaa ccaactttcg acgtatggtc gattttacta
    11341 atgatcatca tttccatggt cagcatggta gaatcaacag gtgtatattt tgcacttggt
    11401 gacattactg ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg tgcagaagga
    11461 cttgccgtta tcttaggcgg tattttcaat acattccctt atactggctt ttctcaaaat
    11521 gtcggattgg ttcaactttc gggaatcaaa acaagaaaac ccatctattt ctctgcattc
    11581 ttcttgattg ttttaggatt attccctaaa atcggtgcgt tagcacaaat catccctgag
    11641 ccggtacttg gtggaggaat gttagtcatg ttcgggatgg tggcagtcca aggaatccgt
    11701 atgctgttgg aagttgattt cagcaatgat aaaaatctat tgattgttgc tgtttctgtt
    11761 ggatttgggc tgggtttcaa tgttacgccg acattattca gtcaaatgcc gcaaacattg
    11821 caaatgttca ctggaaatgg catcgtaatg agtagcatta cggctatcat attgaatttg
    11881 atctttaatg gattgaaaaa agatgaagaa atattggaag aagccttcaa gaagtaatag
    11941 agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt gttagagaaa caaacggaaa
    12001 aatagttcct cgtattttgc cgtttgtttg agctaatacc gagggtcagc tgattgaaca
    12061 ccgttgaagg aagaggtcgt gagcctgccg ttcatctccg agtcacaagg aacaatttca
    12121 aaaaaaagct tctcatgttt gttgaatatt gttccttgtg accgaggagt tggcaggaga
    12181 acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct tttttttatc
    12241 tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat
    12301 caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc
    12361 aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca
    12421 aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc
    12481 agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc
    12541 aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt
    12601 tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt
    12661 ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa
    12721 tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt
    12781 ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta
    12841 aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga
    12901 tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt
    12961 gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc
    13021 tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag
    13081 aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga
    13141 ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc
    13201 cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc
    13261 cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa
    13321 gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag
    13381 catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt
    13441 tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg
    13501 ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg
    13561 cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg
    13621 ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag
    13681 cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag
    13741 aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg
    13801 aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac
    13861 aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa
    13921 cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac
    13981 gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc
    14041 tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag
    14101 atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa
    14161 aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag
    14221 aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct
    14281 atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca
    14341 ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc
    14401 atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga
    14461 acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag
    14521 tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga
    14581 tccgtccaca agaaatttct actcaagttc tccctcggga tctacatgct gaatatttct
    14641 cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc
    14701 aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg
    14761 ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg
    14821 tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt
    14881 ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt
    14941 taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga
    15001 atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata
    15061 agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg
    15121 acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat
    15181 ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga
    15241 tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga
    15301 atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat
    15361 tatattttct tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc
    15421 tctttacata caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat
    15481 gctggttatg gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat
    15541 tgaataagat ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga
    15601 ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac
    15661 agcgcttggt tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata
    15721 aataaacgtc tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta
    15781 attctgataa aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg
    15841 aacccattat ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac
    15901 gcttgttgat ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat
    15961 gccttaaatg tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga
    16021 aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat
    16081 ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga
    16141 gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga
    16201 ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct
    16261 gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc
    16321 aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt
    16381 ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta
    16441 ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca
    16501 acttgcaaag ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat
    16561 tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg
    16621 ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg
    16681 ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt
    16741 gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg
    16801 agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa
    16861 ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga
    16921 ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt
    16981 gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc
    17041 aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg
    17101 tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg
    17161 gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta
    17221 tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat
    17281 tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac
    17341 agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa
    17401 aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga
    17461 aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact
    17521 tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag
    17581 cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca
    17641 tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc
    17701 cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc
    17761 tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg
    17821 aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc
    17881 tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc
    17941 tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg
    18001 tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc
    18061 ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc
    18121 tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa
    18181 atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc
    18241 gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc
    18301 aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg
    18361 taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt
    18421 tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga
    18481 tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga
    18541 ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat
    18601 ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac
    18661 tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt
    18721 taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt
    18781 cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt
    18841 agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa
    18901 agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga
    18961 acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat
    19021 ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc
    19081 tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc
    19141 gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag
    19201 aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc
    19261 tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc
    19321 gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga
    19381 tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa
    19441 acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac
    19501 gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt
    19561 gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat
    19621 ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact
    19681 ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc
    19741 tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac
    19801 acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc
    19861 gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa
    19921 gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg
    19981 atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt
    20041 cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag
    20101 gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag
    20161 ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg
    20221 gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat
    20281 ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc
    20341 cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat
    20401 tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta
    20461 atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc
    20521 ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt
    20581 ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga
    20641 atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc
    20701 tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat
    20761 atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct
    20821 aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc
    20881 ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga
    20941 gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg
    21001 attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg
    21061 aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca
    21121 tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt
    21181 gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg
    21241 atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc
    21301 aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa
    21361 gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa
    21421 tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa
    21481 gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg
    21541 ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg
    21601 cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga
    21661 ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt
    21721 agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt
    21781 agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc
    21841 tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa
    21901 atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc
    21961 tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca
    22021 gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga
    22081 gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg
    22141 agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc
    22201 tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca
    22261 aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat
    22321 ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg
    22381 cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat
    22441 aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg
    22501 ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat
    22561 cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt
    22621 tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa
    22681 gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg
    22741 ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct
    22801 ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt
    22861 cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt
    22921 gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc
    22981 tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat
    23041 tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag
    23101 gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa
    23161 cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac
    23221 ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg
    23281 caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca
    23341 aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg
    23401 gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg
    23461 atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt
    23521 ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga
    23581 tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt
    23641 atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc
    23701 agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac
    23761 tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg
    23821 agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga
    23881 ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca
    23941 tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag
    24001 aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa
    24061 acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg
    24121 tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag
    24181 agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagtgtttg
    24241 cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga
    24301 cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac
    24361 aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg
    24421 attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca
    24481 tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg
    24541 atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg
    24601 caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg
    24661 tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca
    24721 gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta
    24781 tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat
    24841 tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta
    24901 aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg
    24961 aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct
    25021 tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag
    25081 aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt
    25141 ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga
    25201 tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg
    25261 gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg
    25321 aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg
    25381 ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc
    25441 gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag
    25501 taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca
    25561 atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc
    25621 taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg
    25681 gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca
    25741 aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt
    25801 atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct
    25861 gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt
    25921 ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa
    25981 aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt
    26041 atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat
    26101 ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct
    26161 ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt
    26221 ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg
    26281 acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta
    26341 gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat
    26401 tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa
    26461 ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg
    26521 attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat
    26581 tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa
    26641 ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca
    26701 gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc
    26761 aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct
    26821 tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt
    26881 ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac
    26941 ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta
    27001 tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata
    27061 ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt
    27121 ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt
    27181 tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga
    27241 aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga
    27301 catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg
    27361 atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac
    27421 tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca
    27481 atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt
    27541 caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg
    27601 ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa
    27661 gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat
    27721 ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg
    27781 taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc
    27841 cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc
    27901 ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac
    27961 ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa
    28021 gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg
    28081 attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc
    28141 gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt
    28201 ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt
    28261 aatcggttta agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa
    28321 atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt
    28381 tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg
    28441 taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat
    28501 tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc
    28561 gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag
    28621 ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt
    28681 gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac
    28741 tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag
    28801 ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgtgg
    28861 taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa
    28921 tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga
    28981 tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat
    29041 ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa
    29101 agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag
    29161 aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac
    29221 gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta
    29281 gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca
    29341 gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat
    29401 tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa
    29461 tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg
    29521 tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca
    29581 gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt
    29641 ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta
    29701 gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa
    29761 tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc
    29821 cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat
    29881 ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca
    29941 gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa
    30001 aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt
    30061 gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt
    30121 aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag
    30181 ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc
    30241 atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt
    30301 tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg
    30361 atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga
    30421 ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca
    30481 tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg
    30541 gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa
    30601 tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg
    30661 ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg
    30721 acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa
    30781 ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc
    30841 cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca
    30901 gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa
    30961 ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa
    31021 gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg
    31081 atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga
    31141 aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa
    31201 gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca
    31261 attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta
    31321 ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta
    31381 cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga
    31441 acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag
    31501 ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac
    31561 ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag
    31621 cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt
    31681 acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact
    31741 taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg
    31801 acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca
    31861 acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct
    31921 gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc
    31981 aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat
    32041 taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg
    32101 cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag
    32161 acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag
    32221 aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt
    32281 tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt
    32341 attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta
    32401 agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa
    32461 aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat
    32521 ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg
    32581 cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt
    32641 taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact
    32701 aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg
    32761 cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt
    32821 gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga
    32881 aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc
    32941 attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg
    33001 tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat
    33061 caccatttta cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga
    33121 gcatattatt cgcggaatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc
    33181 ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca
    33241 cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat
    33301 agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta
    33361 tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt
    33421 tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact
    33481 gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca
    33541 aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat
    33601 gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca
    33661 aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc
    33721 aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc
    33781 agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg
    33841 tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc
    33901 agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg
    33961 caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc
    34021 tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga
    34081 tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc
    34141 atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt
    34201 cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc
    34261 aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat
    34321 ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat
    34381 tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc
    34441 cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc
    34501 ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat
    34561 ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct
    34621 tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt
    34681 atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt
    34741 tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg
    34801 ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag
    34861 tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag
    34921 caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt
    34981 ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga
    35041 acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga
    35101 tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga
    35161 acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta
    35221 aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat
    35281 tcgtataaat tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg
    35341 cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag
    35401 gaggataatg taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt
    35461 atcgtaaata gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa
    35521 atgccagtag tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta
    35581 gcggaaaatg gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt
    35641 atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag
    35701 gaatattcct ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc
    35761 gacatcgcgc atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat
    35821 ttgcctgaaa cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg
    35881 gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca
    35941 aaaatcaaga caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg
    36001 aaagcagctc gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca
    36061 aaatctgtcc gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag
    36121 gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct
    36181 gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa
    36241 cacaaaggat ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt
    36301 tcttatgctg gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa
    36361 aattcgattt ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa
    36421 aaagttgtcc tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt
    36481 gtttggctgg aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg
    36541 actgtgataa gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc
    36601 tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg
    36661 aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag
    36721 aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc
    36781 aactttttgg tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg
    36841 gggtactaac gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat
    36901 tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact
    36961 ggtatgagca aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat
    37021 acgctagcga gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg
    37081 aagaaatctt agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca
    37141 aaatcaaacg gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg
    37201 tgactggtgg cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag
    37261 aggggatagc tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag
    37321 gaacgcgttt atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag
    37381 aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta
    37441 caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc
    37501 ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag
    37561 tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg
    37621 ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac
    37681 tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg
    37741 ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag
    37801 aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa
    37861 ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag
    37921 cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata
    37981 taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt
    38041 caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa
    38101 agaatataac agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac
    38161 gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa
    38221 ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt
    38281 aagtttgatt agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga
    38341 ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt
    38401 tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa
    38461 tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga
    38521 gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta
    38581 gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt
    38641 aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg
    38701 gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc
    38761 ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat
    38821 aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta
    38881 cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa
    38941 gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt
    39001 gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat
    39061 ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca
    39121 aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa
    39181 actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc
    39241 gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc
    39301 agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta
    39361 tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa
    39421 gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa
    39481 gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac
    39541 aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa
    39601 tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa
    39661 aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg
    39721 tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat
    39781 tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg
    39841 caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct
    39901 ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc
    39961 gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc
    40021 atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc
    40081 gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa
    40141 gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag
    40201 tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag
    40261 atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc
    40321 ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg
    40381 tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac
    40441 aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt
    40501 cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag
    40561 cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg
    40621 aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa tt
//
LOCUS       DACERL010000021        39005 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.022, whole genome shotgun sequence.
ACCESSION   DACERL010000021 DACERL010000000
VERSION     DACERL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39005
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.022"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(175..732)
                     /gene="frr"
                     /locus_tag="IXF54_001079"
     CDS             complement(175..732)
                     /gene="frr"
                     /locus_tag="IXF54_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1404356.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(735..1457)
                     /locus_tag="IXF54_001080"
     CDS             complement(735..1457)
                     /locus_tag="IXF54_001080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1404357.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1593..2474)
                     /locus_tag="IXF54_001081"
     CDS             complement(1593..2474)
                     /locus_tag="IXF54_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1404358.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="IXF54_001082"
     CDS             complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="IXF54_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1404359.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3714..4193
                     /locus_tag="IXF54_001083"
     CDS             3714..4193
                     /locus_tag="IXF54_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1404360.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4410..5501
                     /gene="phnW"
                     /locus_tag="IXF54_001084"
     CDS             4410..5501
                     /gene="phnW"
                     /locus_tag="IXF54_001084"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1404361.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5494..5622
                     /locus_tag="IXF54_001085"
     CDS             5494..5622
                     /locus_tag="IXF54_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1404362.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5626..6171
                     /locus_tag="IXF54_001086"
     CDS             5626..6171
                     /locus_tag="IXF54_001086"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1404363.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6625..8316)
                     /locus_tag="IXF54_001087"
     CDS             complement(6625..8316)
                     /locus_tag="IXF54_001087"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1404364.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8736..9683)
                     /gene="arcC"
                     /locus_tag="IXF54_001088"
     CDS             complement(8736..9683)
                     /gene="arcC"
                     /locus_tag="IXF54_001088"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1404365.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9798..10817)
                     /gene="argF"
                     /locus_tag="IXF54_001089"
     CDS             complement(9798..10817)
                     /gene="argF"
                     /locus_tag="IXF54_001089"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1404366.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10908..12137)
                     /gene="arcA"
                     /locus_tag="IXF54_001090"
     CDS             complement(10908..12137)
                     /gene="arcA"
                     /locus_tag="IXF54_001090"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1404367.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12598..13299
                     /locus_tag="IXF54_001091"
     CDS             12598..13299
                     /locus_tag="IXF54_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1404368.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13908..14924)
                     /gene="tsaD"
                     /locus_tag="IXF54_001092"
     CDS             complement(13908..14924)
                     /gene="tsaD"
                     /locus_tag="IXF54_001092"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1404369.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14921..15385)
                     /gene="rimI"
                     /locus_tag="IXF54_001093"
     CDS             complement(14921..15385)
                     /gene="rimI"
                     /locus_tag="IXF54_001093"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1404370.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15392..15934)
                     /gene="rimI"
                     /locus_tag="IXF54_001094"
     CDS             complement(15392..15934)
                     /gene="rimI"
                     /locus_tag="IXF54_001094"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1404371.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15918..16742)
                     /gene="tsaB"
                     /locus_tag="IXF54_001095"
     CDS             complement(15918..16742)
                     /gene="tsaB"
                     /locus_tag="IXF54_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1404372.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16831..17811)
                     /locus_tag="IXF54_001096"
     CDS             complement(16831..17811)
                     /locus_tag="IXF54_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1404373.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17835..19319)
                     /gene="galT"
                     /locus_tag="IXF54_001097"
     CDS             complement(17835..19319)
                     /gene="galT"
                     /locus_tag="IXF54_001097"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1404374.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19331..20320)
                     /gene="galE"
                     /locus_tag="IXF54_001098"
     CDS             complement(19331..20320)
                     /gene="galE"
                     /locus_tag="IXF54_001098"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1404375.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20568..20735
                     /locus_tag="IXF54_001099"
     CDS             20568..20735
                     /locus_tag="IXF54_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1404376.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20797..22608)
                     /locus_tag="IXF54_001100"
     CDS             complement(20797..22608)
                     /locus_tag="IXF54_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1404377.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22605..22970)
                     /locus_tag="IXF54_001101"
     CDS             complement(22605..22970)
                     /locus_tag="IXF54_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1404378.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23133..23528)
                     /locus_tag="IXF54_001102"
     CDS             complement(23133..23528)
                     /locus_tag="IXF54_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1404379.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23546..24508)
                     /locus_tag="IXF54_001103"
     CDS             complement(23546..24508)
                     /locus_tag="IXF54_001103"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1404380.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24508..24720)
                     /locus_tag="IXF54_001104"
     CDS             complement(24508..24720)
                     /locus_tag="IXF54_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1404381.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24741..25439)
                     /locus_tag="IXF54_001105"
     CDS             complement(24741..25439)
                     /locus_tag="IXF54_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1404382.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25459..26001)
                     /locus_tag="IXF54_001106"
     CDS             complement(25459..26001)
                     /locus_tag="IXF54_001106"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1404383.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26133..27137)
                     /locus_tag="IXF54_001107"
     CDS             complement(26133..27137)
                     /locus_tag="IXF54_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1404384.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27134..28123)
                     /locus_tag="IXF54_001108"
     CDS             complement(27134..28123)
                     /locus_tag="IXF54_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1404385.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28120..28926)
                     /locus_tag="IXF54_001109"
     CDS             complement(28120..28926)
                     /locus_tag="IXF54_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404386.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            29092..30048
                     /locus_tag="IXF54_001110"
     CDS             29092..30048
                     /locus_tag="IXF54_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404387.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(30125..30643)
                     /locus_tag="IXF54_001111"
     CDS             complement(30125..30643)
                     /locus_tag="IXF54_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1404388.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30731..30880)
                     /gene="rpmG"
                     /locus_tag="IXF54_001112"
     CDS             complement(30731..30880)
                     /gene="rpmG"
                     /locus_tag="IXF54_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1404389.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            31108..31554
                     /locus_tag="IXF54_001113"
     CDS             31108..31554
                     /locus_tag="IXF54_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1404390.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31749..33644)
                     /gene="asnB"
                     /locus_tag="IXF54_001114"
     CDS             complement(31749..33644)
                     /gene="asnB"
                     /locus_tag="IXF54_001114"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1404391.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33969..34943)
                     /locus_tag="IXF54_001115"
     CDS             complement(33969..34943)
                     /locus_tag="IXF54_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1404392.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35277..35364)
                     /locus_tag="IXF54_001116"
     tRNA            complement(35277..35364)
                     /locus_tag="IXF54_001116"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35330..35332),aa:Ser,seq:cga)
     gene            complement(35509..36075)
                     /locus_tag="IXF54_001117"
     CDS             complement(35509..36075)
                     /locus_tag="IXF54_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1404393.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36331..37662
                     /locus_tag="IXF54_001118"
     CDS             36331..37662
                     /locus_tag="IXF54_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1404394.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37628..37978
                     /locus_tag="IXF54_001119"
     CDS             37628..37978
                     /locus_tag="IXF54_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404395.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(38070..38489)
                     /locus_tag="IXF54_001120"
                     /pseudo
     CDS             complement(38070..38489)
                     /locus_tag="IXF54_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(38490..38834)
                     /locus_tag="IXF54_001121"
     CDS             complement(38490..38834)
                     /locus_tag="IXF54_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1404396.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT        11319 a         8090 c         6765 g        12831 t
ORIGIN      
        1 aaagattccc actatcttaa gtattatagc aatatctaac tgtttttcct atgaaatcat
       61 tgttttttta tttaaaaact gaataatttt ttcttttttt aatttttaaa taaaagaaat
      121 ataaataaaa acagaggaag cggaatgatc cgcccctctg tttttatgat tttattaaac
      181 ttccaataat tctttttctt tatcagcagc gatcttatct acttctttga tgctgttatc
      241 tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt
      301 tttttgttgt tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt
      361 tgcattttct gcttcttttt tcacatcttt agctaattct tttctgcgtt cttctgtcag
      421 ttgcgggata actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact
      481 tgccataata gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa
      541 aacacgtgct tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta
      601 gtagttcaca gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc
      661 aagttcacgt tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat
      721 tgcatctgcc atttttattt cccccttaca gttgttccga tattttcgcc taagattgca
      781 cggcgaatat ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg
      841 cttagtgaac ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga
      901 gtcagttctt caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg
      961 tctacattgt ttttagccat caggataaca tctgcgccga tttctgccgc gcgtaatgct
     1021 gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt
     1081 cctttttcta aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt
     1141 tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct
     1201 aaggcattca taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc
     1261 atttgtgctc cgatttgtcc gcgccagata tttccgccgc ccacaacgat tgccatttca
     1321 acgcccagct cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg
     1381 attccaaatc ctgcgtcccc agctaaagct tctccactca attttaagac tacacgctga
     1441 tatttcggtg tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta
     1501 gatgatttcg acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca
     1561 aaccgtgcgt tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct
     1621 tctacaaagt tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt
     1681 ttaacagttg cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg
     1741 aatggttggt ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt
     1801 ttttcaacga tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt
     1861 tcatgttcta attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct
     1921 gctacgtgca tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc
     1981 aatacggcaa tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt
     2041 tctacaactt caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc
     2101 aagtcagatt caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct
     2161 ggtttgtttt cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt
     2221 ttagaaacaa agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca
     2281 gcaactgaag ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt
     2341 cctttttcac gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct
     2401 tttttcgcat ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct
     2461 gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca
     2521 aaggagagtg aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg
     2581 ctgaagaatt gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt
     2641 cttctgtgaa atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat
     2701 cagccatttt tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta
     2761 ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta
     2821 atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat
     2881 ctgggattct tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt
     2941 tgttcaaacc agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg
     3001 cattgatttg tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc
     3061 cgcctaacca acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg
     3121 cttcttgtgc ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc
     3181 tcttcatgta atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt
     3241 agattccgtt tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt
     3301 ggtgaccaaa gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg
     3361 tttcctccga tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt
     3421 tcaagcacct accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca
     3481 gctattatac agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt
     3541 caatagttta cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc
     3601 tttattatcc tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat
     3661 ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca
     3721 aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag
     3781 aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc
     3841 gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg
     3901 tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc
     3961 aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata
     4021 ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta
     4081 ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg
     4141 caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc
     4201 acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt attgaataaa
     4261 cctttacact ctttacaaaa atacatcatt ttttattcaa atttttcaac accttgattg
     4321 aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt
     4381 atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaacttct ggaccattga
     4441 cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat
     4501 acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag
     4561 gaatatacac aacggtctta atgcaaggta gcagctcctt cggggtagaa tcggtattaa
     4621 gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca tatagacaac
     4681 gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac
     4741 atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccg tctatctccg
     4801 cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg
     4861 ctgctgtcac acaagaaaga gatcttttgt ttatagtgga cgcgatgtca agctttggtg
     4921 ggataccgat ttgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat
     4981 gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctggaaaaac
     5041 gaaaaaatgt tagccgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa
     5101 acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag
     5161 cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca
     5221 ataatcatgt gctacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat
     5281 atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc
     5341 atttttatca gtatttaaag caaaatggat ttgtgatcta tcccgggaaa atcagcgatc
     5401 atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga
     5461 cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg
     5521 gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt
     5581 ctagaaaaag gaacccaact gactgacaaa gaaatccgct aaccgatggg aaaattaaaa
     5641 tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt
     5701 tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat
     5761 ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa
     5821 cggttgagaa acgctgggat caatattggt tccacgaatg gatttaatgc tcctatgatg
     5881 acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac cagacttcat ggcaactttt
     5941 gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac
     6001 ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag
     6061 gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg
     6121 atttcttatg atgagtacca aaaattagag atggggaaaa agatcgttta aaacagtctg
     6181 ttgctcttag attccaacaa gccggcgctg atatttgtgt aaacaattta aaagagttaa
     6241 cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa ataagaggtt
     6301 gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa gcaaaaatag ctcctcatat
     6361 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
     6421 aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttgcaa acaaacagtg
     6481 tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca
     6541 aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa
     6601 cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg
     6661 aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg
     6721 ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt
     6781 gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc
     6841 agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc
     6901 tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt
     6961 atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc
     7021 aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg
     7081 tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc
     7141 agattggatt gcttcattta atacttcttc taataagacg atctttcctt tacgagttga
     7201 taatttttta ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt cgtcagacca
     7261 gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt
     7321 ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc
     7381 taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag
     7441 gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc
     7501 ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga
     7561 atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg
     7621 gaaccattgc cataattgaa tagcctcttt atcgccttct tccagacgtt tgaaccaagc
     7681 acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacgtataa
     7741 acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta
     7801 agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg
     7861 ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa
     7921 atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc
     7981 ttgattcccg atgttgctat ccccataatg ttctttttct ttcactactg tttgcaaaac
     8041 ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat
     8101 tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc
     8161 ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga
     8221 ttttggattt tctagtaatt gctcgacttg ttccagtgtt aaatcatctt tgacagcgtc
     8281 atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta aagtgttccc
     8341 ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga
     8401 cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc
     8461 ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca
     8521 ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca
     8581 acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag
     8641 tcgtttcata aaaaaaaaat ccagatggcc taaaggagaa gaaataccac ctggatagct
     8701 ataaatcatt tcgtaaatac gaaagactta acaaattaat cagctacgat aatcgttcct
     8761 tcttcagaag ctaaaagatt ctcgatattt tctaaagaag tgatgattgc cttagcgttt
     8821 ggttttgctt caacaaatgc aatagctgct tctactttcg gaagcatgct tcctggagcg
     8881 aattgctttt catcaatgta ttgtttcatt tctgaaacag taacagtttc taatttcttt
     8941 tgatctggtt tttggtaatt gacatacaca ttgtctactc ctgtcaatac gatcagcaaa
     9001 tccgcatcga tgatttcggc taatttttct gaagcaaaat ctttatcgat tacggcttct
     9061 cgtccttcta atccagtagc tgtttctaca acaggaatcc caccgccacc aacagagact
     9121 gtgataactc cttgatcgac cagcgtttcg atgacacgtg cttctttaat cgagatgggt
     9181 ttaggggaag cgactacttt tctccatcca cgtccagcat cttctttgaa tgtcacagtg
     9241 gagtcagcat tcatttgttc tttggcttct tcttccgtgt agaatggacc gactggttta
     9301 cttggatttt tgaatgaagg atcattttca tccacaataa cttgagtgac taaagaaacc
     9361 acatctttat cgatcccttt ttccttcagt acttcgccca tggcgttttg taaccagtaa
     9421 ccgatagatc cttctgtcat tgcaacacat gtatctaaag gcattgccgg tgttttctca
     9481 gaatcagcag cttgctgctg aatcaatagg ttaccgactt gcggaccatt tccgtgggag
     9541 atgatcaatt catctccttg ttcgataaat ttcactaaat actttgctgt ttccattaac
     9601 gcttcttgtt gggcttttgc acttgcatcc gtagacaaga tggcattgcc ccctaaagcg
     9661 acaactactt ttcgtttggc catttcattc gtcctttctt cagttctacc gaattttcta
     9721 gataacaaaa cgagccagcg ttttgtgatt ttccctcaat gctgactcgt tttttcgtga
     9781 gatgaatgtg gttatgctta aacgcgaggg ataaacaagt tacctaatgt tgctgccatg
     9841 atcgctttga ttgagtgcat acgattttct gcttggtcaa attgacgagc atatttgctg
     9901 cggaagactt catctgttac ttccatttca gtgatgccaa agcgttcttt catttgttca
     9961 ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt gcaagaagat caaacgatct
    10021 gtgttgtgtg ttttttctac catttccata ttgatttggt aaggtttcaa caatttgata
    10081 cgttcttcga atttatcttc ttcacccatt gatacccata catcagaata aaggacgtct
    10141 gctccgtcta cacctttatc aacatcgtcg gtaatcatca attgtgcacc ggatttttca
    10201 gcaaagcctt ctgccatttt gacgatttct tcttcaggtt gtaattcttt tggtgcaacg
    10261 atacgcatat tcgctcctaa aattgcacct gtgactaata aagagttagc catattgttt
    10321 cgaccatctc cgcagtaagc aacagtgata ccttctactg ttccaaagtt ttcttggata
    10381 gttaaaaagt cagcgatcat ttgtgttgga tgccattcat cagtcaagcc attccaaact
    10441 ggaacaccag agaattctgc tagttcctct accatttttt gactgaatcc acggaattcg
    10501 attccgtcaa acatacgtcc tagtacttta gctgtatctt ctgtggattc ttttttacct
    10561 aattggatat catttgcacc tagatattca ggatgtgcac ctagatcgat cgctgcagta
    10621 gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat attttttcct
    10681 tctagatagt gatgtgggat accacgtttt ttcaagtctt tcaagtgttc tgaaaaatcg
    10741 atcaaatatt gtaattcttc ttttgtaaag tctttttctg ctaataagct tcttccttgg
    10801 aatactgatt ctttcatatt aaacattctc ctttagattg tttttattta agattaaaga
    10861 ggaaatcccg acggcagctg acagtaagac tgccgtcagg aaacaaatta cttcaagtct
    10921 tcacggacaa gtggttggct catgcagcgt ggaccaccac gacctcgtga caattcactt
    10981 gaattgattt ctaacacttt gatgccatag ctacgaagca attcgttcga tacataattt
    11041 cggttataag tgactacaac acctggagcg attgccaatg tgtttgatcc atcattccat
    11101 tgttctcgag gtgcaacgat ctcatctcca ttaccagttg ggatcagtac aagatcatct
    11161 aatccaagag cagctttcat cgtttcgtgt aagttatctg aatgagtaat cttgatatca
    11221 tcaccatctg gttcgattgt aaagacatcc atcttaccgt ctttactttg aattgctgga
    11281 tggatagtga atttatcgtg atctaccatt gtaaatacgg tatccaaatg catcattgca
    11341 cggttgttcg gaattttgat tgccaacact ttttcaaatc ctgaattttt tgcgaatagg
    11401 tggcgtgcta atttttctaa agctttcgca ttagttcgtt gagaaatccc aacagctact
    11461 actttatcgc ttaagatcaa ttcgtctccg ccttcgatat gatctggatt ttcacgatcc
    11521 aaccatactt cgattccttt atttgcaaat cttggatgat gtttcaaaat atattctgta
    11581 aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat cccatttccc
    11641 attgatgcgg aagggtcgcg tgtaaagtac aagtttggca ttgggtccat gtaaaatgga
    11701 tagtcatcgt ctgctgacag atcatataga ctgctagaac gtacttcgat atcttttgta
    11761 cgaaccccag ccatgatttt atctaccatt tcttgcgttt ccattgatag aaggaactca
    11821 tgaagaccct ctcttacagc attcgatgca atatgagatt catccaacat tttattcaag
    11881 aattgttctt tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc caagtaaaga
    11941 gtctcgacgc cttcattttg caatgtcttc gcaaagttgt catgttcttc ttgagcaatc
    12001 ggaagatatg gaatatcatc gaacaacaga cgatccataa tatctggtgt caaattttcc
    12061 acttcttgtc caggccgttt cagtaaaact gtctttaatt tccctatttc agagaaaacg
    12121 tgaataggtt tatccatgtt gtgttgcctc cttctttttg attacatgta taggatatcc
    12181 tccttttgta atcactttca taattttgtc tcattcaaaa tgagacattc ctcacattaa
    12241 tattcgataa ttaaataatt aaacgttttt ctatttttaa gcgtaattta ttcatttttt
    12301 attctgtata cactttcaaa aaaatattta ttcattgaaa aaattttttt gaataaatta
    12361 aaaaaataaa ttatattatc gttgttagcg tttactatat gtattattgt tgttttttat
    12421 acacaaatca ttcgcttcaa aacaaattat tcaataccat tgtatatttt ttacataata
    12481 acaaattata tttgtaaagt attaaaactt gtttctaatg ttggtatgtt aaaactttac
    12541 aaattcttta aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg
    12601 cgtgttaata aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat
    12661 ttctcggatg aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa
    12721 ggacagatcc ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga
    12781 ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat
    12841 acaaataacc tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct
    12901 caagcgatga ctgatattga aacattctac cttcctgttt cctactatga aaaattagca
    12961 cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata
    13021 catgaattgc gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat
    13081 ttattcattt tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct
    13141 tatccgatca ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga
    13201 caagtaatca aacaactacg tgattccggg ctgttagatt atgacaaaaa gcgttttact
    13261 ttctgtaaga cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg
    13321 accctgataa atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg
    13381 cagttttaga tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata
    13441 ttcactgttt gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga
    13501 tctagattat tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa
    13561 cggataatga taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt
    13621 aaaaaatagg gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc
    13681 tgtcaactct tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat
    13741 tgttccttgt gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca
    13801 aaagcttgca tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc
    13861 tgggctgttc ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa
    13921 accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca
    13981 tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact
    14041 agttctacct cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga
    14101 ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga
    14161 agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt
    14221 ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt
    14281 aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa
    14341 ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct
    14401 aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg
    14461 atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga
    14521 agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc
    14581 atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa
    14641 ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa
    14701 tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct
    14761 acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt
    14821 tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt
    14881 tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt
    14941 ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt
    15001 tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac
    15061 gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca
    15121 aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga
    15181 cttcatctat tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa
    15241 gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg
    15301 aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat
    15361 caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat
    15421 ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc
    15481 ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg
    15541 tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc
    15601 gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg
    15661 ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag
    15721 gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat
    15781 agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta
    15841 cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa
    15901 agcttctggc atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc
    15961 tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc
    16021 tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc
    16081 catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat
    16141 ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac
    16201 aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag
    16261 cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta
    16321 tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta
    16381 tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa
    16441 ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca
    16501 aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt
    16561 atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta
    16621 tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt
    16681 gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac
    16741 atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat
    16801 cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa taatcaactc
    16861 tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt
    16921 ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa gcgagggcgt
    16981 tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt ctggtaccac
    17041 taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa
    17101 aagcgcatca ggaacatgcg gattcgtttt taaatactgc ttcactgctt gatatcctga
    17161 ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt ctgccaaaac
    17221 ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct tcgtatattc
    17281 tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt caatgacttt
    17341 ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag tgccgtcaga
    17401 atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag cttcgtcgaa
    17461 ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc acgtttcttt
    17521 caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa tagataaata
    17581 ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt
    17641 agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg cttcaaaaat
    17701 tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa ctcttgaaac
    17761 agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca tttgtctctc
    17821 ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg tcctttttca
    17881 tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc tacagatttt
    17941 tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt taattgttct
    18001 gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa atgctctact
    18061 tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac ttcgatcaac
    18121 ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat cccatcagga
    18181 tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta attgtccctt
    18241 cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata
    18301 gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc tgcttcaata
    18361 agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt cacgactcct
    18421 gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc ttttgccata
    18481 ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc tccaacaatt
    18541 ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt
    18601 ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa aatgatcgaa
    18661 tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc gtctagattc
    18721 attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct atatccttcg
    18781 ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg ttctgctgcg
    18841 atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag ttcaccatac
    18901 tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat gtagtcattt
    18961 ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga atagtgctgt
    19021 gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca
    19081 aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc cttagcgaca
    19141 agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat gctttctaga
    19201 gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc catttccatc
    19261 catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact gattgacata
    19321 tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc catgctgttt
    19381 tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct ctcgcttttt
    19441 cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt tctgctggaa
    19501 tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag tatcctttgc
    19561 tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc aaagctaaaa
    19621 tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt ccatctggtg
    19681 tatcataatc gtctccataa acagccaagg cttttctttg accaagagct acttgtaaaa
    19741 tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca tctgcttttg
    19801 ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct tggtcacacc
    19861 acttcatcat tttttccatc atcaatttac tttcaccata cggattttta ggattagctg
    19921 gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca gtagaagaga
    19981 atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc ataccgtata
    20041 cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa gagcttgcag
    20101 caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga aattctttat
    20161 cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg tgacctgtga
    20221 gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct acagcgtgcg
    20281 aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact cctttgatta
    20341 gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt ttatggtgaa
    20401 aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa catttagtaa
    20461 acattttcaa ggattttttg caaaggttta caaacaaacg taaaactagc ctttttttct
    20521 cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg aaaaaatttg
    20581 tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt ttggttgctt
    20641 ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc gaagaaaacc
    20701 gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat acgcatgaag
    20761 actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca atataagttg
    20821 tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag ctactgctgc
    20881 agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag actggatttc
    20941 ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt ctgaatgata
    21001 acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct tcaaattttt
    21061 tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt caatcaaata
    21121 gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct tttgctgaat
    21181 atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg ctaattcttt
    21241 cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa accatcccca
    21301 tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct gccggattgg
    21361 atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc aattgccttt
    21421 ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa agggctcatt
    21481 ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga ttagtgctgg
    21541 atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa aatttttctt
    21601 tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact caacgtcttt
    21661 tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc attgctccag
    21721 gttttcattt gttagtaacc gtggccatgc cattgctccg ccttgctttt gatttcttcc
    21781 tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt ccttgcctga
    21841 tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg atactgtaaa
    21901 caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat tttctttagg
    21961 cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca gtaaaacatt
    22021 tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt cccagtgacc
    22081 gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta aaagataacc
    22141 atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag aagctggaaa
    22201 ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac atcgagtaga
    22261 ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat ttttggttac
    22321 cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt tgggtccctg
    22381 tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt gttgagaaac
    22441 agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc caggaccatt
    22501 gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga gaatcctttc
    22561 tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt gcatgcctcc
    22621 atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt tgcaccataa
    22681 acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat agcttgcttc
    22741 ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc aaatcgttct
    22801 ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt caaccacaga
    22861 tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac gagtattcta
    22921 tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat agttgtttcc
    22981 ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat aaaaaaaatg
    23041 aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta atgattgatt
    23101 cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt gatttttcca
    23161 gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc tttacgtaat
    23221 ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac aggctggtct
    23281 tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct tgcacctaaa
    23341 atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc ttttctcatt
    23401 ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat tactcgtaga
    23461 tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt aattacccat
    23521 aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag gcagcaccaa
    23581 tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct cttacttgcg
    23641 ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct cctgctgccg
    23701 atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag tttccgcaag
    23761 ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga tcgtcttctt
    23821 gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc attgctttca
    23881 gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc acacctgtag
    23941 cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac ccttcaggat
    24001 ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc aaatttccgc
    24061 cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg tctggatcgt
    24121 tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca ttatcgatag
    24181 cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact tctgtccagt
    24241 ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct ggggtaccca
    24301 tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga tgattgattg
    24361 attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta tcgatgctcc
    24421 atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt gttccaccta
    24481 aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc tttcttcaat
    24541 tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat caatcaaacg
    24601 gctatcatac agattacgca attcgatagt cattaattca atgtcataga tgcgtgcacc
    24661 cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata agctctccat
    24721 ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc cagtaaaata
    24781 catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg tatcgaatag
    24841 cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc aattagccca
    24901 ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa tgtcaacaca
    24961 ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt aaaatgaaac
    25021 cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc accagacaga
    25081 acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct gaacatataa
    25141 atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat aaaatacagt
    25201 atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg tgtcactaaa
    25261 cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata ataagggatc
    25321 tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc taaaaaagca
    25381 taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg ataattcatg
    25441 aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat caaagctctc
    25501 cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt ttttatgaaa
    25561 attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac cggctctatt
    25621 gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga gcttctctga
    25681 ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat ccatttcata
    25741 aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt gttctttttc
    25801 tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag tcgtccctat
    25861 cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc gctgttcttt
    25921 ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt tccggcgtaa
    25981 tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg gtataggaaa
    26041 aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa aagaagacct
    26101 agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg tttttgtctg
    26161 ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat tcctgtaggt
    26221 acttccccac ttagtagaat cactctagca accatatctg cactaagcag aaaaatggaa
    26281 ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac tagcttcgca
    26341 gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac actaccgctt
    26401 gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt aaggcctaat
    26461 ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata aagtcctcct
    26521 agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca gctcgcaccc
    26581 cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga taattggatc
    26641 gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga caataagatt
    26701 cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca gcaagcagct
    26761 aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata gtgcgaaaag
    26821 aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa tatactagaa
    26881 tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc aagacaagct
    26941 cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat cagcgccaaa
    27001 gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat aaaaggcgta
    27061 ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa atagaacgtc
    27121 caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac ggaacgccaa
    27181 tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc ctcgctgtaa
    27241 aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga acataacgat
    27301 agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata aatgaaacag
    27361 gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa acaatcaacc
    27421 gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa tgaatcaaag
    27481 atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa ggaaacaata
    27541 ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa gcaagatcct
    27601 gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa gaactaatag
    27661 ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac tgcaaaaaac
    27721 caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac acagaaaaat
    27781 cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca cccgcgttga
    27841 ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc aatgcaccgc
    27901 tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa cgaatatttc
    27961 gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc caaaaatctg
    28021 cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc aaaagaacaa
    28081 aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat cctcctgctc
    28141 ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa gcgtgacaat
    28201 cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt cttcaggtgt
    28261 accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa aacgtgaagc
    28321 atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat tgatttcagt
    28381 caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg gttcatccaa
    28441 gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct gggtttgtcc
    28501 accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag catccaacgc
    28561 ccactcgatt gcttcatgat cttgcgtgtc catgccactg aacagttttt gataagggta
    28621 tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac tagtttgtgc
    28681 taataatgca attttttttg caagttcttt tgaactgtat gtagagatat cagagttatt
    28741 gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca ataaagtaga
    28801 ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa tcgtaacaga
    28861 catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg ctcttaatct
    28921 cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg tttcaacatt
    28981 tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg ttcgaaatat
    29041 caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga aatgaaatta
    29101 aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact gagcgcttgc
    29161 tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc atccaatagt
    29221 actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc gcccaaacgg
    29281 atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc tatcgcccag
    29341 tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa ggatgtccca
    29401 acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga tttgctgttg
    29461 atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa aatcgcgcca
    29521 acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa agatgtcggc
    29581 aaagctttgg caaaagaaaa agaggcagaa aagatcattt ccgattatca aaaatccatc
    29641 aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc agtgttgtgg
    29701 gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag aatcgtatac
    29761 gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa agccacttct
    29821 gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta catcatcctt
    29881 gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa tcttaaagca
    29941 gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata taacggacct
    30001 attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt ttaactaaaa
    30061 aaagcatctt cacgattagt cgtgatatgc tttttttctt attaacaaac tagacgcata
    30121 gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt tgaaaacgat
    30181 gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg gcgaagtctg
    30241 aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc aatgttcgcc
    30301 agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc gaaacataga
    30361 tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc attacttccg
    30421 cattttcttt cgtgcgaaaa tttggctgcg ttgtcaacac gatgatatgg cgataaggaa
    30481 gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt cgatccattg
    30541 tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa ccatttttgc
    30601 cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc gctccttatg
    30661 attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg aattgagaac
    30721 tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt gggcaatatt
    30781 tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga tagttacgtt
    30841 ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct cccattttta
    30901 caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt ttctagattg
    30961 ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca acggttttac
    31021 ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt atatagatta
    31081 tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt tcctgcatca
    31141 atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa tgcattatta
    31201 gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc aaaaaaatgg
    31261 tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa tccaagcaag
    31321 acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga tgtgatcgta
    31381 gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc aagcaaccat
    31441 tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa caaagaatca
    31501 actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta ttgaacatat
    31561 gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac aatggtgttc
    31621 ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc tgttttttga
    31681 aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt attgatattt
    31741 gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt ttcatctaca
    31801 aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct ttgctcttct
    31861 ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc aaaaaattct
    31921 tttgcaaaat ctgcttcgaa caatgctttg acttgttcgt agccgtgttc ttctttcaac
    31981 caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattt ttccggaaga
    32041 tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa taaaaactta
    32101 cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac tcggacttcc
    32161 aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg catccattga
    32221 tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt tttctcgtaa
    32281 tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt taacacttct
    32341 gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt tgcaggagca
    32401 agtgattcat ataaatggat ccgtccatgg aaatttttca tttttccgat tttacgtcct
    32461 aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac acggatgaat
    32521 tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc cgccccttct
    32581 ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta caaaggtacg
    32641 caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa tgctttgaac
    32701 gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc ggttagtttt
    32761 ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa agaatgatct
    32821 ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa agaaccaact
    32881 tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc gattttttcg
    32941 atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca atattgctga
    33001 atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg atagacatct
    33061 ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg tttcaacgct
    33121 tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat ttctgaacca
    33181 aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa gtggtctctt
    33241 gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat tccgcggatc
    33301 ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc tgtatctgca
    33361 tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg gtaattatag
    33421 atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg ctgagtaccg
    33481 ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt atcgatatat
    33541 tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt aataattgtt
    33601 tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact ttcacatcct
    33661 aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa atagcggacg
    33721 aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt tctgttttta
    33781 cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc taccttattc
    33841 tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca aaaaaagaca
    33901 cgacctaaag atcgtgtcta attatgactg tatcagaaaa tatgatacca aacggatcta
    33961 ttcattaatt aattgacata gtttatctgt tgctctttta tgactggata actgaccaat
    34021 tcatgacgat ccaagtcttc tttattcata tttacagcag taatttgact gtcttcatac
    34081 gtacgataat aataaatccc tttatcaacg ttacagcaag aagaataaat cgtatattcg
    34141 tattttcttt ctccagtatc gcatactcct ttttgttgct cgactgagcc tagtatatga
    34201 aaaaattgac tgatactttc agattctgaa tcacctgatg cagcattcat tttagtaaat
    34261 gtggctttta caaatcgaga aacagaagag agatctccag gtaagcccaa tcctcccatc
    34321 ccacgactat aaacattcaa gctgatctgg tttgaaaaat gattttctgg tgttccattt
    34381 gatagcgagc gataattgtt cagattaaat aattgataat caaatggcgg attatttgtc
    34441 aacacaccga caggattatc atataaatga agtccatctt tcatactttc aattactatc
    34501 gatttttctt tatcagctaa taaccagtgt aaaggtgaca agggtagttt ttcactaaaa
    34561 tcgatattga ctagattgat agtagccaat aattctttag cttcgtctat ggttgagcat
    34621 tgccctagta tccatggaat aaactcaaat ggtgcaacgt tatcttttcc ttcttgtaat
    34681 tctttataat cagcattgcc aggaaaattc agtcctgcca tactcaaccc tttttcattt
    34741 gtagcctcat aatagagtgg atagttggat accccagctg ctattcctat cattgcataa
    34801 tgtttatcta aattttttac ctttctaaaa tcgaaacgat aatttcttgg agtaatagtg
    34861 accacttcat tataagacat ctcataatca aaattccttc caaaataatg gtctttcgtt
    34921 gcataagtga ttgcggtaca catcgtttcc cctcctaaaa caaatcgttt ttacactttt
    34981 atcttacatc tagtattttt tttatcaaaa aaaggccaaa gaagattgat ttactagcat
    35041 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    35101 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    35161 ttgcatctgc ggtaataagc ccaatcaaac gaaaaaatag gaaaagctat ttttgtttgg
    35221 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    35281 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
    35341 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
    35401 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
    35461 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
    35521 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
    35581 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
    35641 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
    35701 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
    35761 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
    35821 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
    35881 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
    35941 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
    36001 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
    36061 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
    36121 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
    36181 ttcagtcttc tttaactttt ttttgcaaaa ttaaaaaaat ttggttaaat tcaatgggtt
    36241 cccttacatt tttagttttg acctaaatga tagttgtcca aatctttcat ttcaagttat
    36301 agttaaagaa aatgaaaaag aaggtgtgta atggaaaacg aaactgacgt tatttatatt
    36361 catcctcaaa aacgaatcgt ttcacagaaa agaaagtatt tttatcttgg ttttacaggt
    36421 gttttttttc tttttattgg cttgctttct aatactccta ctgataattg gagtggattg
    36481 ctaactattc ttacctcgcc aagcaatctt ttgacagatt attttgcttt aggcggtttc
    36541 ggaagcgcat tcataaatgt agggattttg accttacttt ctgtgctttt agcttatcgg
    36601 cacaaggtga ttttaaacgg tcccttattt gcttcgattc taacagttac cggtttctct
    36661 ttttttggca aaaaccttta taattctatc tcaatcattt taggtgtgta tctatacgct
    36721 gtatttgtca ataaaccttt ttctcagtac atcatgatcg gactgtttgg tagcgcatta
    36781 tcaccggtcg ttagttatat cacttttggt atgcgttttc ctttactgat aggtatttta
    36841 ttggggaatt tagctggcat agcaattggt ctacttcttc cgcccttagc tgctcaaact
    36901 ttagttttcc atcgtggatt cacactttat aatattggat tcacctctgg gttgattgcg
    36961 atgactttta ctgctgtttt gaggttattc agctattcaa tcgtcgaaaa tacattggtt
    37021 ttcaatgaat atcactttcc tctcatttgg attatttttg gctttttttc gctgacagtc
    37081 ggaattggtt tctattataa ttcctttcgg cttttgggga ttcgagaaat tttcgattca
    37141 tctggaaaat tgacaactga tttcattgct aattctggta ttggtgctac tttgatcaac
    37201 ataggcttag ttggtctgat gctgtcttct tatgttttgc tggttggcgg tcagttaaat
    37261 ggacctgtca tcggtgctat tttatctgcg gtgggtttca gtgcttttgg ttgtcattta
    37321 aaaaacagtt ttccgatact agtcggaata tttattgctt ctttgtttgg aacttttcat
    37381 gagatcacca gtactggcat gcttgttgca gctgtgtttg ggacaggtct cgcgcccata
    37441 agcggttttt acggcagttt ttacggtgta atagctggtg tactacatat tgctcttgtc
    37501 cataacgtca gcactttgca cggaggacta aatctatata acagtggttt ttcaactggt
    37561 ttcgttgcag gtatccttgt accgatacta gataacttta ccgctgtcag aaaggaaaaa
    37621 aagacacttg gaaaaagaat tatcaaaaag aatcatcgat gagcttctta cttatttata
    37681 ttcccatgaa ataactgaaa tacgtattgg agtagaattt gctgaggaag ggcttttcat
    37741 agagatacag ggtacggcag aaactcagcc agaagattta gatgtacttg ctgatttgct
    37801 gaacaatcct cgggacccta gtatggaaat ctattatcaa gaactattag gtaatccccg
    37861 acatgatcgg gaagattatc atctgttagg tagtttgatt gatgacgtgg aaatcatgta
    37921 tgcccagccg attatcaata ttaagatctt tcgacgtgct tctcattcct attattaacc
    37981 ctattttcta tttataaaag actgaatcaa aaaagcagct cctgaaagtt cgattaaaat
    38041 ctactttcag gagctgttca agcttgacat tattgatttt ccaatccttc atacacttca
    38101 tctgttacag gttctaaaaa ctctgaatta ccagcagtga tcgccaaatg ttcaaaccaa
    38161 ctgtcttttg tcgcaccatg ccaatgttta atccctttgt gcgttacgac gacatctcca
    38221 gccattaatc gtcttgctgg ctttccttct tcctgatacc agccttctcc tccggtcaca
    38281 agtaagattt gatagccgtc tttatggaca tgccagtgat tgcgacaacc tggttcaaaa
    38341 gttacattac ctacaccaac agaaacttcg ggatcattca ctaacatatt cagatagctt
    38401 tacccggtaa atacttgagc gtaagcttcg tttttttcac ctgctggaaa aatcacgcca
    38461 ttgtgtattt cttcgtgttt tgtcgtcatt tacgcctctt tcttatcttc gctattttct
    38521 tctaaataga tttcttttgc taaactaaaa gctgaccaag cttttggcca tcctacataa
    38581 aaagctaaat gggtaatgat ttctgctatt tcttcactag taatgccatt ttgcttgcca
    38641 atcttcagat gagctggcaa ttgttcaaaa tttcctcctg aaattaatgc agatatggtg
    38701 atgatactac gctgatgagc agataattct tctctggacc atacttgtcc aaataaaaca
    38761 tcatcattca attctgcaaa ttttggtgca aaatctccta attgatctcg tcctgcggtt
    38821 tgtttttctg ccatttctct tattctccct tcacgactgt taggtgattc aactatggtt
    38881 ctaaggcaac tttattttct attgtttctt cgcatttcat tttaaatgta gtctttcagc
    38941 ctgtccaatg cttagtttat tcagtcgata tgctttttaa gcatagttgc taacgctttt
    39001 tttat
//
LOCUS       DACERL010000022        35882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.023, whole genome shotgun sequence.
ACCESSION   DACERL010000022 DACERL010000000
VERSION     DACERL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.023"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..1152
                     /locus_tag="IXF54_001122"
     CDS             <1..1152
                     /locus_tag="IXF54_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404397.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXF54_001123"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXF54_001123"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1404398.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXF54_001124"
     CDS             2728..3003
                     /locus_tag="IXF54_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1404399.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXF54_001125"
     CDS             3009..3776
                     /locus_tag="IXF54_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1404400.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXF54_001126"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXF54_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1404401.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXF54_001127"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXF54_001127"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1404402.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXF54_001128"
     CDS             7920..8222
                     /locus_tag="IXF54_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1404403.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXF54_001129"
     CDS             8225..9388
                     /locus_tag="IXF54_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1404404.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXF54_001130"
     CDS             9414..12842
                     /locus_tag="IXF54_001130"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1404405.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXF54_001131"
     CDS             12863..13987
                     /locus_tag="IXF54_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404406.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXF54_001132"
     CDS             13977..14414
                     /locus_tag="IXF54_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1404407.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXF54_001133"
     CDS             14398..14736
                     /locus_tag="IXF54_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1404408.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXF54_001134"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXF54_001134"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1404409.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXF54_001135"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXF54_001135"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1404410.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXF54_001136"
     CDS             15893..16936
                     /locus_tag="IXF54_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1404411.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IXF54_001137"
     CDS             17095..17823
                     /locus_tag="IXF54_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1404412.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IXF54_001138"
     CDS             17939..18184
                     /locus_tag="IXF54_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1404413.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IXF54_001139"
     CDS             18181..18399
                     /locus_tag="IXF54_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1404414.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IXF54_001140"
     CDS             18535..19074
                     /locus_tag="IXF54_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1404415.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IXF54_001141"
     CDS             19067..21340
                     /locus_tag="IXF54_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1404416.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IXF54_001142"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IXF54_001142"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1404417.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IXF54_001143"
     CDS             complement(22513..22767)
                     /locus_tag="IXF54_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1404418.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IXF54_001144"
     CDS             complement(23167..23436)
                     /locus_tag="IXF54_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404419.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IXF54_001145"
     CDS             complement(23906..24361)
                     /locus_tag="IXF54_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1404420.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IXF54_001146"
     CDS             complement(24499..26427)
                     /locus_tag="IXF54_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1404421.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IXF54_001147"
     CDS             complement(26624..27457)
                     /locus_tag="IXF54_001147"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1404422.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IXF54_001148"
     CDS             complement(27649..28353)
                     /locus_tag="IXF54_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1404423.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IXF54_001149"
     CDS             complement(28376..29002)
                     /locus_tag="IXF54_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1404424.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IXF54_001150"
     CDS             29328..31703
                     /locus_tag="IXF54_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1404425.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IXF54_001151"
     CDS             complement(31727..32218)
                     /locus_tag="IXF54_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1404426.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IXF54_001152"
     CDS             32405..33325
                     /locus_tag="IXF54_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1404427.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IXF54_001153"
     CDS             33479..33823
                     /locus_tag="IXF54_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1404428.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IXF54_001154"
     CDS             complement(34013..34513)
                     /locus_tag="IXF54_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1404429.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IXF54_001155"
     tRNA            35041..35124
                     /locus_tag="IXF54_001155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IXF54_001156"
     CDS             complement(35448..>35882)
                     /locus_tag="IXF54_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1404430.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11869 a         6261 c         7404 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACERL010000023        35376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.024, whole genome shotgun sequence.
ACCESSION   DACERL010000023 DACERL010000000
VERSION     DACERL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35376
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.024"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(229..2121)
                     /locus_tag="IXF54_001157"
     CDS             complement(229..2121)
                     /locus_tag="IXF54_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404431.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="IXF54_001158"
     CDS             complement(2118..3845)
                     /locus_tag="IXF54_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404432.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="IXF54_001159"
     CDS             complement(3861..4511)
                     /locus_tag="IXF54_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1404433.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="IXF54_001160"
     CDS             4715..5011
                     /locus_tag="IXF54_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1404434.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="IXF54_001161"
     CDS             5183..5803
                     /locus_tag="IXF54_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1404435.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="IXF54_001162"
     CDS             complement(5895..7061)
                     /locus_tag="IXF54_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1404436.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="IXF54_001163"
     CDS             complement(7073..7426)
                     /locus_tag="IXF54_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1404437.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="IXF54_001164"
     CDS             7554..8576
                     /locus_tag="IXF54_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1404438.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9015..9449)
                     /locus_tag="IXF54_001165"
     CDS             complement(9015..9449)
                     /locus_tag="IXF54_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404439.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="IXF54_001166"
     CDS             complement(9559..11418)
                     /locus_tag="IXF54_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1404440.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="IXF54_001167"
     CDS             complement(11536..12696)
                     /locus_tag="IXF54_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1404441.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IXF54_001168"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IXF54_001168"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1404442.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="IXF54_001169"
     CDS             complement(14443..15141)
                     /locus_tag="IXF54_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1404443.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="IXF54_001170"
     CDS             complement(15098..16759)
                     /locus_tag="IXF54_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1404444.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="IXF54_001171"
     CDS             16977..18377
                     /locus_tag="IXF54_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1404445.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="IXF54_001172"
     CDS             complement(18629..19708)
                     /locus_tag="IXF54_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1404446.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="IXF54_001173"
     CDS             complement(19794..20753)
                     /locus_tag="IXF54_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1404447.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22288)
                     /locus_tag="IXF54_001174"
     CDS             complement(20765..22288)
                     /locus_tag="IXF54_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1404448.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="IXF54_001175"
     CDS             complement(22298..22861)
                     /locus_tag="IXF54_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1404449.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="IXF54_001176"
     CDS             complement(22867..23730)
                     /locus_tag="IXF54_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404450.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="IXF54_001177"
     CDS             complement(23744..24640)
                     /locus_tag="IXF54_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404451.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26086)
                     /locus_tag="IXF54_001178"
     CDS             complement(24815..26086)
                     /locus_tag="IXF54_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404452.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="IXF54_001179"
     CDS             complement(26076..27593)
                     /locus_tag="IXF54_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1404453.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27791..28681
                     /locus_tag="IXF54_001180"
     CDS             27791..28681
                     /locus_tag="IXF54_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1404454.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IXF54_001181"
     CDS             complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IXF54_001181"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1404455.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29590..31062)
                     /locus_tag="IXF54_001182"
     CDS             complement(29590..31062)
                     /locus_tag="IXF54_001182"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1404456.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31699..32061)
                     /locus_tag="IXF54_001183"
     CDS             complement(31699..32061)
                     /locus_tag="IXF54_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1404457.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32284..33036
                     /locus_tag="IXF54_001184"
     CDS             32284..33036
                     /locus_tag="IXF54_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1404458.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33176..34099)
                     /locus_tag="IXF54_001185"
     CDS             complement(33176..34099)
                     /locus_tag="IXF54_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1404459.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34114..34386)
                     /locus_tag="IXF54_001186"
     CDS             complement(34114..34386)
                     /locus_tag="IXF54_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404460.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            35063..35284
                     /locus_tag="IXF54_001187"
     CDS             35063..35284
                     /locus_tag="IXF54_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404461.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PITAKKETVFSEGKIKTRYILEVETKFKN"
BASE COUNT        10502 a         7325 c         6074 g        11475 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    27721 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
    27781 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
    27841 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
    27901 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
    27961 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
    28021 cgataaaatc gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
    28081 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
    28141 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
    28201 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
    28261 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
    28321 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
    28381 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
    28441 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
    28501 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
    28561 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
    28621 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
    28681 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
    28741 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
    28801 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
    28861 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
    28921 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
    28981 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
    29041 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
    29101 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
    29161 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
    29221 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
    29281 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
    29341 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
    29401 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
    29461 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
    29521 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
    29581 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
    29641 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
    29701 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
    29761 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
    29821 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
    29881 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
    29941 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
    30001 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
    30061 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
    30121 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
    30181 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
    30241 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
    30301 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
    30361 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
    30421 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
    30481 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
    30541 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
    30601 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
    30661 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
    30721 caatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
    30781 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
    30841 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
    30901 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
    30961 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
    31021 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
    31081 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
    31141 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
    31201 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
    31261 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
    31321 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
    31381 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
    31441 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
    31501 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
    31561 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
    31621 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
    31681 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
    31741 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
    31801 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
    31861 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
    31921 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
    31981 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
    32041 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
    32101 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
    32161 acaaaaggat ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac
    32221 aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
    32281 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
    32341 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
    32401 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
    32461 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
    32521 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
    32581 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
    32641 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
    32701 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
    32761 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
    32821 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
    32881 tggttatctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
    32941 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
    33001 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
    33061 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
    33121 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
    33181 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
    33241 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
    33301 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
    33361 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
    33421 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
    33481 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
    33541 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
    33601 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
    33661 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
    33721 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
    33781 accttcgctc tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat
    33841 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
    33901 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
    33961 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
    34021 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
    34081 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
    34141 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
    34201 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
    34261 ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt
    34321 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
    34381 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
    34441 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
    34501 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga
    34561 tatttacttt taatagataa aatattataa ctattaaaaa taactatttt cattataatt
    34621 acacacaaaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    34681 gtataaacag cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    34741 gaaaagcaat ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc
    34801 tctttttatc tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca
    34861 tcataccgcc catcaagaca aacgttttta gcttgtacgt actggacaaa aatcccatta
    34921 tatcaagaaa aaacaacgtt gataatactt aaaattatta ggctagaacg gactagtaag
    34981 agttttttgc cccttttttg cccctcaaat atcgctttac agtcagaaca taagttcgta
    35041 taatcgtttt gaggtgattt ttatgatgga agactttatt agaaaaaata tcagtgatga
    35101 atatgcagac ttttatgagc aaagcagcaa aaaagataaa ttccagatgg atgtttcaat
    35161 tttagctata ttagcttttt ccgaaaataa acaacctata actgccaaaa aagaaacggt
    35221 attctctgaa ggtaaaataa aaactcgata tatattagag gtagaaacta agtttaaaaa
    35281 ctgattggag taatggttat gctttttaat gagacacaat tatggtttaa atttgaccct
    35341 tcgaatagat ttatcaaaga tttttataag gtgtgg
//
LOCUS       DACERL010000024        34789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.025, whole genome shotgun sequence.
ACCESSION   DACERL010000024 DACERL010000000
VERSION     DACERL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.025"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(233..1081)
                     /locus_tag="IXF54_001188"
     CDS             complement(233..1081)
                     /locus_tag="IXF54_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1404462.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IXF54_001189"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IXF54_001189"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1404463.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IXF54_001190"
     CDS             complement(3582..3761)
                     /locus_tag="IXF54_001190"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1404464.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IXF54_001191"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IXF54_001191"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1404465.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IXF54_001192"
     CDS             5909..8188
                     /locus_tag="IXF54_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1404466.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IXF54_001193"
     CDS             complement(8281..8928)
                     /locus_tag="IXF54_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404467.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IXF54_001194"
     CDS             complement(9107..9826)
                     /locus_tag="IXF54_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1404468.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IXF54_001195"
     CDS             complement(9957..11108)
                     /locus_tag="IXF54_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1404469.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IXF54_001196"
     CDS             complement(11325..12065)
                     /locus_tag="IXF54_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1404470.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IXF54_001197"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IXF54_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1404471.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IXF54_001198"
     CDS             complement(12449..13042)
                     /locus_tag="IXF54_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1404472.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IXF54_001199"
     CDS             complement(13026..13673)
                     /locus_tag="IXF54_001199"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1404473.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IXF54_001200"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IXF54_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1404474.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IXF54_001201"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IXF54_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1404475.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IXF54_001202"
     CDS             complement(15119..15646)
                     /locus_tag="IXF54_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1404476.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IXF54_001203"
     CDS             complement(15826..16611)
                     /locus_tag="IXF54_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1404477.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IXF54_001204"
     CDS             complement(16604..17473)
                     /locus_tag="IXF54_001204"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1404478.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IXF54_001205"
     CDS             complement(17474..18853)
                     /locus_tag="IXF54_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1404479.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IXF54_001206"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IXF54_001206"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1404480.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IXF54_001207"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IXF54_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1404481.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IXF54_001208"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IXF54_001208"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1404482.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IXF54_001209"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IXF54_001209"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1404483.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IXF54_001210"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IXF54_001210"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1404484.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IXF54_001211"
     CDS             complement(22811..23035)
                     /locus_tag="IXF54_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1404485.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IXF54_001212"
     CDS             complement(23088..24050)
                     /locus_tag="IXF54_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1404486.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IXF54_001213"
     CDS             complement(24060..24500)
                     /locus_tag="IXF54_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1404487.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IXF54_001214"
     CDS             24806..25771
                     /locus_tag="IXF54_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1404488.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IXF54_001215"
     CDS             complement(25828..26058)
                     /locus_tag="IXF54_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1404489.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IXF54_001216"
     CDS             complement(26144..27031)
                     /locus_tag="IXF54_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1404490.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IXF54_001217"
     CDS             complement(27120..28343)
                     /locus_tag="IXF54_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1404491.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IXF54_001218"
     CDS             complement(28360..29439)
                     /locus_tag="IXF54_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1404492.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IXF54_001219"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IXF54_001219"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1404493.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IXF54_001220"
     CDS             complement(31297..31563)
                     /locus_tag="IXF54_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1404494.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IXF54_001221"
     CDS             32042..34276
                     /locus_tag="IXF54_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1404495.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IXF54_001222"
     CDS             34424..34744
                     /locus_tag="IXF54_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1404496.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9843 a         7509 c         6064 g        11373 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DACERL010000025        33676 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.026, whole genome shotgun sequence.
ACCESSION   DACERL010000025 DACERL010000000
VERSION     DACERL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33676
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.026"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            493..1200
                     /locus_tag="IXF54_001223"
     CDS             493..1200
                     /locus_tag="IXF54_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1404497.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IXF54_001224"
     CDS             complement(1259..1903)
                     /locus_tag="IXF54_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1404498.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IXF54_001225"
     CDS             2030..2317
                     /locus_tag="IXF54_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404499.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IXF54_001226"
     CDS             2425..2745
                     /locus_tag="IXF54_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1404500.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IXF54_001227"
     CDS             2826..4139
                     /locus_tag="IXF54_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1404501.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IXF54_001228"
     CDS             4136..4855
                     /locus_tag="IXF54_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1404502.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IXF54_001229"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IXF54_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1404503.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IXF54_001230"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IXF54_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1404504.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IXF54_001231"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IXF54_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1404505.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IXF54_001232"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IXF54_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1404506.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IXF54_001233"
     CDS             10292..11185
                     /locus_tag="IXF54_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404507.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IXF54_001234"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IXF54_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1404508.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IXF54_001235"
     CDS             13122..13979
                     /locus_tag="IXF54_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1404509.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IXF54_001236"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IXF54_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1404510.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IXF54_001237"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IXF54_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1404511.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IXF54_001238"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IXF54_001238"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404512.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IXF54_001239"
     CDS             16735..17493
                     /locus_tag="IXF54_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404513.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IXF54_001240"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IXF54_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1404514.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IXF54_001241"
     CDS             18406..19437
                     /locus_tag="IXF54_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1404515.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IXF54_001242"
     CDS             19434..20201
                     /locus_tag="IXF54_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1404516.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IXF54_001243"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IXF54_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1404517.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22169..22486
                     /locus_tag="IXF54_001244"
     CDS             22169..22486
                     /locus_tag="IXF54_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1404518.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22486..22836
                     /locus_tag="IXF54_001245"
     CDS             22486..22836
                     /locus_tag="IXF54_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1404519.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23003..23941
                     /locus_tag="IXF54_001246"
     CDS             23003..23941
                     /locus_tag="IXF54_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1404520.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23956..24786
                     /locus_tag="IXF54_001247"
     CDS             23956..24786
                     /locus_tag="IXF54_001247"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1404521.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24830..25855
                     /locus_tag="IXF54_001248"
     CDS             24830..25855
                     /locus_tag="IXF54_001248"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1404522.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25870..26808
                     /gene="galU"
                     /locus_tag="IXF54_001249"
     CDS             25870..26808
                     /gene="galU"
                     /locus_tag="IXF54_001249"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1404523.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26933..27373
                     /locus_tag="IXF54_001250"
     CDS             26933..27373
                     /locus_tag="IXF54_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1404524.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27376..27978
                     /locus_tag="IXF54_001251"
     CDS             27376..27978
                     /locus_tag="IXF54_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1404525.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28041..29144)
                     /locus_tag="IXF54_001252"
     CDS             complement(28041..29144)
                     /locus_tag="IXF54_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1404526.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29354..30355
                     /gene="ccpA"
                     /locus_tag="IXF54_001253"
     CDS             29354..30355
                     /gene="ccpA"
                     /locus_tag="IXF54_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1404527.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30710..31105
                     /locus_tag="IXF54_001254"
     CDS             30710..31105
                     /locus_tag="IXF54_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404528.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31105..31356
                     /locus_tag="IXF54_001255"
     CDS             31105..31356
                     /locus_tag="IXF54_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404529.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31399..31794
                     /locus_tag="IXF54_001256"
     CDS             31399..31794
                     /locus_tag="IXF54_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404530.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31791..33047
                     /locus_tag="IXF54_001257"
     CDS             31791..33047
                     /locus_tag="IXF54_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1404531.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33059..33382
                     /locus_tag="IXF54_001258"
     CDS             33059..33382
                     /locus_tag="IXF54_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404532.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33375..33641
                     /locus_tag="IXF54_001259"
     CDS             33375..33641
                     /locus_tag="IXF54_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404533.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11283 a         5791 c         7246 g         9356 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga
    22021 gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac
    22081 cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt
    22141 taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ctaataaaaa
    22201 tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt
    22261 tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa ttattttata
    22321 cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa
    22381 caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg
    22441 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa
    22501 cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc
    22561 catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg
    22621 tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg
    22681 tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa
    22741 cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc
    22801 aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg
    22861 cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa
    22921 acgttttttt taattggtag tacttttggc tagacaatga taaaatgata gagaacactc
    22981 taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa
    23041 attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg
    23101 ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg
    23161 tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg
    23221 tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg
    23281 attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg
    23341 ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct
    23401 tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat
    23461 tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca
    23521 tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg
    23581 agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt
    23641 aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta
    23701 tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt
    23761 tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt
    23821 tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc
    23881 aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta
    23941 gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt
    24001 cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc
    24061 agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg
    24121 ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag
    24181 tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc
    24241 ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg
    24301 tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg
    24361 ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt
    24421 ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca
    24481 acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga
    24541 aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt
    24601 ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc
    24661 cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg
    24721 cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt
    24781 atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag
    24841 agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa
    24901 cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca
    24961 acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac
    25021 caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc
    25081 aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca
    25141 tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga
    25201 aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga
    25261 agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa
    25321 atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat
    25381 cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca
    25441 cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat
    25501 cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat
    25561 aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt
    25621 gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg
    25681 tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat
    25741 cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga
    25801 aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag
    25861 cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt
    25921 ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc
    25981 cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga
    26041 aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta
    26101 agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg
    26161 catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc
    26221 tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta
    26281 ccattgacac agcaattgat cgatgactat gagcgtacac acgcttctac gattgccgtt
    26341 atgcaagttc ctcacgatga aacttcaaaa tacggaatca tcaacccagg ggaagtactg
    26401 gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca
    26461 agtgatttag ctattattgg gcgttactta ttgactccag aaatctttga tgtattatca
    26521 gaacaacaac caggtgctgg aaatgagatc caactaactg acgcaatcga tacgttgaat
    26581 aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttatgacgt tggtgacaaa
    26641 tttggtttta tgaaaacaag tattgaatat ggtctgacac atccagaagt aggggcacct
    26701 ttgcgtgaat acatcatgtc tttaggtaaa acattagaag cagaaggaag caagatcaat
    26761 cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataatc tttttttcta
    26821 acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa
    26881 aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg
    26941 aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg ttttcatcgt
    27001 tcgcgtattg ttgcaagttt ccaaaacttt agaaaaagta gaccagactg tcgcagaagc
    27061 aaatacgaca attgagatcg tcacacaaga cgtagatctt ttatctagac aagtagaagg
    27121 cttgctagtc aaaagtaatg agctgctctt ggatatcaat ggaaaagtcg caacgatcga
    27181 tccattgttc acagcagttg ctgatttaag tgaaagtgta tcggaactta atcattctag
    27241 taaaaacatc gtgacgaaag tcggcggaat cggaaaaacg acagctaaag cgactgttgc
    27301 cggaaaagtc ggtggaacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac
    27361 aggaggaaaa taagaatggc taaaaaaggt ggatttttat taggtgcagt agttggaggt
    27421 acagccgcag caatcgccgc attattgtta gcacccaaat ctggtaaaga acttcgggat
    27481 gaattggctg ctcaagcaga tgatttgatg gacatggctt cagattacac agatatggca
    27541 aaagaaaaat catctgagtt aacaggtatt gcaaaagaaa aagcgcaagg tctttctcaa
    27601 caagcagggg atctagcagg caatgttaag cagaaggtaa gtgaatcagc agaggaagca
    27661 ggaagtgaat cagatgatat cttgttaaac ctcaaagaac aatcagctga tttatctaat
    27721 cgtttcaaaa aaacagcaga agatctgaaa aagccagcag acgaattagg tagtattgct
    27781 aaagatactt cagaagatat catgatcgat gtaaaagatg ctgcagataa agcaggagag
    27841 acggtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgttg
    27901 aaaatggcaa aagaagatgc aaaagaagct aaaaaagaaa tgaaagatac aatgacagat
    27961 tcgactacca atcactaaga ttactcattt ctagaataaa aaaggtgaga gcggaccagc
    28021 tctcaccttt tttattttca ttactctaaa acaagtaatt cttttggtgt agtcgtgaat
    28081 ggcacacaac catcacttgt cacatacaca caatcttcga tacgtacccc tactttttca
    28141 ggaatataga ttcctggttc aagagagaag cacatccctt cttcaatcac taagtcgttt
    28201 ccttctacaa gagaagggaa ttcgtggacg gttgttccga ttccgtgacc taaacggtga
    28261 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    28321 cctgctgtca cacctgggcg aactgcctct tgtgcggcaa gctgtgcttc aagagtgatg
    28381 ttatagattt tttcttggaa ctcagttggt ttttgataag caactgtacg agtagcatca
    28441 ctgcaatagc cattccaaat aacgccaaga tcgaataaca ctagttcatt tggtgaaacc
    28501 ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    28561 tcgaaagaca tttgtgaaac accttgtttt tttaattggt attcgatttc tgcgataatt
    28621 gcttgttcag ttacgccttc ttttactgtt ttgaagccaa tttcaaaggc aacatcggcc
    28681 catttaccag cttcgatcag tttgtctttt tcttctaccg ttttgatcag ttgcagacgc
    28741 tggataatag aagttagatc ggtgtaagaa tctgtacctg tcaagatatc agagatagca
    28801 tcgaagcgat cgacagtcag agcattcttt tcaataccta cattaccagg ttgcccatag
    28861 cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt cgctgtctaa gtagccacgt
    28921 acatcgtacg cccagctgct gttttgagca tcttcaactt ccagagcagg agtgaaaagg
    28981 aaaggatctt gttccagagg gataaaaaga gctagtacac gctcgtgagg atcactttca
    29041 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc tacttggtgt
    29101 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    29161 taaattaatt ttgtattcaa taattgtacc actgattgat taaaagacca acgttcttaa
    29221 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttgttgagat
    29281 tatttgatat cctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    29341 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    29401 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    29461 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaatg cagtcgcacg
    29521 tggcttggca agtaagaaaa caacaacagt tggggtaatc attcctgatg tcagcaatat
    29581 gttctttgcc tctttagcac gtggtatcga cgatgtggca acaatgtaca aatacaatat
    29641 catcttagca aactcagatg gcaacgatca aaaagaagtc aatgtattga ataatttatt
    29701 ggctaagcaa gtggacggcg tgatctttat gggtcaccat atcacagacg aaatccgtgg
    29761 agaattttca cgttcaaaaa cgcctgttgt actagctggt tccattgatc cagatgaaca
    29821 agtaggtagc gtaaacattg attatgtgtc agcaacaaaa gatgcagtaa ataaattagc
    29881 aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg ctgattgatc ctatcaatgg
    29941 tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa aacaatctac catatagcga
    30001 aggattgatt tttgaggcac aatacaactt taaagatggt ctgtctctag ttgaccggat
    30061 ccataatagc ggtgcaacag ctgtctatgt ttcagacgat gaattagcca ttggtatctt
    30121 ggatggttta ttagaccgcg gcgtaaaagt accagaagaa ttcgaaatca tcacaagcaa
    30181 caattcttta ctgacagaag tagctcgtcc tcgtttgaca agtatcacac aaccattata
    30241 cgatttagga gcggtatcca tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    30301 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatcaacta aataataaaa
    30361 gatcaatcat ttaaaggttg ttccaaaagt ggtcaacttc tggaacaacg tttatacgag
    30421 aaagggggtt atgacaaaat tttgtcataa cccctttttt tttgtgactt taaaaaaaga
    30481 gaaaatggcg gaaacaaacc ttacgtccat cgtatacgac acaatctgtg cgtagatgct
    30541 acattttgtt cactactatc aaaattactt taaaatgaaa acgtgataaa aaagaaagta
    30601 gaaaagtaga taagtaactc taaaaaatcc aaaaaaatcc aggctcgatt gcgtacttat
    30661 cttaaaggag aaacaatcca tcatatataa cccgaaagga gacttctatt tgcctaagaa
    30721 aagacgaaag accatagtca tcattttgac cattcttact ataggaatcg gagggaaatt
    30781 ttacatggac aaaagagaaa tgcaaagaca gaaagattta ctcgcagtag aaaaacagtc
    30841 cgtcaaagtt ctgaaaaata cctttgcgga tatcaaggaa gtgaagattg aaaaatctgc
    30901 tcgaaatgaa atgacgggtt cataccggat agttattcta atgactaata aacaagatca
    30961 atcgatttat ttttcttata gtttttggaa agaaagaaat gagataggaa gttacggaat
    31021 agttgatgaa aaaaagcaaa aagaaggaaa tacgctcaat aaagttaaag taacttactc
    31081 taatggaaat gaggaaagta tttaatgatt actgacaaac aatatagtga attaagtgat
    31141 gctgtttact ggctagatcc taaacataga gactatgtac ctgaaatgca agaaaattta
    31201 agttttaaaa ttaacggcac tatgtataaa atcctaaaaa tcaaaaactc cttcgacggc
    31261 atgcaggcga tggctgtagc gccaatcgtc catagcaaat tggagaaaaa tttcaaaaat
    31321 aaaaaaattc ctgcaaactt tcgcgtactt aaataatgaa ggtgtcaata cttacataaa
    31381 aagaaaggag gcacgttctt gtcaaggaaa ataagaaaac tcagcttagc cattttatta
    31441 atcgtcatat tagggatcgg agggaaagtc tatatggaca aaagagaagc acaaaggcag
    31501 aaagatttac tcgcagtaga aaaacagtca gtcaaagttc tgaaaaatac ctttgcggat
    31561 atcagggaag tgaaggtaga agaatttaaa aaaaaccctg ttaccggaag ttatggtgca
    31621 cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt ttgattatag tttttggaag
    31681 gagagaaatg aacttggtgg ttttggcatt gagaacagag cggtacaaaa gacaggagtc
    31741 actataaaaa gagtaaaagt aattttttct aacggacagg aggagttagt atgacaataa
    31801 acgataagca ttacaatgat attagtgaga aggtttattg gctagatccc aaatatcccc
    31861 gctacaatga gggatacaaa aagaactctg ttaaagaatt tgctggtatg gaatttcaaa
    31921 tcctacaaat caaagactca ttggacggca tgcaggcgat gtctgtagcg ccaatcaaga
    31981 acggtaaagt ggatctttct caagtagtga ttgcttttgc aggaacgaac ttaagtgatt
    32041 ggaaagattt ggaaacggat gcaagaagta tcatgaatct ctacgggaat agaccaggag
    32101 caagtttttc tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag
    32161 cggggcaaat caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag
    32221 ctgctatttc tactaccggc cattcattag gtggattctt agcattatat gtagcagcag
    32281 agaaacaatg gaaaaatgtc ggcttcaacg ggccggaccc gtatgatctc ttatcaccag
    32341 aagcaaaaaa atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg
    32401 attttatcgg taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa
    32461 cagcattctt gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg
    32521 gaaagctgat tattcctgaa aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa
    32581 accaagcgac ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac
    32641 tacgattaag tggcggccgg ctgacagtta atgaacaaat ttacttagat gacacacaag
    32701 ctaggttagt agtggaaaag ttatcggcaa aaatgaaaag cgctatggaa gaagttatcc
    32761 gcatcaatca aagaggaatt tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag
    32821 cacgtgttgt agccccttcg ttgactgaac aggaaatcat agaagcatta gctagtgtgg
    32881 gcgccacaaa agaaaactta gtcgattctg cgaaagaagt ctatctgcta cgaatcaata
    32941 aggcaagacg tatgggggag gcctttgata cattggcgaa ggagatccaa gacaaaatca
    33001 atgaaatcgt ggcacgagat cgagaattag cacagcaatt caattaaagg agaagtagat
    33061 gaaactagaa gaattggaaa gagattatcg aagtgaacga agacaattag aagctcaaga
    33121 agatgaattg atccacttta agaatcaagg attacgattg atggagcaag aggagcagcg
    33181 actgcatcat gtattaaaga acttagcttt agataatgag cctctgtttc aagcaagacg
    33241 aagttattcg aattatcagg agcattatca acaagcccta caagtagaac gacgaaaact
    33301 ctatcagcag caagatgaac tagaacgttc ctatcgtcaa aaaatcagac aaataactat
    33361 ggagaaatca tcttatgaat gaatttttaa tgcaattact aaaagaatta gagaaaaatc
    33421 tacatcgatc aaatgtacgt tcagttggta tggggatatc tcagcaaatt tgggcagaaa
    33481 aacgattgac agatcaaaac taccaaatga ctgcagaaga agctgctcaa gaattttcta
    33541 accgacatgt acacttgaaa ggatcagtaa aagggcaatt tgcagaaaaa ctaactgaat
    33601 tattccaaag acaagaaaag aaattaaaac agtttcgtta gtaaagttat atagaaaaat
    33661 tttgtgaaaa tggttt
//
LOCUS       DACERL010000026        32082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.027, whole genome shotgun sequence.
ACCESSION   DACERL010000026 DACERL010000000
VERSION     DACERL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32082
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.027"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..189
                     /locus_tag="IXF54_001260"
     CDS             <1..189
                     /locus_tag="IXF54_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1404534.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IXF54_001261"
     CDS             complement(1055..1585)
                     /locus_tag="IXF54_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1404535.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IXF54_001262"
     CDS             complement(1582..2199)
                     /locus_tag="IXF54_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1404536.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IXF54_001263"
     CDS             complement(2781..3002)
                     /locus_tag="IXF54_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1404537.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IXF54_001264"
     CDS             complement(3180..4712)
                     /locus_tag="IXF54_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1404538.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IXF54_001265"
     CDS             complement(4848..5933)
                     /locus_tag="IXF54_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1404539.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IXF54_001266"
     CDS             complement(5948..6667)
                     /locus_tag="IXF54_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1404540.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IXF54_001267"
     CDS             complement(6664..7974)
                     /locus_tag="IXF54_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1404541.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IXF54_001268"
     CDS             complement(8001..8591)
                     /locus_tag="IXF54_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1404542.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IXF54_001269"
     CDS             complement(8909..9970)
                     /locus_tag="IXF54_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1404543.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IXF54_001270"
     CDS             complement(10538..11605)
                     /locus_tag="IXF54_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1404544.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IXF54_001271"
     CDS             complement(11625..12104)
                     /locus_tag="IXF54_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1404545.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IXF54_001272"
     CDS             complement(12121..12582)
                     /locus_tag="IXF54_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1404546.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IXF54_001273"
     CDS             complement(12596..13846)
                     /locus_tag="IXF54_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1404547.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IXF54_001274"
     CDS             complement(14224..15675)
                     /locus_tag="IXF54_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1404548.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IXF54_001275"
     CDS             complement(15996..16760)
                     /locus_tag="IXF54_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1404549.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IXF54_001276"
     CDS             complement(16788..17486)
                     /locus_tag="IXF54_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1404550.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IXF54_001277"
     CDS             complement(17498..18277)
                     /locus_tag="IXF54_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1404551.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IXF54_001278"
     CDS             complement(18293..19237)
                     /locus_tag="IXF54_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1404552.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IXF54_001279"
     CDS             complement(19283..20062)
                     /locus_tag="IXF54_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404553.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IXF54_001280"
     CDS             complement(20442..22517)
                     /locus_tag="IXF54_001280"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1404554.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXF54_001281"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXF54_001281"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1404555.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXF54_001282"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXF54_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1404556.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYFGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IXF54_001283"
     CDS             complement(24783..25682)
                     /locus_tag="IXF54_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1404557.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IXF54_001284"
     CDS             complement(25697..26098)
                     /locus_tag="IXF54_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1404558.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXF54_001285"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXF54_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1404559.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IXF54_001286"
     CDS             complement(26581..28773)
                     /locus_tag="IXF54_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1404560.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IXF54_001287"
     CDS             complement(28797..29768)
                     /locus_tag="IXF54_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1404561.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IXF54_001288"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IXF54_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1404562.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         9629 a         6556 c         4782 g        11115 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttaatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgaa ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gggctctata atatctagtg ttggtgacgc cttctgttgc
    32041 caatactttt tcttttttct aataaacgca aacagacacc tt
//
LOCUS       DACERL010000027        31702 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.028, whole genome shotgun sequence.
ACCESSION   DACERL010000027 DACERL010000000
VERSION     DACERL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31702
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.028"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(718..3867)
                     /locus_tag="IXF54_001289"
     CDS             complement(718..3867)
                     /locus_tag="IXF54_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1404563.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IXF54_001290"
     CDS             complement(3880..5079)
                     /locus_tag="IXF54_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1404564.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IXF54_001291"
     CDS             complement(5069..6664)
                     /locus_tag="IXF54_001291"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1404565.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7248..7955)
                     /locus_tag="IXF54_001292"
     CDS             complement(7248..7955)
                     /locus_tag="IXF54_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404566.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8020..8856)
                     /locus_tag="IXF54_001293"
     CDS             complement(8020..8856)
                     /locus_tag="IXF54_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1404567.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8874..10283)
                     /locus_tag="IXF54_001294"
     CDS             complement(8874..10283)
                     /locus_tag="IXF54_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1404568.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10297..12120)
                     /locus_tag="IXF54_001295"
     CDS             complement(10297..12120)
                     /locus_tag="IXF54_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404569.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12963..13289)
                     /locus_tag="IXF54_001296"
     CDS             complement(12963..13289)
                     /locus_tag="IXF54_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404570.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13335..13697)
                     /locus_tag="IXF54_001297"
     CDS             complement(13335..13697)
                     /locus_tag="IXF54_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1404571.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13734..13949)
                     /locus_tag="IXF54_001298"
     CDS             complement(13734..13949)
                     /locus_tag="IXF54_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1404572.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14076..14411
                     /locus_tag="IXF54_001299"
     CDS             14076..14411
                     /locus_tag="IXF54_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1404573.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14487..16484)
                     /locus_tag="IXF54_001300"
     CDS             complement(14487..16484)
                     /locus_tag="IXF54_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404574.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16582..16857)
                     /locus_tag="IXF54_001301"
     CDS             complement(16582..16857)
                     /locus_tag="IXF54_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404575.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17089..17823
                     /locus_tag="IXF54_001302"
     CDS             17089..17823
                     /locus_tag="IXF54_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1404576.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17862..19004
                     /locus_tag="IXF54_001303"
     CDS             17862..19004
                     /locus_tag="IXF54_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1404577.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IXF54_001304"
     CDS             complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IXF54_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1404578.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20495..20965)
                     /locus_tag="IXF54_001305"
     CDS             complement(20495..20965)
                     /locus_tag="IXF54_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1404579.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20952..22187)
                     /locus_tag="IXF54_001306"
     CDS             complement(20952..22187)
                     /locus_tag="IXF54_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1404580.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IXF54_001307"
     CDS             complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IXF54_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1404581.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IXF54_001308"
     CDS             complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IXF54_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1404582.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24453..25301)
                     /locus_tag="IXF54_001309"
     CDS             complement(24453..25301)
                     /locus_tag="IXF54_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404583.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25325..26011)
                     /locus_tag="IXF54_001310"
     CDS             complement(25325..26011)
                     /locus_tag="IXF54_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404584.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26004..27038)
                     /locus_tag="IXF54_001311"
     CDS             complement(26004..27038)
                     /locus_tag="IXF54_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404585.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27433..27771)
                     /locus_tag="IXF54_001312"
     CDS             complement(27433..27771)
                     /locus_tag="IXF54_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1404586.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27773..28129)
                     /locus_tag="IXF54_001313"
     CDS             complement(27773..28129)
                     /locus_tag="IXF54_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1404587.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28135..29322)
                     /locus_tag="IXF54_001314"
     CDS             complement(28135..29322)
                     /locus_tag="IXF54_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1404588.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29513..30358)
                     /locus_tag="IXF54_001315"
     CDS             complement(29513..30358)
                     /locus_tag="IXF54_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1404589.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30592..31557
                     /locus_tag="IXF54_001316"
     CDS             30592..31557
                     /locus_tag="IXF54_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1404590.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9183 a         6632 c         5334 g        10553 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     6961 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     7021 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     7081 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     7141 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     7201 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
     7261 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
     7321 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     7381 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
     7441 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
     7501 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
     7561 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
     7621 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
     7681 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
     7741 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
     7801 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
     7861 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
     7921 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
     7981 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     8041 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     8101 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     8161 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     8221 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     8281 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     8341 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     8401 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     8461 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     8521 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     8581 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     8641 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     8701 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     8761 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     8821 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     8881 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     8941 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     9001 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     9061 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     9121 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     9181 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     9241 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     9301 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     9361 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     9421 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     9481 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     9541 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     9601 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     9661 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     9721 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     9781 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     9841 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     9901 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     9961 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
    10021 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
    10081 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
    10141 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
    10201 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
    10261 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
    10321 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
    10381 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
    10441 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
    10501 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
    10561 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
    10621 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
    10681 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
    10741 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
    10801 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
    10861 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
    10921 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
    10981 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
    11041 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
    11101 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
    11161 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
    11221 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
    11281 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
    11341 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
    11401 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
    11461 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
    11521 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
    11581 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
    11641 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
    11701 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
    11761 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
    11821 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
    11881 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
    11941 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
    12001 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
    12061 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
    12121 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
    12181 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
    12241 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
    12301 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
    12361 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
    12421 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
    12481 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
    12541 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
    12601 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
    12661 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
    12721 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
    12781 tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca
    12841 atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa
    12901 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
    12961 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
    13021 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
    13081 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
    13141 tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta
    13201 cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca
    13261 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgt
    13321 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
    13381 acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc
    13441 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa
    13501 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
    13561 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
    13621 gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca
    13681 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg
    13741 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
    13801 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
    13861 tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg
    13921 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
    13981 aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac
    14041 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
    14101 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
    14161 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
    14221 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
    14281 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
    14341 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
    14401 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
    14461 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
    14521 actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct
    14581 cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt
    14641 aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
    14701 agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg
    14761 ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt
    14821 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
    14881 tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
    14941 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt
    15001 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
    15061 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
    15121 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
    15181 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
    15241 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
    15301 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
    15361 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
    15421 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
    15481 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
    15541 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
    15601 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
    15661 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
    15721 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
    15781 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
    15841 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
    15901 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
    15961 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
    16021 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
    16081 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
    16141 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
    16201 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
    16261 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
    16321 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
    16381 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
    16441 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
    16501 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
    16561 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
    16621 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
    16681 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
    16741 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
    16801 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
    16861 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
    16921 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
    16981 tcgacataca cataatacct cgataaaaaa ggggtgtcaa tagttttgta aaatttattt
    17041 acaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
    17101 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
    17161 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
    17221 gacaaaaaga ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
    17281 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
    17341 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
    17401 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
    17461 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
    17521 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
    17581 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
    17641 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
    17701 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
    17761 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
    17821 tagtggtaag tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt
    17881 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
    17941 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
    18001 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
    18061 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
    18121 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
    18181 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
    18241 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
    18301 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
    18361 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
    18421 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
    18481 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
    18541 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
    18601 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
    18661 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
    18721 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
    18781 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
    18841 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
    18901 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
    18961 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
    19021 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt
    19081 ctagctccta taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt
    19141 gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac
    19201 gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc
    19261 ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc
    19321 attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat
    19381 atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc
    19441 tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg
    19501 gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat
    19561 ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc
    19621 acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt
    19681 tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc
    19741 ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc
    19801 cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc
    19861 gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt
    19921 gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc
    19981 taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta
    20041 ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt
    20101 catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg
    20161 ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc
    20221 ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat
    20281 gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt
    20341 cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac
    20401 ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa
    20461 tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat
    20521 ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg
    20581 cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt
    20641 ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt
    20701 ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac
    20761 tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga
    20821 cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt
    20881 cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata
    20941 aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca
    21001 actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct
    21061 gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc
    21121 ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca
    21181 acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct
    21241 tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata
    21301 ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt
    21361 gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa
    21421 tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca
    21481 tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca
    21541 cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg
    21601 atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct
    21661 aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct
    21721 gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct
    21781 gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc
    21841 actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag
    21901 cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc
    21961 cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca
    22021 ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt
    22081 tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct
    22141 tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc
    22201 cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag
    22261 aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca
    22321 tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta
    22381 cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca
    22441 tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga
    22501 taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga
    22561 tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc
    22621 tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt
    22681 cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta
    22741 aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga tctacggctg
    22801 aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt
    22861 ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat
    22921 ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata
    22981 aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt
    23041 tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca
    23101 tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa
    23161 tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt
    23221 ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga
    23281 tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat
    23341 gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat
    23401 caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt
    23461 tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt
    23521 tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca
    23581 tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc
    23641 gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt
    23701 tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa
    23761 atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa
    23821 aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt
    23881 catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat
    23941 tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca
    24001 tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc
    24061 cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag
    24121 ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca
    24181 ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta
    24241 tttctaagac agacatgcat tataccccca aattctcttt cgttctcact cattttagac
    24301 taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat
    24361 taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac
    24421 agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta
    24481 acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca
    24541 atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg
    24601 taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg
    24661 taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct
    24721 aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca
    24781 tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt
    24841 agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca
    24901 tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg
    24961 acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga
    25021 atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca
    25081 tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca
    25141 tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct
    25201 gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc
    25261 agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat
    25321 cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata
    25381 cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc
    25441 cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa
    25501 atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag
    25561 gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca
    25621 gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg
    25681 cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt
    25741 tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac
    25801 tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag
    25861 agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg
    25921 tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat
    25981 ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt
    26041 tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc
    26101 aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg
    26161 atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag
    26221 gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc
    26281 gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt
    26341 tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg
    26401 actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa
    26461 ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc
    26521 aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg
    26581 ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct
    26641 tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg
    26701 ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg
    26761 aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat
    26821 tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc
    26881 aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc
    26941 cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg
    27001 aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat
    27061 aatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa
    27121 cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg
    27181 gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct
    27241 tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag
    27301 caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt
    27361 tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt
    27421 gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat
    27481 ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt
    27541 aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc
    27601 aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa
    27661 cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc
    27721 attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca
    27781 gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga
    27841 tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg
    27901 taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga
    27961 acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg
    28021 gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt
    28081 tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct
    28141 ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc
    28201 gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt
    28261 taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat
    28321 gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat
    28381 catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa
    28441 gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct
    28501 tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg
    28561 ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa
    28621 ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa
    28681 gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca
    28741 agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc
    28801 aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc
    28861 aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac
    28921 gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa
    28981 cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa
    29041 tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca
    29101 gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca
    29161 gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc
    29221 tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata
    29281 atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta
    29341 aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga
    29401 catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc
    29461 ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc
    29521 tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg
    29581 caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat
    29641 ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc
    29701 gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct
    29761 tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg
    29821 cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca
    29881 tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg
    29941 attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat
    30001 gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa
    30061 aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt
    30121 gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt
    30181 gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc
    30241 gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg
    30301 ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg
    30361 caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa
    30421 aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga
    30481 tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta
    30541 ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac
    30601 tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga
    30661 aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg
    30721 attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg
    30781 ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt
    30841 tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc
    30901 gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct
    30961 gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc
    31021 atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca
    31081 ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat
    31141 atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta
    31201 acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct
    31261 ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt
    31321 ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt
    31381 actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa
    31441 gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt
    31501 ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa
    31561 tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa
    31621 aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac
    31681 accgtttacc aagaggtcgt aa
//
LOCUS       DACERL010000028        30932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.029, whole genome shotgun sequence.
ACCESSION   DACERL010000028 DACERL010000000
VERSION     DACERL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30932
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.029"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(133..498)
                     /locus_tag="IXF54_001317"
     CDS             complement(133..498)
                     /locus_tag="IXF54_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404591.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            706..1188
                     /locus_tag="IXF54_001318"
     CDS             706..1188
                     /locus_tag="IXF54_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404592.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1385..2275
                     /gene="pstS"
                     /locus_tag="IXF54_001319"
     CDS             1385..2275
                     /gene="pstS"
                     /locus_tag="IXF54_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1404593.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2600..5071
                     /locus_tag="IXF54_001320"
     CDS             2600..5071
                     /locus_tag="IXF54_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1404594.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5174..6910)
                     /locus_tag="IXF54_001321"
     CDS             complement(5174..6910)
                     /locus_tag="IXF54_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1404595.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6907..7611)
                     /locus_tag="IXF54_001322"
     CDS             complement(6907..7611)
                     /locus_tag="IXF54_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1404596.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7742..8245
                     /locus_tag="IXF54_001323"
     CDS             7742..8245
                     /locus_tag="IXF54_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1404597.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8205..8543
                     /locus_tag="IXF54_001324"
     CDS             8205..8543
                     /locus_tag="IXF54_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1404598.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8564..9982
                     /gene="ffh"
                     /locus_tag="IXF54_001325"
     CDS             8564..9982
                     /gene="ffh"
                     /locus_tag="IXF54_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1404599.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10094..10672
                     /locus_tag="IXF54_001326"
     CDS             10094..10672
                     /locus_tag="IXF54_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1404600.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10676..11197
                     /locus_tag="IXF54_001327"
     CDS             10676..11197
                     /locus_tag="IXF54_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1404601.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11276..11830)
                     /locus_tag="IXF54_001328"
     CDS             complement(11276..11830)
                     /locus_tag="IXF54_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1404602.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12083..12358
                     /gene="rpsP"
                     /locus_tag="IXF54_001329"
     CDS             12083..12358
                     /gene="rpsP"
                     /locus_tag="IXF54_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1404603.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12370..12621
                     /locus_tag="IXF54_001330"
     CDS             12370..12621
                     /locus_tag="IXF54_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1404604.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12746..13537
                     /locus_tag="IXF54_001331"
     CDS             12746..13537
                     /locus_tag="IXF54_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404605.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13707..14228
                     /gene="rimM"
                     /locus_tag="IXF54_001332"
     CDS             13707..14228
                     /gene="rimM"
                     /locus_tag="IXF54_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1404606.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14218..14979
                     /gene="trmD"
                     /locus_tag="IXF54_001333"
     CDS             14218..14979
                     /gene="trmD"
                     /locus_tag="IXF54_001333"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1404607.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15115..15462
                     /gene="rplS"
                     /locus_tag="IXF54_001334"
     CDS             15115..15462
                     /gene="rplS"
                     /locus_tag="IXF54_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1404608.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15835..16497
                     /locus_tag="IXF54_001335"
     CDS             15835..16497
                     /locus_tag="IXF54_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404609.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            16576..19803
                     /gene="fss3"
                     /locus_tag="IXF54_001336"
     CDS             16576..19803
                     /gene="fss3"
                     /locus_tag="IXF54_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1404610.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            19917..20231
                     /locus_tag="IXF54_001337"
     CDS             19917..20231
                     /locus_tag="IXF54_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1404611.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20244..20618
                     /locus_tag="IXF54_001338"
     CDS             20244..20618
                     /locus_tag="IXF54_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1404612.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            20628..20972
                     /locus_tag="IXF54_001339"
     CDS             20628..20972
                     /locus_tag="IXF54_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1404613.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21043..22395
                     /locus_tag="IXF54_001340"
     CDS             21043..22395
                     /locus_tag="IXF54_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1404614.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22455..23597
                     /locus_tag="IXF54_001341"
     CDS             22455..23597
                     /locus_tag="IXF54_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1404615.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            23622..23876
                     /locus_tag="IXF54_001342"
     CDS             23622..23876
                     /locus_tag="IXF54_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404616.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24132..25316
                     /locus_tag="IXF54_001343"
     CDS             24132..25316
                     /locus_tag="IXF54_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1404617.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25331..25450
                     /locus_tag="IXF54_001344"
     CDS             25331..25450
                     /locus_tag="IXF54_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1404618.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            25455..25676
                     /locus_tag="IXF54_001345"
     CDS             25455..25676
                     /locus_tag="IXF54_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404619.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            25689..26189
                     /locus_tag="IXF54_001346"
     CDS             25689..26189
                     /locus_tag="IXF54_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1404620.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            26286..28133
                     /locus_tag="IXF54_001347"
     CDS             26286..28133
                     /locus_tag="IXF54_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1404621.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            28136..29032
                     /locus_tag="IXF54_001348"
     CDS             28136..29032
                     /locus_tag="IXF54_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1404622.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            29082..29471
                     /locus_tag="IXF54_001349"
     CDS             29082..29471
                     /locus_tag="IXF54_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1404623.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            29458..>30932
                     /locus_tag="IXF54_001350"
     CDS             29458..>30932
                     /locus_tag="IXF54_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1404624.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        10366 a         5282 c         6598 g         8686 t
ORIGIN      
        1 aaatgctgat atttcagcat tttttaagag tataaaagag aaaaaaagta ataaatgtat
       61 acatgatata agaaaaacat atatgtataa aaagaagaaa gtagcttctc tttgtacata
      121 tatggtttgt ttttatggat gccgagcttt tttataagat aaggctaccg cccataacga
      181 cccaaggatt tgtggaacta agtaaatacc tgcaagaagc aagatcagct gggtcggttt
      241 taaaggatga tagaagtaac gccattcagt aaaattggcg caaaataaat aggaagtgac
      301 agtagatata atattggcaa tgaataaaga tataatacgc ctgttccttt gaaaataata
      361 tccagcaaaa agagataaaa atatcaatgt gatgaacccg cttacggaat gataacgaaa
      421 atcaagcagt aaggcgaaat gcacataagg aacaaaagat aaaagcaacg gaaggttttt
      481 ccagcaagtt tttatcatct ttcatcacca cctaacaagt gttattattt tgttagtttt
      541 attgtaaatt gaatcctgca ataatcaatt actttttcat tatttctaga ttaaatgaat
      601 aaactctcat gccattatct ctaaaaaaaa agtttttccc cctaattttt ttagatagaa
      661 aattgtatag tatgatgaga gaaaaagtag tttgggggaa cgttaatgag aagaaaagca
      721 gtaatcgatc ggctttattt atttttttgg ttttttccta tcgttttgct accttttaca
      781 tgggtaaatg ctgttgtaca aggtgcggag atattaggcc tgatccaagg aagcggattt
      841 gtggtttcta ggaattttcc agctttgact gttttctatt tagtgataag tgtaggtctc
      901 ttattaccaa aatctctgat caatcactac aattggcagc tgctttactt ttcttgctta
      961 ttagtattta catctatgtt ttctttgctg cctcgcttga ttatcggccc taatggtttg
     1021 agcgagagtg gattgaagga ttcagcttat tcctccatgt atttcttgac gatcaaacca
     1081 tggttctatt tagtaattgc ctcatttatt cttgcaacat ttttttactt ctggacagaa
     1141 atcaggataa aaagtgaacg atctgtatct ataaaaaata actattgatg gtggatcatc
     1201 agtaaaaaag catatgagct gcgtaaaatg catgtttctc atatgctttt tttattatta
     1261 tgtaaagaat gcgaaaaacg tttacatgat ttttacataa cttaactata tttttacagc
     1321 aaccatacga gggatttaca gtcgactgat aaactttagt tgtaaataag aaaggagcaa
     1381 taaaatgaaa aaagtattgt ttactctagg tatgatggga attcttttag ccggatgcgg
     1441 ctcaggatca ggaaatacag atggctctgc cacacaaaat aattcaagtg actcaaatga
     1501 acaagtaaaa atcgttgcgg taggttcgac agcattacag ccgttagtcg atgcagcaca
     1561 agaaagcttt gtacaagaaa atccaaatta tcaaatctct gtacaaggag gaggaagcgg
     1621 aactggtttg agccaagtcg aagctggagc agttactatc ggaaactctg atgtatttgc
     1681 agaagaaaga gacggagttg atgcttcaaa attagtggat cataaagtag cagtggtcgg
     1741 gatggcgcca atcgtcaata aagatacaga tgtcaaagat atcaccaaac aggaattgat
     1801 tgatatcttt acaggaaaaa tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat
     1861 caacgtcgtg aaccgtgcaa acggcagcgg tacccgcgcg acattcgaaa aatggggact
     1921 ggatggtgct acccctgtgc agtcccaaga acaagattca tcaggaacag ttcggcagct
     1981 ggtaagtcaa acaccaggag caatcagcta tttagctttc tcatatctcg atgattccac
     2041 acaagcacta agtatcgacg gtgtagaacc aaaagaagag aatgtagcag ataacagctg
     2101 gggaatctgg tcatatgaac atatgtacac gaacggaaaa ccttcccctg aagtccaaaa
     2161 attcttagac tacatgatga cagaagaaat ccaagaagga cctgtcaaag aattgggcta
     2221 tttaccaatc acaatgatgg aagtagaacg tgatcacgaa ggaaatatca aataactagc
     2281 agataaacat gccacggtag tcgttactat taaggaaaaa tcaactatgt ttcccacata
     2341 tacatttttc ctttaattgt gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     2401 accgagggtc agctgattaa acaccgttta ttcgaaaaaa agagctacga catagaactg
     2461 tcgtagctct ttttttgcta tatgacagga atgaaaggtg tttgcaagct taatgtgagg
     2521 aacactttcc atatctgttc tatactggat aggagaagtt tggaaatatc cagaaaacga
     2581 aatcaatcga ggtagccaca tgaagaataa atggataaat cgaagtaaaa aaaagaaagt
     2641 aacacaaaga tcatcagata aagtcatccg tctttttcca tcgtataaag aaggattaac
     2701 ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca aataattcag tcgatcccac
     2761 attcaaaaca aatcagcaaa tcgtattgga aaacatattt acgtatttca atttgatctt
     2821 tcttatctta gccattttgc tttgtttagt agaatcctat aagaacttga cttttttacc
     2881 agttatcata gccaatacag gaatcgccat ttttcaggaa attcgatcca aaaagatctt
     2941 ggacgaacta aatgttttaa atacagcaaa agtcaatgtc atacgggacg ggatagaaca
     3001 aacaatagat atagaagacc ttgtaataga tgacattgtt attttagaga ctggacatca
     3061 aattccagca gatgcagagg tcgtggaagg aaaactgcaa gttaatgagg ctttattgac
     3121 aggcgaagca gatgaaataa ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat
     3181 tgtttccgga aaagcttacg cccgattaga caaagttgga gcagattcgt atatttccca
     3241 gctgacagtc aaagccaaga caatgggaga aggagaacag tcggagatga ttgcttctct
     3301 taatcaattg atcaaatgga ttgggatcat cattattcct atcggtatcc tgcttttttc
     3361 tcaaagttac ttttttaatg gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc
     3421 attgatcggt atgatacctg aaggactgta cttgctgacg acgattgcat tagcattaag
     3481 tgcgactaaa ctagcaaagc aaagagttct tttacacaat atgaaaagca ttgagacatt
     3541 ggcaagggta aatgtccttt gtgtagataa aacaggaacg atcacagaaa ataaaatgtc
     3601 tgttcaaaaa gtgatcgtct ctaaaaaaca agaaataatc agtcagtcag atcaattgga
     3661 agcatgcatt gctgactatg caaaagcgat ggcaggagac aatgcaacaa tggaagctgt
     3721 taaagcatat tttacaaaaa ccactgatca atcctatcac catgttattc ctttttcttc
     3781 tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg tatgttttag gagcgcccga
     3841 aatggttttg cgcgatcaat tgccggaata tgcggaagag tttgtttctt tcgctgaaaa
     3901 aggctatcgc gtgttggtct ttggtgtgta cgatgggatt cttgaagaac cagtattgac
     3961 agaagcagtc acaccattag gctatattct gattgctaat cctatcagaa aagaagcaag
     4021 agccacattt gattacttca aaaaacagca agtagccatc aaagtgattt ctggagataa
     4081 tccgctcacc gtatccaatg tagctgtcca agcagggatt tcacatgctg atcagtacgt
     4141 tgatgcatct aaattatcag aagaagaata tgaagcagct gtcgaaaaat atacagtttt
     4201 tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac
     4261 agtagctatg acaggtgatg gcgtgaatga tattctagcc atgaaagaag cagactgcag
     4321 tatagcgatg gcttccggaa atgaagccgc catgcaggcc tcacaagtgg tcttacttga
     4381 ttctgacttt tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg tgaacaatat
     4441 tgagcgatca gccagcttgt ttttagtgaa gaatattttc tcctttttgc tctcactgtt
     4501 ttcagtgatc tttgctctga cctatccttt agaaccatca caaatcacat tgattagttt
     4561 gtttactatc ggacttccat ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag
     4621 agggaaattc atcatgaatg tgatggaaaa agctgtgcca ggtggactaa cagatatgat
     4681 cgtagtcggt gcgttggtga tctgcggggt gactttgaat ctaaataaaa cagatgtttc
     4741 tacagcatct accatgcttt tgattgcagt agggttcctc gttctctata aaatctgttc
     4801 accgctgaat aaattcagaa gtcagatcat tcttttctgt gctagcggta actttttttc
     4861 ggtcatcttt ttgcataagc tgttttcaat caccggtatc tcagctgtat cacttttgct
     4921 gttgagcatt ctatttttct cggcagattc gatttttagg catttgacca cattggtaga
     4981 atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa
     5041 atttctatcg atatttaaac gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa
     5101 gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa aacgcacgag
     5161 cagttgctct tttttacatt ttcggaatgg taacggtgaa agtcgaacct gttccaagat
     5221 gactgtctac agtaatcgta ccattaagga gttgcacgta gtcttttaca atcgctaacc
     5281 ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa
     5341 agatccgttc ctgatcctca atgtcgatac ctataccaaa gtcttggaca gaaaacgata
     5401 gatcatctgt tgcctgatag cggataataa tctcgctgtc gctttttgaa tattggacag
     5461 cattttcaat cagatttttg ataattggat aaaggatatc tgttttagta gtaaatggtt
     5521 cgttctttcc aatcaatcga atcgtcaacc gtttcttttc gatgattggc tgataatttt
     5581 gaacgatttg ctggaaataa ggttctgtat aaattgtctg gatctcgtaa gaattctcgc
     5641 catcttttga cagctggatg atttcccgaa tcagtttatc taaccggatc gcatcttttt
     5701 gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca tccagtaaag
     5761 tctcggtaaa accaatcagt gaagtcacag gagtttttaa ttcgtgagag acgttgccaa
     5821 cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt taagtcatac gccaccccta
     5881 gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa actcaagtct aatgtctgcc
     5941 gaccttctgt aagggtgatc tcctgatgga tcagcggatg ctcaggagtc acttgatgaa
     6001 tcagctgaat gagttttggc tcttgaatga cttctgtgta ccgctgttca ggttgatagc
     6061 ttaccacgtt taaatgtagc tgcatctttg gattaagcag tagtaaacga gaatcagcat
     6121 catcaatcag gaatacgcca atcatcagtt cgttcagtaa cgtatataat tgctcttctg
     6181 tggtagtaca cgcccgatac atcttactca tttgttcgct caacgcatta atcgtctgat
     6241 acaattcttc ccattgatcg ggtgtttgca tgattacttc cgcctgatta ggattttgca
     6301 ccattttttt tagtacgggc agcacggttt tcaatggctg gtttttttga tagatcagat
     6361 aatagatcat tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag acccaacggc
     6421 gaaaactctc agttctcggt aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt
     6481 ctaactggcc actttttttg actggtaatg cgacatataa aagatcctca ttcaaagtag
     6541 tacttttgcg caaagcgcta cctaggttcc caccattcaa tacggctttg atttcgggac
     6601 gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc aaataagatc tttccagtag
     6661 catccattaa agtaatccgt tcgttcgact gatggacgaa ttcttccaaa gcagttttgt
     6721 tttgcgctgc ttggaggttt tcgacatcta attgccggat cagcagttgt gctttttttg
     6781 tcaggtagct ttcttgctgt tccacgatct gctgttgaaa ataatgggaa atcaattgcc
     6841 agccaccaaa aaacaaacta actaaaagaa taagaaagaa gagacgttca taaatttttt
     6901 tccatttcat cgttttggtt cctgaaactt atagccaaat cctctaatag tcaccaagta
     6961 ctttggatgt tttggatctc gctcgatttt ttctcgcaaa tgactaacgt gtacatctac
     7021 gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc
     7081 gatcacacgg tcttttcgtt tcatgaaata aacaagtaat tcaaattctt tcggtgttag
     7141 ttcaatcaga tggtcgtcta cggtcacttg ataattggtc aaatcagcag tgatttgtcc
     7201 aatcgataga tattcaggct cagcttcatc ttttgattgc ttacgaggtt caatccgtct
     7261 gaatattgct ttcatacggg ctaatacttc gcgaggacta aatggtttgg tcaaatagtc
     7321 gtcagcacct atttctaggc caatgatccg atccacttgg tcatctttag ccgtcagcat
     7381 caaaatcggt gtttcatttt tttcttgacg cagccgctga gtgatctcca tgccatccat
     7441 cgttggaagc atcacatcta aaatgatgaa atcaaatgat tgttctaatg ctaattctaa
     7501 tccttcgcct ccatctgtgg cactggttac cttgtagcct tctttttcca aattgaacgt
     7561 caagagcgtt acaatagaag gttcatcatc aacaactagt atttttttca ttcaaatctc
     7621 ctttaaatag gtttcattct gtttatattt tagcatagaa tcagtacaaa gggctatttt
     7681 acaaaagaaa taaatggtat aataggaagg cactttgctg aatagaaagg aagagcagtc
     7741 aatgattgga atcagtgcat gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa
     7801 agaagtacca gaattgatgg aactagttga aactggagaa gctgtcatgg tctgtccaga
     7861 agtgatcggc ggattaccaa ttcctagaga tcccgctgaa atcattggtg gaaatggatt
     7921 cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc ggcgaagatg taacggaagc
     7981 gtacaaaaat ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt
     8041 catcatgaag gaaaaaagcc cttcatgcgg aacccattcg atttatgacg gaagtttttc
     8101 cggaataaaa aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac
     8161 tgtgctctct gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga
     8221 ccaatcgaat gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga
     8281 actacatgga attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg
     8341 aagtaagccg acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt
     8401 atgaaaaaaa actccatctt ttttctaatt atattgttcg agaacaggta ttggaaaata
     8461 tgactgcata tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc
     8521 aaattcaagc aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt
     8581 aactgaacgt ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc
     8641 tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt
     8701 gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct
     8761 ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac
     8821 acttggttct gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat
     8881 ggctggtctt caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa
     8941 gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat
     9001 cgaccaattg aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga
     9061 agtcagcccg gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga
     9121 ctatgtattg atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt
     9181 gaaacaaatc aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac
     9241 tggtcaagat gccgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt
     9301 cgtcatcact aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt
     9361 gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt
     9421 ccacccagat cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga
     9481 aaaagcgcag caagactacg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga
     9541 aaattcattc gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc
     9601 actggaagat ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt
     9661 caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc
     9721 agaaagagca aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc
     9781 tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat
     9841 gatgcagcaa atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc
     9901 aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa
     9961 gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct
    10021 actgccggag cttttctatc agcaattttt attctttact aatacaatta ctctaaacga
    10081 agaggtggca cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca
    10141 tgatactgtc tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct
    10201 tgatattaat caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga
    10261 tgaagcatat gatcgattcg atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag
    10321 tgaactaatg gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca
    10381 aaacgccaaa gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg
    10441 gtcgttttct aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac
    10501 aacaagtcct ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat
    10561 cggaagcaca acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga
    10621 cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg
    10681 aaaaatcata ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga
    10741 tcttcaatgg ctttttgaca ctccaacagg taagcaaact acttatgaag ctttttatga
    10801 aacaatcgat acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga
    10861 gaccgaagca atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca
    10921 attgccagat gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat
    10981 gacagaagga aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga
    11041 acatcaaatg attgatgaat ggtatatcca gattacccct gtattattag gggacgggat
    11101 tcctttattc caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt
    11161 cggtgaattt atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca
    11221 actatatgca gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact
    11281 ctgttaattt gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg
    11341 ttccttcaac gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt
    11401 ccggttgctg caaggttggc acattcaaaa agacaagtga ttggcgtaga tggtggtttg
    11461 cgccaaagcc gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc
    11521 aaacactgtg tccataaggt cttgagccta catctaaagc atttttcaat actgctggca
    11581 cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac
    11641 gaaaacgagt ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg
    11701 gcagatcatc gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag
    11761 cttctgctac ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt
    11821 tttttgtcat acgatttttc ctccttcaaa ctatctataa taatcttaca aaagataagt
    11881 aactggagca aataaaaaca cttactgaca aaagctacat agaagataga ttacttattt
    11941 ttaacaaaaa agaagtctaa aatcttctat tttctaaaaa aaatcgaatt taatcaagct
    12001 gtaaagaaaa aaagctttac agggtaaaaa aaaactgtta cactacatct tgtgaataaa
    12061 acaatggagg tggaaataaa taatggcagt taaaatccgt ttaaaacgta tgggttctaa
    12121 aaagagtccg ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt
    12181 catcgaaact gtaggtactt acaatccttt gaaagaccct gcagaagtag ttttaaaaga
    12241 agacttagtt cttgactggt tatcaaaagg tgcacaacct tctgacactg tacgtaacat
    12301 cctttcaaaa gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa agaaataagg
    12361 tgaccggata tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc gctagtcact
    12421 tatcctgatc aagtcaagtt agacgtcgta gagtcgaaag acttttacga gtacaatcta
    12481 acagtggctc ctgaagatat tggccgtatc attggaaaac aagggcgtgt tgctaaagca
    12541 atccgcacaa tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc
    12601 atcgatgata aggaaaagta aataaacgtg ctgtactcga attttcgagt gcagcttttt
    12661 tgtttttttt attaattgaa gaaatgatta tttggcaaaa caagcaagaa agcgtatcct
    12721 tataggagga gggagtgaac agccaatgag aaagaaaatg ttatttattc catttgctct
    12781 attgttaagc gcttgtagtt cggttactca agaatcagac aatacacatc tatcaagcag
    12841 tatatctgaa actgttacca gtccagaaaa aagtagtacc aaaacggctt ccaccactga
    12901 acaaacaatg gctacctcaa ataatgagaa aaagactgct ttagaccagt tgaaagaaca
    12961 acagccaaat gttcccatgc ctctagatgt accagtctct tcaggctatc tcaatatcgc
    13021 agcgacacat acaaagcaag gttattctat tttatattac agaacagatc gtcccctcgg
    13081 gttgaatgct gacgaattga atcaagaaac gcctattgca acctatctat atcaatatgg
    13141 gtttgcttct agtcaagaaa caatccaagt tttgcagcca tttgagatcg acacaaatgg
    13201 tcagcaagtg gacttaggtt ctcgaatcac agggtatcag caaggtgcag caggttcaag
    13261 ttttcttgaa tggcaagaag gaaactggcg tattcgaatc cgtgggaata atatagaagg
    13321 ccaagatccc ttactattag ctaaagaaat cgtagcttat ttggaagaaa acagtctacc
    13381 agcacctgaa caatttggta aaattactgt tgatatgggt gatacgacga atcgagccgt
    13441 agaagtaagc tggcaagaac cgaaaaatgc ctatacgatc actcatcaag atcctatgtc
    13501 tgcgttgaag atggcagttt ctatgaagag gttgtgaaat cagcgcgctg atctgagccc
    13561 atcatttaac tccaagtcac aggaagtaat caaaaatagc acgccgcatt tttgcataat
    13621 tttggcttaa taccttaaaa gaaaactaga attgcctctt cacacatgct aaaataaaac
    13681 agacaaaaaa gaaatgggga tataagttga ctgaatactt gaatgtagga aaaatcgtaa
    13741 acacacaagg tatcaaaggc gaagtccgtg ttatctcaac aacggatttc ccagaagaac
    13801 gttacaaaaa aggaacggtg ctaacactat tccaagacgg aaaagcacct gtggaattaa
    13861 cagtcaaaag tcatcgcaaa cataaaaatt ttgacttatt gagttttgaa ggacatcctt
    13921 ctatcaatga tgtggaaaaa tatcgggatg gcatattaaa agtgtcaaaa gacaactctg
    13981 tagatctagc tgaaaatgaa ttctattacc atgagatcat cggtctgcat gtattagaag
    14041 atggaactga gttaggtaaa gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg
    14101 tcgttcaacg tccgaaaaaa gctgatatat tgcttccata tatcgcttct gtcgtcaaag
    14161 aagtcgattt agaagctgga gtggtccgtg tagaaattcc agaaggactg attgacgatg
    14221 aagattgatg tattgacttt atttccaaga atgtttgaag gtcctatggg ggaatccatc
    14281 atcggtaagg ctgtagataa aggattatta gatatcaata tctctaactt ccgcgattat
    14341 tcagataaca aacatcagac agtagatgat tatccatacg gcggtggagc aggtatgctg
    14401 ctcaaagtcc agcccatcta tgataacatc caagccattg aaaaagctgc accggatgtc
    14461 aaaaaaagag taattcttct tgacccagct ggtaaacgat tcgatcagaa aatggcagaa
    14521 gaattttcac aagaagaaca cttagtattc atttgtggtc attatgaggg atatgatgaa
    14581 cgtatccgca cattagtgac agatgaagtt tccttaggag attatgtcct tactggggga
    14641 gaattaggtg cgatggtcat gatcgatgca acagttcggc ttttgccaga tgtgttaggg
    14701 aatcagactt cagctcaaac ggattcttat tcaaccggtt tattggaaca tccccaatat
    14761 actcgcccgg cagaatataa agggatgaaa gtaccagaag tattgacaaa tggaaatcat
    14821 aaattgattg aagaatggca attaaaagaa tcactaagaa ggacctatca acgtcggcct
    14881 gatcttttgg aaacacttga atggacacct caaatgacga aatttttaga agaaatcaag
    14941 aaagaagaac aagaaaaaac agcagatgaa gccaagtgac tttttctaaa atcttcttta
    15001 cagtaggttg ataatatgtt agtattttat ggtgagtgca aaagcactta actattacga
    15061 tattccgctg tgagaaaagc tcataagaat atttggaata aggagaagaa aaccatgaat
    15121 ccattaatcg aagaattaac aaaagaacaa ttacgttctg acatcccagc gttccgccct
    15181 ggtgacactg tgcgtgttca cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta
    15241 tttgaaggtg ttgttatcaa acgccgcggt gctggaatca gcgaaactta tacagtacgt
    15301 aaagtttcta acggagtggg cgttgaacgt acattcccat tgcatacacc acgtgttgca
    15361 aaaatcgaag tagttcgcta cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca
    15421 ttacacggaa aagctgctcg tatcaaagaa atcagaagat aaacacggaa aacttccttg
    15481 gaaatctgaa atttgtgaaa agtccttgta tatcaagggc tttttctttt ttctctatca
    15541 cagttttaag ccatttttgg ctgttttgga ccatttttca atcatattcc cttaaaaatt
    15601 gtcctagatt gtccgacatt gactatcagt gtcgtcaaaa tagggcaaaa tttagtagtc
    15661 agcttgacga caaatataat acgaaaaata ccaaaacatt taccgaattt agcaactttt
    15721 gaacccaagt ttctcaaagg gtcagctatt agcaaaaaag acaaaacatg tacttttcta
    15781 ctaaaaagtt gatgtgatag gcttctatct aaataactat taggagtttt tatcgtgaag
    15841 caaaatgatg gacgtatcat ttggaaaaat caaagtgagt taaaactgat tttaacaatt
    15901 aatgaattca tcgaaaaaca cggaattaca tctagccgtc aataccagaa aaagctatca
    15961 gaaaatccaa attctgcacc aagtatgtgg ttcattaaca aaaaatatgg atcatgggag
    16021 aatttattga ttagtatagg acgagaaaat actgggtatg gaaaatgggc tagaatgtca
    16081 gaacaagaat tattggaaat agtagaagcc tttattaagt gcgagaagat aacctctcaa
    16141 cgaatgtacg aacaaaaatc agtagggaag aatattcctt cacttagtac cattaaaaag
    16201 atgttaggag atatacgtcc actttttaaa gaaaaaaatg atggatctcg atttactgat
    16261 ttcgaactgc tgttagaact aaaaaatgaa ataatccgat taaaactaca ggatgattta
    16321 tcaatgacaa agtttcgaaa actagttcaa tccccaaaac taccatcagt tgatacaatt
    16381 atgaaaagaa cgaacaaaaa ttgggaggaa ttgatggcag agattggatt tgattatcga
    16441 agaatcaaaa tttataagca gagaaacaac ctatccaaga caaagaaaac taaatagtat
    16501 cactctaaat cagacgttca aaattaaaat ttggacgtct tttttattac ccaaaaacga
    16561 aaggagattt attttatgaa aaagccaaga tttaagaatt ggcgtttgct cgcaacactg
    16621 agtctattat gccaaacaat tggtggatcg cttggcccaa cgattgcctt tgctgatgaa
    16681 attactcatc cacaaacggt aactgtggaa ttggatttgg ctcaccagta tgctgtcgaa
    16741 ggtacattta gcgatggtcg tcccatgtca gaagttaccg ttccgcacta tgcagtttac
    16801 aatggtgtga agcaagatat tttctgtatt gagccagggg tgccgattta caatgagttc
    16861 actcctggtt atgagaaaaa tccattgcct gatatgtcgg agaaagcgaa attagtttcc
    16921 gttttatgga aggaagctgg tacggatgtg gatacacata ttgtggccca gaagatgatt
    16981 tggcaagaag ttaatggcta cacgcttcac tcaattaaac gttctgatgg tagtgcagta
    17041 aatatcgcag ccattgagtc aaaaatcaat caagccattg cggattatca aaagaaacca
    17101 agcttccata actccacagt gaaaaccgtg ttaggtcaat cgactactgt gacagatacg
    17161 aacggcttga atttatcgga gtttgatgaa gttgtcgaga atatcgcaaa tattgattat
    17221 cgtgtgaacg gcaatcaact agtgattact ccaaatgcca attcaaaaga gagtggtgaa
    17281 ttaaccctaa agaagtcagc taatacagga acaccggtag cttataaaat ggctggtcaa
    17341 caaactttga tggcgggggc aattgataag cccaatacct acacaatcaa aattgatgtg
    17401 gaaactgagg gttctttgaa gattaaaaaa gtcgataaag aaacaggaaa tgttgtaccg
    17461 ggaaccgttt tccatttaga ctttggaaaa acattacctg caaaagacgt gaccaccgat
    17521 aaagaaggca ttgcgacatt ggatgagatt cctcatggcg cgaaagtaac cattactgaa
    17581 aaatcagtgc cagctcctta tacgattgac actacaccaa tcaccgcaac cattaaatct
    17641 ggtgagacta tttctgtgac ttcaaaaaat actcgcgaaa aaggtcaaat cattttgaat
    17701 aaaatggggg tggaaacagg aactgatctt tggaacgaca aatactcatt agccggaaat
    17761 acgtttgcca ttcgcaaaga cagtccaacc ggtgaaattg tccaagaaat gaccacggat
    17821 gaaaaaggtc atgcggaaac accaaaagag attgccaatg cgttggaatt gggaacctat
    17881 tatgtgaccg aaaccaaagc tagtaatggc tttgtgaata cctttaaacc agtaaaagtc
    17941 gaattgaact atgtcaatca aaccgtggct cttgttacca gtaacgtaaa agggcaaaac
    18001 caagaaatca ctggtgaaac cactttgaca aaagaagaca aagataccgg taatgaaact
    18061 caaggtaaag ctgagtttaa aggagctgaa tatactctct ttactgcaaa agatggtcaa
    18121 gcggtcaaat ggagtgaggc ttttaaacca gagttagtga agggaacgaa agcttctgat
    18181 gaaacggtga ctttgacttt agatgaaaag aatcaagttg ctgttaaaca cttagcgatt
    18241 aatgagtatt actggcaaga aaccaaagca cctgaaggat ataccttgga tgaaacaaag
    18301 taccctgttt ccatcaaaaa ggtggatgat aacgaaaaaa atgccgtgat tacccgagat
    18361 gttacggcaa aagaacaagt cattcgcttt ggctttgatt tctttaaatt tgctggatca
    18421 gctgctggga ctgccgaaac tggcttcaat gatctgacct ttaaagtatc gccattggaa
    18481 ggaaccagtg agattacaga tgccgaagat gaagcaatta cagcttataa cgagcaacta
    18541 ggttttgatg gctatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa
    18601 gtagaggctc cagaaggatt tcaaaaaatt acgccattag aaatccgttc tacttttaaa
    18661 gaaaataaag cggattacac taagagtgaa tacattttta cgattaccga aaaaggacaa
    18721 aaacaaccaa ttaaaacggt gaccgttcct tacgagaaac tgacgaataa agcattttct
    18781 gttagcttga atcgtttgat gctctatgat ttacctgaag aagaagatag cttaacttct
    18841 cttgggactt ggaaagacgg aggcaaagaa ttgacagacc ttgattctac cgagctagtt
    18901 gataaattga gctataactt gcatgaaatc aaagaagact ggtatgtggt agcgcaagcc
    18961 attgatgtgg aagcgacaaa agctgcccaa gaaaaagatg aaaaagccaa accagtggta
    19021 attgccgaaa caaccgcaac gttggcgaat aaagagaaaa caggaacttg gaaaattttg
    19081 cataaattaa ccgcagaaca agttttggat aaaaccatcg tcttattcaa ctatgtgtat
    19141 gaaaacaaga aaccctatga agcaggtgat gatccagtag cgaaagatgc tagcttgaac
    19201 aatcaagccc aaactgtcaa ttgtacggtg gaacgccatg tttccatcca aacaaaagcc
    19261 cacctagaag atggttcgca aacctttact catggggatg tggtggatat gttcgatgat
    19321 gtgtcgatta cccatgatgt actggatggc tcaaaagaag cttttgaaac aattctgtat
    19381 gcattacttc cggataatac gaccaaagaa atttggaaat ctggcaaaat tgattatgaa
    19441 gtgaatgaca aagaattcac taaaaccgta cttgcggaaa aagtggacac aagtaaatat
    19501 ccagaaggta caagttttac cttcaaagaa attaactatg gcaaagacgg aaacatcaat
    19561 gggaaacaca atgaggatct aaaagaaaaa actcaaactt taacacctaa aaaagttccg
    19621 acaacaccaa gtgcaccaga acaaccggaa ataccaactg ttccaagtga ctctcaagaa
    19681 tctagtccta cagtaaagac attcccacaa acaggggaga aaaattctaa tgttctgctg
    19741 tttattgggt ttaccttgat ctttgcgact gctgggtatt atttctggaa tcgccgaaac
    19801 taaggtgatg tgatgaaaca actaaatagg aaaaaccaac gtgcgcgacc gcctcctaaa
    19861 aaaagtggtc gcgctatttt tgtatccaaa actaaaaatg aaaagaggaa aagaagatgg
    19921 aattgaaatt tgttgtgcca gatatggccg aaacctttgg aaagataagg tatgccggcg
    19981 aaggcgaagt tttaactgaa ggatatgggc ggaatactac agtaattggt cgtagttatc
    20041 atttgtattc cagtaagcaa cgagccgatg atattgaagt ggtggtagct gcggaagccg
    20101 gcgaaaagga ttttgatcaa gatcaaccac taaaagccgt gaatccccat ttggttgcca
    20161 aaggctatga gattgagaat cgtgggttta ccgactacgt gttgtatgtc gatgatttag
    20221 tgaaagcata ggaggaaaag aagatgagat taacagaagg tattgttgta gattcaggac
    20281 taacgtttgg gaaattacga ttttccgcat tacgccgaga agtacggaag caaaatgagg
    20341 atggaacggt tagtaacgag gtaaaagaac ggacttataa tttgaaatcc tctgcgcaag
    20401 gtcgaatgat ccaagttagt atcccagcaa atgtaccctt acgcgagttt gcctatgacg
    20461 cagaggtaga gttggtcaat ccaatcatgg atacggtcgc caattatgtt ttccgagaag
    20521 gaactactgt caattggttc attaaggccg atgatttagt cttgaaacga caaccagatc
    20581 agggaaattc tacaaaccaa aatgaaggaa agaaataggt gaagcgtatg gaaattaatg
    20641 ttgaacatat tttagattgt ctcgatcaat atggaaaagg tgagctaacg gaggaacaac
    20701 tcactaaagc attaacctat gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg
    20761 aagtaggtaa cgatgccgaa gaagattttg atgtactgaa ttggttaggg gaagaagagt
    20821 ccttctttat ggttatcgaa gtaaatgaaa gtctatgtcg agaagccgaa accgtattgg
    20881 aagaaattgg tgtggaaatg ccggatgcca ttgaagtttt tttaaagcaa ctagtggaaa
    20941 cgaaacaact ccctatagca gtaaatgact agcataactg cttgaaattg gatcaatgaa
    21001 atcctatgaa gcttttttcg attggaggtg gaaaccataa aaatgtacaa agggcatcgt
    21061 atacgggcag gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg
    21121 ttcatcggtt ggatgagcgt cttttatttt caagagctta gacaatttga tatttctaag
    21181 ctatcacttt ctactataga aatcgttcgt tctataaaag atttaatcta tttgctagga
    21241 agtctcgttc tttcaggaag cacgatgttg ctttatatac acttttttca ggatcattgg
    21301 cgcagtttgt ggcatcgaca aaagctagcc cggatgattc tagaaaatca ttggtacgaa
    21361 gtaaaacaaa ctcagagtga aggatttttc aaagacctca atagtagtcg aaccaaagag
    21421 acaatcagtt acttccccaa aatctattac cgtatgaagg atggtttact ttctattcgc
    21481 gttcaaattt cactgggaaa atatcaagat cagcttttaa agctggaaaa gaagttagaa
    21541 agtggtctat attgtgaact agtagaaaag gaactcaaag actcctatgt ggagtacact
    21601 ttactgtatg atataattgc caatcgaatt gggatagacg aagtagtggc agaaagtggg
    21661 gctttgcgat tgatgaaaaa tcaagtatgg gcttatgatt ctttacctca tatgttaatc
    21721 gctggcggca caggtggtgg gaagacttac tttttactca ctatcatcga ggctttatta
    21781 aaatctgatg ccgagctttt tgttcttgat cccaaaaatg cggacttagc cgatttaggt
    21841 acggtaatgc ctcatgtcta ttcccaaaag gaggaaattt ctgcttgtgt ggaagatttc
    21901 tatgagcgca tgatgactcg tagcaaggca atgaaagaaa tgcccaacta caaaacagga
    21961 gagaattatg cgtttcttgg acttccacca aactttttaa tctttgatga atacgtggtt
    22021 tatatggaaa tgttaacgac gaaggaaagt gcggtgattt tgaataagct caaacaaatc
    22081 gtgatgttag gtcgtcaatc tggtttcttt ctgattctag cttgtcaaag accggatgcg
    22141 aaatatttag gagacgggat tcgcgatcaa tttaatttct gggtggcttt gggtcgtatg
    22201 agtgaactgg gatattccat gatgtttggc gaggttgata aaaaattctt tatgaagcgt
    22261 atcaaaggtc gaggctatgt agatacggga gggagtgtga ttagtgagtt ttatacgcca
    22321 cttgtaccaa aaggatatga tttcttaagg gaaattagcg acattggtgg attaaacgtt
    22381 aatacggaag gataaaataa tagtatgtag cctaggagtg tcaagaatcc tgggcttttt
    22441 ttacgaggtg atttatgaca tttttagatt tattcgctgg gattggtggc ttttgcttag
    22501 gtatggaaca agcgggtcac cagtgtatcg gcttttgtga aattgatgat tttgctcgcc
    22561 agagttataa ggcaatccat gatacaagta aggaggtgga aatgcatgac atcacaagtg
    22621 tatcagacga gtttattcaa tctctcggac cagtggatat cctttgcggt ggatttccgt
    22681 gccaagcttt ttcaattgcg ggaaagcggc aaggattttc cgatactcga ggtactttat
    22741 tctttgaaat cgctcggttc gccgctattc tccaacctaa gtttttattc cttgagaacg
    22801 tccggggatt actcaatcac gaaggagggg ctacgttcga gacgatcctc cgaacgctgg
    22861 atggattggg gtatgatgtg gaatggcaag tgcttaactc aaaggcctac gttccccaaa
    22921 accgtgagcg gatcttcctt atcggacatt ctcgagacgc atgtaccgaa caagtatttc
    22981 ctatcattgg atcgtctcca acatctgatc aaaacatcag aaacttatta aatatcaacc
    23041 cttccaatcg aggaatgggg ggacaagtct atgggtcaga tgacgtagcg cctaccttga
    23101 ccggtgatga aggaatcaaa atcgcattgc cggtagatga tggaatttct gtagcgggta
    23161 tgttactggg aaattttgaa caaggcaatc gggtttatga gataactgga acagcgccta
    23221 ctttgtcaac gaaacaaggc ggaacgaaga tcatgcttcg gaaaaacgaa acagaatatc
    23281 aagaggtgaa gccagggaac agtgtgaacc tggcttttcc taattccaca agtcgtcgag
    23341 gccgtctggg aaaacaaagt gtgcatacct tattaacagg agaccaacaa gcagtcgtca
    23401 cggatcagta tcaaattcga aagttaacac ctagggagtg ctggcggctt cagggctttc
    23461 cggactgggc ttttgatcga gcttctcaag tcaattcaga tagtcagttg tataagcagg
    23521 ctggaaattc tgtaacagtt ccggtcattt ttgatattgc tagaagatta aaggaggtaa
    23581 aaacagatga tttatgatga gttgattggg gaatctactg ggtgattggc aagattcagt
    23641 cagatccgga attggaagaa gaactgtacc ggctcaattt tgatattcgc aaaaacgggg
    23701 tgaaagtccc tgatgatccg tatgtaattg atgaggaaac ggatgctcga atggagctga
    23761 atcaagtcat cagtgaattg gaacgaattg cggactttgc taaagaacct gatatccgcc
    23821 aatacttgtt tgagataaaa gcagagttgg aaattgaggg tattaccgcg gagtaacggc
    23881 caaataaacc actgataggc actgaccgcg ggggatttgc gaagcagaaa tcctccgtgg
    23941 tgctgtgcct gtttatggac ggcgttagcc ggccatgatg tctcaccccc cgtatctaac
    24001 aggggggtac aaaaacacaa aaaataagca agcaacgata aggaatggct gtcctatcaa
    24061 ggataataag caatttgtgt agatgttaac tgatttgaaa aagttaacac cttaaatttg
    24121 gaggtgagaa cttggcacaa aggaatttag attatcggct attaaaagac cggcgtaatg
    24181 aatatggcgt ttcacaaaat aaattagcta cgacttgtgg acttagtcgc ccgtacttaa
    24241 atcagataga aaatggtggc gtaaccgcat cgacaaaaac catgaggaaa atttttgacc
    24301 aactggaaag tttcaatccc gagttacctt taacgttact gtttgactat gtaaggattc
    24361 gctttcccac aacggatgcg aggaaaatca ttcaagagat tctccatttg aaatttgatt
    24421 atatgctcca cgaagattac gccttttact cctatcaaga acaatatgtt atgggagata
    24481 ttgtggtgat gttgtcacat gaagaagata aaggcgtcct tttagaatta aagggtcgtg
    24541 gttgtcggca gtttgaaact tttttactcg cccaaaagcg tagctggtat gactttttcg
    24601 aagattgtct aaaagccggt ggcgtgatga aacgcttaga cctggcaatc aatgatcgag
    24661 ttggcctgtt agatattcct gatttaacga aaaaatgtca gaaggaagaa tgtatctcct
    24721 tgtttcgtac cttcaaaagc tatcgttccg gggaactgtt gaaacctgat gataaagatg
    24781 gtatgggcaa tactttgtat attggcagtc ttaagagtga ggtctatttc tgtttgtacg
    24841 aaaaagacta tgagcaatat atcaagttag ggattccttt agaccagacc gaaacgaaaa
    24901 atcgttttga aatccgactg aaaaatgatc gagcttatca tgcaattcaa gatttgttaa
    24961 aaggtcgcag tattgaaagt accacatttt ccattattaa ccggtacttg cgatttgcgg
    25021 ataaggtgga aggcaaaagg cgaactaact ggcccttgaa tgaacaatgg gggcgcttca
    25081 ttggacggaa tcagaaggaa attcaattaa cttctgaacc gaagccttat accattgaac
    25141 gaacgttgaa ctggcttgga cgacaagtcg cccctacgtg gaagatggcc aaggagttgg
    25201 atcggttgaa tcagacaacg tacattcagg atatggtaaa taatactcga ctatcagacc
    25261 ggcacaaaaa gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag
    25321 aggaatcgtg atggaaattg tgaaagatat tttattggtg tttggtggca gtatgcttgg
    25381 tgttactgtg atgtgcttga tgcatgcaag tgcagcggcg gaccgagcaa tggaaaagaa
    25441 aggaaagtga tgaaatgaat ttcggtcaaa atttgtatca atggttttta accaatgctc
    25501 aatctttagt cttactagcg attgtggtca ttggtttgtt tttaggattt aaacgcgagt
    25561 tttccaaact gattggcttc ttggtgattg ctttgattgc ggtaggtctg gtctttaatg
    25621 cttctggtgt gaaggatgtc ttactcaatt tgtttaaccg aattgtcggt gcctgagggg
    25681 gaatcacaat ggaacagata cgtgtttata ttgcgaacct tggaaaatac aatgaaggtg
    25741 agttggtggg ggcatggttt accccacccg tcgattttga tgaagtgaaa gaacggattg
    25801 gattaaatga cgaatatgaa gagtacgcaa ttcatgatta tgaactgcct tttgagattg
    25861 atgagtatac accaattgag gaaattaatc ggttgtgtgg tttagcggaa gagcttaaag
    25921 gaacgccaat tggtgaggtc gcttcagaga ttcaacatgc cttcttcaat tctttcgagg
    25981 aaatggtaga gcatgtggat gatatcatct attatcccga ttgtaatgac atgagtgatg
    26041 tagcgtatta cttgattgta gaagctggtg atttaggcga agtaccagaa cacttgaaga
    26101 cgtatattga ttttgatgcg tgtggtcgag acttggagat tggcgggaat tatttggtta
    26161 cgagtcgagg ggtttttgaa ctaatgtaaa tcatttgtta gaattgacca gaaatttcta
    26221 tcgtactttt acttttctat ttgatatact gaaatagaaa atatttgttg ataggggtgt
    26281 tactaatggt tggcaataag tggtacaaat gtgatcttca tattcattca actgcgagtc
    26341 agtgctttca ggataaaaag gttactgcta aggaatgggt agaagaatgt ttaaacaaag
    26401 gactcgattg tgttgcatta actgatcaca atacagggga aaatattgat gaatataaga
    26461 aagtggctat cgaaaatggc ttagattttt ttcctggagt ggaattaacg tgtggagaaa
    26521 atggaactca tttactagtc ctatttgata aaagtgcatg tacaaatgat atctctgatt
    26581 tcttaaccaa gatgggtata aaaagagagg attgggccag cgctgcagct tgctcaatga
    26641 aatcagtttt agaagttatt gattttgctg agcacgaggg cttgctcgtt attcctgcac
    26701 atatagatga gtttaatgga atttcttcag caaaatttgg aaatctagga aagatatttg
    26761 aatcagaaaa tatatatgca gtacagacgg ttcaaaaaga attttttgaa aatcgctcac
    26821 aaatgattcc ctcatctaag cgtgacacta tctatgatag tgtgaatgcc agatatgatg
    26881 gtagagttgg aaaagatact ttggaatctt ggtataaatc agttgttgag gcagaaaaaa
    26941 ataatatgac atttcttagc ttttctgata acccacactc gaaaggaaat tcaaaacatg
    27001 gactttgggg aataggcaca agatattcct atataaaaat gaaggacgaa cctgacttgg
    27061 gatcacttcg cgatgcttta atgctaggcg aaactcgagt tcattctgat ttttctaatt
    27121 tctccattaa tgaaaatgaa gttttattgg aaaaattaag cttttctggg accactctta
    27181 gcacaaaaga agttgtagtc gaattcagcg ataatttaac ttcaattatt ggaggaagag
    27241 gaacaggcaa atcttgtatt acaagattct tggtctatgt ccttggaaag gaagcagaac
    27301 ttgatcaatt ttctgaaata caaagtgatt atcagaactt tgcacaaatt gaacataatg
    27361 gctctggaat atttttgaag gatacaattg tgaaactgaa tattttctat aaggggacaa
    27421 aatatcaaat aataagaaca caggatagac attctattta tgaatttaca caagagaatg
    27481 gtctggttga aaaagaaaca gaacggttga gaatgatttc cgataaggtg agtatatact
    27541 ctcaaaaaca aatttatgaa atttcaaaaa atcaaactag tatactagaa ttactagatg
    27601 gatataactc tgatttgatt tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa
    27661 taagaaaatt aaattgggat attgtttctg tgaaaaaaga aatacaagat aaggcgaagg
    27721 tagagcttga aattgaagat ttaaaaaaac aagtagaaaa gctatctcat aagagttata
    27781 aagatgttta tgatgagtat tcaaaagaga cagatattta tagggaactg aaacgtgatg
    27841 tggaggcact taaggaaatt cccaaaaatc taactgatag tgttgacaaa attgactttg
    27901 gcaatggtat aaaagttaca gacgaaattg atgaacaccg cggagaattg attaagaatt
    27961 tagtgaccaa tcgggctaag attcaaacaa taattaatga aatgtcagat gaaattgata
    28021 gttatagagc agagctgaat aaatcagatt ggaaagttaa ctataatgag gtttcaaaga
    28081 ggtatcaaag aggtatcaaa aacttagtga agagttatct caagatgaat taaaaatgtt
    28141 acaaaacctt gattctttat atcaatcttt atcaaaaact gaagagcgat tggaagaaat
    28201 tattcaacaa gaaataaagc ttaaagatta ccaagttgag gttgactcag caatgtcaag
    28261 aatgaataaa tcatatcaaa atttctattc tgagagaagt agttcctcgc gcgaaatttt
    28321 ttctgatatt acaggaatag aggctactat tcaaccaaat agggatttca ataattatgt
    28381 taaagagctc agagcttgta ttaataagga ggacactttt gacagtgagt ttggatctct
    28441 tattgataag ctgacaaaaa attccattac taatcaagat atatataatg acatacttga
    28501 tataagagtt agtggggaaa gtagtatttt ttcagataaa aaattaatca attctataag
    28561 aaaattgaat gatgcacaag ttcttgaatt acaaaccctt aagccaaatg atttagtagt
    28621 tatccgactt gaactaaatg gaaaaaaagt tagtttatcg aatgctagtg ctggtcaaaa
    28681 gacatctgca attctttcta tgattttggc gtatggaaat tcaccactca tattggatca
    28741 gcctgaagat gatttggata gtcaattaat taataactta atcgtaagaa gtatcattac
    28801 aaaaaaggaa aacagacaaa ttttaattgt aactcataat gcaaatatcc cagttaatgg
    28861 tgattcagaa tggttgatta gcatgggaga ttcaaaagaa atatcggctg atcagtgcgg
    28921 gtcggtagat gaacccaata tcaagccaag aatatgtact gtgatggaag gtggcgagga
    28981 tgcttttaca aatagagcga aacgctatgg ttttaaacaa attgtagaat aacactagag
    29041 ggtgagacat attattatgt ccgccctttt taggaggaaa aatgaaaaaa ataaaaagct
    29101 ataccagtat ctgggcagtt gaaaaagtca tttacgcaat caatgatttt cagttgcctt
    29161 ttcctgtgac ttttaaccaa atggcttggt tcgttttatc tttactgttt gtcatcgtat
    29221 ttgcccatgt gcctccacta tctatgatag aggaggtctt tttaaaatac ttaggcatac
    29281 cggtggctgt cacttggttt atgtcgcaaa agacgtttga tggcaagaaa ccactgggat
    29341 ttttacgatc ctttatcagc tatcacttgc gatttaaggt gacttttgca ggaaagaaag
    29401 tcaaagaaca gaagaaacga tgggatgaac ccagtactct tgtaaggagt gtgaactatg
    29461 tatccgatta agtatattga aaataatctc gtctttaacc aagaaggcga gtgttttgcc
    29521 tactatgaat tagtgcctta caattactcc tttttgtcac ccgaacagaa atttcaagtg
    29581 catgataact ttcgccaact aattgcgcaa aatcgtgaag gcaagatcca tgcgttacaa
    29641 attgccacag aaagtagtat tcgcgccaca caagaacgat caaaaaaaga aattaccggt
    29701 agactcaaag aagtagcgaa acaacgaatt gatatacaga cagatgcatt agtatccatg
    29761 attggcgata gtcagattga ttatcgcttt tttattggct ttaaactaat tgcgacagat
    29821 gaagaagtca atctaaagag tctcaagaaa tctttctttt ctgggcttca agaatttgtc
    29881 tatggagtga atcatcattt gatgggcgat tttgtttcct tatccaatga agaaattcgc
    29941 tgctattcta agcttgaaaa attaatggaa agcaaattgg ctcgaagatt taaagtcagg
    30001 cgagtcacac cgagtgacct gacgtatttg attgaacata tttatggcga aaaagggata
    30061 ccgtttgaag aatacgaatt tcagctacca aagaaaaagc taaaatcaga aacactggtg
    30121 aaacgttatg acttattacg tccaaatcgt tgtttaattg aagaaaaacc aaggtgctta
    30181 cggatggaac atgagaatca tgagtcgtac gtagcgtatt taacaattaa tacaattgtg
    30241 ggggagatgg aatttccttc ctcagaactt ttctattacc agcaacaaca atttacattc
    30301 ccaatcgaca caagtatgaa tgtagagatt gtgacgaata aaaaggcttt agctaccgtt
    30361 cgtaataaga aaaaagaact gaaagattta gacaatcatg cttaccaatc tgacaatgaa
    30421 acgaattcca atgtcttaga tgctttggat tccgtggatg aattggaaac caccttggat
    30481 cagtcaaaag aatctatgta caaactgagt tatgtggttc gagtgagtgc cgaatcagtg
    30541 gatgaactaa agcgacgctg tgatgaagtg ctcgattttt atgatgatac caatgtaaaa
    30601 ctagtccggc cctttggcga tatgatgggg ctacatgaag aattcttacc cttaagtaaa
    30661 cgatatatga acgactatat tcagtatgtt acgtctgatt ttttggcggg gcttggtttt
    30721 ggggctactc agatgctagg agaattagaa gggatttact ttggctacaa tgtggataca
    30781 ggtcgtaatg tttacctgaa accggcattg gcttcacaag gagtcaaggg ttcagtaacc
    30841 aatgcgctgg ccgccgcttt cctgggttct cttggtggag gtaaatcctt tagtaacaat
    30901 ctcttagtct actatgctgt tttgttcggt gg
//
LOCUS       DACERL010000029        30532 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.030, whole genome shotgun sequence.
ACCESSION   DACERL010000029 DACERL010000000
VERSION     DACERL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30532
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.030"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IXF54_001351"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IXF54_001351"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1404625.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="IXF54_001352"
     CDS             complement(2423..3118)
                     /locus_tag="IXF54_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1404626.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IXF54_001353"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IXF54_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1404627.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="IXF54_001354"
     CDS             complement(4317..4508)
                     /locus_tag="IXF54_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1404628.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="IXF54_001355"
     CDS             complement(4529..5419)
                     /locus_tag="IXF54_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1404629.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="IXF54_001356"
     CDS             complement(5406..6173)
                     /locus_tag="IXF54_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1404630.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IXF54_001357"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IXF54_001357"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1404631.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IXF54_001358"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IXF54_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1404632.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IXF54_001359"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IXF54_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1404633.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10789..11127
                     /locus_tag="IXF54_001360"
     CDS             10789..11127
                     /locus_tag="IXF54_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404634.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11295..12053)
                     /locus_tag="IXF54_001361"
     CDS             complement(11295..12053)
                     /locus_tag="IXF54_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1404635.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="IXF54_001362"
     CDS             complement(12071..12889)
                     /locus_tag="IXF54_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1404636.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IXF54_001363"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IXF54_001363"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1404637.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IXF54_001364"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IXF54_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1404638.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14017..15351
                     /gene="dnaA"
                     /locus_tag="IXF54_001365"
     CDS             14017..15351
                     /gene="dnaA"
                     /locus_tag="IXF54_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1404639.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15549..16679
                     /gene="dnaN"
                     /locus_tag="IXF54_001366"
     CDS             15549..16679
                     /gene="dnaN"
                     /locus_tag="IXF54_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1404640.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16908..17150
                     /gene="yaaA"
                     /locus_tag="IXF54_001367"
     CDS             16908..17150
                     /gene="yaaA"
                     /locus_tag="IXF54_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1404641.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            17137..18261
                     /gene="recF"
                     /locus_tag="IXF54_001368"
     CDS             17137..18261
                     /gene="recF"
                     /locus_tag="IXF54_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1404642.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            18258..20204
                     /gene="gyrB"
                     /locus_tag="IXF54_001369"
     CDS             18258..20204
                     /gene="gyrB"
                     /locus_tag="IXF54_001369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1404643.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            20224..22695
                     /gene="gyrA"
                     /locus_tag="IXF54_001370"
     CDS             20224..22695
                     /gene="gyrA"
                     /locus_tag="IXF54_001370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1404644.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22931..23230
                     /locus_tag="IXF54_001371"
     CDS             22931..23230
                     /locus_tag="IXF54_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1404645.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            23280..23792
                     /gene="ssb"
                     /locus_tag="IXF54_001372"
     CDS             23280..23792
                     /gene="ssb"
                     /locus_tag="IXF54_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1404646.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23818..24054
                     /gene="rpsR"
                     /locus_tag="IXF54_001373"
     CDS             23818..24054
                     /gene="rpsR"
                     /locus_tag="IXF54_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1404647.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            24197..26170
                     /locus_tag="IXF54_001374"
     CDS             24197..26170
                     /locus_tag="IXF54_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1404648.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            26176..26628
                     /gene="rplI"
                     /locus_tag="IXF54_001375"
     CDS             26176..26628
                     /gene="rplI"
                     /locus_tag="IXF54_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1404649.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26902..28269
                     /gene="dnaB"
                     /locus_tag="IXF54_001376"
     CDS             26902..28269
                     /gene="dnaB"
                     /locus_tag="IXF54_001376"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1404650.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            29049..30341
                     /locus_tag="IXF54_001377"
     CDS             29049..30341
                     /locus_tag="IXF54_001377"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1404651.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9220 a         6027 c         6033 g         9252 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaaaggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc
    13801 aagaggaaat ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat
    13921 taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac
    13981 tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg
    14041 aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc
    14101 gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag
    14161 attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg
    14221 ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa
    14281 gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg
    14341 aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca
    14401 gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga
    14461 ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt
    14521 cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat
    14581 tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg
    14641 ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat
    14701 actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca
    14761 aacgagattc cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt
    14821 gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct
    14881 gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac
    14941 attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa
    15001 gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat
    15061 cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg
    15121 aaagatctca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg
    15181 tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc
    15241 aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt
    15301 gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat
    15361 tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact
    15421 ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac
    15481 atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga
    15541 gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc
    15601 aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga
    15661 cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt
    15721 ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag
    15781 cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt
    15841 tagaaaataa acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag
    15901 atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg
    15961 tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc
    16021 gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta
    16081 ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg
    16141 atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag
    16201 aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt
    16261 tctattcccg tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt
    16321 ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt
    16381 tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg
    16441 ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa
    16501 gcagtggcga tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg
    16561 catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg
    16621 aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac
    16681 aatgattttc aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa
    16741 acagaaggcc ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt
    16801 ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa
    16861 aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag
    16921 tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg
    16981 gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac
    17041 cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag
    17101 gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta
    17161 aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt
    17221 ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg
    17281 acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg
    17341 atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct
    17401 aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg
    17461 ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg
    17521 cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat
    17581 gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat
    17641 gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt
    17701 ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat
    17761 tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca
    17821 gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg
    17881 aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac
    17941 gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa
    18001 agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg
    18061 gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc
    18121 catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac
    18181 catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta
    18241 aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg
    18301 atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt
    18361 atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt
    18421 cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca
    18481 acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg
    18541 gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg
    18601 gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgta aacgcgcttt
    18661 ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc
    18721 gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg
    18781 ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat
    18841 tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag
    18901 acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg atcaaaagtt
    18961 atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag
    19021 gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca
    19081 atatcatgag ttttgccaac aatatccata cgtatgaagg tggaacacat gaatcaggat
    19141 tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag
    19201 aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta
    19261 tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag
    19321 tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc
    19381 caagtgtggg ccggcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag
    19441 cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag
    19501 gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag
    19561 gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc
    19621 ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg
    19681 aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat
    19741 cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata
    19801 tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat
    19861 acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg
    19921 tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct
    19981 caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat
    20041 gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta
    20101 ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat
    20161 tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa
    20221 agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa
    20281 gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga
    20341 gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca
    20401 ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat
    20461 cctcatggag acattgcgat ctatgagtca atggtacgga tggcacagcc attcagctac
    20521 cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc
    20581 gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc
    20641 aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta
    20701 ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg
    20761 gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg
    20821 gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca
    20881 acaggtggtt tggttatggg gaaatcagga atccgaagag cgtatgaaac aggtaagggt
    20941 tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc
    21001 atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa
    21061 ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa
    21121 ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catccgttat cttgaataac
    21181 ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa
    21241 ggtgtgccga aagttttgag cttgaaacaa atccttgaaa actatatcga acatcaacgc
    21301 gaagtcatca cacgacggac ggtctttgaa aaagaaaaag cagaagcaag agcacacatc
    21361 ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg
    21421 tctaaatcag atgacgaggc aaaagcaaca atgatcgaac ggttcgattt atccgatcgc
    21481 cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa
    21541 atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca
    21601 agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt
    21661 ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat
    21721 ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg
    21781 gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc
    21841 cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc
    21901 tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgt
    21961 acagccaaag gaatcccaat catcaattta ctaggtatcg attcgagtga aagcatccag
    22021 gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt
    22081 caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg
    22141 atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa
    22201 tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc
    22261 cgcgatatgg gacgtacagc ttccggggtc agagggatac gcctgcgtga ggaagattat
    22321 gtcgttgggg cagccttaat gaaggaaaat caagaagtat tagtgatcac agaaaaaggt
    22381 tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc
    22441 aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga
    22501 gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact
    22561 gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg
    22621 aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa
    22681 ggatcagaag aataaccatc aagactaagt gtgtaagcta gcaagaataa ccgattaaaa
    22741 aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac
    22801 tgttcattct gtattgcttt tgaccaaaat agagtgtata attagagctt gtgagtagtc
    22861 taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag
    22921 gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga
    22981 tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc
    23041 agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta
    23101 ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa
    23161 tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga
    23221 agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt
    23281 tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag
    23341 caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa
    23401 gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt
    23461 tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt
    23521 cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc
    23581 aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt
    23641 ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat
    23701 ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga
    23761 tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg
    23821 gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat
    23881 attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt
    23941 aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt
    24001 gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc
    24061 ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa
    24121 taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat
    24181 atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct
    24241 tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag
    24301 cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg
    24361 ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa
    24421 tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca
    24481 aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga
    24541 ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct
    24601 agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga
    24661 tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat
    24721 tttatctatt gataactatg cagatgccat tgataaatta gatgacaaag agatttctta
    24781 tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt
    24841 caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat
    24901 gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc
    24961 gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg
    25021 agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa
    25081 agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa
    25141 acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac
    25201 aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt
    25261 tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa
    25321 cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga
    25381 aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt
    25441 gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga
    25501 taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct
    25561 gacttatatt gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata
    25621 ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat
    25681 ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag
    25741 ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt
    25801 agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat
    25861 tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc
    25921 agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga
    25981 cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga
    26041 atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt
    26101 tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga
    26161 taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa
    26221 ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg
    26281 gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa
    26341 aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa
    26401 gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc
    26461 ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga
    26521 aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt
    26581 catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata
    26641 gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag
    26701 aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc
    26761 ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt
    26821 ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat
    26881 tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat
    26941 attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat
    27001 gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag
    27061 acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg
    27121 ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca
    27181 ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca
    27241 ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga
    27301 gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa
    27361 agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc
    27421 gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg
    27481 gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg
    27541 gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt
    27601 tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt
    27661 gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg
    27721 tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca
    27781 ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc
    27841 aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac
    27901 cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa
    27961 gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga
    28021 ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc
    28081 ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag
    28141 ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga
    28201 acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa
    28261 gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc
    28321 aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt
    28381 gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt
    28441 ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata
    28501 ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact
    28561 cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt
    28621 gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg
    28681 catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt
    28741 tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt
    28801 agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt
    28861 ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta
    28921 aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt
    28981 tatttgaaat atgtatctat tattgataca atggttcagg attaaataat atagaacgag
    29041 gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa
    29101 aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca
    29161 atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg
    29221 ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat
    29281 cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta
    29341 tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag
    29401 atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg
    29461 ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag
    29521 aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag
    29581 aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac
    29641 aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt
    29701 tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta
    29761 cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa
    29821 tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc
    29881 caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta
    29941 cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta
    30001 aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg
    30061 aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag
    30121 caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag
    30181 aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac
    30241 gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa
    30301 cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga
    30361 atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga
    30421 agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg aacactgttt
    30481 attcagaata agggactgtg acaaaaaccg tgtcacagtc cctttttccg aa
//
LOCUS       DACERL010000030        28598 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.031, whole genome shotgun sequence.
ACCESSION   DACERL010000030 DACERL010000000
VERSION     DACERL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28598
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.031"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            236..1876
                     /locus_tag="IXF54_001378"
     CDS             236..1876
                     /locus_tag="IXF54_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1404652.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="IXF54_001379"
     CDS             2030..3352
                     /locus_tag="IXF54_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1404653.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="IXF54_001380"
     CDS             complement(3414..3845)
                     /locus_tag="IXF54_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1404654.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="IXF54_001381"
     CDS             complement(3909..4745)
                     /locus_tag="IXF54_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1404655.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="IXF54_001382"
     CDS             complement(4935..5237)
                     /locus_tag="IXF54_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1404656.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IXF54_001383"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IXF54_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1404657.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="IXF54_001384"
     CDS             complement(5691..5957)
                     /locus_tag="IXF54_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1404658.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="IXF54_001385"
     CDS             complement(6211..6936)
                     /locus_tag="IXF54_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1404659.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="IXF54_001386"
     tRNA            complement(7302..7372)
                     /locus_tag="IXF54_001386"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="IXF54_001387"
     CDS             7489..8241
                     /locus_tag="IXF54_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1404660.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="IXF54_001388"
     CDS             8257..8736
                     /locus_tag="IXF54_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1404661.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="IXF54_001389"
     CDS             complement(8987..10567)
                     /locus_tag="IXF54_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1404662.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSVLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="IXF54_001390"
     CDS             complement(10560..11303)
                     /locus_tag="IXF54_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404663.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="IXF54_001391"
     CDS             complement(11479..12255)
                     /locus_tag="IXF54_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1404664.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="IXF54_001392"
     CDS             complement(12503..13339)
                     /locus_tag="IXF54_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1404665.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="IXF54_001393"
     CDS             complement(13350..14279)
                     /locus_tag="IXF54_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1404666.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="IXF54_001394"
     CDS             14471..14824
                     /locus_tag="IXF54_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1404667.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="IXF54_001395"
     CDS             complement(14898..15554)
                     /locus_tag="IXF54_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1404668.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="IXF54_001396"
     CDS             15780..17714
                     /locus_tag="IXF54_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1404669.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="IXF54_001397"
     CDS             complement(17800..19023)
                     /locus_tag="IXF54_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1404670.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..20915)
                     /locus_tag="IXF54_001398"
     CDS             complement(19017..20915)
                     /locus_tag="IXF54_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1404671.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20909..22027)
                     /locus_tag="IXF54_001399"
     CDS             complement(20909..22027)
                     /locus_tag="IXF54_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1404672.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22110..22943)
                     /locus_tag="IXF54_001400"
     CDS             complement(22110..22943)
                     /locus_tag="IXF54_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404673.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22936..23853)
                     /locus_tag="IXF54_001401"
     CDS             complement(22936..23853)
                     /locus_tag="IXF54_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404674.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23876..24055)
                     /locus_tag="IXF54_001402"
     CDS             complement(23876..24055)
                     /locus_tag="IXF54_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404675.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24080..25204)
                     /locus_tag="IXF54_001403"
     CDS             complement(24080..25204)
                     /locus_tag="IXF54_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1404676.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25387..26295
                     /locus_tag="IXF54_001404"
     CDS             25387..26295
                     /locus_tag="IXF54_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1404677.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26436..28049
                     /locus_tag="IXF54_001405"
     CDS             26436..28049
                     /locus_tag="IXF54_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1404678.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8890 a         5620 c         4868 g         9220 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagcact gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    20521 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    20581 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    20641 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    20701 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    20761 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    20821 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    20881 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    20941 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21001 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21061 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21121 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21181 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21241 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21301 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21361 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    21421 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    21481 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    21541 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    21601 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    21661 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    21721 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    21781 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    21841 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    21901 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    21961 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22021 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22081 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22141 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22201 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22261 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22321 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22381 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    22441 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    22501 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    22561 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    22621 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    22681 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    22741 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    22801 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    22861 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    22921 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    22981 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23041 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23101 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23161 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23221 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23281 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23341 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    23401 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    23461 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    23521 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    23581 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    23641 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    23701 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    23761 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    23821 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    23881 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    23941 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24001 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24061 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24121 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24181 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24241 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24301 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24361 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    24421 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    24481 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    24541 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    24601 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    24661 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    24721 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    24781 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    24841 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    24901 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    24961 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25021 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25081 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25141 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25201 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    25261 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25321 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25381 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    25441 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    25501 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    25561 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    25621 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    25681 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    25741 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    25801 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    25861 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    25921 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    25981 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26041 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26101 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26161 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26221 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26281 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26341 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    26401 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    26461 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    26521 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    26581 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    26641 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    26701 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    26761 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    26821 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    26881 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    26941 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27001 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27061 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27121 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27181 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27241 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27301 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27361 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    27421 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    27481 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    27541 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    27601 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    27661 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    27721 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    27781 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    27841 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    27901 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    27961 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    28021 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28081 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28141 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28201 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28261 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28321 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28381 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    28441 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    28501 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    28561 tgtattaaat aatttaattt ttttgttcct atttattt
//
LOCUS       DACERL010000031        28073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.032, whole genome shotgun sequence.
ACCESSION   DACERL010000031 DACERL010000000
VERSION     DACERL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28073
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.032"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(80..661)
                     /locus_tag="IXF54_001406"
     CDS             complement(80..661)
                     /locus_tag="IXF54_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1404679.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(844..1470)
                     /locus_tag="IXF54_001407"
     CDS             complement(844..1470)
                     /locus_tag="IXF54_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1404680.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1492..2823)
                     /locus_tag="IXF54_001408"
     CDS             complement(1492..2823)
                     /locus_tag="IXF54_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1404681.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2978..3379)
                     /locus_tag="IXF54_001409"
     CDS             complement(2978..3379)
                     /locus_tag="IXF54_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1404682.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3465..3671)
                     /locus_tag="IXF54_001410"
     CDS             complement(3465..3671)
                     /locus_tag="IXF54_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404683.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3766..5268)
                     /locus_tag="IXF54_001411"
     CDS             complement(3766..5268)
                     /locus_tag="IXF54_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1404684.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5286..5741)
                     /gene="argR"
                     /locus_tag="IXF54_001412"
     CDS             complement(5286..5741)
                     /gene="argR"
                     /locus_tag="IXF54_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1404685.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5839..6456)
                     /locus_tag="IXF54_001413"
     CDS             complement(5839..6456)
                     /locus_tag="IXF54_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404686.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6677..8050
                     /locus_tag="IXF54_001414"
     CDS             6677..8050
                     /locus_tag="IXF54_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1404687.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8125..9237
                     /locus_tag="IXF54_001415"
     CDS             8125..9237
                     /locus_tag="IXF54_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1404688.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9309..10946)
                     /locus_tag="IXF54_001416"
     CDS             complement(9309..10946)
                     /locus_tag="IXF54_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1404689.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11114..11722)
                     /locus_tag="IXF54_001417"
     CDS             complement(11114..11722)
                     /locus_tag="IXF54_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1404690.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12106..13257)
                     /gene="queG"
                     /locus_tag="IXF54_001418"
     CDS             complement(12106..13257)
                     /gene="queG"
                     /locus_tag="IXF54_001418"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1404691.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13276..14028)
                     /locus_tag="IXF54_001419"
     CDS             complement(13276..14028)
                     /locus_tag="IXF54_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404692.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14169..15119)
                     /locus_tag="IXF54_001420"
     CDS             complement(14169..15119)
                     /locus_tag="IXF54_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404693.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15120..15959)
                     /locus_tag="IXF54_001421"
     CDS             complement(15120..15959)
                     /locus_tag="IXF54_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1404694.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15956..16702)
                     /locus_tag="IXF54_001422"
     CDS             complement(15956..16702)
                     /locus_tag="IXF54_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404695.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16940..18310
                     /locus_tag="IXF54_001423"
     CDS             16940..18310
                     /locus_tag="IXF54_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1404696.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18365..19261)
                     /locus_tag="IXF54_001424"
     CDS             complement(18365..19261)
                     /locus_tag="IXF54_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1404697.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19542..20219)
                     /locus_tag="IXF54_001425"
     CDS             complement(19542..20219)
                     /locus_tag="IXF54_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1404698.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20310..21215)
                     /locus_tag="IXF54_001426"
     CDS             complement(20310..21215)
                     /locus_tag="IXF54_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1404699.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21390..22844
                     /locus_tag="IXF54_001427"
     CDS             21390..22844
                     /locus_tag="IXF54_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1404700.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22860..23891
                     /locus_tag="IXF54_001428"
     CDS             22860..23891
                     /locus_tag="IXF54_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1404701.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23898..25085
                     /locus_tag="IXF54_001429"
     CDS             23898..25085
                     /locus_tag="IXF54_001429"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1404702.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25241..26623
                     /locus_tag="IXF54_001430"
     CDS             25241..26623
                     /locus_tag="IXF54_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1404703.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26750..26980
                     /locus_tag="IXF54_001431"
     CDS             26750..26980
                     /locus_tag="IXF54_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404704.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27180..27848)
                     /locus_tag="IXF54_001432"
     CDS             complement(27180..27848)
                     /locus_tag="IXF54_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1404705.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7817 a         5745 c         5154 g         9357 t
ORIGIN      
        1 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
       61 ctgtcacaac ctctaattat taaagaacct tttgcgaagt taacaatctt tctttgatca
      121 attgaaaatt ctgcctaagt agtgatagat cctgtgtgac tttcaacaat aagatacttc
      181 cctggatttc agcaatcaac gctttggctt gacggtctgc ttcttcatcg gacagttttt
      241 cttcagaaag tgccaggata ttttgtttcg tatacttgac ccattgattg actaattcta
      301 gtagcaacag acggtattct tcatccactg tactcaactc aataatcaaa ttacctacag
      361 gacagccatg ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc aacatttctt
      421 ctaatgcttc ttctggctgt tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc
      481 atacgtccat atgcgcatgg atcaccgcta gacaaacttc tttttttgaa gaaaagtaat
      541 aataaaattg accttttcct gtatcagaag ctgctagaat atctcggatt gacgtttcat
      601 gatagccttt tttataaata agatctttgg tcgcttccaa gatcttttcc tttgttttca
      661 atgatattct cctttttcat ttctcatagt cctatattat acagtaaatt catcattttt
      721 tcttcttaat cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa
      781 ctgttctcat atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct
      841 atcttatgat agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc
      901 aaagttcgtc aacgctgcat ccattgcttc ttggccagga gcaccaattg catctgtcgc
      961 taccacaaca ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt
     1021 tccgttcatt ccaccaagaa ttactgtgtc gattccattt tttctcaatt ggagcgccaa
     1081 atcattgctt tctggtccaa agattttatg aggagagctg atgatcgtat tttcatccaa
     1141 ataaggtttc aattcttcga taaaatcggc tcctgaacct tcttccactg attcatattg
     1201 actttttcta gcaaacatct gctgctcaag catcatatgt tctcctgcac cgccaaattg
     1261 ccatttatgg tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc
     1321 ttttgcttgt tttaccattt caatcatatt ttcgatcgta ttgtattttt ctaagatatc
     1381 ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag
     1441 aactactgct gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa
     1501 tacatcattt agagcttcac tcattgtcgg atgagaatag atttgatctc tcaataacgt
     1561 ataaggaagt tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc
     1621 cgcatagata cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac
     1681 acctttagga tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa
     1741 tttgtaattg acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt
     1801 cggagtgata aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc
     1861 gcccttcaac tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa
     1921 tccgcctttg acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc
     1981 cacagcgatt gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc
     2041 tgtacttggt tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg
     2101 gccatcttct gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga
     2161 tacacctaga tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat
     2221 atcttcatct tcacgtggaa gaaattcaga atgggcatca agaacgacaa ctttagatcc
     2281 gtattcattg aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa
     2341 tttttccggt aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt
     2401 attcaatcct ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc
     2461 gccttcaaca atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa
     2521 acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt
     2581 ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc
     2641 tctttctcca ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc
     2701 gccatacata cgtgttgact tttcaacgac taaaaccgat tcaccttttg aagctaacgc
     2761 cttcgctaaa gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat
     2821 catttttccc tcgcttcttt tttacctacc gattggtaca aaaatatgat aaccgataat
     2881 tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa aagcggtcct
     2941 ttcacgtttt tagtctggtt cgatttcctt tattcttcta aacaatcgtt acttctacat
     3001 cgatatttcc tctagttgct ttggagtaag ggcagaattc atgcgcatct tctgcaattt
     3061 tttgggcttc ttcttcgctg atgccatcga tatgcacttc taatactaca ccgatctgat
     3121 agccgccatc atctaaatta taaagagaga cacgtgcttt tactgtggac tctgctgtca
     3181 ttcctgcttc tttgatcata tgttgtaccg ccccattgaa acaagaagca taacctgctg
     3241 caaataattg ttctggattt gttccttctg catggattcc tggaggagtg attttcatat
     3301 gcatcgagcc atttggtgcc tcgacttctc cattgcgccc gccatggttg atcattgctg
     3361 tttcgtatgc ttttttcatt gtactgctcc tttcgattga ccatttgcta cttggctata
     3421 attatagtgt atgacaagta aggcgctttc tacaaaaatt ctgcttattc gaataccaga
     3481 atgccgtctt ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc
     3541 atatttgttc caaaataaat agcaatcttt tctacataat cttttatatg attgatttta
     3601 agatacatat ataccagaaa gttgacccca taaaccaata ctgttaaaaa aataaccata
     3661 ttcgttacca ttttttcctc cttttgctgg tctccctacc agcactcgat ttaaccatac
     3721 cacttttttt gccgtccaag caactagcct ttcaaaaaga ctggtttatt ctgctgttaa
     3781 tgattcaaga tcagaaaaat gtccgaataa ttttgctaat tttactaacg ccaacttgtt
     3841 ctttccgaat tcatcatttt ctatctgtaa tactagtaac ggctcatgta agcttaaacg
     3901 gatcaaaaac cagccgtttc cgtatggctc ttctagatca tatcgaatac cttcttcatt
     3961 ttccgggtcg aagttcatcc ctttttcttc ttccaacagt cgcttcattt cttctaatat
     4021 cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt aatcggattt cttgtacttc
     4081 tacgggctgt ttcaactctt tgatttgttc atctaatgtg ataccttttc tttttaattc
     4141 tggcaaaagc atcaggatct ttgcgatgac atatgcacca tcatccagaa aatagttttc
     4201 cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt gtatctttcc caagacggtt
     4261 aagttctaat gctttattga ttacatttcg atagccggaa atataacgaa cttggatgcc
     4321 tcctttttct tcaatgaatc tttgtagatg agaagaagta ggggaattcg tcacgattct
     4381 tgttcctgga tgctctttta atacgattgt acttaatact gcgatcaaat tatttcgatt
     4441 gatcactgtt ccagaagcac tgacaactgc agaacgatcc acatctgtat cgaaaatgat
     4501 cccaagatct gcttcatttg ctaaaacagc ctgcttgata ctttccatcg cttctttatt
     4561 atcaggattt gggatatgat taggaaatcg gccatctggt tccaaaaatt gggaacctgt
     4621 tgaatccgca cctaagctat tcaatacttt ttcagcaaag aatccaccag caccatttcc
     4681 ggcatcaaca atgattttcc aaccatttag tggtttgctt tctagtgttc caatcccctt
     4741 tttaattttt gtgactaaat cttgcgcata aggtgtcaaa aggtctgctt tttctacgct
     4801 tccagtttgt tcgcttttta ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt
     4861 ttctgctcct ccttctgagg taaagaattt tattccatta taataatagg gaagatggct
     4921 agcagtcagc ataaccgcag catcgcacga gaactgttca aattgagtgg acataaacat
     4981 agcaggcgta gtagctaatt cgaaatcgat gacatgtatc ccttgttctt tcagactttg
     5041 gatcaacgcc tctttcagtg ctggtccgct tatcctactg tcatgaccaa tcccaatcgt
     5101 ccatttttta tttggatcgg ttgatttctt ctttttcttt atccagtttg ctagtcccca
     5161 gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc
     5221 gattcctcga atatccgatc cattttgcag cgctttcata tctttcatat aaaagtcctc
     5281 ctcatttatt ccctcatttt ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag
     5341 cggcaaataa taatgaatgt atctacaccg gccatcgttc cgacaacttc agggtaagcc
     5401 acttcatcta gccaattcgt taccacatcg gcaccatttc gttcagtcaa tacgataacc
     5461 atgaactgaa cagactggat acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct
     5521 tcatttaaag aaacagatgg ctggttaaat aatgtatagc gtactatttt gttttcattt
     5581 tgacttttta ctaacttcat ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg
     5641 gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta
     5701 atgatctggc ggatcagtgc ttgtcgctgt ttctttttca tattagacac ctctcaatgt
     5761 tgattatagc aaaagataag taatatttct tcatattttg gtatttttta cttttatttt
     5821 ttgttttcat tcaggttgct aacctttttc tattttaaca cgcaaaatcg tcttcatttt
     5881 ttcaggtgct gattttcttg gatcgctgat gtaaatctct ttgtgttctt tagaagctct
     5941 ttcatagcct tcttttgcga tgaatgcttc taatttctca aacgttgctg gttcttcggc
     6001 atagggcccg acgtgtaaga tttgggcaca aagaccttct tccatctttt tgaatactac
     6061 ttgttctttc aagtcttccg gaagtttgct agaacgctga atggcttctt gtgctatcgc
     6121 ttctgtaaca aagtcaggct gtcggatcat caattgataa gaaaaatgtt cttttttcat
     6181 cactggttca tgcaaatact tttcttgcag tccccattgt ccttccagtg gatagacagt
     6241 atacatctga taatcaggaa tcagccaatt ttttttaggc gacatgcgta ttgcatagct
     6301 gactgcatat agacaggcta ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc
     6361 tctgatgcta aagaatgatt ttttaggaac ctctagtatt tgcgggcttt ttttcatact
     6421 atagctcttg tctttacgcc attcaatttt ttccattttt cttcctccgc gaaattccat
     6481 ttagttattt attatcatac ctctttttga gtgacttggc taatcgtgtt gacactattt
     6541 ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac agataacacg tttcggcgtg
     6601 tcgagagaga ggcggtttct atttttataa tagaaatcac tctctttttt tacgaaattt
     6661 tttagaagtg atgtaaatga caaaacaaaa acagctgctt ttagtacctg cagtaattgc
     6721 aacagggatg atgtcttttg cgggcgtatt gatcgagacg gccatgaatg taactttccc
     6781 aactcttatc agacagtttg atttaacgac tgcacaagtc caatgggtaa caacgattta
     6841 tcttttgatg atttcgatca ttgttccgct gtctacttat ttgaatcgca attttagtct
     6901 caaaaaactc tttttagctt caaatctgct tttcttggca ggtataatga tcgatttctt
     6961 ctctcctacc ttcatggtcc tattgtttgg tcgattgctc caaggtgcag cgacaggtat
     7021 cgctcttccg ttgatgttcc atattatctt gacatttgcc cccctcaatc gacgaggcgc
     7081 gatgatgggt gtcggtactt tgacaacggc cattgctcca gcaattggcc ccacttatgg
     7141 aggcattatg acttcacatt ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt
     7201 gtttctttct ttgattatcg gctggtttgc tattcccgag atggcagtac aaaaaagtgg
     7261 ttatttggat tggataagcg ttttaggaat ttgtcttttg tttagtggaa gtctaacatt
     7321 tttgagtacg ttgggaacag tttccagctg gattgcttta atcatcggcg gtattggctg
     7381 gttccttttt tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac tgtctattct
     7441 ttcaaataaa acattccgct tgtttttgat tggcttctta gtttatcagt ttttattact
     7501 tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt caaggaagta gtccttttgt
     7561 cgctggatta gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc ctttttccgg
     7621 gaaaatgctg gatcaatatg gtccgaagaa gccaattttt gctgggctga ttctttcttt
     7681 gattggttgg ctagcattat tcctactcat aggacacgct tcagtgggtg tattgattgc
     7741 ttgtcatgtg ttttatatga tcggtatcgg attatcttac agcaatatga tgaccaccgg
     7801 aatgaatgca ctgaatgaag aactacaagg agacgggaac gcaatgttca ataccttgca
     7861 gcagttttct ggcgctgtgg ctacttcatt agtagcggtc attatcaatt acgtccagca
     7921 tcatacttcc cataactatg aagtgtctac tacattagga tcaaaagcag cattaggcgt
     7981 cttgttgctg ttgcttctcg taagctttgc tcgattcgcc tattatctgt tctttgctaa
     8041 aaaagcgtag attccatata cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg
     8101 ttgtttgtaa aaggaggctt ttttatggaa aaaattgcca ttatcggtgg cgggatcatt
     8161 ggtatgaccc tagccagtta cttagataca tcaaaattca atgttgcttt atttgatgaa
     8221 gggacaggac aggcaactaa agctagcgct gggattatct ccccttggtt gtcgaaacgc
     8281 agaaacaaac aatggtatcg tctggctaaa gacggcgctg cttttttccc taaacttata
     8341 aaagatttcc aacttgatca aacgatttac gcacaatcag ggacactttt gcttcgacct
     8401 gaaaaagatt tagctgaatt ggctgttctt gccgagaaac gaaaagaaga agcgccagaa
     8461 attggaaaca tcgaatggct ttcacctgaa cagacatctc aagcacttcc gttattgaaa
     8521 ccagcagttt cactgcgtat cagtggtggt ggtcgtctgg atggaaaagc atatttagaa
     8581 catcttggtc agcgtttagc acaaaaacaa atggagcaga tccaggcaaa agtcactttg
     8641 cataagaaga gcgattcttg gctcatcaag ggaccgtttg gagaatacac agctgatcgt
     8701 gtaattttga cacctggccc ggcacttaag cctttattac aagaattagg ctatgctgca
     8761 gatatacgtc cgcaaaaagg gcagctctta gcatttcaga catcttataa gatgagtcaa
     8821 gactggcctg ttgctatgct tgacggagaa gctgatttca ttcctttcat ggatggaaag
     8881 atccttttag gtgctaccca tgaaaacgat caaggctggg atttggctcc tactagcgaa
     8941 gcttataagg gattgactaa gagtacagaa cagttcttga ctgatccaga tcagataaaa
     9001 aactggccat acgagttccg tgtaggtacc cgtgcttata cgtcagattt ttccccattt
     9061 ttcggcgtac tgccagatga tccaagctta ctggctgcta gcggtctagg ctcttctggc
     9121 ttgaccacag gtccttatat cggctatcta ttagccaact tcttaaatac tggtcattgg
     9181 cctacggaag attatcaaaa ggacatctcc acctatatta cgaaaaatcc atcatagaaa
     9241 aacagctttt tcaagtaagg atcccgagcg atattgcgca tggattactt cttgaaaaag
     9301 ctgttatttt agttcaattt ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc
     9361 aagagatctt ttcctatctg agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc
     9421 accagatttt tataggacgt atcctcaaaa gaaacaggaa aatctggtat aggtaaatag
     9481 tggatgtctt caaatggacc aaacgacttg aattctgccg tttgttgcac gattctttca
     9541 acgattgaca atgtcgtttc aggtttgatt tttttatcat cgaaatatcc agtattcagg
     9601 acatagcagg ctgtttgctg tttcaaaaac aactctcgaa aagcttgata gtcttcccta
     9661 agaggataga cacggaaagg attagcaaat ggttcaatga ctagctcatc tagctgtctt
     9721 tttgtagtga tattttccgc gtctgatcgt tttgttgcta atgttgcacc aaaagacgct
     9781 gctaaaactg ggtcattgat cttgataatt ggcggaagcg aatcatcttt catgatccaa
     9841 aaaacagcat cgatcttttc gctaagatga tcgactcggt cgggtgtcgc ataacgtgat
     9901 ttgactgtcc gcccatttcc gtttcgtata tctcctgtta ctaggacttt ttctccttta
     9961 cttgtacatg tcacgcccac attctgacaa gtaaggaaag aagaaatggc cggatcatca
    10021 gacggatagt cttgtgtttt atcaaagtaa gaaggttcca aggcagtagc aaaaccattt
    10081 tttcgagaaa taacgaaagc atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt
    10141 tttgctaagg taatcgttga ttttccagaa ccggacaacc caaatacagc catcgtatag
    10201 gtctggtcat tactagcata ttgtttcatc ccaccgtggc aggcaataaa gccattgcga
    10261 tttcccaatg cccaagcaag cgtcaaagtc gcttttttta attctccaaa gtaacgtaat
    10321 cctagcacag ccgctgtatt atggagagca tcaaacaaaa tgagtccatt tggaaatgtt
    10381 tcatgtgtcc attcgggatc acaaaagata taaagatctc cctcatcaaa ggaaacagac
    10441 ttttgataag tctctaagat cttcgaattc gctggctgaa aattcagcaa ataagaatac
    10501 aaattgttct caaatccttc tggaatcagc aaatgacttt gcacagaaaa ttcttcagat
    10561 aggccaacga ttacttttgt atggtaaaac tggcgcttgc gactttgata cacagcttct
    10621 cttaaaattt tctcgtattt gctttgttcc ttgtctaagt cctcaaagat ccgccgagca
    10681 cttgctgttc ttcccacaat gcttccatca ttgaatacta gtgtcctaac atcagctggt
    10741 aaatctagtt cttcagcatg tgagattgtt aaatctgtga tgatcgttcc tggactccct
    10801 tttgctagtt gataagctgt cttcctatca aaaacttcat gaacattatt accgtaaaat
    10861 gtcgtttcta caatcgatcg aatactggat aacagtggat ttccacttgt gatttcgttt
    10921 aattcgagac tctcttttgt actcatggct cctcctcatt ccctaaacaa aaaaacgttt
    10981 tctttattat acattattcg agtttttatt tgtactgctg cgctgtggga agcaaacgag
    11041 ttatttttca cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt
    11101 tccttttatc ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg
    11161 aaccataacg agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt
    11221 cgccgtttaa gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg
    11281 tgtatgaacc gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg
    11341 ctgttgcagc agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg
    11401 tatgttcttg cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg
    11461 ttgttgctga agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt
    11521 tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa
    11581 atttttgtga gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt
    11641 gagcatttgt agttcctaca aacattgcag tagcaccagc agcaaaagtt gtacctaaaa
    11701 caattttctt aaatgaattc atattaaact cctttttttc tccgtttttt attctttcga
    11761 cgctattaac tttatcattt agaaatattt cataggtaaa agaaacatga catttaatga
    11821 caaaataacg aattatttaa tatttgtaac atttaaacaa atattttttt gatacaaaac
    11881 cataaaagcc ttgttgtata agcaaagtaa tcgtttttct atgttttttt atttttgaat
    11941 gaaaaacgca gattctgtaa cattcactgc aataatccat aaaaaaaagc aaaaaaattt
    12001 ttgattttta tcaaattctc ttaaaaaaat gtaaaaaata caaaaaaacc agtaattgtt
    12061 acaacaatta ctggacaaaa actttgaata atgttaacta tttttttatt ttaactttct
    12121 ccgcaataat ttctgggcat gttccaattc gtttataacg acttcttctg tttctttagc
    12181 taatatttca tctaatacat caagccattt ttccggttct tttgttccta gacgtccaat
    12241 agcccaagct gctgttcctc tgatgacagg gcggacatct gttaaacatt ctgaaatctc
    12301 aggcaatgcc gttcgatcac ctagattagc taaagcaatc aatgcgtttc tctgcaaagg
    12361 tttcttccca cgccatgaac cagctaaatg cccaaattgc tgtttgaagt ctttattaga
    12421 catcgaaagc attggcttta attttggata tatttcgtct acttttggtt ccatctcttc
    12481 atgaaaatga aaatcttttc cttgattgta agggcagact aactggcaga tatcacatcc
    12541 ataaatgaca tttcgcattt ttttacgata ttcttccggc atcatccctt ttgtctgagt
    12601 ttgataagac aaacatcgct gggcattcat tcttccgtca ccaagcaatg cttttgtcgg
    12661 acaagcagtc acgcagcgca tacagtcacc gcaaccaaat tctcctggtt tatcaggctc
    12721 aaatacaata ttagtaacga tctcaccaag atagacaaat gaaccgaact cttcagtgat
    12781 caacaatccg tttcgtccaa taaatcctaa accagctcgc tgtgctacag cgacatcgat
    12841 cagttctcca gtatctactt gcggagctag acgccattcc tcttcttttt gccatttttc
    12901 tgctcttgtt tggatgaatt cgatcaatcg gttcaaacgg tctctgagta tatcatggta
    12961 atcgatcccc caagaagcac gagcaaactg cccgcgtttt tcatccctag gcacttcttc
    13021 atggatccta gtaggatacg caagtgcaat ggaaatgatc gatttcggct gatcaaatgt
    13081 catttctgga taaagacgct catcgatatt tggatgttca aagccagaag tatggccagc
    13141 tgctttttgt tcttccaacg attcttttaa tgaatcaaaa ggttccgccg tggtgaaccc
    13201 gattttgtca attcctaatc gtttgctttc ttgtatgatt tcttctttta atgtcatggt
    13261 cttcctcgct tgctgttaac ggctgtataa cccataatat agtttttctt ttttcagctg
    13321 gacaaactgg atctgctgat cggcaatctt cttttcttcc atccattttt ttcttgcagc
    13381 aaatacttcc caataatttg ttgaaattaa ttttcttttg ttatctaatg cattaaaaaa
    13441 attcaatgaa actggttttg tacttaacag ttcatggagc cttacctctt cccatggcga
    13501 aaactcagac agatcgcggg aatacaatac ttcttcatag agattttttt gctgtgtgta
    13561 cgtgaatgtt tgcgggattt tcccaggcct agcaatttct actagacgat tataaaacat
    13621 attcattaat ttgctgttgg caataaattg acttttataa taggccgact ggatcttttt
    13681 gattactcct tctgctttga tgactgaaaa taatgcaggc catgctttga cggttatttc
    13741 gtcgttttgt ttaactaata cgccaggtgt gaagccgact ggagaaagga acacaaaata
    13801 accgataccg gtattttcat ctaaaggctg aaattcatca aacaaccatt tgcctatgta
    13861 cagctcttca tctggttctg ttgtctcatt ttgatttgct atgaataatt tagctaattc
    13921 tgcttcaaga aattgaaggt cttgatcctt gtcggtattt tcctgctggc tgatcaatcg
    13981 aaagacttct tctttaatcg tatatttatc tttacgtgta aaattcatag ccacaccttc
    14041 cttccttact ctaactataa cggaatttct tcaaaaaagg tatctgtacg acgaaaaaca
    14101 gcataatttt ttttcatatc tcttataaaa aatgcaaggt ctgctcctca agctagacct
    14161 tgcacttttc agctttcttt tgtttgtgac aaaccttcat ggatcgtttc aatattccat
    14221 ttcatcatct gatagtaaga atctccttgt tctccttctt ttgctatgga atcagtgaac
    14281 agtttatcat agattttcaa acccgtttct tttgctaccc gttccatgct tcttgagtct
    14341 acactggttt ccacgaataa aaccggtact tcttttgctc ttatctgatc aatgatcgct
    14401 ttcatttgat caggcgtacc ttgactttct gtattgatct cccatatata agctgctggc
    14461 aaatcatatg ctcgtgaaaa atacttgaat gccccttcac ttgttactaa taattttttg
    14521 ttctcttcga tagaagcaaa actttccttc gcttgtgtat ctaattcttt tagtttttcc
    14581 acatatgctt ctgcattttt tttatagagt gtcgcatttt ctgcatcttt ttcagagaat
    14641 atacgtgcga tttcctccac atattttatt ccgttagata ggtctagcca agcatgggga
    14701 tctgcttttg tatgttcttc accgctagtt aaataaagag gttctacatt tttgctaact
    14761 gcaaaataat cttttccttc ttctttttta gccgtttcca tcaagttatc gaaccagctg
    14821 ttacctgttt caagattcaa accgttgtat aaaataacat ctgcatcgct tgcctttttg
    14881 atgtcttctg gtagtacttc atattcatgc ggatctgttc cgactggtac gatactgtgg
    14941 atatctattt gatctgttcc gacctctttt gccatgtccg ccaagatcga attggtagcc
    15001 acgactgaca acttttcttt acttccttca tccgcttggt cagaagcctg ctttccgcaa
    15061 ccggctaata ccaagaaact aactgccaag attaaaaaga gtgatttttt tgttttcatc
    15121 tatctgcttc ctttctgaaa taaaattcct tttgttggag aaaataaaaa agcaaggata
    15181 aaaaatgcgg tacatgctaa cacgatcgta gctcctgatg gccaattgta actataactg
    15241 aaaaacacac cgacaactgc acttagcata ccgattcctg cagctgtaat gatcattttc
    15301 agtaaatggt tcgttagcaa gtaagccgtg gcggctggtg tgatcaacat ggcaatcacc
    15361 aagattgttc ctactgtctg taaactgact acagccacta atgtcaaaaa gaacatcaaa
    15421 agataatgga tcagcgatgt gttcagccca taagcttttg ccattgtcgg atcgaatgat
    15481 gtgatcttca attctttata aaagaaaaag acaaatatca agacgattcc actaacgagt
    15541 gctgtcagca acaaatcgct ttccctaaca gctaagacat tcccaaataa aatatgatac
    15601 aaatctgtcg cactctgggc atatgaaatc aagataatcc ctaatgcaaa aaaggagcta
    15661 aagacaattc caatcgctgt atcatttttc aatcggctcc gctgagtcac gaaaccaatc
    15721 actgctgctg ttagcatccc aaaagcagtg gctccaaaga tgtaactaaa accaaacata
    15781 taagaaatgg ctacacctgg aagaactgcg tgagagatag cgtctcccat caaagacatc
    15841 cctcgtaaaa taataaaaga accgatgact ccagaagcaa gtcctacaag catggaagtg
    15901 accaaagcgt attgcaggaa ttgataattc attaatccat caataaagga ttggatcatt
    15961 ttttctctcc tccttttact aaatgtctga tgatctcacc ataggccaat tgaaggttct
    16021 tcgatgtaaa gacttcttgt actggaccgg aagcgatcag ctgtttattt aaaagaataa
    16081 cctcatcgaa atattcttct actttatgaa gatcatgatg gacgatcaaa atcgtttttc
    16141 ctgaattctt caattcctgc aagatgtcaa agatctttcg ttcacttaac gcatcaatcc
    16201 ctacgaatgg ttcatctaaa aagatccatt ctgctccttg ggctagagct cttgcaataa
    16261 aaactctctg aagctgtcca cccgatagtt cgctgatctg tcttttggca tattcttcca
    16321 tccctacttt tttcaaagct tgtcttgcac gttctttttc ttttttccca ggtctttgtc
    16381 caattcgtaa agatggatat gttcctaaaa gtacaacgcc taatacatca attggaaagg
    16441 aaagatccaa ttcacttcgc tgttccacgt aggctatttt tttacggact gagtttaccg
    16501 actgtccaga aaaggtgact ttaccagtag tagctggtat caactctagc aaagatttca
    16561 taaatgttga ttttcctgca ccgtttggtc cgataatacc ggtaattttt tgtggattta
    16621 tcgtcaagtt gatagttttc aaaacagttt tcccttgata agcggctgaa aggtcttgaa
    16681 ttacaatggc cgtacgttcc aaatcttaca ctccttttta ggtttaccta actttttatt
    16741 taaatatagt ttaactaaaa tggaaagcat tttcaagtag aatgatgaaa aagttttttt
    16801 cgaattttcc tcgcatttta tgaaaatttt ctgtagaatc acatcaagag ttcaataagg
    16861 ctaacctaat aatgaaaaac atgtgttgaa acgtttgatt attttttctt gttgactcaa
    16921 caacgaaagg agtttttgta tggaagaaga aaagttaact cgaagtctta gtgcccgtca
    16981 tatccaaatg atcgcactag gtggaacgat cggtgtcggt ctgtttatgg gtgcctcctc
    17041 aaccatacgt tggacagggc cctcagtgat gggagcctat gcgattgcag gattcttttt
    17101 gtatttgatt atgcgtgctt taggcgaaat gctttacgtt gatccttcga ctggttcttt
    17161 tgcaaattac gcgagcgagt acatccatcc tgtggctggc tacttgaccg cttggagtaa
    17221 tatttttcaa tatattgtcg taggaataag tgaagtgatt gctgtaggat cttatatgaa
    17281 ttattggtgg cctgatttgc cggcaattat tcccggaatc attgtcgtct tgtttcttat
    17341 gctggcaaat cttatttctg tcaaggcgtt tggcgaatta gaattttggt tttcgatcat
    17401 caaagtcata acgatcattt tgatgatcat tgcaggtctt ggggtaatct tatttggatt
    17461 tggtaatcat ggtcaggcag tagggatttc aaatctttgg aaaaatggtg gattcttcac
    17521 tggaggcgtc aaaggatttt tctttgcatt atcgatcgtt gttgcttcct atcaaggaat
    17581 cgaactgatc ggaatgactg ctggtgaagc agagaatcct aaaaagacga tcattgaagc
    17641 tgtccagtcc actatcggtc gaatcttgat tttttatatt ggtgctattt ttatcattgt
    17701 gagtatctat ccgtggaatc aattgagtga gattggttca ccttttgtac aaactttttc
    17761 taaagtcggc attacatttg ctgcaggact gattaatttt gtcgtgatta cagcggcatt
    17821 atcaggttgc aattcaggga ttttcagcgc aagtcgtatg gtctatacac ttgccataaa
    17881 tggaaagatg tctaaaaaat ttctgaaact atctcgtcac ggtgcgcctt tttatcctgt
    17941 cgtagcaatc tcaggtggaa tcttaattgg tctgatctta aattatcttc taccaatgtt
    18001 catcaaccag tcagaaaata ttttcgtttt cgtatacagc tcaagtattt tgcctggtat
    18061 ggttccttgg gtagtgattt tgatcagcga gattcgcttc aaaaagaagc acgcagataa
    18121 aatgcatgat catcctttta aaatgccact atctccttat acgaattatg tgacacttgc
    18181 ttttttagct atcgtattga ttttcatgtt tatcaatcct gaaacgagaa tctcattgat
    18241 tattggactc gttttcttgc tttacatgac gatccattac tttgtacgcg aaaaaagaac
    18301 cggaaaataa aaaaagaacg aagcttcacc ttagtggaag cttcgttctt ttttattgct
    18361 tttatcaatt caattttagc gtctcaccgg gagtcaggaa ataattctcc atgctcatcc
    18421 cattcagcga caagatcgtt tctactggta cgccagttcg ttcggctatt tgatttggac
    18481 cttctccttc caaaactacg gttgtcgcag cctgtgaagt cgcgctgttg gtattagttt
    18541 cagaggaatc tgttgtgtct tgtgttgttc gagcttgcat ttcagaagct tctaccgact
    18601 ctgctgtctc agaatcagaa gatgttgtgt cttgtgtgct tgttgactct gcttgaagag
    18661 aggcgctagt ctgcaaataa atatcggaaa tcttagcgac atgattcgta taattagccg
    18721 ataattgatt gatcaaagta tctaagtcta caccgtcttt attttctgct gcctgatacg
    18781 ctgcacgtaa ttttctgatc ccttcgtcgg agattgcttg aagctcacgt gctttttggt
    18841 tagcaactgc tgataaacca gctattccct cttgatttcc ctgaatttct gtctggtatt
    18901 cttcaatcaa tgcaggcgtc ttttcttcga tctttttcga ataatcattt acgattgctt
    18961 gaagttgctc tggcaaacca tttgctgttt tttcttcaga agtttgtgtc tcttttgttt
    19021 tagttgatga ctcacttgtt tgtttcgtct ctgttttctt tgtcgttgtt tccgttatgg
    19081 tcgtactttt cgcttctgac gaattttttc catgaaaata tagacccgca actcctcctg
    19141 ctatgatcaa aaacacggca actgctgccc caatcatgat catccgctta cgagctgcct
    19201 gtttttgctg tcttcttcgg gtcatccgaa cattttctcc ccactcattt tcatcataca
    19261 tttttttcac caccaatttt tatgtataat agcgttatca aacgcttcat attcttattg
    19321 tagcaaataa ttgtcgctgt gcacattttt atctttgtta tttataagaa aatttcacca
    19381 gttgagatga ttccaagatt gtccgtcgat ttctccttgc agctgcattg ttcgttcgtc
    19441 tatctcagcc atcagtttct ttatttcata taagagcgca cggtcttgat attctgattt
    19501 ttcgcttaat tcatctaatt gttggatcag ccaatcattt gtcataatga agttcctttc
    19561 atttcttgca gtgtaaaggt caagtcttcg atctgctctt ttgcctcagt cagtgcttct
    19621 acgatatatg cttgtttttc agattcttct tcctgcattt tctgattttc ctgtactctt
    19681 tgaataaacc agtcaatccg ctgagtcagc gatggtttgc cttccaatga gagatactgc
    19741 tgataagcaa ttagcttctc ccaacgtttt tcctgggata aatacataaa ttgtgctaaa
    19801 acgaatggcg gaagataggt catatggaat ttatttgcga ttcgctgata cggcctcaaa
    19861 aattcggaaa tcgtatattc ttcttttcct ccagcttggt attctttttc cgccacacct
    19921 gttgtcaata cgattcctag ttctttccct tttaatacat gtgctgctcg ttccaagaca
    19981 ttatcttgcc aaatttttaa atgagcagga gaactatacc agtaaaacgg aaactggaaa
    20041 atgatccgat catgctgggc caagagctgt agttcagtct ctatatctat ttttccgtct
    20101 ggataacttg attccaaatg atgccaagta gctggtaaac cagcagaagc ttctttgaaa
    20161 aattgctgat ttaaagattc ttcgatcgtt ggatggctaa tgatgataag tgtacgcatc
    20221 ccttttcctc ctttttagtc ttcgctttac agtgtagcat aaccgccaca agtttctttt
    20281 gccgatgctt gtcattttaa ccagactaat caggttgtcc aagaaactgc ggtttgtttt
    20341 tccaactgtt ttttcaaggt atccaaagca atattcttaa aattttcgtt tttcatacgt
    20401 ttacgatagt tcgtagccgt ttctccggta ctctttttga acattcggct aaatacgact
    20461 agactattga accctacttg ataggcaata ctttccacgg attcatccgt cagagaaaga
    20521 agaaacgccc ctcgattgat ccgtaccccg atcaaatatt ccttgatact ggtacccata
    20581 tgatttttga atactcgcga caaatggctc cgattgaccg ctaaatgctg ggcgatttcc
    20641 tcaacagaca atttttcagc ataatgtgcc tcgatgtatt tgactgtttc aacagctaaa
    20701 gaggaatgaa ccttttgtac accaccgctg acctttccgc catcttttaa aaggactgat
    20761 aaaaaacggt aggttaatgc tgttaactgt aattcatttt ccaaggtttc ttccgtatct
    20821 ttcatgcatt ctaaaataat gtcgatatat ttttgtatgt cagaagagac catcgtgtat
    20881 tggtcttctc tgaaaagcga ctgccgaatg atttcttctg cagcactccc accaaatgaa
    20941 agccagcaat acatccacgg atcttctcca tctgataagt aaaacgtcga gtctcctgga
    21001 cgaatcaaga acagatcacc ttttttcaat tggaattgtt gatttttcac atgataagat
    21061 cctttgcctt ccatcacgac atgcaggatg taatcattgc gaatagcagg accaaaagaa
    21121 tgaaacggca tcgttttaga gaaaccacaa aaatcaaaat acaattcttt ttctacttgt
    21181 tgatgttcct tttttaaaaa aatcgattgc tccacgtcta tccctcctta ctcctttagt
    21241 ataatgcaac aaaaagttaa ataaaagtca cagaaatata ttaaaaagga agcgatttct
    21301 tgttaagttt aagtcattaa aagatctttg ttattcgttg taaccggttt acttagtatt
    21361 atatattgaa caggagagga atacagatta tgaaaagcaa aatgatccaa cgtgtgtcct
    21421 acgctttagg cgcttttgga cacgacacat tctatgttac attaagcact tattttatgg
    21481 tattcgtgac aagccagatg ttcgcaggaa cagataaggc aacgaatgca aaaatgattg
    21541 ctacagttac aagcttagtt gtaggtatcc gcttagttga aattattttt gatcctttga
    21601 ttggtggcat catcgataat actcgtacaa aatacggtaa attcaaacca tggttagtta
    21661 tcggcggcgt tgttagttcc gtactcttgg tagcaatctt taccaatttc tttggcttag
    21721 ctactcataa tacgacttta tttatcatat tatttgcaat cgttttcatc ttattggata
    21781 gtttttattc atttaaggat atcgcttttt ggtcaatgat tccagctttg agtacggaaa
    21841 caaaggaacg tgaaaaattt gctacgtttg ctcgtttcgg ctcttctcta ggtgcaaatg
    21901 gtactactct agtcgttgtc cctatcgtta ctttctttac ctatcttgtt actcataaac
    21961 atattgaagg cgcttcaggc tggttctggt tcggtgtgat tgctgctgtt atttcaggcg
    22021 gtactgcgat tatcactgcc ttgggcacaa aagaagtcga tactgcgatc cgtcagcaaa
    22081 atgaaaaagc tgggatcaaa gatgtgttca aagcagttgc tcaaaatgat cagttgatgt
    22141 ggcttgccct tgcttatatc gcttatgcga ttgccaatgc agcaacaact gccgtattat
    22201 tctacttctt caaatatgta cttggccaac caactgaatt ttggatcgtt ggtctagttg
    22261 ctgccttact tggtttagta gctgttccac tattcccaat gcttacacgc gtcatctctc
    22321 gtcgctacgt ttaccttgga gggatcgcaa tgatgatttc ttcttacttt ttctttacga
    22381 tcgcaggttc aagtttgcca atcgttatca tcgggctagt attgttctac ttccctcaac
    22441 aattgatttt cttatccgct ttgatgacga tcacagactc tgttgaatat ggtcaatgga
    22501 aaaatggtat ccgtaacgaa gctgttactc tatcaattcg ccctttacta gataaaatcg
    22561 ctggtgcttt gtctaacggg atcgtagggt ttgtcgctat cgcttctggg atgactggta
    22621 atgctactgc tgctgatatc actgctcatg gcgtaacgac tttcaaagct tatgcattct
    22681 atgctcctgc tgctttgatg attttctccg ctattatttt tgcttggaag attacattga
    22741 cagagaaaaa acatgcacaa atcgttgaga aattggaaaa caaattggct ccagctgata
    22801 caatggaaga tgaacttcac gatgcagtag atccttccca ttaagaaagg atcttgaaaa
    22861 tgaagattac agaaaaagat tttggccaag gttatcactt gataaccttg gagaacaaaa
    22921 acaagctagc actttctatc tctgatttag gtgctagaat cgtcagttta aaatcaaatg
    22981 atcgagaatt agttttggga tttgacaccg ctgaagaata tatcgaaaaa gatccgtata
    23041 tcggtgcttc tatcggtcgc accgcaggga gaatcgaaaa tggccgcttt tctctcaatg
    23101 gaaaaactta ccaattagcc acagatccaa aaactggaca taatctgcat ggtggtgctc
    23161 ctggatttga attaaaaaaa tggtcttatg tcattttgaa tggcgaaaat gaagcttctg
    23221 tcatttttac tactacaagt cccgacggag aacatggttt tccaggtaca atggatgtgg
    23281 aaatccgcta tactttgaca aaagataata tctggcgagt gacctctcgc ggcaccagtg
    23341 accaagatac tttattcaat ccgacaaatc atgtttactt caacttgacg ggagatgctt
    23401 ctcagtcaat cgatcaacat gagctttggc tcaatagcga agcttatgct cctttgcgaa
    23461 cagatagtat tcctataggg gtaaaagaaa atgctgcagg aagtgctttc gattttcaaa
    23521 tacctaaaaa attagcgtcc gtttttgcta gtgatttgga tcaaaaaaat cttgttgacg
    23581 gaatcgatca tccatttttc ttaaaagaaa ccggcttggg taaagaagcc gctcgcttga
    23641 ctagccccga taaaagaatc caagtagata ttgctacaga tgcttctagt gtggttattt
    23701 tcactgcaaa tttcggcaca gaaacaccag aaatgcgcaa ccgaaaactg gcacatcacg
    23761 gtggcatcac atttgagact caaacagctc caggtgcaga gcgcttctca gcatttggtt
    23821 cgattcattt aaaagccgga tcagtatttg aaacagtaac agaattcaaa atcaaaacaa
    23881 gaaaggaata actcatcatg gaaatcaaaa gaagcttaaa cacaaaattt gccgaaacat
    23941 tcggacctaa aaaaactaca caatacttct caccaggacg catcaatttg attggtgaac
    24001 acacagatta caatggaggc catgtcttcc ccgcttccat cacttatgga acgcaaggtg
    24061 tagcagcacc tcgagacgat aataaaatat tagtttattc cacaaacttt gacgatatcg
    24121 gtgtcattag ttttacttta gatgagctag aatatgataa aaaagcaaac tgggcaaatt
    24181 acgtaaaagg aatggtcctt aaattaaaag aagctggaca ctcaatcgat catgggtttg
    24241 agttattagt ggaaggaaca atccctaatg gtgcaggact ttccagtagc gcttcattag
    24301 aattattagt aggtgttgta ttagaagatc tgtttgattt aaaaacagat cgcttggaac
    24361 tagtccaaac aggtaaaaaa gtagaaaatg aattcatcgg tgtcaattca ggtatcatgg
    24421 atcagtttgc catcggcttc ggtgagctag atcatgcaat cttattagat acaaatacgc
    24481 tgaaatacga aatggttcca gtgaaactag atggttatgc aatcgtcatc atgaatacga
    24541 ataagcggcg ggaattggca gattcaaaat acaatgaacg ccgtagcgaa tgcgaagaag
    24601 ccttggcacg tttgcaaaaa actttgccta tctcttcttt aggagactta gatgaagaag
    24661 aatttgaatc aactattgac caaatcggcg atgatacatt gatacgcaga gctcgccatg
    24721 ccgtttctga aaatcagcgg actttgaagg caaaagctga attagaagct gggaacttag
    24781 aagcatttgg tcaactgtta aatgattctc accactctct tcgctatgat tatgaagtaa
    24841 ctgggatcga attagataca ctagtagatg ctgctcaaaa acaagaaggt gtcttaggtg
    24901 cacgaatgac aggagctgga tttggtggct gtgcgattgc tcttgtcaaa gaagaaaata
    24961 tacctgcctt caaaaacaat gtctatgatg aatacatgaa agttgtcggc tatgctccgg
    25021 aattttatgt tgcacacatt ggaaatggga caacaaagat cgatccagaa gctgttcatg
    25081 tttgataaaa aaatcaacga tctcagctaa aaacaaatag ataaaaataa cctctcagca
    25141 atttccaaat gtgagccttt tgctcgtatt tgaaaattgc tgttttttat tgacacggaa
    25201 agagaacgtg tgttctaatt gtgttgaaga ggtgattgtt atgtgggcga atcaaaatcc
    25261 ggtatttgtt tatgaaaaag aaccttcaag agatatttta tgtattgact gcaaatcttt
    25321 ttacgcctcc gtagagtgcg ttgaaaagga tcttgatccc cttacaacta aattagtagt
    25381 catgtcctat ccgtccgatt cgacagaaac acggggtagc ggattaatct tggcttccag
    25441 tcctgcagca aaaaaagctt acggcatcac caatatcagc cgtgccagag atcttccctt
    25501 cccctatcca agtgatttat atattgttgc cccaaggatg gcctattata tggaaaagaa
    25561 cagagaaatc aattctatct ataaaaaata tgcagacgaa gcgaaccatc atgtctattc
    25621 cgtcgatgaa agcttccttg atgtaacaaa tagcatggca ctctttggta aaacaaatgc
    25681 gtacgacatg gctaaactga tccataaaga tgtgtttgag cagaccggaa tctacacgac
    25741 tataggaatt ggcgataacc cgctacttgc taaactagcc ttagacaatg gctcaaaaga
    25801 cggaaagcaa atgtttgctg aatggcgtta tgaagatgta ccagaaaagt tatggaaaat
    25861 ctcagatatc actgactttt ggggaatcgg caaacgaaca gcaaaacgtc tgaaacaaat
    25921 gggtatacat tctgtttatg atttagctca cgcggattat tatcacttga aaagcaagct
    25981 aggggtcatc ggtactcagc tttatgccca tgcttggggc atcgatcgaa gttttttggg
    26041 acaagaatat acaccaaaat caaaaagtat cggaaatagt caaatcctag atagagatta
    26101 tacaagacga gaagaaatcg agatcgttat caaagaaatg gccgaccaag tcggcactcg
    26161 cttgagacga tctgcagcaa aaacacaagt agtcagctta tgggtaggat actctttagg
    26221 ctatcttgat ccaaatggca agaccgggtt tcaccagcaa atgaaaatcg aagcgacaaa
    26281 tgctagcaga gatctagcag atgctttatt gaccattttc caccagcatt accactacca
    26341 ggatattcgg cgtgtaggcg tgaactgttc cgacctcgta tacgcgacag gtttacagct
    26401 taaccttttt gaagatcctg aaaagcagat aaatgaagcg aaagttgatt ttgttgtcga
    26461 tacgattaga aaaaaatacg gctttcaatc aatcatccat gcgcatagtc tcttgcaagg
    26521 aggacgcgcc atcgcacgta gttctctcgt tggcgggcat gcaggtggta tgagtggatt
    26581 agaaggtgga aaagatgaga cggacagcaa aaaaattcat tgactataac gagtatcgag
    26641 atcggccatt tggattaaaa tgggggactg cttttgcgat ggaagaacta gtcaacggta
    26701 tcaaaaaaat gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca
    26761 agaaatcgat agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct
    26821 caatttaaaa gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga
    26881 agcctatgaa gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta
    26941 tattgagtta aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat
    27001 caacttcaaa aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt
    27061 tggcttattt ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg
    27121 aaaaagtcgc ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc
    27181 tatccatcta ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct
    27241 ttttcttctg accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc
    27301 aagttctcca taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt
    27361 tgcccttcat ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca
    27421 ctcatttgac cggcattctc taggataaca tgtttggcat tttcaagaaa aaagcattct
    27481 tgctgtctta agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc
    27541 atgttgatga cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc
    27601 ttttcatttt tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg
    27661 gcaaatagga catgccctac atggacattg tcatcatagg ttgattcttc taagatccca
    27721 cctaaaacaa gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca
    27781 agcaaacgta ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa
    27841 aaaatcattt tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa
    27901 cacgcatgga atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact
    27961 gtagtgaccc ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg
    28021 ctctaaaatg atatttttac acaaccgtta cggttgtttt acgaaattgg acg
//
LOCUS       DACERL010000032        27245 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.033, whole genome shotgun sequence.
ACCESSION   DACERL010000032 DACERL010000000
VERSION     DACERL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27245
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.033"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            342..1028
                     /locus_tag="IXF54_001433"
     CDS             342..1028
                     /locus_tag="IXF54_001433"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1404706.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            1048..4629
                     /gene="smc"
                     /locus_tag="IXF54_001434"
     CDS             1048..4629
                     /gene="smc"
                     /locus_tag="IXF54_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1404707.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPNQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4638..5447
                     /locus_tag="IXF54_001435"
     CDS             4638..5447
                     /locus_tag="IXF54_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1404708.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5459..6457
                     /gene="ftsY"
                     /locus_tag="IXF54_001436"
     CDS             5459..6457
                     /gene="ftsY"
                     /locus_tag="IXF54_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1404709.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6644..8911
                     /gene="gshAB"
                     /locus_tag="IXF54_001437"
     CDS             6644..8911
                     /gene="gshAB"
                     /locus_tag="IXF54_001437"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1404710.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8999..9451)
                     /locus_tag="IXF54_001438"
     CDS             complement(8999..9451)
                     /locus_tag="IXF54_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1404711.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            9601..10551
                     /locus_tag="IXF54_001439"
     CDS             9601..10551
                     /locus_tag="IXF54_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1404712.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10544..11503
                     /locus_tag="IXF54_001440"
     CDS             10544..11503
                     /locus_tag="IXF54_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1404713.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTFLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11500..12255
                     /locus_tag="IXF54_001441"
     CDS             11500..12255
                     /locus_tag="IXF54_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404714.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12278..13231
                     /locus_tag="IXF54_001442"
     CDS             12278..13231
                     /locus_tag="IXF54_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1404715.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13875..14171)
                     /locus_tag="IXF54_001443"
     CDS             complement(13875..14171)
                     /locus_tag="IXF54_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1404716.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14527..14883
                     /locus_tag="IXF54_001444"
     CDS             14527..14883
                     /locus_tag="IXF54_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1404717.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14893..15792
                     /locus_tag="IXF54_001445"
     CDS             14893..15792
                     /locus_tag="IXF54_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1404718.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGKLLDHYSNIKQVVF
                     GHTHRRFEDQLIHGAIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            16309..17040
                     /locus_tag="IXF54_001446"
     CDS             16309..17040
                     /locus_tag="IXF54_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1404719.1"
                     /translation="MMKKEIVLTATTMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            17185..19212
                     /locus_tag="IXF54_001447"
     CDS             17185..19212
                     /locus_tag="IXF54_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1404720.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            19320..20417
                     /locus_tag="IXF54_001448"
     CDS             19320..20417
                     /locus_tag="IXF54_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1404721.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMRQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVIIETKITKKGHSKVLYREEKEALQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            20723..22191
                     /locus_tag="IXF54_001449"
                     /pseudo
     CDS             20723..22191
                     /locus_tag="IXF54_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(22284..23018)
                     /locus_tag="IXF54_001450"
     CDS             complement(22284..23018)
                     /locus_tag="IXF54_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1404722.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            23344..23754
                     /locus_tag="IXF54_001451"
     CDS             23344..23754
                     /locus_tag="IXF54_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1404723.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            23747..24448
                     /locus_tag="IXF54_001452"
     CDS             23747..24448
                     /locus_tag="IXF54_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1404724.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            24448..26061
                     /locus_tag="IXF54_001453"
     CDS             24448..26061
                     /locus_tag="IXF54_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1404725.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHTFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            26051..26272
                     /locus_tag="IXF54_001454"
     CDS             26051..26272
                     /locus_tag="IXF54_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1404726.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDECDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            26269..27030
                     /locus_tag="IXF54_001455"
     CDS             26269..27030
                     /locus_tag="IXF54_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1404727.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPEIFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
BASE COUNT         9097 a         4651 c         5648 g         7849 t
ORIGIN      
        1 aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc
       61 tctaatattc aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc
      121 cagtttctcg gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt
      181 ttttcttcat actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta
      241 ttaaagcttt acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat
      301 aaggaagtat cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa
      361 aagaactaaa agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt
      421 ttacccattc atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac
      481 ttgaattttt aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat
      541 atcctcattt gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca
      601 gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag
      661 aagagaattc aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt
      721 taggcgcgtt atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga
      781 tttttcctaa agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac
      841 aagaagtctt gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag
      901 gtccagctca tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca
      961 ctggacaagg aaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag
     1021 cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt
     1081 aaatcatttg cagacaagac agtcattgac tttgaaaata gcgtcacagc agtggttgga
     1141 ccaaatggca gtggaaaaag caatattaca gaagctatcc gctgggtatt aggagaacaa
     1201 tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc
     1261 cgtaagcagc tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg
     1321 ccattggagt acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag
     1381 tttttcatca ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagactct
     1441 ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt
     1501 agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag
     1561 cagcggaaaa agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc
     1621 caagacatca tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca
     1681 gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt
     1741 gccgaaatca agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc
     1801 aatctagaat taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag
     1861 cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta
     1921 gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca
     1981 aagcatacac aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag
     2041 gttaaacatt ttgaaaagct tcaagaaagc ttgttgaaag aagtagcaga aaaagaaaca
     2101 gaaatccaaa aagctgaagc aagtctcata aaaacacagc aagaattaga aaaataccaa
     2161 aaatcaacga aagaattact ggcagaacta agagatcaat atgttgattt gatgcaagag
     2221 caagcagctg taggaaacga actgaagtat ctagaacgtc aatatgtaca agaaacagca
     2281 aaaagtaagc agacgttagc caagcaatcg gaagtagagg catcagtaga ccgattgatc
     2341 ttacaaaaag aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca
     2401 aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa
     2461 gaacagccta aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa
     2521 agccttcaag aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg
     2581 cagcataaac agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct
     2641 gcagatttca cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt
     2701 gaaaatgaaa aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga
     2761 gcaacattcc ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca
     2821 caagcagcag ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccaat
     2881 caaatccaaa ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact
     2941 agtgccaatg caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt
     3001 gatgtgatga atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc
     3061 agtttgtttg tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat
     3121 aaacaactcc aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta
     3181 gccgaagaac aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa
     3241 gcgacaaatc agctgcaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa
     3301 atctcaactt ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg
     3361 aacgaattga aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa
     3421 atcaaatcgt taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt
     3481 caaaaagcac aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag
     3541 atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata
     3601 gaaaagcagt tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt
     3661 ttggaaaaac aaattaacga attatctgct cagcgagaaa cattgaaagc cgagttggca
     3721 aaagccaaag agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg
     3781 gcagagcgta atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa
     3841 aaagaccgag ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag
     3901 ctgacatttg agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga
     3961 acaaaggttt catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca
     4021 atcgaacaat acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat
     4081 ctgttagcag cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg
     4141 acaagattca aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat
     4201 atgtttggcg gcggtaaagc tgaattagta ctgacagaac caactgattt gctgaaaaca
     4261 ggtattgaga ttgaagtaca acctcctggc aaaaaactgc aaagtttaag tttgctttct
     4321 ggaggagaac gagcacttac agccatcgcg ttgctctttt ctattatccg tgtctgtcct
     4381 gttccgtttt gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt
     4441 tttggtcgtt acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc
     4501 aaaggaacga tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta
     4561 tcaaaaatcg tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg
     4621 aaagtgtagg aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa
     4681 atgaacaaaa gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag
     4741 gggtcaagat cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag
     4801 aattaggact ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa
     4861 aaaatgatac tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct
     4921 tatacaaact ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt
     4981 tacagctgcc tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc
     5041 aattccaacc agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa
     5101 tcgcttatga ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg
     5161 aaatgtatga gatcatcaaa acgagaagta tgcttttgga atttatgcca aaaggtgtta
     5221 caaaagctta tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca
     5281 tggcaatcgg tgatgaagaa aatgatttac cgatgatcca atacgctgga ctaggcgtag
     5341 cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg
     5401 aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt agagtagggg ggagtcaaat
     5461 gggatttttt gataaaatca agcgtgcttt tactggcgaa gagaaaacag aagaagttga
     5521 agtacaggag aagtacgaca agggtttaga aaagacgcgt aaaacatttg gtgaacggat
     5581 gaatgagttg tttgccaatt ttcgaacagt cgatgaagac ttttttgaag aattagaaga
     5641 aacacttatc ggtgcagatg taggttttga aacagcgata cgaatcaccg aagcattacg
     5701 tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga
     5761 gaaacttgtg gatctctatg aagcggaagg aatcaatgaa gtaaatgaac taaatatcca
     5821 agaagacgga ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag
     5881 tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc
     5941 agataccttc cgcgctggag caatcgacca gttagttgtc tggggcgaac gcgctggtgt
     6001 tgaagttgtt cgtggcaatg caggcggcga tccagcagct gtcgtattcg atgctttgga
     6061 aagagcaaag atagagcagg cagatgtttt actagttgat actgcaggcc gactgcaaaa
     6121 taaagtgaac ttgatgaacg aattagaaaa aataaaacga gtaatccagc gggaattacc
     6181 agaagcaccc caagaaacac ttcttgtact agatgcaaca actggtcaaa acgcgatggt
     6241 tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt cttgttttga caaaattaga
     6301 tggtacagca aagggcggta tcgtacttgc tatccgtaat gaattgcatt taccagtgaa
     6361 attggtcggt cttggtgaag gaatcgatga cttggagccg tttgatccaa atgattttgt
     6421 cgttggattg ttcaaaggat tactaaaaga agaataaccc atgtctaaaa agcaggagct
     6481 gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat
     6541 gccttcattt ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga
     6601 ctttttgatt ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca
     6661 attattattg catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg
     6721 ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt
     6781 tggcgatcga tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat
     6841 gatcactcct gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga
     6901 tgtgactgct cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc
     6961 cttaccggaa aaagacgaag cgatcattat tgcaaaatta aaaaatttcg aagatgtctt
     7021 gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa gcggtatcca
     7081 tttcaatttt gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt
     7141 tcaagatttt tccgaattca aaacagaact ttatttgaaa acggcgagaa actttatgcg
     7201 ctatcgctgg atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt
     7261 tttagatgaa tcacatccgc aagaacctgt tcgcagtatt cgtaacagtg cgctgggcta
     7321 tacgaatcat ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat
     7381 cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg
     7441 tttccgaggc ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt
     7501 gcggaatatc gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt
     7561 cttacaatta ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg
     7621 ggtagcagaa ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac
     7681 agaatttcat caagaaggta gagaaattct tgaagggatg aagcaaatgc tggttgaatt
     7741 ggattggttg gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga
     7801 acaaacgtta gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc
     7861 tgtggcattg ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg
     7921 ttttcgcgaa atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt
     7981 ccaagtcaaa gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt
     8041 agaatatgtg aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat
     8101 ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg
     8161 cgcagaattt tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc
     8221 atttgtgatc aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg
     8281 agctagtctg gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt
     8341 tttagtagaa gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt
     8401 ccaagccatc atgttgcgag tccctgctaa tgttattggt gacggtatcc gaacagtgaa
     8461 agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt
     8521 agaattgatc caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat
     8581 cgaatcagtt ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac
     8641 aggtggtgat tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt
     8701 ttctgcggta gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa
     8761 agaaatcgat cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc
     8821 agccatgcat atgcatgttt atccctttgc aggaaaaggc agaagattga cgatgaatgt
     8881 attgaaattg ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat
     8941 ttgatgtcaa tctgatcaaa agcttttttg catgtaaaaa tttatgtgtt ttttcgtttt
     9001 aaaataaacg atcccagaag ctttttttct tttttggttt ttctttttca actggcgaag
     9061 agttttcttc tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg
     9121 ctgttaagag caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtaa
     9181 atcggctatt gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag
     9241 ccccattcaa gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt
     9301 tgttttttgg ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg
     9361 tccctaaata tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat
     9421 ccaaatggct ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct
     9481 ttcttacgat tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt
     9541 tgttcaacga ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg
     9601 atgcgctacg gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc
     9661 ggagtatcga atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc
     9721 ttgctagtga ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt
     9781 atttgtgggt tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg
     9841 ggcacgatgg atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca
     9901 tccttgattg tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt
     9961 ttatccctta cgcgattgtt cccccagaaa aatcaattga tgcttccgtt gattggagtc
    10021 atgttaggga atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta
    10081 caaaatatga cttcatggct tcaaggaaat tttgcgactg tgatacgagg aaattatgag
    10141 ttattgtatg ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg
    10201 atcattggtc tgggcgaatc gtttgcagta aatttgggaa tagattacca gaagatgcag
    10261 tttttaggca ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc
    10321 attcctttta tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg
    10381 cacaaaacaa ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt
    10441 gtcgctcgcg tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc
    10501 ggtggggcta tcttccttta tcttctgata agggggcgac gatatgctta aaaaaaagcc
    10561 atctattttt attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat
    10621 ccatacatat ggaaactggc attttgcatg ggcattgagg agtaagaaaa tttttgcatt
    10681 tatccttgtc tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa
    10741 ccagtttttg actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacgtt
    10801 ccttttcttc tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt
    10861 attgaacgtt gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa
    10921 aatgagagcg aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg
    10981 tagtgtcagt acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg
    11041 cagattattt gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat
    11101 cattatcggc tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct
    11161 cggaaatgaa caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt
    11221 atgttgtgtc agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt
    11281 agggtttatt gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct
    11341 atttatcgga agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga
    11401 gcagattttc ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta
    11461 ttttgtcaca aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc
    11521 aataaactgt accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag
    11581 aagttgactg cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc
    11641 agtcgtttga taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct
    11701 tggaaatcca atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat
    11761 gtccaaatca ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa
    11821 ttgacgacaa aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa
    11881 atagcggatc aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca
    11941 atggtgctag cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat
    12001 atgaattttg cggttcaaat catgcagatc ttaaaaaaat tagtacaaga aatcgggaaa
    12061 acaattgtta ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattatt
    12121 gcaatgaaag atgggcagtt attcacgcaa ggaacgaccc aagaaatgat aaaaaaagat
    12181 gtactggacg aattgtacga aatggacata cgaatttgtg agattgaagg tcgcagattc
    12241 tgtctttatt tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat
    12301 cttgattcta gcatcatttg gtttagcagc atgtggaaat agtgacaaca ccagtcaggc
    12361 agatacaaag aaaagcagta cgcaagctga aacggcatta acgataaaag acagtaatgg
    12421 cgatcaaatc gaggtgccaa agaatcctga aaaagtcgtc gtattcgaca atggttcttt
    12481 agatacgatg gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct
    12541 tcctgagtat ctttcatcct ataaaaaagt ggaatcagca ggtgggatca aagaaccaga
    12601 cttagaaaaa atcaatcagc ttcagccaga cctgatcatc atttctggcc ggcaaagaga
    12661 ttttcaaaaa gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa
    12721 tccatgggaa tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga
    12781 agaagctaaa actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc
    12841 agaagctact gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta
    12901 cggatctggt tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga
    12961 tcaaatcgaa gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa
    13021 tccggatatc ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga
    13081 tgatatttct gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat
    13141 ctcccttgaa ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat
    13201 gatcgaagat gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata
    13261 ctatgtaaca ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt
    13321 atcaaaaacc atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttggg
    13381 aaatcttttt tcaacgtgaa ttcattctct acatcatacg ggttgattct agaatgggga
    13441 gtggcaaaaa atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat
    13501 caaaggtaca aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga
    13561 tctcttcttt aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa
    13621 aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca
    13681 caatctcttc taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga
    13741 aaatatgaga agctattttc tgtccagcgt tcttattact taaagcaaat cgtagttgtc
    13801 acaatctctt ctaaacgatg ttccatcagc tgaccttcgg tattagctca aagcactgat
    13861 ttcacaacct ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt
    13921 tttcgataaa aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc
    13981 tcttctgctt tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa
    14041 gctaattttt tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt
    14101 acttcaatat aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata
    14161 tattccttca tagtgattcc tccaaagaag tctttttatt tgataagttt actctaccga
    14221 agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt
    14281 tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg
    14341 ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct
    14401 tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt
    14461 cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg
    14521 atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa
    14581 gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa
    14641 gtcagttgcc aattggacac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca
    14701 gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac
    14761 agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt
    14821 ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac
    14881 tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa
    14941 ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg
    15001 cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt
    15061 ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg
    15121 cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt
    15181 gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc
    15241 gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc
    15301 atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat
    15361 ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct
    15421 aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgcg
    15481 tttttaggat cagaatcatt cggaaaatta ctagatcatt actcaaatat caaacaagta
    15541 gtttttggtc atacgcatcg gcggttcgaa gatcagttga tccatggagc aatttatagt
    15601 tgtcgaccat tcggttatta ttatgaatgg caactgactc gtgattttgt tttgaaagaa
    15661 cagttgttag acagctatga tcctatgaaa ttacgtgtac tcttgcgtgc ccatcatccg
    15721 gcctttcaag cctatcaaac acaacatttg caagaggaat ttgaacaggc gatgacaatc
    15781 gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga
    15841 taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgtg gaaaataatc
    15901 agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgttta
    15961 ttcttttttt gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata
    16021 attaatattg attgaagtct aggcagtcaa gtcacatttt gatagatagg agggggactt
    16081 acgaattatt ttaaaggcta tgacagctgt atttctagat tgtgtttgca tcaagttgca
    16141 ataaaaacta gaaatcaaat cgggctgata atcatttata actaatcgat acataggacc
    16201 aaatataggt ctatgttctt ttatttcata agtatcgtaa aacagaattt tgctatggat
    16261 taatccaatc gactgatatg tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa
    16321 gaaatcgtat taacagcaac aacgatgtta ttcagcatgg tagcaagtac gacttttgtt
    16381 tctgcaactg aagtataccc aaaggaatat aacacagaag gaacgattac atttgaagca
    16441 ggagatgagg gtgtaactcc tcctgttgat ccagagaatc ctgatcccaa taaacctgtc
    16501 gatccatcag atcctccaag ccctggaaca ggaggggcat tatcgattga ttatggatca
    16561 aaattcaaat ttgggactca aaaaatttct actgcggata aaacgtatta tgcagctgct
    16621 gatgtgatga acgatgggtc tagaaaaccg acatatgtcc aagtgacaga taggagaggt
    16681 accttatctg ggtggaaatt aagtgtctct caaccagaac agtttaaaac tgccagtggg
    16741 gatgaactag ttggagcaca gctgaaattt acgaaagggc aggctgtttc tttggttgat
    16801 ccaacatata cgccgcaaac ggtgaattct gaattaacgt tgacaccggg aggaaacaat
    16861 acgttagcaa ttaatgctaa atcaggaaca ggtgtgggga cttgggtcta tcgatttggt
    16921 gcaaatgaaa atgaaaatca agatgcggta cagctttcgg tacccggtaa atctgtcaag
    16981 ttagcgcaac aatattccac taaattagtt tggacattag aagatacacc taacaactag
    17041 tttactgaaa agaactgtta aaaataaata cgtagtcata ttgtcttgcg aggtaatcga
    17101 gcaaaaattg tcaaaggcta ggctaaattt gtttggatac gaatagttca tctaggaatt
    17161 taacgatcag gaggaaaaga tatattgaaa aagaggagga tcgtgctcat tttaagcagt
    17221 ctcttagtga ctaatatctt tccgatcata gggtactctt tggaattgaa tcagttggag
    17281 catggaaata gaagagacga agaaagcaaa ctcacttcga cattcgacag tttaatagaa
    17341 tccaaccaga cacaagaaag cagtgaggat ttaagtattt cgagtagtgg agagaccact
    17401 gacaacgtag aagctgaggg ttcggacaaa caaggactgg aagatgcacc aatttctgcg
    17461 gttgagctgc ctgacgatcc caatattatt tctatcgata aaatttttca aacacctatc
    17521 ggtactagca cgtccatatt ggaaaatggg aagttactgc agttgaaccc agcagaaagg
    17581 tctcaaagag gagctatttg gtcaaataag aagattagtt tactctcgga ttttacattt
    17641 aaatcttatt tatacttagg aaatcaacga gatagagcgg gagatggcat gacctttaca
    17701 ttgactggcg atcctagaat ggctagtaat cccagtcaag ttattggatc gccaggtatg
    17761 ggaattggct catattctac tcgttctggc caaccttaca ttgcgaatgc gctctcgatt
    17821 gagtttgata cgtataaaaa tagtggttct tctgatcgaa tggatcgaga gataactgct
    17881 gataatagag ggcatggaca tgtcgctttt gtcacgccta aaccaaataa taataattat
    17941 tctggagagc atactggcgt gacggtagct cctacatttc tttctaatgg cacatggcgc
    18001 ttgttaataa ttaaatggga tgcggagcaa agaagtctta cttatgattt ggaaggtgtc
    18061 ggtcgctctt ctcggattat acctgatctc attaggcagt ttggttctac agaagtaaat
    18121 tgggggttta cttcttcaac aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga
    18181 cttccatcga atgtcacatc aacagctgat gtaaaagtca acgatagtga ttatggggcc
    18241 atcgctgaag ctggaggaaa cgataagata actatcagaa atacattgaa ggtcgagggg
    18301 gattttcttg aatataagaa aatgcagact agtatcgacc tcccacaaga attaactatt
    18361 cccgagcata tcttgatcaa tggaaatcaa attgatgcca aagatatctt gataaccgga
    18421 aatcgagtca cgctaaatct gacagattat tttattgctg gagatacgtt aataatcgaa
    18481 ttggagacaa ctttgcagga agtaattcct gaaaaagtac tcactatgca ttttgaatac
    18541 cttgaaaacg agaatttgat tgagcaatcg aatgaaataa acctaaagat cgcaaagctg
    18601 aaggagatag atatccatgt ttattataaa gataaagaca gtcagattga tattgctgaa
    18661 atgaaaacaa tcactgggaa aataggagag aagtataaag aaactcctat tgatattgaa
    18721 gggtatgtct ttgactcgga ttcagcgaat acagaaggca tcttctccga aaatacagag
    18781 gatatctatt tttattacag aaaaggcaaa ctttatcttt caactgctcc tcaatatgtc
    18841 aattttggaa aacataaaat atccagtaga ccattaagtg aattcggaca gttttcaggt
    18901 ggattgaaaa ttatagatga acgggctttt ggaagttgga aattacaatt gaaacaagag
    18961 aaagttctta caaatggaga ttttgagctt ccagaagctc tttcctttgt tacctctgaa
    19021 aatacaacgg ttatagggac atccgctgtt acgattttcg aaagtgatca aaagggtgaa
    19081 agcgatttgt cctctctttt agattctaca gctcatcgtg ggatcagaat agatattcct
    19141 gtagaaaacc aaagaatcgg aacctttgag gggaaactat cttggatact tgcagatgtc
    19201 ccaggaaatt aacaggaaga gaagaatata acaaaattat tgagtttcaa gaagggcaca
    19261 ggtaccatag gctcttgtgt cccacagaga caagatattg ttatagtctt ctcttacata
    19321 tgattaaagg agcgattttc ttgagaccaa aacaacaatt atttataaca ctcctagtta
    19381 ttttaggggc actttttaac aatatatcag tgtcagctag tgcatttaat ttttcagtga
    19441 cgccgataac ttcagagaat caaatcgaca agagaaaaac ttacttcgat cttcaacttg
    19501 atccggatca ggaagtagaa gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga
    19561 aaatcgatat ttctgtgaat agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa
    19621 aaaacgagat tgaaaaagac atgagcctga tctttgatct ggtagattat gtgagctatc
    19681 cacagatagt gacgctaaaa cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc
    19741 cgaatgagcg atttgatggt gtattggctg gtgggatcac attccaagaa atccagacgg
    19801 aaaaggacca aacagaaact aaggatcaaa gcttatcgat tgagaatgaa tatacctata
    19861 ttattgcttt gttgatgcga cagacactga acgaagttgc tccgaacctt actcttcatg
    19921 aagttaaacc agatcaaatc aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc
    19981 aaaaaactta catcaatcaa gtaattattg aaacaaaaat tacaaaaaaa gggcattcta
    20041 aagttttata tcgagaagaa aaagaagcgt tgcagattgc tccgaataca aatttttctt
    20101 ttccaactgc tttaaatggt caaccgctaa ccccaggtga atatcacctt acaatgactg
    20161 ttttaggaaa tgaaaatgaa aatggaaaat tttcgagaaa aaaaggaaat actacgatca
    20221 actataccaa tcaatgggta tttgaaaaag attttacaat agacggaaaa gtagccaagg
    20281 aattgaacac gacggatgtt acgattaaaa aagacaataa ttggatttat atcttagtag
    20341 cttcactttt cttattgttt attttattat gcatactttt gtttgttttg agaaaaaaag
    20401 aagagaaaga aaagtaaggt taagataaca aatagtaaat ttttattatt gtaggatggt
    20461 tgggctcttt ttataataat ataaaatata ccattttatt aataaaaatg catactagta
    20521 aatgatataa ttcttataac aggtttgatc gcgagaaagg ccaaataaaa cgctctccaa
    20581 aatgttaaag aaagacagga cgatgtatat tatacaaaca agaatcgctg acaacagtga
    20641 ctcggatcca ggaaatcagt tttatcccca gctagttggt gaatgcatgg aaagtaatat
    20701 acgtagaaag agctgatgac taatgaatta tgagtttttt ttaagcaaag aagatcacag
    20761 aagttattca ttacttaaat atttagaagc aagctcaaat ttatcagagt caatatcgaa
    20821 tgttcaagaa gagttgtcgc tatcgacatt tctcttgaaa aaaacgatcg ataagctgaa
    20881 acatgattta caaaagttcg atctagagaa aaatttgcga gtaaccgtca gtgaattgga
    20941 tatatgtctg gaaattaatg gaaaatgttc tagcaaagct ctgttatctc gttatataac
    21001 ggattcttta tctatgaaaa tggtattggc tttctttcag gaaaaatata tctcagtaga
    21061 aaattttgct aaacagcatc acgtgagtta ttctgtggct tataaagtat tacaaagact
    21121 aaaaagaaat ctaaaaaaat atcaaatttt tttgaaaaga gaaagttgac agggaatcct
    21181 aaaaacattc ggcttttttt atataatctt tttaactcaa cagagataag ttttgtggaa
    21241 ttgtatacgc tacctataat tataaaaaca gaaaaaatgt taaaagattt aaaacagtat
    21301 tatcctctgt ctgcttatga aaaaaagaaa ttattccact ttttagcaat caacctgttg
    21361 gctgaacaag ctgattcttt agatattgct agcaatcagt cgctattttt ttcggatata
    21421 ggattttacc acttaaaagg agtattagga gaaaaatccg ttatttttat caataagaca
    21481 ctctcttggc tttatcttca tgataaattg gatcaaaaat acttgagaaa aaatgaagat
    21541 gaaacaatcg aatggttgaa tgaacgattc attgaaagtt ttgaaaatca gtttaatctg
    21601 ttgcgagaag agacgagcca attgatccga agagagttaa ccaaaataca ttttaaactt
    21661 ttgcattaca ctattaataa agctgatgaa tttccacttg atgtttcttt ttttagagaa
    21721 aactatccag cattttattg ctatttgata gagtatataa atgatctaac gtcaaaacgc
    21781 aaagagttgc ttcaagataa attttttctt tttttcagtt atttattatt attgatcaat
    21841 cacataccca ttcaactgat ttctgaacca gtaaagatca tcatcgattt ttcttatggt
    21901 gctgcctata atcaatttat caagaaaaat ttaagcttgt acattaatct gaacacggaa
    21961 gtgatagagg caggaaagcc tgagttcccg gatgttataa tcacgaatag gaataatcta
    22021 tatgaagaaa gtgcatctca agtagtggtt tggctcgatc cgccaagagc agtagactgg
    22081 gggaatctta caaagatttt gttgactatc caagaagaga aatacaaaga cagcaaaatt
    22141 gacaagcaaa tagacgattt tcctgaagaa atcatcgaca ggatcgaata gtgaacttct
    22201 tttagaaagt ccgactgctt tttctaaaaa taaaaaaact tggtacgcaa tcgctgtacc
    22261 aagttttttt atttagcttt tgattaagcc ataaataata atttatataa gaatactgct
    22321 acgacaccag cacagatagg tgctacaact ggtacccatg catatcccca ttgagaagaa
    22381 cctttatgtt tcaatggaag aagtgcgtgt acaagacgtg gagcaaagtc gcgggctggg
    22441 tttagtccag gacctgtagg accaccaagt gaagcaacta atgtccatac aaggaaacca
    22501 agtgctaagt gagctgtacc aagattatct tggaagaatg gtgcttttgt aatacctagg
    22561 gcaccaaaga ataaaacgaa tgaaccaaag aattcattga cgaaaccatt gaatttagaa
    22621 ccaacgctgt tgattgttga gaatgtacct aacacatggt tcacgtcttc tgtttggtcg
    22681 tagtaaggtt tgtaacaagc tactacgatc aattgtccaa tcgctgcacc aatcaattgt
    22741 gcaatgatat aaggaacaac ttcagcccaa gggaatagac cattagatgc taaagcaatt
    22801 gtaaatgctg ggttgatgtg gttaccgctg attccaccaa aaatcattgc tggcatcata
    22861 accccgaatc catacccgac agcaatcagc atccagtcac tgccatatcc ttttgtacct
    22921 tttaaatcta cgtttgctac agcaccgtta cctagaacga ttagtaatgc tgtaccaatc
    22981 atttccgccg caagacgggt cattagtgag taatccataa atacctccaa aagttataaa
    23041 aatgcaaaaa aaaagaaaca tcaacacgaa aagcatcggt gcagtgtttc gtgtcggaag
    23101 gatttctgca gtcttaacaa atgtgaataa aaaaatgaat tccattactt ataagaaacc
    23161 agaccaacag taaaactcca actcttttta aaagttctcc acaaatcacc aagcttaact
    23221 ataaatatac caaaaaaaaa acgttttttt tctcaaaaat tgtatcttag aaacagcatg
    23281 ttttgaaact ttcagaaaag gtttgttagt aaaatgaaag ctatacgtaa ggagtgttgt
    23341 ttgatgaaat tttttcaaga gtttttcatt attattttct tttctgtatt aggagaaggc
    23401 atacggattt ttgctcattt acctattcct ggaagtatct taggtattct ttttttattt
    23461 ttagcatttg aaatgcatct tgttgatcct gaaaaaatcg gagcaactgg tgatttttta
    23521 ttgaataatc tagctatttt atttgttcct gctggtgtag gtttattgga atattttgat
    23581 gatattgctt cgatatggcc tgttttactg ggagcagtcg tcgtgtgttc catcgtcacg
    23641 atggttgcag cgggaaaaac ggcagaaggt gtagaagctt tgctgggcta tgtccgtaag
    23701 aaaaaagcag gaagggtatc tgtaaacgaa atggaggaaa atcaaattga ctgagttttt
    23761 tgtgaatcca ctatttggct taagtttgac tttaggagtg tatgtattat ctgataaatt
    23821 tttgcggaaa tataaactgc ctgttttgaa tcctctcgta ttatcgattg ttgttattat
    23881 cttatttttg catttttctg ggatcagcta taaagactat aatatcgggg gcaatttttt
    23941 gactatgctg attactcctg cgacagtggc acttgccatc cctctttata aaaacttccg
    24001 tttactgaaa gagaattttt atcctgtaat ggctgcgatt ttagcaggaa tagtcgcaaa
    24061 ttgcttggtt agtgtagggt tcggttactt cttctctctt cataaagaga tggtgatctc
    24121 attactacct aaatctgtaa cgacggctat ttctgtcgat ttatctcata cgatgggcgg
    24181 ggtgaatgcc gtgacacttg ctattgttgt ctctacaggt atttttggtt cgctgatcgc
    24241 cactcatatt ttccgcctat tcaagatcga aagtccagtt gctcgcggtg tggcgttagg
    24301 aagtacttct catgctattg gtacagcaaa agcaatcgag attggcgaaa tagaaggaat
    24361 catttccgga cttgcgatat gcgtcaacgg aatattgacc gttttattgc taccgttatt
    24421 ctttcagcct tttgctggct tgttttgatg cgaaaaccac tattttatgc tacaataacc
    24481 aaaacgttcg agcataaaaa aggagggaaa gacgtgtatt tgtcttccga gtatcttaaa
    24541 caatttgata aagatctata ttataagatt ctcctcctag aaagttttga ggatcaagct
    24601 tggcataccg ctgcacaatt agcgcaagtt gttcagctgg atgcccgcag cgtgtcgaaa
    24661 tatctgaacg agctgtctaa aaactatcag cagtttagcg gtaagacaca ccctttgttt
    24721 acaaaaaatc accgttctgg ttataacttt tatgatacgc tcgattcaat tgaacatgag
    24781 cgttttttga tctatctcgt gcaaagtacg ctgaaattcc agttgctgca cgacattttt
    24841 tttgaagaat tccatacaat gtaccaattt gcgcaaaagc attacattag tgaatctact
    24901 gctcatcgaa aaatcaatga atggaaacag caactgcaaa cttacggaat tcggctgcaa
    24961 cgaggtacat atatcgctca aggcgaagaa gaaatcattc gactttactt gcatatgaca
    25021 ttctggcagt tgtttcgagg gaaaatctgg ccatttgaaa cgatttctca aatggacgta
    25081 aagaatatgg cagaacatat catggctttt tttaatgttc gtctgaacga gatcaaaaaa
    25141 agacgattgg aatacatgct aggtgccttt tttttaagaa aatcacagaa gcattatgtt
    25201 gtattgaatg agaaaaaaag acggctgatt tctgacaatc tgttgttcca gcgattttgc
    25261 caagtcatgg agccggtatt tcctaattat tttcaagtag aagacgagtt aggcgcatta
    25321 tttcttgtac tgatgactcg tgaagaatat tatagtgatc ctaagatacg gaagaaaatt
    25381 tttgattttc atcaacaagc aaaaacaccg ccctttacag ccctttcaga agcaaaagcc
    25441 gctctttctc tttatcagga agagcagggg ctgccagcag aaaatctaac cttcgaggca
    25501 gaaaactatt tattcagcag tcattttttc gcctatcttt ttcctaatgc gaaagaaacg
    25561 attgatggca atagtagtga ttttattaat catttagtta ttgaaaacaa ggaattgaaa
    25621 caatggctcg tacatttttt tgaaagtaga cacaaacatc ccaatcatct ggcatttaaa
    25681 aatcatacgt ttttgatggg tcggtatctg actgtgttta aaacattagg agcctttact
    25741 ccacagcttc caaagatcac gattctttta atgacagatt tcccattatt tgaagagcaa
    25801 ctgttagagg agggattacg gacatttttc cgtaatgagt atcagctgat ttttctgcca
    25861 acagattatc gggggagaga agttgatctt ctgatttcga caagtaaagt ccaccgcaaa
    25921 ccatgggcgg atcttgacta ttttattgtg accgaagaac taaaattaat tgattacatc
    25981 caactatctc aaaaatttga aatgattcaa aaaaaaacta ttaaaacaat aacaaagaag
    26041 gagaaaaaag atgacatttg aagaaatttt accagaattg aaaaaaggtg caaagattat
    26101 tcgtaaagga tggagtggat ttgaacttta tgttgttctc gtatccgatg atgaatgtga
    26161 tggctgtcca gtgacacctt acttattgat caaaacaagt gacgaaggtt tttccagctt
    26221 cgctcctaca gtttgtgata ttttagcaga tgattgggcg atcgtagaat gaaaaaccag
    26281 tatccagatc tagctaaaca ggttgtattt atcacaggag ctgcttctgg aattggtgct
    26341 gcgcaagccc gagcgtttct tgaacaagat gcttttgtat ttggattaga tcaaaagttc
    26401 ggtaaaatgg aagaactctc tttgcgattt cctgaaattt ttgcctacgc actaggtgat
    26461 gttcgacaaa tgaaagaact ccaacaagcg gtatcgaaat gccaggatcg ttttggtgaa
    26521 gttacgattt tactgaatac agcggggata ttggatgcct acaaaccatt gatggaaact
    26581 gacgaatcgc tgtgggatct gatttatgag acgaatgtga aaagcatgta tcagctgaca
    26641 aaaatcattc ttcctgatat gatccgccaa aaaagcggaa cgatcatcaa tatggcttcg
    26701 attgctggct tgattgctgg tggaggtggg atcgcttata caagcgcgaa acatgcgatt
    26761 gtcggattta ccaaacaact cgctctggat tacgcaggac agggtatccg agtaaaagga
    26821 attgctccag gagccattca aacaccaatg aatgcggctg attttgaagg gaaaggtgag
    26881 atggcagagt gggtggcaaa tgaaacacca gtcaagcgat gggcacagcc ggaagaagta
    26941 gctgagctga cgttatttct tgcctctcct caagcaagct acatacaagg aacgatcatc
    27001 ccaatcgacg gcggatggtt gttaaaataa ccttgtgttt ttttcaagct tctgtatgat
    27061 aaaactaaag agagaaacaa cggttgatga taaaaaaagt cgatgactgt tcccgacatt
    27121 cgatgccgaa cagccagttc aaaaaattct ttttaataat cgttaatatt cgaaactaag
    27181 cggcttttca cgaccttttg atataaacgg tgttcaaaag cttgcaactg cggtaacaag
    27241 tccag
//
LOCUS       DACERL010000033        27126 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.034, whole genome shotgun sequence.
ACCESSION   DACERL010000033 DACERL010000000
VERSION     DACERL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27126
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.034"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            32..104
                     /locus_tag="IXF54_001456"
     tRNA            32..104
                     /locus_tag="IXF54_001456"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Asp,seq:gtc)
     gene            108..180
                     /locus_tag="IXF54_001457"
     tRNA            108..180
                     /locus_tag="IXF54_001457"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Phe,seq:gaa)
     gene            185..255
                     /locus_tag="IXF54_001458"
     tRNA            185..255
                     /locus_tag="IXF54_001458"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:217..219,aa:Gly,seq:tcc)
     gene            268..341
                     /locus_tag="IXF54_001459"
     tRNA            268..341
                     /locus_tag="IXF54_001459"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ile,seq:gat)
     gene            353..424
                     /locus_tag="IXF54_001460"
     tRNA            353..424
                     /locus_tag="IXF54_001460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:Glu,seq:ttc)
     gene            690..1883
                     /locus_tag="IXF54_001461"
     CDS             690..1883
                     /locus_tag="IXF54_001461"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1404728.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2013..3485
                     /locus_tag="IXF54_001462"
     CDS             2013..3485
                     /locus_tag="IXF54_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1404729.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3568..4356
                     /locus_tag="IXF54_001463"
     CDS             3568..4356
                     /locus_tag="IXF54_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1404730.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4423..5091)
                     /locus_tag="IXF54_001464"
     CDS             complement(4423..5091)
                     /locus_tag="IXF54_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1404731.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5206..6918
                     /locus_tag="IXF54_001465"
     CDS             5206..6918
                     /locus_tag="IXF54_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404732.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6921..8690
                     /locus_tag="IXF54_001466"
     CDS             6921..8690
                     /locus_tag="IXF54_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404733.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8895..9461)
                     /locus_tag="IXF54_001467"
     CDS             complement(8895..9461)
                     /locus_tag="IXF54_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1404734.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9461..10417)
                     /locus_tag="IXF54_001468"
     CDS             complement(9461..10417)
                     /locus_tag="IXF54_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1404735.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10417..11073)
                     /locus_tag="IXF54_001469"
     CDS             complement(10417..11073)
                     /locus_tag="IXF54_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1404736.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11443..13461
                     /locus_tag="IXF54_001470"
     CDS             11443..13461
                     /locus_tag="IXF54_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1404737.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13888..16302
                     /locus_tag="IXF54_001471"
     CDS             13888..16302
                     /locus_tag="IXF54_001471"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1404738.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16650..18203
                     /locus_tag="IXF54_001472"
     CDS             16650..18203
                     /locus_tag="IXF54_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1404739.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18584..20368)
                     /locus_tag="IXF54_001473"
     CDS             complement(18584..20368)
                     /locus_tag="IXF54_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404740.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20383..22104)
                     /locus_tag="IXF54_001474"
     CDS             complement(20383..22104)
                     /locus_tag="IXF54_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404741.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22204..22965
                     /locus_tag="IXF54_001475"
     CDS             22204..22965
                     /locus_tag="IXF54_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1404742.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23932..24200
                     /locus_tag="IXF54_001476"
                     /pseudo
     CDS             23932..24200
                     /locus_tag="IXF54_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24872..>27126
                     /locus_tag="IXF54_001477"
     CDS             24872..>27126
                     /locus_tag="IXF54_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404743.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDG"
BASE COUNT         8672 a         4777 c         5662 g         8015 t
ORIGIN      
        1 cctgctcccg caattaaaaa ctaaaaacat tggttccgtg gtgtaggggt taacatgcct
       61 gcctgtcacg caggagatcg cgggttcgat tcccgtcgga accgtttggc tcggtagctc
      121 agttggtaga gcaatggatt gaagctccat gtgtcggcag ttcgattctg tcccgcgcca
      181 ttatgcgggt gtagtttagt ggtaaaacca cagccttcca agctgttgtc gcgagttcga
      241 ttctcgtcac ccgcttttag tttttatggg cctatagctc agctggttag agcgcacgcc
      301 tgataagcgt gaggtcgatg gttcgagtcc atttaggccc atttatttta ttggcccgtt
      361 ggtcaagcgg ttaagacacc gccctttcac ggcggtaaca cgggttcgaa tcccgtacgg
      421 gtcatggaag atactttttg agtatctttt tctttttatc tgaaagaatg attttaaaaa
      481 gcttagcaga gttatcttta tggatgattg tgctaagctt ttttggacag ttctaatgta
      541 aaatattttt atatttttta agcaaaaaaa tgaaaaaata cgagcagaaa actgttttat
      601 ctgctatact tggttcgttg caagttcccg aaaggatccg caggacggag atgccttgta
      661 accgaattta actaggggga atcattataa tggtagaaag acacttattt acatcagaat
      721 ctgtttctga aggacatcca gataaaattg ctgaccaaat cagtgatgca atcttggatg
      781 caattttaaa acaagatcca acagcacgag tagcgtgtga aacgtctgta acaactggtc
      841 ttgttttagt atttggagaa atatctacga cggcatatgt agatattcaa aaagtcgtac
      901 gggaaacaat aaaagaaatt ggttatacac gtgcaaaatt cggatttgat ggagatacag
      961 cagctgtatt ggttgcgatt gatgaacaat ctcctgatat tgctcaagga gttgatgaag
     1021 cacttgagat ccgggatgaa gataaaaaag acgtattaga tgagattggt gctggtgacc
     1081 aaggtttaat gtttgggttt gctgtagatg aaacaccaga attgatgcct ttaccaatcg
     1141 ctttgagtca tcgtctggta cgacgcttgg cagatttgcg taaatcaaat gaattaacgt
     1201 atttacgtcc agatgcaaaa tctcaagtaa cggttgaata tgatgatcaa ggacaaccgg
     1261 aacgcgtaga tacaatcgtt atttcaacac agcatgatga tgcagtagat aatgaaacaa
     1321 ttcgtcatga tgtcatcgaa aaagtagtga aggaagttat tccagctgaa ttattagatg
     1381 atcaaacgaa atattatatc aatccgactg gccgatttgt cattggtggt cctcaagggg
     1441 atgccggatt aacaggaaga aaaatcattg ttgatacgta tggcggttat gctcgtcatg
     1501 gtggcggtgc tttttctggt aaagatgcga caaaagttga ccgttctgcc agctatgctg
     1561 cacgttatat tgctaaaaac attgttgcag cagggcttgc tcgcaaagca gaagtacagc
     1621 tagcttatgc gattggtgtt gctcaacctg tttcgatctc aatcaatact ttcggtactg
     1681 gaacggttcc ggaagaagaa ctgattgctg cagtgagaga aaacttcgat cttcggccag
     1741 caggaatcat cgagatgctt gatttgcgtc gtccgattta caaacaaaca gctgcttacg
     1801 gtcattttgg tcgtacagat gtagatttgc cttgggaaca tacggacaaa gtagatgcgt
     1861 tgaaagcaag tttagcaaaa taagttatat tcagctggaa cgatcgccaa gtggtggttg
     1921 ttccagcttt ttttatggca gttaacctct tgaaaaagat agtaattatt tatctatttt
     1981 tagatcctgt cataatgaaa gggaggaata agatgaaaaa agaaacaaat gttactttgg
     2041 tcacagtgag tattttcatt gcaacgttca tgacggctat tgaaggtaca atcgtaacaa
     2101 ccgctatgcc aacgattgtc ggttcattac atggaatgga aatcatgaac tgggtatttt
     2161 cgatttattt gctgacaaac gctatgctta ctccgattta cggaaaactt gcggataaag
     2221 tgggacggaa accaatcttt atttttggga cattattgtt tattatcggt tcgttatttt
     2281 gcggtttatc tcaaagcatg attgggttga ttgctgctcg tgcgattcaa gggatgggtg
     2341 ctggggcaat gatgccagta gctttgacga tcatcggcga tctttattct attgaaaagc
     2401 gagcaaaagt tttaggtttg aatagttcag cctgggggat tgccagtgtt tttggaccgc
     2461 ttgcgggagg attcatcgtt gaaacgatca gctggcactg gatctttttt atcaatgtgc
     2521 cgatcggaat tctattaatt attttgattt ggctatattt aaatgaacca aaaatcatac
     2581 atgagtctaa accaatggat atttttggta gttttagttt gatggccatg cttgtctcat
     2641 tgctgttagg gttccaatta ttaggagacg aaggaatgag tccgcttgtt tctgctttgc
     2701 tgctaggtag tatcgtaatg ttaggtattt ttgtttttgc cgaaaaaaga gctaaagatc
     2761 ctgtcatcat gatgcattta ttcaaaaatc cactatttgt tgtggtcaac ttggctgcag
     2821 cattggctag tggttttttg atgggaatcg atgtttatat ccctatgtgg atgcaaggtg
     2881 tattagggct gaatgcagca attggtgggc tggtattagc accattatca ctagtctgga
     2941 tggtcggttc tttccttgca agcaagtgga tgactcaaat gaaaactcat tgggtcttga
     3001 gaattggact actaatcgca gctattgggg gcgcttggtt gtatcttatg cctttcagaa
     3061 gtaactattt atggtttttt gctatatcaa cggtcatcgg tataggctta ggtgtgacca
     3121 tgactacctc gactgtcagt gcacaaaata gtgtatctcg tgaccagctt ggtgtagcaa
     3181 cttcttttaa tacgttagtt cgaacaattg gtcaaacagt catggtttct atttttggtt
     3241 tattgataaa tggggtaact aaacgagaac ttgcagcagc aaatttaaca aatgatgcag
     3301 atatcatgaa taaactggtg aatccacaaa cggctaaata tattgatgcg gatattttgt
     3361 taccattaag aaaaattctt tatgatgggt tacattccgt ttatctagtt ggcttgttac
     3421 tcgtgatcat tgctttggtc ttaacgcttt tcgttcatga aaaaagtggt aaaataaaac
     3481 agtagtttcg gttaagatat gtacgtagcc gaatcattca aatttaaagt tattcaaaaa
     3541 taacttctta ctaaggaagg cgaaaatatg gcatttattc aagcaaatat ttattcgaat
     3601 gtgttagaaa tggaagtcaa tctaaatgtg atactaccgc aaacaacaaa aaaggtgatt
     3661 ggcaccagtt cagagcaaga gacaacggat atacctgttt tatatttact tcatggaatg
     3721 ggtggcaatc atagtgtatg ggaaagacgt acgtcgattg aacgttatgt agcaaactac
     3781 ggattagctg tcatcatgcc atctactgat cttggctggt acacagatac agtttatgac
     3841 atgaagtatt ggacattcat tgcagaagag ctacctgaga tttgtcatga attattcccg
     3901 caattaacaa gaaaaagaga aaaaacatat gccgctggat tatcgatggg cggatatggc
     3961 gcgttaaaac taggccttgc aaaatcagaa aatttcgcag cagtaatctc tctttcaggc
     4021 gctgtttctg taggaaatcg aatggatgat cttttgatgg ttcgtaaagc atctttttgg
     4081 gaagggatct ttggaccatt ggatcaaatc gaaggttcaa aaaatgatcc gcaatactta
     4141 ttgaaagaac tagtaaaaag cggaaaacca gttccaagat tttatatggc ttgtggggaa
     4201 tctgattttc tttatcaggc aaatcaggaa atggtatctg ctatgcagga acaaggcttg
     4261 gcggtcacct ttgaacaagg acctggcgat cataactgga tcttctggga ccaatggatt
     4321 caacgtgcat tggcgtggct aatggaagaa aaataaaaaa agagtcttaa aaagatagct
     4381 cctatagaga tcacaaacat agtgatctct ataggagctt ttttactgct cgttttctct
     4441 tcttttattt tgttggcata agccttcaat ataggtttgt gcactcgtta gaaagagtgc
     4501 tttttcattc aattctgcat gggactgcat actttcttta aatccctcta attgattttc
     4561 aactacataa ttacgcataa atagaactgt ttctttcaaa aattcatttc cgtccataat
     4621 tttttgtcga agctgctgtt catgctgctg atccattaac tgtccaatca ataaattgtg
     4681 gagagaggca atggcctgat gactttgggt gactgaaaat ccttcacgaa caagtaattg
     4741 gatcatttgc tccaaatgtt ctaatcgact cggaaaagcg ggcacagtat tgatttctaa
     4801 ttctgcccca caagggaaac gtgtataaag gtcatagtaa ttttccatat actgcagcaa
     4861 ctgttcattc caaggcaaag ttggatctgg aagcgctaat tcttcttcca ttgtttcagc
     4921 cattatttgc aagagctgtt gtttgttttt gaaataccaa tataaagcag gtgcttgaac
     4981 atgaattctg tttgcgatgt ttcgcatgga aagctgcgaa agagcttgtg tttcagccag
     5041 gatatcaaag gcggcctgaa tgatgattgc ttgagaaagc tttatttcca ttttatcaac
     5101 ctcgtgtatt gcattatctt tttaaacatg gtaaattgtt agtcggtcta attttacagt
     5161 gttaaattag tcctgtcaat ttaaacaaag gggtgtaatc aacgaatgaa attaatctta
     5221 cgacatgcga aaaaatataa agtagctgtt tttatttcct tgctgtccgt agcagtcatg
     5281 gttgcagcag cattatggca acctaagtta ctacagcaag tattggaagc tattattaca
     5341 gaagataatg atgaaatgaa aacaattggt atttacctga tcagtctggc tttgctagga
     5401 ttagccgcag gtgtaacgaa cacgattttc tctgcaaaag tagctcaagg cgtaagtgca
     5461 gatatccgag aagaagcatt tcgaaagatc cagacatttt catttggaaa tatcgaacag
     5521 ttctctgctg gaaatcttgt tgttcgtcta acaaatgata tcacgcaaat ccaaaattta
     5581 gtgatgattt ctttgcaatc actttttcga attccattct tatttattgg tgccttcatt
     5641 ttagctatga tcacgatgcc gcaattatgg tggatcatcg tagcgcttat cgttacggta
     5701 tttctgatta cggcactttc atttacaagg atgggcaagc acttcatgat cattcaaaaa
     5761 ctgattgatc gtgtgaatag cattgcaaaa gaaaacttac tagggattcg tgtagtgaaa
     5821 tcttttgtac aagaagaaaa ccaattgaag tcattcagca aagtaagtga ggacttaaca
     5881 aaacacaaca tcatagtcgg tacattattt tctgtgatga tcccgtcctt catgctggct
     5941 gccaatttgg ctgtggttgg ggcgatcttt tttgtgagcg atcttgttaa agatgatcct
     6001 acgttaattg gtggaattgc ttcattcatg aactacttga tgcagattat gatggcaatc
     6061 attattggcg gaatgatgat gatgatgact tcaagagcag ccgtatcgct gaagcggatt
     6121 gcggaaattt tagatgctga accagatctt acttatttag acgttccaga acaagagttg
     6181 acgggttctg tcacttttga ccatgtttct tttcgttatc ctggtgagga tacagatacg
     6241 cttaaagata tcagcttttc tatcaaacct ggagaaatgg ttggtatcgt cggggcgact
     6301 ggtgccggga agtcaacact agcacaattg atccctcgtc tatttgatcc gacagaaggc
     6361 aaagttgaag taggtggtgt agatcttcgt caagtaaatg aaaagagttt aagacaaacg
     6421 gtttcctttg ttttgcaaaa agcaatttta ttctcaggaa cgattgctca aaacttgcgc
     6481 caagggaaaa agaatgcaac agaaaaagag atggaacatg cttcttctat tgctcaagca
     6541 aaagaattca tcgaaaagtt atctgatcgt tacgaagcac caatagaaga acgtagcagt
     6601 aacttttctg gtggtcaaaa gcaacgttta tccattactc gtggcgttat cggtaatcca
     6661 aagatcttga tcctagatga cagtacgagt gcactggatg ctcgctctga aaagctggtc
     6721 cgagaagcac tcgatcgaga tttgaaggga acaacgacga ttgtcatcgc acaaaaaatt
     6781 gcttctgttg tcaaagcaga ccgcatctta gtattggatg aaggcagatt agtcggcgaa
     6841 ggaacccatg aggaattggt agctggaaac cgtgtctatc aggaaatctt tgaaactcaa
     6901 aaaggaacgg aggagtaagt atgactgatc taataaaagc aagtaaattt ttctatcact
     6961 atttgaagcg gtacaaactc tcctttttct ttatctttat cacagtgatc attgctacct
     7021 atctacaagt aaaggcacct caatacatcg gggaagcttt tcaagaatta gctaattacg
     7081 taggaggctt gatgcaaggc gtagacgata aaagcaaatt tgtttctgta atatggaaac
     7141 tcttgctgtt ttatgtacta gctagtgcag ccaactttat ctatagtatt ttgttcacac
     7201 aagtagtagg gaagtcaacg aaccgaatgc gtatcggtct attcaacaaa ttggaaaaat
     7261 tgacgatccg tttctttgat tctcatcaag acggtgaaat tttaagtcgt tttaccagtg
     7321 acttagataa tattcaaaat agtttgaacc aagctttgct tcaagtcatc acgaatgctg
     7381 ttttacttgt cgggatcttg atcatgatgt tccgccaaaa tgtagagtta gcttgggcta
     7441 caattgcttc cactccagta gccgttttga tagctgtctt gattatccgc aaagcacgga
     7501 aatatgtgga tcttcagcaa gatgaagtgg ggaaactaaa tggttatatg gatgaaaaaa
     7561 tcagcggaca acgtgtcatc atcacaaatg gcttacaaga agaaacgatt gaaggattct
     7621 tagagcataa tcaaagtgtt cgcgaagcaa ccttcaaagg acaagcttat tctggtctac
     7681 tatttccgat gatgcaagga atgtcactgg tcaatacagc gatagttatc tttttcggcg
     7741 gctggttagc attaaatggt gatttggaac gttcggtggc attaggctta gttgtgacat
     7801 tcgtccagta ttcacaacaa tattaccaac cactgatgca gatttcatct ggatacagca
     7861 tgatccaatt agcgattaca ggggcaagac gattgaatga aatatttgat gaaaaagatg
     7921 aagtcaaacc agaaaactgt aaagcatttg atggtgttga aaaaggtctt gcgttgcagc
     7981 acgtagattt tggttattct ccagaaaaac tagtcttgaa agatgtctcg atcaatgtgg
     8041 acaaaggaga aatggttgct cttgttggtc caacaggatc ggggaaaacg acgatcatga
     8101 atctgctaaa ccgtttttac gatgtcaatg atggttcagt agtcatcgat ggaatagata
     8161 tccgagatta tgatcttgat gcattacgta gacatgtagg aatcgttttg caagaatctg
     8221 tattgttctc cggaacgatc aaagacaata tcacattcgg caaaccggac gcttcagatg
     8281 aagaagtcat caatgcagca aaacaagcga atattcatga cttcatcatg acgttagaaa
     8341 aaggttatga tacagagata tccgaagaaa ataacttatt cagtacaggt caaaagcagt
     8401 tgatcagtat tgctcgaacg atcatcacaa atccatcttt attgattttg gatgaagcaa
     8461 caagtaatgt agacacggta acagaagcaa ggatccaaaa agcaatggat gaagcaatca
     8521 aaggaagaac cagctttgtg attgctcacc ggctgaaaac aatcttgaat gcagatagaa
     8581 tcgttgtctt gcgtgatggg gaagtcattg aagaaggcag tcatcacgag ttattagcag
     8641 aaaatggatt ttatgcggaa ctttatcata atcaatttgt atttgaataa agaggttgtg
     8701 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
     8761 tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt
     8821 atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt
     8881 tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt
     8941 tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa
     9001 gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc
     9061 aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct
     9121 ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct
     9181 ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat
     9241 gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt
     9301 tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc
     9361 gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct
     9421 tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc
     9481 gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca
     9541 agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc
     9601 ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat
     9661 tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc
     9721 tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa
     9781 tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca
     9841 aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt
     9901 gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa
     9961 gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg
    10021 gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt
    10081 gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat
    10141 aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga
    10201 attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc
    10261 aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat
    10321 cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat
    10381 gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga
    10441 gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag
    10501 actgagtaag taaccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct
    10561 tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct
    10621 tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc
    10681 tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg
    10741 ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc
    10801 agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca
    10861 agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct
    10921 gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag
    10981 ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct
    11041 accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt
    11101 attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc
    11161 ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg
    11221 gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa
    11281 gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg gtaaattaca
    11341 atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt aagagaagtt
    11401 ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa cattgcaaga
    11461 aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg aggggcagca
    11521 atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca gccggttcct
    11581 gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc ggcatttatc
    11641 acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata tgcatcagtg
    11701 atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc tcaagcacct
    11761 tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta catggatacg
    11821 cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca atatccttct
    11881 tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt tggcaacggt
    11941 tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc gacagcttgt
    12001 ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa tatcatcgca
    12061 acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg tggagcgact
    12121 acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa ttctggtaat
    12181 acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga ttctgtttgg
    12241 ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa caacatccaa
    12301 aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga aaatggctct
    12361 tcgacgaata attccggtaa caataccaat tcttcaggaa atgctggtac atcaaacgga
    12421 ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg gaagatctca
    12481 aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa gaacaatttc
    12541 gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc tggagcaaat
    12601 actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag ttcttctaat
    12661 acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag cgtctcaaat
    12721 aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa taactttatc
    12781 tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac gtcaggtaat
    12841 acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag cagttcgtct
    12901 caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa taaaaacggc
    12961 atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat ttatccagga
    13021 cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc aactacttct
    13081 gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc cactggtaat
    13141 acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa acatcatatc
    13201 acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta tccaggacag
    13261 caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt tactggttcg
    13321 aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag tcatggaatt
    13381 acaatggctc aactaatcga atggaacaat attaagaaca actttattta tccaggtcaa
    13441 acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa ttccacaaaa
    13501 atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag tagtaaaaag
    13561 actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt tgaactcgcc
    13621 tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc gttacaacgt
    13681 tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt cctataaaaa
    13741 gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa acaaagcaaa
    13801 aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat agaaacaagt
    13861 gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat tgagaaaaaa
    13921 tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc tgaaaagcca
    13981 aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca tgtagggcat
    14041 cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca aggatacaac
    14101 gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata tgcgctagat
    14161 acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg acgccaaatc
    14221 aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga tccggagtat
    14281 tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc ttatgaagca
    14341 gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga agaagtgatc
    14401 gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg ccaatggatg
    14461 ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt tgattggcca
    14521 gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc aaatattgaa
    14581 tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc agatactcta
    14641 ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga aattacgacc
    14701 cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa atcagatcta
    14761 aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta tgcaatcaat
    14821 ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc ttcatacggc
    14881 acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt cgctcaaact
    14941 ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc tgcttacaca
    15001 gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca agaagcaatc
    15061 gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt cagctatcgc
    15121 ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc aatcatccat
    15181 tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg gttgcctaag
    15241 acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat cgaggagtgg
    15301 gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa tacaatgcca
    15361 caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa caaaaaagaa
    15421 ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat tggtggagca
    15481 gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt atacgatatc
    15541 ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat gatcttaggc
    15601 gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga tgatgtggtc
    15661 gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc attagatgct
    15721 tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga tcgtgtatgg
    15781 cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt gaatgatggc
    15841 aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta tgaaaatctt
    15901 catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta taaagtggac
    15961 gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat tgctcctcac
    16021 atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta cgcaccatgg
    16081 ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt ccaagtaaac
    16141 ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga gctagaaaaa
    16201 gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt cagaaaagtt
    16261 attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct ttgtcaataa
    16321 ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc tgtctcagat
    16381 tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt tcatgttctc
    16441 aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg taatcatcaa
    16501 atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat tgagagtaca
    16561 taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa
    16621 tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat
    16681 tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca
    16741 agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat
    16801 atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt
    16861 atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac
    16921 ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag
    16981 cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga
    17041 agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat
    17101 cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat
    17161 tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg
    17221 gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg
    17281 gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt
    17341 ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt
    17401 tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg
    17461 atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc
    17521 cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg
    17581 aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt
    17641 cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt
    17701 ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg
    17761 atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca
    17821 ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag
    17881 tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt
    17941 tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact
    18001 atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga
    18061 atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt
    18121 ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat
    18181 cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt
    18241 cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt
    18301 cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga
    18361 tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt
    18421 gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat
    18481 tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca
    18541 tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt
    18601 gactattata aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt
    18661 tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca
    18721 aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa
    18781 tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag
    18841 gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc
    18901 cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac
    18961 attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg
    19021 cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag
    19081 aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc
    19141 ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag
    19201 ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca
    19261 atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt
    19321 gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa
    19381 atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag
    19441 aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca
    19501 caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat
    19561 taatgaaacg aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt
    19621 tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg
    19681 ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa acacccagtt
    19741 catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg
    19801 aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca
    19861 tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata
    19921 acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat
    19981 gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc
    20041 tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc
    20101 tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat
    20161 cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc
    20221 cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa
    20281 acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac
    20341 gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt
    20401 tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac
    20461 gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg
    20521 agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc
    20581 atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt
    20641 gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc
    20701 ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc
    20761 gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt
    20821 gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg
    20881 gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt
    20941 agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc
    21001 aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc
    21061 agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa
    21121 ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat
    21181 ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc
    21241 gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat
    21301 ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat
    21361 tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat
    21421 ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc
    21481 aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc
    21541 catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat
    21601 ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa
    21661 catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa
    21721 aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga
    21781 actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg
    21841 cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc
    21901 gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc
    21961 ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt
    22021 accggcgatt tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg
    22081 taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact tttcgattct
    22141 ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa
    22201 aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca
    22261 acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat
    22321 tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa
    22381 ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat
    22441 gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa
    22501 caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa
    22561 gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt
    22621 gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct
    22681 tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact
    22741 caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc
    22801 gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa
    22861 ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat
    22921 atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa
    22981 gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg
    23041 gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata
    23101 tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt
    23161 aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac
    23221 taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag aatgataaat
    23281 taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt attacaatat
    23341 gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat caaatagtcc
    23401 ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt gcttataaaa
    23461 taattataag tatttagaga tttttcttct aacattcttc aaacaagaaa aatcacaaag
    23521 gttattgtta tttttatata ttctttttaa gaatgattgt ttttttatag aaattttaaa
    23581 aaataaaaca cgttattgat ggatagttag tttgttattt ggtaatatta tagaagttga
    23641 ctggtagtga tttatttttc gtcgttttat aaaaatgtta ttttttggaa aaattcacaa
    23701 atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa agcagtatga ctaatataaa
    23761 tactattgtc atttatgcaa ttttttatgg atagtaattt tagaggggaa tcaatgaagg
    23821 aagaatagaa gggaaaaata gttgcaaata cgccagttag caaaaatagc aatacatctt
    23881 caagctttaa aattcgagag aatcatcttg gtagtggaat caactacaac aaaaggatta
    23941 aaccaattaa tagatagaat taccaatgga aaaatagaaa aggtagttat tctatacaaa
    24001 agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc tttgcaatat atggtacaga
    24061 atatagaaat tattgacatt acagaaaatg atgaagattt aatacagatt gtcactattt
    24121 gtagatacct tttacaagga aaccgagcca ataaagctaa gaagatgatt aaggagttga
    24181 tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa ataaagagca aaaagagtaa
    24241 ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt ggacagttta ttagtaatga
    24301 cggtttgaga acatccatga cgagaatgaa ggaacatcct aaatgcgttt agttgaacca
    24361 agtgccaaat aatatggcca aataagcgat cagttatcga tgaatcccaa gtattctaaa
    24421 ctatatattc attttgatgc taagcattga tatatttttt tgggattgat gaaggttaac
    24481 caatacaggg actcactgat gaaattctag agtctgacct tggtatcaac gatttagtga
    24541 ttgtttctaa tggaaaagta taaaagtata taagtacatt aacaaaataa ctattagggt
    24601 tacgctccag cttgtgcaag gcactggcag gaggaatcgt gaaatcataa ttatggttaa
    24661 gccacacgga caactaaaag tcatacaaag ctatcgaatc aatcaacaga agacaatcgg
    24721 caatcattag ctttgtttac agcttacatt gcaacgcttg agttgaaccg aaaatacgag
    24781 ggaggtaacg cagccatttt gagttgggga atctttttag aaaattatcg ggtaccttgt
    24841 tgtttagtca aaattataag ggggagtaat agtgaggaac aaatttcata aactgttaag
    24901 tatgtttcta gttatgctta ttgtgatcaa tcaatttggg aacataacgg ttctagctga
    24961 gtctttagca gaacagccag agcgttcgct gacgatcgaa gttgatcaag aggaggctgc
    25021 tttgggtgag cagttccagt tgcgagtcag aggaacacca gacctgctaa atgcattggt
    25081 cattgataag agtgaggatt tgattgaagg ggaagtgaag caactcaatg attacgaacg
    25141 tctgattact tattcttccc agcattcagg taaatacgaa ttgaccgcaa gaagcggtga
    25201 tattcaatca aatacggtct caatagaaat cagtgtacca gaagaaagca caagtgagtc
    25261 ttttaatgta ccagaagaaa gcacaagtga atcttttaat ataccagaag aaagcttaag
    25321 tgaatctttt aatgaagcca gatcatctga gccatctata acgcaggaat ctcagattga
    25381 aagacaagag gagctcttga attatgacat cggcatctct gctgagcgcc ccactttgcc
    25441 gagcaggcat acacaaacgt taaagggata cattcaagga ggcgctggcg atattcttga
    25501 agaatatggg gagatggtcg tacgagttcc tagagcatcg attggcttaa ttgagttcat
    25561 tagagtggag gtcgctgatg attcaccttt aaagccactg gcattttacc aagaaggcac
    25621 agacgttgtt tttccattta cctttgatta tgaaggatta aatgaagccg ctcagtcgta
    25681 tacattcagt atcacgtttg aagttgcttt ctttcaatca ggcggtactg agccaaataa
    25741 tgactttcaa gtgagtctta cttatgctga gcgagcagca aacacggaat tttcaattac
    25801 gcctattcct tcatcaagac ccattttttc gaaattatac tatggctcta gagaaatcga
    25861 cagccaagga aatcttggga atctgctgat tgacgggcaa gacgtgacca acagtaataa
    25921 atttattttg caggtaaatt atgaaggatc acgaaaagac ggtgtagtgg ttaccgatgt
    25981 actaccgcaa ggaacaactt tggcaccgaa tccgactaca tggagttcca ctggaaatag
    26041 agaacctatt tcaggtgggg ttcggatcgc agaagtaacg aatccaggtg aagcaaacgt
    26101 tagaactcat tatgtttccg aggaatttgt ggacaaagta tcttttaata aggaaacgaa
    26161 tacacttgtt gtggactttg gtaatctagc agagggtcaa tcgtatttta ttgaatatgc
    26221 tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga caagaggatg ggttttctcg
    26281 gaatcgagcg agtatgacct acaataatca ggtaatgagc cgagactacc gcttgagaat
    26341 ccctgaaagt aatcttccgg caatctcttt aagaaaaagt gtggacaaaa atgttatcaa
    26401 tctcaatgaa aatgaactaa cgtatactct gcatgtctcg atgttttctg gaacaagaaa
    26461 tacgcttgtt attgaagatg ttttggaaga aggcatggaa tttgtgtcat ttctatcaga
    26521 tattccctct ttcattgatg ccgaagtcaa taatggaata gttcgtttca gtgcgaataa
    26581 tacgattggt ggtccgacaa gttttaccct ctcttttaag gtcaatgtag aggcgtatgc
    26641 ggtcggcgat caaattttaa actttgcaac gcttgtgaat agtgacaatc gattgaatac
    26701 gagtactgta agaacaagga aaatcgatgg gcgtattaaa gtaattaaaa ctgatgagaa
    26761 tggccaaagg ctggcaggag caaaatttga aattttatca agcgattcac aagtgataca
    26821 agaaggtata acggcagaaa acggagaatt tttaagtgaa ccgcttcctg taggtagcta
    26881 tcaagtccga gaaacagaag caccggaagg gtatctttta gatgcgacgg tacatgatgt
    26941 ttttataaat gaatactcca tagaagaagt gacgttaacc attgaaaaca tccttgatac
    27001 gggtggtgtt gaactaatta aacatgctga aggcagtggt gaagtccttc aaggagctgt
    27061 ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggtttaacta caggagaaga
    27121 tggaaa
//
LOCUS       DACERL010000034        26684 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.035, whole genome shotgun sequence.
ACCESSION   DACERL010000034 DACERL010000000
VERSION     DACERL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26684
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.035"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..203)
                     /locus_tag="IXF54_001478"
     CDS             complement(<1..203)
                     /locus_tag="IXF54_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1404744.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFT"
     gene            complement(225..1199)
                     /locus_tag="IXF54_001479"
     CDS             complement(225..1199)
                     /locus_tag="IXF54_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1404745.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1499..2371
                     /locus_tag="IXF54_001480"
     CDS             1499..2371
                     /locus_tag="IXF54_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1404746.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIIIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(2953..5151)
                     /locus_tag="IXF54_001481"
     CDS             complement(2953..5151)
                     /locus_tag="IXF54_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404747.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            5383..6375
                     /locus_tag="IXF54_001482"
     CDS             5383..6375
                     /locus_tag="IXF54_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1404748.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            6435..7856
                     /locus_tag="IXF54_001483"
     CDS             6435..7856
                     /locus_tag="IXF54_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1404749.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPDKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            7849..9348
                     /locus_tag="IXF54_001484"
     CDS             7849..9348
                     /locus_tag="IXF54_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1404750.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            9387..10781
                     /gene="uxaC"
                     /locus_tag="IXF54_001485"
     CDS             9387..10781
                     /gene="uxaC"
                     /locus_tag="IXF54_001485"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1404751.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            10940..11623
                     /locus_tag="IXF54_001486"
     CDS             10940..11623
                     /locus_tag="IXF54_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1404752.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            11625..12461
                     /locus_tag="IXF54_001487"
     CDS             11625..12461
                     /locus_tag="IXF54_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1404753.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            12472..14226
                     /locus_tag="IXF54_001488"
     CDS             12472..14226
                     /locus_tag="IXF54_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404754.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(14502..14843)
                     /locus_tag="IXF54_001489"
     CDS             complement(14502..14843)
                     /locus_tag="IXF54_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1404755.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(14911..17082)
                     /locus_tag="IXF54_001490"
     CDS             complement(14911..17082)
                     /locus_tag="IXF54_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1404756.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            17293..18150
                     /locus_tag="IXF54_001491"
     CDS             17293..18150
                     /locus_tag="IXF54_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1404757.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(18221..19078)
                     /locus_tag="IXF54_001492"
     CDS             complement(18221..19078)
                     /locus_tag="IXF54_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1404758.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(19063..21765)
                     /locus_tag="IXF54_001493"
     CDS             complement(19063..21765)
                     /locus_tag="IXF54_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1404759.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(21778..23067)
                     /locus_tag="IXF54_001494"
     CDS             complement(21778..23067)
                     /locus_tag="IXF54_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1404760.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            23217..24263
                     /locus_tag="IXF54_001495"
     CDS             23217..24263
                     /locus_tag="IXF54_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1404761.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24265..26418
                     /locus_tag="IXF54_001496"
     CDS             24265..26418
                     /locus_tag="IXF54_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1404762.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         7882 a         5382 c         4919 g         8501 t
ORIGIN      
        1 tatgtgaagt acgttttcgt caaaggaaac gtttcttttg agcgcagcca ttttctaatc
       61 actgcaacca tcgcatacga tgcaggaccg aaagtaaaaa ctcccaaacc tgcgatgact
      121 aaaccgcccc acagaaaatt caaataaacc aactgtaaaa tacgatttag ccttccgttt
      181 attttatcta atgactgcag catatctttc cctctctttc tatcttagta aacgccgtct
      241 tctcctagct ttttggcaat cttattagct aaaattacca taaacaatcc tacgacccct
      301 ttaaacaatc ccattgctgt actgaaactt aactgtccat tcttcaatcc agctgtataa
      361 atataggtgt cgaaaatttc tgcaacgctt cgattcaatg aattcaataa caagtacata
      421 tgctcgaacc ctaagtctaa tgtctgaccg atttttagaa tcagcaaggt aatgattact
      481 ggacgaattg ccggcaatgt aacatgccat gttttgcgta agcgtcctgc tccatccatt
      541 tctgcagctt catacaactg tgtatcgact gcagttatcg ctgaaagata gatgatcgta
      601 gaccagccca actctttcca gatcacttgt ccgatatata ctgtgcgtgt ccattcaggg
      661 gatgttagaa aactgatttt ttcaaatccc attgttgcaa ggaattcatt caatacccct
      721 ccgtcaacat tcaaaaatac ataagtaatc gaaacaatga ttacccaaga catgaagtga
      781 gggatgtaaa tgatcgtttg aatcccgttt ttcaatcgtt tgttcgttac ttcatttaat
      841 agtaaagaca aaatgattgg catcggaaaa aacacgaaga tatttaatcc gaacaaaatc
      901 aacgtatttc ttaataatgt aaagaatgtc ggttctgtaa acaaacgaat gaaatgtttg
      961 aaaccgaccc aaggacttcc cattattcct aaaaatggtt gataatcttg aaaagcaata
     1021 acgagtcccc ccataggaag atacctaaaa atcaagaaat aaaggactcc cggcagaatc
     1081 atcacatata aaaatttatt cgttttgatt cctgcaatta ttttttttcg tcttctgagc
     1141 ttttcttctt ttttcgatag cgtcagttga aatggttctt gtttctttac actttccata
     1201 agcccctcct tagttttgtg aatgtttgct gcttatgact tcatgataga taactatctg
     1261 aattacgtat ataatcattt ttatcgtatg taaaaacgct tacatagaaa tgatttttgt
     1321 gctatgcaaa acgataaagt gtattttttc acaaataaac tacgcatagc aaaccttcta
     1381 tttttttcta tctgacactt agcataggaa atgattatat acgtaatctt tcaccgatga
     1441 taaattgaac atatagaaac agaaacattt aatttatttt gaggaggagc ctatctatat
     1501 gaaaaagaaa gtctctatag gagaaagaag ttttacactt ttcaacaata cgtttctaat
     1561 tttgctagct ttgatctgta tcgttccttt tttaaatatc atcgctacat cttttgcttc
     1621 tacacaagaa gtcgtcgcga aaaagtttat cctttttcct actacctttt ctttagacgc
     1681 gtatcgctac attctttcta cgccgaccat ttttagagca cttgctgttt cgattggtgt
     1741 tacaggtgtc ggaacgatcg tcagtatgtg tgcaacttca ctgatggcct acggtctttc
     1801 cagaaagtat ttgtttggtc gaggtttcgt caactttctt gttgtatttt ctatgttgtt
     1861 cagcgggggg atgattccaa cgttcttagt tgttcgttct ctaggactgg tcaattctta
     1921 ctgggccatg atcctgcctg tggctgttaa tgcaatgaat atgatcatta tgagaaactt
     1981 tttccaagcc ttgccggata gtttggaaga atcagcaaaa atggatggtt gcacagattt
     2041 tggtgtattt ttcaaaatta tgctgccatt agcgttaccg tctattgcta ctatctcgct
     2101 tttttatgct gttacttact ggaacactta tatgactgct attttgtata tcaacgattc
     2161 atctaaatgg ccgatacaga ttcttttacg ccaaatcatc attgtatcaa gtgggatgca
     2221 agcagaaagt agtgccgttg atgtgattcc accagcacaa acagttaaaa tggctgttat
     2281 cgttattgca acagtgccta tgctgattgt ttatcctttt gttcagaaat attttgtcaa
     2341 aggagcactc gtaggttctg tcaaagggtg attatgtact gaacgcaaaa tggtagttgt
     2401 gtcagaagtg gtcagcttca agaaataaga aggcatttac gaaaattgct tcttaaattt
     2461 ttgtgaaatt tcggcttatt tttcgaagaa tctactcctg gaacaccgtg tatcagaggt
     2521 tgtgaaagag acgttcagcc ttgagcgatg ataaaaactc tcacaaacat tgcgacgagt
     2581 aacccacagg agcaataaga agcgattttg aaaaatagct tcccatattt cacaaaatta
     2641 tgccttattg tcgatagcct gtctcttgaa cgccatttat tcgtaaaaaa gaactgtgac
     2701 aagacttttg tcacagctct ttttgcgtgc ttagccttga aatttaacta tccataaata
     2761 caaatactag ggttattcta acttctttta ttgcttgaat cagtccacaa gaaaaacagc
     2821 ctcttgatta aacggcgttt atcttgttga ttctgcggca ataaaatcag ccagcagcaa
     2881 aacatgaagt gctgtttttt gctgtcctct tttattgctt gaatcagatc acaagaaaaa
     2941 cagcctcttg attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt
     3001 tttaaagaaa cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt
     3061 tacggagagg ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac
     3121 aaaatcacca aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt
     3181 ataatgcaac ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac
     3241 gatatgcata atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca
     3301 ttctgtgatt ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata
     3361 attgttcagt gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc
     3421 caataactgt cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact
     3481 caaaggattt ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac
     3541 ttcctgattt ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct
     3601 aataagtgtt ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg
     3661 accagtattt acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc
     3721 attcagcgtt tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt
     3781 tacacgtttt tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc
     3841 tttatccatt cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg
     3901 attttccttt ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg
     3961 ctccccaccg tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc
     4021 attttgagaa ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg
     4081 atttctaaat aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg
     4141 tgcacttaac gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag
     4201 ttctttccca ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg
     4261 acgggcaaat ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa
     4321 agacactgca actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc
     4381 aacttctaat ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc
     4441 tttttctctt atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg
     4501 ttgctcttta ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga
     4561 aagtatgtct tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc
     4621 aattcccaat gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc
     4681 tttgtttttc ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt
     4741 ttcaacttcc tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc
     4801 caaactgact aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc
     4861 tttcctgatt tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc
     4921 cgagaaattt ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca
     4981 ttcaacgtta gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc
     5041 gatcgattca taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc
     5101 taataaaaac atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct
     5161 ctcctccttt taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa
     5221 atgagcattc cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta
     5281 acgtgtgcaa tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat
     5341 gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat
     5401 atcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt
     5461 ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg
     5521 ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc
     5581 tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat
     5641 tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca
     5701 gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag
     5761 cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc
     5821 aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta
     5881 gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt
     5941 cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct
     6001 gtcaatccag cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg
     6061 gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata
     6121 gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct
     6181 ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta
     6241 cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa
     6301 ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg
     6361 atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgcgt gcataacaca
     6421 gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg
     6481 taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc
     6541 agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac
     6601 aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac
     6661 tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca
     6721 atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata
     6781 tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata
     6841 ctcccccaca gagcttcccg gacaaactga cagccttttt ataccgacgt ttcaaattaa
     6901 acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa
     6961 aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt
     7021 tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc
     7081 gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag
     7141 cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat
     7201 tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta
     7261 aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat
     7321 tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat
     7381 tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg
     7441 accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat
     7501 taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag
     7561 aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc
     7621 gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt
     7681 ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag
     7741 atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc
     7801 aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc
     7861 ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt
     7921 ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg
     7981 caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc
     8041 tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat
     8101 gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt
     8161 cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg
     8221 attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg
     8281 atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt
     8341 ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt
     8401 ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt
     8461 gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg
     8521 aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa
     8581 gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat
     8641 gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt
     8701 ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca
     8761 gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac
     8821 tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt
     8881 tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga agataagtct
     8941 ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa
     9001 aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc
     9061 tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg
     9121 tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa
     9181 ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta
     9241 gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa
     9301 aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac
     9361 agtataaaaa gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg
     9421 attgggcgaa aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact
     9481 gtcacttaga tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt
     9541 ggttaggagg cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa
     9601 aaatcacagg aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa
     9661 aaattatcgg aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta
     9721 ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt
     9781 tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt
     9841 gtacaacaga tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac
     9901 cagatttgaa ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag
     9961 gatttaatgc atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc
    10021 aagaactgat agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg
    10081 tttccgacca cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag
    10141 agatttttca aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc
    10201 gtacagaaac actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc
    10261 tgcacatcca tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata
    10321 ccggctatga tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaatc
    10381 gtgcagaaga aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact
    10441 atcctgcgat tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt
    10501 taggttctgg atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat
    10561 ttgctgatgg tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc
    10621 tgtcctacac tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg
    10681 tcatgcgagg agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta
    10741 gttatacaaa tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta
    10801 acgaatcaaa aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa
    10861 gacggaccaa atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct
    10921 ttatgaaaga agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga
    10981 aaaagatgaa aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc
    11041 acccgagatc cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca
    11101 ggaaggaagg ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca
    11161 atttggacat aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta
    11221 tttaaaaaat ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt
    11281 actgacatct gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact
    11341 tggagactat ccaaaagcaa tgcgtgcatt tgctgagaaa caccatattc ctcttttaga
    11401 tgtgttctca agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt
    11461 ctacctgcat cttatgcctg atacttgtaa aaattaccca gaaggactaa aagacaatac
    11521 ccactttagt ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga
    11581 atcccgtctg cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt
    11641 taggcatccg tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat
    11701 cacagcttca cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta
    11761 aattaactga catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca
    11821 atatcgaact ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc
    11881 gtgcacaagc attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca
    11941 agatgattgc tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg
    12001 aagaagctta tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt
    12061 ttggcgtaac tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag
    12121 ctaaacgttt aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga
    12181 atttgatgca taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca
    12241 atttacatgg ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga
    12301 tccgcatggt acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca
    12361 aatatgagcg tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg
    12421 aagcaataga acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa
    12481 cgaatacaga aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt
    12541 tatcagggaa attattggcg ttttgccatg tcaggactct tttttattat caaacatctt
    12601 ccatcttggt taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat
    12661 tcttctcagt tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa
    12721 aatatattga cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa
    12781 gctggactaa gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa
    12841 gaattgcaat ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg
    12901 ttgtcctctc aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta
    12961 ggtatcacgc tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc
    13021 gccgcattcg ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg
    13081 caagaaatgg aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct
    13141 cgagctcatg ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc
    13201 tatgaaaaag gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct
    13261 agttttcaga tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg
    13321 cgcatccaac ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc
    13381 gtttctggaa tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct
    13441 attggcgaac tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa
    13501 catttggatg gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa
    13561 ccgattatcg atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg
    13621 ccatctggct ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact
    13681 tcaggaaaaa tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga
    13741 aattatttag ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac
    13801 atcacttatg gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg
    13861 caactttctt cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat
    13921 ggcaacaagc tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt
    13981 cagccaaaaa tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa
    14041 aaaatccagc aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat
    14101 cggctttcaa cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaattgtt
    14161 gaaataggta cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat
    14221 gaatgacaaa aaataggagc tatgacaaga cttttgtcgt agctcctatt tctgaataaa
    14281 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcatcaaac atgagaagct
    14341 gttttttgaa attgttcctt gtaactcaga gttaaacggt aggctcacga cctcttggta
    14401 aatggtgttc aagagacagc ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc
    14461 gaacgtctct ttcacaccct ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg
    14521 cgaactggtg tcatgatgat acggttcaag tcattgatga ttgtactgaa gccttgctct
    14581 ttttgcatca agtcattgat tacttcttct gattggattt tcacagccat ttcttgtgct
    14641 ttcattgcat cttcgtcgct gaactcttgt ccactcattt gtttttcttg taattcgatt
    14701 tgcatcgctt ggaagtcttt gaacagtttg tgtgcttctt cgtttgcttg tactttctcg
    14761 tacgcttctt tcaacgcttt gaattctggt aattcacgga tctcacgttc gatttgattc
    14821 gcgctatcgt agatgtttga catgttatgc cctcttttct tttagtagtt actctctatt
    14881 gtaacatggg gttattcgtt ttcaaagggg ctactctcct tgatatttcc gccagaggtc
    14941 tttagccgct tcaccaattc cctgaacgcc ttctttcacc atatcgccga agtttttcag
    15001 cccttctttt gcttgttcac catattgatt caagttatct tgccaatttc cagactctcc
    15061 attagaattt cctgtgtttc caacttgatc tgcagctacc acttgtcctc ctgtggcata
    15121 ggcatctgct acactaaatc ctgtttttga agtatacgga aggatgcctt ctgccgctga
    15181 tttgaagact ttcccgacaa catttccact tgtcccttcc aaatagtgca gcttgctgga
    15241 ttcttcgtaa cccatccatg tcgaaatcac tacgtctggc gtgtaaccga tcatccattg
    15301 gtcatttgct tttgtggcat cgaaattagt ttctgtggtt cctgttttcc ctgctatcgt
    15361 ataacctgca ggattcgcag cagccgctgt tccatttgaa aatgtaccaa gaagcatgct
    15421 ggtcatttca tcggctgttt cctttgatat gacctgtttt ggttttttgt ttgtgttatc
    15481 gacgatcacc gctccagtcg aatcaacgat tttcgtgata agatgcggcg tatacatctg
    15541 accatcattg gcaaatgcac tgtaagcacc cgccatgatc aatggagata ctcctttttc
    15601 cagtccacca agggctaagc cataatactt atcagatttt gctaaaggca ggccgaattc
    15661 ttctgcttta tcatatcctt tttgtaaacc gatctcatgc aaaagccata cagcaggcaa
    15721 attcaaactt tgtgcaacag cttgatacat cggtacttct ccttggtatg tcccgtcaaa
    15781 attatgcgca tcgtaataac tttgcggttc gtctttcaag atactggaag gcttgtatcc
    15841 agcttccagt gctggtgtat atacgcttaa tggtttgatc gttgatcctg gtgaacgacg
    15901 catctgtgta gcaaagctga atcctctaaa gacatgatct cctcttcgtc ctactagtgc
    15961 ttgtacacca cctgtattcg gatctaaagc tactgaagca gattggacca tggcaccatc
    16021 tgaagcattt gctgggaata atgcactgtt ttgataagta gcttctaatt gcttttgata
    16081 atcctgatct aaagctgtat agattttata gcctttattc atgacatctt cttcatcaag
    16141 accatactcg ttaactgctt catcgatcac tgcatcaaaa taataaggat aacgataacc
    16201 agaatctgaa ttatcgtatg tgtcattaag atacgcgctg atatctgtct ggctttctgc
    16261 ttgttcttgt tcttttgtga tttttccgtt ttcttccatt acacttagta ctgtgttttt
    16321 ccgatcattc gcattttttg ggtgatcgat cggattataa ataccaggac ctttcagcat
    16381 tcctgccaaa gtagcagcct cagacagcgt aacattcgtt gcatctactc caaaatactt
    16441 tctggaagcg tcttggacac cccatacccc gttgccaaaa taggcattgt taagatacat
    16501 ggtcaagatt tcttctttgc tgtacttctt ttcgatctcg attgccaaaa ataattcctt
    16561 cgcttttcga tcaaacgttt ggtctaatgt cagatacgca tttttggcaa gctgctgagt
    16621 gatcgtactt cctccgcccc ctgatgttcc gccagacaag accattcgga ctgctgcacg
    16681 ggcaatccct ttgatatcaa acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat
    16741 tactgcatct tggatatagg gagaaatcgc gtttaattct acatatgttc ctttttgggc
    16801 gtacagtgtg ccagcttctt cgtttttctt atcataaatg actgtcgatt ggcttaatcc
    16861 tgattttaac gtttcgacat ttgcttgttt tgctaaaata aagaggtaga tacttgtcac
    16921 taagacacag atcaaaccaa tcagcaaaat caatttgttc acatgatatt ttttccagat
    16981 gcgctttcgc catcgatgga attgtccgag atatggtctg atccaacgcc aaaaactgat
    17041 caaagcagct tttatctttt ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct
    17101 atttgattca atcgtcttat tttaccatag tgaaagagtg ttttcttcct gcttaagttg
    17161 tagttcaata tgttttttta cgttagtgca agtagagtaa cataagaatg aacagaaaaa
    17221 tgtaagtttt ttcttaaata tgttttaaaa atggtacaat tagtctgcga ttgcactgaa
    17281 aggagtacac ttgtgaaaat cacgatcact ttacctatga accatcctga aacaacaatt
    17341 cgcgacttgc ttgaaaagga atggttagtt cctagaaagg ttcgacactt tttgcgtata
    17401 cgtaaaaacg tttggatcaa tcaacaacct gctttgttcc atcatgaagt aaagggcaat
    17461 gaccagatca cattgttatt cgaagaaaca gattataatt atcaagaagt tcaattagga
    17521 gatgcgaagc acatccgtgt tttatatgaa gatgagcatt tgattatagt ggataagcct
    17581 gcaggaatga agactcatcc aaatgaaccg acagaaaaca gtaccttact caatcattta
    17641 gctgcttatc tcgacactaa acagcaacga ccttacgttg tccaccgctt ggacaaagaa
    17701 acaagcggtg ccgttttatt tgcaaaaaat ccatttgtct tgccgatact tggaagaatg
    17761 ttggaacaaa aaatgatcta tcggagatat caagccatcg tatggggaga aattgccaaa
    17821 gatatgacca tcaccaataa aattggacga gatcgccatg atcggagaaa aagggtcgta
    17881 gatgataaaa atggtcagag cgccttgaca catgtcactg tcaacaaagt actttctgaa
    17941 caaacacaag tctattgtgt tttggaaacc ggacgtacac accagatcag agttcattta
    18001 gctcataagg gacatccgat cgttggagac cctctctacc aaaatagaac agctgaacga
    18061 ctgatgctcc atgcattaga actgcacatg atccatccat ttactcaaga aaaaatagtc
    18121 ggtaaagctt tacctggatt atgggaatag aaataaaaaa gaacagagtc ttatcagcag
    18181 ataatgataa gactctgttc tttttgtgta atttgtctgc ttacttttga tgttcgtgtt
    18241 ctgctaacag caaactaccg ataatcccac tttcattcgg cagtccccaa cgaacgatat
    18301 attcacttac ttgaggtgtt tccatataac cacccatcaa ttcaacaaat tgctgacgga
    18361 ttttttgaag gagatgatcc tgattcatta ccccaccgcc taaaataatc ttttcaggtg
    18421 ctaacgtcaa cgtatagttg atcagtgctt gagcaatata ataagcctga atagcccaaa
    18481 caggatgatc ttgcggcaga tcttgccctt tgatccctgt gcgtgcctca atagacggtc
    18541 ctgctgctaa tccttctaaa caatctttat gataaggaca agtaccttca taatggtcat
    18601 ctggatggcg tttcacccaa atatgtccca tttcaggatg tgcgatccct gaaaaaattt
    18661 cttcattgat cacgacacct gcaccgattc ctgttcccac ggtaaggtaa atacaactat
    18721 tttttccttg tgctgctcct tgcttcaatt ccccatatgc agcagcattg acatccgttg
    18781 tccaagcagc aggaatattg tagcgttcct tgattgtgcc aagaaaatcg aattgtgccc
    18841 accctttttt gggagttgcc aaaacgtatc cgtaattatc ttttgttttg tcgataccaa
    18901 tcgggccgaa tgagccaatc ccgattccct tcaattcata acgatcaaaa aattcaaata
    18961 cctgatccaa tgtttcttct ggtgttgtgg tagggatact taatttttct aaaatttctc
    19021 caccatctga aaccgcacac acaaattttg ttccgcctgc ttctattccg ccatacattc
    19081 gttttcctcc tcgaatccga ttgtccgaat ctcataaggt tggattattg ggacaaccgc
    19141 ttgcttcgtt ggttcttcaa gcagattcaa tatgacagtt tttccattgt ctttagttat
    19201 ttccaatttt tctaaatgac tagacatatt gaaacctctt actactactt tatcactaaa
    19261 cttgctgcgt ttcaaagctg taattgcaaa cgtttcactt tttatcgtta aatattgttg
    19321 ttttgaaatc aatgttcctt catgatgttt gatttgttga gtggaaaaag gaatctgtgc
    19381 tgcgtatgca tgcaaataag tagagaattt ttcttctggt ccatgtagtt caatgctgta
    19441 attgaaccga tgctcaccta gacactgtgc ttctggtgtt ggaaaatatc cccaatcgcc
    19501 tagttctccc acactgcgaa gtagcgtcac tgcgatttgt ccgtcttctg ttacttcata
    19561 ttcattcaaa ccaaagtttc cgactgttac accgtactct tcatcatgaa tattgacaaa
    19621 tgcttgctga tgttgcggat tcgtggggtt ttcccaactt ttagacacat ggtttgggcg
    19681 cttcaccact tcaaaaatac tatctgcttc gtgatgttcc acatgtaact ttgtaggaaa
    19741 caagacacgt aagcgatgat ctttcatctg attgtcgaga gtcgtttcaa agtcgatttt
    19801 tttactatct tttctgattg tcatgattgt ttttatttga aggattcgtt tttctttgga
    19861 tcgttctgct tttcgataac gaaattccac aaccatctgc tgttcttttt ctaaacgctc
    19921 gtctgcagaa acagggattt ccatttcctg tagaatactt atttttgcta tttccggtgt
    19981 atctactaat acttttactt cagagttttc tttgttactg gataggattg gctgatcaca
    20041 gaaaggttgt ttaaagatat actcatttcc tatatcgccg atatcttcaa aaatatgcag
    20101 attattcaac tttcgtttgt ttttgcgatc ttccattgta atggtaccat ttttttcaat
    20161 cgtcaaatgt agagggccgt tttcgatgat tcgtccactc tgatgaatca ttgtttcctt
    20221 ttcttctgct ttcgcttccc cttctacaag cgcgaatgtt tcccaagaaa atgctggcat
    20281 gttttctaat ggtaaagtca ctttgatcat tcttgccata tatggcacac ggaaacggtc
    20341 tttcggcaaa tcataaccaa attggacagt ttcttcagat aaaatagctg gtatagtggc
    20401 cccacttttt gtttctacat gatacttacg tttaggcaaa ttttctaact cttgatatag
    20461 ttgttcaggt attccttcag cgaatctttt tctttctaat tcgatggtta tttctgcttc
    20521 accagtttta tccattcctg cagtatttac aatgacaaat ggaaagctgt cttttgggaa
    20581 tccggtcgta tcgattgctg cggtcagctg ttctaaggaa tcttgtgcaa gatactttcc
    20641 aacttcatct gctttttcaa aacgaggtat catttcccga tggacagaat cgactgaaca
    20701 accgcaaata ctgtcgtgag gatgattttg cattaatgtc ttccaagcat aatctaattg
    20761 atcatgggga tagtttccac taacttcata agccatcgtc gcaagtggtt ctgtaatatt
    20821 ttctagctgt cgctggacct tggtattcca ttgttttaag taaacccgag cagatgctgt
    20881 attggctaaa gtataccagc catccgtttc ttgacttgtc aattctcctt ctactgagcc
    20941 taagtcttct ggcacatctt tttgaactgc ttccagatag tcagtaaaat tagaatgaat
    21001 aaactcgtaa tctggaaaca gttcatttgc gagatggatt gcttttgata tatctttttg
    21061 taccggttga tgatcaacac cattcatcat caataagtgg ttcgtagagg catattgttc
    21121 tgcatccgct aatttttgct tccaaaaggc aagagctgct tctttttcta ctggaatctc
    21181 attcccgttg ctataccaat tggcaaatag cagaccaaag attgctgttt gatcaggtcc
    21241 tttccaccac atctctgagt attgggaaga atagttttct gcttccaaaa cttgattatc
    21301 aaagccaatt ggttttacac cacggccaaa cgctgctgct gaaataccag cttgtttcat
    21361 taattgaggc gtctgtccca tatttccaaa tgtatcggga aaatagccta acatgacagg
    21421 ggttccccat ttacggcttt cttccatgcc aatcagcata tttctcacgt tcgattccga
    21481 gctgataaga aaatcatctt gcaagatata aaatggtcca attctcaatt ttccttcatt
    21541 gattgcttgt tggattgcct gtcttttttc tggccttact tgaagatagt catctaaaat
    21601 aatcgtttgc ccatccaaat gaaaactgtt gaaggacgga tcaacttcaa atagctctag
    21661 tagatcgtct atcaattcaa tcagtcgcat gtgatgctgt tcgtaagcca tgtaccattc
    21721 acgatcccag tggctatgag aaatgatata tactttcttt ttcatttacg atacctccta
    21781 cttttctact ctaatatcaa agtaatccat gactaattcg caaaacatca tatttgccca
    21841 agaaaaccat tctctcgtat aattttcagg attgttcaca tcgaatcctt cgtgcataag
    21901 gtgtgttcct gcgtcagttg ctacaagtaa attcaatatc cgctcttttt cagatttgtc
    21961 tgacgttgtc attccttcca tagctaatgc aatcggccaa acgtattttt ctggcgtatg
    22021 ggaggatcca attccttttg cgtactttcc ttcatagaaa tatggatttt ctttactcaa
    22081 caatgttctt cttgtttgca gataatcttc ctcttgttca gaacaatatc ccaagtaagg
    22141 tgcggcgatc agattgggca cattactgtc atccatgact gtggcgtttc ccattccatc
    22201 tacttcatat gcataaatcg tttgcccttt ttgattaacc gttcgtccgt gattctgaat
    22261 cccctcttgg atctctttct tcaatttctg tgtttgctgt acaatttctt tatcatcgag
    22321 tatcgtttga aaaatttctt ccacataacc taacacgacc accgcaaaca tattggatgg
    22381 taccagatat ccataacggc aagtatcatc gctaggacgg aaaccagacc atgtcattcc
    22441 tgtaaaagct gtttttgcac ctcgcccgtc attgatcagc gtatcttctt ttcttgtcgt
    22501 gtctcgtaca aatgtataag gagagtgttc atgatcttgc tctgttttga acacgtccaa
    22561 gatttttttg ataccttcaa taaaagattg attgaattga tcagtttttc ctgtattttt
    22621 gtaaagaaga taggccaact gtattgggta gcataaagaa tcaatttcat attttctttc
    22681 ccagatccaa tcgttcattt ttgtatgatc tgtttggtgg ccagcaccgt tagcttcttc
    22741 gttgaaggca ttcgcatatg gatcgatatt gatataaaaa aattgctttt tgactaaccc
    22801 gctgatcatg tcagctaatt cctcatcttc ttttgcaatc actaaataag gacgaacttg
    22861 tgcagtagag tctcttaacc acatcgctgg aatgtctccc gtcaataaaa aggtggttcc
    22921 gtcattttgc tttttgactg ttgtcaacag agtattggca aatgctgctg taaaattctt
    22981 tgcccagtcc gcatggatat ccccacattt ttgtgtgata tcttccataa atttctgcac
    23041 cgattttggt atatctttat aagccactaa taaaaccttc tttctatttg atatatcttt
    23101 aaagattata cttcatacag ccaattatgt ctacgctttc aaaaaatatc tttttattta
    23161 ctttttcatc cattatggta ttatttgaat atgatatatc attaagaggt gtttatatga
    23221 atcaaccatt gtaccagaca atatttaatg atttgcaaac ggctatttta actggtgaat
    23281 tacctgtaga cagccaacta ccaactgaaa aagaactttc acgtacctat aaagtaagca
    23341 gaatcacttc caaacgtgca ttgactgaat tagagcaatt aggacttatt tatcgcgttc
    23401 gcgggaaagg cagtttcgtt aaatccgctt ctccggaaca gattcttgct tctcctacaa
    23461 aaacacgacg gattctgttt cttcttccat atgtcgctga tttgtctgtt ggtgatttta
    23521 gcaagggatt gaaccctgtg atgcaagata aacagattga tgtcatgatg acgacgcttg
    23581 actttctaca aaataaaaca gcaaaagagc taatcaatga atttgacgga ttgatttatt
    23641 atgctcttga tacagaacaa catcttgact tactttttga gctttcattg aaagaattcc
    23701 ctgtcgtcat attagataaa aaaatctatg agttaccttt tcctaccgtt ctttccgata
    23761 atttccaagg cggtgcgctt gctgcctctt atttgatcga acagggacat tcaaaaatcg
    23821 cttatttgtt tggtacgcag actcatccac aatccgttcg acagcgttac cttggttacc
    23881 ttgaagcatt ggacaaagct ggtttgactt tccacacttc actggatgac aaaagcgcga
    23941 cattagatga attgctttct tatatcacta agtatgaagt aactgctttt gtttgtgaaa
    24001 acgatgttgt cgctatccag accatgaatc tcttgagaca gcatggttat catatccctg
    24061 aagatttttc aattatcgga tttgatgata tccaagcagc agctcttgtt gacccgccac
    24121 tgacaaccat cgcacaagat tttgaacagt taggtaagct tgcgggagat gctttggtca
    24181 cttggttaga gacaaaagat cgaccatccg atagtaaatt tccagtttca ttcatcaaaa
    24241 gacaatcaac aaaggagaaa taaaatgaat atcggtcaaa tagatactag acatgcaaca
    24301 gcgaatcagc caaatttctc taatgggaac tgccagcctt atactggcgt tccttttggg
    24361 atgaattatt ttgcaccgca aactacggat caaaacggca gctggtggtt tcatcctgat
    24421 gaccgcgttt ttcaaggcta ccgtcttacc catcaaccaa gtccttggat gggtgatttc
    24481 agtcatatgc tgtttacgcc gatcaatgga aaactacagg agaacaccct tttccatgct
    24541 caaagttctt accgcccaga agaaagtata ttttgcccga ctcatctgtc gatctgtcaa
    24601 ttacgttatg gcatctgttc tacattgata cctagcatgt atggcggaat cttatctatt
    24661 gattacagca aaaatgacag cggtttactt ttatcctttc cgggacgcca tcagctcttt
    24721 gttatagatc cttacacagt ggaaggaaaa atcacacatt ttgctggttc agaagatcct
    24781 gatttttctt tttattttat attgagattt gaatatcctt taacaattga tgagttagct
    24841 gtttccaaca aaaatagcga ctcgatcccg ttgtattttg aacagacaaa aaaacaaact
    24901 atccgatttg ggacatcttt tatcagctcg gaacaagcag cttataacct ggaactagaa
    24961 caaaaccaaa gcaaagagac ttaccttgct cacagtcaag aacaatggca gtcctatttt
    25021 gatcgtatac agctcacaca ccatgatcaa caacaattgc ggacgtttta tcataattta
    25081 tatcgggtgt ttttatttcc acaaacattt tatgaaattg atcctagtgg gcaaaaaatc
    25141 cactatgata cgactagtcg atctataaaa cctggtgttc tctatacaaa taatgggttt
    25201 tgggatacgt ataaaacggt ttatcctctt tattctttga ttgctgtaga aaaatacgaa
    25261 gaaatgctgg aaggtttttt gaacagctat agagaaagtg gctatttgcc aaaatggctg
    25321 tctcctgatg aacgtggact tatgcctggg acattgatcg atgcggtcat tgcagatgct
    25381 gctgtaaaag atatccgacg agacttgatg cctgaatttc taacggcaat ggaaaaaggt
    25441 gctacggttc aaagcaaaca tccaaattat ggacgccaag gaacagaaga ttacttgaca
    25501 tatggctatg ttcctaatca ctaccacgaa tcagtcaatc acacattaga ctatgcttat
    25561 agcgattttt gtattagcca agttgccaag gtactagaag aagaggaaat gacgaagaaa
    25621 tatcagcagc aatcattgaa ctatcaaaat atctttgact atgagtcagg atttatgcgt
    25681 tcaaaagata aagatggcaa gttcagagaa ccattttcag ctaatcgttg gggacaagat
    25741 tatgcagaag gaagtgcttg gcaaagcagc tttgctgttt atcacgattt ttcaggactg
    25801 atcggaaaat atggtggcag tgagagatat aaagaaaaac tcattgagct gtgcaatcag
    25861 cccccaacgt ttgacccagg aggatatggt tttgagatcc acgaaatgag tgagatggcc
    25921 gcgatagaat ttggacaatt agctatttca aaccagccaa gttttcacta tccttacttg
    25981 ttcagttata taggacaacc tgaaatggca cagccactgc ttaaacagtt gatgactcag
    26041 accttcgacg attctccaat gggttaccct ggagatgagg acaatggaag tatggccgct
    26101 tggtatatct tcaatagtct gggattttat cctgtcacgc ctggaaccgg tgaatatgtt
    26161 atcggtatgc cattaattga caaggctgtt ttgcttttgt caaatggaaa ggaattgacg
    26221 attgaagcaa cgcctaatcg gccgcagcaa caatttatcc ataaaatcaa acggatggac
    26281 aaagaatata cgaaattata ttttacccat caagatttgc tgaaaggcgg cacgatctct
    26341 taccaattag ggatcatccc tcaccctaag aaatatcaag caaaagattt gcctttttcc
    26401 ttatcaaact ataattaaca agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    26461 cagccaagca aaaatagatc ctcatatttt tcgtttggtt ggtcttatta ccgcagatgc
    26521 aagcttttga acaccgttta ttgagaggtt gtgaaatcag cgttctgacc tgagtattag
    26581 agagacaaat ggcaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    26641 cagctgattg aacaccgttt agtggagagg tcgtgaaaaa gctg
//
LOCUS       DACERL010000035        26651 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.036, whole genome shotgun sequence.
ACCESSION   DACERL010000035 DACERL010000000
VERSION     DACERL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26651
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.036"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(26..328)
                     /locus_tag="IXF54_001497"
     CDS             complement(26..328)
                     /locus_tag="IXF54_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1404763.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IXF54_001498"
     CDS             complement(353..2446)
                     /locus_tag="IXF54_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1404764.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IXF54_001499"
     CDS             complement(2935..3699)
                     /locus_tag="IXF54_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1404765.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IXF54_001500"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IXF54_001500"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1404766.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IXF54_001501"
     CDS             complement(4704..5780)
                     /locus_tag="IXF54_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1404767.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IXF54_001502"
     CDS             complement(5804..6397)
                     /locus_tag="IXF54_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1404768.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IXF54_001503"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IXF54_001503"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1404769.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IXF54_001504"
     CDS             complement(8067..8366)
                     /locus_tag="IXF54_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1404770.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..8878)
                     /locus_tag="IXF54_001505"
                     /pseudo
     CDS             complement(8468..8878)
                     /locus_tag="IXF54_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(8878..10533)
                     /locus_tag="IXF54_001506"
                     /pseudo
     CDS             complement(8878..10533)
                     /locus_tag="IXF54_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10520..12157
                     /locus_tag="IXF54_001507"
     CDS             10520..12157
                     /locus_tag="IXF54_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1404771.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLGYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATALTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPENAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(12302..13042)
                     /gene="truA"
                     /locus_tag="IXF54_001508"
     CDS             complement(12302..13042)
                     /gene="truA"
                     /locus_tag="IXF54_001508"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1404772.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEVPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(13233..13868)
                     /locus_tag="IXF54_001509"
     CDS             complement(13233..13868)
                     /locus_tag="IXF54_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1404773.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(14015..15256)
                     /gene="sstT"
                     /locus_tag="IXF54_001510"
     CDS             complement(14015..15256)
                     /gene="sstT"
                     /locus_tag="IXF54_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1404774.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            16465..18483
                     /locus_tag="IXF54_001511"
     CDS             16465..18483
                     /locus_tag="IXF54_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1404775.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVGAFFYCFIFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKANEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYSNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(18543..19253)
                     /locus_tag="IXF54_001512"
     CDS             complement(18543..19253)
                     /locus_tag="IXF54_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1404776.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATIATTTTTS
                     TESKPTAASTETADTSTSSESSKATETTQTSTSSSSVEASSSESSEQVEQILWNADKS
                     AQLEAFMATWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSE
                     SGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNET
                     ANNELKNGFNQIVYGQAN"
     gene            complement(19480..20022)
                     /locus_tag="IXF54_001513"
     CDS             complement(19480..20022)
                     /locus_tag="IXF54_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1404777.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIDNEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNMFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20027..20254)
                     /locus_tag="IXF54_001514"
     CDS             complement(20027..20254)
                     /locus_tag="IXF54_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ1404778.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(20333..22213)
                     /locus_tag="IXF54_001515"
     CDS             complement(20333..22213)
                     /locus_tag="IXF54_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1404779.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYMTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLVALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFAKIDTFVFDKTGTLTKGTTAVSTIKNYTENE
                     GDSLALVARLETLSDHPLGRAVIHYVQEKDIDFQQLAVKDNQTIKGQGITAEIDGHAV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSFVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(22403..23017)
                     /locus_tag="IXF54_001516"
     CDS             complement(22403..23017)
                     /locus_tag="IXF54_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1404780.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMGNELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKIKGIGTQLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            23159..23719
                     /locus_tag="IXF54_001517"
     CDS             23159..23719
                     /locus_tag="IXF54_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1404781.1"
                     /translation="MKKTDRRVKKTEKALAEALSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(24024..24338)
                     /locus_tag="IXF54_001518"
                     /pseudo
     CDS             complement(24024..24338)
                     /locus_tag="IXF54_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(24592..26232)
                     /locus_tag="IXF54_001519"
     CDS             complement(24592..26232)
                     /locus_tag="IXF54_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404782.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKRFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITTIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIVNSEGLVSIEGTQGETIIEV
                     SSIYPPTMKLAFLISLCSYLFLLISFLIRKLQHIKIMI"
BASE COUNT         8151 a         5246 c         4309 g         8945 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattc
     8881 cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt ttacttttac
     8941 ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt tgcttgccac
     9001 tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg attctcctac
     9061 ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg gggcgtaatc
     9121 gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat tgataacaat
     9181 atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta tagacgtgac
     9241 gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat tcatagtctt
     9301 tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac ctaagatacg
     9361 ccaaggaaac tggagcaagc ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt
     9421 agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt ttttctccat
     9481 ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta
     9541 tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa gtgcttctag
     9601 ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct gcaacatagg
     9661 ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta aattcacgac
     9721 cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta taatcataca
     9781 agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa gtagacttat
     9841 actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat aagcataata
     9901 taaaacagct ggaataatcg taagagcgat tgcttctgca attgcggatc gataatagat
     9961 atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag cagcatctat
    10021 ctttttaaaa aacttttctc cataataaaa actaatcaga atcgtacaaa tatttaataa
    10081 ccaaatatat agaacataac ttaatattaa gttatttgaa attccataaa aaacgactgc
    10141 aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt
    10201 tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa
    10261 agataaatca cctgttgtat aaatttcttg ggcatttctc ataaaattta ctgaatataa
    10321 agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt tcatagattt
    10381 gttgttctcc ttttttaatt ctattttcag tatactagaa ttgtcttttt ttaataagct
    10441 ttgcgttaaa tttttcatag cactagtatg atatactaga aaagaatcaa tttttattac
    10501 ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat
    10561 gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag
    10621 tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag
    10681 ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc
    10741 atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttagg
    10801 ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt atttttctat
    10861 gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc
    10921 cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac
    10981 atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg
    11041 gtactggttg tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat
    11101 ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat
    11161 cgctagactc ttaagtcttt taaaagctac cgccctcacc ttttttttaa gtgcgggatt
    11221 cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga
    11281 attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata actataacaa
    11341 ctatactatt ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa
    11401 attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac
    11461 caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg
    11521 gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta
    11581 tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat
    11641 attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat
    11701 agcacctgaa aacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa
    11761 agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga
    11821 aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa
    11881 taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat
    11941 taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc
    12001 tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag
    12061 atatttatct atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca
    12121 aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag aaagactttt
    12181 tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat ttgcttcata
    12241 cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta tcaaagtatt
    12301 tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt tctcctgcat
    12361 gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg gcacctgtgc
    12421 cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat ccgtctccga
    12481 caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca atcgtacgca
    12541 ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta
    12601 atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa taaaagctgt
    12661 ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg tattttttgc
    12721 ccaccgcatt atatcttgaa tggaagcgct caggcacttc ttcgattctt ttgatgacaa
    12781 tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca atttctgtgt
    12841 ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc
    12901 caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt tttccttgaa
    12961 tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg tcatattcga
    13021 ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt ggtcttctct
    13081 ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga cagaagctat
    13141 acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc ggatgaagta
    13201 tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa gatactcaac
    13261 caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc
    13321 agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc
    13381 aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc attttttcga
    13441 tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa acctgatccg
    13501 atattatgga cccatgtcga gacattccac gaagattcat gggtattgat actgaacaaa
    13561 cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac tagggtagct
    13621 aatacacgag atgtacttac aaatgcagca tacaatgcag gcaatgacag gatgaaaaag
    13681 atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga aacctctcca
    13741 aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc taatacgtac
    13801 ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa
    13861 aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat tataaacgac
    13921 tttatcttga tttaacagaa acctctattt acacgaacat ctatataact cattaaaaac
    13981 tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat ccatctaaac
    14041 aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct cctattgcag
    14101 taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg ataacaccga
    14161 taataaatcc aataccaacg atttgcatcg cgacatcatt agagatacca aacaaactac
    14221 atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca caagcagcta
    14281 aagcagacaa aatacataaa aggactttca aaacaaatgg tgcttcaatc cctaaagaat
    14341 gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata ttgatcgtag
    14401 cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt tcagccaaca
    14461 tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt
    14521 ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa aacgtgagga
    14581 atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt aaaagctgca
    14641 gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc aatcctaaaa
    14701 tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct atagatatct
    14761 gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc aacaatacag
    14821 cccagaataa aacgccaagg taattcgcat caataagaga ttgaacagga ttttgtgtga
    14881 cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt tcagatactg
    14941 tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct acaagtgcag
    15001 aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc ttattcccaa
    15061 ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt gcgattgcct
    15121 tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga
    15181 taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac gacatatcca
    15241 aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt atcattttat
    15301 gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa ccatttcttg
    15361 ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag ttaggatgtt
    15421 attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg
    15481 tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat ctttgtcgct
    15541 tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc tacatatgca
    15601 acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt tattggtaca
    15661 aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta
    15721 cattatttaa gtcgttttgc ctttatattc ctaacagcca cactagcttg caatatcttt
    15781 ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc
    15841 agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat
    15901 acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact attattttta
    15961 acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat
    16021 ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg
    16081 aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta
    16141 ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca
    16201 gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt
    16261 ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct
    16321 gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct
    16381 acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag
    16441 ttttttattt actatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa
    16501 aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta
    16561 acacaacgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt
    16621 ggagcatttt tttactgctt tatcttcgtg cagattttcg ttttagaacc aaaagaaggt
    16681 tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc
    16741 ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt
    16801 tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc
    16861 aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct gaccggattg
    16921 tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc
    16981 ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag
    17041 attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc
    17101 tttttcatta atcataaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac
    17161 aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta
    17221 aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat
    17281 ggaataatga aaaagtacaa cacaaaggca aatgaaatca accaaacaag aagtgactgg
    17341 ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc
    17401 cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga
    17461 acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa
    17521 ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag
    17581 ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc
    17641 atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt
    17701 gacaggttct attatttaga cagtccggat aaattaactt atacggataa aatagaacaa
    17761 agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat
    17821 acagaaacgt cgcaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat
    17881 tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa
    17941 gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc
    18001 caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc
    18061 gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat
    18121 ttcatttata gcaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt
    18181 gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac
    18241 tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa
    18301 agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc
    18361 caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa
    18421 tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aataaaagac
    18481 taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc ttgtttatct
    18541 cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc attattcgct
    18601 gtctcgttaa agtaaagata attatcggga ttcccctgat tttgttgtgt caccaatact
    18661 tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata tggttctgtc
    18721 tctgcatcgg aatagacggc tgtgatttga taatctgctt gacctgtccc actttctgac
    18781 cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt caaaatagct
    18841 tgagggactt taagaccata taagtccaca ctcatttgat tcgtatagct tttgtattgt
    18901 tgtcccattg tttggcccca tgtagccata aatgcctcta attgcgcgga tttgtctgca
    18961 ttccacagta tttgttcgac ttgttcgctg ctttctgaag atgaagcctc tacagaagaa
    19021 cttgaagtag atgtttgagt tgtttcagtc gctttggaag actctgacga agtagaggta
    19081 tctgctgttt ccgtacttgc tgcagttggc ttgctctctg tgctagttgt tgtagtagtt
    19141 gcaattgttg ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta
    19201 caaccagcta acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa
    19261 actcctttcg attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat
    19321 tttttgtatt ttctcatcca tcttttaaac cttttaaaaa ctaagaagca cccgctgatg
    19381 ttgtctgtaa tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta
    19441 cctcacgctt tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc
    19501 atccaaatct tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt
    19561 attgaaactt aaaagttcaa cgagtaattg ttgcaagcca tatttttctt ctttttcagc
    19621 taacttgatt gcccgtgtga taaacatatt ttggaaatca aaatcttttg caaagttttc
    19681 caacattgtt gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg
    19741 catgacttct tctaaagtag acggaacgat ttcattatca atcactaact catgagagat
    19801 tttattgaac catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg
    19861 cgaagatgta cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa
    19921 atttgctaat acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc
    19981 ttgttttgtt tcttgagcaa atttttcttc cactatattc actgatttac gcctctttta
    20041 cttttgttga ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt
    20101 ggacttcatc tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat
    20161 tgcttacccc aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag
    20221 ctagtgtatc taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga
    20281 tagtcttatt ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt
    20341 tttcttcttt gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca
    20401 agatactagc ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc
    20461 cgatgagtaa aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa
    20521 cagttttctt agccaagcgg taggcatgaa caaacgatcc aaaactagat tgcatcaaga
    20581 caacgtcact cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag
    20641 ccgcagccaa agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt
    20701 gttcttggta ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt
    20761 catcaatgcc taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca
    20821 tcactaactg ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc
    20881 gaatcacatc tgatacacct atgatttgag tgattttttt gtcgattgct acaataatga
    20941 ctgagctgcc tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtaagagaaa
    21001 gtttgtgagc ggcaatcaat ttggtgttgc ccgcacaaac tgcatgacca tcaatctcag
    21061 ctgtgatgcc ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa
    21121 tgtctttctc ctgaacataa tggatcacgg cgcgccccag aggatgatcg gataacgttt
    21181 ctaatcgagc cactaaagct aagctgtccc cttcattctc tgtatagttc ttgatagtgg
    21241 atacagcagt agttcctttg gtcaatgtac cggttttatc aaagacaaaa gtatcgattt
    21301 tggcaaaagt atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac
    21361 cgattcctgc tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa
    21421 ccaaaacagt gattgctaaa cgaaaatcac gactgatcag accaacaagc aacgcaacaa
    21481 gtaaaacggt tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt
    21541 tggtatcttg tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga
    21601 ccttagttgc ttcgatcgtc atatagccat ctgctaaaat cgttccagag aagacttcat
    21661 ctccactgat tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcataac
    21721 cttttccgtc aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacaa
    21781 tatcgccttc ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa
    21841 cactagcagt cgatggggcc atctcggtca aagccttgat tgattcccgc gttttggtca
    21901 atgttttttg ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct
    21961 tgtattctcc gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga
    22021 taactttgac tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag
    22081 aagctatggc tagagcaatg ttatagaaca agacaaatcc agtaaatttt ccgataaaac
    22141 caaaaataat caacacagct gataaggcgg tgatatgatt tcggtgctct tggatccact
    22201 tttgaaattt catctttctc cctcttttcc ttttcgttct tacgagacta agtatagaga
    22261 cttatccatc ataaaaaatt gaccactatc aagtttagtc aaaaaggcgg ttttcttttg
    22321 gtagaatata ctacaaaaaa tagttagcaa gaagactcac taaaagtgat cttgccaact
    22381 attgtcgttt ctatcaaaat tttcattgaa tgattttcct gtacagcata catcggagat
    22441 ctatttcttt atctaagatt tttatcttgc taattttttc ctttagtctt tcaaatccac
    22501 gtcgttcata gaattgatac gtacagccgc tatctgtaaa aaggaatatt tcttttcctt
    22561 tctctctctt ttcaaattct gttagcaact gggtgccgat gccttttatt ttcaaatcag
    22621 gatttgcagc taaaaaaatg atctctccat ctggcgaatg cttctttaaa tatgctgcaa
    22681 gcatttgttc atttgctgtc tcgtaagctc ctttactttc tttagcaaat atattttgta
    22741 aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga acgatgtttc tttttttctc
    22801 cttttatctc cgccagcaaa acacctgcca actcgtcctc tgcatacaaa gcaagaattt
    22861 gagttgctcg attcaactcc atatacaaaa aatatcttcc gtaaagattc agcagaagtt
    22921 catttcccat atatctctca aagttcattc cttcaacagc aaaatagata gcttttttgt
    22981 aatctttctt ttccaagggt ttaaaagtta gtttcatcat ttcgcctcct caacttcaat
    23041 gattgtgtta tacttacatt ataatagaca cagtgtctac tatctataat caatcatcta
    23101 taagattgtc gattaagcga cagctttgct ttgatatgtc ggataaggag ataggtaaat
    23161 gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct ttggctgaag cactatccac
    23221 attattagta aataaaaaga ttcaagcaat cactatacgc gaactaacag aaacggcaga
    23281 tgtacatcgt tcgacttttt atacacatta taaggatatc tatgatctat atgatcaatt
    23341 agaaagcaac ttttttcggg atctaaatga aattctctct tatgatccag cccatagcta
    23401 tgaggaacta tacactcgct tgattgacta tgtttacgca aatacttttt tatatcgctt
    23461 atttatcctc aaccatggtg atatttcgtt tcagaagaaa gttaaacaag ttattgaaga
    23521 aaactatctg aacatatggc tatatgaaga caagaaaacg attatcacag aagaaatgag
    23581 attcttcact acgtaccacg tccaaggttg tctatctgtt ctcaatcgat gggtagaaag
    23641 caattacaag tactccaaaa aagaattatt agaactctta cagaaactga atgatcacat
    23701 agagaaaatc atgccttgaa taaaaacata ctaagcttag ttaagaagaa ccgtaacctt
    23761 ctcgtataac aactgaatct tatttagcca aattgtttac tgaagatttt ttaagtaaaa
    23821 tttctataat tcctacttat tgaaaagtag tttgtagtgc tgcttctccc tctctaagtg
    23881 atatatcttg ttgtaccaat caaattcacc tcatcattaa taaattactt ttaggctaat
    23941 ttattacaac ttataggttg aagctgtaac caatttatag tcatattata tttttatcat
    24001 ccagtttatt gaattcttct cgtttacctc aattcatttt caagtggaat cagctcatca
    24061 tctaacattt tgcgccatgc ttgcttcatt tcttgtgggt atttaaatgg cttcgcttct
    24121 ggatgtttag ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga
    24181 attggatctt cacctggcat atattgcaga ttagaaacat gtaatttttt ctcagaaaca
    24241 aaccactcat tggaaaagac atgaatcaaa tcatcactta cttgtgtaaa atagcgacgt
    24301 ttaatgacaa agatatcttc attgctatct agtgtacctt catttctttg tgcttagatt
    24361 caaagataat tttctcaata aagatttttg attccagtaa gattccttta ctccttttac
    24421 aacatagcgt tgtcctttgt tttgaaacat cttcttcccc tctttgccat taaaaatagc
    24481 ccaattctct ataaagaaga ttaagcttca ttattataat ataaagttta aatttggttt
    24541 attacttact ttttcttatt ctctagtaag ctaatattga aaaaaatgta atcagatcat
    24601 tatttttata tgttgtaact tcctgataag aaaactaatt aaaagaaaga ggtaactaca
    24661 taaactaatt aaaaaagcta acttcatagt tggaggataa atggaagaaa cttcaatgat
    24721 tgtttctcct tgtgttcctt caatcgacac tagtccttca ctattaacaa tctctctttt
    24781 ttgtccattt acttttgcga ctgtccccca ataatcaaat acaggaagcg ttattttttg
    24841 tcctttagat tcatgtactg tatagactat tttcgaatcc atcacttgat aaggcatagt
    24901 atgccattcc ttgccaattc ttacttcatg attctgcaaa tgttctttgt attgtaatgc
    24961 ttccgtgggt gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt
    25021 ttcttgattc attaccaagt gatctctctg agtgacaact ttatgttcat agtttgcttt
    25081 tgttgcagca gatacagcca acagtaataa aacaattgtc gttatattaa ctacttttct
    25141 ttgcttagct gatgtgctct tcatgaaaac gaacgataaa atcaacgagc caaagagtac
    25201 ttgaaatcct aagattcgcc aaggaaattg aatcatttga atgggtgtat tttgtaataa
    25261 agaccatgga aaaaatttgg tggataagag ccaagagact accgcactaa ggtaaatcac
    25321 tttttctttg cgattaaatc ttttaaaaaa gaagagtgga cagatcaaag aaataatacc
    25381 aatgattcct accgtaaagg ttgtcaggtc attattcatc gcaccaatca aactatccga
    25441 gacatttaat gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg attgaaccat
    25501 gatttgtcgg aacatcggat accaaaagta agctgttaaa agaatcgtca ttcctactgc
    25561 ctttgacaac tccacaatca accggatgct ttcttttttt cttgtttcac ttactacaaa
    25621 gccacctaag actagtaagc caacggctat tacactcata aaagtggaaa gaacatgtgt
    25681 ataaatgagt aagctcatac tgactgctaa tggaattgca catggctttt tctcataaat
    25741 cagtgcgtaa gcataataca taactatcgg tataaccgta agtgcaatcg cttcagcaat
    25801 agcagaacga tgataaatat caatcgtacg gtacccataa aatgtatata aacagctaaa
    25861 tataaaagcc gcatcgattc tttttaaaaa ttttagtcca taatgaaaca tcagcatgat
    25921 agtacaaaca ttcaatagcc aaacatataa gatataacta acgattaaat tattacttat
    25981 ccaataaaaa atgactgccg gaaaaaaggt caagtaggga taaaaataat tcactccgct
    26041 cccataatga ttaaacgtcg taaaattgat tggaccagaa aaaattgaag ataacccttt
    26101 gactctagca aaatggaagg caatatcccc cgatgtagaa atagtatgaa catttctaaa
    26161 ataattgatt gaataaagtc cagcaataat tgctaggaga atggacaata ttattttttt
    26221 ctttacttgc attacttaat tatatactcc tttcatggat tgctttatct agtataactt
    26281 aaattttaaa taaatcgtta tttattttta ctctcactta aaaaagcact aaaattataa
    26341 gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg cttaactaat
    26401 gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc gatctcttta
    26461 gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga tatgactatt
    26521 tactactact ttactatctt tcattcttct tgtctcactt atgataaggc gctgcgtatc
    26581 atgagtgaat gatattagga aaacttcata aaataaatct tttatacatt tccacttcta
    26641 gatggttatt c
//
LOCUS       DACERL010000036        26532 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.037, whole genome shotgun sequence.
ACCESSION   DACERL010000036 DACERL010000000
VERSION     DACERL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26532
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.037"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(99..1229)
                     /locus_tag="IXF54_001520"
     CDS             complement(99..1229)
                     /locus_tag="IXF54_001520"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1404783.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXF54_001521"
     CDS             complement(1878..3080)
                     /locus_tag="IXF54_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1404784.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXF54_001522"
     CDS             complement(3140..3343)
                     /locus_tag="IXF54_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1404785.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IXF54_001523"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IXF54_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IXF54_001524"
     CDS             4880..5692
                     /locus_tag="IXF54_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404786.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IXF54_001525"
     CDS             complement(6716..6868)
                     /locus_tag="IXF54_001525"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404787.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IXF54_001526"
     CDS             complement(7059..7937)
                     /locus_tag="IXF54_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1404788.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IXF54_001527"
     CDS             complement(7976..9094)
                     /locus_tag="IXF54_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1404789.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IXF54_001528"
     CDS             complement(9091..11190)
                     /locus_tag="IXF54_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1404790.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IXF54_001529"
     CDS             complement(11241..12062)
                     /locus_tag="IXF54_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1404791.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IXF54_001530"
     CDS             complement(12059..12868)
                     /locus_tag="IXF54_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1404792.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IXF54_001531"
     CDS             complement(12889..13383)
                     /locus_tag="IXF54_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1404793.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IXF54_001532"
     CDS             complement(13395..13883)
                     /locus_tag="IXF54_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1404794.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IXF54_001533"
     CDS             complement(13960..16656)
                     /locus_tag="IXF54_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1404795.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IXF54_001534"
     CDS             complement(17084..18157)
                     /locus_tag="IXF54_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404796.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IXF54_001535"
     CDS             complement(18154..18981)
                     /locus_tag="IXF54_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404797.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IXF54_001536"
     CDS             complement(18982..19788)
                     /locus_tag="IXF54_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404798.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IXF54_001537"
     CDS             complement(19800..20885)
                     /locus_tag="IXF54_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404799.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IXF54_001538"
     CDS             complement(20902..21444)
                     /locus_tag="IXF54_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1404800.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IXF54_001539"
     CDS             21702..22691
                     /locus_tag="IXF54_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1404801.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IXF54_001540"
     CDS             22688..23425
                     /locus_tag="IXF54_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1404802.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IXF54_001541"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IXF54_001541"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1404803.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IXF54_001542"
     CDS             23989..24621
                     /locus_tag="IXF54_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1404804.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IXF54_001543"
     CDS             24706..25353
                     /locus_tag="IXF54_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1404805.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IXF54_001544"
     CDS             25358..26341
                     /locus_tag="IXF54_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1404806.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8114 a         5603 c         4499 g         8316 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tt
//
LOCUS       DACERL010000037        26027 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.038, whole genome shotgun sequence.
ACCESSION   DACERL010000037 DACERL010000000
VERSION     DACERL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.038"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IXF54_001545"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IXF54_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1404807.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IXF54_001546"
     CDS             808..1242
                     /locus_tag="IXF54_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1404808.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IXF54_001547"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IXF54_001547"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1404809.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IXF54_001548"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IXF54_001548"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1404810.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IXF54_001549"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IXF54_001549"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1404811.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IXF54_001550"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IXF54_001550"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1404812.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IXF54_001551"
     CDS             9513..10439
                     /locus_tag="IXF54_001551"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1404813.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IXF54_001552"
     CDS             10621..11514
                     /locus_tag="IXF54_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1404814.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IXF54_001553"
     CDS             11648..11773
                     /locus_tag="IXF54_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1404815.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IXF54_001554"
     CDS             complement(11907..12539)
                     /locus_tag="IXF54_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404816.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IXF54_001555"
     CDS             complement(12687..13229)
                     /locus_tag="IXF54_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1404817.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IXF54_001556"
     CDS             13364..13753
                     /locus_tag="IXF54_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404818.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IXF54_001557"
     CDS             13757..14521
                     /locus_tag="IXF54_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1404819.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IXF54_001558"
     CDS             14650..15597
                     /locus_tag="IXF54_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1404820.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IXF54_001559"
     CDS             15727..16467
                     /locus_tag="IXF54_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404821.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IXF54_001560"
     CDS             complement(16758..17426)
                     /locus_tag="IXF54_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1404822.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IXF54_001561"
     CDS             17560..17847
                     /locus_tag="IXF54_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404823.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IXF54_001562"
     CDS             18263..19801
                     /locus_tag="IXF54_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1404824.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IXF54_001563"
     CDS             19798..20763
                     /locus_tag="IXF54_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404825.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IXF54_001564"
     CDS             20750..22072
                     /locus_tag="IXF54_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1404826.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IXF54_001565"
     CDS             22313..23056
                     /locus_tag="IXF54_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1404827.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IXF54_001566"
     CDS             23071..24063
                     /locus_tag="IXF54_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1404828.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IXF54_001567"
     CDS             24075..24812
                     /locus_tag="IXF54_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404829.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IXF54_001568"
     CDS             24802..25524
                     /locus_tag="IXF54_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1404830.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="IXF54_001569"
     CDS             complement(25608..>26027)
                     /locus_tag="IXF54_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1404831.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8954 a         4426 c         5428 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACERL010000038        25768 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.039, whole genome shotgun sequence.
ACCESSION   DACERL010000038 DACERL010000000
VERSION     DACERL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25768
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.039"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            58..261
                     /locus_tag="IXF54_001570"
     CDS             58..261
                     /locus_tag="IXF54_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1404832.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            258..554
                     /locus_tag="IXF54_001571"
     CDS             258..554
                     /locus_tag="IXF54_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404833.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            631..1044
                     /locus_tag="IXF54_001572"
     CDS             631..1044
                     /locus_tag="IXF54_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1404834.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            1057..1443
                     /locus_tag="IXF54_001573"
     CDS             1057..1443
                     /locus_tag="IXF54_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404835.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            1935..2007
                     /locus_tag="IXF54_001574"
     tRNA            1935..2007
                     /locus_tag="IXF54_001574"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1968..1970,aa:Lys,seq:ttt)
     gene            2393..2653
                     /locus_tag="IXF54_001575"
     CDS             2393..2653
                     /locus_tag="IXF54_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404836.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            3258..4091
                     /locus_tag="IXF54_001576"
     CDS             3258..4091
                     /locus_tag="IXF54_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAQ1404837.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            4084..5499
                     /locus_tag="IXF54_001577"
     CDS             4084..5499
                     /locus_tag="IXF54_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAQ1404838.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            5511..7040
                     /locus_tag="IXF54_001578"
     CDS             5511..7040
                     /locus_tag="IXF54_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1404839.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            7132..8010
                     /locus_tag="IXF54_001579"
     CDS             7132..8010
                     /locus_tag="IXF54_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1404840.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            8012..8329
                     /locus_tag="IXF54_001580"
     CDS             8012..8329
                     /locus_tag="IXF54_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404841.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            8378..9079
                     /locus_tag="IXF54_001581"
     CDS             8378..9079
                     /locus_tag="IXF54_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1404842.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            9094..9984
                     /locus_tag="IXF54_001582"
     CDS             9094..9984
                     /locus_tag="IXF54_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1404843.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            10007..10222
                     /locus_tag="IXF54_001583"
     CDS             10007..10222
                     /locus_tag="IXF54_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404844.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            10234..10575
                     /locus_tag="IXF54_001584"
     CDS             10234..10575
                     /locus_tag="IXF54_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404845.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            10575..10946
                     /locus_tag="IXF54_001585"
     CDS             10575..10946
                     /locus_tag="IXF54_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404846.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            10939..11334
                     /locus_tag="IXF54_001586"
     CDS             10939..11334
                     /locus_tag="IXF54_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1404847.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            11336..11713
                     /locus_tag="IXF54_001587"
     CDS             11336..11713
                     /locus_tag="IXF54_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404848.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            11714..12322
                     /locus_tag="IXF54_001588"
     CDS             11714..12322
                     /locus_tag="IXF54_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1404849.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            12322..12663
                     /locus_tag="IXF54_001589"
     CDS             12322..12663
                     /locus_tag="IXF54_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404850.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            12905..15694
                     /locus_tag="IXF54_001590"
     CDS             12905..15694
                     /locus_tag="IXF54_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1404851.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            15684..16424
                     /locus_tag="IXF54_001591"
     CDS             15684..16424
                     /locus_tag="IXF54_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1404852.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            16421..19183
                     /locus_tag="IXF54_001592"
     CDS             16421..19183
                     /locus_tag="IXF54_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1404853.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            19196..20104
                     /locus_tag="IXF54_001593"
     CDS             19196..20104
                     /locus_tag="IXF54_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1404854.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            20104..20724
                     /locus_tag="IXF54_001594"
     CDS             20104..20724
                     /locus_tag="IXF54_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404855.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            20728..21177
                     /locus_tag="IXF54_001595"
     CDS             20728..21177
                     /locus_tag="IXF54_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404856.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            21177..21671
                     /locus_tag="IXF54_001596"
     CDS             21177..21671
                     /locus_tag="IXF54_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404857.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            21685..22032
                     /locus_tag="IXF54_001597"
     CDS             21685..22032
                     /locus_tag="IXF54_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404858.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            22025..22165
                     /locus_tag="IXF54_001598"
     CDS             22025..22165
                     /locus_tag="IXF54_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1404859.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            22265..22654
                     /locus_tag="IXF54_001599"
     CDS             22265..22654
                     /locus_tag="IXF54_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1404860.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            22651..23661
                     /locus_tag="IXF54_001600"
     CDS             22651..23661
                     /locus_tag="IXF54_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1404861.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            23776..24984
                     /locus_tag="IXF54_001601"
     CDS             23776..24984
                     /locus_tag="IXF54_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404862.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            24968..25174
                     /locus_tag="IXF54_001602"
     CDS             24968..25174
                     /locus_tag="IXF54_001602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404863.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKLI
                     ITIDKEKKLTNINVFSVAFFAVII"
     gene            25392..25465
                     /locus_tag="IXF54_001603"
     tRNA            25392..25465
                     /locus_tag="IXF54_001603"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25426..25428,aa:Ile,seq:cat)
BASE COUNT         9283 a         3592 c         5344 g         7549 t
ORIGIN      
        1 ttaaaagaat ttcgatcaag tatagacgac actttcagat tagggagttg attgacaatg
       61 aacaacagac atcgcagaat aacaaaacta agaaaacagg aactgaatgt actaaagaca
      121 aagtttgaaa aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagtgt
      181 gttgctgatg cgagtaatgc tattcgtaag tttgggattt cgatattaaa tgatgatcgt
      241 aaatgggagg aaatgagatg aaactaaaag acggatttta cgctagtagt cacggcatcg
      301 gcggtttaat gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaagtca
      361 aagtcggtga catggttcgc tgcgaagcag aagggttcat ctatccattt cgtggatatg
      421 tagaacacgt ctataatcac tcagcaatca ttcgtattga aaacacgatg gaatgtgata
      481 aatggacagc taaaagcaaa gagaatttag cagtggtgag attggtggat attgaactaa
      541 tcaatgacaa ataaaaaagc cggatcgctc cgactgattc aataaattcc acaagtttat
      601 tatatcacat aaaaggggcg gtttgacttg atgcaattgt tacgagaggt agctttcaaa
      661 cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga gcggatggca
      721 ggtcgctctt tgatagatat taagtcgccg attattacgg acatgccgaa ggcaccgaag
      781 cacggcaata aggcagaaga cgcgatcatt cagatgatgg atatagaagc agagagagac
      841 gcgattttag cggctttgat ggcgcttagt ctgattagtc gtcagatact ctactacagc
      901 ttttgtgtgc cagatagctt ctcaaactac aggattagcc gtgaagtggg ttattcagaa
      961 agaagcatac aacggatgaa gtcggaagct ctgatagagt ttgcagaagc atataaacat
     1021 gggaaaataa ttgcttataa ataggaggac tatataatgt ggaaagatta tgtatcattg
     1081 aaagagttga aaaaagatct tattttcaaa aaaatcgtag aatggtcaga gagtgaatta
     1141 attttagaag atggaaccaa aatggaagtt gtatgtagcg aatcagattg ctgcgcatgg
     1201 gccgaaggtg aattcaaaaa tgtaaagctt gatgcagtga ttacagatat aaagattttt
     1261 gataaaggta accgtctcta taatggcgat ggacatagtt cttatgccga agtcgttgtt
     1321 tatcataatc gaaatgagat tgctaaagcg gaatgtacag caaacgatgg gaatggaggc
     1381 tactattaca gtgtctgtgc tttaaaagtc aaagaaaaac tttgtgtagt gactgatgca
     1441 taaaaaatgg cggttttttg gcggaatgat ggcggttttt agctatttac cagtgatatt
     1501 atggtagtgt cgaaagatta gtgataggtc tgagacaaaa taaaatgtaa gggaggaaat
     1561 ctccctcatc gtttaattaa gcttcgatag acagcagcgg aaatattaag aataaggatg
     1621 tgaattttaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattgtta
     1681 ttatgtcact gtggcggaaa gggtagacgc ttaaaaataa ggtcaatacg tcgagggata
     1741 gccttaacgt tttatgattt gaccatgcaa ggttcgattc cttgccagcg acttagacga
     1801 aaggtagctt aaatattaac cacatcaaca tcgtcagcga gttgataaag gtaactagca
     1861 gactctagga tttaagccag tcttaccgat tagtctgtta tagtagcaag cttgctagag
     1921 gtagctcctc tggttgacgt gcagctcagt tggtagagcg cctgactttt aatcaggagg
     1981 tcgccggttc gaatccggtc acgttaataa agaacctacg gaaacaatca atcttatcgg
     2041 atgccgattg gttggctgaa ttaagcagtg tgtctgtgaa tcagttaata ggcataaaat
     2101 actagcacag acgtgtgcca ctctcaggtg taggttagga gagaaacatt agcaaccgag
     2161 ggttggaaat gggcgctcaa agtacacgag caaggcgagg tcgatagtaa tcgatggaat
     2221 cggtgtaggt tgctattaca taattgattg ggttagattg agttttggga ttcggtacaa
     2281 atgaatcgtc aaatgactca agcacaggat cggaaacgtc cctgcctgtg cattacatat
     2341 tagatcactc gttgagtggt ctttttattt tagaaaagag aggattttga aaatgaatca
     2401 tgagaagttt atcgaaaaat gcaaggctat tgtacgtgaa agaattgaga atgagattgc
     2461 tgacctaagt ggagcagtac ctgagtttag tgttttcata gtctggtcat gcaaaacact
     2521 acagaatagc aaagcattag tcagcgctag cttaaaagga gcaccgtatt ttgaaattac
     2581 gttgaacggg gacaaaggtg aaatctatgt agatacttat ctcaaaaaat caaatgaatg
     2641 tatcaaagtc tagcaggtgc taggcttttt ctttacataa aggaggaatg atctcatgaa
     2701 acaatatacc gctaaagatt tcgaggaaat gaagcgatta aagaaggact atgaagaagt
     2761 tgatatggag ctaactgttg gagtcattca acgaagactg cgggtcggat tagagacagc
     2821 aaaggctatt tacaatgatc taaatgctat tgaagagaag aatggctaat gaggaactac
     2881 tggtatatat cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt
     2941 gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatggggag agaagcagaa
     3001 ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc atggtttcta tgatgaacca
     3061 aacattcaaa ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa
     3121 atctccaatc aggacaaatc aagttagaca aagtttccga tttaaagata ttaattgaag
     3181 cagatctaat gttgaaagat attgaaaatt agaaaacaaa actcaaccta agaagattgc
     3241 gaggtggtgt gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa
     3301 tttggttaga atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt
     3361 cagattctca ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgaaaagta
     3421 acgttaccaa tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa
     3481 atgctaaagg taacaaagga aatagccgag cttctccgcc agtgggtaat aaaaacgctt
     3541 tgaaaacagg cgagtatgaa accatatttt ttgagacact aagtgatgaa gagaaggaca
     3601 tctattctag tctgaatgat aatccttctt ttgttttgtc tgaagaaata cgtctactta
     3661 agataagaca atttcgtatg atgaagagaa tccaacaagc tgaagctgga ctaaatgatg
     3721 aagaagtcga acgattgcag cagctaagaa agattaaaaa tccgattgaa aaaaatggta
     3781 aaaagctaga aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc
     3841 gcaaaattga tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag
     3901 ctaaagccat caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta
     3961 aagctcagac tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg
     4021 aagaaataga agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag
     4081 aaaatgaata atgaatttat tccttttgct gatattggtt ctgccattga ttattactat
     4141 gataaaccag tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa
     4201 aatgttttaa atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt
     4261 ggaaaaacag cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct
     4321 aaagtaatag caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta
     4381 gctaaatggt taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt
     4441 tatatggttg gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa
     4501 aatatgcaag gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg
     4561 tctgatccaa ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg
     4621 atgtgtggaa accccaataa tattgaaggt gttttttacg attcccacaa ttcagaccgt
     4681 gataaataca gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat
     4741 atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt
     4801 ggagaatttc ccaaaggtgc gttggattca tttatcagtc ttgaaacggt tgaattggct
     4861 acagaaaaac aaattagtga ttctttagtc aataaaacaa cggttgctca tattggtgtt
     4921 gacgtagctc gatatggtga tgattctacg attctctttc ctagaattgc taccagggca
     4981 ttggagtatg agaagtattc aaaacgtagc accatggaaa caacaggata tgtcatcaac
     5041 atggccaaga atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat
     5101 gacactggtg tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaaacat
     5161 tatccttttg aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat
     5221 aatttaggta ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca
     5281 aatcttaacg gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa
     5341 ttaagtactc gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat
     5401 gatatgaaaa agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt
     5461 tatgagccac caagtcacta tcaatttatt caattttagg aggtgagctt ttgattagta
     5521 cagtattatc tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag
     5581 atggaatgtt tgatccgaat ggttttatag aagatatgaa accatttttt gctgatcgcg
     5641 aaagaaaata tctagcttat actagcgaaa agaatgaaat agataataga ccaaagccta
     5701 acactgatat tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag
     5761 accaagcagt caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac
     5821 aaaaaagaac actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc
     5881 cgacagttcc agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa
     5941 tgcttgattc tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgttag
     6001 agcctgtgga agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag
     6061 cagaaagata tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa
     6121 aattttttca agaaatgaaa gttatgacga gaaataaaat tctgacatat gcttgctatg
     6181 gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt
     6241 tggggacgat taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag
     6301 tagctgagga aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg
     6361 aacaatttaa gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta
     6421 aagaaatgat gaatcagcta gggattatca acttaaaaga ccctactgca aaagctgaat
     6481 atgtcacaaa aaatttagca aaagatttca atgaatatca tatggatcta ttgaaaaaac
     6541 agttttacac catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt
     6601 ctggagaagc tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc
     6661 aatattttag agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc
     6721 atgacaaagt agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa
     6781 ctgatttaag ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa
     6841 ctattcagag ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat
     6901 tggaaagtgg aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg
     6961 acgatagaaa cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa
     7021 caagaaaatc agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata
     7081 caagcaaaac tattatcaca agctgactta cacaatatca cttatccaga aatgatggag
     7141 ttttatagta caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca
     7201 ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac
     7261 tatgcaaaag tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa
     7321 tatgcgatgg atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa
     7381 agaacatatt cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat
     7441 tatttaccag aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt
     7501 tcaagaagac tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta
     7561 aaaagtgttg ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca
     7621 gaaatttgtg acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc
     7681 gttcgaacgg agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca
     7741 ggtattgagg aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga
     7801 ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca
     7861 cctttccata gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat
     7921 attgatgaag aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct
     7981 tttggaatta atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac
     8041 aattaatgta aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca
     8101 tcctaatggc ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca
     8161 aactgttaat tatcaagata atactccagt tgataagaat accgttaatt taacgattca
     8221 attattatct cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg
     8281 atgtattgtt gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt
     8341 ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac
     8401 tattaaaact caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag
     8461 catgaaacac aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca
     8521 aaagttttct tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt
     8581 tattcagtca caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa
     8641 cttagataaa attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc
     8701 acctgagcaa attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg
     8761 tgtgacaagt gataataaag catttgttgc agaacaaatt gctggattag aactagatgg
     8821 agagttaaaa gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga
     8881 tttcactaag aatgctgttg aaggttttac aagtgtcttg aatgcaatta atgagaaaca
     8941 tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa
     9001 taaccaggtt cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat
     9061 tcttcaacaa tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta
     9121 gagtatttgg atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta
     9181 agctatttat ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt
     9241 gaatatgata tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat
     9301 gctgaatcag gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct
     9361 gtatctggta cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa
     9421 gtagcactac gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta
     9481 aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat
     9541 tcagcaaata aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact
     9601 ttatttaaat tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca
     9661 gatatgcagg agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc
     9721 ggggaaagag taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt
     9781 attgctctac tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat
     9841 gtgaaactac gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct
     9901 agacgtatcg gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca
     9961 attttaaatt taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat
    10021 tacagtggta aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa
    10081 aactaaaaat tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc
    10141 aaataacgaa tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc
    10201 aattgatgtt aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc
    10261 attgagtcta caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta
    10321 gaaataattt tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa
    10381 aagttaaaaa gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa
    10441 acagtatctt cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat
    10501 gttagtgagt tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga
    10561 ttggagtttt tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta
    10621 ttattgaaag actgacggac attgaagatt ctaatacaaa tattactatt caagattaca
    10681 aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag
    10741 cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc
    10801 atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct
    10861 taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt
    10921 tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt
    10981 gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat
    11041 tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta
    11101 aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagca
    11161 gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt
    11221 ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat
    11281 attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa
    11341 agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat
    11401 atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga
    11461 atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc
    11521 attgattgat aataaaccta atagaaattt aattaatgaa ttagtctcac gttgtgggac
    11581 tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga
    11641 tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg
    11701 gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc
    11761 tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc
    11821 aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg
    11881 ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc
    11941 aaatggatat actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg
    12001 tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa
    12061 atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga
    12121 tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc
    12181 atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt
    12241 ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga
    12301 actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa
    12361 gatgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca
    12421 gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga taaacaatca
    12481 gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa
    12541 tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga
    12601 aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag
    12661 taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata
    12721 taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg
    12781 atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt
    12841 actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct
    12901 gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt
    12961 gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag
    13021 tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc
    13081 tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa
    13141 taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat
    13201 cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc
    13261 gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat
    13321 atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga
    13381 agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa
    13441 ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat
    13501 tacaatggga gatctgaaag aaggtttgtc tcaaggtaag atttctgttg aagaattcca
    13561 aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat
    13621 cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac
    13681 acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg
    13741 tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat
    13801 tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa
    13861 atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg
    13921 agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa
    13981 gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa
    14041 ttcatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat
    14101 ggcaaccaat gaagagttta gaaacaaggt tatatctatt tggaatgatg tgaaagattc
    14161 cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa
    14221 agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg
    14281 gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt
    14341 agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg
    14401 gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atgaaattaa
    14461 aggttctatt atgtcggttg ctggtccatt gattacaggt ataaagaatg catttttaca
    14521 tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt
    14581 tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg
    14641 tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc
    14701 cataaatatt tgggaatcta taaagaatat atttgtaagt tattttacaa atatttattt
    14761 tgctgcactc aatatttgga cagggttcaa gcttacattg ataaatattt ggaatgaagt
    14821 ggtaaatcaa gctaaatcga tttggttaaa cataaagtat ttttttatta atctttggat
    14881 tgatataaaa tattttgcaa ttcagaaatg gattgaattg aagttttcta ttattcaaac
    14941 ttggatcgat ttaaaatata atgccattac tacttggaat aatattaaac agtttttcaa
    15001 agatacttgg aaaaatatta aagatacggc atacaatacg tggatttcta taaaaaatac
    15061 catgattaat acatggaata acattaagga ctctttctgg aatattgtta ctggaattgt
    15121 taattctgct gaaaatgcat ggacaaatct aaaaaatggt gtttcaaatg ctattaatcg
    15181 ggtaaaagaa atctttaatt cgttaaggga agtcaattta tttgaaattg gcaagaacat
    15241 cattgatgga cttataaatg gtgtaaaaga aaagtggaat gctttgaaag atactatcaa
    15301 aggtattgca ggtaatatca aaaattggat taaaggagct ttagatattc attctccatc
    15361 aagatggatg cgtgatatgg ttggtaaaaa tattgttcaa ggtattatcg ttggtattga
    15421 taaagagcaa agtaagttag atcaaaccat gacaaattta gtaaaaacac cgtctgttca
    15481 acctgtagta aatggaataa gtcacctacc aattacacaa acaaagcaaa atatatctgc
    15541 tagtgaaaca aaagaaattc atttacattt aaatgtttat ggagatttac ctgattcaat
    15601 ggttcggaag attaccacaa aaattaaaac agaattgaca agacaaatga aacgagatgc
    15661 tggtgcagta ggaggaacat tatatgcaac ttaagagagg acaatttttt attaatcaac
    15721 attattcttc tgaatttaat gtgtatattc aaaacaggcc tgcttctgtt tcagctagtc
    15781 gagtaattga attgagagag cgagaaggaa atgattctat cattattgat aaaggctatt
    15841 acagaaatgt gactagaaag attgagtgtt actataaagc accttcaatt gatgtggtgc
    15901 aggagtggga agatcgaatc actgaatggt tagatatgtc gtcttatagt gattttattc
    15961 tttactatga tgaacaatat atttatcaag ctgtagtaat agaaggacct gaatttaaag
    16021 gaacaagaaa aacaggaaat attgtccctt ttgaatttac agtgagcatt cgaccattta
    16081 aggaaaatta caatggtaga tttactattc gacaaacaga aacttttgaa atttataacc
    16141 cagagaagta ttcttcaaaa ccgcttatta aattgagtgg ttctggagat gcttcttttt
    16201 atattaataa ggacaaatat gatttgaaat cgttggacag agaattattt atagattcta
    16261 aattagaaga ggcttaccga aaattagatg gtaatttaga acatcaggat caagtcactt
    16321 tatttttaga ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata
    16381 ttcattcatt tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag
    16441 tcctactgaa acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg
    16501 tgaaatatat gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac
    16561 tagatttgat gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga
    16621 agagtatcat atctttttta ttgatgataa agatatagat acttttttaa atacagtaac
    16681 tatttatgct cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgcggaagt
    16741 agactctaaa actggtcaag aagcgatatc aattattgaa accaaaatgg ataaaaaatc
    16801 tgacatacga ctttattctg atattacggc cgtttctagc acaatctttg aagcgagaaa
    16861 tgttttaaat tgtattgctg gtgaacaagg atcattgctt caatattggg gtggagaaat
    16921 taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac
    16981 gattcggtat ggaaaagata tgtctggttt aaaggtcaaa ttagattgga caggtataaa
    17041 aacaagactt attccatatg ctgatcctca aagtgaggta ggtacgacta atcgaattta
    17101 tggttcgcca gtagatagtg cctatattaa taactatcct gatgtgtata cagagcatgt
    17161 tcagtttaca gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa
    17221 ttactttaaa acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt
    17281 tgataagttg actgatagtg aagaagcgaa agaatttgcg aagattagaa attatggtct
    17341 gtttgataca tttaaaatat atcataaaaa atataatatt tatcttgaat caaaggttag
    17401 tggactacaa tacgattctt tatctgaaaa aactttgaaa ttagaagcag gagacattca
    17461 agttgctttt tatcagcaac aagctgtaac tattcaggat aaattaaaag attatgcaac
    17521 gaataattat atgagtgatt ttaatgatta tgtttcttca atgattacag gtcaaggcag
    17581 tgcaggagga tatgtaagtt tatggccaaa agaaaaacct tccaacatct ttattatgga
    17641 taatccagat ttagaaaagg caagagaagt actaagaatg aataaaaatg ggattgcttt
    17701 ttctaagaaa ggatggcggg ggccttttac ttcagcttgg acattagata gtatatttaa
    17761 tgccaatttt atacaaactg gattaatcaa agcagatatc tttcaaaatt cttttaacaa
    17821 gactggagat gtattgaaat tagttaatgg actactccaa atttggaaca acaaaaagaa
    17881 aataatggag ttaactaaaa aaggaatgga attttggaat tctaagggtt caattggaac
    17941 gattggaaca actgattctg ctggtaatcc ttttcctggg gcttctactc caacccctat
    18001 tgaagataat tctttagtta ttcgtacaaa tggagacggc aaatatattt tgatttctcc
    18061 taaagtagga aaaggattag ttttattagg aaatggtaaa gcaatttatt ttggagactt
    18121 agatgtacaa ggaaaactta cagttaaagg aaaagaaata attcctggac aaggtggtgg
    18181 tccatcggga ggtggagaat ctactggtgg gtatcctagt gaattaaaaa cagatgcaga
    18241 aaagagagct tggagaattt acgatatttt gtgtaataac ggatttacga aacaatctgc
    18301 atgtggaata ttggggaaca ttcaacaaga aacaggagga acttttgatc ctgatactgt
    18361 tcaaataggt ggaccagcat acggattagt tcagtgggat ggttcttcat atcctcttgt
    18421 tggtccagca acatgggatg gaaaagttta tgttcaaaac ttatttaatg ctgcaggtat
    18481 taaagaacca ataacgagct tagatgcaca agttcgattg cttatttgga catttacaaa
    18541 tggacaatgg atgggcgtag tacaacctac gacggttgat ggatttaagg cctgtactga
    18601 tccaagacaa gcagcatatg cttttgaacg aaactatgaa cgtccggcag cgacacatcc
    18661 tgaacgtcaa gattatgcag ttaactggta taacaaattt aaagatttaa aaccaggagg
    18721 agctactgga gaagcaggac taaaacattt agagtctttg attggacaac gaattggcaa
    18781 tggtcagtgt tatggcttgt ctgcagaata ttcaggatat ctgggtggtt gtggcatggg
    18841 tgctggaaca aaatatggtt taactcatgt gataggaaat acttctgcag catctgatat
    18901 tggtattgcc tatgattggt ctgctgttgg ttggaaagtg attcaaaacc ctagatatga
    18961 tcaattagta gttggtgcaa ttattaattg ggcaagaggt ggacaagtgg gttcatggtt
    19021 tgcagacgga acttatggac atactggcgt tatccgaggc ttagctaatg gtcgtatgca
    19081 aacttatgaa caaaacacag agttaggtat gatttgtgga aagttagatc gccagtatta
    19141 tagtgcaagt gcaatttctt ccattgtcat accaccaaaa tagggaggtg atttaatggc
    19201 aaagtggaat gtcatattaa gtacaacaga accctataat tacgtgggga tgattcaagt
    19261 tcgacaaggc aataaaaaca ctgaagttat ggaagctaca attgtcgaaa atggtcttcc
    19321 atatgattta tcagaatgta aggtatattt tgaatcagtt gttggcggaa aatatccagt
    19381 ccaattagaa acaagaattg tggatgctaa aaaagggaaa atcaaatata tttttgataa
    19441 atattccatg cagtgtttgc atcgacaaac agccaatttc attatattta aaggagaaga
    19501 cttgattgga acaactcaag acttctccta ttttgtcatc aatgctgttt caaaaacaga
    19561 aggagaaatg ggttcttatt ggcaatcaat tgaagattta attgctgata tgacagattt
    19621 tattaatgaa aataaaggcg attttacgga ctggatgaat gaaaggaaag aagaatttga
    19681 tcgctggaga gaagaacagg agaatagttt ccaagattgg agagaaggac aagaatcaga
    19741 ttatttgtca tggtttgaat caatcaagga tattttgaag tctatcgatc caggtggagt
    19801 aatgttagcc gaattaatgg atgcacgtgt agatcttcaa ggtgtacgcc atgaatcgat
    19861 ttctgaacgt ttcctatccg atctaaatta tttgtatcaa aaaatgaaag cagcactttt
    19921 cacgattgaa tacggtgaaa ttgaagtgac tgatattctt caagacgatc tcttttcaga
    19981 taatcacgaa gtcgaaaaaa ttgaaacagt agaatttcca attgaagaag gagccttgat
    20041 catcgcaacc gttgatgatc caaagcaaaa tgtgttcact cttgagaaag tcggggtgat
    20101 ctaatggcta aaactaaacg aatgatggaa acggatgaaa aaacaggtgt acaacgccaa
    20161 ttctttccaa tcacacatgc ttccgcagtt cttgggttag aagaaataat agcaggagaa
    20221 gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg tcattactaa agaagatttg
    20281 ggattagaga atgttctcac agaattaccc tatgcaagtg aagaagatga cggcattatc
    20341 acggcagaaa tgtatcaaaa aattttaaac agtggagaag gtgactacgt gttgccagtt
    20401 gccactatcg accgtttggg tggcataaaa gttggtgaat tattgacgat tgatgagaca
    20461 gggaaagtct ctgcagtcag acaatctgat gtcaattttt cgctggagtt aaaagaaaaa
    20521 ctcgattcac tgaaaaatta tactgctgga gaaaacatca ctattgatga agatggaaca
    20581 attagttcta caggtggtag tgggactggt ggtgtcaatc agtcatatgt agatcaaaaa
    20641 ttccaagaag ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact
    20701 tttgaaaaaa ttggggaggt atagagaatg acagatatcg taaaaataaa acaaagcaat
    20761 gtacaggttt atccacaaac tcattggaat gctatagaag gcaaaccaac aacggtgaag
    20821 ggagataaag gcgatcctgg ccaagcagca acaattacca taggaaccgt ttctagtggt
    20881 tcaaccgctt ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa ttttgtttta
    20941 ccaaagggag ataaaggtga tcctggaata aatgccacaa ctacagctgt agctacaact
    21001 acagcaaatg ggttgatgtc gtccacagat aaaactaagt tggatggaat tgcagctgga
    21061 gcacagaaaa atccaggtaa tgctacaact acgacagcag gtttaatgtc agcaaccgat
    21121 aaagtgaaac ttgatggatt agccaatatt acatttgaga aagtggggac ggtttaatgg
    21181 ctgatattgt tcaattaaaa gaagacggag ttgctaaata cttaaaaacg catgtaaaag
    21241 ctattgatgg taaagaagca ttggtgcaga cagatggaga ccaagccatt gcaggacata
    21301 agaatttttc aggttctgta acaatcaata ataaacgtgt tttgacgact gatgataaca
    21361 gatatgaagt ggtaaatctg gttgtcacta acggtaatac agggacagca aagctttatc
    21421 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagt agtggcggga
    21481 atgattcggt tattttaact gttccagaag gctatcggac accaattagt tttgaacaac
    21541 tggttgggtc aatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
    21601 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
    21661 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
    21721 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
    21781 ccaccagttc ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta
    21841 gctactgaag aagagaagag tggcacagcg gttgacgata ttgaagatgt cgatcagttg
    21901 aagcaattaa atgctctact gacaaaacaa ttggcgatat cggtacagga acaagaaaaa
    21961 atgcaacaaa tgttagctca attgacgatg gaagtcgcaa gtattaagaa tggagggaaa
    22021 agtaatgaat agttttccgg gctttgaaaa tataaaacaa ttttatgatt ggggatgtta
    22081 tacggaccaa gatttacttg actatgtaaa catgaactgt ttaacaaaag accaatataa
    22141 gcagattact ggaaatgaaa tttaattaag cacaaagtta aataaaaagc gtactcaaac
    22201 gagtgcgctt ctttgcataa aggagaaata gatattggaa aaatatttaa atacactctc
    22261 agtaatgaca ggggtgatag gcgggacaat tgttggttta ttgggaggga tggacaatat
    22321 actacatgtt ttaatttttc ttgtgggtgt ggacttctta actggacttg ctaaggcatg
    22381 gaaattgaaa gaaataagta gtgagatagg tttcgaagga ttgttgaaga aagtcttaat
    22441 cttcgttgtg attgccgttg cggttgacgt acagaaaatc gttgggaatt ctatacctct
    22501 aagagaaata gttattatgt tctatgttgc aaatgaaggg attagttttt tagaaaacat
    22561 ttcggtattc cttcctttgc cagataagtt aaaagaggta ttccaacaaa tacgaaatga
    22621 tactgaaaat aaagaccgag gaggaaccaa atgaaaaaga aaatctttgt aggagctatt
    22681 atagctcttt ttttattgcc aataaacgcc tttgcttact cgatcaacaa tgaattcaat
    22741 ttgggcgcaa acgaaggtag ttctcaagta gcaaataacc aatacatcct attgcatgaa
    22801 acagcaaacg aaactgcgac aggacgaaat gaagcgcagt acatgaaacg ttcttggtac
    22861 aatgcctata cagcgtatat tgttggcgat ggtgggatcg tttaccaagt tggacaacct
    22921 ggttatgtac aatatggcgc tggttcatat gctaatgcaa atagtcctgt gcaaatcgaa
    22981 ctgcaacata cgcacgataa agtaacgttt gaaaagaact acaaggcata tgttgaatta
    23041 gcgagagatt ccgctaataa atacggtatt ccacttacat tagacacacc gtataatcaa
    23101 ccaggaatca aatcacattt atgggtaact caaaacattt ggggcgatca cactgatccg
    23161 tatggatact tatcagaaat gggtgtaagt aaggaaaaac tggcttacga tttagctcat
    23221 ggatttacag acgaaaaccc aacgacttct gagaacaagc ctgtcattga tccaacccga
    23281 gcaggtgcag caaatccaac tttatcagat ggaacgaacc attctcacat tgatcagttt
    23341 ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc acatcgctaa ctataaatac
    23401 gagtatattt tcattatgga ctacaatact gggaaagaat tagctcgagt aagagctgat
    23461 ggaatttata gaccggatgt aaatcaagct tataatactt ctggaaacgt tggctatcat
    23521 gtatctttta acatgcgcaa tttccctaat aagaaagtat acgtcatgat gcgggcaacg
    23581 aatgatccag aaggaaacac taaaggcggt gcgcaagatt tccatgacaa acgttggtat
    23641 ttaaatattc ctaaacgata aaaatagctc ctcgttgagg agcagtacat agaattgaaa
    23701 attaacgtta aatcaaaaaa atatttacaa gatgtttatt taagtagata ataaaagaaa
    23761 aaagcaggtg aaattgtgac agcagaaatt ggtattatga ataaaagtgg aatcgtgtta
    23821 gcttcagatt cagcatctac gattggagat agcaaagtat ataatactgc aaaaaaatta
    23881 tttactttgg attccatgca ttctgtaggt atcatgatat atggtaatgc tgaatttaat
    23941 ggtattcctt gggaaataat cattactcaa tataagaaaa gcattggtag ttctgttttt
    24001 aatactttgg aagaatacgc ggataatttt atagaatttg tcaaaacagc atcttttatt
    24061 agaagtgaac agaccgagca agaacaaatg attggggttt ttcaaaaaat aatttcaggt
    24121 ttatttgaga gtatagaagt agatattaac tttttaataa gccaaggtac acaaatagac
    24181 aaagatgtcc tagtgaagtt attacaaaca aaaatgaata ccaatttgtc tcaacagtcc
    24241 cagacattca ttttagatat tgaaaaggcg ttatttctta gtaactatgg agaaatatta
    24301 aaagatattc ttaatagtat ttctacaatg gaaggtgttt cagaagctat atcagaggaa
    24361 attcaaagtt acgtttatga aattataatt agagacgatg tttattcttc ccctacagga
    24421 atagttatag caggatatgg aagaatggat attttcccta aactatattc ttataatatg
    24481 tttggatttg ttatgaatat tctgaaatac tcagaatatg aaagtgctca aataggaaat
    24541 gacaatggtt cattaagatc tactatatta ccatttgctc agtctgatgt ggtaaatact
    24601 gtagttcaag gggtagatcc acaaattact aattatctgt cgagtcaagt cgatagtttt
    24661 gacgataacg gaaaaaatac ctatattaat attataaaga atatttcaga gtttcaacaa
    24721 aatcaattca tatttccgtt actaaacatg attgctttac tcccagttga agaaacagca
    24781 attatcgcag agactttatt aaatttaacc agttttaaac gaaaatatac tacttctgta
    24841 gaaacagtag gtggcccaat tgatgtttta gctattacac ctaatgatgg ccctatttgg
    24901 attaaaagaa aacattattt tgatattgat aataatattg gatacagatt gcgaaaggag
    24961 agagcgaatg attacaataa ttaaacctca ggaaaaatcc cctgttttga aatttaatga
    25021 gagcaatcta gcccataata aaatgagtat aactgataca gtatttgatg taatggttga
    25081 gcaacaaaaa aaattaataa taacaataga caaagaaaag aaattaacca atattaatgt
    25141 tttttctgtt gcgttttttg cagtcataat ttaaaacctc gctcaatctt gggcgaggtc
    25201 tttttttatt tttctgaaaa gttaaagtaa ataatagcat aaaataatat attttacaaa
    25261 gaataagtac aatctagttt tttgctatta aatgtgtaat aataaatatg ccatcacaac
    25321 atgaagaatg aaatccatta ttatctagtc tagtccattc tttttgtttg cagtatttgt
    25381 gatggctttc cgtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
    25441 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
    25501 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
    25561 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
    25621 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttaaat tgtgagataa
    25681 taataaagaa gagtttaaag cgcacctcaa accacttccc cataagtgtg ttacgcttta
    25741 aactctttta tatttgaagc tattaaaa
//
LOCUS       DACERL010000039        25535 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.040, whole genome shotgun sequence.
ACCESSION   DACERL010000039 DACERL010000000
VERSION     DACERL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.040"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXF54_001604"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IXF54_001604"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1404864.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IXF54_001605"
     CDS             complement(937..1518)
                     /locus_tag="IXF54_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1404865.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXF54_001606"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IXF54_001606"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1404866.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKGFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IXF54_001607"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IXF54_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1404867.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IXF54_001608"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IXF54_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1404868.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IXF54_001609"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IXF54_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1404869.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IXF54_001610"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IXF54_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1404870.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7566..7874
                     /gene="rpsJ"
                     /locus_tag="IXF54_001611"
     CDS             7566..7874
                     /gene="rpsJ"
                     /locus_tag="IXF54_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1404871.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7905..8534
                     /gene="rplC"
                     /locus_tag="IXF54_001612"
     CDS             7905..8534
                     /gene="rplC"
                     /locus_tag="IXF54_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1404872.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8562..9185
                     /gene="rplD"
                     /locus_tag="IXF54_001613"
     CDS             8562..9185
                     /gene="rplD"
                     /locus_tag="IXF54_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1404873.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9185..9475
                     /gene="rplW"
                     /locus_tag="IXF54_001614"
     CDS             9185..9475
                     /gene="rplW"
                     /locus_tag="IXF54_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1404874.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9514..10347
                     /gene="rplB"
                     /locus_tag="IXF54_001615"
     CDS             9514..10347
                     /gene="rplB"
                     /locus_tag="IXF54_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1404875.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10390..10668
                     /gene="rpsS"
                     /locus_tag="IXF54_001616"
     CDS             10390..10668
                     /gene="rpsS"
                     /locus_tag="IXF54_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1404876.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10691..11038
                     /gene="rplV"
                     /locus_tag="IXF54_001617"
     CDS             10691..11038
                     /gene="rplV"
                     /locus_tag="IXF54_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1404877.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11052..11708
                     /gene="rpsC"
                     /locus_tag="IXF54_001618"
     CDS             11052..11708
                     /gene="rpsC"
                     /locus_tag="IXF54_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1404878.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11711..12145
                     /gene="rplP"
                     /locus_tag="IXF54_001619"
     CDS             11711..12145
                     /gene="rplP"
                     /locus_tag="IXF54_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1404879.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12135..12323
                     /gene="rpmC"
                     /locus_tag="IXF54_001620"
     CDS             12135..12323
                     /gene="rpmC"
                     /locus_tag="IXF54_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1404880.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12348..12614
                     /gene="rpsQ"
                     /locus_tag="IXF54_001621"
     CDS             12348..12614
                     /gene="rpsQ"
                     /locus_tag="IXF54_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1404881.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12672..13040
                     /gene="rplN"
                     /locus_tag="IXF54_001622"
     CDS             12672..13040
                     /gene="rplN"
                     /locus_tag="IXF54_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1404882.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13077..13385
                     /gene="rplX"
                     /locus_tag="IXF54_001623"
     CDS             13077..13385
                     /gene="rplX"
                     /locus_tag="IXF54_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1404883.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13413..13952
                     /gene="rplE"
                     /locus_tag="IXF54_001624"
     CDS             13413..13952
                     /gene="rplE"
                     /locus_tag="IXF54_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1404884.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13971..14156
                     /locus_tag="IXF54_001625"
     CDS             13971..14156
                     /locus_tag="IXF54_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1404885.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14192..14590
                     /gene="rpsH"
                     /locus_tag="IXF54_001626"
     CDS             14192..14590
                     /gene="rpsH"
                     /locus_tag="IXF54_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1404886.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14622..15158
                     /gene="rplF"
                     /locus_tag="IXF54_001627"
     CDS             14622..15158
                     /gene="rplF"
                     /locus_tag="IXF54_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1404887.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15319..15675
                     /gene="rplR"
                     /locus_tag="IXF54_001628"
     CDS             15319..15675
                     /gene="rplR"
                     /locus_tag="IXF54_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1404888.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAKAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15696..16196
                     /gene="rpsE"
                     /locus_tag="IXF54_001629"
     CDS             15696..16196
                     /gene="rpsE"
                     /locus_tag="IXF54_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1404889.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16211..16390
                     /gene="rpmD"
                     /locus_tag="IXF54_001630"
     CDS             16211..16390
                     /gene="rpmD"
                     /locus_tag="IXF54_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1404890.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16434..16874
                     /gene="rplO"
                     /locus_tag="IXF54_001631"
     CDS             16434..16874
                     /gene="rplO"
                     /locus_tag="IXF54_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1404891.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16874..18169
                     /gene="secY"
                     /locus_tag="IXF54_001632"
     CDS             16874..18169
                     /gene="secY"
                     /locus_tag="IXF54_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1404892.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18231..18878
                     /locus_tag="IXF54_001633"
     CDS             18231..18878
                     /locus_tag="IXF54_001633"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1404893.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19069..19287
                     /gene="infA"
                     /locus_tag="IXF54_001634"
     CDS             19069..19287
                     /gene="infA"
                     /locus_tag="IXF54_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1404894.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19320..19436
                     /gene="rpmJ"
                     /locus_tag="IXF54_001635"
     CDS             19320..19436
                     /gene="rpmJ"
                     /locus_tag="IXF54_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1404895.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19454..19819
                     /gene="rpsM"
                     /locus_tag="IXF54_001636"
     CDS             19454..19819
                     /gene="rpsM"
                     /locus_tag="IXF54_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1404896.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILANAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19847..20236
                     /gene="rpsK"
                     /locus_tag="IXF54_001637"
     CDS             19847..20236
                     /gene="rpsK"
                     /locus_tag="IXF54_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1404897.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20316..21254
                     /locus_tag="IXF54_001638"
     CDS             20316..21254
                     /locus_tag="IXF54_001638"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1404898.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21282..21662
                     /gene="rplQ"
                     /locus_tag="IXF54_001639"
     CDS             21282..21662
                     /gene="rplQ"
                     /locus_tag="IXF54_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1404899.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21934..22773
                     /locus_tag="IXF54_001640"
     CDS             21934..22773
                     /locus_tag="IXF54_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1404900.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22749..23618
                     /locus_tag="IXF54_001641"
     CDS             22749..23618
                     /locus_tag="IXF54_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1404901.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23608..24405
                     /locus_tag="IXF54_001642"
     CDS             23608..24405
                     /locus_tag="IXF54_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1404902.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24608..25354
                     /gene="truA"
                     /locus_tag="IXF54_001643"
     CDS             24608..25354
                     /gene="truA"
                     /locus_tag="IXF54_001643"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1404903.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4713 c         5495 g         7028 t
ORIGIN      
        1 agttggcagg agaacaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgtactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catatttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aggacttctc cgttttcgtc
     1861 tgtacgcaag acaggttgga atcctttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatcccttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat tatggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact acactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg atcgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attacttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt ctcaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataatag aaggaacgcg aaataaaaga gtcagtattt
     7321 tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaatcctt gctttgtgag
     7381 tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca
     7441 gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa
     7501 cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga
     7561 acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag
     7621 atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc
     7681 caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca
     7741 aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc
     7801 caacaccaaa aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg
     7861 aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct
     7921 tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa
     7981 ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct
     8041 acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga
     8101 aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg
     8161 ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag
     8221 acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc
     8281 acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa
     8341 tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg
     8401 gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta
     8461 tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aatcactatc aaatcagctg
     8521 tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat
     8581 tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg
     8641 aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag
     8701 gaacacacgc ggttaaaaac cgtagcgctg ttcgtggcgg tggacgtaaa ccatggcgtc
     8761 aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg cgtggaggtg
     8821 gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc
     8881 gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg
     8941 aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat
     9001 ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag
     9061 cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg
     9121 tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg
     9181 cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc
     9241 catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa
     9301 acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg
     9361 tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat
     9421 cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact
     9481 aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc
     9541 tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct
     9601 gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc
     9661 actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt
     9721 aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct
     9781 aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga
     9841 ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct
     9901 cttccattag aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt
     9961 aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc
    10021 aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt
    10081 gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga
    10141 cgctctcgtt ggatgcgtaa acgcccgact gtacgtggta gcgtaatgaa ccctaatgat
    10201 cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct
    10261 tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt
    10321 attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg
    10381 aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa
    10441 aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt
    10501 tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat
    10561 gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca
    10621 agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg
    10681 ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac
    10741 ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc
    10801 aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat
    10861 gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag
    10921 cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc
    10981 agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg
    11041 agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg
    11101 actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa
    11161 gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg
    11221 aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg
    11281 gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa
    11341 gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg
    11401 aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca
    11461 tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa
    11521 acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt
    11581 tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag
    11641 ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag
    11701 ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat
    11761 gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt
    11821 tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta
    11881 catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa
    11941 agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt
    12001 aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc
    12061 gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat
    12121 gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg
    12181 atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc
    12241 aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg
    12301 tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa
    12361 gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta
    12421 ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta
    12481 agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta
    12541 tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag
    12601 cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga
    12661 ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac
    12721 gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg
    12781 atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg
    12841 tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata
    12901 ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac
    12961 gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac
    13021 tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt
    13081 ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg
    13141 ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta
    13201 aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc
    13261 caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct
    13321 acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata
    13381 aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta
    13441 aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta
    13501 aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact
    13561 tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag
    13621 ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta
    13681 cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac
    13741 gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt
    13801 taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac
    13861 gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt
    13921 tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa
    13981 aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt
    14041 gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct
    14101 tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag
    14161 taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt
    14221 tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc
    14281 aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga
    14341 atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg
    14401 cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa
    14461 agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga
    14521 aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta
    14581 cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata
    14641 aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta
    14701 aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag
    14761 gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa
    14821 caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt
    14881 tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg
    14941 ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag
    15001 ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa
    15061 acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg
    15121 aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga
    15181 agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac
    15241 ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa
    15301 aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga
    15361 catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt
    15421 cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca
    15481 agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc agcaaaagct
    15541 gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac
    15601 cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat
    15661 ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt
    15721 tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac
    15781 gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtaggatttg
    15841 gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga
    15901 aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt
    15961 ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg
    16021 gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag
    16081 gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac
    16141 gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag
    16201 gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca
    16261 aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa
    16321 gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga
    16381 agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac
    16441 ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta
    16501 cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag
    16561 gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa
    16621 aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga
    16681 accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga
    16741 aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg
    16801 tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa
    16861 tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc
    16921 gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt
    16981 accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt
    17041 gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc
    17101 atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt
    17161 tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct
    17221 gacattagta ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc
    17281 tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct
    17341 aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg
    17401 aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag
    17461 agaaatttat gaagattact ttataaatat tgatccttca gacatatgga aatctgctat
    17521 ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca
    17581 acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag
    17641 cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc
    17701 gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa
    17761 ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta
    17821 tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa
    17881 attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg
    17941 gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct
    18001 tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat
    18061 cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca
    18121 gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt
    18181 gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca
    18241 ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt
    18301 atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag
    18361 ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa
    18421 caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag
    18481 atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga
    18541 ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc
    18601 tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta
    18661 caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg
    18721 ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct
    18781 tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg
    18841 ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt
    18901 gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact
    18961 tctagagaat cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg
    19021 ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa
    19081 gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt
    19141 gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac
    19201 atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt
    19261 ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca
    19321 tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag
    19381 gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg
    19441 aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt
    19501 agtttcactt acttatatct atggtattgg taacactact gctaaaaaga tcttagctaa
    19561 tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat
    19621 ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa
    19681 catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc
    19741 tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat
    19801 cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa
    19861 agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc
    19921 tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc
    19981 atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat
    20041 ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac
    20101 agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt
    20161 agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa
    20221 acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca
    20281 agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa
    20341 tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag
    20401 ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg
    20461 ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg
    20521 ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg
    20581 gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg
    20641 atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct
    20701 ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag
    20761 cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca
    20821 cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt
    20881 ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga
    20941 gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag
    21001 caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa
    21061 tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa
    21121 tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact
    21181 taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac
    21241 gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag
    21301 gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca
    21361 acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa
    21421 tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac
    21481 gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac
    21541 aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca
    21601 ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct
    21661 aaaatcacac taataacatc actttataga tgattctttt agagtgttat gatgttggaa
    21721 gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag
    21781 cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa
    21841 ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt
    21901 tagtaggaac tttaggatga agaaggagta atcatggagc caattattga attagaaaag
    21961 ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca
    22021 attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt
    22081 gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa
    22141 atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt ttttcaaaat
    22201 ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat
    22261 caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga
    22321 atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt
    22381 gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt
    22441 atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa
    22501 agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg
    22561 atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct
    22621 gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc
    22681 gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg
    22741 gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat
    22801 ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat
    22861 tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta
    22921 agccaacaag cggtacagtc catattggag aacgagatat tcagccggat acggataata
    22981 agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc
    23041 tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg
    23101 aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct
    23161 atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg
    23221 gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc
    23281 cccagggtag aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca
    23341 ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg
    23401 tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg
    23461 aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa
    23521 tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta
    23581 tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg
    23641 cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat
    23701 tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc
    23761 acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc
    23821 ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga
    23881 gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc
    23941 tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca
    24001 ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg
    24061 ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg
    24121 atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa
    24181 ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc
    24241 agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg
    24301 gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt
    24361 gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg
    24421 ctatctattc ttttcttgct gatctgtttg caagaaagga tgcgagcagt gtatgacaag
    24481 aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa
    24541 gaacgaaaaa attttataaa aaaatcatta ttccagataa ccttttagga acaaaggaga
    24601 gaaaaacatg gtacgttaca aagcaatcat tgcatacgac ggaacaaatt ttaatggatt
    24661 ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat
    24721 ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc
    24781 gattgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt
    24841 cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga
    24901 agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg
    24961 gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc ctcttgattt
    25021 ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg
    25081 tgcatcagga agctcgatag aggataaagt acgtacgatc tacgaggcta agatggaggt
    25141 aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat
    25201 gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag
    25261 tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag cacatcctga
    25321 aggactttat ttgtatgaag tgaaatatga ataagaggtt gtgacagaag tggtcagctt
    25381 caagaaataa gaaggaattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta
    25441 ttttcgaagg agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc
    25501 atcaagtaat aagaacaacc aaacaaaaat agctc
//
LOCUS       DACERL010000040        24944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.041, whole genome shotgun sequence.
ACCESSION   DACERL010000040 DACERL010000000
VERSION     DACERL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24944
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.041"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(12..2156)
                     /locus_tag="IXF54_001644"
     CDS             complement(12..2156)
                     /locus_tag="IXF54_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1404904.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2513..3886)
                     /locus_tag="IXF54_001645"
     CDS             complement(2513..3886)
                     /locus_tag="IXF54_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404905.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IXF54_001646"
     CDS             complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IXF54_001646"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1404906.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IXF54_001647"
     CDS             complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IXF54_001647"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1404907.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5334..6347)
                     /locus_tag="IXF54_001648"
     CDS             complement(5334..6347)
                     /locus_tag="IXF54_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1404908.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6428..7027)
                     /locus_tag="IXF54_001649"
     CDS             complement(6428..7027)
                     /locus_tag="IXF54_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1404909.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7213..7914
                     /locus_tag="IXF54_001650"
     CDS             7213..7914
                     /locus_tag="IXF54_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404910.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7924..10635
                     /locus_tag="IXF54_001651"
     CDS             7924..10635
                     /locus_tag="IXF54_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1404911.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11086..12714
                     /gene="treC"
                     /locus_tag="IXF54_001652"
     CDS             11086..12714
                     /gene="treC"
                     /locus_tag="IXF54_001652"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1404912.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12774..13955)
                     /locus_tag="IXF54_001653"
     CDS             complement(12774..13955)
                     /locus_tag="IXF54_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1404913.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14207..15730
                     /locus_tag="IXF54_001654"
     CDS             14207..15730
                     /locus_tag="IXF54_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1404914.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15799..16527)
                     /locus_tag="IXF54_001655"
     CDS             complement(15799..16527)
                     /locus_tag="IXF54_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1404915.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16540..17820)
                     /locus_tag="IXF54_001656"
     CDS             complement(16540..17820)
                     /locus_tag="IXF54_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1404916.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17961..18563)
                     /locus_tag="IXF54_001657"
     CDS             complement(17961..18563)
                     /locus_tag="IXF54_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1404917.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18808..19014
                     /locus_tag="IXF54_001658"
     CDS             18808..19014
                     /locus_tag="IXF54_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404918.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19326..20045
                     /locus_tag="IXF54_001659"
     CDS             19326..20045
                     /locus_tag="IXF54_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1404919.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20126..20809)
                     /locus_tag="IXF54_001660"
     CDS             complement(20126..20809)
                     /locus_tag="IXF54_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1404920.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21027..23213
                     /locus_tag="IXF54_001661"
     CDS             21027..23213
                     /locus_tag="IXF54_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1404921.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23559..23969
                     /locus_tag="IXF54_001662"
     CDS             23559..23969
                     /locus_tag="IXF54_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1404922.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            23983..24339
                     /locus_tag="IXF54_001663"
     CDS             23983..24339
                     /locus_tag="IXF54_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1404923.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8358 a         4430 c         4864 g         7292 t
ORIGIN      
        1 tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac
       61 aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt
      121 cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg
      181 ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc
      241 catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca
      301 gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc
      361 gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt
      421 gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca
      481 gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat
      541 ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg
      601 aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg
      661 cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat
      721 gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaat
      781 tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat
      841 gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca
      901 aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca
      961 tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt
     1021 ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat
     1081 tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa
     1141 gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca
     1201 agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat
     1261 acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata
     1321 ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa
     1381 ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg
     1441 tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt
     1501 gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt
     1561 gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct
     1621 ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa
     1681 tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta
     1741 atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata
     1801 tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc
     1861 aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca
     1921 gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg
     1981 tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt
     2041 gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca
     2101 gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata
     2161 aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt
     2221 ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa
     2281 taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat
     2341 tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt
     2401 aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag
     2461 aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat
     2521 aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac
     2581 taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg
     2641 tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac
     2701 cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt
     2761 aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg
     2821 aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa
     2881 aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga
     2941 tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc caaccaccag
     3001 cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta
     3061 aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga
     3121 taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga
     3181 ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg
     3241 tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg
     3301 aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc
     3361 cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga
     3421 cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa
     3481 tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac agcggaatga
     3541 tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt
     3601 tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat
     3661 ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct
     3721 gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg
     3781 cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa
     3841 tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc
     3901 actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca
     3961 gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa
     4021 caataatttg attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc
     4081 aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag
     4141 accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc
     4201 cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt
     4261 gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga
     4321 ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac
     4381 agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag
     4441 tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac
     4501 tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc
     4561 tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa
     4621 actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat
     4681 aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt
     4741 caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa
     4801 gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag
     4861 gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc
     4921 tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc
     4981 agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc
     5041 cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt
     5101 ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt
     5161 gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt
     5221 gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa
     5281 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
     5341 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
     5401 ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag
     5461 attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
     5521 tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat
     5581 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
     5641 tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
     5701 tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
     5761 ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag
     5821 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
     5881 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
     5941 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
     6001 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
     6061 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
     6121 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
     6181 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
     6241 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
     6301 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
     6361 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
     6421 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
     6481 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
     6541 aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat
     6601 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
     6661 gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca
     6721 tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag
     6781 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
     6841 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
     6901 ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt
     6961 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
     7021 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
     7081 tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa
     7141 ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat
     7201 ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga
     7261 gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg
     7321 attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg
     7381 gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat
     7441 aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc
     7501 gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg
     7561 cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg
     7621 tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat
     7681 cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa
     7741 gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca
     7801 cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta
     7861 aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat
     7921 tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga
     7981 cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa
     8041 actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct
     8101 gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag
     8161 atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac
     8221 gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact
     8281 gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa
     8341 gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat
     8401 accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg
     8461 aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca
     8521 gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc
     8581 aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat
     8641 cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca
     8701 cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat
     8761 gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga
     8821 acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa
     8881 ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa
     8941 aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt
     9001 tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt
     9061 ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa
     9121 aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa
     9181 aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta
     9241 ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg
     9301 agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt
     9361 gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca
     9421 ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg
     9481 ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag
     9541 tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc
     9601 gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac
     9661 tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat
     9721 atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa
     9781 ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct
     9841 gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa
     9901 acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat
     9961 atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa
    10021 aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa
    10081 tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa
    10141 atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa
    10201 caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc
    10261 gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc
    10321 gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg
    10381 atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt
    10441 gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt
    10501 ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg
    10561 gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg
    10621 aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa
    10681 acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc
    10741 tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat
    10801 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
    10861 agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
    10921 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
    10981 aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata
    11041 taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
    11101 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
    11161 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
    11221 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
    11281 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
    11341 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
    11401 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
    11461 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
    11521 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
    11581 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
    11641 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa
    11701 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
    11761 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
    11821 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
    11881 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
    11941 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
    12001 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
    12061 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
    12121 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
    12181 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
    12241 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
    12301 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
    12361 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
    12421 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    12481 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    12541 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc
    12601 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    12661 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    12721 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    12781 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    12841 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    12901 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    12961 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    13021 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    13081 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    13141 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    13201 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    13261 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    13321 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa
    13381 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    13441 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    13501 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa
    13561 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    13621 tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa
    13681 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    13741 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    13801 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    13861 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    13921 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    13981 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    14041 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    14101 aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg
    14161 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    14221 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa
    14281 agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt
    14341 tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga
    14401 ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt
    14461 tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga
    14521 aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga
    14581 aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca
    14641 tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat
    14701 tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa
    14761 acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt
    14821 tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc
    14881 gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca
    14941 ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat
    15001 tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg
    15061 attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct
    15121 cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa
    15181 aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct
    15241 ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga
    15301 tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga
    15361 atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat
    15421 ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga
    15481 aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa
    15541 ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc
    15601 cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga
    15661 gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct
    15721 tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc
    15781 caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat
    15841 ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg
    15901 tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc
    15961 tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg
    16021 cgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc
    16081 attaaactct attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat
    16141 tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata
    16201 atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac
    16261 atagattatt tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc
    16321 catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat
    16381 cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa
    16441 gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc
    16501 gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt gtatataaat
    16561 cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt
    16621 tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga
    16681 gaaccctttt tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg
    16741 aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc
    16801 actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt
    16861 atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc
    16921 ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc
    16981 caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg
    17041 atcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa
    17101 aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc
    17161 tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt
    17221 attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa
    17281 aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat
    17341 aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac
    17401 agtgtgaaac caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa
    17461 agcggataag acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt
    17521 caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc
    17581 aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat
    17641 cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat
    17701 caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc
    17761 aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat
    17821 aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta
    17881 atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt
    17941 cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc
    18001 atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa
    18061 taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg
    18121 ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta
    18181 cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt
    18241 gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt
    18301 agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg
    18361 atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa
    18421 aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt
    18481 acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat
    18541 ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa
    18601 aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct
    18661 cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa
    18721 aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac
    18781 cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa
    18841 acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc
    18901 tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct
    18961 cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag
    19021 ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga
    19081 ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg
    19141 caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat
    19201 tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa ggagcagata
    19261 aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag
    19321 gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac
    19381 taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata
    19441 atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt
    19501 tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac
    19561 tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg
    19621 tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag
    19681 aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa
    19741 ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg
    19801 aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg
    19861 atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat
    19921 gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag
    19981 atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta
    20041 aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt
    20101 gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc
    20161 ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg
    20221 aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa
    20281 gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat
    20341 aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg
    20401 cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa
    20461 tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg
    20521 cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa
    20581 ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac
    20641 caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt
    20701 gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct cctccgataa
    20761 atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca
    20821 ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa
    20881 ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta
    20941 taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga
    21001 agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag
    21061 aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag
    21121 aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag
    21181 tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct
    21241 ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc
    21301 gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc
    21361 tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg
    21421 ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata
    21481 tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg
    21541 aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg
    21601 ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa
    21661 aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg
    21721 ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag
    21781 aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag
    21841 agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt
    21901 atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc
    21961 aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag
    22021 atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg
    22081 aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta
    22141 ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga
    22201 tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag
    22261 cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata
    22321 aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc
    22381 tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta
    22441 tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc
    22501 aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga
    22561 aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc gttgcagatt
    22621 atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg
    22681 aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc
    22741 atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag
    22801 taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag
    22861 ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta
    22921 tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta
    22981 acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa
    23041 taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga
    23101 ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga
    23161 aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga
    23221 gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga aaattgcttc
    23281 tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa cacagtttat
    23341 tcggaaaagg gaggtgtgac aagaattttg tcacacctcc cttatttatt ttgaaaatga
    23401 gaatactatt atcaaaatag agaatatata gaaaaattct cattattcaa acgtaaataa
    23461 tcagttattt tgattttttt tatattttat tagctaataa ttggtatttt aaaagaaatt
    23521 actgtagaat tggattataa ttaaaaagga gtgagaaaat gataaaagta tatacttcgc
    23581 ctagctgtac ttcctgtaga aaagcaagag catggttgca agcgaatcaa cttgaatttg
    23641 aagagaagaa tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag atcttggcag
    23701 cgactgaagg aggagttcaa gatattattt ccactcggtc gaaaatttac gaaaagctgg
    23761 gcatagattt taacgaatta actttaaagc aaatgatcgt cttattcaaa gaatatcctt
    23821 cattattgag acgacctatt ttgattgatg aaaaaagaat actaatcgga tttaatgaag
    23881 atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt
    23941 tacaaaacaa taacatctta aattcatagg gagggagcct tcgtggaagt gaaagtctat
    24001 ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat ggttagaaca ccatcaattg
    24061 ccttatcaat ttgttgactt agaagaacgc gatctaagta cagaagaagc agaagaatta
    24121 tgcagttttg aagaagtgca agcagaacag ctatttgcga cttggtctga ggcttttcac
    24181 aacattgcga ttgacctttc ctgtgtagga aaaaaacaac tgattttttt atgcacacaa
    24241 cataccaatc tcctcagacg tcctttgatt attatcgatg aagttctatt tattggatac
    24301 aatgagcagt tgatggaaca gcttatctta aataaataga ctttaactgt tttaatcaag
    24361 tcactgagca tttcatacag ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt
    24421 tctttttttg actttatttt tctactatag agacatgctc aatttatgtg gtcaaaattc
    24481 aaatctaggt ggggatatag agttaaaaat atattgacat gtgtaaattt acatgatata
    24541 ttcatttctg ttgttttaag tagcaaagca ataaaatagt attttaaaaa acatataaaa
    24601 tagttgttga catgttagaa atgacatgat aagataactg agttgttaaa aaagaaccac
    24661 ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa taacaaatga
    24721 taagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa aaacttttaa
    24781 aaaagagttg acaattactt gtctctctga tatgatataa aagttgctac ggcagccaaa
    24841 aacgaatcgg ggaacactcc tgatgaagta gacctttgaa aactgaacaa agtaagacaa
    24901 accaaatgtg tagggcgtct tgattcaatt caagacaaca aaca
//
LOCUS       DACERL010000041        24746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.042, whole genome shotgun sequence.
ACCESSION   DACERL010000041 DACERL010000000
VERSION     DACERL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24746
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.042"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..585
                     /locus_tag="IXF54_001664"
     CDS             <1..585
                     /locus_tag="IXF54_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404924.1"
                     /translation="DRLKKQESLKKINDQSDESTNLTALRNQNRRTGQRSKKRETGHQ
                     TLMKRQGEKVLQPINKPKDMKKQVVARSQRRTGQPTTRQNIQPKVAAQMVKRSGGTLG
                     SNPAYRRPEVRKKMDQVKQVTQVDVPQTHSTPQRVQPKVNEPITRMNKHNTPIIKKEK
                     RTKQSPVVRRERKKPGTQKITNKPKGVRQPRARK"
     gene            572..2830
                     /locus_tag="IXF54_001665"
     CDS             572..2830
                     /locus_tag="IXF54_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ1404925.1"
                     /translation="MQGNNLFNNGLLQPKKPPIGQEQQKMTRYHQLRLVFGLVGVLLY
                     PFAILGSIPLLIGLAVDKRDKAANVFDMDYESFLKRNSIVFLSFSAVLFVINVFAFIL
                     WIPRGYLSAYLLFPLNLLHTALRFNWETIVALLIGSSGMGAIFLAFSSFVAKRKVISK
                     EDERKKITESKAYKDREKNKFEESQRFTDEQEEAYEEAVETVDIDKYKELSNQLLLGT
                     SEFGLPYIINFSEFNQHVLVPATTGSGKTTLLQLLVQHAVKFNFPVILIDGKGARDTL
                     ESMREIAKFYDKEVHAFTDDGDMRYNPVEHGNDISIRDKLVSLAETESVFYSGAAKAL
                     LQVTIQLLDEFKGAKVTLSGDTRTTETVERSLPFVQRFLLPRNVLHLFADAILPNNPK
                     LFEIEVEKKIQKPKKKSVKEGSEILPDSDLDKEEKEEDSQIKNSKFRNISQLGIAQKE
                     TETIVLNPETLDLDSYYLLLKRNLRYLPTDKETGENIKQKLFERLFIRYEHKDSPFYL
                     YATSEALQTNINMLLDSELGKLFDTKNAKNVLDVQEIVNQRKLVYVSFNGLIYKEYIR
                     TLAQMLVGDVNYFASEMYRKNVKREVLVIFDEPASYLNETFIDMVNKGRGAGVYGIFT
                     PQTMADIAKLGDKLMEQLVGNVNTLFIGKTNEKGEAEYWSETMGTYQDIDVTSVTEQE
                     DGYSDVGKSDWSGDRGTKRNVDRFKISPNKIKELRTGEFIIYRTAENVNLPPQKVYVR
                     NALNWLKNNSKI"
     gene            3352..4137
                     /locus_tag="IXF54_001666"
     CDS             3352..4137
                     /locus_tag="IXF54_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1404926.1"
                     /translation="MKPLLYNYYIDYTKKDKTRLIILCLLHELRVISVQQLIDFFQIE
                     RLSAESTVYKHLNLLKTDGLIARSKNGMHTFYYLTKEGHNYIGGYYTLPKVPEYNLQH
                     HLQINDYLIKMLELCNKHPHLKAVVSERRKVYEVKDEKKNQKGVKYFVPDFIFMFLDS
                     IGREVEWQFEIELTLKTKRRYSQGVFPKYIKHLKNYEDARLIYVTPSSIIKEELDMFK
                     EYFIDKEGDEYKEVFDRLHVFSAEEFESELKRLLEKDKFINWE"
     gene            4143..4631
                     /locus_tag="IXF54_001667"
     CDS             4143..4631
                     /locus_tag="IXF54_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404927.1"
                     /translation="MRRIEKEFNKKLAGYERELKKLGCLDDETGLIPISKRRWHVIWW
                     QPVTLAKTIVRSYRLTLDNQNLCILGDVEITIYHDGTYGISKEAVPIFINDLLSLKKL
                     ITIFYGTPFNLNFEKIRCVNFNRYCVSIPEIYVEKFEVLINYLIILSSCLHEVKKHVE
                     YD"
     gene            5223..5489
                     /locus_tag="IXF54_001668"
     CDS             5223..5489
                     /locus_tag="IXF54_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404928.1"
                     /translation="MLTTKEKNRFKKMVEGNKTFHYSYVDRLRQDVRYYVNQCESAVK
                     ARESMEILEFIYSLFSDKELPAWYTKADLENDKKSIEKLERWAA"
     gene            5522..6016
                     /locus_tag="IXF54_001669"
     CDS             5522..6016
                     /locus_tag="IXF54_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HAQ1404929.1"
                     /translation="MKFIKDVTTLEELKRVYKKLALKYHPDMGGTDKEMAQINNEYEA
                     LFDKLKNTHKNKEGEYYQKETTETPQEWQEIIHKLLTLKMENVSIEVIGAFLWVSGNT
                     KPYKEELKALNMKWSNNKKSWYLAPDGYKKRSKKKYDMSDIRNMYGSQVVKEEQVKQK
                     TLTA"
     gene            6114..6530
                     /locus_tag="IXF54_001670"
     CDS             6114..6530
                     /locus_tag="IXF54_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010710134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1404930.1"
                     /translation="MIKTSAVGRLTKSSELRVTSTGKAVANFALACKRERLDKDGNAQ
                     TTFIQCVIWGKPAEVLAKYTQKGSLIAVNGELQSRSYDDQQGQTHYVTELVVAGFEFL
                     ESKETVQNREQKQQMPEMANAEPFYGMDDMEPPAYH"
     gene            6554..7138
                     /locus_tag="IXF54_001671"
     CDS             6554..7138
                     /locus_tag="IXF54_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAQ1404931.1"
                     /translation="MKKLIGKKWLLLTAVATFLLSGCASLEQKAQDSVKEVTENVTQT
                     ISNDQRIPADFVRHVDGDTTVLKIDGKEQKVRFLLIDTPETVKPKTKVQPFGLEASKR
                     TKELLSNASEITFEYDQGDKTDRYGRALGYIFVDGTLLQKTLVSEGLARVAYIKEPNT
                     KYLSELEQAQEQAKNESLGIWSIPGYVTQRGFSK"
     gene            7249..7515
                     /locus_tag="IXF54_001672"
     CDS             7249..7515
                     /locus_tag="IXF54_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1404932.1"
                     /translation="MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSI
                     NEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVEDLMEDLNA"
     gene            7508..7783
                     /locus_tag="IXF54_001673"
     CDS             7508..7783
                     /locus_tag="IXF54_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HAQ1404933.1"
                     /translation="MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKY
                     KDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTLARIGSHSELFR"
     gene            8586..8771
                     /locus_tag="IXF54_001674"
     CDS             8586..8771
                     /locus_tag="IXF54_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404934.1"
                     /translation="MNELQLKLDLEKAQLEYQKLSQAINENDTVTLLLNYGCLKNAND
                     RLNQLSFLLNHIEWKDV"
     gene            8793..8939
                     /locus_tag="IXF54_001675"
     CDS             8793..8939
                     /locus_tag="IXF54_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfM protein"
                     /protein_id="HAQ1404935.1"
                     /translation="MTIVETLEVAVYVYAESEEQAHELVSNMYYDQEIILTEENHETT
                     EFII"
     gene            9170..9949
                     /locus_tag="IXF54_001676"
     CDS             9170..9949
                     /locus_tag="IXF54_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404936.1"
                     /translation="MDFQNIQTVKELTHALKQYNQNLATTIFDDTQGYISVAFKKDKI
                     EKNGNDFQFLCFYPNYEVALYPVVTLLSYLEKLPEQATVAIKDPDGNYSSLYLTIDET
                     IDKGTKYKWLVISDRLSYERFYLTSQLTIQEQALEKMLKELETEPNNRLEAIHDWLCQ
                     QTDKDLLVGILKENRTLKGAEAYCTQKAYEIRNGNGAMASHITVFNWVREYYTLDELP
                     TDKPKIQATIKSDYINQKIQRPTPVETKNNNEIDLFEGEDF"
     gene            9949..11208
                     /locus_tag="IXF54_001677"
     CDS             9949..11208
                     /locus_tag="IXF54_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404937.1"
                     /translation="MNTTDYVENKLQPPRAFFEWCYSSFRTYVWKNKNETIVSSTRKH
                     DWIIEKKLRKNSRLTFYDSSSCFQIILSTSKRIEVQTYKVISEYENGVQCFREQLECI
                     EIFSNNQHIKIGKICLPVYYGYNMGIALYPNEWKKRLERVSELKYLNLERLNVDNLAT
                     TYKYRTLIEFAQKINAHKLAYDVMSGAVDMRILTKNCLRKYKTFLKNTDNSLKEIQLK
                     QTFESLNIPMVKGIEKYVLKSDISDFPNEIGAVKFQNWLVKQGKSFKYYQDYLNMLTL
                     LKIEINKRNQLPPDLEVAHDCAVDRINQLNYEKRDKEINERLKQLRKYERDIDGYTFV
                     LPKRANDIKKEGKALNHCVASYISRHAKGETTIIFVREKKNPQKSYFTLEYNYNRVVQ
                     LQGKKNRQKVPDELKQAVDKWVKVIKD"
     gene            11858..12586
                     /locus_tag="IXF54_001678"
     CDS             11858..12586
                     /locus_tag="IXF54_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404938.1"
                     /translation="MTRLEQAQGKLQRLKRESEEMHHLIRAEHDRIPFGQPNIIGRGD
                     IYKKVNGYHDRAIKLLKEQEKQEKRVEMLEKVEDFKEKNELIKDVHVVGKSSYATVGA
                     KTSVNNIDYFKNELKELEKANEKAKAYNKTKPAIKARTFGAVITKLKNKIATLEQMKE
                     ADENKVVSERTKELIESGAVTQWKKKPIFYFVKGLRKVALEIDENGEFFISNYYPACT
                     DADKEFISKLLDPAAESRKKETFC"
     gene            12797..13207
                     /locus_tag="IXF54_001679"
     CDS             12797..13207
                     /locus_tag="IXF54_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404939.1"
                     /translation="MANETLKKEAWYRMMLTDLSSSVIDEFMETGKCHYTSNYFQGEN
                     ILVTEEIEAIIKTVEKKYGFLVYYVTENKTADGQRFLSLFYVGRDTSDWLYCHRDLES
                     YRQYVYVVNTTNPAFSEFGMIQFDPILGSLLRTA"
     gene            13293..13631
                     /locus_tag="IXF54_001680"
     CDS             13293..13631
                     /locus_tag="IXF54_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404940.1"
                     /translation="MSMEIRELIIIEDPCFYQYTTIKELESFNTSELTIALSVLRKEQ
                     RRLKEKVYHSGSGDQVDKNNVKLINKYRANLEKLLFIKQGYIPDAITQNMIDTLIKNN
                     KKSIQKIQKG"
     gene            13846..14505
                     /locus_tag="IXF54_001681"
     CDS             13846..14505
                     /locus_tag="IXF54_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ1404941.1"
                     /translation="MDEKRKDKIQRLLALANDANDEESMTALAKAQELMLAYGIDEEE
                     VFHYQQHQRQEKVIDKVIYKGRPHKWVYRLAGIIAKNFRVKNYYQSSSDIELHYVGLT
                     TDVEIAEITFLYAKGSVAFCSKEFMKQPEIKRKYKRKWQLKQDYIEGYLTALSSVFEA
                     QVLTNGYELALQLPVAVHEEVSKLGLVKGKDTSHHVENFDAFHSGYQEGLQFKSKELI
                     N"
     gene            14634..14792
                     /locus_tag="IXF54_001682"
     CDS             14634..14792
                     /locus_tag="IXF54_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404942.1"
                     /translation="MDVLNLMHAQGVVIGLKKTYAVNVYRKRTKKADKKLFAFFTYIF
                     YTIKKRGF"
     gene            14896..16356
                     /locus_tag="IXF54_001683"
     CDS             14896..16356
                     /locus_tag="IXF54_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404943.1"
                     /translation="MNKFQSWLMANKNLLITSVVMCQVLVLSIGIINFPYIDDTGRQI
                     DGNTDFARSYSRWGSEIGSWLVQGSRHLTDMGLWTHILSGFIVSVTGIIIVYSFHKKL
                     SLLALTAAMLVGFNPWFLQNLSFRFDSPYMSLSLLFSVLPFLFWNKNKVLFLVVSILS
                     IFLMCNTYQASSGIYVVMVLALSLKQLLDNQSFIAVLKKAIVAMISYISAMFLYLIET
                     KFNPEIATRGGMTTIASVKDIPKTILVNSQMYLSKITEQSTKLWILLFFILVIFFVLS
                     TVLNAKVSPVKSFLYAVLFLFLGSILSYGVFLIFPEKLLLISPRYGYGFSFFVVSIIM
                     LTLSNTSKNVLGYVSKTAVCLFCVYLLSFTFVYASALSHQKESFERQSMILADDLKDL
                     VNRDTVTVHSTSLFKDSPVFANSSKNYPILKELVPPNEALYWPNQFLFRTYTGLNVNM
                     EIFDINALNKEESELMKSNYYHDIYVKDSEVFVHVK"
     gene            16721..17110
                     /locus_tag="IXF54_001684"
     CDS             16721..17110
                     /locus_tag="IXF54_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ1404944.1"
                     /translation="MGDIDLENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYK
                     KSLIENNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLL
                     PLVGIITTGICLFKKGKDNKTDSSQVS"
     gene            17609..18268
                     /locus_tag="IXF54_001685"
     CDS             17609..18268
                     /locus_tag="IXF54_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HAQ1404945.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
     gene            complement(18527..18871)
                     /locus_tag="IXF54_001686"
     CDS             complement(18527..18871)
                     /locus_tag="IXF54_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404946.1"
                     /translation="MLEGGIQMKKIVWIYSVNLKGMGLYGNSTTMPLRQAQKFQETIK
                     TNLPSDVTVDFISYDTSSTEIPKADLIVYNDIDSRYLSDDLKNNGIVIPFKDMISNNT
                     REIEKKILLAIK"
     gene            19086..19280
                     /locus_tag="IXF54_001687"
     CDS             19086..19280
                     /locus_tag="IXF54_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404947.1"
                     /translation="MSKLLRELTDKEIQSSLSNGGSTCSMYKALLPGHIAACAASAYF
                     YGSSGCTAMRYVSEYIRKNC"
     gene            19359..20504
                     /locus_tag="IXF54_001688"
     CDS             19359..20504
                     /locus_tag="IXF54_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1404948.1"
                     /translation="MLRNFSVIIICKNEERVIKRCIESIQKNLKKNDEIIVVDTGSTD
                     NTIKIISSIENVKLFNYEWKDDFADARNFGISKAKKDWIFFIDSDEIMSKGCLEQLNA
                     SINEAMAYSENNEKLVFSPKAVNTDDSVFYNAGRIFANDGNIKYEGCVHEYPVVTDKS
                     LTLVSLKLPRVIVRHDGYEKEVRADKNKTVRNITLIKKILQEEPNNSQYYYYYYRDSK
                     PLITIEEYEQGMLNFFEKFPDSPYIDQVVRDLAYHYIQVGKNELAEEYINMLFESAEN
                     GAKENYHLAILYTGMNEMGKIKAQQKDILKLLVYAHDNVLQQEEELFDKGYIFDDLIS
                     ILFFQIEEYETAYKICEKLDSCGYASNISKMISKLKVVLSPNSKNFR"
     gene            20596..22659
                     /locus_tag="IXF54_001689"
     CDS             20596..22659
                     /locus_tag="IXF54_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404949.1"
                     /translation="MKYKVIPQIGEEDCGAACVAMIVENYFKKKISICEIRPLIKNTK
                     MGTSFFDLKRGLKRLGVDCKISQAVKQKDVFEEITYPFITQIYNETGIHYITVFEKKR
                     NKLIVGDSSKNRQLSISISKFLKKWIPYVLEMDFKRSNIVYQIEQKAKEISIWKIMNS
                     LKLQLFLSLCLSIIVYVASVIIANIYTLYFNILIPQNLGELALQLMFIYILINFFSFS
                     LTFINNFIYNSMSKKIDEKIIGKYFNGLLNKPNMAIESYEIGELLTNLSNVILIRQRF
                     LTYLQLLPISVLTITGSFYLLYLSDEKLSFFVILLVIVLSLIIYLSDSHYEKLSKELI
                     ETGQDFNDSVIDTFTNVSIIKQLGLEKEFGNRGKKKLTNYIISRTKMLNFDSILNQIK
                     SFVLSSFNIILFSTGVYLITLNQLSSGVLLTFNALLAYVTNPILNLANLQSVLVQGKV
                     AQEKLYNILESQIVLYGNKSLVIENDDIFINFDLVSYDYDSNSKILKDISINICGNNI
                     ALSGTNGVGKSTIGKLIARLYIPDSGSIEINGQNLIDISEESVSKNIIYVDGRESLFS
                     SSVSDNIKLGRNIKDKDIYDTLSKLKANSIFQNLDFENISDKQLSLGQMQIIKVLRAT
                     LSAKNIYIFDEITNGLDECVKQNVVDYLFGLKGMKIFITHDKEVISKCEKEYTVKDMK
                     IVRRR"
     gene            22661..23071
                     /locus_tag="IXF54_001690"
     CDS             22661..23071
                     /locus_tag="IXF54_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1404950.1"
                     /translation="MKKIFISISILVITGILGMLMYNNITSEKTYYTETKFGKISELV
                     KKNDRIILFVEQDGCSSCKLVKPIINNYAKENKGIVYSVTANKEKDYNVQSEKYHIEG
                     TPTLIYFKEGKEVYRTNTGFTEDEFQNTIKKVEF"
     gene            23073..23597
                     /locus_tag="IXF54_001691"
     CDS             23073..23597
                     /locus_tag="IXF54_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1404951.1"
                     /translation="MNKKEYLGIILIALASISIILFLYSNNRELKKANTMLQLEKQVL
                     IDNTDSFEYLNSITVDTFENRIKNKEDIFVYIGNSECADCSTFSKNLKNEVNNFPLKN
                     SLYLVEISRLHRNKKKWTEFKLKYGFDQTPAFILFKNGKVNSMIQWDEKKGLSSDSFH
                     RWLEENRNDIENLS"
     gene            24065..24682
                     /locus_tag="IXF54_001692"
     CDS             24065..24682
                     /locus_tag="IXF54_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1404952.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNAIQQKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKSKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRKRYNVTVDQRKNKGKRDS"
BASE COUNT         9289 a         3637 c         4613 g         7207 t
ORIGIN      
        1 gatcgcttga agaaacagga atcactcaaa aaaattaatg atcaaagcga cgaatcaacc
       61 aatttaacag ctttaagaaa tcaaaatcgg cgtactggac agcgaagtaa aaaacgcgaa
      121 acaggtcatc aaacacttat gaaacgacaa ggagaaaaag tacttcaacc aataaataaa
      181 ccaaaagata tgaaaaaaca agtggttgct cgtagtcaaa gacgaacagg acaaccaacg
      241 actcgtcaaa atatccaacc aaaagtagcg gcccaaatgg tcaaacgaag tggtggaacg
      301 ttgggaagta atcctgccta tcgacgtcca gaagtacgta aaaaaatgga tcaagtgaag
      361 caagtaacgc aagttgatgt acctcaaacg cattctacac cacaacgtgt acaacccaag
      421 gtaaatgagc caatcacacg tatgaataaa cataatacgc caataattaa gaaagaaaag
      481 agaactaaac aatcaccagt tgttcgcaga gaacgcaaga aacctggtac acagaagata
      541 acaaacaaac ctaaaggagt gagacaacct cgtgcaagga aataatttgt ttaacaatgg
      601 cttgctacag ccaaagaaac cgccaatagg gcaagaacaa caaaaaatga ctcgctacca
      661 ccaactccga ctagtctttg gattagtcgg agttctttta tatccttttg cgattttagg
      721 cagtattcca ttactaattg gtctagcggt tgataaaaga gacaaggctg caaatgtatt
      781 tgatatggat tatgagagtt ttttgaaacg aaatagtatt gtctttctta gtttttcagc
      841 tgtgttattt gtaataaatg tttttgcatt tatcctctgg attcccagag ggtatctgtc
      901 tgcgtatctt cttttcccct taaatcttct tcatacagca ctgcgtttta attgggagac
      961 aatagtcgct ttacttattg ggtcaagtgg aatgggagcc atttttttgg cattttcttc
     1021 atttgtggca aaacgaaaag tgatttcaaa ggaagacgaa cggaagaaaa taaccgaatc
     1081 aaaagcctac aaagaccgag aaaaaaataa atttgaagag tcgcaacgct tcacagatga
     1141 acaagaagaa gcgtatgaag aagcagtaga aacggttgat attgacaagt ataaagaact
     1201 atccaatcaa ttattgcttg gtacaagtga gttcgggttg ccgtatatca tcaacttttc
     1261 agagttcaat cagcatgttc ttgttcccgc aacgacaggt tcagggaaaa caacgttact
     1321 tcaattactt gtccaacacg cagtcaaatt taattttccg gtgatattga ttgatggaaa
     1381 aggcgctcga gatacattag aaagtatgcg agaaattgcc aaattttatg acaaagaagt
     1441 gcatgctttt acggatgatg gcgatatgag atacaaccca gtagagcatg gaaatgacat
     1501 atctattcga gataagttag tgtctttagc cgaaacagaa agtgtctttt attcaggcgc
     1561 agcgaaagct cttttgcaag taacaattca attgttagac gagtttaaag gagccaaggt
     1621 tactttatct ggagacactc gtacgacaga aaccgttgaa cgaagtctac catttgttca
     1681 acgattttta cttccgagaa atgttttaca cttatttgcg gatgcgattt taccaaacaa
     1741 tcccaaacta tttgagattg aagtagaaaa gaaaatacaa aaacctaaga aaaaatcagt
     1801 gaaagaagga agcgagatac tgcctgattc agaccttgat aaagaagaaa aagaggaaga
     1861 ttcacaaatt aagaacagta aatttagaaa catttcccaa ctaggaatag cacaaaaaga
     1921 aacggaaacg attgtcctta atccagaaac attagattta gactcgtact atttattact
     1981 aaaacgaaac ctaagatatt tgccaacaga caaagaaaca ggagaaaata tcaaacaaaa
     2041 attgttcgaa cgattattta ttcgttatga acataaagat tcaccgtttt atttgtatgc
     2101 cacatcagaa gcattacaga caaacattaa catgttattg gacagtgagt taggaaaatt
     2161 atttgatact aaaaacgcaa aaaatgttct cgatgtgcaa gaaattgtca atcaacgaaa
     2221 gttagtttac gtatcgttca atggattgat ttacaaagaa tatatccgca ctttagcgca
     2281 aatgttagtc ggagacgtaa attattttgc ttctgaaatg tatcgaaaaa atgtgaaacg
     2341 tgaagtcctt gtgatttttg atgaacctgc ttcttatcta aatgaaacgt ttattgatat
     2401 ggtaaataaa ggtagaggtg caggtgttta cggtattttc acccctcaaa cgatggcaga
     2461 tattgcaaaa ttaggcgata aattaatgga acaacttgtc ggaaatgtta atactttgtt
     2521 cattggaaag accaacgaaa aaggtgaagc cgagtattgg agtgaaacaa tgggaacgta
     2581 ccaagacatt gatgtaactt cagttacgga acaagaagat ggctattcag atgttggaaa
     2641 atctgattgg tcaggagata gaggaaccaa acgtaatgta gatcgcttca aaattagtcc
     2701 aaacaagata aaagaattgc gtacagggga gtttattatt tatcgtacag cagaaaatgt
     2761 aaaccttcca ccacagaaag tttacgtgcg aaacgcatta aattggctta aaaataatag
     2821 taagatttag aagaaatagc aaatgaaaaa cgagagtagc catagagtaa gcgtagagta
     2881 agtatagagt aggtatagaa taaaaaatct aggagagcgg tcctcggcgc ttggtataaa
     2941 aggctttctc cgcctgcaaa attcctgacc attttcgctc aacgtgggtt aaaaccccca
     3001 ttaagcgaaa atggtcagga attttgcaac tacgaaaaaa ataccaagcg ccgaggacaa
     3061 acagaataga gtaaaaaaat agaatgagta aggtgataga agtaataaaa attaaagaaa
     3121 aaagttcaaa agataaagtt caaaagatta acaactaccc cttgcgatag acgccccttc
     3181 accaaacctt gaagggacta tcgcccaaag caacaacctc ctaaatcctc caccaaaact
     3241 tggaggactt aaaagctgaa aaagaatttc tacacgttgt tccgaaactc tttttcaatg
     3301 aaacaaatag gctttgccta tttgtacttt ttatagaaat gcggtgatat tttgaaacca
     3361 ttactatata actattacat tgattacact aagaaagata aaacaaggct aattatttta
     3421 tgtctactcc atgaattaag agttatctca gttcaacaat taattgactt tttccaaatt
     3481 gaacggttat cagcagaaag cacagtttat aagcacttaa atttattaaa aacagatgga
     3541 ttaattgccc gatcaaaaaa tggaatgcac acgttctatt acctaacaaa agaagggcat
     3601 aattacattg gtgggtacta caccttgcca aaagttcctg aatacaattt acaacaccac
     3661 ttacaaatta atgattatct gataaaaatg ttggaacttt gtaataagca cccgcacctt
     3721 aaggcggtag tatcagaacg tcgaaaagta tatgaagtaa aggacgagaa gaaaaatcaa
     3781 aaaggcgtaa aatactttgt tcctgacttc atttttatgt ttttggatag tattggacga
     3841 gaagtagagt ggcaatttga aatagaatta acactaaaaa caaaacgacg ttatagtcag
     3901 ggtgtgtttc cgaaatacat taaacattta aaaaattatg aagatgcacg cttaatttat
     3961 gtaacaccta gttcaatcat taaagaagaa cttgatatgt ttaaagaata ttttattgac
     4021 aaagaagggg acgagtacaa agaagtgttt gatcgattgc atgttttttc agctgaagaa
     4081 tttgaaagtg aactgaaacg attactagaa aaagataaat tcattaattg ggagtgagtt
     4141 tcatgagaag aatagagaaa gaattcaaca agaagctagc cggttacgaa agagagttaa
     4201 aaaaattagg atgtctggat gatgaaacag gacttattcc cataagcaaa agacgatggc
     4261 atgtcatttg gtggcaacct gttacacttg caaaaacaat cgtgagatct tatcgattaa
     4321 cgctagataa tcaaaactta tgtattttag gagatgtaga aatcactatt tatcatgatg
     4381 gaacatatgg tatttctaaa gaagctgtcc caatttttat taatgactta ttgtctttaa
     4441 aaaagttaat tactattttc tatggaacac cttttaactt aaattttgaa aaaattcgct
     4501 gcgttaattt taacaggtat tgtgtctcta ttcctgaaat ttatgtcgag aaatttgaag
     4561 tattaataaa ctacttgata attttaagta gctgcttaca tgaagtaaaa aagcacgtag
     4621 aatatgatta acaagctttt cttctttcag aagaacacca atagattggt tgagttcgta
     4681 tacgctcacc taagagagga aaagaacgaa gtcaagcttc gttctttttt ctacgtcgga
     4741 gaaccgacgt agtgattcag acgcaaaaac catgcgtctg aatcaggccc gtttttagga
     4801 agggctagtg tgtttgattt atcagttctc acagccgtgt aatttctaat attccctaat
     4861 cgtatatcag tcactcggcg agtataacct aaaacataac ctgtcggagg tcaagggcaa
     4921 gtggcatcgg caagccgacc cttgtcctcc gacagaacat gttttctagt cggttatgcc
     4981 gaacgaccga tatacgaaaa gggaatctaa ccagaatttc ccctctgacc tttgaaaact
     5041 gaaaagcgaa atcaaacaaa caggccttcg tctaaaaacg agcctaatca aaccttaggg
     5101 gttgcacccc taacacccgg cgcctcactc tctcaaccga aaaggttggt tggaaaagcg
     5161 ttgacaaaaa catatttatt tattggttgt ttgaatataa aaaatgaaag aaggaaagca
     5221 aaatgctaac aacaaaagaa aaaaacagat ttaaaaaaat ggtagaagga aacaaaacgt
     5281 ttcattattc ttatgtcgat agacttagac aagatgtaag atattacgtt aatcaatgtg
     5341 aatcagctgt taaagctaga gaaagcatgg aaatcttaga atttatctac agtctatttt
     5401 ctgataaaga gttaccagca tggtacacaa aagccgacct tgaaaatgat aaaaaatcaa
     5461 ttgaaaaatt ggaacgctgg gcagcttagc tgttcagcaa caatcagaaa gggcgattag
     5521 catgaaattt atcaaagatg taacgacatt agaagaattg aaacgcgtat ataaaaagct
     5581 agctttaaag taccaccctg acatgggcgg tactgataaa gaaatggcgc aaataaataa
     5641 cgagtatgaa gcgttgttcg ataagcttaa aaatacacac aaaaataaag aaggtgagta
     5701 ctaccaaaaa gaaacgacag aaacaccgca agaatggcaa gaaatcatac acaagttgtt
     5761 aacgctaaaa atggaaaatg tttcaattga agtgataggc gcattcttat gggtatcagg
     5821 gaacacaaag ccttataaag aagagttaaa agctttaaat atgaaatgga gcaacaacaa
     5881 aaagtcctgg tatttagccc ctgatggata taaaaaacgt agtaagaaaa aatacgatat
     5941 gagcgatatt cgcaatatgt atggttcgca ggtagtgaaa gaagaacaag tcaaacaaaa
     6001 gacattaaca gcgtaaatag gaattgaatt acattaggaa aaaattaggt tctacgtcca
     6061 aagaggtttt acctctttgg acgaccaaat aaaaaagaaa gaaggaatca aaaatgatta
     6121 aaacaagtgc agtaggaaga ttaacaaaaa gttcagaact aagagtaacg tcaacaggga
     6181 aggccgttgc taattttgca ttagcttgca aaagagaaag acttgataaa gacgggaatg
     6241 cccaaacgac ttttattcaa tgtgtaatct gggggaaacc tgcagaggtg ttagcaaaat
     6301 atacgcaaaa aggttcatta attgcagtaa atggtgaatt acaatcacgt tcttatgatg
     6361 atcaacaagg acaaacgcat tatgttacag aattagtcgt agcaggcttt gaatttttag
     6421 aatcaaaaga aactgtacaa aatagagaac aaaaacaaca aatgccagaa atggcgaacg
     6481 ccgaaccgtt ttatggtatg gatgatatgg aaccgcctgc ttatcattaa aatttatctg
     6541 aaggagggag aaaatgaaaa agttaatcgg taaaaagtgg ctgctgctta cagcagtagc
     6601 cactttttta ttatcaggat gcgcaagtct tgaacaaaaa gcacaggata gtgtaaaaga
     6661 agttactgaa aatgttactc aaactatttc aaacgatcaa cgtataccag ctgattttgt
     6721 taggcacgtg gatggcgata ccacagtatt aaaaattgac ggaaaagaac aaaaagttcg
     6781 gtttttatta attgacacac ccgagactgt gaaaccgaaa acaaaagttc agccgttcgg
     6841 attggaagct agcaaacgta caaaagagct tttgtctaat gcttcagaaa ttacttttga
     6901 atatgatcaa ggcgataaaa cagatcgcta cggccgagcg ttgggttata tttttgtaga
     6961 tggaacatta ctacaaaaaa cacttgtgag tgaaggttta gcacgtgtgg cctatataaa
     7021 agaacctaac actaaatatt tgtcagaact agagcaagcc caagaacagg ctaaaaatga
     7081 gtcactcgga atctggagca taccaggtta tgtgacacaa cgggggttta gtaaataaat
     7141 aagaaatcgc tatgaattat tgaaagtttt tggcgagaaa ttataacaca ttatgttgtt
     7201 gttaacaaca taatgtgtta taattatata cataaaggag gggtaaaggt ggctactaca
     7261 aaaaagaaac ctatacatgt aaatgttgat gaaaatttaa aagaagaagc agaacaatta
     7321 tttgatgatt tagggttaaa tatgacaagt gcaattacga ttttcttaaa acagtctatt
     7381 aatgagcaag caattccttt tatgattaat aagggaaaca aagagactct acaagcatta
     7441 aaagacatta aagaaggaaa tgttcatggt ggattttctt ccgtggagga tttaatggag
     7501 gatttaaatg cttgaaatat tttacacgaa ccaattcaaa aaagacttta agaaagcaaa
     7561 gaaacaagga aagaatttag agaaattaaa agaagtatta gtacttttac aagaacagca
     7621 aacattgccg ccaaagtata aagaccatgc actaacagga aattatatag gtacacgaga
     7681 gtgccatatt gaaccagatt ggttactgat ttataaaatt gacggtgata aattgatcct
     7741 aactttagcc cgaataggct cacatagtga attatttcga tgaatcggaa agatattaca
     7801 aatcaataaa aaacactact aaacgaccag acgaatagta gtgttttttg attatgcgat
     7861 tgtactaagc aattttaaca caataaaaaa taatcatgca agactagggc gcaacaatct
     7921 attatagacg tgcgcctttt ttagttcggg aaaagggaat gaatccgttg ttccgcggcg
     7981 gtgcggtagc ccttgctcca aaaccaacgg attaactgct gcactcaaca cgagtcaatt
     8041 aaacgttctc tacccattcg ttccgattgg ggaacgtttc cttttcctac tcgttcctcg
     8101 cttaacggaa aaggccattg acacgagttg ttccttgtgg gatcacaagg aagcgttccg
     8161 cagtgattaa catttctagt aggtatttca tacctactag aaaacaaagt caagggcgtt
     8221 ccggtcaaaa atcccttttc tgatgtcaga acagtgtcac gcgacctttt tcgttttcag
     8281 gccgttcatt ttgcagtgtg taacgcgctg ctcactgttt gctacagtac cacagccagt
     8341 cgaaaaaaca agggtatata aacgagcaag ctcgcccttg ttttttcttt atgtctatgg
     8401 tccttccacg gcacagcgag cgacacgaac acactggcga aaatgaacaa cctaaacaaa
     8461 aacgaaaaag ggggtagact acaccgtttg acgaaaagga atttttgacc gtccagcttc
     8521 acttcctcaa acgcctaaca aaaatggttg ttcaaaaccc aaaaacctga aaggaggtgg
     8581 ttaaaatgaa tgagctacaa ttaaaacttg acctagaaaa ggcacaactg gaataccaaa
     8641 aattaagcca agcaatcaat gaaaatgata ctgtcacgct gttattaaat tatggttgtt
     8701 taaaaaatgc caatgaccgt ttaaaccaac tatcattttt gttgaaccac attgaatgga
     8761 aggacgtgta agaatggctg aaaaaatact gtgtgacaat tgttgaaacc ttagaggtag
     8821 ctgtatatgt ttatgcagaa agcgaagaac aagcacatga attagtttct aacatgtatt
     8881 acgaccaaga aataatctta acagaagaga atcacgaaac aacagaattt atcatttaaa
     8941 acaacgaaag aggtgaaaaa gatattttag aggtattgaa ttatatccac gaaaaaagca
     9001 caaaaaaaga aaccttttgc cactaacaaa agatttctaa agaattggta gttatacctg
     9061 aataaatttg taattcaagt ataacgtatc tcatgacctt tgacaagtga atttccccca
     9121 acctcttatt cataagaggt tggactaaca aaagaaacga gggaacgtta tggattttca
     9181 aaatatacaa actgtaaaag aattaacaca cgcactcaaa caatacaatc aaaatctagc
     9241 aaccactatt tttgacgaca cacaaggcta tataagtgta gcattcaaaa aggacaagat
     9301 agaaaagaac ggaaacgact ttcaattttt atgtttctat cctaattatg aagtagccct
     9361 ttatccagtc gtgacattac tttcttatct tgaaaagcta ccagaacaag caaccgttgc
     9421 gattaaagac cctgacggta actactcgtc cctttatcta accattgatg aaacgataga
     9481 taaagggacg aaatacaagt ggctagtaat tagcgacaga ctttcttatg aacgctttta
     9541 cttaactagt caattgacta tacaagagca agcactggaa aagatgttaa aagaactaga
     9601 aaccgaacct aacaatagac tagaagccat tcacgactgg ctatgccaac aaaccgataa
     9661 agaccttttg gttggtattt taaaggaaaa tagaacattg aaaggggcgg aagcttactg
     9721 cactcaaaag gcctatgaaa tacgtaatgg caacggggca atggctagtc atattactgt
     9781 ttttaactgg gttagagaat attatacgct tgacgaactg ccaacggata agccaaaaat
     9841 acaagcgaca ataaaaagcg attatatcaa tcaaaaaata cagcgaccaa caccagtaga
     9901 aaccaagaat aataacgaaa tagatttgtt tgagggggaa gatttttaat gaatactact
     9961 gactatgtag agaataaatt acaaccgcca agagcgttct ttgaatggtg ctactcttct
    10021 tttcggacat atgtatggaa aaataaaaat gaaaccattg tttcttcaac aagaaaacac
    10081 gattggataa ttgagaaaaa attgagaaag aactctcgat tgacctttta tgattcaagc
    10141 agttgttttc agattatact aagtacaagt aagcgaattg aagtacagac ctacaaagtt
    10201 atttcagagt atgaaaatgg agtgcaatgt tttcgtgaac agctagaatg tattgaaata
    10261 ttttcaaata atcaacatat caagattgga aagatatgtt tacctgtata ttatggttac
    10321 aatatgggca tagccttata tcctaatgaa tggaaaaagc gtttagaacg tgtgtcagag
    10381 ttgaaatatc taaatttaga gcgattaaac gttgataatt tagctaccac gtacaaatat
    10441 agaaccttga tagaatttgc tcagaaaata aatgctcata aattagcata tgatgttatg
    10501 agtggtgcag tagacatgcg aatacttaca aagaactgtt taaggaagta caaaacattt
    10561 ttaaagaata cagacaatag tctaaaagag atacagctaa aacaaacatt tgaaagctta
    10621 aacataccaa tggtaaaagg gattgaaaaa tacgtattaa agagcgacat atccgatttt
    10681 ccaaatgaga ttggcgctgt taagtttcag aattggttag taaagcaagg aaagtcattt
    10741 aagtattatc aagactacct caatatgtta acgctcctaa aaatagaaat aaacaaacgt
    10801 aatcagttac cgccagattt agaagtcgca cacgattgtg cagtagatag aattaatcag
    10861 ttgaactatg aaaaaaggga taaagaaata aatgaacgtt tgaaacaact aaggaagtat
    10921 gaacgagata ttgatgggta cacatttgtg ttacctaaac gagcaaacga cataaaaaaa
    10981 gaaggaaaag cactaaacca ttgcgtagca agctatataa gcagacacgc aaagggagaa
    11041 acaacgatta tcttcgtaag agagaagaag aaccctcaaa aatcttattt cacattagag
    11101 tacaactaca atagggttgt tcagttacaa ggaaagaaga atcgacaaaa ggtaccagat
    11161 gaattgaaac aagcggtaga caaatgggtc aaggtcatta aagattaaac gttaggaatg
    11221 ctaagaaaag accgttctta gcattcctaa ccgaaatcta ggacaaaatc cattgttccg
    11281 cggcggtgca gtcgcccttg ctccaaaacc aatggatttt ctgctgcact caacacgagt
    11341 caattaaacg ttctattccc attcgttccg actggtgaac gtttctcttt tcctactcgt
    11401 tcctcgctta acggaaaagg gcattgacac gagttgttcc ttgtgagacc acaaggaagc
    11461 gttccgcaga tttaagtttt ttctgaagca gtttcactgc tacagaaaaa aggcgttggt
    11521 tctatggcat ttttctgtca ctcggcccct tcttgcggtt atcatttctt cagtgagccg
    11581 gccttgtgta cttgcgaatc atacaacagc cagtcgaaaa cacaagggta aaatgcacga
    11641 gcaagctcgc ccttgtgttt tctttgtgtc tgatgtctgt tctctgcgca cccaaaacct
    11701 caaactcact gaagaaatgg atcataacag caaacaaaga aaggagagga ccacagaaaa
    11761 ataccgaacc aacgaagttc aaaagaagca ccccttccta aatcttcaaa aaaaatgagt
    11821 tttgaaaaaa ataaaaaaca gaaagaagcg aataaaaatg actagacttg aacaagcaca
    11881 aggaaaattg caacgactaa aaagagaaag cgaagaaatg caccacttaa tcagggcgga
    11941 acatgaccgt atcccttttg gacagcctaa cattattggc cgtggggaca tttataagaa
    12001 agtaaatggc tatcatgatc gtgcaattaa attactgaag gaacaagaaa aacaagaaaa
    12061 aagagtggaa atgctggaaa aggtagaaga ttttaaggaa aaaaacgagc taatcaaaga
    12121 cgttcatgtg gtcggtaaat caagttatgc gacggttggc gcaaaaacaa gtgtcaataa
    12181 cattgattac tttaaaaatg aactaaaaga gcttgaaaag gctaacgaaa aagcaaaagc
    12241 ctataacaag acaaaaccag caatcaaagc gagaacgttc ggcgcagtaa tcacgaaact
    12301 aaaaaataaa attgcgaccc tggaacaaat gaaggaagct gatgaaaaca aggtggtgag
    12361 tgagagaaca aaagagctga tagaaagcgg tgcagtgacg cagtggaaga aaaaaccaat
    12421 attctatttc gttaaagggt tgcgaaaagt agcactagaa attgacgaaa atggagaatt
    12481 cttcatttct aactattatc cggcttgtac agatgcagat aaggaattta tcagtaagtt
    12541 attagatcct gcagctgaaa gcagaaaaaa agaaaccttt tgctgaagac aaaagatttc
    12601 taggacgtta actatttata gtcatgaata agtatagcgt accttagaaa aatattcaat
    12661 gggttcctgc cataacaatg gagttatggc aaggattcat tgccactcat agaatagaac
    12721 aattatctaa cagtaacgaa ttaaaaaaaa tattgctttt ggaggaatac aaatgagaag
    12781 attaagtaat acgacaatgg ccaacgaaac tctaaaaaaa gaagcatggt atagaatgat
    12841 gcttaccgac ctatcaagtt ctgtcattga tgagttcatg gaaacgggga aatgccatta
    12901 caccagcaat tattttcaag gtgaaaatat tttagtcact gaagaaatag aggcaatcat
    12961 aaagacggtc gaaaagaagt atggcttcct ggtgtactat gtaactgaaa ataaaacagc
    13021 ggatggccag agatttttat cattattcta cgttggacgc gatactagtg attggcttta
    13081 ctgtcacagg gatttagaat cttataggca atatgtgtat gtagttaata cgacgaaccc
    13141 tgcattttca gaatttggaa tgattcaatt tgatccaata ctaggcagtc ttttacgaac
    13201 tgcatagata aaaaaatata cattttatgg tatcagttgg gctttttggc ccagctgaag
    13261 cctaacgtaa ggaattataa gaaaggtggg aaatgagtat ggaaatacgg gaattaatta
    13321 taatagaaga tccttgcttt tatcaatata cgacgataaa agaacttgaa tcgtttaata
    13381 cgtctgaatt aacgattgct cttagtgtgc ttagaaaaga gcagcgtcgc ttgaaagaaa
    13441 aagtgtatca ttctggatct ggcgatcaag tggataaaaa taacgttaaa ttaatcaaca
    13501 agtacagagc caatcttgaa aaactgctgt ttattaagca agggtatatt ccggatgcaa
    13561 tcacgcaaaa catgattgat acattgatca aaaacaataa aaaatccata cagaagattc
    13621 aaaaaggttg agtttagcaa ggcaacaaaa aacatttaat aaaagagagg atgtaaaaca
    13681 tggccgaaaa aattgatttg aataagttat atcatgaaag taaaaaggaa agaaccgaaa
    13741 aacaaacaaa aatggaacga gcgttagaag gagttgtgtg tgggtttcca agaaacacag
    13801 atgatgatcc aattttaaag aatagtaaga cccaggagaa taccaatgga tgaaaaaagg
    13861 aaagataaaa ttcaacggtt gttagcacta gcgaacgatg cgaacgatga agaatcaatg
    13921 acagctttag caaaggccca ggaattgatg ctagcctatg gaattgatga agaagaagtg
    13981 tttcattatc agcaacacca aaggcaagaa aaggttatag ataaagtgat ttacaaagga
    14041 agaccgcata aatgggtcta cagactagct gggatcattg caaagaattt tcgtgtgaag
    14101 aattactatc aaagttcttc agatatagaa ttacactacg taggtttaac aacagatgta
    14161 gagattgcag aaattacatt tttatatgca aaaggtagtg ttgccttttg ctcaaaagag
    14221 tttatgaaac aaccggaaat caagcgaaaa tataaacgaa aatggcaatt aaaacaagac
    14281 tatatcgaag ggtatttgac ggctcttagt tcagtatttg aagcccaggt acttacgaat
    14341 ggttacgaac ttgcattgca gttgcctgtc gcagtccatg aagaagtctc aaagctaggc
    14401 ttggttaaag gaaaagacac ctcacaccat gttgaaaact tcgatgcttt tcattctggt
    14461 taccaggaag gcttacagtt taagagtaaa gagttaatca actaataaaa ttattaatgg
    14521 aggaaagagt atgcctaatt tggattccta cttagaaaga tttgaaaact atcagaagga
    14581 acaagaagaa ttaaatgaaa tttttgatcc ggacgatagg aggtgcagag tttgtggatg
    14641 tactcaattt aatgcatgcc cagggggttg ttattggatt gaagaagact tatgcagtaa
    14701 atgtgtatag aaagagaaca aaaaaagcag ataaaaaatt atttgctttt tttacttata
    14761 tattttatac gataaaaaaa cgtggttttt agtttgaaaa ccctgtaaga ttaatatatg
    14821 gacccattta atgtaaacat aaaaggtgtt cgttttctta ggggggtatt ttaataaaaa
    14881 ttgaggagtg aaaaaatgaa taagtttcaa agttggctta tggccaataa aaatctgtta
    14941 attacaagtg tagtaatgtg tcaagtgtta gtcttaagca tagggataat caactttcct
    15001 tatattgatg acaccggcag gcaaattgat ggcaatacgg actttgctag aagttattct
    15061 cgctggggta gcgaaattgg ttcttggtta gttcaaggga gtagacacct tacggatatg
    15121 gggttgtgga cgcatattct ttctggattc attgtatcag taacagggat tatcattgtt
    15181 tactcttttc ataagaagct atctctttta gctttaacag cagccatgtt ggttggcttt
    15241 aatccttggt ttttacaaaa tttaagtttc aggttcgata gtccttacat gtctttgagc
    15301 cttttatttt cagttcttcc atttctattt tggaataaaa ataaagtctt atttttagtt
    15361 gtttctattt taagcatttt tttgatgtgt aatacttatc aggcgtcttc ggggatctac
    15421 gtcgtgatgg tgttagcatt atcactgaag cagctgttag acaatcaatc gtttattgct
    15481 gtgcttaaaa aagcgattgt agctatgatt agctatataa gcgccatgtt tctttatctt
    15541 attgaaacaa aatttaatcc tgaaatagca acgcgaggcg gtatgacaac aatcgcgagc
    15601 gttaaggata tacccaaaac aatcctggta aatagtcaaa tgtatctgtc aaaaataacc
    15661 gagcagagta caaagctatg gatacttttg ttttttattc tagtaatttt ctttgtttta
    15721 agtacggttc ttaatgcgaa agtatcgcct gttaaaagct ttttatacgc agtattattt
    15781 ctatttttag gctctatttt gagttacggc gtctttctaa tctttccgga aaagcttttg
    15841 ttaatttcgc ctagatacgg ctatggattt agtttttttg tagtttcaat tatcatgctt
    15901 actttgtcaa atacttctaa aaacgtgcta ggatatgtgt caaaaacagc ggtttgtctt
    15961 ttctgtgtct accttttatc ttttacattt gtttatgcta gtgcgttaag ccaccaaaag
    16021 gaatcttttg aacgacaaag tatgatatta gccgatgatt taaaagacct agtgaaccgt
    16081 gacacggtga ccgtgcatag tacgtcttta tttaaagatt cgccagtatt tgctaatagt
    16141 tcgaagaatt atcctatatt aaaagaactg gttccaccaa atgaagccct ttattggcca
    16201 aatcagtttt tatttagaac gtacactggt ttaaatgtga acatggagat ctttgatatt
    16261 aacgcgttga ataaagaaga aagtgagtta atgaaatcta attattacca tgacatttat
    16321 gtaaaagatt cagaagtatt tgttcatgtt aaataatcct gaaataaaag taatatcaag
    16381 gcaattgaaa ggccggggta gatactgtaa aataaaaata gctatttcag atttaactat
    16441 ttacttacag actgaataaa ttgagcgttc agttctatga gtacttgtta gaaacgaaag
    16501 gctaaaataa gttataacct tagagactgt ctccccccaa gagacagtct tatatattgt
    16561 ttaatagata attactttac taaaatagga taaattcaat cagaaatgta tactttgtaa
    16621 ggaaaaaatc atagctgttt taggatatta tttgctattt tatgtatttg ccaattacaa
    16681 tggtattgta aataaataca taaagtagca ttagtaggtt ttgggggata tagatttgga
    16741 aaatattttt gatttaaaca agggattatc aagaagaaaa tattggtatt atcagtgtat
    16801 actgtttgtg tttggattag ggatagaaac tttttcaatg tttgtataca aaaagtctct
    16861 gattgaaaac aattatttaa aatatttcgt gataaatggt ccatttgatt tttataattt
    16921 tcttaggatc gtgttcttta taatgattat tataggtttt ctgttccaag caagaaggtt
    16981 tgttgatatt gggtggtcga aatttgtagc tttactaagt ctgttaccgt tagttggtat
    17041 tatcacaaca gggatttgtt tatttaaaaa gggaaaagac aataaaacag atagcagtca
    17101 agtttcttga ttgctatctg ttttatttat taagagtatt gtctattaaa tgaatgacaa
    17161 gaacttgcat tgtaagcagc ttgaatcgca gctgaccatg agagtttatg gaatgctact
    17221 gcagaccaaa ttcctttcat aatacaagca ccttgttttg ctttagcttg ttgatattta
    17281 ttccataaat gtttagggac tttacttttt gcaaagttgt cctaataacc atcgtcactt
    17341 tgggcatatg cagtagtaaa gtttactaac ggataataca tgataaattt aaaaggtagg
    17401 gtactttttc gtacaagaat gattttgtaa gtatgaaaca agctagttat atttcactag
    17461 ctattcaatg aaataagttt ttaaagtaga ttgttgatag atatattaaa attttttttc
    17521 atgtaagagt atgctaaaat tgatgttaag aatggttttc cctagtaata ggtaacatca
    17581 aaataaagga aggtgtaatt ctatacctat ggaagaaaat aatatagaaa gcaaagacaa
    17641 tctgtttaaa gagattgggg aaaaaatgta tccgcttttt tttaatcgta attttggatt
    17701 agcaacaaaa agtgaaattg aaagcatgct cttttactcg tatctaaaac ataagataaa
    17761 tagtaatagt gattactcag atagaacctt gtctagagag ctaggaatat cggaagttaa
    17821 agtaaggaat cttaagagaa catgttatgc aagatatgaa gatgcaattg attttcctaa
    17881 attactaacc tcgctgtcca aagaaggctc aaatctaatg ttttttaaaa tctatactga
    17941 caataatgat gtattatgta ggatatctat ttccgaggta gtttattatg atgaacttcg
    18001 aacacaatta aacaatagtg gtattctttt taaaaaaaat ccaggatcaa attatgtaga
    18061 gttatcaatg gcagatgcaa taattttctt tgatcaggat tctatcagca aagaaagctt
    18121 agatttgaaa aatttaatag ataagatgaa acagttgaat tttaaaacta atactaaaat
    18181 ggatattgat aaaatattag aaggaactaa agctggtaga atgaaaaatg tcgttgaggc
    18241 tatgatgaag ctttccaact caatataaac aaagaggtca gaaacttcta agttttttga
    18301 cctattgctt tttcgtgtgt aactttttta tttaaatggc ataagttttt atcgatgaat
    18361 ggcaaaagct accaaaacaa atggaaaaaa ccagttgtat attacaactg gtttttttga
    18421 acgaaatctc gttcaatgga tagcttaact ataatataag tatacaaaat atgcaagcgt
    18481 ttacattaaa taaaaaattg aatttggtag tagggtacat atgattctat ttaatagcta
    18541 acaaaatttt tttctctatt tcacgtgtat tattagaaat catatcttta aacgggataa
    18601 ctataccatt attttttaag tcatcactta gatatctgga gtctatgtca ttatatacaa
    18661 tcaagtctgc cttgggtatt tcagtacttg aagtatcata gcttataaaa tcaacagtaa
    18721 catcagacgg caaattcgtt ttaattgttt cttgaaattt ttgtgcttgt ctcaaaggca
    18781 tagttgtact atttccataa agacccatac cctttaaatt tacagaatat atccatacta
    18841 tctttttcat ttgtattccc ccttctaaca tattatttac tttaaatata tcactttgta
    18901 aaattaatac aagaaatatc tacaacgatt ttaagcaatg tgatataatg atcaatgaaa
    18961 gcgcttactt gactttgttc cgtatactta actatacata ttttttaata cctacttaat
    19021 atgtaacaat tactctgtac ataaggtagt taaacaacta gaaaattatt ttggaggaga
    19081 tatttatgag taaattatta cgtgaattaa cagacaagga aatacaatct tcgttatcta
    19141 atggtggatc tacatgcagt atgtataaag cgctattacc tggacatatt gctgcatgtg
    19201 cagctagtgc atatttttat ggttcaagtg gctgtaccgc aatgagatat gtttcagaat
    19261 atataagaaa gaattgttaa ccattcaatt tttttaatgt agtttatata gcatgttaat
    19321 ttgttgttta actatcttat tcttaggagg atatatctat gcttagaaat ttttcagtaa
    19381 taattatatg taaaaatgaa gaaagagtaa tcaagcgatg catagaatct atacaaaaaa
    19441 atttgaaaaa gaatgatgaa atcatagtag tggataccgg gtctacagat aatactataa
    19501 aaattatatc tagtatagaa aatgtaaaac tattcaatta tgaatggaaa gatgactttg
    19561 cagatgcaag aaactttggt atttcaaagg caaaaaaaga ttggattttt tttattgatt
    19621 ccgatgagat aatgtcaaaa ggatgtttag agcaattaaa tgctagtata aatgaagcaa
    19681 tggcctactc tgaaaataat gaaaagcttg ttttttcgcc taaagcagtt aatacggatg
    19741 attctgtatt ctacaatgca ggaagaatat ttgctaatga tggaaacatt aaatacgaag
    19801 ggtgtgtaca cgaatatcct gtcgtgactg ataaaagttt aacgcttgtt tctttaaaac
    19861 ttcctagagt tatagtaaga catgatggtt atgaaaaaga ggtacgagca gacaaaaata
    19921 aaacagtaag aaatataact ttaattaaaa aaatccttca ggaagaacct aacaactctc
    19981 agtattatta ttattactac agagattcaa aacccttgat tacgattgaa gaatacgaac
    20041 aagggatgct aaattttttt gaaaaatttc ccgatagtcc ttacattgat caagtagtaa
    20101 gagatctagc ttatcattat atacaagttg gtaaaaatga attagcagag gaatatatta
    20161 atatgttatt tgagagcgca gaaaatggag cgaaagaaaa ttatcattta gcaatattat
    20221 acacagggat gaatgaaatg ggtaagataa aagcccaaca aaaagacata ttaaagttac
    20281 ttgtatatgc acatgataat gttttacagc aagaggaaga gttgtttgat aagggatata
    20341 tattcgatga tttaataagt atactgtttt ttcagattga ggaatacgaa accgcatata
    20401 aaatatgtga aaaacttgat tcttgtggct atgcttctaa catttcaaaa atgattagta
    20461 aacttaaagt ggttctaagt cctaattcta aaaattttag gtaaaaggag tcaattctta
    20521 gataccataa cttaatttta ttataagtaa atatcaactt aaattaataa tgctttttag
    20581 ataaggaaga taaaaatgaa atataaagtt ataccacaaa taggtgaaga agattgcggt
    20641 gctgcatgtg tagcaatgat agtagaaaac tatttcaaaa aaaaaatatc aatttgtgaa
    20701 ataagaccgt taattaagaa tacaaaaatg gggacttctt tctttgactt aaaaagagga
    20761 ctaaagcgtt tgggagttga ttgcaaaatt tcgcaggcag taaagcagaa agacgttttt
    20821 gaagaaatta catacccatt tattacacaa atttacaatg agacaggtat tcattacatt
    20881 acagtattcg aaaaaaaaag aaataaatta attgttggag actctagtaa aaatagacag
    20941 ctttcaattt caattagtaa gtttctaaaa aaatggattc catatgtttt agaaatggat
    21001 tttaaaagat caaatatagt ctatcaaatt gaacaaaaag ctaaggaaat aagtatttgg
    21061 aaaatcatga atagtctaaa actccaactt tttctatcat tatgtctatc tattatagtg
    21121 tatgtagcta gtgtaataat agcaaatata tatactttgt attttaacat tttgatacca
    21181 caaaaccttg gagagttagc tttacagtta atgtttatat atatattaat aaactttttt
    21241 agcttttctt taacatttat taataatttt atttataact caatgagtaa gaaaattgat
    21301 gaaaaaatta taggaaaata ttttaacggt ttactaaaca aacctaacat ggctatagaa
    21361 tcttatgaaa taggagaatt gcttactaat ctttctaacg ttattttgat caggcaaaga
    21421 tttctgacat atctgcaatt acttccaatc agtgttttga ctattactgg tagcttttat
    21481 ttattatacc tatctgatga aaaattatct ttttttgtta tattgctagt aattgttctt
    21541 agtctaatta tatatttatc tgatagtcac tatgaaaagc taagcaagga attaatagaa
    21601 acaggtcaag attttaacga ttctgtaatt gatacattta ctaatgtaag tattataaaa
    21661 caactgggac ttgaaaaaga atttggtaat agaggtaaaa aaaagcttac caattatatt
    21721 atttctcgaa caaaaatgtt gaattttgac tcgattctaa atcaaataaa gtcttttgta
    21781 ctatcgtcat tcaatattat tttattttca actggtgtat atttgattac tttaaatcag
    21841 ttatcttcag gtgtcctgct tacattcaat gctctactag catacgtaac taatcctatt
    21901 ttgaatcttg ctaacttaca gtcggtgtta gtacaaggaa aagtagcaca agagaagctt
    21961 tataatattc tagaatcaca aatagtttta tatggaaata agtcattggt aatagaaaat
    22021 gacgatattt ttatcaactt tgatttagta tcatatgatt atgattccaa ctctaaaatt
    22081 ttaaaagata tctcaattaa tatatgcgga aacaacatcg cattaagcgg aactaatgga
    22141 gttggtaagt ctacaatagg aaagcttatt gctcgtctct atattccaga tagtggaagt
    22201 atagaaatca atgggcaaaa cttgattgac atatcagaag agtctgtttc taaaaatatt
    22261 atttatgtag atggtagaga aagtctcttt tcttcttctg tatcagataa tattaagctt
    22321 gggcgaaata ttaaggataa agatatatac gacacgttaa gtaaactaaa agctaattca
    22381 atttttcaaa atcttgattt tgaaaatatt agtgataagc aattaagttt agggcaaatg
    22441 caaataataa aggttttaag agcaactctt tctgcaaaaa atatatatat ttttgatgaa
    22501 atcactaatg ggcttgatga atgtgtaaag caaaatgttg tagattattt atttgggtta
    22561 aaagggatga agatttttat aacacatgat aaagaggtta tttcaaagtg cgagaaagaa
    22621 tataccgtaa aagacatgaa aattgtgagg aggcgataac atgaaaaaaa tttttattag
    22681 tatctctata cttgtaataa caggaattct aggaatgctt atgtataaca acattactag
    22741 tgaaaaaaca tattatacag aaactaagtt tggcaagatt tctgaattag ttaaaaaaaa
    22801 tgatagaata atactatttg tcgaacaaga tggatgttca tcgtgtaaac ttgtgaagcc
    22861 aattataaat aactatgcaa aagaaaataa aggaatagtt tattccgtta cagctaataa
    22921 agaaaaagac tacaacgtgc aatcagaaaa atatcatata gaaggtactc caacattaat
    22981 atacttcaaa gaaggcaaag aagtttatcg tacaaacacc ggttttaccg aggatgagtt
    23041 tcagaatact ataaagaaag tggaatttta atatgaataa gaaggaatat ctaggtatca
    23101 tactaattgc cttagcaagt ataagtatta ttctttttct atacagtaat aacagagaac
    23161 ttaaaaaagc taatacaatg ctacagctcg aaaaacaagt cttaatagac aacacagatt
    23221 cttttgaata tctaaattcg attactgtag atacttttga aaatagaatt aaaaataagg
    23281 aagatatttt tgtctatata ggaaatagtg agtgtgcgga ctgctcaact ttttcaaaaa
    23341 acctaaagaa tgaagtgaat aatttccctc ttaagaattc attgtattta gtagagataa
    23401 gtagacttca tagaaataag aagaaatgga cagaatttaa gttaaaatat ggatttgatc
    23461 agactcctgc atttatattg tttaaaaatg gaaaagtgaa tagtatgatt cagtgggatg
    23521 aaaaaaaggg tttaagttct gattcattcc ataggtggtt agaagaaaat agaaatgata
    23581 tagaaaatct tagttaataa ttcagaagag ttagatcata tgtattgaat aaattcagaa
    23641 acaatttagt gcatagttaa aaagcatgta agctattcct tggaaggtca gaaacttaaa
    23701 agtttttgac ccttttttta ttcctcattt cacaactctt atagtggctc agttaattag
    23761 gtcagaaagt tactttctct tgaggacaac agagtatact tatttgtcct aatatgattt
    23821 tagcagtata attgacttgg tgaataggtc atttaagttg agcataatag gaggagtaaa
    23881 atgaaaaaat ttatttatcg agttttagaa aatgacgaag tggtggctat ttttaatgaa
    23941 caacaatatg cgcaagattt tatcgcttac gaaaagacaa tttctgataa gcaatttgaa
    24001 attgaaaaag tagatatagc tgattggtta ttgcaaccga gagaatttta gaggttggtt
    24061 gaaaatggct aaaattggtt atgcacgtgt cagtagcaaa gaacagaact tagatcggca
    24121 attacaagcg ttacagggcg tttctaaggt cttttcagac aaattaagcg gtcaatcggt
    24181 cgaacgtcca caattacaag ctatgcttaa ctatattcgt gaaggggata ttgttgtagt
    24241 tactgaatta gatcgattag gacggaataa taaagaatta acagaattga tgaatgccat
    24301 tcaacaaaaa ggagcaacgc ttgaagtttt gaatttgccc tctatgaatg ggattgaaga
    24361 tgaaaattta aggcgtttga ttaataattt agtgattgaa ttatataagt atcaagcgga
    24421 atcagaacgc aaacgtatta aagaacgaca agcccaaggg attgaaatag ctaagtcaaa
    24481 aggaaaattc aaaggtcgtc agcataaatt taaagaaaat gatccacgtt taaaacatgc
    24541 ttttgattta tttttgaacg gttgttccga taaagaagtt gaggaacaaa ctggaattaa
    24601 tcgcagaacg tttagaaggt atcgtaaaag atataacgtg acagtcgatc aaagaaaaaa
    24661 caagggaaag agggatagtt aatgagtaca gttattttag ctgaaaaacc aagccaggcg
    24721 ttagcctatg cccaggcatt taatca
//
LOCUS       DACERL010000042        24337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.043, whole genome shotgun sequence.
ACCESSION   DACERL010000042 DACERL010000000
VERSION     DACERL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.043"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(132..2477)
                     /locus_tag="IXF54_001693"
     CDS             complement(132..2477)
                     /locus_tag="IXF54_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1404953.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="IXF54_001694"
     CDS             2756..3658
                     /locus_tag="IXF54_001694"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1404954.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="IXF54_001695"
     CDS             3869..5644
                     /locus_tag="IXF54_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1404955.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="IXF54_001696"
     CDS             complement(5710..7296)
                     /locus_tag="IXF54_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1404956.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="IXF54_001697"
     CDS             7547..7771
                     /locus_tag="IXF54_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1404957.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="IXF54_001698"
     CDS             7913..9664
                     /locus_tag="IXF54_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1404958.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="IXF54_001699"
     CDS             9664..11448
                     /locus_tag="IXF54_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404959.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="IXF54_001700"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="IXF54_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="IXF54_001701"
     CDS             12732..14618
                     /locus_tag="IXF54_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1404960.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="IXF54_001702"
     CDS             complement(14659..15561)
                     /locus_tag="IXF54_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1404961.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="IXF54_001703"
     CDS             15791..17194
                     /locus_tag="IXF54_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1404962.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /gene="ascB"
                     /locus_tag="IXF54_001704"
     CDS             17194..18627
                     /gene="ascB"
                     /locus_tag="IXF54_001704"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1404963.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="IXF54_001705"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="IXF54_001705"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1404964.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="IXF54_001706"
     CDS             19911..21638
                     /locus_tag="IXF54_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1404965.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21711..22004
                     /locus_tag="IXF54_001707"
     CDS             21711..22004
                     /locus_tag="IXF54_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1404966.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="IXF54_001708"
     CDS             complement(22098..22958)
                     /locus_tag="IXF54_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1404967.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="IXF54_001709"
     CDS             23050..23478
                     /locus_tag="IXF54_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1404968.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="IXF54_001710"
     CDS             23640..23816
                     /locus_tag="IXF54_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1404969.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="IXF54_001711"
     CDS             23843..24289
                     /locus_tag="IXF54_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1404970.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DACERL010000043        24033 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.044, whole genome shotgun sequence.
ACCESSION   DACERL010000043 DACERL010000000
VERSION     DACERL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24033
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.044"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(24..722)
                     /locus_tag="IXF54_001712"
     CDS             complement(24..722)
                     /locus_tag="IXF54_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1404971.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IXF54_001713"
     CDS             complement(715..2067)
                     /locus_tag="IXF54_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1404972.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IXF54_001714"
     CDS             complement(2290..3690)
                     /locus_tag="IXF54_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1404973.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IXF54_001715"
     CDS             3851..4588
                     /locus_tag="IXF54_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1404974.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IXF54_001716"
     CDS             4713..5192
                     /locus_tag="IXF54_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1404975.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IXF54_001717"
     CDS             5189..5956
                     /locus_tag="IXF54_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404976.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IXF54_001718"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IXF54_001718"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1404977.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IXF54_001719"
     CDS             complement(6859..7461)
                     /locus_tag="IXF54_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1404978.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IXF54_001720"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IXF54_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IXF54_001721"
     CDS             8142..8726
                     /locus_tag="IXF54_001721"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1404979.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IXF54_001722"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IXF54_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1404980.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IXF54_001723"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IXF54_001723"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1404981.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IXF54_001724"
     CDS             10627..10806
                     /locus_tag="IXF54_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404982.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IXF54_001725"
     CDS             10828..11853
                     /locus_tag="IXF54_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1404983.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IXF54_001726"
     CDS             12028..13272
                     /locus_tag="IXF54_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1404984.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IXF54_001727"
     CDS             13425..14372
                     /locus_tag="IXF54_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1404985.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IXF54_001728"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IXF54_001728"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1404986.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IXF54_001729"
     CDS             complement(15274..15516)
                     /locus_tag="IXF54_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404987.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IXF54_001730"
     tRNA            15637..15709
                     /locus_tag="IXF54_001730"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IXF54_001731"
     CDS             complement(15828..16466)
                     /locus_tag="IXF54_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1404988.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IXF54_001732"
     CDS             complement(16589..17221)
                     /locus_tag="IXF54_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1404989.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IXF54_001733"
     CDS             17572..17871
                     /locus_tag="IXF54_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1404990.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="IXF54_001734"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="IXF54_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1404991.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20803..21288)
                     /locus_tag="IXF54_001735"
     CDS             complement(20803..21288)
                     /locus_tag="IXF54_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1404992.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21768..22409
                     /locus_tag="IXF54_001736"
     CDS             21768..22409
                     /locus_tag="IXF54_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1404993.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22428..23063
                     /locus_tag="IXF54_001737"
     CDS             22428..23063
                     /locus_tag="IXF54_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1404994.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23081..23896
                     /locus_tag="IXF54_001738"
     CDS             23081..23896
                     /locus_tag="IXF54_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1404995.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8319 a         4090 c         4777 g         6847 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
    20581 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
    20641 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
    20701 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
    20761 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
    20821 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
    20881 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
    20941 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
    21001 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
    21061 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
    21121 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
    21181 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
    21241 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
    21301 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
    21361 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
    21421 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
    21481 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
    21541 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
    21601 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
    21661 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
    21721 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
    21781 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
    21841 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
    21901 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
    21961 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
    22021 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
    22081 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
    22141 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
    22201 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
    22261 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
    22321 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
    22381 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
    22441 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
    22501 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
    22561 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
    22621 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
    22681 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
    22741 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
    22801 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
    22861 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
    22921 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
    22981 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
    23041 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
    23101 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
    23161 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
    23221 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
    23281 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
    23341 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
    23401 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
    23461 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
    23521 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
    23581 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
    23641 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
    23701 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
    23761 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
    23821 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
    23881 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
    23941 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
    24001 acctcttggt aaacggtgtt caaaagccag ttt
//
LOCUS       DACERL010000044        23659 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.046, whole genome shotgun sequence.
ACCESSION   DACERL010000044 DACERL010000000
VERSION     DACERL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23659
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.046"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            257..532
                     /locus_tag="IXF54_001739"
     CDS             257..532
                     /locus_tag="IXF54_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1404996.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IXF54_001740"
     CDS             545..2623
                     /locus_tag="IXF54_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1404997.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IXF54_001741"
     CDS             2783..4669
                     /locus_tag="IXF54_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1404998.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IXF54_001742"
     CDS             4681..5628
                     /locus_tag="IXF54_001742"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1404999.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IXF54_001743"
     CDS             5648..6175
                     /locus_tag="IXF54_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1405000.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IXF54_001744"
     CDS             complement(6238..6891)
                     /locus_tag="IXF54_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1405001.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IXF54_001745"
     CDS             7023..7865
                     /locus_tag="IXF54_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1405002.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IXF54_001746"
     CDS             8023..8919
                     /locus_tag="IXF54_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1405003.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IXF54_001747"
     CDS             8922..9635
                     /locus_tag="IXF54_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1405004.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IXF54_001748"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IXF54_001748"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1405005.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IXF54_001749"
     CDS             10170..10397
                     /locus_tag="IXF54_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1405006.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IXF54_001750"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IXF54_001750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1405007.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IXF54_001751"
     CDS             complement(11183..11725)
                     /locus_tag="IXF54_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1405008.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="IXF54_001752"
     CDS             complement(11962..12360)
                     /locus_tag="IXF54_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1405009.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="IXF54_001753"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="IXF54_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1405010.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="IXF54_001754"
     CDS             13473..14240
                     /locus_tag="IXF54_001754"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1405011.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="IXF54_001755"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="IXF54_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1405012.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="IXF54_001756"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="IXF54_001756"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1405013.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="IXF54_001757"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="IXF54_001757"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1405014.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="IXF54_001758"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="IXF54_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1405015.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="IXF54_001759"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="IXF54_001759"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1405016.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="IXF54_001760"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="IXF54_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1405017.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="IXF54_001761"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="IXF54_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1405018.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="IXF54_001762"
     CDS             22691..23566
                     /locus_tag="IXF54_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1405019.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6405 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgattt
//
LOCUS       DACERL010000045        22766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.047, whole genome shotgun sequence.
ACCESSION   DACERL010000045 DACERL010000000
VERSION     DACERL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22766
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.047"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(28..1260)
                     /locus_tag="IXF54_001763"
     CDS             complement(28..1260)
                     /locus_tag="IXF54_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1405020.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="IXF54_001764"
     CDS             1514..2083
                     /locus_tag="IXF54_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1405021.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="IXF54_001765"
     CDS             complement(2261..2701)
                     /locus_tag="IXF54_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1405022.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="IXF54_001766"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="IXF54_001766"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1405023.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="IXF54_001767"
     CDS             3655..4578
                     /locus_tag="IXF54_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1405024.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4654..5793
                     /locus_tag="IXF54_001768"
     CDS             4654..5793
                     /locus_tag="IXF54_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1405025.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5786..6586
                     /locus_tag="IXF54_001769"
     CDS             5786..6586
                     /locus_tag="IXF54_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1405026.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6586..7413
                     /locus_tag="IXF54_001770"
     CDS             6586..7413
                     /locus_tag="IXF54_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1405027.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7391..8125
                     /locus_tag="IXF54_001771"
     CDS             7391..8125
                     /locus_tag="IXF54_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1405028.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8225..9091
                     /gene="rfbA"
                     /locus_tag="IXF54_001772"
     CDS             8225..9091
                     /gene="rfbA"
                     /locus_tag="IXF54_001772"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1405029.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9105..9677
                     /gene="rfbC"
                     /locus_tag="IXF54_001773"
     CDS             9105..9677
                     /gene="rfbC"
                     /locus_tag="IXF54_001773"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1405030.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9699..10727
                     /gene="rfbB"
                     /locus_tag="IXF54_001774"
     CDS             9699..10727
                     /gene="rfbB"
                     /locus_tag="IXF54_001774"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1405031.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10825..11676
                     /gene="rfbD"
                     /locus_tag="IXF54_001775"
     CDS             10825..11676
                     /gene="rfbD"
                     /locus_tag="IXF54_001775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1405032.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11710..13743
                     /locus_tag="IXF54_001776"
     CDS             11710..13743
                     /locus_tag="IXF54_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405033.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13787..15052
                     /locus_tag="IXF54_001777"
     CDS             13787..15052
                     /locus_tag="IXF54_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1405034.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNHNSYNSILNIYGLIGFGIAALLLILFIGRLTLKAD
                     LSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEEFYVME
                     EAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15262..16068
                     /locus_tag="IXF54_001778"
     CDS             15262..16068
                     /locus_tag="IXF54_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405035.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16080..17300
                     /locus_tag="IXF54_001779"
     CDS             16080..17300
                     /locus_tag="IXF54_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405036.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17290..18876
                     /locus_tag="IXF54_001780"
     CDS             17290..18876
                     /locus_tag="IXF54_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1405037.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFTDVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18915..21053
                     /locus_tag="IXF54_001781"
     CDS             18915..21053
                     /locus_tag="IXF54_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1405038.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21421..22404)
                     /locus_tag="IXF54_001782"
     CDS             complement(21421..22404)
                     /locus_tag="IXF54_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1405039.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7146 a         3888 c         4693 g         7039 t
ORIGIN      
        1 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
       61 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
      121 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
      181 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
      241 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
      301 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
      361 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
      421 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
      481 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
      541 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
      601 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
      661 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
      721 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
      781 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
      841 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
      901 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
      961 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
     1021 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
     1081 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
     1141 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
     1201 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
     1261 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
     1321 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
     1381 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
     1441 atcaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctaaag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttataa
     4621 taattatgac tcttaattaa agtaggtgcc catatgcttg tttcttttag tatggttata
     4681 agattttttc tgacaatttt attatcactg atattaacac ctgtattcaa aatcatttca
     4741 gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt tccaatgcct
     4801 tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt cttattccgt
     4861 aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt aatcatagtc
     4921 acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat agggatcttg
     4981 attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt cacgcttcct
     5041 tttattggat atatccaact aggctggctt agttttcccg taacgatttt ttggatcttc
     5101 ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc agggatttca
     5161 ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc ttctacagtg
     5221 tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt tccttacaat
     5281 ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg gtttatgatg
     5341 gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat ttctttgctg
     5401 atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag aaaagcaaac
     5461 cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat cagtttaggc
     5521 tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca
     5581 gctatggtca tgaattatac tggaagaatc ggaacgatca tattgattat tgccatgttg
     5641 tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca tcagtttatt
     5701 ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa acagaaatat
     5761 ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga tccttattag
     5821 tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta tttcaactgt
     5881 aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc ccgagtacaa
     5941 aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc atgatgaaaa
     6001 ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc gctatggcat
     6061 tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg tcttgttatt
     6121 gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag atggaacaac
     6181 tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct ttgtgccttt
     6241 caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc gaggattgcc
     6301 tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag ctgatattga
     6361 taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg aagacaacga
     6421 cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct tttatgcagt
     6481 tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac
     6541 ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa accagaaata
     6601 ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta tggaccatta
     6661 agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag tcctcaaagg
     6721 cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct
     6781 ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac acgttgtctc
     6841 gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt gcgtaaagta
     6901 acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag ccgacaagtt
     6961 tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga agttgaaggc
     7021 ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt caaatttcta
     7081 aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca ttggctgttt
     7141 tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat ggaacatgat
     7201 ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat tttacaagca
     7261 gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg acgacaatta
     7321 ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg gcggatgact
     7381 ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt gtatagctac
     7441 ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc agctttcagc
     7501 agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg atatattaac
     7561 tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc aaggattgat
     7621 cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt tggcagacca
     7681 agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg agcagaatcc
     7741 ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc aagagatcca
     7801 tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg tgataagaag
     7861 tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata ttttgccgat
     7921 cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa agaagactgg
     7981 gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga gtgagattga
     8041 aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta cggcgttgat
     8101 caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat ttccatgaca
     8161 aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag gagagcagat
     8221 atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa
     8281 ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt atccaatgtc
     8341 tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac aagatacacc
     8401 acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg aatatgcagt
     8461 acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat ttattggaga
     8521 tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc tgtctaaaat
     8581 gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc atgtcaatga
     8641 tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt cgattgaaga
     8701 aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt atgacaatga
     8761 ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag agattacgga
     8821 tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg gacgcggatt
     8881 tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct ttatcgaaac
     8941 aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt accgtatggg
     9001 ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg
     9061 tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa gtaacagata
     9121 caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat cgcggtttct
     9181 tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat gactttgttc
     9241 aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag
     9301 gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta gatgtagtag
     9361 tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc ttatctgaac
     9421 ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt actttgacac
     9481 caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca gatgggggca
     9541 tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca agagcgatta
     9601 tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa aatccatttg
     9661 tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc attgttaccg
     9721 gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat catccagaag
     9781 tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac ttggctggac
     9841 ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa ttagtggacc
     9901 gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat aacgataatt
     9961 cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat acacttttag
    10021 aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag gtttacggcg
    10081 atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa aaattcactg
    10141 cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt tcagatctgt
    10201 tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac tgttcaaaca
    10261 actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg aatatcttaa
    10321 gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg atccatacga
    10381 atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc gaaacctatc
    10441 tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc ttagaaatga
    10501 tgggtcaacc aaaagatgcg tacgaccatg taaacgaccg tgcaggacac gacttgcgtt
    10561 atgcaatcga ttcgacaaaa ttacgggaag agttaggatg gaagcctgag tttacgaact
    10621 tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac tggtggaaaa
    10681 aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac agagaaaatg
    10741 tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct tttttaaaat
    10801 aaaaatcaag gagcggataa tcatatgttt ttattaactg gaggaaatgg gcaactaggt
    10861 acagaattgc gccatttatt agatgaaaaa aatctgcaat atgtttcaac agatgcaaaa
    10921 gacttggata tcacagatgc cgagaaaaca atggcttaca tcacagaatt aaaacctgat
    10981 gttattttcc attgtgcagc ttacacagca gtagataaag cagaagaaga agcaaaagaa
    11041 ttagatgaga agatcaatgt tgatggtaca agaaatgtag cacttgcagc aaaagctgtg
    11101 aatgctcaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa caaaaatgta
    11161 gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac gaaattatta
    11221 ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac ttcttgggtt
    11281 ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga aacccgcgac
    11341 aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac tttggcagaa
    11401 tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt gtcgaacgaa
    11461 aatagctgtt cttggtatga attcgcaaaa gaaatattga aagatacaga tgtagaagtt
    11521 gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta ttcagtcatg
    11581 agcctgaaaa agacagaaga gttaggattt gtcataccaa cttggcaaga agcacttgcg
    11641 caaatgctgg aagctgccaa aaaaatgaat aagtaatttc cttttatcaa atagaatttg
    11701 gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaac
    11761 caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt
    11821 ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat
    11881 aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg
    11941 gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag
    12001 gactttccgt tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat
    12061 aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc
    12121 ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg
    12181 cttggattcg aagtactgat gatattaacg aagagaaaaa aagctctatc gctaatcggt
    12241 gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat
    12301 ttgattttct ttactttggg actgatgact gctttttatc attatttcta caatcatgat
    12361 aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt
    12421 ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt
    12481 ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt
    12541 ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac
    12601 gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag
    12661 tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc
    12721 aactattcga ataactctga agtggccctg ttctatcact tttttccaac tgtattccta
    12781 gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt
    12841 ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta
    12901 gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt
    12961 gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct
    13021 atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg
    13081 cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga
    13141 acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct cgcttttgcc
    13201 gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt
    13261 tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt
    13321 tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat
    13381 acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa agaagaatac
    13441 gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag
    13501 ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa
    13561 ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa
    13621 gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa
    13681 tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag
    13741 taataggcta gctgagaata atcctgaaat gttagtagaa aggagagtgg agataattca
    13801 agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttactgac
    13861 gctagtcttt tttgtttgga cagcagggtt aggttttcca gcaactttgt ttatatataa
    13921 caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa
    13981 agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc
    14041 ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc ctttgattat
    14101 tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct
    14161 gattgtcttt tcggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt
    14221 accagaagga caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac
    14281 gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc
    14341 ttttgaacga tggtatgtac gaatcatcgg tgcagtagca gttgttgcag cttttctcgg
    14401 gatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga
    14461 tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgctt acacggttac
    14521 gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt
    14581 taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca
    14641 aattttacta ggaatgaaaa catttatctt ccaacgagga aaccacaata gttataattc
    14701 tattttgaat atctatggat tgataggatt tgggattgct gctttgctgc ttatcctttt
    14761 tattggtcgc ttaacgttga aagcagattt atccaacggg cagatgactt ttatctgggc
    14821 cttttttgcg gtgatgatgc agtcatttat ggaggataca ttgacttcat acccatgggt
    14881 tccgattatt tatatcctgt tggctatggc cgttcatcga tatgatgaac cagaagttgc
    14941 ggaggaattc tatgttatgg aagaggcata tgacgatatg acttatgatg atacatctta
    15001 tgaggaacaa ccagatagcg ctcattcttc caggatccaa cgctatcatt gataggcaca
    15061 aaatacgaga gaggctgtga caaccgtcgc agcctttttt ggtttattgc aattgtctag
    15121 catcttctgt aaaatagaaa cgttagagaa aatcttgtat catagaaaat taagaaagaa
    15181 ataagaacgc agctcggtac caaaaatatg gtaaaatggc acttgactgt aattcactga
    15241 aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt tatttatgaa ggacatttat
    15301 cagaacagaa aattattgct acagttttcg atcaatgact tcaaatcaag atatgcagga
    15361 tcgtttttag gaatcttatg ggcgtttatc aatccgctat ttactgtatt gatttattgg
    15421 ctggtatttg gtgttggact gaaagcacaa atgatcgatg caaaataccc atttatcgta
    15481 tatttagtta ccgggatgat tccctggttc ttcttctcag atgcatttgc atcaactacg
    15541 gttgtttttc gggaatatac ttatttagta aagaaagtcg tattcaatat tcgtatcttg
    15601 cccacgacga aaatcctatc caatctgtac acccatttat tcttcattct gattggcata
    15661 gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt ctttacagct gatttattat
    15721 ttattttgca tgtgtgcttt cttgacagga ttgacttggc tgacggcatc gatccaacca
    15781 tttttacctg atatcatgca attaattacg atcgtgctgc agttgatcat gtggacatta
    15841 ccgatattat ggagccctaa tcagtttgat tcaggtcttg taagcatatt gaaattaaat
    15901 ccgctgtact atgttgttca aggttatcgt gagtcatttt taagtgaagc ttggttctgg
    15961 gagcatggaa catatacttt gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc
    16021 tctgcagtgt tccgtagatt gaaaccacat ttttcagatg tgctgtaagg agaatagcta
    16081 tgacagaata tgcaattaga ctaaaagata tcacaaaatc ttataatatg tacgcaaaac
    16141 cttcggatcg atttcgtgaa gccctcaatc cttttaagaa atcctatcat gatgtatttt
    16201 atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag
    16261 aaaacggatc gggaaaatct acaatgttga aaatcattac gggagtactg acaccaacat
    16321 ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct agaactagga tcaggattca
    16381 atcctgagta ttctggctat gaaaatatct atttaaatgg aatggttcta ggattttcaa
    16441 gagaagaagt cgatgaaatg gtcgatgata tcattgaatt tgctgacatc ggagaccata
    16501 tttatcaacc tgtaaaaact tattcaagcg ggatgttcgt tcgtctagct tttgctgtag
    16561 caatcaatgt gaatcccgat attttgattg tggatgaagc attggcagta ggcgatcttg
    16621 aatttcaact aaaatgtatg gaaaaattta cagaaatcaa gaactcggga aagacgattt
    16681 tattcgtttc tcatgatatc agttcgattc gtcgtttttg tgatcgttcc tattggttga
    16741 aaaacggaga agtcgtcgaa tatggcgata cgatggaagt aacgacgaat tatgaaaact
    16801 ttttaaagaa aaaatcagtt aaaacagtgg atcggaataa aaaccaagta gaacggttcg
    16861 ccgctccaga tatcgtcgac gtgacgaaag cggaattgtt ggataaagac aaaaatccgg
    16921 tggatatgct ggaacaagga gaaaaactat acgttaaagt aacttatgaa gtaaaagatg
    16981 atacaatcaa aaatccggtt ttaggaattg cgctacgaac agtcgacaat ttttatgtat
    17041 gcggacttaa tactctacta gatggtatca agattccttg gaaaaaagga gagaatatct
    17101 tttatctgga gtatgaaaag ttaggtttgt tagctggtga atattatttt gacgttgcgg
    17161 tctttgaaga aaatgcaacg gttcctttgg tatataagac aaagtacatg aacttgtttg
    17221 tcagcggttc ttatatcgga gaaggcatcg tggtcttaga ccataaatgg gaagaaggga
    17281 ctcatagcaa tgaaatatga ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa
    17341 attgccgcac agatcaaacc aggaagtaaa gttttagagt ttggtcccgg aaacggccga
    17401 ttaacgaaac atttaatcgg agcaaaacaa tgtgaagtga gcattgttga gttagataag
    17461 gaattgttcg attttgtcag cgaatttgct caagatggat tttatggtga tatcgaaagt
    17521 tttgagtggg caaactatta tgctggacag acatttgatt acgtcttgtt tacggatgtt
    17581 ttagaacatt tggtagatcc tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa
    17641 gaaggagaga tcctgattac attccctaac ttggcgcata attctgtcat gatcgatttg
    17701 ttcaataacc aattgccgtg ggcttcttat ggtctcctag atgaaaccca taattctttt
    17761 tatactcatg atggcttcca aaaagttttt gaaaaagcgg gattattcat taatattgaa
    17821 gattatttgt acttagctgt aggtgataca gaattaaaat caacttatga agaattgccg
    17881 gaagctgtac gctatgattt caaaatgcga ccatttggtg aagtctacca atactttttc
    17941 tcattgatga aacatccagt agcacaaagt tccattgcag agccacaaaa ttctaattat
    18001 gtaaaagtgt tagaagtaac ccagcaaacg aaacaagatg aaacaatcca acaaatacca
    18061 ttcaataatt tcacagggga aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa
    18121 agaatggtgt ttcgcttcgc gcaacaacct agctttatcg aattcagcgc agaagctgcc
    18181 ggagaaaaac taacatttat cgattcgaat gctgtagtca aaacagtaaa tgattgttat
    18241 ttgtttgatg ggaaagaatt accagagttc gttctaacgg atatttctgg gaaagaagtg
    18301 actatccatt gtcactatcg ctttatcggg gaattgacac caacaatgaa agaattgttg
    18361 gaaacgattc gtcccatggc tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta
    18421 gaaagagaaa atcatcatct tcttgaggaa aatacaagac tagaccatac gttaaaaaca
    18481 accacaaatc gctattgcac attgttggaa agtgatgaat ggacaataaa acatagacta
    18541 ggacgacgta agaaagaaac ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt
    18601 gacgagaaaa tctgggatgc agaaacaaaa atattaaaaa tcatcggatg gggaatcgct
    18661 aattcggatc gccagccctt gtcctataag ttatcagctg accaatcgcc atttttcgaa
    18721 gcaattcctg tttcacgaga agaagtcaat caagcagaac agctcgcaaa aggaacagaa
    18781 gcgggatttg agttacgaat cctttgcgag caagaacgtt cattcttagt ggaagcagta
    18841 gctcaaaatg gccaatcatg gatcattgaa atgtaaataa tgattaactt ataaagataa
    18901 ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg
    18961 aacgggccac tggcgatttg cttattgtgg gctgggcaat tgatgaagtg accaaagaaa
    19021 tccctacgat aaaggtagaa aaagaaaatg tgattgctga agcaacacat gtcgtaagat
    19081 tggatatcaa tcatttatat aatcttgatg ttaagaccca aagcggtttt aaggtacgtt
    19141 tatcagggaa aatgagagga aaagccatct tagacttcca aacagcgaga catcaaaatg
    19201 gcatagctgt gaaattaaac ggtaaatacc cttatgatga cggcattgaa tcttcatggg
    19261 aaagaaaaaa aagattactg aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa
    19321 aagcgatccg tcgggtcaaa ttggaattga agccaggatc gatcgactat gcagagtgga
    19381 tcagtcgtca tgagaaacca gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt
    19441 accgtccatt gatttccata cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa
    19501 aatgcattga ttcggtactg gatcaaacgt atgatcattg ggaactatgt atcagtgatg
    19561 atgcttctac tgatccagct atccgaaagt gtctggaatc ctatcaagca aaagatgata
    19621 gaatcaaagt cgttttccga aaagaaaatg gacacatcag tctggcaact aattctgcat
    19681 tggaaatagc agaaggagaa tttatagcac tcttagataa tgatgatgaa ttaccgccat
    19741 ttgcattata tgaagtggca aaagtgctaa acgtacatcc agaactggat gtgatctaca
    19801 gtgacgaaga taaaatcgat gcagatggca atcgctttga tccgcatttc aaagcagact
    19861 ggtcgcctga tacattgatg ggaaataatt atatctctca cttaggcgta tatcgaacaa
    19921 gcattgttaa agaacttggc ggtttccgta aaggctatga aggttctcaa gattatgatt
    19981 tggtccttcg tgtgacagaa caaatccctg cagatcatat ttaccacatt gaccgagtct
    20041 tgtatcattg gcggacgatc ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata
    20101 tctatgattc cggagtgaaa gcattgacag atgccctgtc tagaagaaac atcaaaggaa
    20161 gcgtacgtcc tggcagaatt tctggatttt atgagattgc ttatgacgtc ttgcaagagg
    20221 atttggtcag tgtgatcatt ccaacgaaga atggctatga agatctaaaa acttgtgtgg
    20281 attcgatcat cgaaaaaacg tcttattcaa attatgaaat cattattgcg gataacggaa
    20341 gtacagatcc aaaaatgcaa gaattatttg cagaatacaa gcatcagctg aaagaccgct
    20401 ttatcgtaga attgattgac attccattca attattctcg aattaacaat ttagcagcag
    20461 aaaaagcaaa cggcaaatac tttttattct tgaataacga tacagaagtg attgagccag
    20521 attggatgac ggtgatggtt tcttatgcac agtttgatcg aatcggctgt gtaggagcaa
    20581 aactgtttta tccagatgat acaacgcagc atgcaggagt attattagga atcggtggtg
    20641 ttgcagggca tgcattgaat aactatgatc gaacacattg tggttatttc ggccgattgg
    20701 tcattgacgt caattactta gcagtcactg cagcttgtat gatggtcaag gcagctgact
    20761 tcaatgctgt taacgggttt gatgaaacat tagaagtagc attcaacgat gttgacttgt
    20821 gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc acatcaagca gaattgtacc
    20881 atttcgaatc taaatcaaga ggttatgaag atactccaga aaaacaaaga cgttttgctg
    20941 gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat tgcgcatgat ccattttaca
    21001 atgataattt aacaaaagaa ggaatcggcg acttttcgtt gagaccagat taaactataa
    21061 taaaaagctg ttatgttgtg attcgctcgc acataacggc tttttatagt tttttgtcaa
    21121 aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac
    21181 tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcggctt
    21241 atttaagaag aagttgcttc tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa
    21301 gtgtttctta cttttgtccc acgctcttat tcatgctcag agcgccaata gatacaaaat
    21361 catagccaaa gtttcacgaa ccctagctgt caaagaatcg atattttatt ttaatatagg
    21421 ctattttaaa ataaatggtt taaggtattc ctctacaaaa gccactctat caattatcat
    21481 gggtttaaaa taagaagtga tggagataac cagattgttc ttagcgtcaa tataaagaac
    21541 atttccacta ttaccaattg ctgaatatat ttcgttctca gaatctaata cccaccataa
    21601 atagccatat ctcatatttt gtaagtcttc taaagcactg gttctaggtt tgaacatctc
    21661 gtttatccac atgggtggta cgatttgttt tcctttatac atccctttat ttaggcataa
    21721 tacaccaatt tttgccagat cacttgccga taaacacaaa ccatatcctg ccgtcccgat
    21781 atttagtgga tcacaaaacc agacattggt tttaggagac ttatccattg taaactgctt
    21841 atgttcttca gctgtttcag cataataatt gacatggttt ggtatcccta taggatcaaa
    21901 tagatactta ttggcaaaat caacagttgt tagcttactt gtctgataca acaagccagt
    21961 taatatgtgt aaacagacag tttgatagtt gaattcgccg gttatacctt ttcttcctcc
    22021 caataaatct agtgatgttt ttgtccaatc ttcacttgaa cagacttttg tccatggatc
    22081 gcctttacat ttgtagggtg ctttcattgt tagcaagtgc ttgattgtaa tagcgtaaat
    22141 agttttctcc ccgcgtttca ctttatactc tggaaaataa tctaatactt tatcatcgat
    22201 acttttgatt aatccttgct ctaaagcaat gccaatcagc aaagatacaa tacttttcgt
    22261 tgctgacatg atatgacaag tatcgtcttt tttataacca ttccattcat ctgtataaac
    22321 ttctacccca tccttatatg cgacaatttg acatatattt ggttgtcgaa aaccaataaa
    22381 atcatgcaac tcttgtttat tcataagata ttacctcact ttcttctata agcataatgt
    22441 cattaaaaat cgatttcaat tttttcgatt cttcacgtaa tacaggtatt cttatggact
    22501 agacagcgct ttgggaagat ggattctctg aaaaatataa aggttcactt tatgagtgga
    22561 taaaaaaagc agcaaatact tcgacttctt taatttacca acattagtga aaatcgattc
    22621 atctggagat ttgtaattta gagtccttgt cttgattaga ggtgtataaa gctttttatt
    22681 agacattttt aaattccatt acaatttgct tataaactag gtttttaaag gtgactatgg
    22741 taagataata gagatattat tttgtg
//
LOCUS       DACERL010000046        22553 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.048, whole genome shotgun sequence.
ACCESSION   DACERL010000046 DACERL010000000
VERSION     DACERL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22553
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.048"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(133..306)
                     /locus_tag="IXF54_001783"
     CDS             complement(133..306)
                     /locus_tag="IXF54_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1405040.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IXF54_001784"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IXF54_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IXF54_001785"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IXF54_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1405041.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IXF54_001786"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IXF54_001786"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1405042.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IXF54_001787"
     CDS             5704..6489
                     /locus_tag="IXF54_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1405043.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IXF54_001788"
     CDS             6502..7353
                     /locus_tag="IXF54_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1405044.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IXF54_001789"
     CDS             complement(7477..7929)
                     /locus_tag="IXF54_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405045.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IXF54_001790"
     CDS             complement(8394..8762)
                     /locus_tag="IXF54_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1405046.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IXF54_001791"
     CDS             8912..9394
                     /locus_tag="IXF54_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1405047.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IXF54_001792"
     CDS             9431..10441
                     /locus_tag="IXF54_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1405048.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IXF54_001793"
     CDS             complement(10556..11269)
                     /locus_tag="IXF54_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405049.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IXF54_001794"
     CDS             complement(11459..12049)
                     /locus_tag="IXF54_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1405050.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IXF54_001795"
     CDS             12533..12760
                     /locus_tag="IXF54_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1405051.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13941
                     /locus_tag="IXF54_001796"
                     /pseudo
     CDS             12776..13941
                     /locus_tag="IXF54_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            14175..15218
                     /gene="hrcA"
                     /locus_tag="IXF54_001797"
     CDS             14175..15218
                     /gene="hrcA"
                     /locus_tag="IXF54_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1405052.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15312..15875
                     /gene="grpE"
                     /locus_tag="IXF54_001798"
     CDS             15312..15875
                     /gene="grpE"
                     /locus_tag="IXF54_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1405053.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15926..17755
                     /gene="dnaK"
                     /locus_tag="IXF54_001799"
     CDS             15926..17755
                     /gene="dnaK"
                     /locus_tag="IXF54_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1405054.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGHVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17906..19072
                     /gene="dnaJ"
                     /locus_tag="IXF54_001800"
     CDS             17906..19072
                     /gene="dnaJ"
                     /locus_tag="IXF54_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1405055.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19154..19819)
                     /locus_tag="IXF54_001801"
     CDS             complement(19154..19819)
                     /locus_tag="IXF54_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1405056.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19795..19968
                     /locus_tag="IXF54_001802"
     CDS             19795..19968
                     /locus_tag="IXF54_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1405057.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20196..20402)
                     /locus_tag="IXF54_001803"
     CDS             complement(20196..20402)
                     /locus_tag="IXF54_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405058.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(20428..20847)
                     /locus_tag="IXF54_001804"
     CDS             complement(20428..20847)
                     /locus_tag="IXF54_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1405059.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            21351..21704
                     /locus_tag="IXF54_001805"
     CDS             21351..21704
                     /locus_tag="IXF54_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405060.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(21764..21931)
                     /locus_tag="IXF54_001806"
     CDS             complement(21764..21931)
                     /locus_tag="IXF54_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1405061.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(22050..>22553)
                     /locus_tag="IXF54_001807"
     CDS             complement(22050..>22553)
                     /locus_tag="IXF54_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405062.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
BASE COUNT         7582 a         3870 c         4594 g         6507 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
    13801 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
    13861 gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag
    13921 cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa
    13981 aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat
    14041 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
    14101 gagaagggct tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat
    14161 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
    14221 actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg
    14281 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
    14341 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
    14401 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
    14461 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
    14521 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
    14581 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
    14641 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
    14701 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
    14761 ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc
    14821 agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa
    14881 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
    14941 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
    15001 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
    15061 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
    15121 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
    15181 ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
    15241 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
    15301 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
    15361 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
    15421 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
    15481 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
    15541 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
    15601 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
    15661 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
    15721 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
    15781 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
    15841 tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt
    15901 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
    15961 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
    16021 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
    16081 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
    16141 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
    16201 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
    16261 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
    16321 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
    16381 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
    16441 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
    16501 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
    16561 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
    16621 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
    16681 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
    16741 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
    16801 tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg
    16861 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
    16921 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
    16981 gatcactggt catgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
    17041 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
    17101 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
    17161 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
    17221 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
    17281 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
    17341 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc
    17401 aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc
    17461 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
    17521 agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat
    17581 cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
    17641 actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg
    17701 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
    17761 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
    17821 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
    17881 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
    17941 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    18001 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    18061 agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc
    18121 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    18181 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    18241 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    18301 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    18361 aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    18421 taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat
    18481 gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc
    18541 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    18601 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    18661 cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg
    18721 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    18781 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    18841 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    18901 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    18961 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    19021 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    19081 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg ttcgcctggt
    19141 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
    19201 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
    19261 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
    19321 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
    19381 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
    19441 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
    19501 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
    19561 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
    19621 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
    19681 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
    19741 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
    19801 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
    19861 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
    19921 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
    19981 ccttcatggc gaccgtgcat ttttggtttt tattaaattt tatatctaat tttcaattat
    20041 ccgcataaac actatacatt caagcatttt gaagtataga aaaacactca tcttaccacg
    20101 aatttaccac gattgaatga gccttgatat gaccgtaaaa ctactaaggg agataacacg
    20161 aaactgtgta tctcccgatt tttttataca gttgattact tcactccgat ttcaaatttc
    20221 aatattgctt tcatcatgtc tgtatgaatt tctcctttta gttcttcatc aacctgcata
    20281 atgattttac cactttcaga gtggtaaaaa ggacgcaagc ataatttaga aatataaccg
    20341 tcataaaact tcaaaatctc tttaactgcc atttcatctc cgcttgacgc tttgcaaatc
    20401 aattcaaaag agggatagcc ataatcttta cttcctttcg ctcaaaagca tttttaactt
    20461 ccttagtcct ctagttcttc tactgcaaat agcagaacgt gatacatggt acagttctga
    20521 aatttctctg tcgttcattc cttgaaaata acgtaataaa atcgcactac gctgtttttc
    20581 tgatagtcta tgtaaaactg taccaagtgt ttcatcagat acataaattg taaagtgcaa
    20641 gactttaaat gatacacaat cataaaaaca ggtatcaatc gttccacgtt caacttgttt
    20701 cgtttcggct atttcacaaa acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt
    20761 tcgacttttc actttgttct taatgacaat cagcattaaa gaattgaact gtaatctgac
    20821 gatatgctcg aaagaagatg tcctcataat ctcaccccct ttctgtctag gaaaggaagt
    20881 gagccttgct cgtttatctc ctttcactct tagtcccaat gtgaaagggg gatttgttgc
    20941 attactgata aataaacttt gtaaaaaagt tctgaatagc caaaaaagca tataaacaga
    21001 tttatttctc tgtttacatg cttctgttat tctatctata tgatttataa aaccacattg
    21061 gtggacgtac ttatctattg cagatagacg actttttttg acaagaaccc aatgtaagga
    21121 aatttattgt atatgatgta cttcatggcg acgttgacct ccaacaaacc gccatttgga
    21181 agtaatatac aatattttaa cagcgtaaat agcactacca tataacggtt ttttttattg
    21241 gcgtttagta gtgcttttta ttaaatataa acctataaac catataacac gtttttctat
    21301 acctgttttt aattcagtag gaacaataaa atgtatagag gtggtctact atgcgtaaaa
    21361 aagaagataa atatgatttt agagcctttg gtttagccat taaagaagct cgattgaaac
    21421 gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat tgacccacgg tacttaacta
    21481 atattgaaaa taaagggcaa caccccagca tacaagttct ttatgacctt gtatcgttac
    21541 ttcatgtttc cgttgatgaa tttttcttac ctgctaataa cttggtaaaa agcacccgac
    21601 gattacagat agagaaatac atggatagct ttacagacaa agaactatcc ttaatggaat
    21661 ctttagccag cggtatcaac gaagcaagaa acatcgaaga ctaattaaaa gaatccatac
    21721 ataacggaaa gagccgataa aatgagattg tattaatctc attttatcgg ctctgcgtct
    21781 ttgcgtctgg ctctgtaatc acagttactt tgaactgctt tatttcaatt aaattttctt
    21841 gtctgcattt cggacaatag agggggaatt tttttaattc agtatcttcc cttatcttta
    21901 atcgtgtttt atttccacat acaggacaca atatccactt gtagtttata ataactatct
    21961 cctcctttac actttaattc aaatctttat taaaaaatat ttcatcttat ttaacaagaa
    22021 accatattta tataacaaca taaaatacac taagttattt tattgaacat atatcgtact
    22081 ttatctatcc gactatttgg acgacggggc tggcaaacag gttcaccggt agtaacatgg
    22141 taccctttta actctgttaa acaaacacta cgtccatttg taaagaaagt taaatcacta
    22201 cgatattctt gaatacaccg agcagggatt tctccactaa gaatgacctc attatttttc
    22261 aattgagtgt ctacgatgtt cgcacaatat ttaggagcat cgttgtatgc tcgtgaaaga
    22321 tattcctgtg gcgcataaat tttaaaacta agatatggct ctaacaattc tgttccagct
    22381 ttttttaaga cttgttccaa tacaatagga gcaagcatcc gaaaatctgc tggggtacta
    22441 acagggctat agtataagcc atacttaaaa cagattttac agtccgtcac attccaacca
    22501 tacaatcctt gttcacagcc atagcgtatc ccctccataa ctgcattttg aaa
//
LOCUS       DACERL010000047        21923 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.049, whole genome shotgun sequence.
ACCESSION   DACERL010000047 DACERL010000000
VERSION     DACERL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.049"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            282..521
                     /locus_tag="IXF54_001808"
     CDS             282..521
                     /locus_tag="IXF54_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405063.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IXF54_001809"
     CDS             complement(883..1143)
                     /locus_tag="IXF54_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405064.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IXF54_001810"
     CDS             complement(1328..1825)
                     /locus_tag="IXF54_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1405065.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXF54_001811"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXF54_001811"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1405066.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXF54_001812"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXF54_001812"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1405067.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IXF54_001813"
     CDS             complement(4547..5209)
                     /locus_tag="IXF54_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1405068.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IXF54_001814"
     CDS             complement(5219..6034)
                     /locus_tag="IXF54_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1405069.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IXF54_001815"
     CDS             complement(6296..6739)
                     /locus_tag="IXF54_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1405070.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IXF54_001816"
     CDS             complement(6873..7211)
                     /locus_tag="IXF54_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1405071.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IXF54_001817"
     CDS             complement(7199..7576)
                     /locus_tag="IXF54_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1405072.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IXF54_001818"
     CDS             7800..8993
                     /locus_tag="IXF54_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1405073.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IXF54_001819"
     CDS             complement(9159..9581)
                     /locus_tag="IXF54_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1405074.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IXF54_001820"
     CDS             9976..10170
                     /locus_tag="IXF54_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405075.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="IXF54_001821"
     CDS             10167..10661
                     /locus_tag="IXF54_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405076.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="IXF54_001822"
     CDS             complement(10823..11995)
                     /locus_tag="IXF54_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1405077.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="IXF54_001823"
     CDS             complement(12201..13016)
                     /locus_tag="IXF54_001823"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1405078.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="IXF54_001824"
     CDS             complement(13166..13612)
                     /locus_tag="IXF54_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1405079.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="IXF54_001825"
     CDS             complement(13609..14613)
                     /locus_tag="IXF54_001825"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1405080.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="IXF54_001826"
     CDS             complement(14653..14982)
                     /locus_tag="IXF54_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1405081.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="IXF54_001827"
     CDS             complement(15108..17435)
                     /locus_tag="IXF54_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1405082.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18278..18571)
                     /locus_tag="IXF54_001828"
     CDS             complement(18278..18571)
                     /locus_tag="IXF54_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1405083.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18974..20152)
                     /locus_tag="IXF54_001829"
     CDS             complement(18974..20152)
                     /locus_tag="IXF54_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405084.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20256..20570)
                     /locus_tag="IXF54_001830"
     CDS             complement(20256..20570)
                     /locus_tag="IXF54_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405085.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20677..20892
                     /locus_tag="IXF54_001831"
     CDS             20677..20892
                     /locus_tag="IXF54_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405086.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21192..21434)
                     /locus_tag="IXF54_001832"
     CDS             complement(21192..21434)
                     /locus_tag="IXF54_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405087.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21421..21849)
                     /locus_tag="IXF54_001833"
     CDS             complement(21421..21849)
                     /locus_tag="IXF54_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1405088.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4313 c         3706 g         7450 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
    17581 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
    17641 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
    17701 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
    17761 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
    17821 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
    17881 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
    17941 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
    18001 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
    18061 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
    18121 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
    18181 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
    18241 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
    18301 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
    18361 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
    18421 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
    18481 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
    18541 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
    18601 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
    18661 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
    18721 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
    18781 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
    18841 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
    18901 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
    18961 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
    19021 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
    19081 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
    19141 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
    19201 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
    19261 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
    19321 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
    19381 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
    19441 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
    19501 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
    19561 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
    19621 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
    19681 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
    19741 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
    19801 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
    19861 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
    19921 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
    19981 tgcttgcaac aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt
    20041 gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg
    20101 tgatcctacc agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat
    20161 tcctcctatt ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt
    20221 catcattgta ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat
    20281 ttttctcgat agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa
    20341 tagtgtcctc gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc
    20401 agtgtactat cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt
    20461 aaatgactga ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa
    20521 tagaactcta tttccagaga attaacattt gttttaactt ccaactccat cacccttctc
    20581 tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac
    20641 tccatcaaaa cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata
    20701 aaaataatta aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga
    20761 atcaacttga gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat
    20821 cttgccgatg ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa
    20881 atatggaaat agtagttttt ctgatctatc agctgttttg ccaaagcaca taggaatctc
    20941 acctcccccc agtaatggcg ggccgatttg gttacggaag tatcattatc gtagtcgcga
    21001 ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc atggcgtaac tagagaatca
    21061 aaatctacat gtactgatag atttgctatt catttgtcaa agagcgctag gctattttca
    21121 gcctatggaa atcctttatt ttgacccgat aaaaaacttc catagaatga aaaggaaagg
    21181 gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct tctcaatcaa ttttgtttcc
    21241 agttgtctac gcatatattc atctacatac ataaccattc ctttttcttc ttcataaact
    21301 ggtttcatgg ctaaccgtga aatataattt tgatattttg acagaacgat ggacatggca
    21361 attgaatcac cagaagctgc ggctttgatt gtcgttagtg gtatgaatgg tatatatcgt
    21421 ttattttgct tcatatttga cacccaaata ctttcctagc tcttccaaag ccatttgtcg
    21481 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
    21541 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
    21601 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
    21661 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
    21721 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
    21781 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
    21841 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
    21901 tccattatta ctacaatcta gga
//
LOCUS       DACERL010000048        21561 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.050, whole genome shotgun sequence.
ACCESSION   DACERL010000048 DACERL010000000
VERSION     DACERL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21561
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.050"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            61..459
                     /locus_tag="IXF54_001834"
     CDS             61..459
                     /locus_tag="IXF54_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1405089.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            443..1138
                     /locus_tag="IXF54_001835"
     CDS             443..1138
                     /locus_tag="IXF54_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1405090.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1722..2609)
                     /locus_tag="IXF54_001836"
     CDS             complement(1722..2609)
                     /locus_tag="IXF54_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1405091.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2794..3633
                     /locus_tag="IXF54_001837"
     CDS             2794..3633
                     /locus_tag="IXF54_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1405092.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3829..4803
                     /gene="sgrA"
                     /locus_tag="IXF54_001838"
     CDS             3829..4803
                     /gene="sgrA"
                     /locus_tag="IXF54_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1405093.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5101..5457
                     /locus_tag="IXF54_001839"
     CDS             5101..5457
                     /locus_tag="IXF54_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405094.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5460..6617
                     /locus_tag="IXF54_001840"
     CDS             5460..6617
                     /locus_tag="IXF54_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405095.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6643..7083)
                     /locus_tag="IXF54_001841"
     CDS             complement(6643..7083)
                     /locus_tag="IXF54_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1405096.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7243..7749
                     /locus_tag="IXF54_001842"
     CDS             7243..7749
                     /locus_tag="IXF54_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1405097.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7917..9830
                     /locus_tag="IXF54_001843"
     CDS             7917..9830
                     /locus_tag="IXF54_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1405098.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9835..10836
                     /locus_tag="IXF54_001844"
     CDS             9835..10836
                     /locus_tag="IXF54_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1405099.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11038..11244)
                     /locus_tag="IXF54_001845"
     CDS             complement(11038..11244)
                     /locus_tag="IXF54_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1405100.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11890..12738)
                     /locus_tag="IXF54_001846"
     CDS             complement(11890..12738)
                     /locus_tag="IXF54_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1405101.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12902..13804
                     /gene="gnd"
                     /locus_tag="IXF54_001847"
     CDS             12902..13804
                     /gene="gnd"
                     /locus_tag="IXF54_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1405102.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13806..14807
                     /locus_tag="IXF54_001848"
     CDS             13806..14807
                     /locus_tag="IXF54_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1405103.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14804..15088
                     /locus_tag="IXF54_001849"
     CDS             14804..15088
                     /locus_tag="IXF54_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1405104.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15121..15918
                     /locus_tag="IXF54_001850"
     CDS             15121..15918
                     /locus_tag="IXF54_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1405105.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15932..16765
                     /locus_tag="IXF54_001851"
     CDS             15932..16765
                     /locus_tag="IXF54_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405106.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16783..17640
                     /locus_tag="IXF54_001852"
     CDS             16783..17640
                     /locus_tag="IXF54_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1405107.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17662..18171
                     /locus_tag="IXF54_001853"
     CDS             17662..18171
                     /locus_tag="IXF54_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405108.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18208..18609
                     /locus_tag="IXF54_001854"
     CDS             18208..18609
                     /locus_tag="IXF54_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405109.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18721..19074
                     /locus_tag="IXF54_001855"
     CDS             18721..19074
                     /locus_tag="IXF54_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405110.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19108..19551
                     /locus_tag="IXF54_001856"
     CDS             19108..19551
                     /locus_tag="IXF54_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1405111.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19568..20653
                     /gene="uxuA"
                     /locus_tag="IXF54_001857"
     CDS             19568..20653
                     /gene="uxuA"
                     /locus_tag="IXF54_001857"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1405112.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20646..21359
                     /locus_tag="IXF54_001858"
     CDS             20646..21359
                     /locus_tag="IXF54_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1405113.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7426 a         3308 c         4500 g         6327 t
ORIGIN      
        1 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaacagag
       61 atgacgatct ttgaaacggt tgtaacagat gttggtatag aagcagaagt attcaggaaa
      121 gaaaaaataa tgattttatt tggagatgaa gcaccggaaa cattaacaga ttattgttat
      181 aagattgaat tgaatcaggt aagaaaagaa ataacttgtg atatgaagct ctgttttgat
      241 aaacaaatgt atgtaatcac agcagtagga gatgttgtcc aaactaattt gaatgaatta
      301 ggacatatca cgataaaatt tgatggatct catgagccgg aacttccagg aacaatgtat
      361 gtagaagata aggaaatgcc gcatttaaga aaggggacaa tcattaaaat cgatcaagag
      421 gatcagaatg ggggaaagct atatggaaat catgcttgat acagcaaata caaaaagtat
      481 ttattcttgt caacagtttt tgaatttaac aggcgtaaca acaaatccta caattctgaa
      541 aaaagaagga gcgtcaaact tcttctcgca tatgaaagaa atcaaaaaaa taatcggaca
      601 gattcctatc catattcaga caataggaag aacggaagaa gaaatgatag aggatgctcg
      661 agttatcata gaagaactgg gaaaagatac gtttatcaag gtgccggtga atcaagcagg
      721 ccttcaagcg atcaaaaagt tgaaaaaaga aggttatttg attaccggta cagctattta
      781 cacagaatta caagggtatt tagctattaa taatggagca gattatattg caccttatta
      841 taacaggatg caaaatcaag gcattgatgc tatgaaagtg attgattcgt tgcggactga
      901 gatcgaacgt acgcaaagac caacaaaaat attagcagct agtttcaaaa attgtgaaca
      961 agtcactaca gccattcgat ctggagctca tgcgataacg ttaggtaaag agctactaga
     1021 tcaagtatta gagatgccct cgattgataa agcagtgagg ggcttccaag aagattgggt
     1081 gcaaactttc caagcagaaa cgattggtga actaaaaaag gtcgctgatt acttctgaat
     1141 caatttctcc ataaaataaa cgatagcctt ctcgaaaact cgaggaggct atctgacgat
     1201 tcaatattca ttaatttaaa gtttgggtga ctattactta tttctcattt aaacgatgta
     1261 ttccctaacc tctaatacat ccttgtaact ctgaagttac gccagttttg aataaatttc
     1321 ataaaaacaa atgaaaagtg tcgcataaaa atatctttcg tgtttctcta gtataacaga
     1381 ataagaggaa caagtccaga agggatattt ttagtctttt ttaaaataac ttttcagtcg
     1441 agtttgagtt tatgatagtt gtttttgaag cgatacggca agtgaaaaaa tagggaagca
     1501 tgaaagaatc tacttaatgg cgcattgatt catgtgatag aaataaaaaa cttttttaaa
     1561 ttatatcctc atgattgtaa caaatccagt gatttgtttt ggtacaaata aaaaaaggcc
     1621 ccgatgaagt tgtggcaaaa gtggtttgcc tcaaaaataa gaaggaattt ccaaaaatca
     1681 cttctcaaat tttgccacat ccccaattgg gtcaaatttg tttatttgaa tatatttttt
     1741 acataaggat ctttaatagt tacattgtct tttttcatca ctttacggat tgttttatcc
     1801 atgaattccg tatcaagagt ttttgcttcg gtagcaattt tttctagctc agatttgtat
     1861 ttgtctttat tagaaccttt atcctgtttt tttaccattt ttactagata atagctttgc
     1921 tgataagtcg ttggatctgt tacaggaatg atatcagaaa cttccccgtt ttttagttta
     1981 aatgctgctt tttttacttc agtaggaatc tctggattgg ttgaatcaaa attcattttt
     2041 ccatcagttt cctttaatgt tttatttttt ccataagctt gaacaagctt ggagaaattt
     2101 tccccttcat ttgcttcttt ttttgcttta tctgcttctt ctttagtcga aaatagaatg
     2161 atctgtgtac taacttctgg atagaattct ttccaagcag tgtctagatc tttatctgtt
     2221 aattttacat tggctttcaa accgtattgg aaagccagca tctgtttaaa gagtttctta
     2281 tatgtctctt ctgtttggcc aactgctttt aattgttcct tgtagtttgg accgagtttt
     2341 ttgatttgtt cattatattc ttttgttact tcctttgaag aaatctcttt tccgtataca
     2401 tcatcaaatg ctttgaagac aatcattttt tttaatagtg tttgattatt tgatttaact
     2461 tttgggttat cataaaaatc ctgaactgta agtgttccac ctttaaacgt tgcaatcgtt
     2521 tcattggact tgctacaacc tgagaatagt aaaattgcac atgctgaaag gcacataatt
     2581 agtagttgtt ttttcaaaaa atttcccatc ctttccttat taaaaacagt ctaacagaag
     2641 aattagggag acagtctttc attttcgtaa ggttgctgta gatattctta gttcgtaaaa
     2701 atctattctc tctatgatgg agaacattta catagttttc acgagacaag aaagaaaagg
     2761 gttgttaaac ttaatataaa ggagggatga aagatgatca ggattattcg gttgattgga
     2821 tctgttttaa tagtcttagt tggttttgtt ttaggaattc ttgtcaataa tgcacttgcg
     2881 gatatgttta ttggaaatag tgaatggatt ggagcaattg ccggtacggt cggacattta
     2941 gctgtttttt tactagcttt atttcttgct agtaaggtcg aaggaaaaag aatagaagat
     3001 tatggtgttt ctgttcgagg aaaagactgg gggtatttag cgggaggtct cgttgttgga
     3061 attggagtgt ttctgcttat tactagtcca ttatatttga cgggggcgta tcggttagat
     3121 tcaggaaacg caaacattgt gccgctgata acgagtttta ttttatttat tgcagtgggg
     3181 gcatcagaag agcttttatt ccgtggcttt tttcagcatc agatgcttag atttggtccg
     3241 ttaatagcaa tgattggatc tgctgcatta tttgctcttt tgcacggatt gaatcctaac
     3301 atgacatttc tggcagtttt caatatcttt ttagcaggtt gctttttcag cgcacttatc
     3361 tacagtacag gaagtttatt tacggctatt ggtgctcata ttacttggaa ttggacacaa
     3421 ggagctattt tgggtatacc agtcagcgga acgcaagaac aaggtttttt ctccactctg
     3481 atccaatcgc aaaatacaat aattactggg ggaaactttg gggctgaagc atctatatca
     3541 actacattat tcttgttagt cttgattgtt ggtttgttac tctatgcata taaaacaaag
     3601 cggatgaaga aatacactgc tgtttatcaa taaaagatga agaaagcaag gctgatatcg
     3661 gtcttgcttt tttaagtctg tcattgatta aacgaaaaag taaacaaatt caagcgaata
     3721 cacgacatct tttgttagat tttcacaact gactgtcgtg ttctcatcat tttcatttta
     3781 tataattcct ttatcaaata gaaggtaatt atatggagag tgatagaaat gaagaaaaca
     3841 gcgaccgtat tatttgttat tcttgggctg agtataattg cagcaatgat tggaatcagt
     3901 acttctgcaa atgaacgggc aaatgagaat ctttcattta ctgtgaaaac tgatcggatt
     3961 gtttatgaca tgacacaaca agtcattact attccagtca aaccaaacaa aagcgtgaat
     4021 gctagtgatg tacatgctgt tctaacatat ggttgggatg gtaatggaag ttcagaaaaa
     4081 gttattgggg aagtttattt aaaagacgta caatggaccg ctggtattga gtatacaata
     4141 atgatttctg cagaactatc gattgatgag atcaagagta aagataaagt tgatttgatt
     4201 gttttttatg atggacagat gacgatcacg gagaatttga aaccaagcag ttggacagtt
     4261 gttggaccgc caagcagtac ggatagcaca agtagtgaaa gcagtacaga aaacacgagc
     4321 ggtgaaagca gtacagagag tacgagcagt gaaagtagca cagaaaacac gagcagcgaa
     4381 agcagtacgg agagtacgag tagtgaaagt agcacaggaa gcacgagcag cgaaagcagt
     4441 acggagagta cgagtagtga aagtagcaca ggaagcatga gtagtgagag tagcacagga
     4501 agcacgagca gcgaaagcag cacaggaaaa gtaaataatg aacttgattt ggtacctatc
     4561 ataccaacta aggaacaaaa gagaaatgca gggagcaacc taaaaagtgt gctaaaacag
     4621 cctgattttg tttctcgtgg aattgatcat caagaaaaag cgaaagaaac agataataga
     4681 aaaagcgatt taccagaaac tggtagtaga accttgaaca agctaattac atggatgggt
     4741 gtgttactca ttttgattgt aggagcaagc tattttcgtt ctctcttcca tcgagtgaaa
     4801 taaagaataa aagcaacaga tctaaaattg aataaaacga tagatttttg agtgaatatt
     4861 tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt tgccagatct atttttgttt
     4921 actaacatat ttgagaaaca ggaaataaca ggaacacaaa tggaaaataa gagcaagaat
     4981 aattgacaat gcacactgcg catggtagta tctctgaaaa atcagtaacg aatctatttg
     5041 tacgttattc atgtagctgt cggaataagt ctaaataata gataaaaagg agtatgtact
     5101 ttgtttgaag aatttattga tatcaatgaa aggcaagtat atcaattttt gaactattgt
     5161 tatgaaagag acgagaaatt atatgtggtg aaggatatcg ctttagatct aaactacacc
     5221 ctggctaaga tgaattcggt gattcaacaa gcagaatcgt tttgcgaaag gtatccagaa
     5281 tataaattat cttttctttc agaaaacaag atgataaaag tcgagttttc ttctcagttt
     5341 ttactgagta aagtttattc tatactttta gagggaacga ttggttatat tttactggat
     5401 tcactttaca aaggtactta tcaatcctta gaaaatctta gtcaaaaaat tatataagtt
     5461 tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat tttgaaaaac tatcatttat
     5521 cttgcagctt gaaaaaaggt aatccgatag aaggagaaga ataccgtatc cgatactttt
     5581 ttcacttgat gtattggcag tcattcgacg aaaatcattc tgatatatta tcaagtataa
     5641 aaaataaacg tcaactatta gaaaaatttg cgaaacatgc gccatatacg cgtagtatag
     5701 atagaaagaa atggatcagc ctactaagaa tttcgttgca acgaatgaaa caaggccact
     5761 cgattacgta tgtcccagaa gagatgaaca aatttgtgca tccattgata acaaaagaag
     5821 agtttacgca ttacatacta gagccttttt ttgaagcaaa cttctggtcg atgaatcaca
     5881 tcactccaac agagcaagct tacctttatt ttatgtttgg tgtaatgaac acctatttgg
     5941 aagaagattt gttggatact ccagataaag tacaagaaag ttggggctgg attgaatcgt
     6001 ctattgtaga aaattttgaa aaaaacttca agatttgttt tacaacttca gaaaaaaatt
     6061 atctatatgt caatttggca atgatccatt tgtattcaag ggtttttggg acaaaaaaga
     6121 aggtggatgc ttttggaaaa tcagcagaag acagagattt tcagcatata tttccagtga
     6181 tgtatccaat catgcaacag ttttttagtg gggtagctcg cagaattcca gagcttaata
     6241 cttattatga aaagaacaga cggttgattt ttcaatactg tatgttagtc agggatattt
     6301 ttataaaaca cgaacaacct gtcatattct atattcaatc aaagtatggt aaaacacagg
     6361 aattatgggc aaagaaaagg atattatcta tgactgaacg cccaataaaa tatagtgcat
     6421 ccgctgatca gttacctgat attgtcatta gcgattatcc tatcgacaaa aaggcatatg
     6481 atcaaacgaa tacacagata ttttactgga atggacaacc tacaaaaaat gattgggcgc
     6541 gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa tagatccata ttggaaaaat
     6601 tggatttcgt taattaagaa aagcagattg tttatgccaa aattaagaca ttgcaaaaag
     6661 attattttct ttaaaatcga caagatgacc agaaagcaag agttcgttga gctcttgctt
     6721 tcttttagtt ttagaaagga acatcctgat tttatcttcg ctaaatccag tcaaaagttt
     6781 tgcagattgg tcgtagataa tcggtttttt caataaagaa ggggatgtac gaatctcaat
     6841 atatagttca ctcaaactga gttcatctat tctgtgaaca agatttttat aactaataga
     6901 atgagtagag agcatttcgg tcacgccgtt gtttgtcaat tttagtaagt ggataaattc
     6961 atttttggtt agtggttgac gaattaagtt ctgttcgata aacggtatat catggacgat
     7021 aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa cctgtataca atttaatcat
     7081 catacattcc tccaatagct aagtatttat gaaataataa catgaagaaa tagtcgaaaa
     7141 gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt ataatggaac tagctagata
     7201 agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac
     7261 gcaatagtag tgatcgattt acaaaaaggg gtatgttttg attcttcgcc gatacacgaa
     7321 ttagaaacag tgacagcatt agtgaataaa agaatcaaaa catacgaaac gattgggaaa
     7381 ccagttattt tcgttcaaca cgaagatgct caattagtgc cacaaacaaa accatgggag
     7441 ctattgtcgg aattatctat tcctgagcac agttattttg tgagaaaaac ccatgcgaat
     7501 tcattttata agactaattt gagtgtttta ttaactgaat tggactgtca ttcgatcgaa
     7561 ttatgtgggg cacaagcaga gtattgtata gatacgactg tgaagtttgc tcatggatta
     7621 ggataccagc ttatgatgtg tagaggagca acgacaactt atgataatcc ttttatgaaa
     7681 gcaaaggaaa caatcgcttt ttacgagaaa atttgggata aacgatttct gacatttttg
     7741 gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg atactggttt atccattctg
     7801 ctttattacg taaaatgaga aaataatcct gttctatttt tatgggataa accatttatt
     7861 ttttgtataa aaaatgtttg cgtttacata taattctcct agaaacataa aaaggagtga
     7921 atagcttgag aacaaaacaa gtattaaagg gagcgttggt catcttattt gctgtaagta
     7981 caagcgtccc gcaatggaca gtatatggag agagcatttc ttccgaatcg gaatctcggg
     8041 aggtagaaac cacagaaact actgatgctg gaaatgaaac gaaatcgagt acttcacaaa
     8101 cagaaacaaa tactgaaaca acggttgaga catctgttac tagcacagat caagaaacga
     8161 ccgctaccac agataaccaa caaacagaag aagcaacagc tacaaaaatg tcaaacgacg
     8221 aatcctcttc tgctaaagta agtgacaaag agaatgggac agaaaatgat gatatcggta
     8281 aaggtgaaaa tttcaatggt aaagaatatt tgaattactt cggtaaacag atgacagaaa
     8341 tacttaatgg accaacagaa gacatcaagt caaaattaat agagttatta gtcactgagc
     8401 ttgctgttaa tgattatggt gtagataaag agattatcaa acatctgatt gctacttatc
     8461 tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag
     8521 atgatgggac aacgcctaca gaaaacacag gcttctatcc aaatacaaaa caacttcatc
     8581 aaactgtact aaacaaacaa gatggcaaaa tgatggatgt atatgcatat tatgtcgatc
     8641 aaggttcaga taaaacagtc atgatccatg gcggatttcg agggaattgg aataacggga
     8701 tcgttacaga agaatacaat gatttttata aggccgggta taatttatta tttgttgatt
     8761 ccagagcgac tggtaatagc ggaggcgatt atgtgaccta tggacaatat gagtcagatg
     8821 acgtccttta ctggataaat caagaagttc gagagcggcc atctcaaaag attttacttt
     8881 atggtggttc gatgggagca gctacgatga tgtctgtatt agcgaaagat attcctgtca
     8941 atgtcaaagg aattattgaa aattgcggat ttgcatcaat tgatgaacaa ctacgtttta
     9001 cgtacagcca gacagtagcc cccgcactac cggatgccat aaaaaatcag ttagacatca
     9061 ttggggatca agagcatgaa gatttattta tgggcctttt gaaacaatac tattttgatc
     9121 aggagatgca tttggataca aaagctgctc tacctacgat tggaatgtca gattcattgc
     9181 ctaaattgat tatccatgga acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat
     9241 tatatgagtt atcaggaggg tataaagatc ttttattagt agaaggagca gggcatggaa
     9301 aggcacagga agttgaccat gctgcctaca cgaaacatgt aacggacttt ttgaaagtag
     9361 tgttccatga tcagataaac gtaaagtatg tggatgaaaa caataaaagt ctactcaaag
     9421 accaggacga aatccagctt tacggagctt atggagaaaa ttatatgaca gagcaaaaga
     9481 cattcgaagg atacgaatta gcaaatgttg aaggaccgac tgaagggatt tttaatgaga
     9541 cgataccaac tattattttc tattacaaaa aaatcccagt agctccacca aaaaagcaag
     9601 atccgacaga gaatgcagac aataagggaa agaatgaacc tcgagaggat caatcaagtg
     9661 atgatacgca accgattcag atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa
     9721 gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca ggtggatttt
     9781 tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa atgaatgaaa
     9841 tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt agtcactgta
     9901 tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa acaatttgac
     9961 acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa aatcgaacaa
    10021 atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa tatggatatc
    10081 tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca tggaggcgca
    10141 tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg ttcagacacg
    10201 cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca tcctacacca
    10261 gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga tcaagcagat
    10321 tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg agaatatttg
    10381 ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac tttggagaac
    10441 aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc acttactcaa
    10501 gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta ctttgataat
    10561 aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt
    10621 tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa acaaatggaa
    10681 gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag
    10741 aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca agcgcaagaa
    10801 acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca aaagaggttg
    10861 taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc ttttcatggt
    10921 ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac cttttattcg
    10981 aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt ttataagtca
    11041 gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag
    11101 ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc cgttgttatt
    11161 gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag cgaaaaaaag
    11221 caaagaaata tggatagctt tcataagcag cacctccttt tttataattg tatgataaat
    11281 agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg atcatctagc
    11341 gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc agataggtac
    11401 aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata atagaaaaga
    11461 tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac ttttttattt
    11521 tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa aatgaggcca
    11581 aaagccagtg ttatcaagga aatacttaat tcaccaaaat tttttttaat tttttagaaa
    11641 ataagaaaca aaaataataa aaaaaagaaa agataaattt tttactactg tatactaact
    11701 ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg
    11761 atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa
    11821 aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct
    11881 gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg
    11941 tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg
    12001 agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa
    12061 aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt
    12121 tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg
    12181 agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg
    12241 gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt
    12301 tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat
    12361 agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga
    12421 aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt
    12481 ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc
    12541 tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc
    12601 cagttcactt gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat
    12661 ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag
    12721 tcttatggag acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt
    12781 tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt
    12841 aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat
    12901 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
    12961 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
    13021 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
    13081 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
    13141 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
    13201 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
    13261 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
    13321 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
    13381 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
    13441 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
    13501 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
    13561 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
    13621 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
    13681 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
    13741 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
    13801 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
    13861 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
    13921 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
    13981 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
    14041 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
    14101 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
    14161 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
    14221 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
    14281 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
    14341 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
    14401 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
    14461 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
    14521 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
    14581 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
    14641 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
    14701 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
    14761 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
    14821 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
    14881 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
    14941 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
    15001 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
    15061 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
    15121 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
    15181 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
    15241 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
    15301 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
    15361 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
    15421 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
    15481 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
    15541 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
    15601 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
    15661 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
    15721 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
    15781 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
    15841 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
    15901 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
    15961 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
    16021 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
    16081 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
    16141 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
    16201 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
    16261 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
    16321 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
    16381 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
    16441 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
    16501 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
    16561 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
    16621 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
    16681 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
    16741 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
    16801 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
    16861 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
    16921 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
    16981 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
    17041 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
    17101 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
    17161 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
    17221 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
    17281 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
    17341 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
    17401 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
    17461 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
    17521 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
    17581 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
    17641 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
    17701 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
    17761 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
    17821 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
    17881 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
    17941 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
    18001 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
    18061 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
    18121 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
    18181 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
    18241 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
    18301 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
    18361 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
    18421 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
    18481 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
    18541 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
    18601 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
    18661 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
    18721 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
    18781 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
    18841 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
    18901 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
    18961 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
    19021 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
    19081 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
    19141 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
    19201 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
    19261 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
    19321 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
    19381 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
    19441 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
    19501 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
    19561 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
    19621 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
    19681 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
    19741 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
    19801 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
    19861 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
    19921 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
    19981 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
    20041 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
    20101 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
    20161 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
    20221 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
    20281 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
    20341 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
    20401 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
    20461 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
    20521 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
    20581 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
    20641 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
    20701 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
    20761 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
    20821 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
    20881 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
    20941 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
    21001 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
    21061 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
    21121 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
    21181 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
    21241 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
    21301 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
    21361 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
    21421 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
    21481 aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa
    21541 tcgtccatat gagggtttcc a
//
LOCUS       DACERL010000049        20801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.052, whole genome shotgun sequence.
ACCESSION   DACERL010000049 DACERL010000000
VERSION     DACERL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.052"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..300
                     /locus_tag="IXF54_001859"
     CDS             <1..300
                     /locus_tag="IXF54_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1405114.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IXF54_001860"
     CDS             complement(724..894)
                     /locus_tag="IXF54_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1405115.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IXF54_001861"
     CDS             complement(1088..1267)
                     /locus_tag="IXF54_001861"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405116.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1453)
                     /locus_tag="IXF54_001862"
     CDS             complement(1277..1453)
                     /locus_tag="IXF54_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405117.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="IXF54_001863"
     CDS             1654..2766
                     /locus_tag="IXF54_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1405118.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IXF54_001864"
     CDS             complement(2844..3137)
                     /locus_tag="IXF54_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1405119.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IXF54_001865"
     CDS             3305..3619
                     /locus_tag="IXF54_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1405120.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IXF54_001866"
     CDS             3640..5619
                     /locus_tag="IXF54_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1405121.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IXF54_001867"
     CDS             5632..5958
                     /locus_tag="IXF54_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1405122.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IXF54_001868"
     CDS             5974..6492
                     /locus_tag="IXF54_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405123.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IXF54_001869"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IXF54_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1405124.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IXF54_001870"
     CDS             complement(7928..9433)
                     /locus_tag="IXF54_001870"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1405125.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IXF54_001871"
     CDS             complement(9525..10115)
                     /locus_tag="IXF54_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1405126.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IXF54_001872"
     CDS             10294..11940
                     /locus_tag="IXF54_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1405127.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IXF54_001873"
     CDS             11989..12708
                     /locus_tag="IXF54_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1405128.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IXF54_001874"
     CDS             13187..14134
                     /locus_tag="IXF54_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1405129.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IXF54_001875"
     CDS             14146..15066
                     /locus_tag="IXF54_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1405130.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IXF54_001876"
     CDS             15107..16564
                     /locus_tag="IXF54_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1405131.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IXF54_001877"
     CDS             16909..17523
                     /locus_tag="IXF54_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1405132.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IXF54_001878"
     CDS             17516..19240
                     /locus_tag="IXF54_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1405133.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IXF54_001879"
     CDS             19237..20688
                     /locus_tag="IXF54_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405134.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6708 a         3510 c         4167 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acagttctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACERL010000050        20664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.053, whole genome shotgun sequence.
ACCESSION   DACERL010000050 DACERL010000000
VERSION     DACERL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.053"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            168..362
                     /locus_tag="IXF54_001880"
     CDS             168..362
                     /locus_tag="IXF54_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405135.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IXF54_001881"
     CDS             631..930
                     /locus_tag="IXF54_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405136.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IXF54_001882"
     CDS             complement(1027..1461)
                     /locus_tag="IXF54_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405137.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IXF54_001883"
     CDS             complement(1693..2403)
                     /locus_tag="IXF54_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1405138.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IXF54_001884"
     CDS             complement(2393..3253)
                     /locus_tag="IXF54_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1405139.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IXF54_001885"
     CDS             complement(3258..3896)
                     /locus_tag="IXF54_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1405140.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IXF54_001886"
     CDS             complement(3911..4210)
                     /locus_tag="IXF54_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405141.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IXF54_001887"
     CDS             complement(4253..5737)
                     /locus_tag="IXF54_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1405142.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IXF54_001888"
     CDS             complement(5757..6218)
                     /locus_tag="IXF54_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1405143.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXF54_001889"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXF54_001889"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1405144.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IXF54_001890"
     CDS             7521..8291
                     /locus_tag="IXF54_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1405145.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IXF54_001891"
     CDS             complement(8564..9391)
                     /locus_tag="IXF54_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405146.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IXF54_001892"
     CDS             9959..11194
                     /locus_tag="IXF54_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1405147.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IXF54_001893"
     CDS             complement(12193..12453)
                     /locus_tag="IXF54_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1405148.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IXF54_001894"
     CDS             complement(12480..13781)
                     /locus_tag="IXF54_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405149.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IXF54_001895"
     CDS             complement(14073..14531)
                     /locus_tag="IXF54_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1405150.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IXF54_001896"
     CDS             complement(14544..15869)
                     /locus_tag="IXF54_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1405151.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IXF54_001897"
     CDS             complement(15886..17298)
                     /locus_tag="IXF54_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1405152.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IXF54_001898"
     CDS             complement(17315..18079)
                     /locus_tag="IXF54_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1405153.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IXF54_001899"
     CDS             complement(18183..19058)
                     /locus_tag="IXF54_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1405154.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IXF54_001900"
     tRNA            complement(19313..19397)
                     /locus_tag="IXF54_001900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IXF54_001901"
     tRNA            complement(19415..19486)
                     /locus_tag="IXF54_001901"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IXF54_001902"
     tRNA            complement(19496..19568)
                     /locus_tag="IXF54_001902"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IXF54_001903"
     tRNA            complement(19630..19714)
                     /locus_tag="IXF54_001903"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IXF54_001904"
     tRNA            complement(19735..19805)
                     /locus_tag="IXF54_001904"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IXF54_001905"
     tRNA            complement(19851..19922)
                     /locus_tag="IXF54_001905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IXF54_001906"
     tRNA            complement(19934..20006)
                     /locus_tag="IXF54_001906"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IXF54_001907"
     tRNA            complement(20009..20082)
                     /locus_tag="IXF54_001907"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IXF54_001908"
     tRNA            complement(20089..20169)
                     /locus_tag="IXF54_001908"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IXF54_001909"
     tRNA            complement(20171..20246)
                     /locus_tag="IXF54_001909"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IXF54_001910"
     tRNA            complement(20282..20354)
                     /locus_tag="IXF54_001910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IXF54_001911"
     tRNA            complement(20364..20436)
                     /locus_tag="IXF54_001911"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IXF54_001912"
     tRNA            complement(20452..20523)
                     /locus_tag="IXF54_001912"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IXF54_001913"
     tRNA            complement(20538..20627)
                     /locus_tag="IXF54_001913"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6531 a         4319 c         3275 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACERL010000051        20323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.055, whole genome shotgun sequence.
ACCESSION   DACERL010000051 DACERL010000000
VERSION     DACERL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.055"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            292..1431
                     /locus_tag="IXF54_001914"
     CDS             292..1431
                     /locus_tag="IXF54_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1405155.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IXF54_001915"
     CDS             complement(1498..2073)
                     /locus_tag="IXF54_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1405156.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IXF54_001916"
     CDS             complement(2078..3052)
                     /locus_tag="IXF54_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1405157.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IXF54_001917"
     CDS             3335..4303
                     /locus_tag="IXF54_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1405158.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IXF54_001918"
     CDS             4416..4694
                     /locus_tag="IXF54_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1405159.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IXF54_001919"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IXF54_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1405160.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IXF54_001920"
     CDS             5160..5906
                     /locus_tag="IXF54_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1405161.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IXF54_001921"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IXF54_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1405162.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IXF54_001922"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IXF54_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1405163.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IXF54_001923"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IXF54_001923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1405164.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IXF54_001924"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IXF54_001924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1405165.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IXF54_001925"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IXF54_001925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1405166.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IXF54_001926"
     CDS             10643..11185
                     /locus_tag="IXF54_001926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1405167.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IXF54_001927"
     CDS             11208..12764
                     /locus_tag="IXF54_001927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1405168.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IXF54_001928"
     CDS             12778..13680
                     /locus_tag="IXF54_001928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1405169.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IXF54_001929"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IXF54_001929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1405170.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IXF54_001930"
     CDS             15123..15545
                     /locus_tag="IXF54_001930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1405171.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IXF54_001931"
     CDS             16014..16244
                     /locus_tag="IXF54_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1405172.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IXF54_001932"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IXF54_001932"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1405173.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IXF54_001933"
     CDS             17655..17831
                     /locus_tag="IXF54_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1405174.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IXF54_001934"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IXF54_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1405175.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IXF54_001935"
     CDS             18326..18709
                     /locus_tag="IXF54_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405176.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IXF54_001936"
     CDS             18878..19762
                     /locus_tag="IXF54_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1405177.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IXF54_001937"
     CDS             19879..20112
                     /locus_tag="IXF54_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1405178.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IXF54_001938"
     tRNA            20200..20280
                     /locus_tag="IXF54_001938"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
BASE COUNT         6752 a         3553 c         4418 g         5600 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gac
//
LOCUS       DACERL010000052        19578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.056, whole genome shotgun sequence.
ACCESSION   DACERL010000052 DACERL010000000
VERSION     DACERL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19578
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.056"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..504
                     /locus_tag="IXF54_001939"
     CDS             <1..504
                     /locus_tag="IXF54_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1405179.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNE
                     VIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVR
                     YMFNKIT"
     gene            602..790
                     /locus_tag="IXF54_001940"
     CDS             602..790
                     /locus_tag="IXF54_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1405180.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(848..1201)
                     /locus_tag="IXF54_001941"
     CDS             complement(848..1201)
                     /locus_tag="IXF54_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405181.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1732..2154
                     /locus_tag="IXF54_001942"
     CDS             1732..2154
                     /locus_tag="IXF54_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1405182.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2151..2381
                     /locus_tag="IXF54_001943"
     CDS             2151..2381
                     /locus_tag="IXF54_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405183.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2879..3079
                     /locus_tag="IXF54_001944"
     CDS             2879..3079
                     /locus_tag="IXF54_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1405184.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3106..4299
                     /locus_tag="IXF54_001945"
     CDS             3106..4299
                     /locus_tag="IXF54_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405185.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="IXF54_001946"
     CDS             complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="IXF54_001946"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1405186.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5991..6914)
                     /locus_tag="IXF54_001947"
     CDS             complement(5991..6914)
                     /locus_tag="IXF54_001947"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1405187.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7110..7715
                     /locus_tag="IXF54_001948"
     CDS             7110..7715
                     /locus_tag="IXF54_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1405188.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8023..9039
                     /locus_tag="IXF54_001949"
     CDS             8023..9039
                     /locus_tag="IXF54_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1405189.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9180..10481
                     /locus_tag="IXF54_001950"
     CDS             9180..10481
                     /locus_tag="IXF54_001950"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1405190.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10527..11189
                     /gene="deoC"
                     /locus_tag="IXF54_001951"
     CDS             10527..11189
                     /gene="deoC"
                     /locus_tag="IXF54_001951"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1405191.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11210..11608
                     /locus_tag="IXF54_001952"
     CDS             11210..11608
                     /locus_tag="IXF54_001952"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1405192.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11674..12756
                     /locus_tag="IXF54_001953"
     CDS             11674..12756
                     /locus_tag="IXF54_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1405193.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12968..14530
                     /locus_tag="IXF54_001954"
     CDS             12968..14530
                     /locus_tag="IXF54_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405194.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14523..15656
                     /locus_tag="IXF54_001955"
     CDS             14523..15656
                     /locus_tag="IXF54_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405195.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15656..16612
                     /locus_tag="IXF54_001956"
     CDS             15656..16612
                     /locus_tag="IXF54_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405196.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16711..17877
                     /gene="deoB"
                     /locus_tag="IXF54_001957"
     CDS             16711..17877
                     /gene="deoB"
                     /locus_tag="IXF54_001957"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1405197.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17901..18719
                     /locus_tag="IXF54_001958"
     CDS             17901..18719
                     /locus_tag="IXF54_001958"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1405198.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18736..19443
                     /gene="deoD"
                     /locus_tag="IXF54_001959"
     CDS             18736..19443
                     /gene="deoD"
                     /locus_tag="IXF54_001959"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1405199.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6258 a         3523 c         4216 g         5581 t
ORIGIN      
        1 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
       61 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
      121 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
      181 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
      241 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
      301 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
      361 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
      421 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
      481 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
      541 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
      601 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
      661 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
      721 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
      781 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
      841 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
      901 aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc
      961 ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata
     1021 caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat
     1081 aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc
     1141 gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca
     1201 tactaaatca cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa
     1261 catgttatat agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc
     1321 caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc
     1381 atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa
     1441 gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta
     1501 ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca
     1561 tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta
     1621 ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga
     1681 gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca
     1741 tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa
     1801 gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt
     1861 tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat
     1921 ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa
     1981 gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat
     2041 atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat
     2101 cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag
     2161 cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa
     2221 ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga
     2281 cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa
     2341 cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag
     2401 cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat
     2461 tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa
     2521 ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa
     2581 aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg
     2641 actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca
     2701 caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct
     2761 agagctgttc gattgctaag atattcttct cttgtcagtg tattattata aaactatttg
     2821 atataattat aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat
     2881 gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga
     2941 catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg
     3001 aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa
     3061 tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca
     3121 ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa
     3181 gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa
     3241 tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg
     3301 tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga
     3361 acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga taaaatggct
     3421 gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt
     3481 aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt
     3541 gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag
     3601 tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa
     3661 gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg
     3721 cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta
     3781 accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa
     3841 acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa
     3901 tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt
     3961 cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc
     4021 catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga
     4081 gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga
     4141 acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc
     4201 atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta
     4261 gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga
     4321 agagccagaa acccttggta taagaggatt tctagctctc ctacaactat tcccactcca
     4381 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     4441 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     4501 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     4561 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     4621 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     4681 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     4741 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     4801 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     4861 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     4921 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     4981 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     5041 atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc
     5101 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     5161 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     5221 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     5281 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     5341 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     5401 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     5461 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     5521 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     5581 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     5641 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     5701 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     5761 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     5821 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     5881 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     5941 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     6001 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     6061 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     6121 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     6181 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
     6241 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
     6301 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
     6361 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
     6421 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
     6481 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
     6541 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
     6601 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
     6661 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
     6721 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
     6781 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
     6841 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
     6901 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
     6961 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
     7021 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
     7081 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
     7141 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
     7201 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
     7261 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
     7321 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
     7381 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
     7441 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
     7501 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
     7561 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
     7621 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
     7681 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
     7741 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
     7801 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
     7861 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
     7921 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
     7981 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
     8041 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
     8101 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
     8161 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
     8221 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
     8281 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
     8341 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
     8401 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
     8461 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
     8521 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
     8581 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
     8641 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
     8701 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
     8761 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
     8821 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
     8881 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
     8941 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
     9001 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
     9061 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
     9121 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
     9181 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
     9241 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
     9301 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
     9361 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
     9421 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
     9481 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
     9541 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
     9601 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
     9661 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
     9721 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
     9781 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
     9841 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
     9901 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
     9961 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    10021 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    10081 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    10141 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    10201 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    10261 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    10321 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    10381 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    10441 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    10501 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    10561 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    10621 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    10681 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    10741 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    10801 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    10861 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    10921 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    10981 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    11041 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    11101 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    11161 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    11221 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    11281 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    11341 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    11401 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    11461 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    11521 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    11581 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    11641 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    11701 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    11761 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    11821 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    11881 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    11941 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    12001 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    12061 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    12121 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    12181 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    12241 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    12301 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    12361 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    12421 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    12481 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    12541 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    12601 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    12661 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    12721 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    12781 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    12841 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    12901 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    12961 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    13021 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    13081 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    13141 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    13201 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    13261 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    13321 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    13381 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    13441 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    13501 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    13561 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    13621 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    13681 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    13741 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    13801 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    13861 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    13921 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    13981 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    14041 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    14101 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    14161 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    14221 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    14281 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    14341 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    14401 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    14461 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    14521 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    14581 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    14641 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    14701 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    14761 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    14821 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    14881 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    14941 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    15001 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    15061 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    15121 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    15181 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    15241 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    15301 gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta
    15361 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    15421 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    15481 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    15541 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    15601 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    15661 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    15721 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    15781 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    15841 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    15901 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    15961 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    16021 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    16081 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    16141 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    16201 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    16261 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    16321 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    16381 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    16441 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    16501 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    16561 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    16621 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    16681 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    16741 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    16801 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    16861 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    16921 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    16981 ggattgaata tcaaaactcc atttcgtgtg tttccgaacg gattccctga tgatctgatc
    17041 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    17101 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    17161 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    17221 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    17281 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    17341 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    17401 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    17461 gtccgcacga aaagcaatat ggatggcgtt gaccagctac tgaaagttat gaaaaacgat
    17521 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    17581 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    17641 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    17701 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    17761 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    17821 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    17881 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    17941 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    18001 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    18061 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    18121 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    18181 tgacctatcc agtacgtgtg atggccgggc taggagccca ttcagtcatt gtgactaatg
    18241 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    18301 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    18361 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    18421 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    18481 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    18541 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    18601 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    18661 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    18721 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    18781 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    18841 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    18901 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    18961 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    19021 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    19081 atcgaaaaaa atttccaagc ctttcatttt cctccaatta gtgatttcaa tctattactg
    19141 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    19201 gaagattctt tctacaaaga tgatttgaca gagacattcc aattagctga acttggcgtt
    19261 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    19321 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    19381 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    19441 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    19501 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    19561 ttaccaagag gttgtgaa
//
LOCUS       DACERL010000053        19450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.057, whole genome shotgun sequence.
ACCESSION   DACERL010000053 DACERL010000000
VERSION     DACERL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.057"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            131..3658
                     /locus_tag="IXF54_001960"
     CDS             131..3658
                     /locus_tag="IXF54_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1405200.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IXF54_001961"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IXF54_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1405201.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IXF54_001962"
     CDS             7446..7787
                     /locus_tag="IXF54_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405202.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IXF54_001963"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IXF54_001963"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1405203.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IXF54_001964"
     CDS             9144..11564
                     /locus_tag="IXF54_001964"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1405204.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IXF54_001965"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IXF54_001965"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1405205.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="IXF54_001966"
     CDS             12998..13735
                     /locus_tag="IXF54_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405206.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="IXF54_001967"
     CDS             13749..14564
                     /locus_tag="IXF54_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1405207.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="IXF54_001968"
     CDS             14577..15224
                     /locus_tag="IXF54_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1405208.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="IXF54_001969"
     CDS             15240..15896
                     /locus_tag="IXF54_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1405209.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="IXF54_001970"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="IXF54_001970"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1405210.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="IXF54_001971"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="IXF54_001971"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1405211.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="IXF54_001972"
     CDS             18238..18756
                     /locus_tag="IXF54_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1405212.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="IXF54_001973"
     CDS             18863..19363
                     /locus_tag="IXF54_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1405213.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca
//
LOCUS       DACERL010000054        19342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.058, whole genome shotgun sequence.
ACCESSION   DACERL010000054 DACERL010000000
VERSION     DACERL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.058"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..456)
                     /locus_tag="IXF54_001974"
     CDS             complement(<1..456)
                     /locus_tag="IXF54_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1405214.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IXF54_001975"
     CDS             complement(561..1424)
                     /locus_tag="IXF54_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1405215.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IXF54_001976"
     CDS             complement(1620..3719)
                     /locus_tag="IXF54_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1405216.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IXF54_001977"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IXF54_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1405217.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IXF54_001978"
     CDS             complement(5072..6913)
                     /locus_tag="IXF54_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1405218.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IXF54_001979"
     CDS             complement(7095..9488)
                     /locus_tag="IXF54_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1405219.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IXF54_001980"
     CDS             complement(9595..10455)
                     /locus_tag="IXF54_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1405220.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IXF54_001981"
     CDS             complement(10514..11035)
                     /locus_tag="IXF54_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1405221.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IXF54_001982"
     CDS             11355..12074
                     /locus_tag="IXF54_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1405222.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IXF54_001983"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IXF54_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1405223.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IXF54_001984"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IXF54_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1405224.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IXF54_001985"
     CDS             16434..16790
                     /locus_tag="IXF54_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1405225.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="IXF54_001986"
     CDS             complement(17031..18035)
                     /locus_tag="IXF54_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1405226.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="IXF54_001987"
     CDS             18201..19052
                     /locus_tag="IXF54_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1405227.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="IXF54_001988"
     CDS             19069..19314
                     /locus_tag="IXF54_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405228.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5396 a         3992 c         3383 g         6571 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaagaa
    19321 tggaacggat tatgtatata ta
//
LOCUS       DACERL010000055        18956 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.059, whole genome shotgun sequence.
ACCESSION   DACERL010000055 DACERL010000000
VERSION     DACERL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18956
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.059"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..563)
                     /locus_tag="IXF54_001989"
     CDS             complement(<1..563)
                     /locus_tag="IXF54_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1405229.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IXF54_001990"
     CDS             complement(750..1400)
                     /locus_tag="IXF54_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405230.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IXF54_001991"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IXF54_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1405231.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IXF54_001992"
     CDS             complement(2912..3910)
                     /locus_tag="IXF54_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1405232.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IXF54_001993"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IXF54_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1405233.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IXF54_001994"
     CDS             complement(4926..5657)
                     /locus_tag="IXF54_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1405234.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IXF54_001995"
     CDS             complement(5661..6926)
                     /locus_tag="IXF54_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1405235.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IXF54_001996"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IXF54_001996"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1405236.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IXF54_001997"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IXF54_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1405237.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IXF54_001998"
     CDS             12268..13770
                     /locus_tag="IXF54_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1405238.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IXF54_001999"
     CDS             13772..14509
                     /locus_tag="IXF54_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405239.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            14515..14673
                     /locus_tag="IXF54_002000"
                     /pseudo
     CDS             14515..14673
                     /locus_tag="IXF54_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(14680..15873)
                     /locus_tag="IXF54_002001"
     CDS             complement(14680..15873)
                     /locus_tag="IXF54_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405240.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="IXF54_002002"
     CDS             complement(15952..16158)
                     /locus_tag="IXF54_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ1405241.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="IXF54_002003"
     CDS             complement(16151..17293)
                     /locus_tag="IXF54_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1405242.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="IXF54_002004"
     CDS             17805..18440
                     /locus_tag="IXF54_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405243.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="IXF54_002005"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="IXF54_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         5484 a         3984 c         3312 g         6176 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtat
//
LOCUS       DACERL010000056        18817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.060, whole genome shotgun sequence.
ACCESSION   DACERL010000056 DACERL010000000
VERSION     DACERL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18817
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.060"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..207
                     /locus_tag="IXF54_002006"
     CDS             <1..207
                     /locus_tag="IXF54_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ1405244.1"
                     /translation="TNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVIPENWRK
                     SLQNDQLLKTISQRFCDTVLSQRI"
     gene            276..542
                     /locus_tag="IXF54_002007"
     CDS             276..542
                     /locus_tag="IXF54_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1405245.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            532..801
                     /locus_tag="IXF54_002008"
     CDS             532..801
                     /locus_tag="IXF54_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1405246.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            1489..2256
                     /locus_tag="IXF54_002009"
     CDS             1489..2256
                     /locus_tag="IXF54_002009"
                     /inference="COORDINATES: protein motif:HMM:NF025424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405247.1"
                     /translation="MKNGTIKRMADLKSALREENQKKQLSIFDETLGYAAIDFRIDHQ
                     QKGERSISWVTLIAAPSVKMKTVGEFLERLEVYPDQTVVATVGLEGGYSPVHLAMDGL
                     GKETWLVLCDEIAYHNFFSPPEHSELEVEFDLKGAVKEHLKLQLETSKNPTETRLAGW
                     LEQQEDSELLIGVLKEEKSLSELVQFCIQKASASKGKQSAMMVSDEVVFQWVREYYTT
                     DIEMKNSSSVKGTMQTTPTPQVIETERKEEEQQISLF"
     gene            2262..3587
                     /locus_tag="IXF54_002010"
     CDS             2262..3587
                     /locus_tag="IXF54_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405248.1"
                     /translation="MKIAYDYPFKPLTPPKGFFKWCSSQITGYRWANKERTIVSTTRP
                     EAPIISKRLTKASNLNRSERCYPFALVLSSKTRVEIQSFGIWVGIENGVETYEIARTN
                     LECFCNDVHYQQHYFRNKWYGGLLNHGYMTGSYTNTQFYPNKWREKLAKNSPLKYCNL
                     KDISWYHLAHAYKYRREIEFLKLIHADKFADDMLFSAMHVIRDRVRGKSIDYRTVNMK
                     WLRKHKGILKNSTITFCEMRLKEAIRKCHGKPIAGIEEILTVEDLKRIPTCVGLTSFQ
                     KWAVAQDLDFGMYVDYLNMLDELGIGVDKENAMPKEFRAAHDEKAKLVGVKENKEYEK
                     EFNERVLQLKGLEKRLGDVQIVAPKEMGEMLVEGQNLRHCVGGQYYIRRHAKGETTIL
                     FVRKADKLDEPYLTLEMRNHRIVQLRGKRNQAAPPEIEQIIEKWVKTAN"
     gene            4239..4451
                     /locus_tag="IXF54_002011"
     CDS             4239..4451
                     /locus_tag="IXF54_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405249.1"
                     /translation="MDALRKLEVTLYNKQWFTLIGIEACLKNQDGFSAEEVLDQIQGC
                     LEEYKHVEGAMMEEYQQQNFAKLETV"
     gene            4549..4776
                     /locus_tag="IXF54_002012"
     CDS             4549..4776
                     /locus_tag="IXF54_002012"
                     /inference="COORDINATES: protein motif:HMM:NF037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405250.1"
                     /translation="MRKTISLTSNNIKVSGHIGTWYVYASRVYHGRRLFLVEHETYGD
                     HAANLILDKTGNCVMEDVWNGWEDYEVYIES"
     gene            4930..5382
                     /locus_tag="IXF54_002013"
     CDS             4930..5382
                     /locus_tag="IXF54_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405251.1"
                     /translation="MICRYCNKKVLKLEGGQYHCANCELDFFENQLIEFRSGLYREPT
                     VETDPFFFQYTTTKELLQMTYVELLSRLKLARGVYRELRKKVYGHNSQADDLDRENLK
                     AVTSAKLVIENIMIEKHGYYPASVFESVIDKEVLKNKKQMREVEKKHG"
     gene            5375..5626
                     /locus_tag="IXF54_002014"
     CDS             5375..5626
                     /locus_tag="IXF54_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405252.1"
                     /translation="MVKFEDQPKQGYTGIRTLETIASVFEDIQASGDMGQGEKNLRYL
                     QLMNELEEDHGAMIVNPTKTDLNLPAVALYRKVSQARVL"
     gene            5651..5959
                     /locus_tag="IXF54_002015"
     CDS             5651..5959
                     /locus_tag="IXF54_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405253.1"
                     /translation="MDKSKEIIFMPDYLVKDHTVLVLDSANNLHGFLSIESTDDHFVF
                     TNDIDLLKTVPKIIAEELATDIRASEGDYIPVTNKDTGEVSELGSDDLQIVFSAVEWC
                     "
     gene            6033..6497
                     /locus_tag="IXF54_002016"
     CDS             6033..6497
                     /locus_tag="IXF54_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405254.1"
                     /translation="MNEPDMTNQFRVGEVILQRMIDRKEFEDIYRSVTRKNTVDAYYF
                     DAGRRIVTVFAYYLGSISGSSVDEERIIGELMALMATMERDSIPGVVVWDKISKLPGT
                     PEHSMQLASEKAIEDTFTFLLAAAKAEEPYYLNGGTETIVSMIAAEVEQDCR"
     gene            6516..6944
                     /locus_tag="IXF54_002017"
     CDS             6516..6944
                     /locus_tag="IXF54_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405255.1"
                     /translation="MLKCYGEAELLEMLKRNEFEEDSPIRSVEDLLGHLDQASPMMWV
                     TVYVPEVQSVYGIHRETLISTGQSHRSTSKPVALLEATGYQTSEHVFVSELAALLQME
                     VVPKNREIFELQLFERKRGKYMLRRWQMIGDQPVLIAQRG"
     gene            complement(7114..7980)
                     /locus_tag="IXF54_002018"
     CDS             complement(7114..7980)
                     /locus_tag="IXF54_002018"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HAQ1405256.1"
                     /translation="MITLKNHKLELLQFTDIHIGQAPFNEEDQKTFAMIDQTLAKTTA
                     DLIVITGDLIWSDGVIEPTKGLEALAEIFNKYPIPLAITYGNHDSEETIDRHDLHELE
                     KKLFNHLALKANQFFDPNQKECFTIEIKDDNQLVNVLYFIDSGANALIDYESYDWVSL
                     EQIKWYDETFAKYQKINHTKDLLFLHIPLPEYLQAGERIVEGRFWEMNPRISAPKLNT
                     GLFSHLLENNHLLGTFCGHDHDNNFEGIYLGQRLIYGNVTGYNCYGDLPRGYRKITLK
                     PDQMTTSIETFE"
     gene            8161..9108
                     /locus_tag="IXF54_002019"
     CDS             8161..9108
                     /locus_tag="IXF54_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1405257.1"
                     /translation="MKQDSYSVKDANVILSKLILGSSDYLKLDNKDKVDRMFTRYVEE
                     GGRTFDTARHYRESEAVIGSWLKDKKREDYTIITKGCHPVREAPDTPRVNPESILADL
                     TTSLEMLATDYVDVLLLHRDDPSQPVGPLMETLSQLVDEKKIRSFGVSNWELPRIIEA
                     KKYCEDNGLNPLSFNSPNFSLAKVNEPRWENCVTADDLMIAWHEETDFPLISWSAQAE
                     AFFGNRFSPDDVNDPEMAEFVAVYFNDLNWKRLVICEELAKEKGVKPIQISLAYVLSQ
                     TFPSYATIGPEEEWQLLDSVEAMNIQLSAEEIMRLKKGE"
     gene            9141..9878
                     /locus_tag="IXF54_002020"
     CDS             9141..9878
                     /locus_tag="IXF54_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1405258.1"
                     /translation="MYSVRKELEKDPENTFKSLKEIGFEAVQLDGMRGHKETEIKALV
                     DKYGFKIAGMHIKHDRFFNDRDGIISESLLFGCKTIYDKYIDDEEQHTKGYIQTKQQL
                     VTTQATLGKLGFRIGLHCPEYDFNQTVEGRTILDYITDPVFGTAVYAEPDTYWISVAG
                     KDPLEEFRKFSGRAPIVHLKDYIKGFAPEDMDNNLTELGNGDVDFKAIVEWGEKSGVE
                     YYCIEQDYSKIGIFNSLAKSFDHLMSI"
     gene            9895..11148
                     /locus_tag="IXF54_002021"
     CDS             9895..11148
                     /locus_tag="IXF54_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1405259.1"
                     /translation="MKKNSRIVMMGVALVSGLVLAGCGNGSASNSDKVELSINYYNGA
                     ISKKTMDNAKKEFSDYKLSFKQIPANDDFDTKLKASLNSKSAPDITAINSNISDYLPY
                     ADKFVNLLDYDSETLAADFVEWKWDSCFTNDKKTQIAMPIDIGPTALFYNVDAFAKAG
                     LPTNDQEISKALKDEESLMAAAKQMKEKADMPFFQSSLSLLQSKNRTMTEHIYDENGK
                     LTYANGQLKEIWDYAVKAEKSGYTLGTKGNSADGANSVQKGMFGGMIEASWGINDLAE
                     NGVSPKQWMIAKSPFTASNYGGSYLAVIKTSSHPKEAAEVVKYLTNEDSQRANYEELA
                     LFPSNTKVYDDSFKAAKNELFGDEEFNHYFIDAANELEYVPSDPRESAAFKCFEDQII
                     LVAEQGKDPEKAWNDAVKKVEEMSK"
     gene            11249..12154
                     /locus_tag="IXF54_002022"
     CDS             11249..12154
                     /locus_tag="IXF54_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1405260.1"
                     /translation="MNSESKSKKKFSFDKVAPYVFVSPFYILFLIFGLFPVIFSGVLA
                     FSKWDGLGDIQFVGLDNFTRLLRDADFYLSVKNTLIIWAVGTFPMLFLALILAFLVNL
                     KFIKHKELLKSIFFLPNITSVVAVTILFGLIFSNLDGLANALLQFLGFEPIKWVTSPF
                     WVRIVIALINIWMYLGYNMIIYLTGITKIPNDYYEAAVIDGATNMQIFWNITIPQLKP
                     IILFTVMMSTIGGLQVFSEAQVLVPQGATPEGGAMTIVYYLYNTAFTNNEYGYGSAVA
                     WALVLIILVFSLINWYLTTYRIKGE"
     gene            12157..12981
                     /locus_tag="IXF54_002023"
     CDS             12157..12981
                     /locus_tag="IXF54_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1405261.1"
                     /translation="MSNRIFKILVRAFLAIGVFLSIFPFYWMIVMASKDNSEIYHIPP
                     DLMLGSELMNNIKTVFTETTFFSSLLNTLFVALTSTLLILFFDSLAGFSFAKLNFKGK
                     AFLFTFLLGTMMIPGQLNIIPSFYLMDKFGWVGSYKALIIPGMANAFGIFWIKQYCTD
                     AIPDSLLESAKLDGCTLFKAYYKVVLPIMKPALAFLALYSFMGAWNDYMWPLIILNDE
                     SKYTLMLALTQLQGLYKTNYPLVITGTLLATLPLLLVFTIFSKQLISGITEGAVKQ"
     gene            complement(13094..13381)
                     /locus_tag="IXF54_002024"
     CDS             complement(13094..13381)
                     /locus_tag="IXF54_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1405262.1"
                     /translation="MKEKNPYYRLKYVRIYKRRGKKRAIITIARMMLTAIYHMLSTGE
                     VWNPTDLYKIDMPDNLVQKQKDKAIKQATKLLISEGLLPDDFVRKDLEPAI"
     gene            13717..14307
                     /locus_tag="IXF54_002025"
     CDS             13717..14307
                     /locus_tag="IXF54_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405263.1"
                     /translation="MEVNMKEKFIALQAKLMDLILLSGLILIGAMTIIGFFYGIVAAF
                     VYLETKSEDKWQVLKKRQTILISLLLEMSAVFVAGIFFLNARLLSQLQGGATIIISFF
                     LAVCTLILIPLYFILLHKLAVGTIFTKETFLNSVKVILIGFPTWFTQIILMIALSVLT
                     YLFPALSIITVGYLITRTNKIINVQKMKLASLQEVY"
     gene            14307..16316
                     /locus_tag="IXF54_002026"
     CDS             14307..16316
                     /locus_tag="IXF54_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1405264.1"
                     /translation="MKLSQREIDLLKLALDGEEETTVPQLKKQLKLSVHQIRYAIDKV
                     NRFLIEKKLNQIFIEKDRLVIEQRRKIKKEIDFFISHSTPEQFKFTAEQIEYFISLKL
                     LLSDQRLPINYFVETLSTSRTSVVNVLDRITQELAEEEIELSNVPRKGYMIEEYSFKR
                     FSYFITILKKMINIREIYSFYYQDNLYSKAGDLVFFDLFDLDILFDSLGETIQYSKQM
                     ASEIDDTNFLFLTILNYKYRELINAFEMTDRTEKLQMISSFISEIKQNNPNQMENNNL
                     AKRLIRYINRELADVFEVKDFKVASNALLYHIQRFIFRQENGIPFRDIDSEVLVKDHK
                     NLYYVIFAAIRSFQNGLFESVEAPEAVLITLYYINEIEKNAPKVFKAPRILIICGEGR
                     AISSVLKIKISNLIDSKWIDTISVFEFETPLIDHYDLIITTVRLPEVESDKVLYSDNV
                     FSESFFEQIKLKLSVGGYSFATQRLGKLSQIMNAIVEEVNDDINMNRLEAKIIKILAN
                     NLLQNNRPNEQKFIFDASMLKYHQAAQTWQNAIRISGQELLAKHSIEEQYVNNIISNI
                     ENFGPYMIIAPGVLLAHAGEQDGVLNNGFSMHVFHEPITFPGENVFPVSVIMTIAVKD
                     RTSYHVVERIVQWVLDEETIFSLADEKNRQRIEQMIRNILMMQEV"
     gene            16324..16629
                     /locus_tag="IXF54_002027"
     CDS             16324..16629
                     /locus_tag="IXF54_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1405265.1"
                     /translation="MTTDEISFALISKAGTAFSMMIEALGDAREKDFEKAEEKKQEAE
                     ALMNEAHNLQTNLIVNEVNGDDNQINALLIHAQDTLMNTILMSTIVDEFLNLYKAGE"
     gene            16631..16966
                     /locus_tag="IXF54_002028"
     CDS             16631..16966
                     /locus_tag="IXF54_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405266.1"
                     /translation="MEKLSICLACNLGSSTSILVNKMKQVAAGSQKLSSFDLMIDAIP
                     AGNISTEQVKDYQIIMLGPQIGHRRNEIEQKVASINIPVTVIDAADYGSMNAANILKE
                     AIYQIKTAN"
     gene            16979..18214
                     /locus_tag="IXF54_002029"
     CDS             16979..18214
                     /locus_tag="IXF54_002029"
                     /inference="COORDINATES: protein motif:HMM:NF014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405267.1"
                     /translation="MTLDYINQVVGKIQTKMIPTLGKIEGNQVFKSMKKTFLYLNYVL
                     LISSVLAILKLVNEKFIHQQQIADLTSKLLLLLMDNFGWFFLGILCFLMFQEKGKFHY
                     YLCSAALYLMFSAKDLNLLSASKIETLFLALLALLVSFVLVSGYQLVLRGIKKIIKTP
                     MEFLDNLLLMIYFSVIFMVIYQILSQLKGIHLENILSWLNIDNPMMVFVIVFLEMFLW
                     YMGINGYGILAPIVLLFAISNLNANFQAIATGEAPQFIFTPNFWDYFLSVTGSGITGA
                     IVILSLLSKKTTLKNLGRTAASGTLFSVSEPIVFGIPVVMNPYFFIPFVIGTPILGVI
                     QWYVFKLGWVSLPTYFVADLPLPFSSFLATMDWRSLILTAATIALATLMYWPFYKAFE
                     KSYVEKNNDDKYQDLDLDF"
     gene            18230..>18817
                     /locus_tag="IXF54_002030"
     CDS             18230..>18817
                     /locus_tag="IXF54_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1405268.1"
                     /translation="MLKSFFKIFCGNFILGLAYAKLMVPNEIINGGVTSLSLIFSNLT
                     SLNITLFTNVITVTLLAVSLLFLGKFFFFSSVVSSVSYLLFFNLFYRLPFSLHTNVVV
                     DFLLAVLLIAIGYYCCLSENSSTAGLDVFAVILNKKIPKISVAVFLRYINIAVLVLGL
                     FSYGIKAVILGVLFSFSFTKVMDLLMEKGADPRKFF"
BASE COUNT         5850 a         3233 c         4107 g         5627 t
ORIGIN      
        1 acgaatagtt atcaagcagc catattgact gcggtcaatt taggagggga tactgatact
       61 actgctgccg taactggtgc tcttgcgggt attctttacg ggggtgaagt gatccctgaa
      121 aattggagaa aatcgcttca aaatgaccag cttcttaaaa cgatcagtca gagattttgc
      181 gataccgtgc tatctcaaag aatttgaata atggatatga tgcaaatctt gtttgtacta
      241 acgttgtatt gacaaaaagg attttattta gtacaatgtt agtacaaatg atgaacggag
      301 gtagaatcat gtctcaatca accattacat ttaggattcc cgacgatgaa aaagaactgg
      361 tattagaata tgcaaaagca cacaattctt cattgaccga actttatcga aatgctgtac
      421 tggataaaat cgaagatgaa atcgatctac ggactttacg gagtgcaatt gaagtttcca
      481 aagagaaaaa agagagtggt atttctcaag aggaaatgga gaaactgcta aatgaattat
      541 aaagtcatct acatgccgca agcccagaag caattaaaaa aaatggacag aaaccaagct
      601 aaaataattg tcgcctggat cagaaagaat ttagaaaaca cgactgaccc gcgtcagcac
      661 ggaaaaggtt tgactggaaa taagagtggg gaatggcgct atcgaattgg aaactatcga
      721 atattggcta atatttgtga ttctgaaatt gaaatccaga tattctcaat tggacataga
      781 agtacaatat ataaaagata accgtttttg gaacgaagta gagtttcgaa agtcaatgta
      841 taatcttttg aattttgcat aataccacag gtagaaacta cccgatacta tcggttggtt
      901 tctgcttttt tatttggtct tgaagaagaa aacgagtgat ctgtggcaaa gaaagaaaca
      961 aaaaaaggac aaaactgttg cttcgcctgc ggtgcagtcg cccttggatc aaagcgcgtc
     1021 tgttttcttt tggctagtgg caaggtctaa ctgcttggtg gctacgtgcc ttcgccagtc
     1081 gcagctgcct tggttggctg cgtgcctccg ccaagtcggc cgacctcacc atttttgcct
     1141 acgcaaaaag gccaaaagga tttaaaggaa tccgtcgaag catagctcca acttctccgg
     1201 atcaaaaagc ggtaggaacc cgtgccgggt tcctctattt gaattttttg gttttgcgct
     1261 acatttttac cattgcagac gccgactagc tacgaaatag aatgagcctc caggctgcgc
     1321 ctactcattc tatttctcgc aagtggcttc gccaggcggc aagggcaagc aatgtgctac
     1381 gcacccttgc cgcctggctg ctgcatggta aaagcttcgc aacaaaacgc aaaaaattca
     1441 gcgtttggct actgctgtgt agcgcaaaga ccaaaaggag cgaaaaccat gaaaaacggt
     1501 actatcaaac gaatggcaga cttgaagtct gcactgaggg aagaaaacca gaaaaaacag
     1561 ctctcgattt ttgatgaaac gctcggatat gcggcaattg attttcgtat cgaccaccaa
     1621 caaaaaggtg aacgaagtat ttcgtgggtc acgttgattg cggcaccttc ggtcaaaatg
     1681 aagactgtgg gagagttttt ggaacgatta gaggtctatc ctgaccaaac agtagttgcg
     1741 acagtcggac tggaaggtgg ctactcaccc gtccatttag caatggacgg tctagggaaa
     1801 gaaacatggc tcgttctctg tgatgaaatt gcctaccaca attttttctc accacctgaa
     1861 cactcggaac tggaagttga attcgatttg aaaggggcag tgaaagagca cctaaaatta
     1921 caactagaaa cgtctaaaaa cccaactgaa acaaggcttg cgggttggtt agagcaacaa
     1981 gaagattctg agttattaat tggtgtctta aaagaagaaa agtccctatc cgaattggtt
     2041 caattttgta ttcagaaagc ttcagcaagc aaaggaaagc agtccgctat gatggtaagt
     2101 gatgaggttg tttttcagtg ggtaagggaa tattacacga ctgatattga aatgaaaaat
     2161 tcaagttcag tgaagggaac catgcagacc acaccaacac ctcaagtgat tgaaacagaa
     2221 cgaaaggagg aagaacagca gattagtctg ttctaactac tatgaaaatc gcatacgatt
     2281 atccatttaa accactcacg ccaccgaaag ggtttttcaa gtggtgtagt tcgcaaatta
     2341 cgggctatcg ctgggcgaac aaagaacgga caattgtttc gacaacacga ccagaagccc
     2401 cgattatttc caagcgattg acaaaagctt caaatttgaa tagatcagaa cgctgttacc
     2461 cctttgcgct cgtgttaagt agcaaaactc gtgttgaaat tcaatctttc ggcatttggg
     2521 taggaattga gaatggtgtc gaaacctatg aaattgctcg aacgaacctt gaatgtttct
     2581 gcaatgatgt acattatcag caacattatt ttagaaataa gtggtatgga gggttgctga
     2641 atcatggtta catgactggc tcatacacga atacgcagtt ctatcccaac aagtggcgag
     2701 aaaaactggc aaaaaattca ccgctaaaat attgtaatct caaagatatt tcttggtacc
     2761 atctcgccca cgcctataaa tatcggcggg agattgagtt cttaaaactc attcatgcgg
     2821 ataaatttgc ggacgacatg ttattttcgg ctatgcacgt tatacgggat agggtacgag
     2881 ggaaaagtat cgactatcga acggttaaca tgaaatggct tcggaaacac aaaggaattt
     2941 tgaaaaactc tacaattacg ttctgtgaaa tgcgattaaa ggaagctatc agaaagtgtc
     3001 atggaaaacc aatagctgga atcgaagaaa ttctgacggt ggaagacttg aaacgtatac
     3061 caacatgcgt tgggctaacc agctttcaaa aatgggccgt tgcacaagac cttgactttg
     3121 gcatgtatgt ggactacctc aacatgttag acgaactcgg aatcggtgta gataaggaaa
     3181 acgccatgcc aaaggaattc agagcagctc atgatgaaaa agcaaaactg gtaggcgtta
     3241 aagaaaataa agaatatgaa aaagaattta atgagcgagt attgcaattg aaagggcttg
     3301 aaaaacgtct aggtgatgtt caaattgttg caccaaaaga aatgggcgag atgttggtgg
     3361 aagggcagaa ccttcgccat tgtgtcggtg gtcagtatta tattagaaga catgcgaagg
     3421 gagaaacaac gattctcttt gttcggaaag cggacaagtt agatgaaccc tatctcacct
     3481 tagaaatgag gaatcaccgt attgttcaat tgcgtgggaa gagaaaccaa gcggcacctc
     3541 ctgaaatcga acagataata gaaaaatggg ttaaaaccgc aaactgataa gacgtttacc
     3601 agagtgatga aaagcacagc attctggtaa gccgcttatc gggtaaaact ggcgcttcgc
     3661 ctgcggtgca gttgcccttg gctcaaaacg cgacagtctt cttttgccta gtgacagaat
     3721 ctaaccgctt gatggctacg tgccttcgcc agtcgcagtt gctttggttg gctgcgttcc
     3781 tccgccaagt cagccgattc agtcattttt gccagggcaa aaaagcaaaa ggattgaaag
     3841 gtcttgctcg aagcatagct ccgacttctc gcaagacggc ggggaaatca cccgcctgaa
     3901 aattccgagc gaattttgtt ttgttcgtca atcgccgctc ctattccggt ttcattttgt
     3961 gtgcaaaggt tgtttgctgt ttgtggttat agatcaaacc cggtggcagg taaagggtat
     4021 acaagcagca aaatccgccg ccctttacct gccaccgggc ccgatctaga gcataaccac
     4081 agcaaacgac ctaagcacac tggaaaaaat gaacccatga caaataggaa caaagggcac
     4141 gactggacaa accaaaattg gaatttcttt aaagattcca gctccgccct agctcctaaa
     4201 aaaatgggtt tcaaaccaac aaaaaaggag ttttttacat ggatgcttta agaaaattgg
     4261 aagtcacact ttacaacaaa caatggttta cactgattgg gattgaggct tgtttgaaaa
     4321 atcaggatgg tttttccgct gaagaggttt tggaccaaat acagggttgt ctggaagagt
     4381 acaaacatgt tgagggggca atgatggaag aatatcagca acaaaatttt gctaagttgg
     4441 aaaccgtgtg ataccagctg aacaatcgga aatgcaaggc atgtaattcg gatgacgacc
     4501 tggtccttgc caggttgttt tccggaatca ttgaaagggt gttgaattat gcgaaaaact
     4561 atttcattaa catcgaacaa tatcaaggta agcgggcata taggtacgtg gtatgtatac
     4621 gccagtcgag tgtatcacgg acgtcgttta tttcttgtag aacatgaaac ctacggggat
     4681 cacgctgcca acttgatact ggataaaacg ggaaactgtg tcatggaaga tgtttggaat
     4741 gggtgggaag attatgaggt gtatattgaa agctgaaggg aaagagtagg caccagtctg
     4801 atcttaggat gacctaagct gagagtcttc agcttgggta tgttcttgta ggctaattct
     4861 tggtaaaaaa gaagagcagc attgtttatt tagcatgata tactatttgt gaaaacgggg
     4921 ggatttgtga tgatttgtcg atattgtaat aaaaaggttt tgaagctaga agggggccag
     4981 tatcactgtg ccaattgtga gctggatttt tttgagaacc aactgattga gttcaggtct
     5041 ggattgtatc gagaaccgac cgtcgaaaca gatccgttct ttttccaata cacgacgaca
     5101 aaagaattat tgcagatgac ctatgtagaa ttgctgtctc ggctgaagct ggcgagaggc
     5161 gtctatcgtg agttgaggaa aaaagtttat ggccacaata gccaagcaga tgatctggat
     5221 cgcgagaacc tgaaggcagt cacttcagca aagcttgtca tcgaaaatat tatgattgaa
     5281 aaacatggct attatcccgc cagcgttttt gagtctgtta tagataaaga agttttgaaa
     5341 aataaaaaac aaatgcgaga ggtggaaaaa aagcatggtt aaatttgagg atcaaccgaa
     5401 gcaggggtat acaggtatcc ggacgctaga aacgattgcg agtgtatttg aggatattca
     5461 ggccagcggt gacatgggac agggcgagaa gaatttgcga tacttgcagc tgatgaatga
     5521 gttggaagag gaccacggag ctatgattgt gaatccgact aaaacggatc tgaatttgcc
     5581 tgcggtcgca ttataccgga aggtttctca agctcgagta ttataggcgg aacttgagag
     5641 gagtttacga atggataaat cgaaagaaat catctttatg ccagactatt tagtaaaaga
     5701 tcatacggtg ctagttttag attcagcaaa caaccttcac ggttttttga gtatagaatc
     5761 aaccgacgat cattttgttt ttacaaatga tatagacctg ctcaaaaccg tcccgaaaat
     5821 tattgcggaa gaacttgcaa ctgatattcg cgcttctgaa ggcgactata ttccagtaac
     5881 aaataaagat actggcgaag tatctgaatt gggttcagac gacctccaaa tcgtattttc
     5941 tgcggtagag tggtgctaaa ggtagcggct gagtctaacg atcatgatga atgcatggat
     6001 tcaactaata ggagaaaaga aggtagaaat taatgaacga gccggatatg acgaatcagt
     6061 ttcgagttgg ggaagtcatt cttcagcgaa tgattgatcg gaaagaattc gaagacattt
     6121 atcgctcagt gacccgaaag aacacggtgg acgcctacta tttcgatgca gggcgtagaa
     6181 tcgtaacagt atttgcttat tacttgggaa gtatctctgg ttcttcagtc gatgaggaaa
     6241 gaatcattgg ggagcttatg gctttgatgg caacaatgga gcgcgacagt attcctggtg
     6301 ttgttgtttg ggataaaatt tcaaaacttc caggcacacc ggaacattcc atgcagcttg
     6361 cttcagaaaa agcaatcgaa gacaccttta cttttttact tgcagctgca aaggctgaag
     6421 agccgtatta tctcaatggt gggacggaaa ctatcgtatc aatgattgct gctgaagtag
     6481 aacaggattg taggtagcag caggaggcag aaaatatgtt gaaatgctat ggtgaagctg
     6541 agctactaga aatgcttaag agaaacgagt ttgaagagga ttcaccgatt cggagtgttg
     6601 aagatctgct tggacacctg gatcaggcca gtccaatgat gtgggtgacg gtgtatgtac
     6661 cagaagttca aagtgtgtac ggtattcatc gagagacgtt gatttctacg ggacaaagcc
     6721 ataggagtac atctaagcca gttgctttgt tagaggcgac aggttatcaa acaagtgaac
     6781 atgtctttgt ttctgaatta gcggcgcttt tacagatgga ggtagtcccc aagaatcgag
     6841 aaatctttga attacagctg tttgagcgaa aacgggggaa gtacatgttg cgtcgctggc
     6901 aaatgattgg ggatcagcca gtactaattg cccaacgtgg ctaattatta tttaaagatg
     6961 agtgttgtgt aagctgaggt gacctttatt ggatgaaaag caacaaaaac gacaactact
     7021 aattaaatta gaatcagtcc taaaatgaat gactatttta ggactgattt tgctacataa
     7081 aaaacagcta gcagattact agctgttctt tctttattca aaagtttcga tggaagtcgt
     7141 catttgatcc ggttttaagg tgatcttacg gtagccccgt ggcaaatcgc cataacagtt
     7201 ataaccggtg acatttccgt aaatcaatcg ttggcccaga tagatgcctt cgaaattatt
     7261 atcatgatca tgaccgcaaa aagtgccgag taaatggttg ttttctagta aatggctgaa
     7321 taagccggta ttaagctttg gtgccgaaat tcgcggattc atttcccaaa aacgcccctc
     7381 tacgatccgt tcgccggctt gtagatattc aggtaatgga atgtggagga acaatagatc
     7441 ttttgtatga ttaatctttt gatatttcgc gaaagtttcg tcgtaccact tgatttgttc
     7501 taaagaaacc cagtcgtagc tctcataatc aattaaggca ttggcgccgg aatcgataaa
     7561 ataaagaaca ttaactaatt gattatcatc tttaatttca attgtaaaac attctttttg
     7621 atttggatca aagaattgat tggcttttaa agccagatga ttgaagagtt ttttctctaa
     7681 ttcgtggaga tcgtgacgat caatggtttc ttcggagtcg tgattgccgt aagtgattgc
     7741 caatggaatc gggtatttat tgaaaatttc ggctaaagct tctaaccctt ttgtaggttc
     7801 aataacacca tctgaccaaa ttaagtcgcc ggttatcacg atcaaatcag cggttgtctt
     7861 cgctaaggtt tgatcgatca tggcaaaagt tttttgatct tcttcattaa aaggagcttg
     7921 accgatgtgg atatcggtaa attgcaatag ttcaagtttg tgatttttta aagtaatcaa
     7981 gggtaacatc ctttcttaat acgttattaa aataaaccct atcaaaaaat aatgcaaacg
     8041 ctttaataac tttttttaag attaaaaaaa taaaatatgt cattcaagac gttgaaagcg
     8101 attttagaat ttgctattct ttgggtgtgt tgaagagagc aaaaagaaag gggcttcaga
     8161 atgaaacaag attcttattc agttaaggat gcaaacgtta ttttatcaaa attgatcttg
     8221 ggttccagtg attatttgaa gttggacaac aaggacaagg tcgataggat gttcactaga
     8281 tacgtagaag aaggtggacg aacttttgac actgccagac attaccgtga aagtgaggcg
     8341 gtgattggca gttggttaaa ggataaaaaa cgtgaggatt acactattat cactaaaggt
     8401 tgtcatccag tgcgggaagc accagatacg ccgcgtgtta atccagaaag tatcttagca
     8461 gacttgacta ctagtttaga aatgttggca actgattatg tcgatgtgct tttactgcac
     8521 cgagatgatc ctagccagcc ggtcggtcca ttaatggaaa ccttaagcca attggttgat
     8581 gaaaagaaaa tccgatcttt tggtgtttct aactgggagt taccacgaat tattgaggca
     8641 aaaaaatatt gtgaagataa cggattaaat cctttgtcat tcaatagtcc aaattttagt
     8701 ttagccaaag ttaatgagcc tcgttgggaa aactgtgtga cagcagacga tctgatgatt
     8761 gcttggcatg aagaaactga ttttcctttg atttcttggt cggcacaagc agaagcattc
     8821 tttggaaatc gcttctcacc ggatgatgtt aacgatccgg agatggcaga gtttgttgct
     8881 gtttatttta atgacttgaa ttggaagcgc ttagtgattt gtgaagagtt ggcaaaagaa
     8941 aaaggtgtca agccaatcca aatttctctg gcctacgtgt taagtcaaac tttcccctca
     9001 tatgccacaa ttggtccgga ggaagaatgg cagttacttg attcagttga agcgatgaat
     9061 attcaattat ctgctgaaga aatcatgcgt ctgaagaaag gagaataagc cagttggata
     9121 aaacaaagat tgcggcccaa atgtattccg tccgtaaaga actggaaaaa gatccagaaa
     9181 atacttttaa atcattgaag gaaattggtt ttgaagccgt tcagcttgat ggtatgcggg
     9241 ggcacaaaga aactgaaatc aaagcattag ttgataagta tggtttcaaa attgccggga
     9301 tgcacattaa acatgatcgt tttttcaatg atcgagacgg aattatttct gaatcattat
     9361 tgtttggttg taaaacaatc tacgataaat atatcgatga tgaagagcaa catacgaaag
     9421 gctatatcca aacgaaacaa caattggtca cgactcaagc gactcttgga aaactcggtt
     9481 ttagaatcgg ccttcactgt ccggaatatg attttaatca aacagtcgaa gggcggacca
     9541 ttttggatta tattacagac ccagtttttg ggacggcggt ctatgcggag ccagatactt
     9601 attggatctc ggtagctgga aaagatcccc tagaagaatt tagaaaattc tctggtcggg
     9661 caccgattgt tcatttgaaa gattacatca aaggttttgc accggaagat atggataata
     9721 acttgacgga attaggtaac ggagacgtcg attttaaagc cattgttgaa tggggcgaaa
     9781 aaagtggtgt tgagtactac tgtatcgaac aagattattc caaaatcggt atttttaaca
     9841 gtttagcgaa aagttttgat cacttaatgt caatttaatc ttaggagcga gaagatgaag
     9901 aaaaatagtc gtattgtcat gatgggtgtc gcattggtaa gtggtttggt attagcaggt
     9961 tgtggaaatg gcagtgcctc aaatagtgat aaagtggaat tatctatcaa ctattacaat
    10021 ggtgcgatca gtaaaaaaac catggataat gctaaaaaag aattttctga ctacaaattg
    10081 agttttaaac aaattcctgc caatgatgat tttgatacaa agctaaaagc cagcttaaat
    10141 tcaaaatcgg caccagatat cacagcaatc aacagtaata tttctgatta cctgccatat
    10201 gctgataaat tcgtaaacct attggattac gatagtgaaa cattagccgc tgactttgtt
    10261 gaatggaagt gggattcttg ttttacaaat gacaaaaaaa cgcagattgc catgccaatt
    10321 gatattggcc caacagcttt attctataat gtagacgctt tcgcaaaagc tggtttgcca
    10381 acgaacgatc aagaaatcag taaagcattg aaagatgagg aaagcttgat ggctgctgcc
    10441 aaacaaatga aagaaaaagc cgatatgcct ttcttccaat cttctctttc attgttgcaa
    10501 tctaaaaatc gcacgatgac tgaacatatt tacgatgaaa atggcaaatt gacctacgct
    10561 aacggtcaat taaaagaaat ctgggattac gctgttaaag cagaaaaaag cggttacact
    10621 ttgggaacaa aaggaaatag tgctgatggc gccaactccg ttcaaaaagg catgtttggt
    10681 ggcatgatcg aagcttcttg ggggatcaat gacttagctg aaaatggcgt ttcaccaaaa
    10741 caatggatga tcgcaaaatc tccatttacc gcgtctaact acggtggttc atatttggcg
    10801 gtgatcaaaa cttcaagcca tccaaaagaa gctgcggaag ttgtgaaata tttgacgaat
    10861 gaagacagcc aacgggctaa ctacgaagaa ttggctttat tcccatcaaa tactaaagtt
    10921 tatgatgatt ccttcaaagc agcgaaaaat gaattattcg gtgatgaaga atttaatcac
    10981 tactttatcg atgccgctaa cgaattggaa tatgtaccat cagatccacg tgaaagtgct
    11041 gctttcaaat gtttcgaaga ccaaatcatt ttggtagctg aacaaggcaa agatcctgaa
    11101 aaagcttgga acgatgccgt gaagaaagta gaagagatgt ctaaataatc agaatgtgat
    11161 ttcgggatat gacatcgatc aaattttaaa aattggtggg tgtcatagac ccgtaaatgg
    11221 aggtctaagg atggagattg gtgagatgat gaattcagaa tcgaaatcaa aaaagaaatt
    11281 tagttttgat aaagttgccc cttacgtttt tgtatccccg ttttatattt tgtttttgat
    11341 ctttggtctg tttccagtta ttttctcagg cgtcctggcc tttagtaaat gggatggctt
    11401 aggggatatc caatttgtcg gtttggataa ttttacccgt ttattgcggg atgctgactt
    11461 ttatttatca gtcaaaaata cgctaatcat ctgggcagtt gggacattcc cgatgttgtt
    11521 tttggcacta atattagctt tcttagtaaa tttgaaattt atcaaacata aagagttact
    11581 aaaatcgata tttttcttac caaatatcac ttctgttgta gcggtgacaa ttttatttgg
    11641 cttgattttc tcaaatctgg atggcttagc caatgcgcta ttacagtttt taggatttga
    11701 accaattaaa tgggttacgt caccattttg ggttcgaatc gtgattgctt tgatcaatat
    11761 ttggatgtac ttaggctata acatgatcat ttatctaaca gggattacaa aaattccaaa
    11821 tgattactat gaagctgccg ttattgatgg cgcaaccaat atgcagattt tttggaacat
    11881 tacgatacct caattaaaac cgattatctt gtttactgtg atgatgtcaa ccattggtgg
    11941 tttgcaagtc ttctctgaag cacaagtatt agtaccccag ggggcgactc ctgaaggtgg
    12001 ggcgatgacc atcgtttact acttgtataa tacggcgttt acaaacaatg agtacggata
    12061 tggatcagca gttgcttggg cattagtctt gatcattctg gtcttttcgt tgatcaactg
    12121 gtacttgacg acttatcgaa tcaaggggga ataaacatga gcaatcggat ttttaaaata
    12181 cttgttagag ccttcttagc gattggtgtc tttctttcga tcttcccatt ttattggatg
    12241 attgtaatgg ccagtaaaga taatagtgaa atctatcata ttccaccaga tttgatgcta
    12301 gggtctgaac tgatgaataa tatcaaaacg gtttttactg aaacaacatt ctttagctcg
    12361 ttgcttaata ctttatttgt ggcactaact tcgacactgc tgattttgtt ctttgattcc
    12421 ttagcgggtt tttcatttgc caagctgaac tttaaaggga aggcctttct ctttactttc
    12481 ttattgggaa ccatgatgat tccgggtcag ctgaatatta ttccttcatt ttatttgatg
    12541 gataaatttg gttgggttgg ttcttacaag gcgttgatta ttccagggat ggcaaatgcc
    12601 tttggtattt tctggatcaa gcagtattgt accgatgcca tccccgacag tttgttggaa
    12661 tcagcaaaat tagatggttg tacacttttt aaagcctatt acaaagttgt attgccaatt
    12721 atgaaaccag ctttagcatt tttagcactc tactctttta tgggtgcgtg gaatgactat
    12781 atgtggccat tgattatctt aaacgatgaa tcaaaataca cgttgatgtt ggctttgaca
    12841 caactacaag gactttataa aacaaattat ccattggtaa ttactggtac gttactggca
    12901 actttgccac tactcttagt ctttactatt ttcagtaagc aattgatttc tggtatcaca
    12961 gaaggtgccg ttaagcaata gaaagaggta ttagtgtgaa aacaaaacca cttagtatcg
    13021 gagatacacc caagaataga gcgcatggca aataaacgtc aaaaaccgca ggtctgacga
    13081 ttatgaaagt tatttagatg gctggctcta agtcttttct aacaaaatca tctggcaata
    13141 aaccttctga aatcagtaat ttggtcgctt gtttaattgc tttatctttc tgtttttgaa
    13201 caagattgtc tggcatatca atcttatata agtcagtcgg attccaaacc tcaccagttg
    13261 aaagcatgtg gtagatagct gtgagcatca ttcgagcaat cgtgatgata gcccgtttct
    13321 taccacgtcg tttgtaaatg cgcacgtact tcaaacggta gtacggattc ttttctttca
    13381 ccaaaacggc tgcgtgtgta catcaatacc acaacaaacg ggataaacga ctttcatgat
    13441 tcttcgcccc tttctaaaag tagcggagag gcagtgactg gtctgggcag catttaacag
    13501 gatttgttaa aacaatgatt agtgtgcgga cttctatagt tccgctcatt tgtgcttgaa
    13561 cgccccgacc ttacactggt taatatgctg gttcaaacaa tcgaggtttc ctacaactca
    13621 cctcaccgtg ctttgtagta tgcctctcct aattctatta taaacggaaa tccgcgtctt
    13681 cattactatt tgactcgagg agcggagcga gtcgagatgg aggttaatat gaaggaaaaa
    13741 tttatcgcct tacaagcaaa attaatggac ttgattttat tgagtggttt gatcttgatc
    13801 ggggcaatga cgataattgg tttcttttat ggaatcgttg cggcgtttgt ttatctagaa
    13861 actaaaagtg aagataaatg gcaagtttta aaaaaacgtc agacaatctt gattagtctc
    13921 ttattagaaa tgtcagcagt ttttgtagcc ggtatctttt tcctaaatgc gcgtttgctt
    13981 tcgcagttgc aaggcggtgc aacaattatc attagtttct ttttagccgt ttgtaccttg
    14041 atcttaattc cactgtattt cattttattg cacaagctag cagttggaac aatttttacg
    14101 aaggaaacat ttcttaatag tgtcaaggtc atcttaatcg gttttcctac ctggtttacc
    14161 caaattatct taatgatcgc gctgagtgtt ttgacttatc tgtttccggc tttatcgatc
    14221 attacagttg ggtacttgat tacccgcaca aataagatca ttaatgtgca aaaaatgaaa
    14281 ttagctagtt tgcaggaggt ctattaatga aattgagtca acgggaaatt gatcttttaa
    14341 agctggcttt agatggtgaa gaagaaacga ctgtgccgca actaaaaaaa caattgaagc
    14401 tctcagtcca tcaaattcgc tatgctattg ataaagtgaa ccgcttttta attgaaaaaa
    14461 agttgaatca aatttttatt gaaaaagatc ggctggtcat tgaacaacga cgaaaaatca
    14521 aaaaagaaat tgattttttt atatctcact cgacgccaga gcagtttaaa tttaccgcag
    14581 agcaaattga atattttatc tcattgaaac tgctgctaag tgatcaacgt ttgccaatca
    14641 attattttgt agaaacatta tcgacttccc ggacttctgt ggtaaatgtt ttggaccgaa
    14701 tcacacagga actggctgaa gaagaaatcg aattatcgaa tgtaccacga aaaggttaca
    14761 tgattgagga gtatagtttt aaacgatttt cctatttcat cacgattttg aaaaaaatga
    14821 tcaatattcg tgaaatctat agtttctact atcaagataa tttgtattca aaagctggcg
    14881 acttagtatt ttttgacctc tttgatttgg atatcctctt tgattcttta ggggagacga
    14941 ttcagtattc aaaacagatg gccagtgaaa ttgatgatac gaacttttta tttttaacga
    15001 ttttgaacta taagtatcgt gagttgatca atgcctttga gatgactgat cgaacggaaa
    15061 aattacaaat gatttcaagt tttattagtg aaatcaaaca aaataatccg aatcaaatgg
    15121 aaaacaacaa tctagccaag cgattgattc gctatatcaa ccgtgaatta gccgatgttt
    15181 ttgaagtaaa ggattttaaa gtggcctcta acgcactact atatcatatt caacgcttta
    15241 tttttagaca ggaaaatggc attccttttc gcgatattga tagcgaagtt ctagtaaaag
    15301 accacaagaa tttgtattac gtgatttttg cggcaatccg ttcttttcaa aatgggttgt
    15361 ttgaaagtgt agaagcacct gaggcggtat tgatcacatt gtattacatc aatgaaattg
    15421 aaaagaatgc accaaaagtt tttaaagcgc cgcgaattct gattatttgt ggtgaaggcc
    15481 gtgcaatttc cagcgtgtta aaaatcaaaa tcagtaattt gatcgattcg aagtggatcg
    15541 atacgatttc agtttttgaa tttgagacac ccttgatcga tcattatgat ttgatcatta
    15601 ctacggttcg gttgccggaa gtcgagtcag acaaagttct atactcggac aatgtcttct
    15661 ccgaaagttt ctttgaacaa attaaattga aactatcggt aggtgggtac tcatttgcga
    15721 cccaacgcct tggcaagctt tcacaaatca tgaatgcaat cgtcgaagag gttaacgatg
    15781 atatcaatat gaatcgtctt gaagcaaaga ttatcaaaat tttggcgaat aatttgctgc
    15841 aaaataatcg tcccaatgaa caaaaattta tctttgacgc cagcatgttg aaatatcatc
    15901 aagcagcgca aacatggcaa aatgcgattc gcattagcgg gcaggaactt ttggcaaagc
    15961 actcgattga agaacaatat gttaataata ttatcagcaa tattgaaaat tttggacctt
    16021 atatgatcat tgcaccgggt gtcttgctgg ctcatgctgg tgaacaagat ggcgtgctta
    16081 ataatggctt tagtatgcat gtttttcatg aaccgattac ttttccaggt gaaaatgttt
    16141 ttccggtaag tgtgatcatg acaattgccg taaaagatcg cacatcttat cacgttgtcg
    16201 aaaggatcgt gcaatgggtc ttagatgaag aaacgatttt ttcgttagct gatgagaaaa
    16261 acagacaacg gatcgaacaa atgatccgaa atattttgat gatgcaagag gtgtaacatg
    16321 acaatgacaa cggatgaaat atctttcgcc ttgatttcta aggctggtac agctttttca
    16381 atgatgatcg aggcattggg agatgcccgc gaaaaggatt ttgaaaaagc agaagaaaag
    16441 aaacaagaag ccgaggcttt gatgaatgaa gcccataatt tacaaacgaa tttgatcgta
    16501 aatgaagtca atggcgatga taatcaaatc aatgccttat tgattcacgc ccaagacaca
    16561 ttaatgaata caatcttgat gtcgacgatt gtcgatgaat ttcttaattt atataaggca
    16621 ggagaataaa atggagaaat tatcaatttg cttagcgtgt aatttagggt catccaccag
    16681 tatcttggtg aataaaatga aacaagtggc agccgggagt caaaaattgt cttcttttga
    16741 tttaatgatt gacgccattc cagcaggaaa tatcagcacg gaacaagtga aggattatca
    16801 aattattatg ttgggaccac aaatcggaca caggcgcaat gaaattgagc aaaaagtagc
    16861 tagtatcaat attccggtaa cagtcatcga tgcagctgac tatggcagta tgaatgccgc
    16921 taatattttg aaagaagcaa tttatcaaat caaaactgct aattaaggat ggatagccgt
    16981 gactttagat tatatcaatc aggtagttgg aaaaatccaa acaaaaatga ttccgacttt
    17041 aggtaaaatc gaaggaaatc aagtttttaa aagtatgaaa aaaacttttt tgtaccttaa
    17101 ttatgtctta ttgatttcct cggttttggc tattttgaaa cttgtaaatg aaaagtttat
    17161 ccaccaacaa cagattgctg atttgacgtc taagttgttg ttattactga tggataattt
    17221 tggctggttt tttctaggga ttctttgctt tttgatgttt caggaaaaag gaaagtttca
    17281 ctactatcta tgcagtgcgg ctttgtatct catgttttca gcaaaagatc tgaatttatt
    17341 atcagcctct aaaattgaaa cactattttt agcattgttg gctctgttag ttagctttgt
    17401 tctggtgagt ggttatcaat tagtgctgcg gggaattaaa aagatcatca agacaccaat
    17461 ggaattttta gataatttgt tactgatgat ttatttcagc gtgattttca tggttatcta
    17521 tcaaatattg tctcagctta aaggaattca tttagaaaat attttgagtt ggttaaacat
    17581 tgataaccca atgatggttt ttgtcattgt ttttcttgaa atgtttcttt ggtacatggg
    17641 catcaatggc tatggcatat tagcgccaat tgttttacta tttgccatta gtaatttgaa
    17701 tgcaaacttt caagcaatcg ccacgggcga ggcaccgcag tttattttca cgcctaattt
    17761 ttgggattat tttctaagtg ttactggatc aggaattact ggcgccattg tgattttatc
    17821 tttgttgagt aagaaaacaa cattaaagaa tctcggtcga acagccgcta gtggaacctt
    17881 gttttcagtt tccgaaccaa ttgtttttgg gataccggtg gtgatgaatc catacttttt
    17941 tatccccttt gtgattggga ctccgatttt aggggttatt caatggtatg ttttcaaact
    18001 tggttgggtt tcattaccga cttattttgt ggcagattta ccactaccat tttcgagctt
    18061 cttggcaacg atggattggc gttctttgat tttgaccgcc gcaaccattg ccttagcaac
    18121 tttgatgtac tggccgtttt ataaagcatt tgagaaaagt tatgtagaaa aaaataatga
    18181 tgataagtat caggatctag atttggattt ttagaaaaga ggaataaaat tgctaaaatc
    18241 tttttttaaa atattttgcg gaaactttat tttgggctta gcatacgcca aactgatggt
    18301 tccaaatgag atcattaatg gtggtgtgac gagtctttcg ttgatattta gtaatctgac
    18361 ttccctcaat atcaccttat ttacaaatgt catcaccgtg acattactgg cagtttcatt
    18421 gctcttttta gggaaatttt tctttttctc ttccgtcgtt agcagtgttt cttatttgct
    18481 gtttttcaat cttttctacc gcttgccatt ctcactacat acaaatgttg tggttgattt
    18541 tcttttggca gtattgctga ttgccatcgg ttattattgc tgcttgtcag aaaattcttc
    18601 tacagcaggt ttagatgtat ttgcggttat tttgaataaa aaaatcccca aaatctcagt
    18661 ggctgttttc ttgaggtata ttaatatcgc agttttagtt ttggggctgt tttcatatgg
    18721 aatcaaggcc gtcattttag gggttttatt tagttttagt tttactaaag taatggatct
    18781 tttgatggaa aaaggtgcgg atccccgcaa atttttt
//
LOCUS       DACERL010000057        18674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.061, whole genome shotgun sequence.
ACCESSION   DACERL010000057 DACERL010000000
VERSION     DACERL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18674
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.061"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            190..759
                     /locus_tag="IXF54_002031"
     CDS             190..759
                     /locus_tag="IXF54_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1405269.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            746..1606
                     /locus_tag="IXF54_002032"
     CDS             746..1606
                     /locus_tag="IXF54_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1405270.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1796..2500
                     /locus_tag="IXF54_002033"
     CDS             1796..2500
                     /locus_tag="IXF54_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405271.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2505..4340
                     /gene="walK"
                     /locus_tag="IXF54_002034"
     CDS             2505..4340
                     /gene="walK"
                     /locus_tag="IXF54_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1405272.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4337..5653
                     /locus_tag="IXF54_002035"
     CDS             4337..5653
                     /locus_tag="IXF54_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405273.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5654..6523
                     /locus_tag="IXF54_002036"
     CDS             5654..6523
                     /locus_tag="IXF54_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405274.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            6578..7387
                     /locus_tag="IXF54_002037"
     CDS             6578..7387
                     /locus_tag="IXF54_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1405275.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7490..8119)
                     /locus_tag="IXF54_002038"
     CDS             complement(7490..8119)
                     /locus_tag="IXF54_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ1405276.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8289..9581
                     /locus_tag="IXF54_002039"
     CDS             8289..9581
                     /locus_tag="IXF54_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405277.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9657..10319)
                     /locus_tag="IXF54_002040"
     CDS             complement(9657..10319)
                     /locus_tag="IXF54_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1405278.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10544..10828
                     /locus_tag="IXF54_002041"
     CDS             10544..10828
                     /locus_tag="IXF54_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1405279.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10879..12504
                     /gene="groL"
                     /locus_tag="IXF54_002042"
     CDS             10879..12504
                     /gene="groL"
                     /locus_tag="IXF54_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1405280.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12593..13741)
                     /locus_tag="IXF54_002043"
     CDS             complement(12593..13741)
                     /locus_tag="IXF54_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405281.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQIKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLTLHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(13836..14537)
                     /locus_tag="IXF54_002044"
     CDS             complement(13836..14537)
                     /locus_tag="IXF54_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405282.1"
                     /translation="MKKFFVTILKYLALIIGVLSCLMSFIVLPVTLVFLPLGIFLIFM
                     GLKPVEYLDGTIAKRKKEEKELAEKEQKEELNRMIRQLEKEDLSKRAALIDGVNLKSN
                     EFCYYTPKQRVKWQEQKTRTVRTNYSGLSSNIRIAKGLNYRMGSIKHNSNKVTEWNDV
                     FIGVPFLTNKRIIFVNENGMKVINLSNIVGIKAFSDGTHLYRESGKIILLRDFDATEF
                     NVILNRILNKDFEAH"
     gene            complement(14665..15174)
                     /locus_tag="IXF54_002045"
     CDS             complement(14665..15174)
                     /locus_tag="IXF54_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405283.1"
                     /translation="MTLDTKNKKFSKQLKYAQEYLEPGEKVVEGVFGTYETKSMGTDT
                     IKNGVFLATNKRLFFFGKRTFGFDSESFPYSNISSFEFAKKAMGYKISFYASGNKVNM
                     KWISIGDINSFVNFVKEKMEAKNHDHTEQNQSLTSPIQKVKELKELLDMDIITTDEFE
                     KKKKELLGL"
     gene            complement(15307..15738)
                     /locus_tag="IXF54_002046"
     CDS             complement(15307..15738)
                     /locus_tag="IXF54_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1405284.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGKT
                     AEAFHLSHELAHFTASHFEFSVLYDTSTTFHSKFETEADKVAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15756..16079)
                     /locus_tag="IXF54_002047"
     CDS             complement(15756..16079)
                     /locus_tag="IXF54_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405285.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLQEIADYFDVSVDYLLGREKKEAPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKA
                     LEENE"
     gene            16371..16562
                     /locus_tag="IXF54_002048"
     CDS             16371..16562
                     /locus_tag="IXF54_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405286.1"
                     /translation="MSNIDNGREAIKEFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            16577..17332
                     /locus_tag="IXF54_002049"
     CDS             16577..17332
                     /locus_tag="IXF54_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1405287.1"
                     /translation="MKELIKVTTNENNEQLVSGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFLQVEKAW
                     NSEEMILMRSRQILERKVETLQLENEEMKPKALFADSVSASHTSILVGELAKLIKQNG
                     VDIGSKRLFSWLREKEYLIKRKGTDWNMPTQKAMDLGLFEIKETTISHSDGHISINKT
                     PKVTGKGQVYFVNKFLGGVISEKTNTFRVDRSC"
     gene            17292..17591
                     /locus_tag="IXF54_002050"
     CDS             17292..17591
                     /locus_tag="IXF54_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405288.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFSEGIVRPGHSTTFIHYHTFIWFLKWKDANKYRVKILSPSDVLKEAS"
     gene            17639..17923
                     /locus_tag="IXF54_002051"
     CDS             17639..17923
                     /locus_tag="IXF54_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405289.1"
                     /translation="MKITVPDELIADELTEQIVRKVLDALDERLKVMNKSVELPPYPN
                     KSEVKKILGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            17944..18132
                     /locus_tag="IXF54_002052"
     CDS             17944..18132
                     /locus_tag="IXF54_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405290.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAVLRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            18457..>18674
                     /locus_tag="IXF54_002053"
     CDS             18457..>18674
                     /locus_tag="IXF54_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405291.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTNEELACDQ
                     DDLGNFVSIWDHVYLIHLSKQSRKPEYI"
BASE COUNT         6178 a         3136 c         3799 g         5561 t
ORIGIN      
        1 tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga
       61 aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa
      121 agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga
      181 gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca
      241 ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga
      301 acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc
      361 tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga
      421 tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact
      481 attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc
      541 tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa
      601 ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga
      661 gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc
      721 gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc
      781 aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa
      841 aattatttat aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta
      901 tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga
      961 aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag
     1021 tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga
     1081 agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc
     1141 agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt
     1201 agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg
     1261 acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc
     1321 atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt
     1381 ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc
     1441 tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga
     1501 gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac
     1561 tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa
     1621 acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa
     1681 gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag
     1741 cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa
     1801 aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc
     1861 caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc
     1921 tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga
     1981 agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga
     2041 ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc
     2101 attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc
     2161 tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca
     2221 tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt
     2281 cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt
     2341 acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg taactgtacg
     2401 tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg
     2461 tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc
     2521 gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct
     2581 cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat
     2641 ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga
     2701 atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa
     2761 acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag
     2821 gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca
     2881 atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca
     2941 atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa
     3001 gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct
     3061 cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac
     3121 agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga
     3181 aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag
     3241 ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg
     3301 tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga
     3361 tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga
     3421 ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa
     3481 acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag
     3541 tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc
     3601 atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt
     3661 cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag
     3721 gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag
     3781 accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata
     3841 cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt
     3901 ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa
     3961 gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga
     4021 ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc
     4081 ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga
     4141 accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga
     4201 caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg
     4261 cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg
     4321 aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat
     4381 ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc
     4441 aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc
     4501 cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa
     4561 cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct
     4621 ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg
     4681 ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt
     4741 agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca
     4801 attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga
     4861 agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa
     4921 atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc
     4981 gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa
     5041 tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt
     5101 ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg
     5161 agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag
     5221 caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga
     5281 ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg
     5341 tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa
     5401 tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat
     5461 catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg
     5521 ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta
     5581 tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt
     5641 ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg
     5701 gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat
     5761 tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac
     5821 ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct
     5881 tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt
     5941 gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt
     6001 gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat
     6061 tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact
     6121 agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat
     6181 cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca
     6241 tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat
     6301 caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct
     6361 agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt
     6421 agcgatcgaa acaagcgata aagtggtcca aattgagagc atcaacgctt tcagcaatac
     6481 gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga
     6541 aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt
     6601 taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa
     6661 aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca
     6721 aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca
     6781 tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca
     6841 gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt
     6901 tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca
     6961 tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt
     7021 gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta
     7081 tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt
     7141 aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat
     7201 gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa
     7261 caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg
     7321 cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc
     7381 gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg
     7441 gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga
     7501 ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg
     7561 cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac
     7621 ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat
     7681 aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag
     7741 ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg
     7801 tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa
     7861 gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt
     7921 tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat
     7981 ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca
     8041 cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc
     8101 caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg
     8161 ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt
     8221 cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga
     8281 gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga
     8341 atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg
     8401 ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag
     8461 acaaaacagg attttctatg tattttacac aagtatacca ggctacattt gatgtgatag
     8521 ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct
     8581 taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc
     8641 agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca
     8701 tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga
     8761 agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca
     8821 cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag
     8881 gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc
     8941 ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg
     9001 tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag
     9061 cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg
     9121 atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg
     9181 tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt
     9241 tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg
     9301 ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg
     9361 gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg
     9421 gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt
     9481 ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac
     9541 agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt
     9601 cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat
     9661 actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt
     9721 gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct
     9781 aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg
     9841 atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg
     9901 aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct
     9961 tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat
    10021 acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat
    10081 ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt
    10141 tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca
    10201 gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt
    10261 gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg
    10321 taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa
    10381 ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag
    10441 cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa
    10501 gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg
    10561 tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt
    10621 agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg
    10681 tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt
    10741 cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg
    10801 aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat
    10861 caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct
    10921 atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc
    10981 cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg
    11041 atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca
    11101 gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg
    11161 acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc
    11221 attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca
    11281 actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat
    11341 aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca
    11401 atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat
    11461 cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa
    11521 aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg
    11581 ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt
    11641 gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta
    11701 aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca
    11761 ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac
    11821 ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct
    11881 ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca
    11941 acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta
    12001 gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt
    12061 gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt
    12121 acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca
    12181 acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac
    12241 gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt
    12301 ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact
    12361 aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca
    12421 gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca
    12481 tcaatgatgg gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt
    12541 cttctttttt tgttttattt taagcaaaag gggcaaagaa gggacaaaaa tatcaaaggc
    12601 tatttagttt atcaattact tgttgtttgg ctctttttgt tacatggtta taaatagaca
    12661 gagttgtgtt tgcatcagaa tgccctacac gctccataat ggctttaaga ggtacgccta
    12721 attcggataa tagtgaaacg tggctatgtc tgaatatatg tgaggataga cttttttcta
    12781 attccaactc ttcctctact ttatggagta tagcgttaaa tgagtgcagc gtaagcggag
    12841 tgccacttgt agatataaat atatattgat cgggatctgt gggtctacct gcaagaatat
    12901 tgtcagctat cacactttca atcaattctt ttgcacgatt gggtaattgc acttcacgtt
    12961 gcgaataagt attttttgga gttgttttta tagcattatc cattttcaca gatgtgtaat
    13021 ctaaggtccc attaatggaa attttcccat cctcatagtc ctttatttgc aaagctagca
    13081 attccccata tctcaaacca gttaaatata gaaactcagc tattatgccg tgtagttttc
    13141 tgcgaggatt ggagtatagc tgtttcagta tttgatcaat ttcttctttc tccagatatt
    13201 ttttattcat agaaagcctt cttttttctt cttccacttt tttaggatgg attttaactg
    13261 ctagcgcagg gtttctttga atgtattttc tatcgattgc atagtttagc ataacagata
    13321 gagttgtttt tgtttgtttt gtgtagttca gtgagaggtc accaaacgta tacatatctt
    13381 caattatctt attaatgagt gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt
    13441 gcttagaaac atgtttcatc atcattggaa ccttcaaata gctagtacgt ttaacatgct
    13501 gcttataata ttcataccat tctttataca gctcaccaaa agtgatatct gatttattgt
    13561 aatcttcgag tgcttcttta atttttttat ctagaatttt ctgagctttt ttccacgctt
    13621 gtggtgaatt acttgtaagt gttgtagatt ttttccgtgt tttttctgta taaggatcta
    13681 catatctttc aataaacttg aatcgcccat ctttggtttg ttcaacccac acttttaaca
    13741 tctcctatca tttgctataa taggcataac aaatagacct atataggttt gttttctaaa
    13801 agcacgctct tactctggac ggtggggcgt gttttttagt gtgcttcaaa atctttattt
    13861 aatattctat ttaaaatcac attgaactct gtcgcgtcaa aatcacgcag taatataatt
    13921 tttccggatt ctctatacag gtgtgttcca tctgagaagg cctttatacc aactatatta
    13981 gaaaggttaa taactttcat tccattctcg ttaacaaaaa ttattctttt attagtcaaa
    14041 aatggtaccc caataaatac atcgttccat tctgttactt tgttcgaatt atgtttaatt
    14101 gatcccattc tataattcaa gcctttagca attctaatat tagaagataa gcctgaataa
    14161 ttagttcgaa ccgttctagt tttctgctct tgccatttga ctctctgttt aggtgtatag
    14221 taacaaaatt cattagattt tagattgacc ccatcaatta atgctgctct tttggataaa
    14281 tcctcttttt caagttgcct aatcattcta tttagttctt ctttttgctc tttttctgcc
    14341 agttcttttt cttctttttt tctttttgca attgttccat caaggtattc aactggtttt
    14401 aaacccataa aaattaaaaa aattccaagg ggtaaaaaaa caagtgtgac aggtaaaaca
    14461 atgaatgaca ttagacaaga taaaactcct ataataagtg caagatattt gagtattgtc
    14521 acaaaaaatt ttttcaaaga ttttcctcct ttgatataat agatttccca atcttaaaag
    14581 aggtcaggat agtccgtgtt gcggcacgag cttttttatt taacaagctc gctttgaaca
    14641 gtgaaatgta gaaatgtatt ttttctataa tcctaataat tcttttttct tcttttcaaa
    14701 ttcatctgta gtgatgatgt ccatatcaag caattctttt agttctttca ctttttggat
    14761 aggagaagtt aagctttgat tttgttcagt atgatcgtga tttttagctt ccattttttc
    14821 tttaacaaag tttacaaagg aatttatatc tccaatgctt atccatttca tatttacttt
    14881 atttcctgaa gcatagaaag agattttata tcccatagct tttttagcaa attcaaaaga
    14941 ggaaatattg gaatagggga aagattcact atcaaaaccg aaagttcttt taccaaaaaa
    15001 gaaaagtctt ttattcgtcg ctaaaaatac cccattcttt attgtatctg tacccatgga
    15061 ttttgtttca tacgttccaa atacaccttc aaccactttt tctccgggtt ctagatattc
    15121 ttgagcatat ttcaattgct tggaaaattt tttgtttttt gtatctagtg tcattttaaa
    15181 ctcctcattt ctatggtatt atttatgatg tagaatctta gaaataaggt ttttgtgtcc
    15241 gtgttgcagc acgggctttt tttaatgtat tactgaaaac gatttcttta ataattcttg
    15301 atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc attggcagac
    15361 agcataacaa gtatatctga gcttattctg aatagaataa tattccataa atttttcgag
    15421 attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata gaattagaat
    15481 agctacttta tctgcttctg tctcaaattt tgagtgaaaa gttgtagaag tatcgtacaa
    15541 aactgaaaat tcaaaatgcg aagcggtgaa atgcgcaagt tcatgagata aatgaaaggc
    15601 ttctgcagtt ttgccatata gattttcatt caaaaaaatg attctaggct tcggataata
    15661 gaagccaggc tctttcattt ccatgtaaac tactttcaaa ttgtattcgc tcaacatttc
    15721 tttcaacttt aaatacatac aaaccatcac tccaactatt cattttcttc tagggcttta
    15781 gcaattgcaa tagccttacg cattgtctct ttagatattt cttttccgtc aaaagagaat
    15841 acagtatcgt cttctgataa atccacatgt ttaggggcct cttttttctc tcttcctaat
    15901 agatagtcta cagagacatc gaaatagtca gcaatttctt gcaatttttc agcggatggt
    15961 tgttttccac tttttaaact atagaaatag ttttcactgt atcctaaatc aattgttact
    16021 tgtttcattg tttttgaatg tttttttgca agaaatttta tccgctcaaa tactgtcata
    16081 ccagcattct ccttttttct ttacaaaaaa ccaataaaaa agtgtagttt tgcgttgaca
    16141 taaacaacac tatagtgtat attggttttg taagttaatt ggatagaaaa aaagcaaagt
    16201 aaaaacacac cttatagcat taagtttggc gaccgagtgc gataaaaagg cttgttatat
    16261 gcttatttaa ctatgactat atactacact atagtatagt ttgcagtcaa ctaaaaatat
    16321 acttttctat ccaattttct ttctaaataa aaagaaagga agtgtgtgaa gtgagtaata
    16381 tcgataatgg gcgggaagcc atcaaagaat ttatgaaagc aaataatatt tcagaatacg
    16441 atttggccac tgcatatggt agatcgagaa cttggattca gcgtgtttta agtggaaaag
    16501 ataaaggtcc agctgttaac gcctttattc tggaagttat tcgcgatcat aaaattcgat
    16561 aggaggggga acaaaaatga aagaactaat caaagtaaca acaaacgaga acaacgaaca
    16621 gttagtaagc ggtagagagc tgcatgaatt tttggaggtg gcaacagaat acaaaaaatg
    16681 gtttagccgt atggcagaat atggttttgt tgaaaatata gatttcgtaa gggtgaccca
    16741 aaaatgtccg acccctggag gaattcagaa tattaccgac catgcaatga aattggacat
    16801 ggcaaaagaa atctcaatga tccaacgaac agaaaaaggt aaacaagcgc gtcaatattt
    16861 tcttcaagtg gaaaaagcat ggaatagcga agaaatgatc ttaatgcgaa gtcggcaaat
    16921 cctcgaaaga aaggtagaga cgttacaact tgaaaatgaa gaaatgaagc ccaaagcatt
    16981 atttgctgat tcagttagtg cgagtcacac gagtatttta gttggtgaat tagcaaaact
    17041 cataaagcaa aacggtgttg atattggttc taaacgactg tttagttggc tacgtgaaaa
    17101 ggaatattta atcaaacgta aaggaactga ttggaatatg ccaactcaaa aagcgatgga
    17161 tctaggacta tttgaaataa aagagacaac aatttctcat tctgatggtc atatttctat
    17221 taataaaact ccaaaagtca caggcaaagg acaagtatat tttgttaata aatttttagg
    17281 aggggtaata agtgaaaaaa ccaacacttt cagagttgat agaagctgct gagaaggcag
    17341 taaaaccaga cgactggtac cgacaaagtt taatcttgga gaagttccac ggcatgtcaa
    17401 aaactacttt agttgaatac tgcaaggaaa tggaaacaat tcctgaattt tcagaaggaa
    17461 ttgttcgtcc aggacattca accacattta ttcattatca tacttttatt tggtttttaa
    17521 aatggaaaga cgcaaataaa tatcgtgtaa aaatattatc tccttcagat gttttgaagg
    17581 aagcaagttg attattttca gagtaaaaag taaacaaaaa tattaggagg aaaatttgat
    17641 gaagattaca gtaccagatg aattgatagc agatgagttg acagaacaaa tagtaagaaa
    17701 ggttttagat gcacttgatg aacgattgaa ggtaatgaac aagtcagtgg agcttcctcc
    17761 ttatccaaac aaatcagagg tgaaaaaaat tttaggcatt ggtgatgaca aattaacaca
    17821 ttggataaac ctaggcttaa aaacacagca gtggagcaag ttagacatca gaattgaacg
    17881 atcggaactc caaagatttt tgaaagaaaa ctttgagttc taaaggcaaa ggagaacgat
    17941 tttatgtcct acacattgca acaagaacat caaattctcg gtttgattaa acaacgcagg
    18001 aaacaattac aagatgaccg tgcagtgctt agaaaagccg atgagctatc agataggcaa
    18061 gctgaactaa ttgcttctga acttgaggat ttgagaatgc tagaaataaa aaatagggag
    18121 attagattat gaagaagaca gacacacttt ttataggatt cattttgggg ttattagtga
    18181 ttgtagcgca ccaaagtatt atcgggggaa gcttgttcgc agcattgatg gttttaatca
    18241 atctgcttga ttcaaaagaa aggagcaact atggcacgag aagaagcgct aaaaatcggt
    18301 aaagtgattg ctgataattg gtgggcaaat agccgtccta ttattttaag caagcaacat
    18361 atcgaaaaga aaaaagcatg gcaacaaata aaaaagtgac tcagccgacc aaagcaatga
    18421 gtcacaaaga aaatacatct aaggagatgt taccacatgg aaaaagaact ttccactcta
    18481 gatcaatatt tgactgatcc tagttggggc aaatcgaata tcaaggaaac aagcaatcga
    18541 aaaatcagac gtaatctttt gacgaatgaa gaactagcat gtgatcaaga tgatttggga
    18601 aattttgtga gtatttggga tcatgtctat cttatccatt tatcgaagca gtccagaaaa
    18661 cctgaataca ttta
//
LOCUS       DACERL010000058        17819 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.062, whole genome shotgun sequence.
ACCESSION   DACERL010000058 DACERL010000000
VERSION     DACERL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17819
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.062"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="IXF54_002054"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="IXF54_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1405292.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="IXF54_002055"
     CDS             766..2139
                     /locus_tag="IXF54_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1405293.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="IXF54_002056"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="IXF54_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1405294.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="IXF54_002057"
     CDS             3194..3634
                     /locus_tag="IXF54_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1405295.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="IXF54_002058"
     CDS             3778..4479
                     /locus_tag="IXF54_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1405296.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="IXF54_002059"
     CDS             4476..5597
                     /locus_tag="IXF54_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1405297.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="IXF54_002060"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="IXF54_002060"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1405298.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="IXF54_002061"
     CDS             6976..7884
                     /locus_tag="IXF54_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1405299.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="IXF54_002062"
     CDS             7919..8191
                     /locus_tag="IXF54_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1405300.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="IXF54_002063"
     CDS             8202..9248
                     /locus_tag="IXF54_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1405301.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="IXF54_002064"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="IXF54_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1405302.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12930)
                     /locus_tag="IXF54_002065"
     CDS             complement(12259..12930)
                     /locus_tag="IXF54_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405303.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(12923..13417)
                     /locus_tag="IXF54_002066"
     CDS             complement(12923..13417)
                     /locus_tag="IXF54_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1405304.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="IXF54_002067"
     CDS             complement(13437..14657)
                     /locus_tag="IXF54_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405305.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="IXF54_002068"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="IXF54_002068"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1405306.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(16733..17815)
                     /locus_tag="IXF54_002069"
     CDS             complement(16733..17815)
                     /locus_tag="IXF54_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405307.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         5873 a         3221 c         3734 g         4991 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga
    16621 ggacgaaccg aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt
    16681 tacatggatt tgccaccggt ttgccaccgg tggttttttt cggtggcaaa aatcaagaat
    16741 tctttctaag attttccatg atatcgaccg tttcatttct catcttatct gttacatgcg
    16801 agtaagtatc catcgtaatt gaaattctgc tgtggcccaa tcgttcagag atttctttca
    16861 tttttgcgcc attttcgaga agaagtgtag catgagtatg tctgagagaa tggaagttaa
    16921 aagagaggga gagtgcattc gatatcctcc tagtattcca tttcactaca cttggcgtaa
    16981 ctagctcacc gtcttccttc gtacatactg catttgaatc aatgtagagc tttccatact
    17041 tcattcgatt ttctaattgt tgtttcttat gttttttcag aattgctagc aaggtttgtc
    17101 caataaaaat cgttcgattg gaactgcttg tctttggcgt accatatacc catgcgccgt
    17161 cattctttac catttgtttc tctacagtaa ttgttccatt tgaaaaatcg acatcatccc
    17221 acgtcagacc acaaacttcg ccaacgcgca ttcccgtata aaatccaata ttcaaaggaa
    17281 tatagaaagg atgtccttca ggagtaattt ctagcatatg atcaaagtcc tcaagagaaa
    17341 tgatttttag atctttttta gtcgttggtc gtgcttcata ttttggtatt tttacataca
    17401 gcataggatt ttgcttgatt aatccccaag gataaaccgc catattcagc gcattcttaa
    17461 ggacagagtg agtaatagtc attgttttct tcgagtaacc ctttttaaat tcagcattga
    17521 tgaaattttg taaaagagca ggggagagat ccgtaagttt tttctttcct aaataaccgt
    17581 ttatatgatt tttgatggta aatcggtagt tttcataggt attgtatttt agatttagtt
    17641 taacgtattc ctccatccaa aaatcaaggt attgttttac tcgagtatcc gtacctaaaa
    17701 agtattgtcc tgtttcgtca atatctgata aaacttttcg taaagcagct tcggcctctg
    17761 gtcgggtgtc tccgccaact ttctccactt tttttcttga gccatcatca ttgatatct
//
LOCUS       DACERL010000059        17427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.063, whole genome shotgun sequence.
ACCESSION   DACERL010000059 DACERL010000000
VERSION     DACERL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17427
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.063"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(5..1786)
                     /locus_tag="IXF54_002070"
     CDS             complement(5..1786)
                     /locus_tag="IXF54_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1405308.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(1812..2726)
                     /locus_tag="IXF54_002071"
     CDS             complement(1812..2726)
                     /locus_tag="IXF54_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405309.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(2753..3715)
                     /locus_tag="IXF54_002072"
     CDS             complement(2753..3715)
                     /locus_tag="IXF54_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405310.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(3715..4659)
                     /locus_tag="IXF54_002073"
     CDS             complement(3715..4659)
                     /locus_tag="IXF54_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405311.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(4660..5664)
                     /locus_tag="IXF54_002074"
     CDS             complement(4660..5664)
                     /locus_tag="IXF54_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405312.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(5932..6174)
                     /gene="acpP"
                     /locus_tag="IXF54_002075"
     CDS             complement(5932..6174)
                     /gene="acpP"
                     /locus_tag="IXF54_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1405313.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(6296..7297)
                     /gene="plsX"
                     /locus_tag="IXF54_002076"
     CDS             complement(6296..7297)
                     /gene="plsX"
                     /locus_tag="IXF54_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1405314.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(7346..9382)
                     /gene="recG"
                     /locus_tag="IXF54_002077"
     CDS             complement(7346..9382)
                     /gene="recG"
                     /locus_tag="IXF54_002077"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1405315.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(9499..11181)
                     /locus_tag="IXF54_002078"
     CDS             complement(9499..11181)
                     /locus_tag="IXF54_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1405316.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(11233..11595)
                     /locus_tag="IXF54_002079"
     CDS             complement(11233..11595)
                     /locus_tag="IXF54_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1405317.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            11865..12053
                     /locus_tag="IXF54_002080"
     CDS             11865..12053
                     /locus_tag="IXF54_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1405318.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            12291..14960
                     /locus_tag="IXF54_002081"
     CDS             12291..14960
                     /locus_tag="IXF54_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1405319.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGSASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(15051..15671)
                     /locus_tag="IXF54_002082"
     CDS             complement(15051..15671)
                     /locus_tag="IXF54_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1405320.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            15784..16323
                     /locus_tag="IXF54_002083"
     CDS             15784..16323
                     /locus_tag="IXF54_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405321.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            16320..16658
                     /locus_tag="IXF54_002084"
     CDS             16320..16658
                     /locus_tag="IXF54_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1405322.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(17039..>17427)
                     /locus_tag="IXF54_002085"
     CDS             complement(17039..>17427)
                     /locus_tag="IXF54_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1405323.1"
                     /translation="NIIEELSRNSRITMKELGEKVHLTGPAVSARVTKLEESGVIENY
                     TVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGCYLLECRF
                     TSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         4914 a         3600 c         3125 g         5788 t
ORIGIN      
        1 gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt ggtacatcat
       61 aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta cggaacaacg
      121 ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat gcttcttttt
      181 ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt gtattttctt
      241 ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca cttggatttg
      301 gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca tcgttttcta
      361 atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg acatccaaac
      421 cgatttcttt ccactgttgg atatagtaat ctgccaatgg ttgcgctgtt tccccaccgg
      481 acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt aaaccatcgt
      541 catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct agatcttgtg
      601 tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc aatgaagtcg
      661 cattgcttct caaacctgca tagaaacgct cacctactgc attattatct aatgcatagc
      721 ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat tccacactct
      781 ttgtatctgc attccattta cctaacttga atcctagata agtataagaa gtttgttcac
      841 ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct gttggcattt
      901 ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc atcggtacag
      961 atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg tttggcaagt
     1021 attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa gtcactggat
     1081 ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct aacgtatgtt
     1141 ttggtgctgc atatgaccaa ataccaccac ccgattgaag catgcttggg tttacttctt
     1201 tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt gtgtcggcac
     1261 ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca taacggatac
     1321 cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc gccgttacat
     1381 cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt ttcgtatctt
     1441 catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca tcgtttgcaa
     1501 atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg taaaactccc
     1561 actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat tcgccgtctt
     1621 cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct ttgttattgc
     1681 cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat gctgcaagaa
     1741 cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct atttccccct
     1801 tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa tgcttgtcca
     1861 acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa ccagatccat
     1921 tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc taaactaggt
     1981 acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc gatgtttgcc
     2041 gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat gatttcgcgg
     2101 aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata atctcttctt
     2161 gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata aaatgcactc
     2221 atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa gacgataatg
     2281 atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat gttgtcaatg
     2341 atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga agtaaggatc
     2401 gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg tcctaataca
     2461 tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga aactgttcct
     2521 ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc ggtcagcatc
     2581 gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa aatagcaatt
     2641 ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat actttcttga
     2701 actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg ttttattgaa
     2761 tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc cccactagcg
     2821 tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt tggccgatgg
     2881 aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata actacagaac
     2941 cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac gctgcatttc
     3001 ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg gctgttctga
     3061 caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt gtgacggcag
     3121 tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg tctatccatt
     3181 gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg aaccagccta
     3241 aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga acagcaaaac
     3301 taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac cggccagcaa
     3361 gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc gatagatata
     3421 aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat aagaaacttt
     3481 gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca taccatgggt
     3541 cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga tttggattga
     3601 tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc agaacactta
     3661 aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc cacattagct
     3721 tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca aatcgtagac
     3781 tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt ctactcttcg
     3841 tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag ctgaaagcag
     3901 acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac cgatttctac
     3961 aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta cacctaaatc
     4021 atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca tgaaattcaa
     4081 tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta cgatcagttt
     4141 tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc ctgaaaattc
     4201 atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca gtccttttac
     4261 ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt cagcaatgat
     4321 atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga tcatctgcac
     4381 atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt ttccttcata
     4441 catgatttgt ccggctgtca ccttttctaa tcccacaacg gcttttcccg tagtagattt
     4501 tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa taaaatccac
     4561 accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc gaatagggta
     4621 atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc ctttttcgtc
     4681 tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg cgacttcatg
     4741 aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg gtgcaaaccg
     4801 acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca catggagttc
     4861 tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat aaggatgctt
     4921 aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc catacatcac
     4981 tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca agatgatccc
     5041 tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt ggatggttac
     5101 atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg cgattgcgat
     5161 tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt ggcgtccaac
     5221 tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag cttgacgctg
     5281 ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt cgattctcat
     5341 gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat ttccgcggat
     5401 tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata aaatctcacc
     5461 tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca aggtactttt
     5521 cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag taaatgaaac
     5581 gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta ggttctgaac
     5641 ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa tggaagtttt
     5701 tcttaataaa aaaggacatt acacagaata aagcgtttta taacaaaggt tattcttatc
     5761 tttgtgtaat gaattattaa taattatata gaaaatcgct ttactttgca acataatttc
     5821 tgtttatcgc cagaattatc agaaaaatga cttttacgtg tcaaaaagat taaaaaaaga
     5881 aggataaaaa gctgtatgca gctttttatc cttctttgct tttatttgaa attatttaag
     5941 atggctgctg atataatcta ctgcgccacc gactgtttca atttgttcag cgtcttcatc
     6001 tgaaatttcc gtaccgaatt catcttcaag ctctaaaacg aattccataa tactaattga
     6061 atcagctttt aaatcatctt tgatgttcaa ttcatccgtc actttatcgg tatctatctc
     6121 aaaatgattt gaaataattt tcgctacttt gttaaatact tcttcgcgag tcaacttttt
     6181 cacctccaag aatcatacac ttgtcaatta cctacaccat ggctaatttt actggcattt
     6241 ttcaggaatg tccacctttt tttcaatgga acggattgct tcagatatcc aaatactatt
     6301 cttcgctttt ttcaaattgt tcgactaatt gtccaactac atctgtctca agcattgtgt
     6361 ggatttggcg gatcgtatag cgaacagcat cggggcctgt tgctccatga gttttaatga
     6421 ctggtgcttt caagccaaac aaaacggcac cgccatgttt ggaataatcc atttctgctt
     6481 tcaaagaacg taagccgtct tttaaaagca tagcgcccat ttttcctttg atcccttcat
     6541 ttagaatagc agattttaat agattcatca tattcattgc tgttccttca atagatttca
     6601 aaacagcgtt gcctgtaaat ccatctgtga ccactacatc agccacacca ttgagtagct
     6661 cacgagcttc tacgtttcca ataaaattca agcttgtttc attctgaagg agttcatagg
     6721 cttttttcgt cacttcacta ccttttgttg cttctgttcc gttgtttaag agtgcgacac
     6781 gaggacgtcc gatacctcgg actttacgtg cataaaaaga acctaaaata ccgtattgca
     6841 acaaatgttc tggtttgttt tctgcgttag cgccaagatc cagcatatcg aaaccggcac
     6901 cttctttccc tactactggc aaggtagaca tcaagccagg gcgttcgata tttttgatac
     6961 gtcctacgat gaacaaccct gctgctagta gcgcgcctgt attccctgct gaaaatacag
     7021 catctgcttc accgtttttc actgcttgtg cagcaaggac cattgatgca gtcttttttc
     7081 gtcgaattgc ttttactggc tcatcatcac tattgatttt ttcgtctgta tggataatcg
     7141 tgatgttttt ttcatttgtt aagtatttct tgatttctgc ttcttttccg taaagtaaga
     7201 attctacttc tggaaaatct tgctgcgcta aagccacgcc ttctacaatt gcttgaggtg
     7261 cgtgatctcc gcccattgca tctacggcaa ttttcattgt ttccactcct ttttccgtca
     7321 tcagtataaa gtatagcaca gtctgctaat cgaaataacg attatcctga ttttctattt
     7381 ggaggctttg cgctaaaggc tgatactcag gaagcatctg ccaatccttt atctgccaaa
     7441 gggctgcagc ctcttctctg gctacctcca aaacatttcc atccgtcacc aagtcagcaa
     7501 tcgcaaattg aggcagacca gattgacgga agccaaatac ttcaccagga ccacgtaatt
     7561 ccagatcttt ttcgcttagt acaaaaccat tggtggtctc tgtcatgatt ttcatccgtt
     7621 ccacacctag ttcattttta ggattagcaa ctaagataca gtaggaagca tcacttccgc
     7681 gtccaacacg tccacgaagt tgatgcagct gtgccaatcc aaatcgatcc gcatccatga
     7741 tcagcatgac ggtcgcgttc ggcacgttta cccctacttc aattacagta gtcgaaacta
     7801 atatctgcat ctggttgtct ttgaaggctt ccatgattac ttctttttcc tgattcttca
     7861 ttttaccatg taacaagcca acttcatatt ttggtgcaaa tagttcacga agtttttcat
     7921 agatttccac tgcatttttt acatctaatg cttctgattc ttcaatcagc ggacagatga
     7981 catacatttg atgtcctctt gccagttctt ttttcgtcca atccagtaca ctgtccaatt
     8041 gaggtgttct tacccagcgt gtttcgatcg ggatacgtcc agctggaagt tcatcaatga
     8101 tagatacatc catctcacca tatgctgtaa tagctaatgt tctaggaatc ggagtcgctg
     8161 tcataaataa gacatctggt cgccagcctt tttcacgaag tactttccgc tggttcacgc
     8221 caaaacggtg ctgctcatcc gtaataacta gacctaaatt ctgaaattcg actccatctt
     8281 ggatcaatgc atgtgtgccg atcacgatgt taatttcccc ttgctctagt ttttcaagga
     8341 gctcccgacg ttcttttgct ttcgttgacc ccgtaagcaa tgcgatggtt acttcattgg
     8401 gatcaaacag ctgttgcaag ctttccatat gttgttgtgc caagatttct gtaggcacca
     8461 tcaacgcacc ttggaaacct gctgtaacgg tagcgtataa cgcaatcgcg gcaaccactg
     8521 tttttccgct acctacatct ccctgcaaaa gtcgctgcat atgctgcgga ctgcgaagat
     8581 cccgacagat ttcattcgtt acacgttttt gtgcatttgt cagttcaaaa ggtagctttt
     8641 gagtaaattc tttcaatttt tggacatcat aaaggatctc caaaccattc gtttctgctt
     8701 tttctgcttt tttcaatcct tgaagacgca tttgaaataa aaagaactct tcaaaaacga
     8761 cacgtcgttt ggcttgatgg ttttcttcgt ggtcttttgg aaaatgcata cttcttacag
     8821 cagaagctcg gtcaagcagt cgatatttct ccacaaggtt gtttggcaga ttttcttcaa
     8881 tcaatgtgcc gtattcttga aacccttttt tgattagatc aactaatgtc gtttgacgaa
     8941 ttgatttgtt gacgtgatat atcggcgaaa agtcatccat tgtttgagaa cctaaaatct
     9001 tcatcccgtt caatgcttta cgctttgcat cccatttacc gtatacggcg atttcctccg
     9061 ataaaatgac tttttctttt agataaggtt gattaaaaaa agaaacgttg aacacatcat
     9121 gttcttgcat catacgaaat tgcaatcggc tttttttata accaaatcga ttcatcacag
     9181 gaggcgagac tacgattcct ttcaaagtaa ccttttcttg atcttggatc tcgtttagat
     9241 ttctttcctg aatatcttcg tagcggaaag gataataagt cagtaagtct tcaatcgttt
     9301 ggatgcccaa gctggcaaga ctagctgctc ttttttctcc gactcctgcc agcatagtga
     9361 ttggatcatc taaagaacgc ataaattttt cctccctcct ttaacaaaaa aaggacttgg
     9421 tcagcgatca tacctgatca ctaaccgaag gccttttatg aatgaatact gtgttagaaa
     9481 gatattctga cacagaaatt attcagcaga aaatagataa ggatagacag gctgatctcc
     9541 ttggtggatc tcgatttcca aatcaggatg tttttctgtt agtttctcag caaattcatt
     9601 tgcttgttca actgttcctt catcacctgc caagatcgtt acgatctctg tgtcctcatt
     9661 gatcatttgt tctagtgtac ttaacgctgt tgctgtgata tctgggttag agatcaggat
     9721 ttttccatcg atcatgccca aaaagtctcc ttctttgatc tctacaccgt caattgaggt
     9781 atctctgatc gcgtgagtga cttgaccgct cacaacgcta gacaacattt ctgtcatcgt
     9841 ttctttgttt tcttctaaag aagcttgatc attgaatgaa agcatagctg tcaatccttg
     9901 ggaaatcgtt cttgaaggaa caactgctac tgggatatct gctacttctg ctgcttgatc
     9961 agctgccatg aagatgttct tattgtttgg caagacaatc actttatctg cgtttacttc
    10021 ttgaatcgct ttaacaatgt cttccgtact tggattcatt gtttgcccac cactgataac
    10081 ataattagcg cctaaacttt caaacagctc ttggacgcct ttaccagcag cgatcgctac
    10141 gataccatac gggatacgtg gtgtttcttt tttcgcaaac tcgttcactt cttcatcgtg
    10201 ttccaaaatc gtctcatgct gcaagcgcat gttgtcgact ttgattttga ctaatgaacc
    10261 gaatttttgt ccataattca agacttctcc tgggttttct gtatgcacgt ggactttgat
    10321 gatctcgtca tcattgacca caagtaaaga atctcctata ccatctaaat agttacggaa
    10381 ttcttcgtaa tcgaatgtac tatcgactgt aggtccttct ccgattttta ccatgatttc
    10441 tgtacagtaa ccaaaatgga tatcttctgt cgccatttga ccttggacac tgcgatgatg
    10501 ttctgcattg accatatcat ccatttctgc tggagaaggt tcatatgttt catccgcttg
    10561 gaattctcca ttcaatgcac ttaaaaagcc ctcataaaca aacagcagtc cttgccctcc
    10621 actgtctacg acaccgactt cttttaggac tggaagcaag tctggagttt tagctaaagc
    10681 acgctttgcc ccttttacaa cagctgtcat tacttcaacg acatcatcag tactttttgc
    10741 tttcttttct ccagctttcg ctgcttctct agctactgtc aaaatcgttc cttctactgg
    10801 tttcattact gctttatacg cagtttcaac tccatgagtg aaggctgcag caagatcgga
    10861 agcatttaat gagacaacat ctggaatttg cttagaaaat ccacgaaaca attgagacaa
    10921 aataacgccg gaatttccgc gcgcacccat cagcaatcct tttgaaagca ttgctgctaa
    10981 ttctcctact ttttctgagg ttgaatctac tactgcctta gctccactag tcatagatag
    11041 attcatattt gttcccgtat cgccatctgg cacagggaat acatttaatg aattgacgta
    11101 ttctgcattt acttgcagac gattcgcacc tgcctggacc atttcctgga actggcttgc
    11161 acttatttct gttacactca cctagataat cctccttcag atcgactgct gagttaccag
    11221 ccagtctcct gcctagtctg gcagtacacg aacaccttgc acaaagacat taactgaatt
    11281 ggcagttacg cctaataatg cctctagatt gtatttcact ttttcttgga cgttgcggga
    11341 aacttcagag attttcgtac catagctgat aatcgtataa acatctacag caataccatt
    11401 atcttcttgg cggacaacta ctcctcttga gtaattttct ttacgcaaaa tctcagtaat
    11461 attatcttta atttgatttt tgctggccat tcctacaaca ccgaacacat ctgtcgcggc
    11521 gccaccaaca actgttgcaa ttacatcatt ggtaatttca atggttccag ctgacgtttg
    11581 aatttttaca gccatgaatg agcctcctta acgggtaaaa aactcccatt tttcttctat
    11641 catattttat catagcgcaa cttgaaataa aaggcagtta cccaaagtac tcaaaaacct
    11701 tgcaaaattt caggtataac cttgtttaat ttacaattat tcttctcaat tgtcaattgt
    11761 atctatgaaa atgtacttgc aaatttccat tctatatgat atgttagtct ggtatgagct
    11821 gaattattct attgctccaa atcaaggcaa aggaggaaat taccatggca aaagtttgtt
    11881 actttacagg tcgcaagaca aaaagtggaa acaaccgttc acacgctatg aactcaacaa
    11941 aacgtactgt taaacctaac ttacaaaaag ttcgcgttat ggtcgacgga aaacctaaaa
    12001 aagtttgggt gtcaactcgt gctttgaaat caggaaaagt tgaacgcgtt taattgaatg
    12061 atacttggac cgttcagata gtgaacggtt ttttatttaa taaaaatttt taagggtggg
    12121 acttttgatt cctacccttt ttttgcgttt atttttgtta tacgaacaaa ttatgaagtt
    12181 atttattcta aattttattt ttttgtgaaa actagactct ttttaagcag agaaaaacaa
    12241 ggatatgata aactgaatac aacttcatag aaatttggtg gaaaataaat atgaaaaaaa
    12301 atttaaagtc attctttaga gaaaacggac gctacgcagc agctagtttc ctgattccgt
    12361 ttttgatttt agcacttgtt tatctaagta tcgggatcta tcctggaagc agcagaagta
    12421 ttttggcaag tgatgccttt tcacagtttt ctaattttca tgccagcttc cgaaatgttc
    12481 ttcttggaaa gcaaagtatt ttttatacat ggaatgcttc tctaggattg aattatttat
    12541 ctttaatttc ttactatcta ggcgggatct tcacgccact ggtgttcttt tttccaaacc
    12601 agatgatgcc agatgcactg tatgttctga ctttgcttaa ggtcggctgt gccggattgg
    12661 ctttttggtt tcttgcacgt acgttcaaga ttcctcgctg ggggcatgtt gcacttagcg
    12721 tcagttacgc attgatctca tttatcactg cacattcgga gatcatcatg tggctggatg
    12781 catttgttta tcttccatta gtgattttgg ggattcatcg tgttatggat cttaaacggc
    12841 cgaaactttt atttatcagt tatttactct tgtttttatc cagtttttat atgggtttta
    12901 tgattggcgt attttctttc ttatatttta tcgcccgtct attgacaaac tggtcagaat
    12961 ataaaaaaac gattgttcct tatggtatca cttctttact ggcaggcagc gcttcaatga
    13021 tcattgtatt gccagcagtg ctggatcttc ggtcaaatgg cgaggcactg agtcagatca
    13081 ctacgttgaa aacagaagcg acttcttatc tggatctaat catgaaaaat atgattggtg
    13141 tttatgacac gacaaaatac ggatcgattc cgttcgttta cgcaggtctg ctaccattag
    13201 tcctctgtct gttttatttt gccagtcgga aagtaccgtt gaaaaataaa tttttgtacg
    13261 gcagtctttt cgccatactt atcgcaagtt tctatcttgt tccattgaat ttgttctggc
    13321 atggtatgca tgcaccgaat atgtttttat tccgttatgc ctatctgttc tctttcttag
    13381 tgctcctgct tgcaggaaaa ggatgggaaa agatagaaca agacgatcgt gggctgttgt
    13441 taggaatcat tttggtcgta gctgctctct ttgcggtggc atggggcatc aaacctgaag
    13501 gaagctacac ctatgtgacg atgacttcct ttgtattgac agtggttttc ttagggattt
    13561 atgcttttgc tgtgggattt tatcagcata aaaaactttc attcaatcat ctgagtatat
    13621 tgttattatt gatcatgtct gctgaagcac tggtcaatac gaacagtatg atccatggca
    13681 ttcttgatga ttggaattat gcttcccgca gtttatatac tgatccttat cctgacttga
    13741 aagctcttgt aaatgaaaca aaaaaagatt ccgactcgtt ttaccgcttg gaaaatctgg
    13801 atccagtatc tgcaaacgat tcgatcaact atggttacag tggtatcagt ttgttttctt
    13861 ctataagaaa tcgtaattct tcctcttacc tggataaatt aggtttccgt tcaagaggga
    13921 caaatttgaa tatccgttat aacaataata ctttgctaat ggatgggttt acaggaatca
    13981 aatataacat ttctaaaaac gaaagcacgt tcagtaaata tggcttccgc caagctggtc
    14041 aaagtggcga ctatacactg tatcagaatg tcaatgcatt gccactagca tttactgcac
    14101 cgttgtccgt taatgatgtt gagcagccag tgacagataa tttgaccagt cagacaaact
    14161 tgatcaatac attgtctggt ttaaatgaac agtattttac attctataca cctactttga
    14221 agaatcagtt gaatacgacg atcacaaaga caacgactgg cgtcacttac tcagaaacag
    14281 cagataacca gccaaaagag ctcacttggg aagtccatgt gcctgcaaat acacaagctt
    14341 acttaagttt gttcccaacc aattttgcac aattagaaag ttctagtgcg acgatcactg
    14401 tcaatggtac atcgcgaaaa acgcaaatca acatttctgg tcaatactat gatcttggct
    14461 attatcctca agagacaaca gtgacattta ctgcaagttt ttatggaacg aaagaagtaa
    14521 gtttcatgga gcctaaagtc gttggtctag atgttgtagc ctatcaagcc gcaatgaatc
    14581 agatcaaaga aaaaggtgtc gaaatggaag ccactggcag aaaagcttct gggactgtaa
    14641 ataccgctga agattccatg ttggtgacaa ctattcctta tgacaaagga tggaaagcta
    14701 aaattgatgg gaaaccagtc aaggtcagca atttcaaaga cgcattcttg atgataaaag
    14761 ttccagcagg taaacatact gtcactttca gttacttgcc tgaaggattt acgataggtg
    14821 caatcctttt tgtcctatgt attgcattgt ttattcttta tgtctggtac cttgaccgca
    14881 atcgcccctt ttatggcacg attgatccag caatagaaga aaaaccgcga aatacacaca
    14941 gaagaagacg aagaaaataa agaaacgatg gaatctgtgt cactcttcta tttaaatggc
    15001 aaaaatttca atctttaaat gacaaaaacc acctgatttt tggtggtttt ttatatacct
    15061 atgactaaac tatctcttgc tgcttcgaca acttctacag tgatcttatc catttgattg
    15121 attcttaaaa tccgatcaat ctgcgcaaat aaacgatgaa taagcctaaa atttcctttt
    15181 gtgatcttaa tgatagttgt gatagcctca taatcagaaa aatcttctag cttgatatca
    15241 attcctaaat ctgcccattt gtattccaaa atatgatgtg tttcgtcctt actaaggtta
    15301 tcaaattcat gtgcaaatcc tatgcgagag taaagctgcg gataacgaga caatcttttt
    15361 tcaataccag gcataccgat aagaaccatt gccagatcat ttctatcgta aatatctctt
    15421 agctgttcta aatgctgaag ctttaaacga tcaatttcat cgacaataat taagtcaatt
    15481 gcttcatagg ttttaggcac aggaggatct tcttgattgt attttagtac catatgcatt
    15541 gctttagtca gacctattct actagttatt gtgttaatat ccgaactgac acgagacatc
    15601 ttttcagctg gtacagtata aaaaacttta tttacactta atatttcatc tgttgcattt
    15661 atcaagaaca agaaaaagtc tgaatattca aaaaaataac ggtgtacatt tttaaaagga
    15721 gatgtataat cgagattgag aaagcgtttt cctcaaaatt taatgaatta ggaagtgatt
    15781 actatgaaaa aattatttag cgcacttttt attctgttat ttggttttat tggtattggg
    15841 acttctggaa atatagttaa agctagcacg atggttgaac aaacaagtga aatggcaaaa
    15901 acttttagtc aattaacacc agaagaacaa agttacttca aatccaaagg atttagtgat
    15961 aaagatactt tttacactca aaagatagag cctcaagatc cgaaaacacg tttttctgca
    16021 aatgtattgc ggttaaccgc atctactaaa aaaattacta atacgcaggg gcggacagaa
    16081 tatattataa caagtcccaa ttcggcgatg tatcgtattt ctacacgctt gagtttaggt
    16141 agtgcaaaat catatactca aacagtaaat ctagctaatt cttatggtta tggtggagga
    16201 atgtatttta catttactgg gaaaagacaa tattacagtg taaaattagc tatgcaagtt
    16261 tataatacat ttggtgtagg gtcagtctct tgcactgcgg gaggattgac aataggaaaa
    16321 tgataatttc ttactatgtc ttaaatagaa gtgatgatga aggactcttc gtttttcaat
    16381 cattacatga ggatacgatg ctaaaagagt tttataatcg gattaagaat aaacagatag
    16441 aaacaaattt atatttttta agtgactcta tcttgccttt tattagcaac cctaaaattc
    16501 ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat tattgtcatt gatgaggaaa
    16561 ttgtaaaata tggcgcttat ctaagcgtag aagaattaga aaaattaact aacattggaa
    16621 tgagtattca ggaggataat ggggtacatg gaacatagaa tgaatatgca cagttgagaa
    16681 cggaatttga catttattta gaccaacagg gtataccctg ttggtctttt tgtgcgttca
    16741 tgatatactt ggtgtcattt ttatatttaa atggcacaaa tatttctaat tatttgtcat
    16801 ttaacgctga aaatttttgc tatttatcta aaaaagtgac aggatactca tttggccttc
    16861 atagaatttt ctaaatatga atcatcaatg aatttaatta aaaaaataag tagggcaaaa
    16921 tataaatgtt tttgccttac ttgtttccat cagtttagtt ttaaattgat aagttatata
    16981 gttatggttg aatgtgtgtc tcgcatatta gtatttttac catctgctag ttttattatt
    17041 aatctataac aatagataat ttataattcg catattcatt aagctgttcc aaaaagctat
    17101 tcattgattt ttttgaagta aatctacatt ccaaaagata acagccccct ccactaatct
    17161 tataatttct aatcaagaat ttttcatgat ttttaataaa tgataaatag ggtttatgat
    17221 ttaaattttg cgtaattata attaaaaaag cgtgaatcga aaaccctagc ttttccatat
    17281 ttaattttac tgtataattt tcaataactc cactttcctc tagttttgtc actctcgccg
    17341 agacagcggg tccagtcaaa tgtacttttt ctccaagttc tttcattgtg attcgactat
    17401 ttcttgatag ttcctcaatg atattta
//
LOCUS       DACERL010000060        17107 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.064, whole genome shotgun sequence.
ACCESSION   DACERL010000060 DACERL010000000
VERSION     DACERL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.064"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            7..1398
                     /locus_tag="IXF54_002086"
     CDS             7..1398
                     /locus_tag="IXF54_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1405324.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IXF54_002087"
     CDS             1414..2454
                     /locus_tag="IXF54_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1405325.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IXF54_002088"
     CDS             2473..3558
                     /locus_tag="IXF54_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1405326.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IXF54_002089"
     CDS             3591..4553
                     /locus_tag="IXF54_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1405327.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="IXF54_002090"
     CDS             4546..5979
                     /locus_tag="IXF54_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1405328.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="IXF54_002091"
     CDS             5976..7046
                     /locus_tag="IXF54_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405329.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="IXF54_002092"
     CDS             7033..8454
                     /locus_tag="IXF54_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1405330.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="IXF54_002093"
     CDS             8467..9474
                     /locus_tag="IXF54_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1405331.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="IXF54_002094"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="IXF54_002094"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1405332.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="IXF54_002095"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="IXF54_002095"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1405333.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="IXF54_002096"
     CDS             11608..12285
                     /locus_tag="IXF54_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1405334.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="IXF54_002097"
     CDS             12275..13417
                     /locus_tag="IXF54_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1405335.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="IXF54_002098"
     CDS             13430..14407
                     /locus_tag="IXF54_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1405336.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="IXF54_002099"
     CDS             14407..15315
                     /locus_tag="IXF54_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1405337.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="IXF54_002100"
     CDS             15316..16068
                     /locus_tag="IXF54_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ1405338.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="IXF54_002101"
     CDS             16073..17020
                     /locus_tag="IXF54_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1405339.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DACERL010000061        17050 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.065, whole genome shotgun sequence.
ACCESSION   DACERL010000061 DACERL010000000
VERSION     DACERL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.065"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            83..970
                     /locus_tag="IXF54_002102"
     CDS             83..970
                     /locus_tag="IXF54_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1405340.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXF54_002103"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXF54_002103"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1405341.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXF54_002104"
     CDS             2272..2718
                     /locus_tag="IXF54_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1405342.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IXF54_002105"
     CDS             2991..4343
                     /locus_tag="IXF54_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1405343.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IXF54_002106"
     CDS             4514..4801
                     /locus_tag="IXF54_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405344.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IXF54_002107"
     CDS             5027..5470
                     /locus_tag="IXF54_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1405345.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IXF54_002108"
     CDS             complement(5476..6660)
                     /locus_tag="IXF54_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1405346.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IXF54_002109"
     CDS             6928..7626
                     /locus_tag="IXF54_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1405347.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IXF54_002110"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IXF54_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1405348.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IXF54_002111"
     CDS             complement(8436..8969)
                     /locus_tag="IXF54_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405349.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKLCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IXF54_002112"
     CDS             complement(9266..11647)
                     /locus_tag="IXF54_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1405350.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IXF54_002113"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IXF54_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1405351.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IXF54_002114"
     CDS             12348..12887
                     /locus_tag="IXF54_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1405352.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IXF54_002115"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IXF54_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1405353.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IXF54_002116"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXF54_002116"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IXF54_002117"
     CDS             13994..15160
                     /locus_tag="IXF54_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1405354.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IXF54_002118"
     CDS             15206..16702
                     /locus_tag="IXF54_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1405355.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..>17050
                     /locus_tag="IXF54_002119"
     CDS             16783..>17050
                     /locus_tag="IXF54_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1405356.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQIS"
BASE COUNT         5769 a         2931 c         3530 g         4820 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacata atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga
//
LOCUS       DACERL010000062        16528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.066, whole genome shotgun sequence.
ACCESSION   DACERL010000062 DACERL010000000
VERSION     DACERL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.066"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(142..417)
                     /locus_tag="IXF54_002120"
     CDS             complement(142..417)
                     /locus_tag="IXF54_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1405357.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IXF54_002121"
     CDS             complement(660..1970)
                     /locus_tag="IXF54_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1405358.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IXF54_002122"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IXF54_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1405359.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IXF54_002123"
     CDS             complement(3516..4196)
                     /locus_tag="IXF54_002123"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1405360.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IXF54_002124"
     CDS             complement(4245..4880)
                     /locus_tag="IXF54_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1405361.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IXF54_002125"
     CDS             complement(4946..6349)
                     /locus_tag="IXF54_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1405362.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IXF54_002126"
     CDS             complement(6336..7367)
                     /locus_tag="IXF54_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405363.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IXF54_002127"
     CDS             7398..7619
                     /locus_tag="IXF54_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1405364.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IXF54_002128"
     CDS             complement(7784..8521)
                     /locus_tag="IXF54_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1405365.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IXF54_002129"
     CDS             complement(8674..10014)
                     /locus_tag="IXF54_002129"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1405366.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IXF54_002130"
     CDS             complement(10251..11774)
                     /locus_tag="IXF54_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1405367.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IXF54_002131"
     CDS             complement(12152..12763)
                     /locus_tag="IXF54_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1405368.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IXF54_002132"
     CDS             complement(13093..13809)
                     /locus_tag="IXF54_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1405369.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IXF54_002133"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IXF54_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1405370.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IXF54_002134"
     CDS             complement(14368..15165)
                     /locus_tag="IXF54_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1405371.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IXF54_002135"
     CDS             complement(15135..15506)
                     /locus_tag="IXF54_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1405372.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IXF54_002136"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IXF54_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1405373.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5508 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttctttt
//
LOCUS       DACERL010000063        16399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.067, whole genome shotgun sequence.
ACCESSION   DACERL010000063 DACERL010000000
VERSION     DACERL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.067"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(296..1024)
                     /locus_tag="IXF54_002137"
     CDS             complement(296..1024)
                     /locus_tag="IXF54_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405374.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IXF54_002138"
     CDS             complement(1002..2576)
                     /locus_tag="IXF54_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1405375.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IXF54_002139"
     CDS             2805..3743
                     /locus_tag="IXF54_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1405376.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IXF54_002140"
     CDS             3755..4666
                     /locus_tag="IXF54_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1405377.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IXF54_002141"
     CDS             4690..6162
                     /locus_tag="IXF54_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1405378.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="IXF54_002142"
     CDS             6214..6825
                     /locus_tag="IXF54_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1405379.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="IXF54_002143"
     CDS             6855..8768
                     /locus_tag="IXF54_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ1405380.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="IXF54_002144"
     CDS             8896..11877
                     /locus_tag="IXF54_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ1405381.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="IXF54_002145"
     CDS             11874..13535
                     /locus_tag="IXF54_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ1405382.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="IXF54_002146"
     CDS             13575..14507
                     /locus_tag="IXF54_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1405383.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IXF54_002147"
     CDS             complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IXF54_002147"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1405384.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         5239 a         2689 c         3301 g         5170 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gataatttt
//
LOCUS       DACERL010000064        15860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.068, whole genome shotgun sequence.
ACCESSION   DACERL010000064 DACERL010000000
VERSION     DACERL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.068"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(12..482)
                     /locus_tag="IXF54_002148"
     CDS             complement(12..482)
                     /locus_tag="IXF54_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1405385.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IXF54_002149"
     CDS             complement(641..1099)
                     /locus_tag="IXF54_002149"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1405386.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IXF54_002150"
     CDS             complement(1270..2415)
                     /locus_tag="IXF54_002150"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1405387.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IXF54_002151"
     CDS             complement(2428..3345)
                     /locus_tag="IXF54_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1405388.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IXF54_002152"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IXF54_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1405389.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IXF54_002153"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IXF54_002153"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1405390.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IXF54_002154"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IXF54_002154"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1405391.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IXF54_002155"
     CDS             6871..7752
                     /locus_tag="IXF54_002155"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1405392.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IXF54_002156"
     CDS             7749..8465
                     /locus_tag="IXF54_002156"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1405393.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IXF54_002157"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IXF54_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1405394.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IXF54_002158"
     CDS             9182..10183
                     /locus_tag="IXF54_002158"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1405395.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IXF54_002159"
     CDS             10180..11541
                     /locus_tag="IXF54_002159"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1405396.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IXF54_002160"
     CDS             11501..12652
                     /locus_tag="IXF54_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1405397.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IXF54_002161"
     CDS             12627..13796
                     /locus_tag="IXF54_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1405398.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IXF54_002162"
     CDS             13971..15350
                     /locus_tag="IXF54_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1405399.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="IXF54_002163"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="IXF54_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5185 a         2799 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DACERL010000065        15313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.069, whole genome shotgun sequence.
ACCESSION   DACERL010000065 DACERL010000000
VERSION     DACERL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.069"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            278..787
                     /locus_tag="IXF54_002164"
     CDS             278..787
                     /locus_tag="IXF54_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1405400.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IXF54_002165"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IXF54_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1405401.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IXF54_002166"
     CDS             2322..3302
                     /locus_tag="IXF54_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1405402.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IXF54_002167"
     CDS             3424..4842
                     /locus_tag="IXF54_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1405403.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IXF54_002168"
     CDS             4913..6382
                     /locus_tag="IXF54_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1405404.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IXF54_002169"
     CDS             6392..6859
                     /locus_tag="IXF54_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1405405.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IXF54_002170"
     CDS             6895..7767
                     /locus_tag="IXF54_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1405406.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IXF54_002171"
     CDS             7849..10383
                     /locus_tag="IXF54_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1405407.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IXF54_002172"
     CDS             10539..11312
                     /locus_tag="IXF54_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405408.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IXF54_002173"
     CDS             11305..12096
                     /locus_tag="IXF54_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1405409.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IXF54_002174"
     CDS             12106..12597
                     /locus_tag="IXF54_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405410.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IXF54_002175"
     CDS             12615..13025
                     /locus_tag="IXF54_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1405411.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IXF54_002176"
     CDS             13029..14429
                     /locus_tag="IXF54_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1405412.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IXF54_002177"
     CDS             14452..14826
                     /locus_tag="IXF54_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1405413.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DACERL010000066        15284 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.070, whole genome shotgun sequence.
ACCESSION   DACERL010000066 DACERL010000000
VERSION     DACERL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.070"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(41..407)
                     /gene="ssrA"
                     /locus_tag="IXF54_002178"
     tmRNA           complement(41..407)
                     /gene="ssrA"
                     /locus_tag="IXF54_002178"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(578..1351)
                     /locus_tag="IXF54_002179"
     CDS             complement(578..1351)
                     /locus_tag="IXF54_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1405414.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1495..1845
                     /locus_tag="IXF54_002180"
     CDS             1495..1845
                     /locus_tag="IXF54_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1405415.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1826..2542
                     /locus_tag="IXF54_002181"
     CDS             1826..2542
                     /locus_tag="IXF54_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405416.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2542..3990
                     /locus_tag="IXF54_002182"
     CDS             2542..3990
                     /locus_tag="IXF54_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1405417.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4060..4569)
                     /locus_tag="IXF54_002183"
     CDS             complement(4060..4569)
                     /locus_tag="IXF54_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405418.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4559..5284)
                     /locus_tag="IXF54_002184"
     CDS             complement(4559..5284)
                     /locus_tag="IXF54_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405419.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5507..7627)
                     /locus_tag="IXF54_002185"
     CDS             complement(5507..7627)
                     /locus_tag="IXF54_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1405420.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7857..9035
                     /locus_tag="IXF54_002186"
     CDS             7857..9035
                     /locus_tag="IXF54_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1405421.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9051..9809
                     /gene="aroD"
                     /locus_tag="IXF54_002187"
     CDS             9051..9809
                     /gene="aroD"
                     /locus_tag="IXF54_002187"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1405422.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9832..10317
                     /gene="ybaK"
                     /locus_tag="IXF54_002188"
     CDS             9832..10317
                     /gene="ybaK"
                     /locus_tag="IXF54_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1405423.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10388..11641)
                     /locus_tag="IXF54_002189"
     CDS             complement(10388..11641)
                     /locus_tag="IXF54_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1405424.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11758..13107
                     /gene="gdhA"
                     /locus_tag="IXF54_002190"
     CDS             11758..13107
                     /gene="gdhA"
                     /locus_tag="IXF54_002190"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1405425.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13219..14565
                     /locus_tag="IXF54_002191"
     CDS             13219..14565
                     /locus_tag="IXF54_002191"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1405426.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14873..15259)
                     /locus_tag="IXF54_002192"
     CDS             complement(14873..15259)
                     /locus_tag="IXF54_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1405427.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4988 a         2737 c         3104 g         4455 t
ORIGIN      
        1 ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac ggcgggagtc
       61 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      121 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      181 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      241 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      301 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      361 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
      421 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
      481 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
      541 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
      601 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
      661 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
      721 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
      781 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
      841 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
      901 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
      961 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1021 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1081 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1141 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1201 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1261 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1321 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1381 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     1441 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     1501 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     1561 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     1621 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     1681 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     1741 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     1801 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     1861 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     1921 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     1981 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2041 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2101 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2161 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagtagt
     2221 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2281 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2341 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     2401 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     2461 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     2521 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttctgcgga
     2581 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     2641 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     2701 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     2761 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     2821 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     2881 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     2941 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3001 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3061 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3121 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3181 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3241 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3301 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3361 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     3421 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     3481 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     3541 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     3601 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     3661 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     3721 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     3781 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     3841 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     3901 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     3961 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4021 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4081 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4141 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4201 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4261 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4321 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4381 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     4441 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     4501 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     4561 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     4621 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     4681 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     4741 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     4801 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     4861 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     4921 taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc aattcctgct
     4981 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5041 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5101 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5161 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5221 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5281 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5341 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     5401 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     5461 gagcagcgac aataattttg tcgctgctcc ttttaaattt attttatcat tcggaagaac
     5521 ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt
     5581 gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt
     5641 ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac
     5701 tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt
     5761 tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa
     5821 tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa
     5881 caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc
     5941 ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat
     6001 caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca
     6061 tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc
     6121 caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa
     6181 ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta
     6241 cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat
     6301 tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata
     6361 cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt
     6421 ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat
     6481 aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga
     6541 atgtaccagc gttcccatgg aataccgcac taatgtaacc ttgtgtttga cttaaaatat
     6601 caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca
     6661 agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt
     6721 gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag
     6781 gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct
     6841 ggaagctttc taaatggata taaatcacat tttttcctct agcgagcccg aacaaatcgt
     6901 catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac
     6961 ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag
     7021 cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg
     7081 gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga
     7141 tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca
     7201 gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc
     7261 gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg
     7321 taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa
     7381 gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa
     7441 ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc
     7501 cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta
     7561 aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac
     7621 atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt
     7681 acacagctca cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc
     7741 agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag
     7801 acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga
     7861 aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa
     7921 aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc
     7981 aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat
     8041 tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa
     8101 aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg
     8161 gagaaggaga cgggatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt
     8221 cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa
     8281 tctacccgga aatcatagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg
     8341 agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag
     8401 taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag
     8461 aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta
     8521 gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg
     8581 tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg
     8641 atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa
     8701 gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga
     8761 aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt
     8821 tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat
     8881 tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt
     8941 tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga
     9001 aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggttcggg
     9061 taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg
     9121 tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg
     9181 aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg
     9241 ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg
     9301 aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac
     9361 acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa
     9421 gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta
     9481 aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag
     9541 ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg
     9601 agttgactag acgggcaaat gcttttttct ccatgcctat tattacgatg tccatgggaa
     9661 atcttggtaa aatttctcgt ctggttggcg gaacgacggg ttccgttatg acatttggtt
     9721 ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa
     9781 ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag
     9841 aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca
     9901 gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt
     9961 ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct
    10021 cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca
    10081 gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata
    10141 cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt
    10201 gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta
    10261 gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc
    10321 atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt
    10381 ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg
    10441 ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca
    10501 gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc
    10561 cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt
    10621 tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat
    10681 ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag
    10741 cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt
    10801 gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta
    10861 attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg
    10921 tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta
    10981 aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg
    11041 atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga
    11101 tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga
    11161 taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt
    11221 tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac
    11281 gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat
    11341 gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat
    11401 tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat
    11461 catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt
    11521 ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga
    11581 tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca
    11641 ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt
    11701 gagccaaaag agaaaatgag ctaaaatgag ggaagataag caaaggagcg tggaacgatg
    11761 acaaacgcaa cagaatatgt tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg
    11821 caagaagaat ttttacaagc aatcgatgaa ttcatgccca cggttatgtc ttttttggac
    11881 actcatcccg aatatatcga aaagaatatc ttagaacttt taacggaacc agaaagagtt
    11941 attcaatttc gagtgccttg gcaagatgat gaaggaaact ggcgagtaaa tagagggtac
    12001 cgtatccaat acaactcagc tatcggccct tataaaggtg gattgcgctt ccatcctagt
    12061 gtcaatctaa gtatcttgaa atttttggct tttgaacaga ttttcaagaa tagtttaacc
    12121 ggtctgccaa tcggtggagg aaaaggcgga agtgacttcg atcctaaagg aaaatctgat
    12181 aatgagatca tgcgtttttg tcaaagtttc atgctggaat tgtcaaaaca tatcggccca
    12241 tcgattgatg ttcctgcggg agatatcggt gtaggagcta gagaaatcgg ctatttgttc
    12301 ggcgaatata aacgattgaa aaaatatgat actggtgtgt tgacaggtaa acctttggat
    12361 ttttggggaa gtaagatacg tacggaagct actggttatg gattagtgta ttatgtcaaa
    12421 catctattga acgaagaaaa agattcattt aatcaaaaaa cagtttttgt ttccggaagc
    12481 gggaatgtag ccatttatgc aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca
    12541 tgttcagatt ctagcgggta catctatgat ccagaaggaa tagatgtgaa actattgaaa
    12601 gaagtcaaag aaatcaatag acaacgtttg acggaatacg caaaccaacg tccttctgct
    12661 atttatcatg ctggagaatc tgtttggaca ttgaaagaaa aagcggatat cgctttgcca
    12721 tgtgctactc aaaatgaaat agacgaaagc ttggccgaaa tcttagtaga aaatgggatc
    12781 aaaatcgtag cagaaggcgc taatatgcca tgtacagtaa aagctgtttc tgttcttcat
    12841 gaagcaggcg tgtggtattg tccaggaaaa gctgctaatg ctggaggagt agcagtatct
    12901 gctttagaaa tgagtcagaa cgtccagcga ttgacttggg aaagagagca agtggatgag
    12961 cagctggatc aaatcatgga aacgatttac catacgtgtc gccaaacagc agccgagtac
    13021 ggcgacgaaa aaaatctgct tcttggagca aatgttgctg gatttgaaaa agttgccaaa
    13081 gcaatggcag cagaaggttt agtttgaaaa atgaaacttt cttggaaata ttgtcagcat
    13141 tttgtttatt tgcttgaaaa ataataaaaa aaaacgtaaa gtattacctg tagagtaaac
    13201 ctataaggag gagaacaaat ggcacacatt cattttgact attcaaaagt agcaccgttt
    13261 gtcaacgaac aagagttagg gtacatgcaa agccaagtaa cagcagccca caacgaatta
    13321 cgggaaggta ctggcgcagg aagcgacttc cgcggctgga tcgatttacc aacgaactat
    13381 gacaaagaag aatttgcacg tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa
    13441 gtattagttg ttatcggtat cggtggttct tatttaggcg ctcgtgcagc gatcgacttc
    13501 ttgaatcaca cattcttcaa tttattagat agcaacacaa gaaaagcacc acaaatcttc
    13561 tttgccggaa attcaatcag ctcaacatat attgctgact tgatcgaagt aatcggtgat
    13621 cgcgactttt cagtaaacgt tatttcaaaa tctggaacaa ctactgaacc agcgattgcg
    13681 ttccgtgtat tcaaagagct tcttgttaag aaatatggac aagaagaagc aaacaaacgt
    13741 atctacgcta caacagataa agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt
    13801 tgggaaactt tcgtgattcc tgatgacatc ggcggacgtt tctctgtatt aactgctgtt
    13861 ggtttgttac caatcgctgt tagtggtgca gatatcgatg cattgatgca aggtgccgct
    13921 gatgcaagca aagcatactc aagcgacaaa ctagaagaaa acgaagctta ccaatacgca
    13981 gcaatgcgca acattcttta ccgtaaaggc aaagtaactg agttgttgat caactatgaa
    14041 ccaggaatgc aatacttctc agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa
    14101 gaccaaaaag gcatctatcc ttcaagcgca aacttctcaa ctgatttgca ctctttagga
    14161 caattcatcc aagaaggacg tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct
    14221 cgtaaatcaa tcacaattcc tgaacaagca gaagatttag acggtctagg ctacttgcaa
    14281 ggaaaagaag tggacttcgt aaatacaaaa gcatttgaag gaacattgct tgctcataca
    14341 gacggtgacg tgcctaactt gctagtcaaa attccagaaa tggatgctta tacattgggt
    14401 tatacaatgt acttctttga aattgctgta ggtatttcag gttacttgaa tggaatcaac
    14461 ccattcgacc aaccaggtgt agaagcttac aagaaaaaca tgttcgcact tctaggcaaa
    14521 ccaggctttg aagatcttgc taaagaattg aacgaacgcc tgtaagataa gggtttttcg
    14581 ctagatttaa ataatcagtg atagaaacat gttcgcttta aaatgaaaac tggtacccat
    14641 ttaggtactc aaacatactg catcgtttat tcagaataag gggctgtgac aagacgtttg
    14701 tcacagtccc ttgtttgtat agaaatcgat tgattgcctt gctctttagt ttgttttagg
    14761 actttaagaa cagtgaggct gacttcgaag gttaattctg tttttttgtt ttctagaatc
    14821 agtaagcaaa aaagcactca cttcgagtgc ttttttgcga tatattctta gcttaagata
    14881 ggcctaattt ttcttttaca gatgagggta ccttatcgct ggtaattact tttttctcta
    14941 tgatccgctt ttcgcttgct ttcaactgca agatcgttcc ttcggggtaa gcagaaccat
    15001 tggcagagtc accagaagta atgacatcga aatctaactg tcttttttca ccgttttcag
    15061 cataagcatc tacgttgtat ttaaaacctc ttccaattac ttccccatta tctgctttga
    15121 tatcagtttc tgcaggcatt ggagctttga ctaccgcata ataatcttta cctacataag
    15181 tagactgata gtagtcccaa cctttccatc ctataaaagc aatcaatatg ataacgatca
    15241 accctaaaat ttttttcatg aaagatctcc ttttttattt ttat
//
LOCUS       DACERL010000067        14995 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.071, whole genome shotgun sequence.
ACCESSION   DACERL010000067 DACERL010000000
VERSION     DACERL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.071"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXF54_002193"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXF54_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1405428.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IXF54_002194"
     CDS             complement(322..891)
                     /locus_tag="IXF54_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1405429.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IXF54_002195"
     CDS             1077..1979
                     /locus_tag="IXF54_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1405430.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXF54_002196"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXF54_002196"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1405431.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXF54_002197"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXF54_002197"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1405432.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IXF54_002198"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IXF54_002198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1405433.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IXF54_002199"
     CDS             8547..8732
                     /locus_tag="IXF54_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1405434.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IXF54_002200"
     CDS             complement(8794..9990)
                     /locus_tag="IXF54_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1405435.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IXF54_002201"
     CDS             complement(10160..11596)
                     /locus_tag="IXF54_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1405436.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IXF54_002202"
     CDS             11889..12698
                     /locus_tag="IXF54_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1405437.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="IXF54_002203"
     CDS             complement(12884..14881)
                     /locus_tag="IXF54_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1405438.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2661 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACERL010000068        14741 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.072, whole genome shotgun sequence.
ACCESSION   DACERL010000068 DACERL010000000
VERSION     DACERL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.072"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(547..909)
                     /locus_tag="IXF54_002204"
     CDS             complement(547..909)
                     /locus_tag="IXF54_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405439.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="IXF54_002205"
     CDS             complement(983..1363)
                     /locus_tag="IXF54_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1405440.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="IXF54_002206"
     CDS             1445..2620
                     /locus_tag="IXF54_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1405441.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2696..3280)
                     /locus_tag="IXF54_002207"
     CDS             complement(2696..3280)
                     /locus_tag="IXF54_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1405442.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3481..4479
                     /locus_tag="IXF54_002208"
     CDS             3481..4479
                     /locus_tag="IXF54_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1405443.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4591..5100
                     /locus_tag="IXF54_002209"
     CDS             4591..5100
                     /locus_tag="IXF54_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1405444.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5130..5789)
                     /locus_tag="IXF54_002210"
     CDS             complement(5130..5789)
                     /locus_tag="IXF54_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1405445.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5791..6555)
                     /locus_tag="IXF54_002211"
     CDS             complement(5791..6555)
                     /locus_tag="IXF54_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1405446.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6563..7240)
                     /locus_tag="IXF54_002212"
     CDS             complement(6563..7240)
                     /locus_tag="IXF54_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1405447.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7277..8662)
                     /locus_tag="IXF54_002213"
     CDS             complement(7277..8662)
                     /locus_tag="IXF54_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1405448.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8872..8956)
                     /gene="ffs"
                     /locus_tag="IXF54_002214"
     ncRNA           complement(8872..8956)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXF54_002214"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IXF54_002215"
     CDS             complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IXF54_002215"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1405449.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9601..10458
                     /locus_tag="IXF54_002216"
     CDS             9601..10458
                     /locus_tag="IXF54_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1405450.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10539..10874)
                     /locus_tag="IXF54_002217"
     CDS             complement(10539..10874)
                     /locus_tag="IXF54_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405451.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10941..12311)
                     /locus_tag="IXF54_002218"
     CDS             complement(10941..12311)
                     /locus_tag="IXF54_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1405452.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12389..12925)
                     /locus_tag="IXF54_002219"
     CDS             complement(12389..12925)
                     /locus_tag="IXF54_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1405453.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13234..14715
                     /locus_tag="IXF54_002220"
     CDS             13234..14715
                     /locus_tag="IXF54_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1405454.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4527 a         2925 c         2394 g         4895 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag
     2641 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2701 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2761 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2821 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2881 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2941 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     3001 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     3061 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     3121 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     3181 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     3241 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     3301 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     3361 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     3421 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3481 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3541 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3601 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3661 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3721 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3781 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3841 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3901 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3961 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     4021 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     4081 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     4141 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     4201 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     4261 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     4321 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     4381 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     4441 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4501 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4561 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4621 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4681 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4741 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4801 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4861 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4921 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4981 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     5041 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     5101 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     5161 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     5221 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     5281 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     5341 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     5401 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     5461 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5521 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5581 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5641 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5701 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5761 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5821 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5881 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5941 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     6001 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     6061 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     6121 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     6181 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     6241 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     6301 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     6361 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     6421 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6481 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6541 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6601 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6661 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6721 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6781 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6841 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6901 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6961 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     7021 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     7081 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     7141 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     7201 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     7261 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     7321 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     7381 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     7441 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7501 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7561 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7621 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7681 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7741 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7801 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7861 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7921 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7981 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     8041 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     8101 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     8161 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     8221 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     8281 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     8341 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     8401 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     8461 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8521 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8581 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8641 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8701 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8761 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8821 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8881 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8941 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     9001 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     9061 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     9121 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     9181 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     9241 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     9301 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     9361 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     9421 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9481 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9541 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9601 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9661 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9721 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9781 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9841 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9901 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9961 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat caacggtatt
    10021 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
    10081 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
    10141 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
    10201 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
    10261 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
    10321 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
    10381 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
    10441 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10501 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10561 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10621 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10681 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10741 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10801 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10861 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10921 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10981 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    11041 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    11101 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    11161 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    11221 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    11281 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    11341 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    11401 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    11461 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11521 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11581 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11641 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11701 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11761 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11821 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11881 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11941 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    12001 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    12061 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    12121 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    12181 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    12241 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    12301 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    12361 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    12421 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12481 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12541 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12601 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12661 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12721 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12781 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12841 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12901 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12961 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    13021 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    13081 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    13141 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    13201 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    13261 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    13321 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    13381 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    13441 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13501 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13561 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13621 aaacagctca atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc
    13681 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13741 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13801 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13861 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13921 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13981 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    14041 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    14101 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa
    14161 cagtttcctc tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg
    14221 gcagttgttt ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt
    14281 ccagaagcca cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa
    14341 atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa
    14401 ttgatccaac acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata
    14461 accaaacagt ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa
    14521 gatgctgtta aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat
    14581 gagaaaattt cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta
    14641 tttggcaagt taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa
    14701 acaaagagga tgtgacaaaa ttcttgtcac atcctcttat t
//
LOCUS       DACERL010000069        14548 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.073, whole genome shotgun sequence.
ACCESSION   DACERL010000069 DACERL010000000
VERSION     DACERL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.073"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            4..816
                     /locus_tag="IXF54_002221"
     CDS             4..816
                     /locus_tag="IXF54_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1405455.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IXF54_002222"
     CDS             813..1613
                     /locus_tag="IXF54_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1405456.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IXF54_002223"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IXF54_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1405457.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IXF54_002224"
     CDS             3110..4819
                     /locus_tag="IXF54_002224"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1405458.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IXF54_002225"
     CDS             5031..9389
                     /locus_tag="IXF54_002225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1405459.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IXF54_002226"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IXF54_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1405460.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IXF54_002227"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IXF54_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1405461.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IXF54_002228"
     CDS             11227..11520
                     /locus_tag="IXF54_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1405462.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IXF54_002229"
     CDS             11517..11828
                     /locus_tag="IXF54_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1405463.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IXF54_002230"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IXF54_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1405464.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IXF54_002231"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IXF54_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1405465.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3768 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaa
//
LOCUS       DACERL010000070        14121 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.074, whole genome shotgun sequence.
ACCESSION   DACERL010000070 DACERL010000000
VERSION     DACERL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.074"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(119..775)
                     /locus_tag="IXF54_002232"
     CDS             complement(119..775)
                     /locus_tag="IXF54_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1405466.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            968..1246
                     /locus_tag="IXF54_002233"
     CDS             968..1246
                     /locus_tag="IXF54_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405467.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1265..2473
                     /locus_tag="IXF54_002234"
     CDS             1265..2473
                     /locus_tag="IXF54_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1405468.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2621..2986)
                     /locus_tag="IXF54_002235"
     CDS             complement(2621..2986)
                     /locus_tag="IXF54_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1405469.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3133..3906)
                     /locus_tag="IXF54_002236"
     CDS             complement(3133..3906)
                     /locus_tag="IXF54_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1405470.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4080..4940)
                     /locus_tag="IXF54_002237"
     CDS             complement(4080..4940)
                     /locus_tag="IXF54_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1405471.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4940..6358)
                     /locus_tag="IXF54_002238"
     CDS             complement(4940..6358)
                     /locus_tag="IXF54_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1405472.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6415..6864)
                     /locus_tag="IXF54_002239"
     CDS             complement(6415..6864)
                     /locus_tag="IXF54_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1405473.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7309..7572)
                     /locus_tag="IXF54_002240"
     CDS             complement(7309..7572)
                     /locus_tag="IXF54_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1405474.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7671..8966)
                     /locus_tag="IXF54_002241"
     CDS             complement(7671..8966)
                     /locus_tag="IXF54_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1405475.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8963..10234)
                     /locus_tag="IXF54_002242"
     CDS             complement(8963..10234)
                     /locus_tag="IXF54_002242"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1405476.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10399..11268)
                     /locus_tag="IXF54_002243"
     CDS             complement(10399..11268)
                     /locus_tag="IXF54_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1405477.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11560..13170)
                     /locus_tag="IXF54_002244"
     CDS             complement(11560..13170)
                     /locus_tag="IXF54_002244"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1405478.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         4029 a         2972 c         2403 g         4717 t
ORIGIN      
        1 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
       61 ttccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttgttcatc
      121 aaaagataat attgtcttct aacaagtcca tattcaaaat aacgatatga tggtctttaa
      181 cggtgataat accatctttt ttcagttgtt ttaaaattct gtttacactg ctagcagttg
      241 agatgccaca aaatttggcg atttcttcat tcgtgatcga aaaatcgatc agcatccctt
      301 cttctgtccg cacaccgaat aaatcatata attcgtaaag ttgcgtacaa acagctccca
      361 gctttccgtt taatagcatt tgctgcattt ttttcatcga ataaagcaga cgatagcggt
      421 aatactcttt tacatagtgt tgtagctttg ggtcttggtt gatattttgc caaaagcgca
      481 cacgattgat tcgataaaac tctgcatatt ctgattcaac tcgaatatta tacggcgaat
      541 cgatgaattg tgaatattcg tcttttaaaa gagagacgat ttcaagatct ttgatatagc
      601 gaaggttaaa ttctctccca tcatttgaaa taacacttgc ttttaccgtt ccctctttca
      661 agatatacgt atgggaatct tccatccctt cgaatgtcaa atatttatga tatcctttgc
      721 gtataatcgg aaaatcattt ttatccaaat attcctttaa gactctacta tccaatgatg
      781 ctcccccatc caaactttca tctaaaaaga tcatacagta ctttaaaact ttttaaagtc
      841 caaaggtatt ttactaatga tattaacatt tgttaagtac ctttttccat cacttatgcg
      901 atttttttcc ttttttttat tcgttatact gcagaagtaa aattttttaa gaatagaggg
      961 aaaaaacatg aattactgga tgaacacaat cattaatcga ttagaaacag catatcaaac
     1021 aagatttgat atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa actcgatcga
     1081 attgatcaaa gcagtggatg agaatccaac aaacgaatgc gaagaatttt taaatctgtt
     1141 tatgtctaca agagatttat ttattcgcca acttgttgat cgctatccat ccaattatca
     1201 cgatgtagaa gttcaaatac aaaaattaaa agcatattca gcttaagggg aatgtataac
     1261 atacatggaa aagaaattat ttaataaata ttttattgga atcacattga tcaattttat
     1321 tgtctacctg atttattatc tgttgatggt catcatggca gtcattgcgc aagacacctt
     1381 acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg gaacattgct
     1441 ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta ccttaagagg
     1501 cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa ctatcggggc
     1561 attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta cagctacaaa
     1621 tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca attattatgg
     1681 attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt tgctgaatat
     1741 gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg ttacgattgc
     1801 ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa tatctcttcg
     1861 ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag gaattgcctt
     1921 tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata caaaagtaat
     1981 ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca tcacatttac
     2041 acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga tgtatcctag
     2101 ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata gtgtgacatt
     2161 gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa atggacaggc
     2221 tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta cctactttat
     2281 tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa aatctcttac
     2341 ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta cagcactcta
     2401 tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg aatcattgaa
     2461 aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga aaataatctg
     2521 agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct atttattttt
     2581 aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt ttccgttcat
     2641 ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc acaccgatca
     2701 atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata aaatatagat
     2761 agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg atgttcccta
     2821 ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata attaccccaa
     2881 caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc cacagaatcg
     2941 cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc tgcactcctt
     3001 tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat tataccatcg
     3061 gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca cttagaacat
     3121 tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt tcgcatactg
     3181 ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag caatggcttg
     3241 atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc ggtctgattg
     3301 ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta ctccttggct
     3361 gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca atgcatcgtt
     3421 ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc ccaatacaga
     3481 tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat tcgtaatgat
     3541 ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg tactggaatc
     3601 caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa tcttacgttt
     3661 ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa tttttcgatt
     3721 gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt ccagaaaaac
     3781 aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat cggtgactct
     3841 tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct ccacttcatt
     3901 caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt tgtgacagaa
     3961 gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg aacatcgtgt
     4021 gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca tttttatttt
     4081 tagtataaac tagccttgcc tactgtgttc aaaatttcca ttttatgttt gaccacttct
     4141 tgtacctttt tattagctgc tggataaatt gcattcggct cgtacatcga tggattttca
     4201 ctcaatattt ctctcaaagc attgaaaaag acgcttttaa gatctgagct caaattcact
     4261 ttggcaactc cgtattttac tgcttcgctg acttctgcgt ccggattacc tgaaccgcca
     4321 tgtaaaacaa atggaagatc gatttgttgg ttcaattctt tcagtaagtc gatatttaat
     4381 tttggagttc tgtttttcgg atacaagcca tgcgctgttc caattgctac agcaagtgta
     4441 tcgattcctg tttctttcac aaaaatcttc gcttgatcag gatctgtata aatgattgtt
     4501 tcagcacctc cttcagcaga accattattc ccaatcgttc caagctcagc ctccactgag
     4561 acatttaccg catgagctaa cgcaaccact tttttcgtaa tctgtacatt gtcgtcgaat
     4621 gatttcaaag acgcatcgat catcacggaa gaatagccgt ttcgaatcgc ccgtaagaca
     4681 tcttctatcg taccgccatg atccaagtgg ataacgatcg gcagtttact tgtataagca
     4741 taggctctta ctgccgcaac aaactcatca cttaaatagg cgatttcatc aggatgaatt
     4801 tctaagatta ctggtgcttc taatctctct gcttcttcga tgattgcttt catcatatca
     4861 tagctgcaaa tattgaaggc tggtactgca aattgattct ctttagccac tttcaataac
     4921 tctttcattg tatataacat tacgcttcca cctcatttgt tagattttca tttttcgtcc
     4981 acttgtcttg ttttttctct ttcactgttt ttttcaatag tccaagcata aaggctgtag
     5041 tgactgtacc tgatgcaatt gccaagacat acataggtaa attgttcata acatttggaa
     5101 ttagcatcac ccaaatccct ccatgaggtg ccattgaagt aactcctact gccatggaaa
     5161 tccctgcagc gactgctgaa cctgtcatca aactaggtat cacgcggatc gggtcagata
     5221 ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc taaaatccag catgattgac
     5281 ctgaagtccg ttcttctttg gtaaatttat gtttgaataa taaagtggct aaagcaatac
     5341 ctaggggagg tgtcattcct gcagccatac aagcagcaat cggtgcattt actccagctg
     5401 acatcaaacc gatcgagaaa gtcgaagtcg atttattgat cggcccaccc atatcagctg
     5461 ccatcatgat cccgataagt aatccgaaca atagcccgga tgtatttccc aagctattca
     5521 gccaatttgt caaagcatcc aacacccatg tgatcggttg ttcaatcaca aataccatca
     5581 aaagcccaat aattaagatc gataaaaagg ggacgacaat cagctgataa atagcagaaa
     5641 attctttttt tacgctaatt ttatttgcca gaaaatcaca gatataccct gctagcacgc
     5701 caccaatcat tccgccaaga aaacctgaac cacctgtaga ggccagtaat ccggcaacaa
     5761 ttcctggggc aaaacctgct tttccagcaa tcgatgtagc aatccccgcg cctaacgcag
     5821 gaaccatcat ggcaaacgct gtatctcctc cgatccttga aaatgcagct gctaacacat
     5881 tatagtctgc tgagttggga tcagccgctg taatcccaaa ggcaaaactc agtgcgatga
     5941 tgattccgcc tgcgatcaca aatggaagca tgtaattcac accattcatg aaatgattat
     6001 agattgtcgg ctttttctca tttgcagctg gttctttttc attagacaca acgaatacgc
     6061 caatatctct caatgcttcg tctattacta cttgcggttc tttgattgct cggcttgtag
     6121 agacacgctt tacttttttc ccggcaaatc ggtccaattc gatttcttta tctactgcaa
     6181 gaatgattgc atctgcctct ttgatttctt gttcagataa ctgattttct gtttttacac
     6241 cttgtgtttc gaattttgct tgatggttta gatttcttgc tgttacttct aatttttctg
     6301 ccgacatata agtatgagca attccagtcg ggcaagcagt tacaccaacg attttcatga
     6361 ttattttcct cctttttctt cactatcgat actcggacaa agactctact attttcattc
     6421 ttgactaatc tgtctgatga tatctgctgg cgtagtggct tcttttattc cctttctaaa
     6481 atctgcattc atcaatcgtc tggagagtgt gctgagaatc tctagatgca tatctgctga
     6541 tttttcagga actacgatgg caaaaatata ttcaattggc atttcatcta tcccgctata
     6601 gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat atttgctttt
     6661 tgcatgggga atcccaaatc catcttctat tcctgtggag atctcctcct ctcgtttgaa
     6721 tagtgcctta acgacttttt gaacatttct taccttattt gtttttttca acacatcccc
     6781 tattcgctca aataatgatc cttttgaatc tatcgatgga tcgaaaacaa tcagttcttc
     6841 tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg ttaacgtttt
     6901 cttggaactg aggtcagtat agcatcgcta gctgtaccgt tcaatattat gaatgaacag
     6961 aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct agctcacaaa
     7021 caatcgcaag tctataacaa aacaaaaagc cttccttgaa aggaaagctt agcttctaat
     7081 tttaggtaaa tggtttttta cttcgaaata acccctcttc atttcgttaa tataaggtgt
     7141 ttttatcaag tggataatta aaactatata ctagtttttc attgttttgt gcattatgat
     7201 cctacgactt tttcagcatc aaacgaagtt ttaaattggc aaaaaacata aaaaaacaga
     7261 ctatccctat cagatagtct gctaaacgtt gagttatcag cgtttttatt attccgcagc
     7321 gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc acacgtccgt ctgcttgtgt
     7381 gaatttttga cgtccagtgt agaatggatg tgagtcagaa gttacttcga cacggatgac
     7441 tgggtatgta ttaccgtctt cccattcaac tgtttcttgt gaagtttttg ttgaacctga
     7501 caagaatttg aaaccagttg ttgaatccat gaatactact gggtggtaat ctggatggat
     7561 gttttctttc atgatttttt cgctcctttg ccctgctcca tttgctgaac cagagttgat
     7621 ttgtcgattt acaacattaa catcttaaca tgtttcagtc aaggttgcaa ttatcttttc
     7681 agatttctgg tcggattttt tttaccaaag gagacatctt gaaattcttt gaaaaattgc
     7741 tgattgtttt ttgttttacg caaaaagcgg atcagttgtt ccgtatattc taatgaatct
     7801 ccagtcatat tttttcgaag tttccaaatt tcttctaatt cttcacttgg catcaataat
     7861 tcttcttttc gggtacctga ttttttgata tcgatggctg ggaagatccg acgttcagat
     7921 aattcacgtg aaagttgtaa ttctagattt ccagttcctt tgaattcttc atagatcacg
     7981 tcatccatac ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa actgccacct
     8041 tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc ggctgggtca
     8101 atcccgccac tcaatgtacg tccgcttggt ggaacgacta agttataggc ccgagcaagt
     8161 ctagtaatac tatccatcaa gataacgaca tctcgtttat cttcgactaa acgcattgcg
     8221 cgttccaata ctaattcaga aactcgagta tggttttgcg gctgcagatc aaatgtagaa
     8281 gataccacgt cgcctttcac gcttcgttct agatccgtta cttcttccgg tcgttcatca
     8341 atcagtagaa cgattaattc tacttccgga tagttttcgg tgataccatt tgcgatttct
     8401 tttaatacag aagttttccc tgcttttggc ggtgcgacga tcaatccacg ctgaccaaaa
     8461 ccgattggag aaaagatatc gatcattcgg gtagatagtc tttgcggtgt tgtttccagt
     8521 tgaagctgtc tttgcggata aagaggtgtt aaggctggaa aatgcggacg ttcttttgct
     8581 tcttccggat cttttccatt aacgctttcc acgtgcatca aaccataata acgttccgat
     8641 tctttaggtg gacgggcttt tcctgccact ttgtccccat ttctcaaact gaatcgtctg
     8701 atttgtgagg cagaaatata gatatcttct gcacttggtc cataattgat tggccgtaaa
     8761 aaaccgtaac catcttgacc aacgatatcc aatattcctt ccatataata aaatccttgt
     8821 ttctctgctt gtgcacgaat cacagctaga gataattcct ttttattcat cttactgtaa
     8881 taaggaattt taaattcttt ggcataacta taaacttctt ttaacgtttt gttctctagt
     8941 tctgccattg tcaaataatc gctcattcag ctcccacttc ctctatcatt tcaattgatg
     9001 cccctaaggc tgttagtttc tcgataatat gatcataacc gcgtaagata taatctacat
     9061 tgtaaatcgt cgttgtccct tctgccaata gtccagcaat cactaaacaa gcacctgccc
     9121 gaagatcaga agccacgact tctgtgccgt gaagtttatt tggaccattc acgatgatca
     9181 tatttccttc cacggtaata tcagcgccca ttcgtgctag ttccggcaca tggttcacac
     9241 gtttcgcata gatcgtatcg atgatttctg aagtgcctgt agtcatcaat agtaacgcag
     9301 taagtggctg ctgcagatct gtggcaaatc ctggataagg ataggtcatg atgtttgcgg
     9361 gttttaattc tttcgagggg taaacttcga tttcatcttc gctgattttc atatggacac
     9421 caatttcctg caattttgct ataaagcttt ccaaatgttc aaagatcaca tttttcactt
     9481 tgataccatt tcctactgca gctgctaatg ctagataggt tcctgcttcg atgcggtcag
     9541 gaatcatgaa atgccgacag ccatgtaatg tttcgactcc ttcgatacgg atcacatctg
     9601 tacccgctcc tcgaactttt gcccccatat tattcagtaa agttgccaca tctatgattt
     9661 ccggttctcg tgcagcgttt tcaattactg tctgtccttt tgcttttaca gcagctaaca
     9721 tcacattgat cgttgctccg actgaaacca cgtccatgaa aatccgattg ccacgcaatc
     9781 ctttattttc tgtccgtaaa tacatggcac catgttcatt tgtcacttca gcacctagag
     9841 cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc taaataacag ccacctggca
     9901 ggcctactac cgcttcgcca aatttaccaa gcagtgttcc cataaaataa taagaagcac
     9961 gcaaactatt gatttttccg tgcggcatcg gaatcgatac gacttctcga ggatcgattt
    10021 ctagtatgtt gttggaaaaa tggacagttg cgcccatgat ctctaaaatt tcaattaatg
    10081 aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac tggggaatca gctaagattg
    10141 cagctggaat caatgcgact gcactatttt ttgcaccgct gatcgttact tcaccagcta
    10201 aaggtcggtt tcctttgatt acgatttttt tcatctgttt ccaccttgtt tattaatctt
    10261 ttttcatgga tatgcttgta ttttatcata gataaaaaaa agaaacgagc atggtctgcc
    10321 gttttaagta aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg
    10381 tatctgttat gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt
    10441 agcatcttca acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt
    10501 accttcttta tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg
    10561 gaattcagtg ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc
    10621 ttgaggaata cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc
    10681 aattgcttgt aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat
    10741 gttaccgata ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc
    10801 aatatcagct aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac
    10861 ttcacattca actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt
    10921 caagttttct tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc
    10981 gatacattcc aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt
    11041 gatgttcatt gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat
    11101 gtatttagct gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc
    11161 ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc
    11221 gcctttacga gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct
    11281 cctaagtttt gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc
    11341 aaacatttcg gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt
    11401 gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt
    11461 tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc
    11521 cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc
    11581 taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa
    11641 ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac
    11701 taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg
    11761 atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc
    11821 atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa
    11881 agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc
    11941 tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa
    12001 ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg
    12061 agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac
    12121 caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc
    12181 tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc
    12241 aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac
    12301 ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc
    12361 tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac
    12421 taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac
    12481 atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac
    12541 caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg
    12601 tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat
    12661 aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga
    12721 ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt
    12781 agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt
    12841 tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt
    12901 cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc
    12961 caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat
    13021 cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg
    13081 gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac
    13141 accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg
    13201 caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga
    13261 aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact
    13321 aagggaaagt caagtactca aatgaatact atccgcttac aattttttct ttttcgatat
    13381 cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat
    13441 aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa
    13501 aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca
    13561 cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa
    13621 caatcttttt ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct
    13681 cggatttttg gcatatcttt tctattaaat aatatcaaaa acaaaacaat tccactaatt
    13741 ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg
    13801 aatcacataa gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta tttctaaata
    13861 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccatcaa acatgagaag
    13921 ctgtttttga aattatttct tgtgattcgg agttaaatgg taggctcaca acctctggat
    13981 aaacggtatt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa
    14041 gccattttca caaaattttt cttaatactc gaaactaaac gtctttttca aaacctcttg
    14101 gtaaacggtg ttcaaaagct t
//
LOCUS       DACERL010000071        14062 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.075, whole genome shotgun sequence.
ACCESSION   DACERL010000071 DACERL010000000
VERSION     DACERL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.075"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXF54_002245"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXF54_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1405479.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXF54_002246"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXF54_002246"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1405480.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IXF54_002247"
     CDS             complement(2169..2906)
                     /locus_tag="IXF54_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1405481.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IXF54_002248"
     CDS             complement(2997..3524)
                     /locus_tag="IXF54_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1405482.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IXF54_002249"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IXF54_002249"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1405483.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IXF54_002250"
     CDS             complement(4401..4886)
                     /locus_tag="IXF54_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1405484.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IXF54_002251"
     CDS             complement(5039..5236)
                     /locus_tag="IXF54_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405485.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXF54_002252"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXF54_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1405486.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXF54_002253"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXF54_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1405487.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IXF54_002254"
     CDS             9211..9423
                     /locus_tag="IXF54_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1405488.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IXF54_002255"
     CDS             9425..11104
                     /locus_tag="IXF54_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1405489.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IXF54_002256"
     CDS             complement(11358..11558)
                     /locus_tag="IXF54_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1405490.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IXF54_002257"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IXF54_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IXF54_002258"
     tRNA            complement(12249..12322)
                     /locus_tag="IXF54_002258"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IXF54_002259"
     CDS             complement(12634..13032)
                     /locus_tag="IXF54_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405491.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IXF54_002260"
     CDS             complement(13025..13477)
                     /locus_tag="IXF54_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405492.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IXF54_002261"
     CDS             complement(13464..13871)
                     /locus_tag="IXF54_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405493.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
BASE COUNT         4152 a         2947 c         2365 g         4598 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcaacggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catataccat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    14041 tgtatacttt ttgtttgata tc
//
LOCUS       DACERL010000072        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.076, whole genome shotgun sequence.
ACCESSION   DACERL010000072 DACERL010000000
VERSION     DACERL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.076"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXF54_002262"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXF54_002262"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1405494.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXF54_002263"
     CDS             complement(2260..2511)
                     /locus_tag="IXF54_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1405495.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXF54_002264"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXF54_002264"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1405496.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IXF54_002265"
     CDS             3461..3679
                     /locus_tag="IXF54_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405497.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IXF54_002266"
     CDS             complement(3968..4480)
                     /locus_tag="IXF54_002266"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1405498.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXF54_002267"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXF54_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1405499.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IXF54_002268"
     CDS             complement(6933..7367)
                     /locus_tag="IXF54_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1405500.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IXF54_002269"
     CDS             complement(7377..8165)
                     /locus_tag="IXF54_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1405501.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IXF54_002270"
     CDS             complement(8162..9103)
                     /locus_tag="IXF54_002270"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1405502.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IXF54_002271"
     CDS             complement(9280..9867)
                     /locus_tag="IXF54_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1405503.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXF54_002272"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXF54_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1405504.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IXF54_002273"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IXF54_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1405505.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IXF54_002274"
     CDS             complement(12784..13719)
                     /locus_tag="IXF54_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1405506.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACERL010000073        13794 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.077, whole genome shotgun sequence.
ACCESSION   DACERL010000073 DACERL010000000
VERSION     DACERL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.077"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            140..460
                     /locus_tag="IXF54_002275"
     CDS             140..460
                     /locus_tag="IXF54_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405507.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IXF54_002276"
     CDS             912..1112
                     /locus_tag="IXF54_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1405508.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IXF54_002277"
     CDS             1259..4048
                     /locus_tag="IXF54_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1405509.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IXF54_002278"
     CDS             4095..4622
                     /locus_tag="IXF54_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1405510.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IXF54_002279"
     CDS             4643..5833
                     /locus_tag="IXF54_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1405511.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IXF54_002280"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IXF54_002280"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1405512.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="IXF54_002281"
     CDS             7966..8640
                     /locus_tag="IXF54_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1405513.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="IXF54_002282"
     CDS             8637..8978
                     /locus_tag="IXF54_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405514.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="IXF54_002283"
     CDS             9008..9367
                     /locus_tag="IXF54_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1405515.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9561..9749
                     /locus_tag="IXF54_002284"
                     /pseudo
     CDS             9561..9749
                     /locus_tag="IXF54_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="IXF54_002285"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="IXF54_002285"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1405516.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="IXF54_002286"
     CDS             12286..13752
                     /locus_tag="IXF54_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1405517.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4579 a         2392 c         2938 g         3885 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct actt
//
LOCUS       DACERL010000074        13691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.078, whole genome shotgun sequence.
ACCESSION   DACERL010000074 DACERL010000000
VERSION     DACERL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.078"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(221..1177)
                     /locus_tag="IXF54_002287"
     CDS             complement(221..1177)
                     /locus_tag="IXF54_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1405518.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IXF54_002288"
     CDS             1281..1883
                     /locus_tag="IXF54_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1405519.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IXF54_002289"
     CDS             complement(1971..3470)
                     /locus_tag="IXF54_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1405520.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IXF54_002290"
     CDS             complement(3486..4010)
                     /locus_tag="IXF54_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405521.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IXF54_002291"
     CDS             4308..5057
                     /locus_tag="IXF54_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1405522.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IXF54_002292"
     CDS             5048..5833
                     /locus_tag="IXF54_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1405523.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IXF54_002293"
     CDS             5802..6518
                     /locus_tag="IXF54_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1405524.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IXF54_002294"
     CDS             complement(6620..7639)
                     /locus_tag="IXF54_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1405525.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IXF54_002295"
     CDS             complement(7658..8716)
                     /locus_tag="IXF54_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1405526.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IXF54_002296"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IXF54_002296"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1405527.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IXF54_002297"
     CDS             complement(10161..10997)
                     /locus_tag="IXF54_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405528.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IXF54_002298"
     CDS             complement(11181..12398)
                     /locus_tag="IXF54_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1405529.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IXF54_002299"
     tRNA            12781..12864
                     /locus_tag="IXF54_002299"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..>13691)
                     /locus_tag="IXF54_002300"
     CDS             complement(13104..>13691)
                     /locus_tag="IXF54_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405530.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4138 a         2794 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa a
//
LOCUS       DACERL010000075        13482 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.079, whole genome shotgun sequence.
ACCESSION   DACERL010000075 DACERL010000000
VERSION     DACERL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.079"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(164..388)
                     /locus_tag="IXF54_002301"
     CDS             complement(164..388)
                     /locus_tag="IXF54_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1405531.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IXF54_002302"
     CDS             complement(586..2166)
                     /locus_tag="IXF54_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1405532.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IXF54_002303"
     CDS             complement(2567..3943)
                     /locus_tag="IXF54_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1405533.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IXF54_002304"
     CDS             complement(4071..5189)
                     /locus_tag="IXF54_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1405534.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IXF54_002305"
     CDS             5331..5765
                     /locus_tag="IXF54_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405535.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IXF54_002306"
     CDS             complement(5843..6376)
                     /locus_tag="IXF54_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1405536.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IXF54_002307"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IXF54_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1405537.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IXF54_002308"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IXF54_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1405538.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IXF54_002309"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IXF54_002309"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1405539.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IXF54_002310"
     CDS             complement(10101..10679)
                     /locus_tag="IXF54_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1405540.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="IXF54_002311"
     CDS             11001..11471
                     /locus_tag="IXF54_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1405541.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="IXF54_002312"
     CDS             complement(11579..12805)
                     /locus_tag="IXF54_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1405542.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3954 a         2718 c         2248 g         4562 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gc
//
LOCUS       DACERL010000076        12669 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.081, whole genome shotgun sequence.
ACCESSION   DACERL010000076 DACERL010000000
VERSION     DACERL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.081"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            5..304
                     /locus_tag="IXF54_002313"
     CDS             5..304
                     /locus_tag="IXF54_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405543.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="IXF54_002314"
     CDS             377..1738
                     /locus_tag="IXF54_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1405544.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="IXF54_002315"
     CDS             1760..2020
                     /locus_tag="IXF54_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405545.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="IXF54_002316"
     CDS             2034..2786
                     /locus_tag="IXF54_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1405546.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="IXF54_002317"
     CDS             complement(2880..3485)
                     /locus_tag="IXF54_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1405547.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="IXF54_002318"
     CDS             complement(3538..4512)
                     /locus_tag="IXF54_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1405548.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5669)
                     /locus_tag="IXF54_002319"
     CDS             complement(4890..5669)
                     /locus_tag="IXF54_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1405549.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="IXF54_002320"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="IXF54_002320"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1405550.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="IXF54_002321"
     CDS             7160..8557
                     /locus_tag="IXF54_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1405551.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="IXF54_002322"
     CDS             8638..9078
                     /locus_tag="IXF54_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1405552.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="IXF54_002323"
     CDS             9226..9552
                     /locus_tag="IXF54_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1405553.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="IXF54_002324"
     CDS             9553..10302
                     /locus_tag="IXF54_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405554.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="IXF54_002325"
     CDS             complement(10382..10837)
                     /locus_tag="IXF54_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1405555.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11264..11539)
                     /locus_tag="IXF54_002326"
     CDS             complement(11264..11539)
                     /locus_tag="IXF54_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405556.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11833..12648
                     /gene="thiD"
                     /locus_tag="IXF54_002327"
     CDS             11833..12648
                     /gene="thiD"
                     /locus_tag="IXF54_002327"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1405557.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaaac
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta
    11221 ttttattaca gaacattttt aattaataaa gaacagttag aagttaaaat tgctgattca
    11281 catagagttt atgacttctt ggaaattctt tttcaatcag ttttttcatc tgtttatgaa
    11341 attctttttt ttcagtaaac ataaaggttt gggtatcgta accttcttca tgtaggaaat
    11401 agattattcc tttttctgtg ttttgcgtat aagcttttat actcctgtct tttttgaaag
    11461 aacagatcgt atagccatct atttggtctg gtagtcgttt gattttcttc aaaatgactt
    11521 ttttatcctc aagtttcatt tttttcacct gactttattt ttttcatatg gaaatcatag
    11581 cgcctttggt tatatgaaaa caagaaggat actgaaatat atataaatca aatagtggtt
    11641 atttattatt agaatagcga atacaaataa acgaccacta ttttctgatt tattgcaaat
    11701 gttttttcaa ataaatttga ttaccaagaa aaggtagtgg tatatttact gagggatact
    11761 ttgaaaaaca tatgatagaa acagtaacag tattatgaaa aatcttttta taaaaatgga
    11821 ggaatcgatt tgatggaaga tcagtatgta caagcaatga cgatcgcagg atcagatagc
    11881 gatggaagcg cagggatgca agcagatatg catacattct ttacaagaaa tgtttacggt
    11941 gtttctgtca tgaccgcatg tgttgcaggt aattcttacg gaatcggagc aagtgtgaca
    12001 ttgccaacag attttatcga caaagaattt gaactgattg cgaaggactt tcaaatacgt
    12061 gcagctaaaa caggtatgct agcagattca aagttgattg agacagttgt aaaaaattac
    12121 caaaaatatg attttggacc attagtcgtg gatcccgtaa tcgtaacgaa acatggaaat
    12181 cttttactag aagaatctgc tttgcaaagc ttgaaagaaa agcttgtccc tttggccgaa
    12241 gtactgacac ctaattttta tgaagcagaa aagctgactg gacggacatt agcagctgaa
    12301 gaagattttg tgcaagcagc aaaagaactg caagatatgg gtgcaaaaaa cgttatgatc
    12361 aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca atgactacgt attgttcgaa
    12421 tcaggaaaat cattttggtt aaatgcacca ttttatccta ccgaccggat caacggaact
    12481 ggcgattcgc tatctgcatg tattgcagct gagttagcga aaggaaaaac agttgaacaa
    12541 gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc gtgaagaaat aaatgtaggg
    12601 cacaaatatg gccccatcaa tcattgggca gctggtgaat tagagtaaaa gaaagaagtt
    12661 gtgaaaaag
//
LOCUS       DACERL010000077        12157 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.082, whole genome shotgun sequence.
ACCESSION   DACERL010000077 DACERL010000000
VERSION     DACERL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.082"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            578..1906
                     /locus_tag="IXF54_002328"
     CDS             578..1906
                     /locus_tag="IXF54_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1405558.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIDQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2108..2941
                     /locus_tag="IXF54_002329"
     CDS             2108..2941
                     /locus_tag="IXF54_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1405559.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2957..3694
                     /locus_tag="IXF54_002330"
     CDS             2957..3694
                     /locus_tag="IXF54_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1405560.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3849..4817)
                     /locus_tag="IXF54_002331"
     CDS             complement(3849..4817)
                     /locus_tag="IXF54_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1405561.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5049..5882
                     /locus_tag="IXF54_002332"
     CDS             5049..5882
                     /locus_tag="IXF54_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1405562.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5916..6755
                     /locus_tag="IXF54_002333"
     CDS             5916..6755
                     /locus_tag="IXF54_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1405563.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6781..7341
                     /locus_tag="IXF54_002334"
     CDS             6781..7341
                     /locus_tag="IXF54_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1405564.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7540..9261
                     /locus_tag="IXF54_002335"
     CDS             7540..9261
                     /locus_tag="IXF54_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1405565.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9426..10568
                     /locus_tag="IXF54_002336"
     CDS             9426..10568
                     /locus_tag="IXF54_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1405566.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10584..11795
                     /gene="thiI"
                     /locus_tag="IXF54_002337"
     CDS             10584..11795
                     /gene="thiI"
                     /locus_tag="IXF54_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1405567.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4142 a         2039 c         2525 g         3451 t
ORIGIN      
        1 gaaaaagaaa tccaatcact tgggtttctt tttcttttat tattatgatg aatcaagctt
       61 tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt agagttggct actcataatc
      121 aaccatactt cccatcatcg ctaaatacga aataaatcag cattcctaca ccaatcaaag
      181 cacaagctgc accaagaaaa gggatgacat tcccaaagtt atctgtattc ataagtatgc
      241 caccacccac aaacaaaaag ccgccagcaa tcaggttttt cattttattc atctcctttc
      301 ttacgttata gtatttcctt ctttcaatga gtattttgaa agctatctta ttgtatgatg
      361 ttcttctagg cctcttcttt attatacagg ccattttcgt tatctcatag aaatattcct
      421 ttttatctga aaactattgt ataccgtatg acatttttct tcatgaagtt gtaagtatat
      481 tctcattcaa atgaaaattt catcatagaa gagattgtat ttctaaattc ttaagagtaa
      541 aatacgaagt ataacttgga tttggaagga tgattggatg aaagctatta cgcctgatat
      601 gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc
      661 agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac
      721 accggtattt tctatagatt tggctacggg gaaagtggct aatcaaatgc aatcggggcg
      781 ttgctggatg tttgctgcac tgaacacatt ccgtcataag atgctgaatc aattccattt
      841 aaaagagttt gaattatcac aaaattacac ttttttctgg gataaatatg aaaaggcaaa
      901 ttatttctat gaaaacatca ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc
      961 tttcttactc gctactcctc aacaagatgg cggccaatgg gatatgatcg tttcattatt
     1021 ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa agtgcgaata gttcaaattc
     1081 tcgagatttg aataactact tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa
     1141 aatggttgct caaggagaaa cattagacgc tattgaagaa aagaaagaag aaatgctaga
     1201 aagcatttat aacttcttag ctatctcatt aggaacacct ccaaaagaat tcgattttga
     1261 gtaccgcgat gaagaaaaga actatcatct tgaccgagga ctgacaccac aaatttttta
     1321 tgataaatac atcggtgtaa aattagatga ttatgttagc gtgatcaatg caccaacaaa
     1381 agacaaacca ttcaatcgtt cttatacagt agagatgcta ggaaatgtcg ttggcgggaa
     1441 agaagtcaaa tatctgaatg ttgatatgga aactttcaaa aaattagcga ttgatcagct
     1501 tgaagaagga gaatctgttt ggtttggttg tgatgtaggt caatcttcta ctagaactag
     1561 tggcatcatg gccttagatg cttattcaat ggatgatcta tttgatacgg attttacaat
     1621 gactaaagct gaacgtttag attttggaga aagtctgatg acccatgcga tggtattgac
     1681 cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga
     1741 aaaagttgga aagaatggct tctttgttat gagtgatgcc tggatggacg aatatacgta
     1801 ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga
     1861 accaattgtt ctatctccgt gggatccaat gggtgcttta gcatagacga gtatttcaat
     1921 gaagagtatg tagtaaagat aaaaagagct ggcttaagcc agctcttttt atctttaaag
     1981 tccattccct gttttttatg gtccctgtgc tataattcta aataaaggat aaggattgga
     2041 aataatcctt ttatcattaa aaatatgatg acgaagtaga ataaaattgg gagtaggggg
     2101 gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa ttaaatggta aagtgattcc
     2161 agttgttggt ggcgatgtta atcagaccta tcgtataaaa accgaacatc gtgcttattt
     2221 tttaaaaatc catccgaatg taaaaaaagg attttttgaa gcagaagtag acggattaaa
     2281 agaactgtca gcattcgtac gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg
     2341 cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc
     2401 agctttagct aatctccatc agcaaacagc gccacaattt ggttttcgta aagataacta
     2461 tttgggtaca cttgttcaga aaaattcttt tgaagaagat tggtggactt ttttctttaa
     2521 agatcggctt gaatcccaga tttctttggc agaagaaacg aatcgatgga atgtacagcg
     2581 tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat
     2641 cactccacgc ctcttgcacg gagacctgtg gagtggtaat gtgttctttg accagcaagg
     2701 gcatccagtt tttgtcgacc cagctgtttc ttatgggaat cgggaacaag atattgcaat
     2761 gagccagtta ttcggtggtt tccgaccgga atttttagat gcttatcaaa ctatatttcc
     2821 attagaaaag ggatggaaag accgactgcc gatttaccag ttatattatc ttttggcaca
     2881 tttgaatatg tttggagaaa gttacggttc ccaagtagat caattactag aaaattttta
     2941 agaggagggc gtgtttttgg caaaaaaata taccaaaaag catgaagttt catattatga
     3001 gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc
     3061 tgaagatcaa agcgaccagt tgggaagagg cagtgaattc gtcaatagct ttggacttac
     3121 ttgggtcatc acgaattatt ccatggagat taatcgtctc cctagagtcg gtgaagtaat
     3181 ctcagtaacg actcaagcca tggagtacaa taagtttttc tgttatcgga acttctggat
     3241 ccatgatgaa gatgggaaag aattagtcaa aatagaatct gttttcgtat tgatggattt
     3301 tgtgaatcga aaaatgagca gtgttaatga agagatcatt gctccttttg agagtgagaa
     3361 gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc
     3421 atatcgtgtt cgtttttatg atatcgatag taatcagcat gttaataatg cgatgtattt
     3481 taattggatc attgacgtat taggatatga ctttttgact acgcatgttc cagaaaaagt
     3541 aattattcga tttgataaag aagtagagta cgggaatgag atcgaaagcc attttgaaca
     3601 aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta
     3661 ttgtgaagca aatattatat ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca
     3721 acttcaagaa ataagaagaa atttacgaaa attgtttctc aaatttttgt aaattttcga
     3781 cttagttttc gaagaagcta tttttgtcac agcctctctt tttaagttta gctattagat
     3841 aatgatgttt agttttggat aaacgaaatc aattcttcac ccgacaaacc atagtttaca
     3901 gcaatcaata gttttaaacg tgctttttga ctattaatac tgttacagaa aataatcccc
     3961 attttttcta gttcttttcc cccgccttca taatcataaa caggctctgc gattccattg
     4021 aaacaacgag aaacaagtac tacagggatt ttttggacaa gaagcttttc caaagcagct
     4081 aaggcttgtg gagggagatt ccccgcacct aaagcttcga ttaccaaacc gtctactttc
     4141 gtatgggcga tggcttccaa taagtcccct tgcatcccag catatgcttt aacgatcggg
     4201 atagtcccat ctacagcgga aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat
     4261 aaaattcgat tcttagttac taagccgatg ggacccaatg taggtgttcg gaaagtagca
     4321 acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc
     4381 accaaaaccc ccttatctag cgcttcttca caacttgcga cacgaatcgc actttcaaaa
     4441 ttataaaggc cgtcacttcc aagttcatta cttgaacgca ttgccccagt caaaacaatt
     4501 ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta
     4561 ccgtgtgtga tcaccactcc ggatatacct gatgcataag cggacaagat acgttctttt
     4621 aataccaaca tgtgagaagg catgatgtgt ggtgagggaa gattgaagat atcttcaact
     4681 aataattcta catgcttggg gattgtgatt tctgcatcta ataatgggtt tgccacatca
     4741 ggactaacac cgcccgtgag catgttttct ttcatggcga tcgttccgcc ggtgtgtagt
     4801 acaagtattt ttttcatttt acagacttcc ttacgaataa ttttgcatag ccgttaaatc
     4861 ttattgtata ctatataaaa gagttttgct atcagtgaag aataacgaat agcctaactt
     4921 atcataaaaa agctgacctt attaaaatgg aaaagactca accttgacct cctaatacaa
     4981 tggagtatct ggttgagttt cttttacaag aaagcagcca aggaaatcaa tcacgaaggt
     5041 ggccggaaat gatcagaatc ggattaacat cgtttaaaga acatgacaaa ttaactggta
     5101 aaaagaataa cactctttat gaatatgcag ggtttctacc cttagtggaa ttagatactg
     5161 cctattatgg aattccccga aagtcctcag ttgagaattg gataagtgaa gtacctgaat
     5221 catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg tcaaggagac tggaagcctt
     5281 attatcagac cgaagaagag atgattgcgc attttttaga tgttatgtcg ccaatggtag
     5341 atgctggaaa gatgttttgc tttttagtcc aattcccctc atcattcaaa tgtacgaaag
     5401 aaaatgtcca gtacttgcgc aaacttagaa agtggtttga gggttatcct gtttctatag
     5461 aattgcggga ttattcttgg tacagcgatg aatttcttga acgtacaaga aactttatgt
     5521 ctagagccgc tttttcttta gccatcatcg atgagccaca gttgctgaat acaacgattc
     5581 cttttgatcc atttgtcacc aatccgaact ttgctctttt tcgattccat ggtaggaatg
     5641 cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt
     5701 cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt agttgattct aagacaaaag
     5761 aggtaggtgt gatttttaat aataattctg gtggtgatgc ggctgaaaat gcattagcac
     5821 tgcaaaaaat attaaatatc aagccagatg gactcaatcc aacccaaatg gatctgtttt
     5881 aagggcttat tttattcaag aaaggggcga agtctatgat caaagctatt ttttttgata
     5941 ttgacggaac attggtcacc aatcgttcaa aagcgttgga atcaacaaaa caagcaattg
     6001 aatatgctcg gaaaaacggc attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga
     6061 tcaaagaaat cattgatgaa ttggaattag atatgtatgt cgtgtataat ggtcaactgg
     6121 tttttactgc agatcgcacg attatcgacc acccttttga acaaaaagta ttggaacata
     6181 ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt tggtgcgaga aatcggttag
     6241 atggatcaac gacgatgcta ttaggacaat cgatttttat caagcgacta gtcagctttt
     6301 taccgcgtaa attcccggta cgtctgatga aaaaaatcct ccaggttttt agccctcacc
     6361 gacaaaagga tcgttatgaa acgttagaga tattaaaaga accaatttat caatgtatat
     6421 tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc
     6481 agcgttcaaa tacgtatact gtagatatca tcccaaaagg tggttcaaaa ttactaggca
     6541 tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga aattatggca ttcggcgatc
     6601 attacaatga tatcgagatg ctgaaaggtg taggaattgg agtggccatg gggaatgctc
     6661 agatcgaagt aaaacatgct gcagactttg tgactcaatc aaatgaagaa gatgggatat
     6721 acgaaggact gaaacacttc ggtctgattt attagtcaat gaaagaagga aaaaatcagt
     6781 atggaagcag agacaccttt tcaaaaagta gaaacattcc acaaaacttt tgatccaaga
     6841 agaccagaga tacccacggc ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa
     6901 atagaagaac ttgttgaatt tgttgcagct acttcaccta ataaagaaca actgcatgaa
     6961 gctgttatgc tgctgcacga agcattagat aaagcagaag aaaaaatact tggtaaaaca
     7021 cagtggggaa gtgatccgct ggttgaacaa gtagatgcac tgacagatat cctttacttt
     7081 acttatggtt ctttttcatt actaggaata gtgcctgatc gaatattcga aatcgtccat
     7141 caggcaaata tggggaaatt atttccagat ggaaaacctc attatgaccc tgtaacaaat
     7201 aaagtgttga aacctgaaaa ctggcagatg gaatatgctc cagaacctaa aatcgcggca
     7261 gaaatccaac gtcagattca agaagcgatt gctaagaaga aatccgctga tataggcatg
     7321 tgcacaaatg aaaaagagtg atcagagttt cactattttt gttatcttta tacgatggaa
     7381 aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa tagtatgcta taatctagaa
     7441 tagatgtctt ttgcacgaga gacgtattat ttggatttat gccaagcaga acgccatctg
     7501 ttgcataaaa gatgcatact gaaaacggag gaaccataaa tgggaaataa tgttatcatc
     7561 ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct ttttggttag ttacttgatg
     7621 aagaaaaaaa accaagatcg attggatgcc ttagaaaaac gcaaggaaga attatttgac
     7681 ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa
     7741 aatacctttc gtgaatggag tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa
     7801 ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga
     7861 aaaaaagcag ttgttgaagc agaagctaca atggaccaaa tggagaaaca agtgacggaa
     7921 atccgtgaag gtctaaagga attgcgggaa acggaagaac gcaactcact agctgttcag
     7981 caggcacttg acgtctatga agaactgaaa gaaaaagtca gagaagacga gcatgaatac
     8041 ggaccagctc tttttgaatt gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag
     8101 tttgtgactt taaacacttc tggagaccct gttgaagccc gcgaggtttt ggataaagca
     8161 gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag
     8221 ctccatacca ctttccctga acaacttgaa gaaatcagtg atacttacga gaaattgatg
     8281 gaagaacaat atgtattccc agaagaagat cttgctgaag atatcgcaaa agtaagtcga
     8341 agaattgaaa attcattagc caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa
     8401 aaccgtgaaa cagctgattt gattgattcg ttatatgata tcttagaacg ggaaatggaa
     8461 gctcagagat atgtaaaaac taaccaatca acgattgcag agtatatcaa acatacaaca
     8521 aagaataacc gtcaattatt gattgagctg gatcacacat ctcaaagcta tacattgaac
     8581 cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa
     8641 aatgaacaga tgattccaca gatccgtcaa cacgaaattc cttattcaga agtgcgcacg
     8701 ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa agcaacaagt agagatcgac
     8761 gactcattac atgaattgag aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc
     8821 gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg
     8881 aatgattacc tagaattctt ttttgttgct actgaccgta ttgaagaact aagtgttgtc
     8941 ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt tagttgctct atgtgaagaa
     9001 gatctagaat tattagataa gaagacacat gatttagtag atgcagcagc attaacggag
     9061 caaatgctcc aatatgcaaa tcgttatcgt catacacacg aagaagtgag agaagcaatt
     9121 gataaaagct actatttatt caataaagaa tatcactatc aagaagcatt agacgaaatc
     9181 ggtacagcat tagagcgagt agaacctggt gctttcaaac gcatcgaaga tttttatttt
     9241 aatcatccag acttagtata aagcaaaaaa gttagtaaac cagcaagagc aagacggatt
     9301 attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga aaatttcacc
     9361 tgctcttgaa agcaccttgg acaaccgtta taatagaaaa gaatttcaaa aaagaggggt
     9421 ttcttatgat atattttgat aacagtgcaa caaccccaat ctttccgcaa agcttagatg
     9481 catatgtaaa aaccagtcag cggattattg ggaatccatc aagcttgcat gatttaggca
     9541 atcaagctaa ccggttgttg cagcaagcaa gaaagcagat cgctgatttg atccatgtag
     9601 aaccagaaga aatctatttt acttcaggcg gaacagaagg agataactgg gtattaaaag
     9661 gaacgatgat cgaaaaaaga gaatatggga atcatatgat catttcagga gtcgaacatc
     9721 cggccgtcag cgagacagca gaacaattaa aagaattagg aatcgaagta agtatcgccc
     9781 cagtggataa acgcggattt gtacgtgtcg acgaattgaa agaattaata agaaaagaga
     9841 cagtattagt ctctgttatg gctgtgaata atgaagtagg atcgattcag cccatccagg
     9901 aaatcagtga tcttttggat gattttccta aaattcattt ccatgtagat gctgttcagg
     9961 cgattggaaa agtaccattc tcgcaatggt tgacagaaag agttgatttt gcaacatttt
    10021 cagctcataa attccatggc ccacggggaa ctggtttcat ttattggaaa aagggtcgtc
    10081 gtttagcacc attactgaca ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa
    10141 atgttccagg tattgtggct atggttaaag ctttacgttt atcattagag ataaaggaac
    10201 aaaaacctga gcagacacaa cgcttaaaag tagctctaat caatgcgttg aagacgtatg
    10261 agaaagtgac gattttttca gaaggaactg actatgcacc gcatatttta tgttttgctc
    10321 taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa atatttgttt
    10381 ctactactag tgcctgctcc tcacgtaaac atatggcaag cagtacattg cacgcaatgc
    10441 atgtaccaaa tgatctggct acatctgctg ttcgaatcag cttagatgaa aacaattcgc
    10501 ttgcagaagt cgagcaattt atgattattt tcaagcagct ttataagaaa ttttctgcaa
    10561 tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt tatggggaac
    10621 tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca actagcacaa aatgtcagac
    10681 aagctttgaa agactttcca gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt
    10741 tattgaatgg cgcagacggg cagctagtta ttccaaaact agcaaaaata tttgggatcc
    10801 aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa caagctgttc
    10861 aagacatcat gaaagaaatt tatactggga aagaaacatt taaaattatc gctaaaagaa
    10921 gcgatcacca atttgaattg acaagtaatg aattgaatca aacattaggt aatgctgttt
    10981 ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc agatattcca ttacgtgtag
    11041 agattcggcg tgatggcgct tatctatcgt atgaaacgat caagggagct ggaggattgc
    11101 ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg ggggattgat tctcctgtag
    11161 cagggtactt tgccatgaag cggggagtgg aaattgaagc tgttcatttt gctagtccgc
    11221 cgtatacaag tgaacaagca ctgcaaaaag caaaagatct aacagccaaa ctagcacctt
    11281 atgtgggaac gatccatttt atcgaagtgc catttaccga gattcaagaa gaaatcaaaa
    11341 agtcagtgcc acaaggatat tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg
    11401 atgaaattcg ccgtattcgt catggtttag tcattttgaa tggtgaatca ttaggtcaag
    11461 tggcttcaca aactttgcaa agtatggtgg ctatcaatga agtaacgaat acgccaatca
    11521 ttcggcctgt agcgacaatg gataaacttg aaatcattga agtagcagaa cagatcgata
    11581 catttaatct ttctatccag ccttttgaag attgctgtac gatttttgct cctccacagc
    11641 caaaaacacg ccctcaactg gaaaaggtcc atcaatatga agagcgttta gcaatagatg
    11701 aaatgatcca acgagcacta gctggcttga agatggaaga gatacaagca gaatcgtcta
    11761 atgaaaaaac tgaagaattt tcagaattgc tataagaggt tatgaaaata gtgttttgac
    11821 cagagtatta gagaaacaaa acgaaaaaat agctccatgt attttaccgt ttttgaacta
    11881 atgtcgaaga ttagctgatt gaataccatt tatcaagaag ttatggaaaa gccggttctt
    11941 ttttactaac tgaaaaataa aattttattt agagatcaag ctacagttga taaatgatat
    12001 aaaaaagaga atttcccatg aaatagcgag gttgtgacag aagtgggcag cttcaagaaa
    12061 taagaaggaa tttacgaaaa ttgcttttca aatttttgtg aaatttcggc ttatttttcg
    12121 aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACERL010000078        11337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.083, whole genome shotgun sequence.
ACCESSION   DACERL010000078 DACERL010000000
VERSION     DACERL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.083"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(366..1484)
                     /locus_tag="IXF54_002338"
     CDS             complement(366..1484)
                     /locus_tag="IXF54_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405568.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="IXF54_002339"
     CDS             1955..3262
                     /locus_tag="IXF54_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1405569.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="IXF54_002340"
     CDS             3381..4580
                     /locus_tag="IXF54_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1405570.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="IXF54_002341"
     CDS             complement(4607..5875)
                     /locus_tag="IXF54_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1405571.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="IXF54_002342"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="IXF54_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="IXF54_002343"
     CDS             complement(7092..7595)
                     /locus_tag="IXF54_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1405572.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="IXF54_002344"
     CDS             complement(7718..8482)
                     /locus_tag="IXF54_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1405573.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="IXF54_002345"
     CDS             8590..8865
                     /locus_tag="IXF54_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1405574.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="IXF54_002346"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="IXF54_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1405575.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10632)
                     /locus_tag="IXF54_002347"
     CDS             complement(10432..10632)
                     /locus_tag="IXF54_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405576.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3400 a         2387 c         2002 g         3548 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaa
//
LOCUS       DACERL010000079        10783 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.084, whole genome shotgun sequence.
ACCESSION   DACERL010000079 DACERL010000000
VERSION     DACERL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.084"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            28..3117
                     /locus_tag="IXF54_002348"
     CDS             28..3117
                     /locus_tag="IXF54_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1405577.1"
                     /translation="MSLFYLKEKLEARIEELGKYRFSKIVELSDWLVVEDETKKAKYP
                     PKDFSSAATFSVGDTWKGRDFYLWVKKEIEMPDVDDLYLYLNFGHTGGGYNSGFESLM
                     FINGKPYQGVDSNHKEVKIADEFRGKKVEVALKLWSGLEGGGPEQIQVHQFKQAELSQ
                     MMVPVDELFYYADAINKTVKELGQDNPIQYKLSHVLNHAINQINWIEPGSQTFYTSVA
                     KAVSELSEGIAAIPKDEMITVTAVGHTHIDVAWLWRLKHTREKAARSFSTVLRLMEEY
                     PEYIFLQTQPQLYKYIKEDYPEIYAKIKERVAEGRWEADGSMWLEADCNIPSGESLTR
                     QILHGAKFIKEEFNQDIHYLWLPDVFGYSWALPQILTKSGIDTFMTTKISWNQFNRMP
                     HDTFKWKGMDGSEVLTHFITTPEPGKENSWESNWYYTYNGELEPETVLGVYNAYRDKN
                     INDELLISYGFGDGGGGVNRDMLEKRRKLDKIPGLPKVQTGKAVDYFDRLQETFKNTD
                     EYVHTWDGELYLEYHRGTYTSQAYVKKANRQSELLLRQLEILFSLMEMKGQEAYPKVE
                     IFNMWETVLRNQFHDIIPGSSIKEVYQDHRIEMEGLFKEAQSLLAPLQSVGDSQTVCN
                     FANWQRSILAELPFLLDNQVYVDEAGEVIETYANGEVLYGLISNVPALGQKMIHVTEK
                     QEMAQIPTVAVVDSNQIETDFYLIQWNKSGQLTSVFDKELKREVLSGTGNVLQLFEDK
                     PMNFDAWDIDIYYQEKFQELAAQEIIVKPANALYQCVEFIYRFGESTIKQEMRLYPHT
                     RRIDFVTDIDWQERQQLLKAKFDVAVRATYATYDIQYGNVRRANNWNTSWEMAKFETV
                     AHQWMDMSQRDFGVSILNDSKYGCDVKDQTMRLSLLKGAIHPDPTADMGQHHFTYSLY
                     PHRGDFIEGKTIQEAWEINEKPLVLNGSIGTLPQITTTQDATIVIDTIKKAEDGNGWI
                     IRAHEYAGTDQTVDLTIEGLTWWRETDLLERNIGEVKDKQPSLTFAPYEIKTIRFGK"
     gene            3086..4453
                     /locus_tag="IXF54_002349"
     CDS             3086..4453
                     /locus_tag="IXF54_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HAQ1405578.1"
                     /translation="MKLKQFVLGNEGRALEITAEFCQKLKSNYEGTPKYKLAELGVVR
                     NGVVESAENVSAKSQNPFVFSIEVPTLKIPQQYHSGRCWLFAIVNLLNQQISQKLEIA
                     EFELSQNYLYFYDKLEKANFFYKNIIETADLELGSRELDFLLSKPQEDGGQWDMLVSL
                     IEKYGIVPSYSMPETENSRSSADMNKYLNAKLRLDAKKLRQMVAEDCSQKDIDEVTSQ
                     MLAEVYRMLAICIGAPPEKIDMKYRDDAGNYHAGLNLTPKEFANKYLLMNLKDYIPII
                     HAPSKNKVYNQTYTVAMMGNIIEGQPIKYLNVDMATLKALTIKQLKDHEAVWFGSDIG
                     QYTDRKLGIMATDIYAVDNLFDVDFTSNKQDRLEYCQSLLTHAMVISGVDLQNDQPTI
                     WKVQNSWGEEIGYKGFFMMTDAWLEEYVYQVVINKKYLTKEQLAKYQEEPVILPPWDP
                     MGSLA"
     gene            4528..5346
                     /gene="iolB"
                     /locus_tag="IXF54_002350"
     CDS             4528..5346
                     /gene="iolB"
                     /locus_tag="IXF54_002350"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ1405579.1"
                     /translation="MNLLQKPILFPVNEKVQLIQYITGENSVLEHVELSVYDVEAGGE
                     VSYHEAALESCVVVLSGQVSVAILNGETYPKLGTRTSVFDKIPTHSVYIGKNRSYKVT
                     AETKARILIAASKTDQSLPDRLIKPEDVLLESRGAYQNKRYVQTILSDQDTISDKLLV
                     VEVYTEGGNFSSYPPHKHDQNRLPEESFLEETYYHEINPPQGFVFQRIYSDDRLLDQT
                     MSCSNQDVVIVPKGYHPVGVPDGYTSYYLNIMAGPTKMWRFYDDPDHEWIKNRS"
     gene            5396..6373
                     /gene="iolC"
                     /locus_tag="IXF54_002351"
     CDS             5396..6373
                     /gene="iolC"
                     /locus_tag="IXF54_002351"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ1405580.1"
                     /translation="MKKFAITALGRACIDLNSVEINQPMEETKTFKKYVGGSPANIAI
                     GLAKLGETVGFIGKLSNDQHGRFIKDFMSKAGVDSSQMIFDQEGHKSGLAFTEIKSPS
                     ECSILMYREDVADLYLTPDEVSEEYIEASEMLLISGTALAQSPSREAALLAVEYAHKH
                     DVKVIFELDYRPYTWGSTAETAVYYTLLAQKAHIVIGTRDEFDVLENSVGGNNQQTVE
                     RLFQYTPELILIKHGVDGSFAYEKDGTITRGYAYKAQVLKTFGAGDSYAAAFLFALMQ
                     GKMIENALQYASASSALVVSRHSSSEAMPSVSEIEAVIAEQSWGEKVHA"
     gene            6366..8282
                     /gene="iolD"
                     /locus_tag="IXF54_002352"
     CDS             6366..8282
                     /gene="iolD"
                     /locus_tag="IXF54_002352"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ1405581.1"
                     /translation="MPKTLRLTTAQALVKYLNQQFLIVDDQKLPFVKGIFHIFGHGNV
                     LGLGQALSQNPGHLEIYQGKNEQGMGQAAVSFAKEKLRRQIFAVTTSVGPGAANLVTV
                     AGTALANNLPVLLLPGDTFASRQPDPVLQQVEHFHGQGITTNDALKPLSRYWDRIERP
                     EQLMSALTRAFEVLTDPVTAGPVTICLPQDVEGEAYDYPEDFFNEKVHYFDRRLATDR
                     ELSEAVKLIERSLKPVIIVGGGAKYSEAGSALKRFSEKHKIPLVETQAGKSTVVADFP
                     LNFGGVGVTGGAPANRLVKEADLVIGLGTRYTDFTTASKTAFDKDQTTFLNINVSRMQ
                     SYKMNGVSVVADAKTALLQIESELDDLYQTQYGDLLTSVTRDWQNERERLANLNYHDA
                     DFEMEIKNHFGAEKLANYAQQLTTELTQTAALIKINQSLPLDSIVVGAAGSLPGDMQR
                     LWKAKTVNSYNMEYGYSTMGYEVAGAYGAKIAHPDKEVFAFVGDGSFHMLHSELLSSI
                     QYGKKINVLLFDNSGYGCINNLQMGNGMPSFGTELTDYHEEIMTVDFAKIAEAYGAVA
                     YNVGNMSELEKSLAEIAESDRSTLIHIKVLPKTMTDGYDSWWNTGIAEVTADGSTEKV
                     LAEKEEKLAGAFQY"
     gene            8304..9458
                     /locus_tag="IXF54_002353"
     CDS             8304..9458
                     /locus_tag="IXF54_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ1405582.1"
                     /translation="MYQLTLPQKIIYGDQAIKETGKIAGNLGLKTLIITDKLIVSLNL
                     LEGVLASLKEENLTWKIFDGVDREPTDQIVAEALAFYQQEDYDSIIAVGGGSCIDTAK
                     AVAVLINNSGSYAEFASGQLIAQNPLPLIAIPTTAGTGSEVTDVTVITDTQKDIKYMM
                     KQAAFLSYAAIVDPQMSRTSPASVTAATGLDALCHALESYISKKAQPITRLFSLSAIK
                     LIMENLPIAYSEPENLIARENLAQAAMEAGIAFSNASVTLIHGMSRPVGALYHIPHGI
                     SNAILLEAVIDYTKPVIISELAGIAKYLNPEKEGTDQELAECFFQELQDLIEKLAVPN
                     YASFGIEKAAFIASLDKMANDAIASGSPNNNPLVPTIEEIKMIYLASWQE"
     gene            9481..9912
                     /locus_tag="IXF54_002354"
     CDS             9481..9912
                     /locus_tag="IXF54_002354"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405583.1"
                     /translation="MEIKTDLNSEDSSQLAEFLLMVLDQQQEHFFPDYQTIDKSVALA
                     AYSGTELVGGLLGKISDQSLNVSLLAIDPKHQKDGLGSQLIEEVEKIAAKNQVINLLL
                     STRSYQALAFYQKNNFEIYGELKDMPFRGVDTYYLVKRLMA"
     gene            9950..>10783
                     /locus_tag="IXF54_002355"
     CDS             9950..>10783
                     /locus_tag="IXF54_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1405584.1"
                     /translation="MTYKEIPVSVQQFMEKITTLCGNDHPQWAANINAAFANTLLTTV
                     KRHEDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKDNPDLADMISGLVKKQFFYINLD
                     PYANAFNEEANNAGHQDDETQMNPWVWERKYEIDSLCYPIQLAYLLYQNTGRTDQFSE
                     DFIRGIEKVLTVFETEQNHQDSPYTFQRFDDRPEDTLVNHGRETTVAPTGMTWSGFRP
                     SDDACHYGYLVPSNMFAVVILDYLVEIFQDILKETTLIDRIIKLKKRSMMVSKNLVRF
                     LI"
BASE COUNT         3391 a         1886 c         2441 g         3065 t
ORIGIN      
        1 aaaatgaaaa gggtggagga atagttcatg agtttgtttt acttgaaaga aaaattagaa
       61 gcaagaatcg aagagttagg aaaatatcgc ttttcaaaaa tagtagagtt gtctgattgg
      121 cttgttgtag aagatgagac caaaaaagca aaatatccac ccaaagattt ttcaagtgct
      181 gctacttttt cagtagggga tacttggaaa ggccgcgatt tttatttgtg ggttaaaaaa
      241 gaaatcgaga tgccagatgt tgacgattta tatttgtatt tgaattttgg tcataccggt
      301 ggtggttaca actccggctt tgaatcattg atgttcatta atggaaaacc ctatcaaggc
      361 gtggattcca atcataagga agttaaaatc gccgatgaat tccgtggtaa aaaagtcgag
      421 gtagcattga agctttggtc aggtttggaa ggtggcggac cggaacaaat ccaagtccat
      481 caatttaagc aagcggagct atctcaaatg atggtgccag ttgatgaact attttattac
      541 gctgatgcta tcaataagac cgtgaaagaa ttaggtcaag ataacccgat tcaatacaag
      601 ttgtcccatg ttttaaatca tgctatcaac cagattaatt ggatcgaacc tggaagccaa
      661 acattttata cttcggtagc gaaggctgta tccgaattat cagaggggat tgccgcaatc
      721 cctaaagatg aaatgatcac tgtgacagca gtcgggcata cccatattga cgtagcgtgg
      781 ttatggcgat taaaacatac ccgcgaaaaa gctgcccgta gcttctcaac tgttttacgt
      841 ttgatggagg agtatccgga gtatattttc ttacaaacac aaccgcaatt atacaaatat
      901 atcaaagaag actatccaga aatttatgca aaaatcaaag aacgagtagc tgaaggtcgt
      961 tgggaagcgg atggaagcat gtggttggag gcggattgta acattccttc tggtgaatct
     1021 ttgacccgcc agattttaca tggtgcgaaa tttattaaag aagaattcaa tcaagacatt
     1081 cactatttgt ggttgccgga cgtttttggc tattcttggg cattgcctca gattttgact
     1141 aaaagtggca ttgatacttt tatgacgaca aagattagtt ggaatcagtt taatcggatg
     1201 cctcatgata ccttcaaatg gaaagggatg gatggctcag aagtcttgac ccattttatt
     1261 accacgccag aaccaggcaa agagaattca tgggaatcca attggtacta tacctataat
     1321 ggtgagttag aacctgaaac tgttttaggc gtttacaatg cttatcgaga taaaaacatt
     1381 aatgatgagt tgttgatttc ttatggattt ggcgatggcg gaggcggcgt taatcgtgat
     1441 atgttggaaa aacggcggaa attagacaaa attcctggct taccgaaagt tcaaaccggt
     1501 aaagcagtgg attacttcga tcggctgcaa gagaccttta aaaatactga tgaatatgtt
     1561 catacctggg atggcgaatt gtatttagaa tatcatcgag gcacctatac ctctcaagct
     1621 tatgtcaaaa aagcgaaccg ccaaagtgaa ttattgttgc gccagttaga aatcctcttt
     1681 tcattaatgg aaatgaaggg acaagaggcc tatcccaaag ttgaaatctt taatatgtgg
     1741 gaaacggttt tgcgtaatca attccacgat attatcccgg gatcttcaat taaagaggtt
     1801 tatcaagatc atcggattga aatggaagga ctttttaaag aagcccaaag tttattagca
     1861 ccattgcagt cagtaggtga cagccaaaca gtttgtaact ttgcaaactg gcagcgttca
     1921 attttagctg aattaccatt tcttttggat aatcaggttt atgttgatga agctggtgag
     1981 gtaattgaaa catacgctaa tggtgaagtg ctttatggat taatttcaaa tgttccagct
     2041 cttggtcaaa aaatgattca tgtcactgag aagcaggaaa tggcgcagat tcctacagta
     2101 gctgttgtgg attcaaatca aattgagaca gacttttatt tgatccagtg gaataaaagc
     2161 ggacaattga cttcggtatt cgataaggag ttaaaacgag aagttttaag cggaaccggg
     2221 aatgtcctgc aattatttga agataaaccg atgaattttg atgcttggga tatcgatatt
     2281 tattatcaag aaaaattcca agaactagca gcacaagaaa ttatcgtcaa acccgctaat
     2341 gctttatatc aatgtgtcga atttatttat cgttttggtg aatcaaccat taaacaagag
     2401 atgagattat atcctcatac tcgtcgcatt gattttgtga cagatattga ttggcaagag
     2461 cggcaacagc tgctaaaagc taaattcgat gttgctgtca gagcaactta cgcaacttat
     2521 gatattcagt acgggaatgt tcgtcgtgcc aataactgga atacgagctg ggaaatggct
     2581 aaattcgaga ctgtcgccca tcagtggatg gatatgtcac aacgtgattt tggggtcagc
     2641 atcttaaatg attccaaata tggttgcgac gttaaggacc aaacaatgcg tttgagtttg
     2701 ttgaaaggag caattcatcc tgatccaacg gcggatatgg gccaacacca ttttacctac
     2761 agtctgtatc cacatcgtgg cgactttatt gaaggaaaga ccattcaaga agcttgggaa
     2821 atcaatgaaa aaccgcttgt tttaaatggt agtattggca ctttaccgca aattaccaca
     2881 actcaggatg cgactatcgt aattgatacg attaaaaaag ccgaagatgg taatggttgg
     2941 attatccggg cccatgaata tgccgggact gatcagacgg ttgacctgac aattgaaggt
     3001 ttaacttggt ggcgtgagac ggacctgttg gaaagaaata ttggcgaagt aaaagataaa
     3061 caaccgagtt tgacatttgc accatatgaa attaaaacaa ttcgttttgg gaaatgaggg
     3121 aagagcattg gaaataactg cagaattttg tcaaaagtta aaaagtaact acgaaggaac
     3181 ccccaaatat aaactagcag aattaggtgt tgttcgaaat ggtgttgttg aatcggcaga
     3241 aaatgtctca gctaaaagtc agaatccctt tgtgttttca atcgaagtac cgaccttaaa
     3301 aattcctcag caatatcatt cgggacgctg ttggctgttt gcgattgtca atttactgaa
     3361 tcagcaaatc agtcagaagt tggaaatcgc tgaatttgaa ctttcgcaaa attatctata
     3421 tttttatgac aagttagaga aagccaactt tttctataag aatattattg agacggctga
     3481 tttagagttg ggcagtcgtg aattagattt tctactatct aagccccaag aggatggtgg
     3541 tcaatgggac atgctagttt cattgattga aaagtatggg attgttccat cttattcgat
     3601 gccggaaacc gaaaatagtc gcagttcagc tgacatgaac aagtatctaa atgccaagtt
     3661 gcgacttgat gcaaaaaaac tgcgtcaaat ggtggctgag gattgttcac aaaaagacat
     3721 tgatgaagtg acttcccaaa tgctcgcgga agtttaccga atgttggcaa tctgtatcgg
     3781 ggctccgccg gaaaagattg atatgaagta tcgtgatgat gctggaaatt atcacgctgg
     3841 attaaatcta acaccaaaag aatttgccaa taagtatttg ttaatgaatt taaaggatta
     3901 tattccgatc attcatgcac cttccaaaaa caaagtgtac aaccagacct acacagtagc
     3961 gatgatggga aatattatcg aaggacaacc gattaaatat cttaatgtag atatggcaac
     4021 gttaaaagca ttgacaatca agcaactgaa agatcatgaa gccgtctggt ttggtagtga
     4081 tatcggccaa tatactgacc gaaagctagg gattatggcc acagacatct atgctgttga
     4141 taatttattc gatgtcgatt ttacttcaaa taaacaggac cgtttagaat actgtcaaag
     4201 tcttttgact catgccatgg taatcagcgg agtagatttg caaaatgatc agccgacgat
     4261 ttggaaagtc cagaatagct ggggcgaaga aatcggttat aaaggctttt tcatgatgac
     4321 cgatgcttgg ctagaggaat atgtctatca ggtggtgatt aacaaaaaat atttaacaaa
     4381 agagcaactg gcaaagtatc aagaggaacc agttatttta ccaccctggg atccgatggg
     4441 ttctttagca taataaaaaa accgcggcta gtcataacga gatgattgtt tgtgaccggc
     4501 ccccgatttt agtaaagagg gaaaataatg aatcttttac aaaaaccaat tttatttcca
     4561 gtgaacgaaa aagttcagtt gattcaatat ataaccggag agaatagcgt ccttgagcat
     4621 gttgaattaa gtgtttacga tgtcgaagct ggcggtgagg ttagttacca tgaagctgct
     4681 cttgaaagtt gcgttgttgt tctttccggc caagtgtccg tagcgatttt aaatggagaa
     4741 acctacccta agcttggaac gagaacttca gtttttgata aaataccgac tcacagtgtt
     4801 tatattggta aaaatcgttc atacaaagtt acggcagaaa cgaaagcgcg tattttgatt
     4861 gctgcttcta aaacggatca aagtctacca gatagattga tcaagcctga ggatgtctta
     4921 ttggaatcac ggggtgctta ccaaaataaa cgctatgtgc agacgatctt gtctgatcaa
     4981 gatacaatca gcgataaact tttagtcgtg gaagtttaca cagaaggtgg taatttttct
     5041 agctatccac ctcataaaca cgatcaaaac cgcctaccgg aagagtcttt cttagaagaa
     5101 acttattatc atgaaatcaa tccaccacaa gggtttgttt ttcaacggat ttattccgat
     5161 gatcgtcttt tagatcaaac catgtcctgt tccaatcaag acgtggtaat cgttccaaaa
     5221 ggctatcatc ctgttggcgt accagatggg tacacttctt attatctaaa tatcatggcc
     5281 ggtccaacga aaatgtggcg tttctatgat gatcctgatc acgaatggat caagaaccga
     5341 tcgtaagagt ttttcagaaa aataaaacgt gtcatgagag aggaagaaaa ttaaaatgaa
     5401 gaaatttgct attacagctt tagggagagc ctgtattgat ttgaactcag tagaaattaa
     5461 ccaaccaatg gaagaaacaa aaacatttaa aaaatatgtt gggggatctc cggcaaatat
     5521 tgcgattggt ttggccaagt taggggaaac agtaggtttc atcggaaaac tttctaatga
     5581 tcagcacggt cgatttatta aagacttcat gtcaaaagcg ggtgttgatt caagtcagat
     5641 gatttttgat caggaaggac ataaatccgg actggctttt accgaaatta agagtccatc
     5701 agaatgcagt attttgatgt atcgtgaaga tgtggctgac ttatatctaa cccctgatga
     5761 agtttcggaa gagtacattg aagccagcga aatgctattg atttcaggaa cagctttagc
     5821 ccaaagtccg tcaagagaag cagcgttatt ggcggtggaa tatgcccaca aacatgatgt
     5881 aaaagtgatt tttgaattgg attatcgtcc ttatacgtgg ggatcaactg ctgaaacagc
     5941 cgtttactac actttgctag cacaaaaagc ccacattgtg attggtaccc gcgatgaatt
     6001 tgatgtgtta gaaaatagtg ttgggggtaa caaccaacaa actgttgaaa gacttttcca
     6061 atatacgcct gaactgatcc tgattaaaca cggggtcgat ggctcatttg cttatgaaaa
     6121 agatggcacg attacgcgag gatatgccta taaagcacag gttttgaaaa cctttggggc
     6181 aggtgattct tatgcggccg ctttcttgtt tgctttgatg caaggaaaaa tgattgagaa
     6241 tgctttacag tacgctagtg cctcgtcagc gctagtagtt agtcgtcata gttcttcgga
     6301 agctatgcca agtgtgtccg aaattgaggc agtcattgca gaacaaagct ggggggagaa
     6361 agttcatgcc taaaacgcta cgcttaacca cggcccaggc attggttaag tatttgaatc
     6421 aacagttttt gattgtagac gatcaaaagc taccctttgt gaaaggtatt ttccatatct
     6481 ttggccacgg aaatgtctta ggattgggac aagctttgtc acaaaatcct ggtcatttgg
     6541 aaatctatca aggaaaaaat gaacagggaa tgggccaggc ggctgtttcc tttgcaaaag
     6601 aaaaactgcg ccgtcaaatt tttgcagtaa cgacttcagt gggaccgggt gctgcgaatc
     6661 ttgttacagt agctggcact gccttggcta ataatttgcc agtattatta ttgccgggag
     6721 atacttttgc aagccgtcaa ccagatccag tcctgcagca agtcgaacat tttcacggtc
     6781 aaggaatcac gacaaatgat gcattgaagc cactttctcg ttattgggat cggattgagc
     6841 gcccagaaca attgatgagc gcattaaccc gggcgtttga agttttgact gatcctgtga
     6901 ccgcgggtcc cgttacaatt tgcttacccc aagatgtcga aggggaggct tacgattacc
     6961 cagaggattt tttcaatgaa aaagttcatt attttgatcg ccgcctagct acagatcgtg
     7021 aattatcaga ggcggttaag ttaattgaaa gaagtctgaa accagtgatt atcgtgggtg
     7081 gcggggcgaa gtattcagaa gccggatcag ctcttaaacg tttttcggaa aagcataaga
     7141 ttcctttagt ggaaacccag gcaggaaaat caacggtggt tgctgatttt ccattgaatt
     7201 ttggtggtgt cggtgtgacc ggtggtgcac ctgccaatcg tcttgtgaaa gaagctgatc
     7261 tagtgattgg gttgggaaca cgctacaccg attttacgac agcctcaaaa acggcttttg
     7321 ataaggatca aacgactttt ttaaatatca atgtttcgcg gatgcaaagc tacaaaatga
     7381 acggtgtttc agtggtagct gatgccaaaa cggccctatt gcaaattgaa tctgagcttg
     7441 atgatttata tcaaacgcaa tatggtgacc tgttaactag tgttactagg gattggcaga
     7501 atgaacggga gcgtctggca aatttgaact accatgatgc tgattttgag atggagatca
     7561 aaaatcattt tggcgcagaa aagttagcca attatgcgca acaactaaca acagaattga
     7621 ctcaaaccgc ggctttaatc aaaatcaatc aaagtttgcc gctggatagt atcgtggttg
     7681 gggcggctgg ctctttgccc ggtgatatgc aacgtctgtg gaaagcgaag acagttaact
     7741 cttataatat ggagtatggc tattctacga tgggatatga ggtagcgggt gcttatggtg
     7801 ccaagattgc gcatccagat aaagaagttt ttgcttttgt tggagatggt agttttcata
     7861 tgttgcattc ggagctactt tccagtattc aatatgggaa aaaaatcaat gtcttacttt
     7921 ttgataactc aggctatgga tgtattaata atttacaaat gggcaatggg atgccatctt
     7981 ttggaacgga actgacagat tatcatgagg agatcatgac ggttgatttt gcaaaaatag
     8041 ccgaagctta tggggcggtt gcttataatg tcggtaatat gtctgaactt gagaaatctc
     8101 tagctgaaat agctgagtct gaccggagta cattaatcca tattaaagtt ttacctaaaa
     8161 ctatgacgga tggatacgat tcatggtgga ataccgggat tgctgaagtt actgcagatg
     8221 gttctactga aaaagtttta gctgaaaaag aagaaaaact agcaggggcc tttcaatact
     8281 gagaagccca gaaaggtgtc aaaatgtatc aactaacttt gccgcaaaaa atcatttacg
     8341 gcgatcaagc aatcaaggaa acaggaaaaa tcgctggtaa ccttggtttg aaaactttga
     8401 ttattacaga taaactaatc gtctctttga accttttgga aggtgtgctt gccagcctaa
     8461 aagaggaaaa tctgacctgg aaaatttttg atggcgttga ccgcgagcca acggaccaga
     8521 ttgtagctga agcattagct ttttaccaac aggaagatta tgattcgatt attgcggtgg
     8581 gcggtggcag ctgtatcgac actgctaaag ctgtggctgt gttaatcaat aatagtgggt
     8641 cctatgctga atttgctagt ggccaactga ttgcccaaaa tccactgcct ctaattgcaa
     8701 tacctacgac tgccgggact ggctccgaag tgacagatgt gaccgtaatc acagatacgc
     8761 aaaaagatat taaatatatg atgaaacagg ctgctttttt atcctatgca gcgattgttg
     8821 atccacaaat gagtcgtaca agtccagcat cagttacagc agcaaccgga ctagatgcat
     8881 tatgtcatgc tttagaaagt tatatttcta aaaaagccca gcctattaca aggctctttt
     8941 cactttcagc aataaagttg attatggaaa atttaccgat tgcctatagc gagccggaaa
     9001 acttaatagc ccgtgaaaac ttggcgcagg cagcaatgga agcgggaatt gccttttcaa
     9061 atgcttcggt taccttgatt catgggatgt caagacctgt tggcgctctg tatcatatcc
     9121 ctcatggcat ttccaatgcg attttattag aggcggtgat tgattacaca aaacctgtaa
     9181 tcatctcgga gttggctggt attgcgaaat atcttaatcc tgaaaaagaa ggtacggatc
     9241 aggagttggc agagtgcttc tttcaagaat tgcaggactt gattgagaaa ttagctgttc
     9301 ccaattacgc aagttttggt attgaaaagg cagcttttat tgcatctttg gataaaatgg
     9361 caaatgatgc aattgccagt ggtagcccca ataacaatcc tttggtacca acaattgagg
     9421 aaataaaaat gatttattta gctagttggc aagaatagtc aaagaaaagt aggtagaaaa
     9481 atggagataa aaacagatct taattcggaa gattctagtc agttggctga gtttctttta
     9541 atggtcttgg atcaacagca ggaacatttt ttccctgatt atcaaacaat cgataaatct
     9601 gtggccttgg ctgcttattc aggaactgaa ttggtaggcg gtctgctagg gaaaatcagc
     9661 gatcaaagct tgaacgtttc gttattagcc attgacccga aacatcaaaa ggatggtcta
     9721 ggaagtcagt tgatcgaaga agtcgaaaaa attgcggcaa aaaaccaagt cattaatctt
     9781 ctactttcga cgagaagtta tcaagcgctg gcgttttacc aaaaaaataa ttttgaaatc
     9841 tatggcgaac tcaaagacat gccgtttcgt ggcgttgata cgtattactt agtcaaacgt
     9901 ttgatggcat aacgtttaaa gttccaataa agaataaaga ggcacaaaaa tgacctacaa
     9961 agaaatacca gtctctgttc aacaatttat ggaaaaaatc acgactcttt gtggtaatga
    10021 ccacccacaa tgggcagcaa acattaatgc tgcctttgcc aatactcttt taacaacggt
    10081 taagcgacat gaagatggca caactttttt gttgactggc gatattcccg caatgtggtt
    10141 gcgggattca actgcccaag ttcggcctta tttagtcatc gccaaagata atccagattt
    10201 ggcggatatg attagtggtt tagtaaaaaa acaattcttc tatattaact tggatcctta
    10261 tgcaaatgct tttaatgaag aggccaacaa tgcggggcat caggatgacg aaacacaaat
    10321 gaatccttgg gtctgggaac gaaagtatga aattgattct ctttgctatc caatccagct
    10381 ggcttatttg ttatatcaga atactggccg tacagatcaa tttagtgaag actttattag
    10441 ggggatcgag aaggtcctga cggtttttga aacggaacag aaccatcaag actcacccta
    10501 cacttttcaa cggtttgatg atcgaccgga agatacgtta gtcaatcacg gacgggaaac
    10561 gacagttgca ccaacgggta tgacttggtc aggttttcgt ccaagtgatg atgcttgcca
    10621 ttatggttat cttgttccgt caaatatgtt tgcggtggtc atccttgatt atttagtaga
    10681 gattttccaa gatattttga aggagacgac actgattgac cgtattatca agttaaaaaa
    10741 gcgatcaatg atggtatcca aaaatttggt aagattttta ata
//
LOCUS       DACERL010000080        10771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.085, whole genome shotgun sequence.
ACCESSION   DACERL010000080 DACERL010000000
VERSION     DACERL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.085"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXF54_002356"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXF54_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1405585.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IXF54_002357"
     CDS             1995..4661
                     /locus_tag="IXF54_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1405586.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IXF54_002358"
     CDS             5025..5777
                     /locus_tag="IXF54_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1405587.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IXF54_002359"
     CDS             complement(5799..6953)
                     /locus_tag="IXF54_002359"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1405588.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IXF54_002360"
     CDS             7138..9579
                     /locus_tag="IXF54_002360"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1405589.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IXF54_002361"
     CDS             complement(9909..10613)
                     /locus_tag="IXF54_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405590.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3439 a         1932 c         2271 g         3129 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat g
//
LOCUS       DACERL010000081        10770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.086, whole genome shotgun sequence.
ACCESSION   DACERL010000081 DACERL010000000
VERSION     DACERL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.086"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            79..1125
                     /locus_tag="IXF54_002362"
     CDS             79..1125
                     /locus_tag="IXF54_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1405591.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IXF54_002363"
     CDS             1474..2106
                     /locus_tag="IXF54_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1405592.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IXF54_002364"
     CDS             2694..4133
                     /locus_tag="IXF54_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1405593.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IXF54_002365"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IXF54_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IXF54_002366"
     CDS             4895..5110
                     /locus_tag="IXF54_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405594.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IXF54_002367"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IXF54_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IXF54_002368"
     CDS             complement(5770..6681)
                     /locus_tag="IXF54_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1405595.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IXF54_002369"
     CDS             complement(6700..7704)
                     /locus_tag="IXF54_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1405596.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IXF54_002370"
     CDS             complement(7701..9824)
                     /locus_tag="IXF54_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1405597.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IXF54_002371"
     CDS             complement(9829..10749)
                     /locus_tag="IXF54_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1405598.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3294 a         2034 c         2019 g         3423 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg
//
LOCUS       DACERL010000082        10662 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.087, whole genome shotgun sequence.
ACCESSION   DACERL010000082 DACERL010000000
VERSION     DACERL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.087"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            127..579
                     /locus_tag="IXF54_002372"
     CDS             127..579
                     /locus_tag="IXF54_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405599.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IXF54_002373"
     CDS             593..1282
                     /locus_tag="IXF54_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405600.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IXF54_002374"
     CDS             1407..3758
                     /locus_tag="IXF54_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1405601.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IXF54_002375"
     CDS             3838..4233
                     /locus_tag="IXF54_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405602.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IXF54_002376"
     CDS             4558..5175
                     /locus_tag="IXF54_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1405603.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IXF54_002377"
     CDS             5216..5515
                     /locus_tag="IXF54_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405604.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IXF54_002378"
     CDS             5549..5716
                     /locus_tag="IXF54_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1405605.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="IXF54_002379"
     CDS             complement(5761..6366)
                     /locus_tag="IXF54_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1405606.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6491..9478
                     /locus_tag="IXF54_002380"
     CDS             6491..9478
                     /locus_tag="IXF54_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1405607.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9645..10034
                     /locus_tag="IXF54_002381"
                     /pseudo
     CDS             9645..10034
                     /locus_tag="IXF54_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            10168..10437
                     /locus_tag="IXF54_002382"
     CDS             10168..10437
                     /locus_tag="IXF54_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ1405608.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVQYLKILCIIGIEVKLDAKNL"
     gene            10458..10541
                     /locus_tag="IXF54_002383"
     CDS             10458..10541
                     /locus_tag="IXF54_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1405609.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         3723 a         1568 c         2026 g         3345 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggacttttg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt ttatttgcat gaaaaagatt tagaattgat
     6601 ggagactata cgacgaccag ctactagatt aggttttgca ctacagttag tctcactacg
     6661 ttttttagga acttttatat tagatcttac caatattccc caaaatgtat tgagttatgt
     6721 agcaactcaa ctaaatattg atcctggaga aattatctac tatagcagga gacagaccag
     6781 gtatcagcac acaaaattga taaaatcaca ttatggatat caagatttca accaaccgga
     6841 aatagaaaca tacctagcta actggctttt aaatagagcg ctgtatacaa cagaatcttc
     6901 agatatgttg tttgatatgc ttctaaaaaa atgtctggat gaaaagattg ttttacctgg
     6961 aattactact ttttctcgtt ttgttgctag tgcaattgaa aaagctgaag agcatttata
     7021 ccgacaattg tctttggttc caactgaaga tgagagaaag cggcttctca atcatttaga
     7081 attaattgga actccagttt atggggcaat aatcaaaatg gacattttga gaaccccgtt
     7141 agctgacgat agtcataaag aaatagccag aggttttgac cgactaaagc aatttcaagc
     7201 tttctctact gaaaaatgga agatggaatc tattcctgaa gggaaaataa aagttcttgc
     7261 aaattatgca tttaaagcta aagctcaatt aattcaacga atgagtgaac agaaaaaact
     7321 ggcgttgtta gtggcattta ttttcatata caaacgtaag gctatggatg aacaaatttt
     7381 agcactatct aatttttttg aaactgtttt tagacgcgcc aaaaataagg aatctaaaga
     7441 acgtcttcgt accataaaag atctagatcg agccgcatcg acactttcag atattgtaga
     7501 tttagtaatg tccagtcatt ctgatactaa tgaactatcc cttcgcgatc gtatcttaga
     7561 aaaatattct gaggaaaatg ttaccgaagc tgtatcgcaa gttcggctat tggttaaaaa
     7621 tgatcgtgaa ccaatagcca tttctgaact tctgaacact tatcgaaagt tcagaaaatt
     7681 tattccggat attttaacta cactaacatt tgacagcaat gactacggta acaactgtaa
     7741 ggaactctgg accttcatta ataatagatt tcctaagccg attacttaca aaatgtttgg
     7801 agctattaaa catactcttc caaagaaatg gatttattac attcatgaat accctgaaca
     7861 aactaataaa tgtgtattga ttgcagctgt cgaattgtta attcaatctt taaaacgcca
     7921 tgatatttat attcctcaga gtgtcttata taacaatcct atggattgct tgattgatga
     7981 gaaaacttgg gttcaacaaa aatacgtttt attagaacaa ctagatcttc cttcttcagc
     8041 aaatgaaatg tctttatggt taaagaacga tttggaactg tcttataaag aagcgctaaa
     8101 tcgttggcct acttctaaaa tggccagaat agaaaatgca gatcgcctgg ttgtctctag
     8161 acttaaaaag acatatgaaa aaaaggaaga taagtctttt agacgtcgca ttcagcagtt
     8221 acttcctaaa attgatttgt cagatttgtt attggaagta aaccaacagt taaatcttac
     8281 tcaatcattt aaccacttaa gtgacaagga caccaaaatg aaagacttgg atattagttt
     8341 attagcagtt cttttatcag agtcctgcaa tattggtttt tccccggttt caaaagaagg
     8401 atttgattct ttaaagtatg atcgtcttgt ctatgtcaat catcattata ttcgagtgga
     8461 tacactttca ggagctaatc aaaaaataat acatgcacac aggaaacttg ccacctctaa
     8521 gatatggggt gatgggcata tggcttcagc tgacggtatt cgttatatca caccacaacg
     8581 ttccctatat tctagaagta atccaaaata ttttggccgt ggtcgtggga tcacattcta
     8641 caattttgtc tctgatcaat atatagggtt ccacggaatg gtcgtttcag ggacactgcg
     8701 agattcgctt taccttctag aaggattcct caatcaaccc agcgaattaa ctcctaatca
     8761 gatcatgact gatacagctg gttatagcga tatgattttt ggtctctttg gcttattagg
     8821 gtttcaattt agtccaagaa ttgccaatga taaaggaacc aaactttggc gaatcgacgc
     8881 taacgctcac tacggggaac ttgatacgtt gtctcaaaat tcaattaata tggaactgat
     8941 ttatacccat tgggaggaca ttcttagggt tgcaggttcg ttgaaatctg gaaaagtgaa
     9001 tgcaacagaa ttaacgaaat ctcttcaacg gaatgggcaa cccacgcctt taggaaaagc
     9061 aataactgag tatggaaaag tttataagac gaaacatcag cttcgttata tttctgacga
     9121 aatatatgca cggcaaattt tagaacaatt gaataagggg gaagctcggc atgccttatg
     9181 ccgtaatatt ttttatggac gaaaaggaaa actctaccag acttattatg atgggatgga
     9241 agagcagctc aatgcattag gattagttac taatgccatc atatattgga actcactgta
     9301 tttagaaaaa gtatttgaac aaatgaaagc tgaaggattc gactgttccg aagaaatgat
     9361 acgcaggcaa tctccactaa ttagtgatca catcaatttt gttggtaagt atacgtttag
     9421 atacgaagct gaattatcca acggtaagtt acgtccattg aatgtagaag aattctagca
     9481 atgttcgggt tttgcatgct accgcactat ttcgtatgac tgttccagcg aatcacttcg
     9541 aatagaaaaa gagaaaaaca cgagaacagc gttaaatcaa cgtttcttgt gtttttactt
     9601 ttgtgctacc gcactatttc gtttctatgt tccatgtacc ccgtaatcaa gaaaacttta
     9661 ttaataatat caagaaattt atcgttcatt tattggaaaa tgtacctacc gatatagaaa
     9721 aattaaattt ttctgattat caggaacaga aagaaaaaga agcagaaaaa aatattgttg
     9781 gcaaatgccc gaaatgcgga aacaatatta tactgaaaaa atcgttttat ggttgttcaa
     9841 attatcctga atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa
     9901 cgaatgtaaa agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag
     9961 agaaaaagct ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatat
    10021 ctttctcaaa ataaacataa aagcccttta aacagggctt ttatatatta atcacttatc
    10081 acaaatcaca aatcacttat cacaaatcac ttgtgatttg tgattgttga tgataaaata
    10141 agaataagaa gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc acaaaaagaa
    10201 aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa gacggttcgt
    10261 gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg cggaaacgta
    10321 aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat agtgcagtat
    10381 cttaaaattt tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta
    10441 agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg
    10501 ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag attaattcct
    10561 accagtgact aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta
    10621 acttctgtat ttatttacag atgtaatcac ttcaggagtg at
//
LOCUS       DACERL010000083        10462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.088, whole genome shotgun sequence.
ACCESSION   DACERL010000083 DACERL010000000
VERSION     DACERL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.088"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(172..924)
                     /gene="xth"
                     /locus_tag="IXF54_002384"
     CDS             complement(172..924)
                     /gene="xth"
                     /locus_tag="IXF54_002384"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1405610.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1037..1957
                     /locus_tag="IXF54_002385"
     CDS             1037..1957
                     /locus_tag="IXF54_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1405611.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2030..2965
                     /gene="murB"
                     /locus_tag="IXF54_002386"
     CDS             2030..2965
                     /gene="murB"
                     /locus_tag="IXF54_002386"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1405612.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3263..4741
                     /locus_tag="IXF54_002387"
     CDS             3263..4741
                     /locus_tag="IXF54_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1405613.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5018..7078
                     /locus_tag="IXF54_002388"
     CDS             5018..7078
                     /locus_tag="IXF54_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405614.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7161..7730
                     /locus_tag="IXF54_002389"
     CDS             7161..7730
                     /locus_tag="IXF54_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405615.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7905..8711)
                     /locus_tag="IXF54_002390"
     CDS             complement(7905..8711)
                     /locus_tag="IXF54_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405616.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8849..10363
                     /locus_tag="IXF54_002391"
     CDS             8849..10363
                     /locus_tag="IXF54_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1405617.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3706 a         1743 c         2063 g         2950 t
ORIGIN      
        1 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaatctct cgataaacag
       61 tgttcaaaag ccagtttctc ggtattatgc caaatttatg caaaatgtgg aaagccattt
      121 tcacaaaatt tttcttaata ctcgaaacta aacgactttt tcacaacctc tttatttcaa
      181 atctaattct actggacaat gatcactgcc cataatgtcc gtatggatct ttgcatcagc
      241 tagtcctggc tctaaatctt tgctcgtcaa aaagtaatcg atccgccaac cagcattgtt
      301 tttccttgaa ttgaatcggt agttccacca agagtagaca ccttcctggg ttggataaaa
      361 ataacggaat gtatcgataa acccgttatc caacagtcga gaaagagccg ctctttcttc
      421 tggagtgaat ccagcatttt tttgattcgt tttccagttt ttcaaatcaa tcttttgatg
      481 agcgacattt agatccccac acacgatgac tggtttttgt ttttttagat tttccaaata
      541 attataaaag gcttcttccc attctaatcg gtaatctaaa cgttttaatt cgctttgaga
      601 atttggcgtg taacaggtta caagaaagaa atcggaatat tccaaagtaa tcagacggcc
      661 ttctgtatca tggatatcga tacccattcc gtaagtggca ttcaatgctg gttttttagc
      721 aaaaatcgct gtgccggaat agccttttcg ttccgcgtaa ttccaatatt gataataacc
      781 tggaagatct aaatcaattt gtcctgcttg gagtttcgtt tcctgcagac agaaaaaatc
      841 ggcgtctaac tcatgaaaaa cttctaaaaa atttttattt acgatggcac gaagcccgtt
      901 tacgttccaa gagataaatt tcaataaaag cacactcctt cgaattaatt tccttacctt
      961 tgcgatacta ttgtacctca ttttagtaaa aaatggtgta agatgaaagt aactaaatga
     1021 tccaggagga aagtagatga agattggtgt aatcggtgta gggaatatcg cagaaaaagc
     1081 atatttacca acttatgcga aaaaacaagg aacacttgat ttttattttg caacaagaaa
     1141 taaagaaaca aaagaaagaa ttcaaaaaga gtacgggttc caacatctat atgaaacgat
     1201 cgatgagctt ttagaagaaa aaatcgaagc atgtatgatc cacgcagcaa caaaagtaca
     1261 ttatgaatta gccaaaaaat gtctagaaaa tggtatccat gtttatattg acaaaccact
     1321 gagtgtccat ctaaatgaag ttcgtgaatt gcaagaatta gctgataaga accaaataat
     1381 tttgatgatc ggtttcaacc ggcgctttgc tcctatggta gaagaattga aacgtatccc
     1441 agataagcgg ttgatccacc tgcaaaaaaa tcgaatagcg gcaaaagaaa cgacagagtt
     1501 cgtcctttat gatttgtttt tacatcttat tgatacagct gtgtatttat tagatgaacc
     1561 cattttgaga acaaaatacc agataagaga aacaaaggaa tttttggaat atgccatttt
     1621 acaattagaa acacaaaatc aaacggctat tttaacgatg gatttagcaa gtggcgctaa
     1681 tatagaaaaa tatcaagtaa caagtaaaaa tggtacttat gaagtaaacg atctgaccca
     1741 gcttgtcatt caagatgctg atggaaaaaa agtgaaagaa tttggcgact ggacaaacac
     1801 gctagtcaaa cgcgggtttg agccaatggt agaggccttc ttttctcaga tccgtttagg
     1861 taaatcggaa acagaattgc ttaaacaaaa agaagttgta agaagtcatg aattatgtga
     1921 ggaagtgctt aaagatcact accgtcatca actttgaaca agactggcaa gattgtatta
     1981 agcgtgatag aataatgagg acgtttgaga aaagaaagga aaatcctcag tgaataaaga
     2041 agaaatagtt gaaagcttag ccacgattac tatattgttt gatgaaccat tgatgaacta
     2101 tacgtttacg aaaacaggtg gaccagctga tgtccttgca ttcccgaaaa aacaagaaga
     2161 agtaaagcaa atcatcgatt attgccgaat ccatgatata ccttggatgg tattaggaaa
     2221 tgcaagcaat ctgatcgttc aagatggcgg gattcgcggt gtagtcatca tgctgactga
     2281 aatgaaacag attcatgtca aaggaacgat ggtcattgcg gaagcaggtg cctcactgat
     2341 tgatacgact tatgcagctt tggcagaatc attgactgga tttgaatttg cttgcggcat
     2401 cccgggaagt gtcggtggag cggtttatat gaatgcaggt gcgtatggcg gagagatcaa
     2461 agatgttttt gctgaagtgg atcttttgct tgaagatgga acattgaaaa ctttgacaaa
     2521 agacgaaatg gtattttctt atcgtcacag taaagtccaa gaattacgtg ctatcgtatt
     2581 ggaagctcga ttcagtttgc aaacagggga ttatgaagca atcaaagcaa gaatggatga
     2641 gttgaccgaa ttgcgtcaat caaaacagcc attagaatac ccttcatgcg gaagtgtctt
     2701 taagcgccca gtaggacact atacaggtca gctgatccaa caagcaggat tgcaaggcct
     2761 gaaatggggc ggtgcacaag tctctgaaaa acatgctgga ttcatcgtca atatcgatca
     2821 tgcgacagcg acagattata ttgaattgat tgcgcacatc caagaagtaa tcaaagaaaa
     2881 atttgatgtg tctttagaaa cagaagtgcg gatcattgga gaaaaacaag caatatcaga
     2941 aacaagcaga agtttagcca attaataagt aatggaactt aaaataagta aaagagcagg
     3001 acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa tcttgtacat
     3061 tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc attttttgca
     3121 atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag cataatccat
     3181 cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta attggtatga
     3241 ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg aagatagaga
     3301 aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag tgctgcctaa
     3361 gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca tagaattaaa
     3421 taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag aagcagagat
     3481 ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct actttcaacg
     3541 atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga tcgaaaaatt
     3601 tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag aattagtctc
     3661 ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg aagaaataaa
     3721 catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt ctatgccatt
     3781 tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat atctgttcaa
     3841 tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta ttagtttgtt
     3901 gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat actttaagag
     3961 ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc atatccctaa
     4021 agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg aagaagaatt
     4081 tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc acatgattag
     4141 tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa tctctgaaga
     4201 agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa
     4261 ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac agtttttctt
     4321 cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga ataaacgcta
     4381 tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc tctttttatt
     4441 ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta ttgatttttc
     4501 gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta aaaattacaa
     4561 tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg attgtttact
     4621 tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag atgactggga
     4681 atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa aaatcagcta
     4741 gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt ttttttcaaa
     4801 gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag
     4861 tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct
     4921 aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga aaattactac
     4981 ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa
     5041 acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta
     5101 cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt
     5161 acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac
     5221 tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc
     5281 tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat
     5341 tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc
     5401 cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca
     5461 tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga
     5521 ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc
     5581 tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat
     5641 ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc
     5701 ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc
     5761 tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc
     5821 taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat
     5881 caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta
     5941 tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc
     6001 tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca
     6061 agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt
     6121 ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc
     6181 tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct
     6241 tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt
     6301 tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc
     6361 aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc
     6421 cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt
     6481 gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca
     6541 agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa
     6601 agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt
     6661 ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa
     6721 aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg
     6781 attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc
     6841 taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt
     6901 gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg
     6961 aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat
     7021 ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt
     7081 tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg
     7141 agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga
     7201 caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat
     7261 tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc
     7321 aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac
     7381 ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg
     7441 cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaac
     7501 cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc
     7561 ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga
     7621 ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta
     7681 atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg
     7741 aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt
     7801 tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg
     7861 acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt
     7921 ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa
     7981 ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc
     8041 atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca
     8101 ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct
     8161 ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc
     8221 agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa
     8281 cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt atttttattg
     8341 agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga
     8401 aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa
     8461 ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc
     8521 ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt
     8581 ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa
     8641 atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc
     8701 aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt
     8761 taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat
     8821 atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc
     8881 cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag
     8941 ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga
     9001 tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat
     9061 ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc
     9121 tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt
     9181 tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg
     9241 tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc
     9301 ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat
     9361 gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga
     9421 ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac
     9481 agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga
     9541 accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa
     9601 agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg
     9661 atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga
     9721 taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt
     9781 tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga
     9841 tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat
     9901 ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata
     9961 tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac
    10021 actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt
    10081 caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat
    10141 cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt
    10201 agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt
    10261 acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca
    10321 agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa
    10381 aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt
    10441 tagtgaagag gttgtgaaaa ag
//
LOCUS       DACERL010000084         9967 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.089, whole genome shotgun sequence.
ACCESSION   DACERL010000084 DACERL010000000
VERSION     DACERL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.089"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(61..1572)
                     /locus_tag="IXF54_002392"
     CDS             complement(61..1572)
                     /locus_tag="IXF54_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1405618.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1894..3246)
                     /locus_tag="IXF54_002393"
     CDS             complement(1894..3246)
                     /locus_tag="IXF54_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1405619.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKDSLSMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3399..4475)
                     /locus_tag="IXF54_002394"
     CDS             complement(3399..4475)
                     /locus_tag="IXF54_002394"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1405620.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4671..6416
                     /locus_tag="IXF54_002395"
     CDS             4671..6416
                     /locus_tag="IXF54_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1405621.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTLGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6665..7132)
                     /locus_tag="IXF54_002396"
     CDS             complement(6665..7132)
                     /locus_tag="IXF54_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1405622.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(7116..8489)
                     /locus_tag="IXF54_002397"
     CDS             complement(7116..8489)
                     /locus_tag="IXF54_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405623.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKEIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNHESVSIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8812..9300)
                     /locus_tag="IXF54_002398"
     CDS             complement(8812..9300)
                     /locus_tag="IXF54_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405624.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELVSDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9322..9924)
                     /locus_tag="IXF54_002399"
     CDS             complement(9322..9924)
                     /locus_tag="IXF54_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1405625.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2918 a         2120 c         1797 g         3132 t
ORIGIN      
        1 aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt
       61 ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc
      121 atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt
      181 accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc
      241 ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg
      301 atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa
      361 taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat
      421 ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa
      481 agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg
      541 tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg
      601 aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat
      661 atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa
      721 tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg
      781 gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa
      841 gacgattgtt agttcaggtg tttgaacatc aaacaagcga gtcatgatat cgaacttttc
      901 gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt
      961 gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg
     1021 catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc
     1081 gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg
     1141 aacggttcca agtttcaatg tgtggcggtt gatgtggtca agcatacgtc ctggtgtacc
     1201 cacaacgata tgtggacgat ctttcaagcc acggatctgt cggccaatat ctgctccgcc
     1261 gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt
     1321 ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg
     1381 atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtttgtgc
     1441 ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgatt ggataggcgt
     1501 tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc
     1561 tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta
     1621 ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt
     1681 ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta
     1741 gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt
     1801 tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac
     1861 atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa gctgttctac
     1921 cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag
     1981 attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag
     2041 ttgggggtat ttttgattca attcttcttt taatgcatgc atttgttccc cgtacaagaa
     2101 aatcatggca aatttctcag gatcgatatg ttcactcatt tgcgcatgca tgcttaaaga
     2161 atctttccct aattccagca tatctgctaa cacagcaatt ctgcgccctt ttgttggcag
     2221 atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattat agacatcgct
     2281 caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac
     2341 atgagcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata
     2401 cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc
     2461 ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc
     2521 atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa
     2581 cggttcatct gcgggcacaa cgagtaatcc gtctggtaca agtccgtcag tgatttccat
     2641 tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat
     2701 tgtgattgct gcagcttttg gttgagccat cagtgaaagt tccgtgattt cattggcgtg
     2761 atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta
     2821 aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga
     2881 caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac
     2941 gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg
     3001 atctgctaca ggaataactg gaaaatcttt tggagcttgt tcaacaggca agctccaaaa
     3061 tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc
     3121 agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt
     3181 aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag
     3241 attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt
     3301 tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg
     3361 cggttacgca atcactccag cttcttcttt atttctcttt attcattttt ttcaaaaaaa
     3421 gattgacgct gatggtatcg attcattcct aattgaatca gttcttcaat caaatcaccg
     3481 tatttcaagc ccatattttc ccataagagt gggtacatac tgaactctgt aaatcctggc
     3541 atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa atcacaccgg
     3601 ctcaatccgc ttccgcctaa catcgtgtaa gctaacttcg cgtactcttg tgctttttga
     3661 taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt
     3721 gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg
     3781 tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt
     3841 gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct
     3901 gcctttgtaa tgccgacact agaacccata ttcgcaggtt tgacaaacat cggataaagc
     3961 aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt
     4021 actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt
     4081 ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc
     4141 tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg
     4201 atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct
     4261 gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt
     4321 agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa
     4381 taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct
     4441 gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat
     4501 cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta
     4561 taaaaaaacg agttggtttc taaaagaaca aacgtcattt attttttgtc agctagatta
     4621 tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa
     4681 caaatccacg tagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc
     4741 caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc
     4801 ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct
     4861 tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca
     4921 aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa
     4981 aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg
     5041 actcggtcgt cgttaagatc caacacccta acatcgccga agagattcga cttgatcttc
     5101 aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc
     5161 taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag
     5221 aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc
     5281 ccaaaattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta
     5341 aaaacttgaa tcatttgatg aatgccgaaa acggaacaga aacttttatc accggtattc
     5401 aaaacaaaca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg
     5461 tatttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac
     5521 taacaaaaga agaacagaca cgagctagcc aaaaaactga aacagtccat gaaaaagagt
     5581 tcggttcctt tacttttcga gcctctactt ctacagaaga ccctgttgcg ccatataccg
     5641 tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca
     5701 cacaagccgt tttagcgctg tatacaaaag atgcttatcg aatcgaaaaa gcggtccttc
     5761 gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt
     5821 ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac
     5881 acgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc
     5941 tgaaagcatt tgggacatta gaaggggtga tccgcgtcct tgaccctgaa gtttccctca
     6001 tggaagtcgc ttcgcctttt gcccaacatt attttctcac tcatctggat gtagaagata
     6061 cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc
     6121 aacagctcca ccatctatta gaaatgtgga ctttgggaca aggaaaagtc aatttggaat
     6181 tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg
     6241 gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag
     6301 aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca
     6361 ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa
     6421 aggttgtgac agaaagaatc agcttcaaga aataagaagg gattttgcat aaattgtttc
     6481 tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa gcaccgagaa
     6541 taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa aggaacatgg
     6601 aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc
     6661 ttcgttacct actatcttct gtacttggta aaccttggtc aattgccttt tttaacgctt
     6721 gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag
     6781 cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt
     6841 caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta
     6901 aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat
     6961 ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg
     7021 tcgtataatg gtccacactg gttacgataa tcacgtcgag accataggat tctcctaatt
     7081 ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc
     7141 gccttcttcc ataaatgata aacagttact aatccgctca gtaagagacc aacattgatc
     7201 cattttgcca agataagcag ttgtacactt tctatcgaca ctgattcatg gttcagcgtg
     7261 atcaataata gatggattgg ctctcccatt tcttttgcta aaataaacaa aagcgtcatc
     7321 aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg
     7381 cgatccattg gaatagctgc atcagtttga taatattcct tatttagcca gctgcctgtt
     7441 actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg
     7501 accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca tgataaacgt
     7561 ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac
     7621 ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaacaaa
     7681 aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac
     7741 ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa
     7801 ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga
     7861 aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt
     7921 ttgatctctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccata
     7981 acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca
     8041 atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata
     8101 ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat
     8161 gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct
     8221 aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa
     8281 ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa
     8341 aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa
     8401 agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta
     8461 cgcccaaatc gtttgagttt ttcgaacatg acaatggctc ccttaaacag tcttataact
     8521 aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct
     8581 tgtaaaaaaa aatgcagaaa gaagcataag cttctttctg catttttacg gtacttgatt
     8641 actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg
     8701 tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc
     8761 agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag
     8821 agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg aaacaagttc
     8881 ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat ttccctggtt
     8941 cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa
     9001 aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata
     9061 aatttttcca tttatctctt cataagcagt gtaaccgacc gtcttttctt cgctttttaa
     9121 taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt
     9181 tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaagctt ccggccatac
     9241 ttcttgtatg actgccatca gctcatccaa ttcattttct gttactttta ttttttccat
     9301 acacccacgt ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat
     9361 gatcatgcta tacacaccta ccgaccatgg tttcaagaaa atcacaatct gcataaattc
     9421 cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg
     9481 aagcagcaag gtaacgacta gtactttttt gatatctttc gtttttcttg tcagcaattc
     9541 atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta
     9601 acggacaagt aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag
     9661 cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat
     9721 aggaatgatc acttggatca cttgcagcag aacaaatatc atcactgcat acttgccaaa
     9781 ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata
     9841 aagaatcaac acaaagaaaa acaacagacc aataatgggc atgatctgaa gacatttttg
     9901 taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact
     9961 gcctcct
//
LOCUS       DACERL010000085         9665 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.090, whole genome shotgun sequence.
ACCESSION   DACERL010000085 DACERL010000000
VERSION     DACERL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.090"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..650)
                     /locus_tag="IXF54_002400"
     CDS             complement(<1..650)
                     /locus_tag="IXF54_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1405626.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IXF54_002401"
     CDS             975..1391
                     /locus_tag="IXF54_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1405627.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IXF54_002402"
     CDS             1388..1858
                     /locus_tag="IXF54_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405628.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IXF54_002403"
     CDS             1872..2645
                     /locus_tag="IXF54_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405629.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IXF54_002404"
     CDS             2659..3471
                     /locus_tag="IXF54_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1405630.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IXF54_002405"
     CDS             3525..4490
                     /locus_tag="IXF54_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1405631.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IXF54_002406"
     CDS             4606..7329
                     /locus_tag="IXF54_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1405632.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IXF54_002407"
     CDS             7342..7974
                     /locus_tag="IXF54_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1405633.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="IXF54_002408"
     CDS             8873..>9665
                     /locus_tag="IXF54_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1405634.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1476 c         1885 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaactgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DACERL010000086         9607 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.091, whole genome shotgun sequence.
ACCESSION   DACERL010000086 DACERL010000000
VERSION     DACERL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.091"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(293..1135)
                     /locus_tag="IXF54_002409"
     CDS             complement(293..1135)
                     /locus_tag="IXF54_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1405635.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1172..2638)
                     /locus_tag="IXF54_002410"
     CDS             complement(1172..2638)
                     /locus_tag="IXF54_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1405636.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2694..4538)
                     /locus_tag="IXF54_002411"
     CDS             complement(2694..4538)
                     /locus_tag="IXF54_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1405637.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            5140..6090
                     /locus_tag="IXF54_002412"
     CDS             5140..6090
                     /locus_tag="IXF54_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1405638.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(6135..6768)
                     /locus_tag="IXF54_002413"
                     /pseudo
     CDS             complement(6135..6768)
                     /locus_tag="IXF54_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(7028..7576)
                     /locus_tag="IXF54_002414"
     CDS             complement(7028..7576)
                     /locus_tag="IXF54_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405639.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7577..8434)
                     /locus_tag="IXF54_002415"
     CDS             complement(7577..8434)
                     /locus_tag="IXF54_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1405640.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8720..9007)
                     /locus_tag="IXF54_002416"
     CDS             complement(8720..9007)
                     /locus_tag="IXF54_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1405641.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8997..9326)
                     /locus_tag="IXF54_002417"
     CDS             complement(8997..9326)
                     /locus_tag="IXF54_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1405642.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9403..9549)
                     /locus_tag="IXF54_002418"
                     /pseudo
     CDS             complement(9403..9549)
                     /locus_tag="IXF54_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2901 a         1940 c         1464 g         3302 t
ORIGIN      
        1 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
       61 atttttaacc aaataaacag aacagaaaga ggttttcttc tttctgttct gtccaaatta
      121 ttctaagatt tctctttacc aacatcattt gacaaagagc cgagattttt tattatctta
      181 cgttaatcct ttttaaatgt ctcttataga taacaaacag ctggaaatca ccttgttttt
      241 actataatcc atctctaaag attttaaaag catcaatagt attactgagc gctcacccat
      301 tagcatgttt catcaaccga ttgatgtgga cacctaggta tacaacttca tcgttcggaa
      361 ttgttttatg ataaactttt tcaatgtagt ctttgatctt agctgcgctt tccatggcag
      421 acgggtataa gatccacatt tgattgtaga attcttcctc accatcatcc aacaaagcat
      481 cagaataata tctttctaca aagaaacgta catgtgtaat gaagcgggca taatggatcg
      541 aattagtatc gatatctttc tgcaacgaat accggaccaa attgacgatc ccgccgatca
      601 tttttgcata cttgaatcca gcgccgctat caggatcttc cgattgtgca ttgatcaaat
      661 ggaaagcaat gtttgaggct tcctctttag gaagatcaat tccatattgc tcgttcaaaa
      721 gggtgagtgc gtacttccca atttgaaatt ctttgggata gtagattttt atttcccagt
      781 acaaacggtt tgcaatattt accccttttt gtgcgcgttc aactgcaaaa tgcaggtggt
      841 ctgttaaggt taaatagact ccaggattca gttttttatc taatcctttt tctgcttcaa
      901 aaacgatagt acgagtgatt tcaaaaaatt caataggtat ttcttcaact aactctaata
      961 gttgtgaaga tttcttttct tcaaaaggca gaaatatttt atctacttta tctcctgata
     1021 tttcatcccc agctttctgt ccaaagccta tccctttacc taatgcaatc atttctttgc
     1081 cattttcttc aaccagtacc acacttgaat tcaatacttt ttttattgaa atcactcatt
     1141 atccccctat cgctgcttaa aaagatcgag actactcgaa taaggatgca ccgtttgttt
     1201 cgatcacttc tttgtaccag ttgaacgaat cctttttcaa gcggttatat gtcccatttc
     1261 cttcatcgtc tgcatccaca taaataaatc catatcgttt agacatttga gaagttcctg
     1321 cactaactaa atcaattggt ccccagctag tataagctat caaatctaca ccttcatcga
     1381 ttgcttcttt catttgttgg aagtgtgctt taaagtagtc gatgcggtac ggatcatgga
     1441 ttttaccatc tactacctga tcgactgctc ccataccatt ttccacaatc atcaacggta
     1501 cttggtaacg atcataaagt tcgatcaacg aaattttcaa accttttgga tcgatttgcc
     1561 agccccaatc tgttgaaggc aaatgaggat tcttaacccc taaaacagta tttcccggtg
     1621 tgcgttctgc gtttggatca gctgattctg ccatggacat gtaatagctg aaagaaacaa
     1681 aatctacggt atgtttcttt aaaatttctc gatcttcatc ttccatttta atctgaattc
     1741 cccgattttc caaatccttt tcgattagag caggatattc tcccttaact tgaacatctg
     1801 cataaaaatt attttctaag ttctttttca aggtcagctc aacgtcttct ggtgcgcaag
     1861 ttaatggata agtcatcaat ttcgttaaca tacaaccaac ttggcttcct ggaatttttt
     1921 catgagcatc ttttgtcact aacgcactcg caacaaattg atgatgcaat gcttgataga
     1981 tatcttgagc ttcttgacct tctgcgcttt tctcttggat aattccagct gttgtaaatg
     2041 gatgacggtg gatgctgtct atctcattaa atgtcagcca atattttacg tatttgccga
     2101 agcgatcaaa acaaacgtta gcgaagcgaa caaaatcatc aattactcga cgctccaccc
     2161 agccattata tttaactgct aaagctaatg gcatttcata atgagatagt gtcacaatcg
     2221 gctcaattcc taaacgttgc atttctttaa agacgttttc ataaaatgca accccttttt
     2281 cattcggttc cagctcttct cctgtaggga acaagcgagt ccaagcaata gagaggcgca
     2341 aggttttaaa gcccatttcg gcaaataaag ctaaatcttc tttatagtga tgataaaaat
     2401 caacgcccct tctttttggg taccatttat cagatttgtc ttcactagct tcttttatag
     2461 actgcagggt gacatgcata tgagcagcaa aatctttgtt gtctaaattt ggtttataag
     2521 tagccatatc cgctactgaa agacctttac cgtctacatt ccaagcacct tctacttgat
     2581 ttgctgctat cgcgccgccc cataaaaatc cttttggaaa acttgttgtg tttgtcatta
     2641 ttctattctc ctctattcct tcattcaata tatttttttt cactatcttg tgtttaagct
     2701 atcggactga tagctaagag atcactatca ttggttacca atttttctag gttaaatgaa
     2761 cggatagtgt aattatcctt attggttacc acacagataa ctgtggggtc gaatcctgct
     2821 tctttgatta aatcaatatc aaatgcaatc agcttttgtc ctttggctac tttttcacca
     2881 gaacgaacaa atgcctcaaa cccttttcct tctaactgaa cagtatcaat accaatatga
     2941 aaaagaagtt ctgcaccatt agccgttttt aaaccaatag catgcttcgt ttcaaatacc
     3001 attgtgattt ctccatcttc aggagctact aatatccctt cttcaggaat gattgctact
     3061 ccatcaccta ccataccact agaaaaaaca gtgtctttta cttctgatag tgagattact
     3121 tttccattca cgggacttga aagaacagca tctgaaacaa cggccgtttc catcttagtt
     3181 tcaacttcat tgacaacttc tttatcttta taaagaacca acgctgcagc aaaagcaacc
     3241 acaaatgaaa tccccgatac tgcaaatgcc caaaccagat tcattgggtc agctttgtca
     3301 gcgaacatcg tgatacttgc aatcccagga cccactaaag caaaggcttg aagtgcaacg
     3361 attcctgcaa atgctccccc gatcaggcta gaaaacataa cggcatataa aacacgtttg
     3421 tgcagaatcg ttaccccgta aagagctggt tcagtaattc cgaataatgc agatataccc
     3481 gcagaaacag ccgtagattt caattttttg tcttttgttt tgatactgac agcaaagcat
     3541 gcgccagctt ctgaaatatt gtgtgccaat gaagccggga gataaagaag ttctgcaccc
     3601 aattcactca tggatgaaac agcgtaaggg atcattgctt tatgcatacc cgttacaacc
     3661 attaatggca gtacagctgc taagactccg gttgcaaccc atccaaattg attgtacaaa
     3721 gacacaatga tagaagagaa agctgttcct acattataac ctaatggtcc tagaagcaac
     3781 aaagcaatag gaactgtaat caataagctc atcataggta caaagaaaat acgaattggt
     3841 tttggtgaaa cttttgtaaa ccatttttca agttgagcat aaaataaaac agttaagatg
     3901 gctgggaata cttgagatgc atacgctata ttatccaatc caaaagataa gaatgtagca
     3961 ccttccgcca acatagctgt catttcagga aggatcaatg caccgacagc agatactgca
     4021 accaattgat tgactttcaa tttattagcc gtagtcattg ccaccagaat aggcaagaaa
     4081 taaagcggtg ccccgccgac caaatttaaa atctgatagg tttgagattg gtcactaatc
     4141 agccctaaga tagaagctag tagcaacaag gacttcaata ctcctccacc tgcaatcgca
     4201 ggaattaaag gttggaaaat agaaataata aaatctaata gaactgaacc taatttttgt
     4261 ttttgttttg gtgcttcttc agtatttgaa acattgtcac ctaataacgc tttcacttca
     4321 tcatacactt caacaacatc gtttccaatg attacttggc attgcacatt ttggcgaacg
     4381 cccataacgc catccacttt ttctaactgg ctcatattta ccttgctgtt gtcttttaac
     4441 gtaaaacgta aacgagttga gcaatgctcc aaatgggata tgttttcttc cccaccgatg
     4501 tactgcaata cttcttctgc tgtttgtttg taattcatgg tgtttccctc ttttcttttt
     4561 tgagttatga acttttgagt cgaagtggcc atcctcaaca caaaaaacag gcagaaacta
     4621 aataaattga caacaaggca aacgtgtatt gaaacgcata cattgttatt caacttattt
     4681 aggtcctgcc tgcattacca gtcacatgcc tgtttaagtt atattcaatt taacatatta
     4741 gaatgtaaac gtaaataata aaatgatttt ttaatcattt tttcatttta ttcgttcaat
     4801 atagttttcg tgttctaaaa gttttgtctt ttttctaaga atatcttcca acatatataa
     4861 ctcttattct cttctatctt ctagcagatg ttctgtcttt ttaagaccta gtataaaaag
     4921 aatgggttca ttttttcttt cttgcaggat gaggtttttt atataaactt tatttgatat
     4981 aaagaaaagg cattcataat tgtgctatac tactaaaaaa tgataataca gtgaattaga
     5041 ttagcgcttt attaaattaa gaggacagca tatcgatatt gcttcctttt ttttgctatt
     5101 tatttaaggg atagaagttt agaaaggact gtttataaga tgaaaaatga tatagtgact
     5161 tatgcaaaca caaaatgtga agaaattggc atcagtaatt tagaagagtg cttttttgac
     5221 acaaatgacc tgtcgcaatt tttaagaacg ggaagaacaa atgccagtaa acttgccaat
     5281 gacttagttg caaagaacgt tctatttaaa atcaatactc gtccagtgtt atttgccatc
     5341 aaagagcgct ttgaagagca ctttttgatt ccggtaaaag atgtatacga aagtatatct
     5401 gaattaaaaa cagacttgga gcccaattct acagattcca ttctacaaaa gatgattggg
     5461 tatgatatga gcttatccga tgcggtggaa caaatgaaaa ctgctgtctt ctatcctgga
     5521 agagggcttc ctatgatgat cactggagat actggagttg gaaaaagttt ttttgttcat
     5581 actgcttatc agtttatgaa aaaagtgaac gttattcatg agaatgctcc ttttaaagta
     5641 cttaattgtg cgcaatacca taataatcct gaattattgt cggggatact atttggctat
     5701 gttaaaggtg cttttaccgg cgcagatgaa gatcgaaatg gattgttgca agatgcagac
     5761 ggtggagtac tgtttttaga cgaagtacac agattgcctc ctgaagggca agagaaacta
     5821 tttgtttata tggatggcgg aacttattct agagtcggtg aaactatcca tcacgcctcc
     5881 aatgtacgtt tagtttttgc gactacagaa ggcaaagagg attttctgga aacctttatg
     5941 cgaagaatcc ctatacatat ccatatccca aatattgagg aacgaggtat tttagaaaaa
     6001 aaacaaatta tcgaaaatac tctctttagc gaaagcaaaa actttagcaa ggctattgat
     6061 gtttctccta aagtcactag tgttgcataa atatagtcag aatattctga attattattt
     6121 tcttgaaaaa agtaaaaaaa atcgtatccc aaactttcat ctacatttcc agaaagatct
     6181 tcatgttgat aatcaattcc tgaatcaatg accgctacct tcacatcctt agaacccttg
     6241 gtttcttccc aagcggatgg catgctaatc tctcttaaat tagcttgttt actccaatta
     6301 ggatcgttat cgatttccta aagaatcagt tgtatttggc tcctcaacat cattcaacgg
     6361 ctctagttca accacatagt ttggctctgc ataattgacc atatcagaag cattaaatgt
     6421 tcttactgcc tcttccacac taatttcttc gttcaatttc actaataata cttgatctcg
     6481 cttattataa tcgctttcat ttgtcctaag agtcaaatct ttttccacca taacttttac
     6541 acccaataaa ttttgcggtt tatttttctc tgttgaaatt ctttgtgtag cctttaaact
     6601 gacaattagc tcattgggta catactctat ttgagagtca tctaattggc tcataatcct
     6661 ttctttttgg ttagtatctg cagttacttg tttagtatga gccatgaacc caaacatact
     6721 caccccaaca accaatccta aatatatttt ctttttaaac tttttcatct acatctcctc
     6781 ctaatagttt tattttagag ttagctctat tacttattta ctaactcaac aacagattat
     6841 catacactta ttaatgaaat cacaatatct cattaaaaca caccctaaaa tcataaccgt
     6901 tcctatgctc attttttgga ttttttaatc acgtaaccag aaaaataaat cccctctatt
     6961 tcacgcaaat caaagaagaa ctaagtaact accgtttaaa ggtagttact tagttcttct
     7021 ttgattatta tctaacttta ttattcattc cccacccttt gcgcagtgag tcttcggtat
     7081 cttgttttat ctgactggta gttcttatta tttctccacg atcatctaat tctggaacaa
     7141 ttggatattt ttctctgaac aagtctagtt ctttcaccac gtcattaaac aaatcttgtt
     7201 tatcttcttg cgtaagattc tgtttaatat actcatctaa agcttttcta agcaattcat
     7261 ctcttgtcat attagtctca tacttttcta ctaaaactct ggaagtcgca tttaacaaag
     7321 tcgacagttt taaaggatgc ttggtggaat aaaccttatc aaactttctg attggtgcgc
     7381 caactttttt tgttcttcct ctactctgtc tttcaacatt ggaggaagtt tttatttctt
     7441 gaacttggtg cacttctaat tgcttatttt cagatggtgt aattgcttgc tgctttttct
     7501 ttgcttctgc ttcttttttt attttggcga gtcctttgtt gttcccaaaa atatcaagtc
     7561 ctttgatgtc agccaattac tccacctccg cataccaatt ctcgtgttct tccaattctt
     7621 tcaaaatact gataaataat tcgtggcttt tgcgatccca ttgttttaag agacctttct
     7681 ttgtcttctc catcgtaata cctgatccgt cgtatcgttt taagcgcgca tttttgagta
     7741 caacattttt aatcaaatgt tccccataaa tctcttgggc ttgtttatac atttcctgat
     7801 ctactgcgtc accgctttcc aacatgaaag gaataacacc agcaacatat aaatttgtat
     7861 caaattcttt cacaaaaaag tccatatact caagatattg catcgcattt ctcatcgatt
     7921 ttacttgggt ctgtaagacg acgattgccc aatccgcaat gtacatcgcg gatttactat
     7981 atacagaaat agaaggtggg acatctaaat agatgacgtc aaactcatca aataatggtt
     8041 ctagcttttc tttcatatat ttgaattggg ataatgaatc attatattgg tctttgatcc
     8101 aatcatcata catttcaaag gacatgtctg ctggaagtat atagaggtta ttgattactt
     8161 tatagacagc ctctttaggt ggaagattgt ttatcaacac gtcagtcaca gtattttcgt
     8221 aaacaatcgg ttcaccttct ccgcctcctt cttcgttagt gaagttcata tgcgttaagt
     8281 tcatcacatc agaagtattt ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt
     8341 cattaactgc tgattcaaac cctaacattt gcgcgcacgt acttttacct acgccacctt
     8401 taaaattacc gattacatat ttcttcgcta ccacgttcct acctcttttc ttttagcaat
     8461 attcatttga tactattata acatatttat ttttagttac taccaaaaaa aagatatata
     8521 tgtactacct tataattaat aagtaaaagg tagtacataa ataaggaaaa gctgaaatat
     8581 attgatttat agctttcctc aaagtaatga cgttatttaa tttatcgaga agcagagaat
     8641 actcgcgact ttagtcatgt agacgaatca gattttcgat aaaaaagaag ccgtccttct
     8701 taagaacgac aacaaatcac tagattaatt ttttcaactg ttcataaaag ttttcatgcg
     8761 ttccacataa caatactgga ataaccttgt tttctataat ctcgtaagct actctatatg
     8821 ttgtgcccgc atatttgaac ccactagacc aaatacctga taaatcccca gttttttgtt
     8881 cacccttata tggatcaatc gcaatttcat cataaattaa attcaaaaat ttttctttca
     8941 atggcttctc ttttagcttt tttaaatact taatgacatg ttttgaagga acgatttcat
     9001 aagccaattt ctgttgccaa ctcctcttta gtcattactt tagaattagt aagtgtatct
     9061 tcagcaatat ctctaaagga agaatgcatt ttgttttttc tattcttaaa ttctacaaga
     9121 atctcatctt tatcgtagcc ttctgcaatt atatcagaca aaatatcttc gctaaaatca
     9181 taaaattctt tttgtggttc aacaaattca taaagaatac cattctctac caactttgct
     9241 tcaacttcga ctccattagg aacattaaaa tctttaggta ctgtcaacat gattgaattg
     9301 ccttgttttc ttgtttttgt aatcatttaa atccctcatt tctaacagta tgtaattact
     9361 tagtaaatac attataccac gagataatgt atttacaagt aattactgtg actcgtaatg
     9421 cgtccttgct gaataaattt caaccacgtg ttcatcttca tctaccttat aaacaacccg
     9481 gtgttgtcta ttcaatcttc taaaatacct accttcatgt gtaggtttta acttctcaaa
     9541 tgattgggtt ctgttgcaaa gttttaaata aagaataaaa tcccttacgg tatctatgat
     9601 ttaagct
//
LOCUS       DACERL010000087         9564 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.092, whole genome shotgun sequence.
ACCESSION   DACERL010000087 DACERL010000000
VERSION     DACERL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.092"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(278..1588)
                     /locus_tag="IXF54_002419"
     CDS             complement(278..1588)
                     /locus_tag="IXF54_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1405643.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIRD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1628..2896)
                     /locus_tag="IXF54_002420"
     CDS             complement(1628..2896)
                     /locus_tag="IXF54_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1405644.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     LAKNITGPYELDPGFPMLTASDKPASTLQCTGYGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNAGRILPNADWCDTKCRPSMCRSGRRIYAGTHWLPIVYNRWSHPISFSTRKDLYDG
                     KLVTSILLICIRNHFASRLPTESFKNCIYW"
     gene            complement(2900..3310)
                     /locus_tag="IXF54_002421"
     CDS             complement(2900..3310)
                     /locus_tag="IXF54_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1405645.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHPTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(3307..4230)
                     /locus_tag="IXF54_002422"
     CDS             complement(3307..4230)
                     /locus_tag="IXF54_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1405646.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKLLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTIKFSEHNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(4227..5105)
                     /locus_tag="IXF54_002423"
     CDS             complement(4227..5105)
                     /locus_tag="IXF54_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1405647.1"
                     /translation="MQLNDFLGAPSSRRTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVAALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(5092..6429)
                     /locus_tag="IXF54_002424"
     CDS             complement(5092..6429)
                     /locus_tag="IXF54_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1405648.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETGRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKTAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSKQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(6432..7535)
                     /locus_tag="IXF54_002425"
     CDS             complement(6432..7535)
                     /locus_tag="IXF54_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1405649.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(7609..8862)
                     /locus_tag="IXF54_002426"
     CDS             complement(7609..8862)
                     /locus_tag="IXF54_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1405650.1"
                     /translation="MASDELGDIVTLQIAMLKNSSVRNALKSGVFWDVSEYLKDYKNL
                     SQISEDRIEAASIDGALYGVPIQKDYARAGLVIRKDWLDNLGLDVPTTMDELYEVAKQ
                     FTENDPDGNGVDDTVGFGDRAPSDIRYTSFKLFTSYFGAPNGWAVDDNGKFTPEFDTE
                     EYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGRTGIYTGMVDIKNLKTMGQGI
                     QDDMELVPVNKISDGEGGGYHVWSEGNGIGGLLAFPKSQVKDEAQLKKLLQFVDDLLE
                     EDVYKLMTGGIEGTHYELNDDGSIEFIDMDLWQQEVQPLSSSRPSEVTFSFKDTDPEK
                     EFSNQLIKENEEFAVMDPSVPLDSPTNNEVGSEIEKIVIDATVQYIMGQLDEDGFKKA
                     VEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(8881..9117)
                     /locus_tag="IXF54_002427"
     CDS             complement(8881..9117)
                     /locus_tag="IXF54_002427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405651.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASK"
     gene            complement(9083..>9564)
                     /locus_tag="IXF54_002428"
     CDS             complement(9083..>9564)
                     /locus_tag="IXF54_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405652.1"
                     /translation="NYRETLLMSWIYGAIGIVLLVDLSFIHNWYLRIALYIAAFIYVL
                     SALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGIYLMIIQTMPGVLT
                     FIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEKIYEKSNARKIRS"
BASE COUNT         2724 a         2093 c         1602 g         3145 t
ORIGIN      
        1 gatttcgttt ttcgaaatct ttcagcaact catttatcat aacttatcgt ttgttatttg
       61 tcaagaactt ttttggagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttaa
      121 cgacttagtt atcttatcac gtctaaaaac atgtgtcaac tcttttttgg aattttcata
      181 aaatgtttca atgaagcgcg acaacgttgt ttaatatatc attaaaaaaa ataaaggtca
      241 agctaaaata ttatttaagc ttgaccttat tataatttca acgattgtca aaagtattgt
      301 ttgcagctgt attgccagtt tctatagata aatcacagct tttgaaacat aagttttgta
      361 ctgcttcgat tcgcccgaat tcttttgcag ttatttggca atgagaaaag cagacatctt
      421 ctataggctt ttcaggaaat gctactaaat caaaagctcg ggaaggaagt gagtaacctg
      481 gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt gactttcgtc atttgctttg
      541 attcaggtat ttcttcagcc actgcttgcc aactttctgg catttgagca aggtcattgc
      601 tattttttct attgtattca ttatgccagt ccattatcca agaaaaagga aattgtacat
      661 caatcatatt cagattttct accctgatat tttcgatcac tccccctctt tcttttgaag
      721 atttcattcg aataccacag tctgactgaa aaaaatcgat atcgcgaata tatacgtcac
      781 taactccagc actaacttcg ctaccaatag ttacaccgta tcctgaacgg attttacatc
      841 tagcaacctc gattcttgat gattttcgat tgactcttgc accattgcca tccctgccag
      901 acttaatagc aatacaatca tcaccacagg ccaaatcaca ttcatagaca cgcacatttg
      961 ttgaagaatc aatatcaatc ccatctgtac ttggtccatc attgttgcgg atgattactt
     1021 tttctactaa tacatcgtta gaataagtga tttgcaagtt ccaaaaacct gatttctgaa
     1081 aaatcaaatc acgaacaaca acatgttctg catgatacag taaacaagcg cgtggacgtt
     1141 taatagcata atccgcaatc caacgaagtc ccttttgatc gtattcagct cttgtcccgc
     1201 ttttttgatc ttttccccaa tacagctccc accaatggtc tccttgaccg tcgattattc
     1261 cttttccttc tataagaata tctttcgccg agagaacatt caaaatcgct gctggccatt
     1321 tcatttctac gccggctact cgtgtatcga tttctggaaa tgcttcgata tctgttctcc
     1381 ccttgaggac tgcaccttct tcaaagataa gatgactctt atcttttaaa aaaagggctc
     1441 cgactaaata aatgcctttt ggaataaata caggtaagtg ttctttcgca ccttgatcga
     1501 ttgctttttg cagtattatt gtatcatctt gttgagtgtc gtttgtttta aagttcaaag
     1561 cattgataat cccttttttt gtccgcaata cgtttctccc tatctttcat tgattttttt
     1621 caattcttca ccaataaata cagtttttga acgattcagt gggtaaccta ctagcaaaat
     1681 gattgcggat acaaataagc agaatgctgg taacaagttt gccatcgtat aaatcctttc
     1741 ttgtactaaa actgattgga tggctccacc tgttgtagac gattggtaac caatgagtgc
     1801 cagcataaat ccgcctaccg ctcctgcaca tgcttggtct acattttgta tcacaccaat
     1861 cagcatttgg caaaatgcgt ccggcattcc agttcttctt tagctctcct tcaaatgtat
     1921 ccataaactg atgatctttt tcttgttgta cctcttcatt tgtctcaatc attatttctt
     1981 ccatcggagt tgtctggcta tctttaagtc ttaaccaatc gtcggacgtc caatacactt
     2041 cttgaatagc ggtttctctt cccaaaatag ccgcaccgtt taatggtctg gtacaaagat
     2101 atgtcatgta ccatcttcct gacggggttt gtactaagct cccatatcct gtacattgca
     2161 atgtagaagc aggtttgtca ctagctgtca gcataggaaa accaggatca agctcgtaag
     2221 gaccagtaat attctttgct aggcaaactg taactgaatg tcctcttcct gtaccgcctt
     2281 ctgccgtcat caaataataa tacccgttac gaaaatacaa atgtggtgcc tccgttttcg
     2341 ctaattctgt acctgcaaat attttataga tgggaccatc aagcttcttc ttctctgcat
     2401 catattcttg taatacgata ccgctggatt tattcggtgt gtccagacga taatcccata
     2461 gttcattgac aagccatttt tttcccgttt caagatcatg gaacaaagaa ggatcaaagc
     2521 cgttactatt taaataaatt ggttcggacc aaggacctgt gatctcagat gcagtgatca
     2581 aataattgtg gacatcttta aacggacggc ctgcactttt gacatctgtg taaacaagat
     2641 aaaacaatcc atcagcataa ctgatctgtg gcgcccatat acttccattt gtaggatttc
     2701 cttgcaaaga tacttgagtg gtcaaaatat ccgttgtatg tgaccagttg actaaatttt
     2761 tcgactcata tactcggatt cctggaagcc attcaaaaga ggagacaata atgtaatacc
     2821 gctctttcac tcttaatata ttaggatctg gattgaatcc tcttaaaata ggatttttca
     2881 ctttgatagt ctgcatgatt caggctcctt ttctcattct tactcgatat aaactgaatg
     2941 ccccagctgc gaaataaaaa aatgcaaaag gaaacgcaat aaactgagtg ccaatcaaca
     3001 atatgaatcc tgttataaaa tacaaaccag tggcagttgt taagtatttt cctttagcag
     3061 tcaaaaacaa tgctactgct aaaaaagcaa gtgaaataaa caacacaacg gcaaacagac
     3121 cagtcatgtc ctgtaataat ccaaatgtat tttctaaaga caaagttgga tgatctacag
     3181 caagcgactg ataacttttt ttaaactctg acgcagacat attacttaaa gtaactgcta
     3241 acccgccaaa gaaaatcaaa gatagactgc caccgaccat tcctagcaat agttcccatt
     3301 ttcttttcat ttctgaaccc cctgcttttc taaatgtatc cattcaggtc ttttgatcga
     3361 tgtttcataa ttgttattat gttcagagaa tttgatagtc tgttcatttt tcttgtcatc
     3421 ccaattatcc caaccagttg ggtgaacatg cccacctatc ttacacgctt caaataagac
     3481 atttgcatac ggacgccacg gtcttcctaa atagacgttt ctagtatcag gttctgcagt
     3541 caaggtacag ctttcaaaac gataccctgt tttttgattt ttcggcgtag atgctgctgt
     3601 gatgtatcca ggatttttac tggctaagct atggatatga cactgatgaa aaacggcatc
     3661 tgccccacca aatatgaaat ccaccgttcc ttcaatccag caatattcat aggtctgacg
     3721 acaatactca ggattggcag aagccggtgt acaaaaagga gtaccatctt tttgcaaatc
     3781 aggcaacgga cccgtgcata aggtatcctg ataaccagat agtcgacagt tttttaaaga
     3841 tacgcgatct cctgaattga ataaagcaac agcctgtcct accttttctc ctggacctgc
     3901 tgtattttcg atgtgaagat tttctaatac gacattcgat ccttctaaga aaaaagtagc
     3961 acttcggaaa gtccctaact ggctaccgtc aagatactcc tgttttgcaa aacgattacc
     4021 ggagataact actgttcctg ctcccacaag tttaaaattg gataatctgc tttctatata
     4081 ttcatcatat cttccttctt ttactatcaa gagctttgga tggctatccg gcaggcctgc
     4141 caaataatcg attccttctt ggatagtttc aaaatccgtc ggggtatcta tagcattctt
     4201 tccaattatc agtttgtctt tcatcctcat aaatgctccc ttatgaattg tttccaaatg
     4261 tttctcattt cttgtgtttc catatgaccg cctgttaaag aaaaagaaga aaagttttct
     4321 ggactaccca ttgattgata ctttttgtcc agcccttttc tcagcttcag tacgccttcg
     4381 tccaagcaca tcgtatcgtt ttttccaact aagctaagac gagggcgcgg agcgattttt
     4441 tcttggattg ctaacgttgt ataagctgtc aatagatttg gcacatagta ataaaaccca
     4501 tgatgatcta ggcggcgatg tagcaaaagt gtttcgatgt ccacctgtgc cgctagatca
     4561 atacaaactt tgacacgaga atccaatgct gccacccacc agcttaataa gcctcccata
     4621 gaaagaccaa tcgttgcgag ccgttgagta tctgtgtctt cccttgtctc caaataatcg
     4681 atcaagctga tcacatcata aatacgcatt ccccataacg tttttcctgt taataaaaat
     4741 tgtttgaaca actcgctttc agaaatacct ccgcgttctt caaatcccca agcatcaatc
     4801 gaccaaacag catatcccat tcctgtcagt acttctgcaa aagatggaga agctaagtaa
     4861 ggagcaccat ggatcaattc acttttcccg actgaaaaat ctccgccgtg agaatgcagg
     4921 taaaggaccg tcggaaacgg tccttctttt tctggataag catagagggc tggtactgtt
     4981 tccaattcat ttaagtacaa ttgatagtat tcaagcataa aaccgttttg tttttctcgg
     5041 tataatcgtt ttccactagg ggtacgtctg ctagatggag cacctaaaaa atcattcaac
     5101 tgcataagca aagtttttac gaacaaccag ttcatccata ttttctgatt gatttgattg
     5161 gaatttctca atactgattt ctttcgtatt ttctaataga aatgctggtc cctcgtgatt
     5221 ttcaatcgac atctggttaa aggaaacctt acctgcaaag ccaatgtaaa atccttgctt
     5281 gctcatttcc tctatccctg tcatcatagc agggacaccg ggaactgcat cttgggccat
     5341 tgaaacatca atattgtcaa atgtgatttc ttgtataaac tgttctgcta atccataaat
     5401 aaatcctgca gaagcatgga cattccttgc cgtgatattc gaaaaatgga tccttctgaa
     5461 tgctggtgtc cgctcatcta tcggataagc cgttttttcc catacgtacg gttctttccc
     5521 tcgcggacca cagaaataat aaagattcag tataaatgga cacatcacat tatccatgac
     5581 tatattggat actcgaatat cttcaacgat cccgcctcga cctcgtctag atttcaatcg
     5641 tattcctcga ccagtttctt ggaagataca atttgaaatg gtaatattac gaatactacc
     5701 actcatttca cttcctaata cgacaccgcc atgtccatgg accatcgtgc aatttgtgat
     5761 cgtgatattt tcgcaagcga tcctttcgta cgtatcttca gtccctgcct tgattgcaat
     5821 acagtcatct cccacgtcaa tatggcaatt actaatacgt acatttttgc atgattctgg
     5881 atcaattccg tctgtatttg gtgaatccgc tgggttcaag atcgtcaagt tatcaaacgt
     5941 tgcattactg caaagaatcg ggttgatcgt ccaacttggt gactggatca atttaatatc
     6001 tttcacagta attcgatgac agttatggaa actcatcaat ttaggacgag gataagcgag
     6061 attatctggt tcatttctaa aagtatgcca ccattttttt ccatttccat ctaatgtacc
     6121 gaaaccagtg acagagatgt tctctacatt ttgagcatag atacaggaag cataaacttt
     6181 tcgatggact ccttcccaac gtgaatgaac aacaggataa tcttttggat cgtcagagaa
     6241 tttcagaacc gctcctgcgc ttaaatgaag ttcgacattg ctttttaaaa ataaagctcc
     6301 tgttagaaat tcacctgctg gaacaactac ggtcccgcct ccgtcacttg ctgcagcatc
     6361 aatggcttgt tgtattgctt cagtattcaa ttcatcaatt gatgcgccaa atgtcaaaat
     6421 atcatacata tttataatgc ctccacttca atcaatgctt gcaagaatgc accaacgcct
     6481 ttttgatcat tacaaataat tggttcactg atgtagtaag catatgaacc atctcgctga
     6541 tctgctccgc ctaaccctgc tacttgacag tttttattca agttcagcca gccttctttt
     6601 gtttccagaa caaattcatc aagtaatcct tgatgtgatt tcttcacttg tggaagccat
     6661 tcttctttgt tcagcaggcc caaacgtatt cctttagcca ttgcgtaaac aaacatacta
     6721 ctgccggaag cttccagata atttcctttc cgttttgttt ctgttgttac ttgataccat
     6781 gtcccgctgt tttcttcttg atatggaagt aatgcagcca gtgtcttttc tagaatttct
     6841 ttcagcaaag aacaatctgt ttcttcatga agtaattcat aggtatctgc tgcagccatc
     6901 aaataccagc caatcgatcg gctccaaaaa tgatgggata gacctgtttt tggatcagcc
     6961 catggttgga ccttcttttc atcccatgca tgaatcagca aatttgtttt tggatcgaat
     7021 agatgtttat aactgatttc aaactgtctc aaaacatctt ggtaatcttt ttcttcacta
     7081 aaattctgga taaattcagc ataaaaagga gaacccatat ataagccatc caaccaaatt
     7141 tgataaggat aaatttcttt atgccaaaaa gctccttcgg atgtgcgtgg atggtttgcc
     7201 aactgctggg aaaggagttc tgctgctttt ttatattttt cttcgcctgt ttctttgtac
     7261 aataaaaaga aaagctttcc tgtatttaca tggtcgatat tgtattcttc aacgctgtag
     7321 ccacggatcg ttccatcttc ttgcacgaaa tagtcgatat tgtcttttat gaagtcaaaa
     7381 taaatttttt cttgggttcg ttcccaaagt aattggaacc ctttaagtac gacgccatag
     7441 tcataagacc attttccttt ataccacttt tcttcatata agcgaggtgt tctttccatg
     7501 atactttttg ctaatttctt tgaccagttt tccattattt gctctccttt aaaaattaaa
     7561 ataggatttg ggacaaatac caagaagtat ttgtcccaat cgtctatatt actgtgccgc
     7621 tgctttgtat gcttcctcgt actcttgtgt gattcgatcg cctccttgtg ctttccagtc
     7681 ttctacagct tttttgaatc catcttcgtc aagctgtccc atgatgtatt gcaccgtagc
     7741 atcaatgacg attttttcaa tttctgatcc tacttcattg ttcgtcggtg aatccaatgg
     7801 cacagaagga tccattacag caaattcttc attttctttg atcaattgat tagagaattc
     7861 tttttcaggg tctgtatctt tgaaagagaa cgtcacttca ctaggacgag agcttgataa
     7921 cggttgcacc tcttgttgcc aaagatccat atcgataaat tcgatagaac catcatcatt
     7981 caattcataa tgcgttcctt cgataccgcc agtcatcaat ttatacacat cttcttctaa
     8041 aagatcatca acaaattgaa gtaatttctt caattgtgct tcgtctttta cttgagattt
     8101 gggaaatgct agcaagccgc caataccatt tccttctgac caaacatgat aaccgccgcc
     8161 ttcaccatct gagatcttgt ttacaggcac taattccata tcatcttgaa taccttgacc
     8221 catcgtcttc agattcttga tatccaccat tccagtgtag attccagttc ttccttgtgc
     8281 aaattgttct tgctgatcag tcttttgggt aactgcaaag tcttgtgcca aatagccatt
     8341 ctcaaataga tctcttgaaa aattcaaagc tttcatatac tcttctgtat caaattctgg
     8401 agtgaatttg ccattatcgt ctacagccca gccatttggt gctccaaaat aagaagtgaa
     8461 cagtttaaag ctggtataac gaatatcact aggcgcacga tcgccaaaac caactgtgtc
     8521 gtccacacca tttccatcag gatcgttttc tgtaaattgt ttagctactt cataaagctc
     8581 atccatggta gttggaacat ctagtcctag attatccaac cagtcttttc gaatgactaa
     8641 gccagcacga gcgtagtctt tttgtatcgg tacgccgtac aaggcaccgt cgatactcgc
     8701 agcttcgatt ctatcttcag aaatttgact taaattttta tagtctttta aatattctga
     8761 tacatcccag aatacgcctg attttaacgc attgcggaca gaagagtttt tcaacatcgc
     8821 aatttgaagt gtaacgatat ctcctagttc atcagaagcc aatgcagttg ttaatctttc
     8881 ttattttgaa gcatcaggaa tccagttgaa tgtcagttcc gtatcggttt tttcttcgat
     8941 caagttcaca atcgtgtctg taggcggtga agcatgatgc aaaatattta accaagtgat
     9001 tttagctttt ccatcttctg tctctgaaga actgttactt gaactgctgc cgcatgcggc
     9061 aagtgctaga attcctgcaa aactaagacc taattttacg agcgttgctt ttttcataaa
     9121 ttttctcctt cactgtttta ataatatttt tgttttcttc tgttttttct gtatatgagt
     9181 tcttccattc caaatggcaa aatatagaat ttgctgtcca cgtgactgcg gaaaagaaga
     9241 gactaccacc gataaaggtt aaaacacctg gcattgtttg gatgatcatc agataaatcc
     9301 ctaccatact taatatcaac actaatgagt aatgaagatg tgagaatccg aataaaaatg
     9361 ccattttcat tttgaaaaaa tatcctttcc aatcataatg agccatgatt gggaaaatat
     9421 agagtgctga gagcacatat atgaacgcag caatatacaa agcgatccta agataccaat
     9481 tgtgtatgaa agaaagatcc acaagtaaaa caatcccgat ggcgccatat atccagctca
     9541 tcagcaaggt ttcacgatag tttt
//
LOCUS       DACERL010000088         9475 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.093, whole genome shotgun sequence.
ACCESSION   DACERL010000088 DACERL010000000
VERSION     DACERL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.093"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..848)
                     /locus_tag="IXF54_002429"
     CDS             complement(<1..848)
                     /locus_tag="IXF54_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1405653.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYAD"
     gene            complement(935..1639)
                     /locus_tag="IXF54_002430"
     CDS             complement(935..1639)
                     /locus_tag="IXF54_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1405654.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1839..2105
                     /locus_tag="IXF54_002431"
     CDS             1839..2105
                     /locus_tag="IXF54_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405655.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(2273..2503)
                     /locus_tag="IXF54_002432"
     CDS             complement(2273..2503)
                     /locus_tag="IXF54_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1405656.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2575..2730
                     /locus_tag="IXF54_002433"
     CDS             2575..2730
                     /locus_tag="IXF54_002433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405657.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(3039..3263)
                     /locus_tag="IXF54_002434"
     CDS             complement(3039..3263)
                     /locus_tag="IXF54_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1405658.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3530..3865
                     /locus_tag="IXF54_002435"
     CDS             3530..3865
                     /locus_tag="IXF54_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405659.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            4098..5039
                     /locus_tag="IXF54_002436"
     CDS             4098..5039
                     /locus_tag="IXF54_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1405660.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            5041..5931
                     /locus_tag="IXF54_002437"
     CDS             5041..5931
                     /locus_tag="IXF54_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1405661.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5974..6774
                     /locus_tag="IXF54_002438"
     CDS             5974..6774
                     /locus_tag="IXF54_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1405662.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6789..7640
                     /locus_tag="IXF54_002439"
     CDS             6789..7640
                     /locus_tag="IXF54_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1405663.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7637..7954
                     /locus_tag="IXF54_002440"
     CDS             7637..7954
                     /locus_tag="IXF54_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405664.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7947..8129
                     /locus_tag="IXF54_002441"
     CDS             7947..8129
                     /locus_tag="IXF54_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405665.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            8131..8601
                     /locus_tag="IXF54_002442"
     CDS             8131..8601
                     /locus_tag="IXF54_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405666.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            8598..9140
                     /locus_tag="IXF54_002443"
     CDS             8598..9140
                     /locus_tag="IXF54_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1405667.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            9137..>9475
                     /locus_tag="IXF54_002444"
     CDS             9137..>9475
                     /locus_tag="IXF54_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405668.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVL"
BASE COUNT         3311 a         1526 c         1920 g         2718 t
ORIGIN      
        1 ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta tgccattaac
       61 ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt ttcacgttct
      121 atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt ttgctgttct
      181 ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg aagttttaat
      241 tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt tattttatca
      301 aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag tttattgaga
      361 agtccagtta tttctgattc taatggtata agcattttct ttttaatatt attagcttct
      421 ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc cttttcttta
      481 atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc tatattcgga
      541 ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt aatttcatcg
      601 tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt tgacaagttt
      661 ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct agcatttaat
      721 tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt taatttttta
      781 gcttcagata atttgctact acccataatt attaaaacta ttccaactaa aaacaatagt
      841 actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca tataaaccct
      901 taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt ccgataattc
      961 taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa cgtaaaatga
     1021 ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca ccacctaatt
     1081 ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat acgaattgtc
     1141 cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag tcagcagcac
     1201 ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta agttctgttg
     1261 gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta ggaaaatcta
     1321 ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag gtagcaactt
     1381 tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga ttagatatag
     1441 ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct atctcattta
     1501 aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag gatgaacttt
     1561 ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct ctaagaaact
     1621 ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc gtttcggaaa
     1681 aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt tacgcaaaat
     1741 agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc gaagtatata
     1801 atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg aagaaagaaa
     1861 aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa ctgtcaagga
     1921 aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat aaaatgagga
     1981 acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga gtttccgata
     2041 tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca aaacaagtca
     2101 gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt gaagaatata
     2161 caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt attacggcga
     2221 atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat gattaatgtt
     2281 ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt atttgtccat
     2341 tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta ccatactcaa
     2401 cagcatctga tttattatca aatagttttg tatcttcgga attaccttca ccacgtacag
     2461 cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt cacctcgctt
     2521 tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga gattgtgagt
     2581 aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc agatgaaatg
     2641 gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat tgaacaaaaa
     2701 gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa tattatatca
     2761 cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg ccaaagtcca
     2821 cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag acgaaggagg
     2881 gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta cacaagatta
     2941 attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga tgcaactcct
     3001 gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg tgggtgtgga
     3061 tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat aaatagctca
     3121 gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt tgtattagta
     3181 tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt ggaattaggt
     3241 acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat attttatcaa
     3301 atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac aagagcatca
     3361 acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta gattgctagg
     3421 ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac tcagccgtcc
     3481 aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca tgaataagaa
     3541 aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg tttctattat
     3601 ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga caacagaaat
     3661 tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat tgtcagcaca
     3721 tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag aagaaaatca
     3781 accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg tgatgactcg
     3841 tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact cattatttta
     3901 tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt
     3961 accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga acgagttagc
     4021 aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg aagcgccaat
     4081 tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa gaatggctcg
     4141 aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga ttaaacaaat
     4201 acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg aaagatttag
     4261 aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg
     4321 aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca ttacatccat
     4381 ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg gaatgtaaaa
     4441 cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt agctatctct
     4501 gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc gctgttttga
     4561 tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg atcgatacaa
     4621 tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc gagcctgtaa
     4681 ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat gtagaagaaa
     4741 atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga gaactcaagc
     4801 ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc atcagtgagc
     4861 taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc tggaaacttg
     4921 ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat gtcgcaaatg
     4981 atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag atcaaataat
     5041 atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa acaagttgca
     5101 ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa gaaatttgaa
     5161 gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct tgtatctaat
     5221 gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt aactgctgca
     5281 acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc attcaatagt
     5341 aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg ctataaagga
     5401 tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat cgaagtttac
     5461 gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga tccaaatggt
     5521 agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa tggattcaaa
     5581 aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa caataaagat
     5641 cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa
     5701 actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc
     5761 accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat tgctgaaaca
     5821 gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa
     5881 gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata atgagggagc
     5941 tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt caaattacat
     6001 cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt atggagcttg
     6061 tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa agagatacaa
     6121 gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat gaacgaaggc
     6181 gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa acaatttgaa
     6241 aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc aataaaaaac
     6301 tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac aagtgaacaa
     6361 caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa taaaacatca
     6421 tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta cattctcgcg
     6481 gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt
     6541 tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg
     6601 ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt atctgctaaa
     6661 aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta taaaacaaaa
     6721 caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat ttgagaggag
     6781 gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc
     6841 aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg aagaaccctg
     6901 atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc
     6961 aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga gcaatcaatt
     7021 ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt
     7081 atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc tttgttaatt
     7141 cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga acaggtaaaa
     7201 gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac gataaaaaaa
     7261 tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac aacaatcctg
     7321 aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg gatttagtca
     7381 tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga gcgtttggca
     7441 taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt atcacaacga
     7501 acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca agaatgtttg
     7561 tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc ataaaaccag
     7621 tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc gtcagataat
     7681 gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga gtcgaatggt
     7741 tcggaagata agtaagctaa tcggtgcaga tacgatggat cgatccaagc cattacccaa
     7801 actgaatttt gaatcattta cgtttgagaa atatagccat ctgagagagg taggctatag
     7861 tgtaaatgct attcgaaaag cacttggggt aggaagtagt gtatgggcaa aatggcggaa
     7921 agaaaatgta aatgaggtaa atcaaaatga ttaagctaat aggcaaagag acgctcaagg
     7981 tttactatgt agcggaaaca aaggcagaag tcagccgttg gctattgaaa atgtttaata
     8041 agactagagc caatgcttca gtaagtcaga aagcaagtgt atcagaaaca ggcatgcctg
     8101 aagcaatgtg gatttcaagg aggaactgag atggcaatga taccgaagtt tagagcgtgg
     8161 gatagagaaa aagacagaat gatttatcct agcacgacag gagtttgttt tgaaatgaat
     8221 gatgatggta taagtgtgct tgatattgat gtagattatc ccaatgacta tggttttccc
     8281 aaaatagctt caatcctcat gcaatctacc actctgaagg ataagaatgg tgtggagata
     8341 tttgaggggg atatagcgct tatcactcat aaatcaacga actacgccga tacttattgg
     8401 cacagttacg tagaagtttt tagagcaaaa aacggagcct acagaattcg cggtgaacac
     8461 atctacgaaa cagaattata tagtaataga aaacaattga cggtagcggg gaatgtttat
     8521 aattcaccag aacttttgaa gagagatcga tttatgacta ctaccgaaaa tccagagtta
     8581 ttgaaggagg aacagcgatg aataaacagg aattgattga taaatatact gcagaaatat
     8641 ctagattaag accttattgc cctaatagac atttaattag cgatcaactt aaatatggtc
     8701 tttataaaga aatcctagaa gatttaaaac aactagacga accgcagaaa ccagcggtgc
     8761 ctaagtttgt ggcggaatgg atagaatacg ctaaaaagaa aggcgatagt ctagctattt
     8821 cattcaagcc gtggaacctc tacggtgttg agtatagcaa gactgataga tggattgaag
     8881 ataatcaagg aacgtttgct cgtgcttggc taattggcta cgaggtcgag aaagagccgt
     8941 tgtattatgt gaagttgcca gttgtgtatt ttaatcattg ggatttagag acgtatctaa
     9001 tgaaagatga tagaggaaat ataacaattg cagacaacaa cgattttgat gatatgaaat
     9061 ttacggaatc agaaataaaa gcaattgatg aaagatactg gccatttgct gtgccagtgg
     9121 aagaggtggc ggaaggatga gcaatcttaa agttggagat attgtttttt acaaaggtga
     9181 cgaaggcatc atccgagaag ttatgacgga tggaaaagaa aatttttata gggtagatgt
     9241 cagcagaaaa tctatactct acctgtatga agacgaatta gatttaggta ctcaaaaact
     9301 caacgacaac cagaaaatgg tgtttgatta tcttaaatca aacgttgaac aaggtggcaa
     9361 atcagttatg tattcgatat tccttctagg tgattgggac tctcgaattg gagcagcaag
     9421 aaacgttgat atagcttact gtggattaaa tggtaagcag gaagcagaag tccta
//
LOCUS       DACERL010000089         9378 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.094, whole genome shotgun sequence.
ACCESSION   DACERL010000089 DACERL010000000
VERSION     DACERL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.094"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..699
                     /locus_tag="IXF54_002445"
     CDS             <1..699
                     /locus_tag="IXF54_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405669.1"
                     /translation="DLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNKR
                     INLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTNS
                     GGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGPK
                     VHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKK
                     CSYAYVKAIKAVVR"
     gene            696..1007
                     /locus_tag="IXF54_002446"
     CDS             696..1007
                     /locus_tag="IXF54_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405670.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            997..1350
                     /locus_tag="IXF54_002447"
     CDS             997..1350
                     /locus_tag="IXF54_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1405671.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            1340..1741
                     /locus_tag="IXF54_002448"
     CDS             1340..1741
                     /locus_tag="IXF54_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405672.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1734..2138
                     /locus_tag="IXF54_002449"
     CDS             1734..2138
                     /locus_tag="IXF54_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405673.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            2150..2758
                     /locus_tag="IXF54_002450"
     CDS             2150..2758
                     /locus_tag="IXF54_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1405674.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            2778..3140
                     /locus_tag="IXF54_002451"
     CDS             2778..3140
                     /locus_tag="IXF54_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405675.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            3143..3325
                     /locus_tag="IXF54_002452"
     CDS             3143..3325
                     /locus_tag="IXF54_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405676.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            3342..6773
                     /locus_tag="IXF54_002453"
     CDS             3342..6773
                     /locus_tag="IXF54_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1405677.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            6824..7561
                     /locus_tag="IXF54_002454"
     CDS             6824..7561
                     /locus_tag="IXF54_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1405678.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            7571..>9378
                     /locus_tag="IXF54_002455"
     CDS             7571..>9378
                     /locus_tag="IXF54_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405679.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFT"
BASE COUNT         3295 a         1395 c         1980 g         2708 t
ORIGIN      
        1 gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg gtggaaatat
       61 ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc ggatgtcatt
      121 ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg gtattgtagc
      181 gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg agaaaatatt
      241 ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac tataaacact
      301 aattcaggag gcgcacagtg ggttattaac cgtcctaaag cgaattattg gaaaaaggtt
      361 tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa tatacaaatc
      421 tggacagccg agatgtgggc acagctttgg aacatgatgt atttcaatat tggtcctaaa
      481 gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt taaagaagta
      541 aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt caaagggaga
      601 tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa aaaatgctct
      661 tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa gtgactgaag
      721 aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa gatgagtcaa
      781 ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt acaacacttt
      841 ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga tacgactgga
      901 atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt ttcattcaac
      961 attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg tagactcgaa
     1021 gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta
     1081 ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag aaatctttcc
     1141 atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac taagaaagta
     1201 aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt tatcgtaatc
     1261 gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat ctatttttat
     1321 cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac gtttaaataa
     1381 gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac tctttcaaga
     1441 ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg aaacaggaga
     1501 aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc taactttcta
     1561 ttcagaaaat agagaagatt taacaggaga ctcactagat attatttcat tgattcaaaa
     1621 tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga accaagatcg
     1681 ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt gtcatggcta
     1741 aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg atggaacgct
     1801 attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt tcggctattg
     1861 cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga agagggcacc
     1921 aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt ttcatcatta
     1981 gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt acttctaaaa
     2041 gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat ccaattacag
     2101 aacttctgat tcgtcaattc gataaattaa ataaataggg ggaacaacaa tggctaaaac
     2161 aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt ttaattttaa
     2221 gaacgcagga aatgcaatcg caacgaattg taatggacaa ttagatggcg aaacagaaat
     2281 gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa tcaaaatcta aaccaatcaa
     2341 tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgtt tcaatgggtt
     2401 gaaacaagat ggacgtatta aaccaggcat ttactcttac ggtcctgatt ccgtaggcga
     2461 agatttctca cttgctgcag agatcgtgga tgacttcgaa gaaaatagaa agttagttgg
     2521 tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga
     2581 agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca
     2641 tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc
     2701 tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa
     2761 aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa
     2821 ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta
     2881 acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc
     2941 aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat
     3001 tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat
     3061 tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg
     3121 aaaaaaagct tcaaaagtaa aaattccaaa gctcttagtt gaaacgtggg tcgatgtcta
     3181 ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt
     3241 agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc
     3301 aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag
     3361 aagcagaagt aactttcata gctaacgatg acggattgaa atctacgtta aaagaaatca
     3421 gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga
     3481 ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat
     3541 tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgcgaaa aaatattatg
     3601 gagaaaattc caccgaagtt cagaaacttg aaagagaact gattaatcaa caaacagcgc
     3661 aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg
     3721 aaatacagac gtacgagtct acaatgcaac agttggatag cgaacaaaaa aatgttcaag
     3781 ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag
     3841 cttctgaagc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg
     3901 cggagaaaac gatagatatc ctaagacgac agttagaagc tacacagtct gaatttggcg
     3961 ctacatccac agaagcaatg cagatggaag cgaagcttaa tgatgctgaa cgtgaatttg
     4021 aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca
     4081 aaatagatat gaataattta atggaagctt ctgacgtttt aagcgacatt ggcgataagc
     4141 ttacagaatt agggaaacaa gcagtggact ctgctaacag tgtaggtagt tcccagagta
     4201 agatacaagc taattttggt ttgtctaaac aagaggctga agaattaacg aatgtagcca
     4261 gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt
     4321 tggtaaagcg taatttagga gatttaaata atcaagattt acaaaatatc acggaacaag
     4381 ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg
     4441 gcttaatggt caatttcggc ttgagtgctc aagatgcaat ggatttaatg gtttcgggta
     4501 ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat
     4561 tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag
     4621 atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt
     4681 taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt
     4741 ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag
     4801 attttagcaa tatggatggt caatacgacc aattaaataa agcttcgaca atttggtctg
     4861 cgcttggcga agataatgcg atgaaagttg tccaatcttt aactgatgtt aaccatacat
     4921 ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttgcaag
     4981 agttgaacgg gaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca
     5041 tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc
     5101 ttccacaacc agtacaggat ttcattgttg ctataggtgg tcttacagca gcatttggca
     5161 tactgttacc ggcaatacta gccgtgtcat atttatttgg tccgatgatg cttataattg
     5221 gaggaattat agctgttata gcaggcgtta ttgtggcaat taagaactgg ggtgcaatta
     5281 ctgactggtt tagtggttta tggaaaaaat ttactgattg gttgggtgat acttgggaaa
     5341 gtataaaaga cggagcctca tcagtttggg atggggttaa agaaacctgg tctggatttg
     5401 tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg
     5461 gattagttga aggagcttcc aacatctggc aaggagtcca agaaacttgg caaacattcg
     5521 ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag
     5581 acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt caagctggta
     5641 ttaatattat cgtgggtatt tttgatgtag ctggacagtt attgggcgca gcttggcaag
     5701 ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg acacaatgga
     5761 taagtatcgc ttggaatggg attgtaacta cattccatac tatatttgat ccagtagtgg
     5821 catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa
     5881 ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac
     5941 aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa
     6001 tttggaattc aatcagctct acagcatcaa atatctggaa tagtgtgact agtagtataa
     6061 gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt
     6121 ggattagcgg aatttggaac ggtatcaaaa atactgcttt gaatctttgg aatggaatta
     6181 caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca
     6241 atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacatat
     6301 gggaaggcat caaaaagact gttagcgctc caattgattg gattagagac aaaatcagtg
     6361 gcatttttga taatttgaat atttctatac cacatattcc gttaccggaa tttatcatgg
     6421 aaggcagctt taacccgcta aaaggtcaaa tcccccattt gcgtgtaaaa tggcatgcta
     6481 aaggaggtat ctttactaaa ccaactttac taggtggaat gaacggtgtc ggtgaagcag
     6541 gacctgaagc agttttacct ttgaaaagat ctgttttgca agaaattggt gatcgtatct
     6601 tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat
     6661 tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa
     6721 tcattgatag cattacaaaa attcaaaatg ataatgcttc ggcatggcgt taaacaggag
     6781 agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca
     6841 ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac
     6901 cataccttct ccaaaaagac agagagttga aacttctgtt ttaggtaggt taggtggctt
     6961 agtacaagat tactcgtttg aagatatgtc gtttacattg cactataact atttagagga
     7021 tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaacta
     7081 tgcaaagaaa ttagaattct ctgacgatcc caacgtctat tacgttatcc agactatcga
     7141 tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc
     7201 gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt
     7261 taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc
     7321 tacttcatgc cagttcatct taaatgatta tgtgtttagt ttcgaaggct tagtagcagg
     7381 aactgatgta gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga
     7441 tattttagat cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat
     7501 caatcatttt aattgcactg gtttgagcaa aatacaaatt tatcgtaacg ggttaaggta
     7561 ggtgaaatag atgatcgata atttaataac tatttacgat aaaaatgacg cgaataattt
     7621 agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac
     7681 tgttagcaat aaactaaacg gagccgagat atttcagggt acttattcaa taaacggaac
     7741 taatgcagat ttgattgtag acggacgtat tattcagtgt tatgtagatg aaaatcgagc
     7801 aaaacagcgt ctacggattt attatgcaaa aacttctgta ataggcaata cgatagaagt
     7861 aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat atgacagcgg
     7921 aacagaaaag atcactgcta ctcaggcatg gcaaaacgca aaagttttag cgaaaccagc
     7981 tatcccttcg cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga
     8041 aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca
     8101 tggtgagttt ctaaaagata ataacacatt acgtcatgaa aaaagtctag gtacggatca
     8161 taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca
     8221 aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat
     8281 cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt
     8341 ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa
     8401 agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt
     8461 agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat
     8521 gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt
     8581 ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa
     8641 aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga
     8701 tcaattgaaa aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac
     8761 tgatatgatt actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag
     8821 tcgtattttg attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa
     8881 caatgctggt attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac
     8941 gttagatggc ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat
     9001 tagaggtact acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa
     9061 aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta
     9121 tgtaagtgct gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat
     9181 tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt
     9241 tctatctttt ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga
     9301 cggtagttct ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg
     9361 taggactatg aaatttac
//
LOCUS       DACERL010000090         9180 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.095, whole genome shotgun sequence.
ACCESSION   DACERL010000090 DACERL010000000
VERSION     DACERL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.095"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            239..1024
                     /locus_tag="IXF54_002456"
     CDS             239..1024
                     /locus_tag="IXF54_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1405680.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1097..1744)
                     /locus_tag="IXF54_002457"
     CDS             complement(1097..1744)
                     /locus_tag="IXF54_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1405681.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1752..2438)
                     /locus_tag="IXF54_002458"
     CDS             complement(1752..2438)
                     /locus_tag="IXF54_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405682.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2444..3517)
                     /locus_tag="IXF54_002459"
     CDS             complement(2444..3517)
                     /locus_tag="IXF54_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1405683.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3739..4413
                     /locus_tag="IXF54_002460"
     CDS             3739..4413
                     /locus_tag="IXF54_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405684.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4418..5758
                     /locus_tag="IXF54_002461"
     CDS             4418..5758
                     /locus_tag="IXF54_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1405685.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5907..6893)
                     /locus_tag="IXF54_002462"
     CDS             complement(5907..6893)
                     /locus_tag="IXF54_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1405686.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            7617..8648
                     /locus_tag="IXF54_002463"
     CDS             7617..8648
                     /locus_tag="IXF54_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1405687.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            8757..>9180
                     /locus_tag="IXF54_002464"
     CDS             8757..>9180
                     /locus_tag="IXF54_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1405688.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         2932 a         1680 c         1566 g         3002 t
ORIGIN      
        1 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
       61 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
      121 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
      181 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
      241 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
      301 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
      361 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
      421 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
      481 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
      541 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
      601 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
      661 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
      721 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
      781 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
      841 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
      901 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
      961 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
     1021 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
     1081 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
     1141 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
     1201 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
     1261 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
     1321 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
     1381 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
     1441 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
     1501 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
     1561 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
     1621 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
     1681 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
     1741 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
     1801 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
     1861 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
     1921 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
     1981 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
     2041 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
     2101 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
     2161 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
     2221 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
     2281 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
     2341 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
     2401 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
     2461 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
     2521 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
     2581 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
     2641 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
     2701 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
     2761 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
     2821 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
     2881 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
     2941 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
     3001 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
     3061 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
     3121 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
     3181 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
     3241 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
     3301 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
     3361 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
     3421 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
     3481 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
     3541 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
     3601 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
     3661 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
     3721 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
     3781 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
     3841 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
     3901 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
     3961 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
     4021 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
     4081 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
     4141 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
     4201 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
     4261 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
     4321 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
     4381 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
     4441 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
     4501 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
     4561 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
     4621 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
     4681 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
     4741 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
     4801 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
     4861 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
     4921 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
     4981 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
     5041 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
     5101 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
     5161 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
     5221 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
     5281 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
     5341 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
     5401 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
     5461 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
     5521 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
     5581 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
     5641 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
     5701 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
     5761 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
     5821 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
     5881 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
     5941 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
     6001 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
     6061 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
     6121 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
     6181 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
     6241 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
     6301 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
     6361 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
     6421 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
     6481 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
     6541 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
     6601 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
     6661 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
     6721 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
     6781 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
     6841 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
     6901 cctcctagca aatagattta tgattggtat attgttactt ataaatttat tctataggag
     6961 acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta attgaaattt
     7021 gaaaacattt atgttaatga aagtcatatt ctaataaaat gtcatttgtt tctatatgaa
     7081 tttttatgaa aaatttcata taatttccac tcgttgtgat ggtttaattt atccaatcca
     7141 tcaataagca aacaacttat ataaaaaagg attaattctc aatataattg ctcctaccat
     7201 agaataaatc ttcatgtaaa gtaatgaagt accaactatt tctaatactt ctttaagaat
     7261 tatcccaaca atatcgttag acaaaccatt attgaataaa tggagagcca atcgattctg
     7321 aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc ctcctgccat tagaacgaac
     7381 caaatccatt tcattgctca tgaagtaacc acatagctaa aaagaggata aatcaattaa
     7441 aagatcaaaa gaatgactgc actaataaaa taactaaaaa tgtatcttct tacttcatat
     7501 cttttagcta gtacttagtt aaaaatttgc ttcctaagat caagacaaac tgtctatctt
     7561 catttgatga aatgcggtaa aatatagaaa atgaacacta ttggagggaa tttgtaatgg
     7621 acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca acgtacacac ttgctccctt
     7681 ccgtctttca acagttttta gaagaaaatc gttcttccct ttcattaggc agtcgctatg
     7741 aatacgcaaa agattttcat ctgtttactg attatttgag aaaagtattt tctgaagaag
     7801 tggatgatca gctgattatc catttagaaa aacaagatta tcagaatttc ttagctaaaa
     7861 tctatcgaca tactcgttcg ttccaaacta cagcgaacca agaaacggag caattgtttg
     7921 aaaatagcta ttacgggatt tcaaggaagc aatattcatt aaatcatttt ttacgatttt
     7981 tgtatcaaaa aggactgatt gaagaagaga tttctctttt gggtacaagt ttaccggaaa
     8041 caaccaaagc agctccgcga tatatcgatg cagaactttt caaagatttt gagcatttgt
     8101 ttatcccgat tgaaggcttt caaacaagca gagaagctag ctttgaagaa aagaatagac
     8161 cgaggaacct tgcaattact gccattcttc tgtattgcgg attaaagatc catgaagtag
     8221 tagaacttaa acgtaaagat gtatctttga ccaagcagat tcttaatctg aatagaaaag
     8281 aaaacagact gaataaagta ccaataccaa aagaagcgat gccatttctg gagaactatt
     8341 ttaagttaac agcacaaaat acggacgaaa attcttttgt ttttcgttcc tcacatgatc
     8401 agcaaatttc tcctcggact attcgccaaa ttttgagcaa aattacagta tataaaaacg
     8461 tatcgccaga actatgcaga aaaacatatc gttattatgc agaactatat ttagatgatg
     8521 ctgacgcggt aaattattta tgtggtaatc gatacctctc atcccattct tttcaagata
     8581 tctgggaaaa gatggttgat ttttcttatc acgaattagc ggcattcgtt cgggtttctt
     8641 ccatataaaa caaatgtatg atcttttttt cacaataaac tatgttatta tataaaggat
     8701 tatgttacga ttatcttgaa tttgtatatt acttaacaaa agataatgga gtgtgaatga
     8761 tggagaccat aacgtattcc cctttgaact tatttacgaa ttttcaagaa gctgcagcta
     8821 acttttccga gaccccgatt atctttgatg agccactttc agctttccca gaattaggac
     8881 ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat caattggctc
     8941 atcttggagt gaaatccgga gacaaagtta ttatttataa gagttccgct tttgacacat
     9001 accttctagc tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc
     9061 taccaacaac aacaatggaa gtatttatcg atcgtttaga agaccctttc attttatttg
     9121 atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca aaacagattc
//
LOCUS       DACERL010000091         8966 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.096, whole genome shotgun sequence.
ACCESSION   DACERL010000091 DACERL010000000
VERSION     DACERL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.096"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(57..350)
                     /locus_tag="IXF54_002465"
     CDS             complement(57..350)
                     /locus_tag="IXF54_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1405689.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IXF54_002466"
     CDS             complement(378..1043)
                     /locus_tag="IXF54_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1405690.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IXF54_002467"
     CDS             complement(1183..1815)
                     /locus_tag="IXF54_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405691.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IXF54_002468"
     CDS             complement(1822..2889)
                     /locus_tag="IXF54_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1405692.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IXF54_002469"
     CDS             complement(2886..3617)
                     /locus_tag="IXF54_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1405693.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4262)
                     /gene="greA"
                     /locus_tag="IXF54_002470"
     CDS             complement(3786..4262)
                     /gene="greA"
                     /locus_tag="IXF54_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1405694.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="IXF54_002471"
     CDS             complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="IXF54_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1405695.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5777..6946
                     /locus_tag="IXF54_002472"
     CDS             5777..6946
                     /locus_tag="IXF54_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1405696.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7213..8835)
                     /locus_tag="IXF54_002473"
     CDS             complement(7213..8835)
                     /locus_tag="IXF54_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405697.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1700 c         1333 g         3077 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc tgcatctaca
     4021 ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt gattcttcct
     4081 tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga aagatctccg
     4141 aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt ttttagttct
     4201 tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac tttctctacc
     4261 atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt gaggaatata
     4321 gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa aatgatcaat
     4381 gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc catatgttct
     4441 tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa ataataatag
     4501 tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt gtcaaaaggt
     4561 ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc tgtatcttca
     4621 tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc tgactgaagt
     4681 ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg atcattttct
     4741 tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac agtcaaattt
     4801 ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt caccatttca
     4861 atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg gaataagtaa
     4921 ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag ttctgggaat
     4981 ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa ttctttcttg
     5041 tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta cccttctgga
     5101 atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc ttgcaactgt
     5161 ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc ttgaaagcca
     5221 gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat aatattgtcc
     5281 ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc gacttggact
     5341 aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg atacacggta
     5401 aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat gatcttcctc
     5461 acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc tgctgtagct
     5521 gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga caaaacaact
     5581 cctccttact atcaagttcg taactttatt cattatacat gatatttgaa ttggcgtgaa
     5641 tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact ataagattaa
     5701 aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa aaagaaagga
     5761 gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat attcattttt
     5821 aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta ttcttggatg
     5881 gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag ctcaaaaaaa
     5941 gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac tattaagaaa
     6001 aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg
     6061 ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt attatcagat
     6121 tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg agcgtgggca
     6181 cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca gccagcaaat
     6241 tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt ctccaaccat
     6301 accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat ttacagatta
     6361 tcagacctct cattatgatt cattgaactt taccatattg actggaattt tgatcaaatt
     6421 aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt taggattaca
     6481 gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat cctatcaaat
     6541 gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga tccataacga
     6601 attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt ttacacaagt
     6661 tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg aaaatgcgaa
     6721 tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt cgcaaggaaa
     6781 tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg ctatcatcat
     6841 ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc taagaaacca
     6901 aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg atgaaaagat
     6961 agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact aaacgtagga
     7021 aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc caaataaaca
     7081 gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat ttctctttac
     7141 caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat tgctaaaata
     7201 tgtgctagca aatcattcat ggcttgttct tatttttaat tttatgcaat aaaaaagaag
     7261 aacaattagc gtgattatag aaataaataa tgagatttta gaaaaaaatg tgtatgaata
     7321 cctgattgtt actttattta ttccactttc actttttact gtaacaaaag gacctccttg
     7381 ttttagtact gtagcatttt ttgaattaac agtaaccgtt aatcctttat accggaatac
     7441 aggtaaaact attgttttgc cttttgaaga tttaagcgta aatgtaattt catctttatt
     7501 tggagaaaag ctactataaa taacttgacc atcttctgta acttgatggt ttttcaaaag
     7561 caattgatct tttttatagc ctataggtac ataatcaaaa ttctcaaatt cttcaagttt
     7621 attttccatt tgttcattag aataagttcc ttgaaaattc atttctttca ccatgttaac
     7681 tactgatgaa taatttaaaa ttgaaatagc taatattaat aatagtataa aatttcttct
     7741 ccttgtataa gaaaattttt ccaactgtgg agaaataaat agagatactg aaaaagatat
     7801 gaacaatgtt gaaaatatca taaaacgcca aggagcttga ataatattta aaggagtatt
     7861 ttgtaaggca aaccatggaa atatttttgt acaaagtaga atagatatta tacccattat
     7921 ataagtgatt ttagataaga ttcctaattt tttaaagtta aatgttagta taattaaaca
     7981 aataagcaaa gttaagccaa taccaaatga tagtaattct gaatctatac ttccaatcaa
     8041 aaactcttta cccagataaa ttgctctttt ttgtaatata tccacataag gtctattcag
     8101 atcttgatac aatgtctgct caatcatagg acctataaac gctagtgaaa gaagtaaagt
     8161 aactatagca gcttttatta atttaataat taagaagatt tctattttct ttttccgata
     8221 aaattgaata attaaagcaa ttataatcat taccatagtc atagctacag aaagaacatg
     8281 agtgtaaatt aataaagtca tgctaatcgc caacataacc caagattctt tttttgagtc
     8341 gtaaagcttt ataaacccga gtaaaataag aggaagtatt gacattgcga gtaattcccc
     8401 cattgcccct cgaagaaata tttctacact cctaaaagaa gatgttgtat atataatagc
     8461 gaaaaaggag ctagtaacat gttttttggt aatttcatac attacataat gacatataaa
     8521 tagagtaatg agcgtaacaa tataaagaaa aaggttatag cctattgcta aattagtaaa
     8581 cttgatcaat aaaaataaag gatatagagt taaccatggg taaaaattat ttacttgcat
     8641 gccatatgat ctaaacgttt caaaattaat aggggagcta aaaatagatt ctaaacttaa
     8701 cgcacgatta atgtgaaatg ccgtatcata aaagccaaat atttccatgt ttttaaatgc
     8761 tggaaataaa tataataaac taacaaatgc ataaactata aatattttta cagaaaaaat
     8821 ttttttcaaa ttcatatacc acctcatgtt tttttatatt acatatagta tcatttattt
     8881 ttttaaaaca ctagattagt cactatataa aatgtgatta attttattat taataaaata
     8941 ttttttatat tacaaaagta gtgttt
//
LOCUS       DACERL010000092         8850 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.097, whole genome shotgun sequence.
ACCESSION   DACERL010000092 DACERL010000000
VERSION     DACERL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.097"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(324..893)
                     /locus_tag="IXF54_002474"
     CDS             complement(324..893)
                     /locus_tag="IXF54_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1405698.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IXF54_002475"
     CDS             complement(967..2256)
                     /locus_tag="IXF54_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405699.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IXF54_002476"
     CDS             2610..3458
                     /locus_tag="IXF54_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1405700.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IXF54_002477"
     CDS             3472..4545
                     /locus_tag="IXF54_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1405701.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IXF54_002478"
     CDS             4535..6706
                     /locus_tag="IXF54_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1405702.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8839)
                     /locus_tag="IXF54_002479"
     CDS             complement(6818..8839)
                     /locus_tag="IXF54_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1405703.1"
                     /translation="MANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPII
                     LFSPMMGMEFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIA
                     MGITVAYIYSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQ
                     KLAELLPESVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESA
                     VTGESRGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLE
                     SLSDKVAKWLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVV
                     ARSTSIAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNE
                     ILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVN
                     EKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKE
                     RKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGI
                     NDAPSLARASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWW
                     GAGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         2603 a         1795 c         1595 g         2857 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt
//
LOCUS       DACERL010000093         8737 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.098, whole genome shotgun sequence.
ACCESSION   DACERL010000093 DACERL010000000
VERSION     DACERL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.098"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            139..1026
                     /locus_tag="IXF54_002480"
     CDS             139..1026
                     /locus_tag="IXF54_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1405704.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            1023..2192
                     /locus_tag="IXF54_002481"
     CDS             1023..2192
                     /locus_tag="IXF54_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405705.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2216..3571
                     /locus_tag="IXF54_002482"
     CDS             2216..3571
                     /locus_tag="IXF54_002482"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1405706.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3643..5448)
                     /gene="glmS"
                     /locus_tag="IXF54_002483"
     CDS             complement(3643..5448)
                     /gene="glmS"
                     /locus_tag="IXF54_002483"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1405707.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5857..6147
                     /locus_tag="IXF54_002484"
     CDS             5857..6147
                     /locus_tag="IXF54_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405708.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6160..7185
                     /gene="sppA"
                     /locus_tag="IXF54_002485"
     CDS             6160..7185
                     /gene="sppA"
                     /locus_tag="IXF54_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1405709.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7188..7847
                     /locus_tag="IXF54_002486"
     CDS             7188..7847
                     /locus_tag="IXF54_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1405710.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            8004..>8737
                     /locus_tag="IXF54_002487"
     CDS             8004..>8737
                     /locus_tag="IXF54_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405711.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSV"
BASE COUNT         2759 a         1596 c         1933 g         2449 t
ORIGIN      
        1 gagaaaaata aaacgcatac ttgcaatttt cttgaaaatt atgcgttttt ctcaagaaaa
       61 atacgagcat atcagcctct aaaattaaaa catatgatat actagagagg aaatctcaaa
      121 agagaagaga ggctttttat gtcatttcaa atcggggaac tttttaattt ggactattgg
      181 cagcaaatgt tttccggtga cttgttctct ctgaattttt tggtgaacat acttgatatt
      241 ctggttgtct ggtatcttgt atacaaactg atccaactgc tgcgaggcac aaaagcgatt
      301 cagcttctga aaggtgtagc tatttttatt gttatccgaa tcgtagcgga aatcattggt
      361 ctgcacacct tgtcttggct gatgaaccaa gtcattacgt acggggtgat agctgctatc
      421 gtgatttttc agccggaaat ccggagagga ctggaacatc ttggacgcag ttcgcttttc
      481 aaacaatcaa aaagcgaaca gcaggaagat gagaagatga tcttgtcttt tgacaaagcc
      541 atccaatata tgtccaagcg aaaaatcggt gcgttgatca ctatcgaacg tcatactgga
      601 ttagatgagt atattgagac agggattgct ttagatgcag atatcactgg ggagctgctg
      661 atcaatatct ttattcctaa tacaccgctg catgacggtg cagttattgt aaaagaagga
      721 aagatcgcag tagcaagcgc ctatcttcct ctatcagaaa gtatgttgat ccctaaagaa
      781 ttcgggactc gacaccgggc agctgtcggt ataagcgaag tcagtgacgc gattaccatc
      841 gttgtctctg aagaaaccgg ggacgtcagc atcacgttag ataatgaact gatggctggc
      901 ttatcccagc aagaatattt ggcaatcttg cgtagagaac taattgctga agagaaaaca
      961 aaaaacaaga aaaacgtgat tcaatctttc ctagacggta tgactaaggg aggggatcgc
     1021 aaatgatctc aaaagagaaa cggacaagca ttctttacag ttttattgca ttgctgttca
     1081 gtattgcgct gtacttcaat gcaaatggcc agtccgttcc aaatacgctt tctggaaatg
     1141 aagcgtataa tcaaacagtt acgggggtgc cgatcaagat ttcttatgat tcaaagcgct
     1201 actatatcca tggatatgaa aatgcagtga ctgtcaaact ttccagtgca aaccgagttc
     1261 agttgaatgc agaggcaaac gaagatacac ggatgttccg cgtcacagca gatatcacta
     1321 agctgggcga aggaacacat gaagttccgc taaaaattca aaatttgagc agtgctgtca
     1381 cagcaaagat cgaaccatcg actattacag taacggttga gaaaaaggta ataaaagatt
     1441 tcgatgtgga aacgttgatt ccttcaacgt tcacaccatc tggatatgaa cttgatgata
     1501 cttccgttag tcctaaaaaa gtctctatca ctacaggtga caaaacattg gcagaaatca
     1561 aacgagtagt agctgatgtg gacgcttcga tggtcacaga tgatgggatt aatagtgagg
     1621 tcccaataca ggcgctgaat gcagcagggg aagtattaag tatcgtctct gatccagtgc
     1681 aagtaacagt gagagctgat tcgatcaagc caagcaagtc ggtacgactt tatgggatac
     1741 agcaaggaac accagctgcc ggggtagaaa gctatgattt cagtttttca aaactggaag
     1801 cagaagtaag cggctctagc gatttgcttg catcgatcgg tgacagtatt gctgtaccga
     1861 tcaatgtctc aggtatcaat catcgaacaa ctcgtaaaat tgaaatacca gtagaagagg
     1921 ggttaagtat caagcccgaa tcagttagtg tggagattac acccgtgttg caaacgtcaa
     1981 gcagtagctc tgcttcgacg agcagtcgga cacaagaaaa tacaagtacg agtagaagta
     2041 tttcatctgc tgattcagga acggaaccat cgacaacaac catttcttcc agttcggaat
     2101 ctgggacaag cagctcgacc acaagtacag aagaaacatc agaaacaagc gaatcaacac
     2161 aaatgagcag aacaaattcc tcagaaaatt aagttaaagg agtaatttta caactatggg
     2221 taaatatttt ggaacagacg gcgtacgcgg tgaagccaac aaggaactta cgccagaact
     2281 tgcatttaag ctgggacggt gcggaggtta tgtcctaagc cagcatgaag aagaaggaaa
     2341 acgcccacgc gtgttagtag ggcgggatac aagaatctca ggtcaaatgt tagaagaagc
     2401 gctgatttca ggacttttat cggtgggtat cgaagtgttc cagctaggtg tgatctctac
     2461 accaggtgtt gcttatctga cacgtttgca aaaagcaagc gcaggggtca tgatttctgc
     2521 ttctcataat ccagcacaag ataatggaat caaatttttc ggtgcagatg gttttaaact
     2581 agtagatgac caagagctag aaattgaagc attattagat gctgaagtgg atgaattgcc
     2641 tcgtccatca gctgaaggat tgggaactgt agaagaattt ccagaaggct tattgaaata
     2701 ttctcaattc cttgtacaaa cgatcaacgg tgacctttcc ggtttgacca tttgtgtcga
     2761 tgcagcaaac ggagcaactt caacaagtgt taatcgtttg tttgccgatc ttgaaacaga
     2821 tttttataca atgggcacaa atccagacgg gttaaatatc aatgacggcg taggctctac
     2881 ccatccagaa cgtttagctg agatggtcgt tgaaaaagga gcagatgctg gactagcttt
     2941 cgatggcgat ggcgatcgta ttattgcggt ggatgaactt ggaaacattg tcgacggtga
     3001 taaaatcatg tttatctgtg ctaaatactt ggcagaaaaa aatcgattga aaaaagatac
     3061 gatcgttacg actgtcatga gtaacttagg attccacaaa gcggtggaag gaatcggttt
     3121 gaaagatgtc gttacacaag ttggtgatcg ttacgttgta gaagaaatgc gcaaaaacga
     3181 ttataacttt ggcggcgagc agtctgggca catgattttc ttagatttta acacaactgg
     3241 agatgggatg ctttcaggaa ttcagttact gaatatcatg aaacagacag gtaagaaatt
     3301 atcagagctt gcagcagagg taacaatcta tccgcaaaaa ttagtgaata tccgcgtaag
     3361 tgataaaaac ggtgcgatgg atgttccggc aatcaaaaaa gtgattgaag aggcagaagc
     3421 tgaaatggga tcagaaggaa gaatcctagt tcgtccttcc ggtacagaac cattacttcg
     3481 agtgatggca gaagcgccta ctgatgagaa agttaattac tatgtcgata aaatcgcggc
     3541 agtagtcaaa gaagaaatcg gactggatta agagatattt tcataaaaaa taaaagaaca
     3601 aggaccagtg tcagatgtct gacactggtc cttgttcttt tattattcta ctgtcactga
     3661 tttcgctaaa ttacgtggtt tgtcaacgtc gaatccccgt aatagcgttg cgtaatatgc
     3721 aagtaattga gtcggtacga ctgtggttag agtagttaga taagggtgga cagttggtag
     3781 caccagctgg tcactttcct tttctaatcc ctctgagaca ataactagtg ttttagcgcc
     3841 acggctttcg acttctttca ggtttcctcg agtatgcgca ccagtcactt cttcactgat
     3901 gattccgatc actggtgttc cttcttcgat caaagcaatc gttccatgtt tcaattcgcc
     3961 agcggcaaaa ccttctgcct ggatataact gatttctttc aatttcaaag cagcttcaag
     4021 agaaacgttg tagtcagcac cacgtccaat gtaaaaggca tttcttgtat cgctgagata
     4081 tacttctgcc aactcttcta aataatcttt ttgggcgatg accgattcca tcgcattagc
     4141 aactaaactc aattcatgag tgatatcgaa tacaagagaa gtttcaaatt cttttttatc
     4201 accgatagct ttggctaata aagttagtac agctatttgt gcagtataag ctttcgttga
     4261 tgctactgcg atttcgggtc cagcatgcaa tagcagtgta aaggatgctt cccgcgaaag
     4321 agtagaacca gcaacatttg taatcgtaag tgaagggtag ccgagacggt tgatttttac
     4381 cagcacttgg cggctgtctg ctgtttcgcc actttgactc aagaaaataa agaatggttt
     4441 ttttgaaaga agaggtgtgt tatagccaaa ttcactggct aaatgaacct caacaggaat
     4501 ctgtgtcaat ttttccaaag tctgtttacc tgctaacccc gcatggtaac tagttccgca
     4561 agcaacgata tagattcgat cgctatcaga caatgtatcg accagctttt gatcaagaga
     4621 gacacgattg tcttctgtta cgtatttttg agcgatacga cgcatgataa taggctgttc
     4681 gtcgatttct ttcagcatat aaaaaggata ggtacctttc tcgatatctg aagcatctac
     4741 ttgtgcttcg aatgaagaac gttctacgat gtcacctgca aaattttgaa taataacttt
     4801 ttctgatgtg acggtaacca tctcttcatc ttcaatctca acgaattcct tcgtatgagc
     4861 aagcatagcc attgcatcgc tggcaatcac attgaatcca tcgccgagtc cgattaatag
     4921 tggactttta tttttagcga cgtaaataac atcaggtgct gtccgatcaa tcaaagcgaa
     4981 agcgtaagaa cctttgatga cccgcaaagc ttttaaaaac gcctcttgcg ttgtagtatc
     5041 cttcgcgaaa gtttcaatta gatgagcgat aatctcagta tcggtctcac caataaagtg
     5101 gtcatttgct aaataagcaa ttttaagttc ttcaaagttt tcgattacgc cgttgtgaac
     5161 gagcacgaag cgtccacttt gggaagtatg tggatgggcg ttttcttcag aaggttgccc
     5221 gtgagttgcc catcgtgtat gaccaatacc tatatttcct gtagtatcaa tatttatttt
     5281 gtttttcaaa ttttgaattc tgccttgtgc tttgaccaga taatcagttg tgccatctga
     5341 gacaaagata ccagctgaat cataaccacg gtattctaat ttttctagac cgtcaatcaa
     5401 tcctggtgtt acatttttta atccaatcat tccaacaatt ccgcacataa aaaacatcct
     5461 actttctata atgaattcgt atgaatgctt ccagctgcag gaaaatcctt ttgttttatc
     5521 tttgcagaca ttttttgtgg attttctgta gagtgcagtc cctagggcca tccgccgaat
     5581 ctttcgataa accctctcct cgtcatctgt ttacacagac ctggcgctat cctaaaaggt
     5641 ataactatcc tcctttttgt tttttatctg gaaacattta aaatcataac cttttgcgaa
     5701 tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta tatatcaaat tctttgtcaa
     5761 atgtcctttt tatgtcaaaa aataatgggt ttttatctat catctttttc aggagttcgg
     5821 tacaatagaa agtaatcgga tagaggagga gtaaaaatgg attcaaaaat tgagatcatg
     5881 acactgggaa tgttaaaaaa acaattgtcc aaattcgaag catcagctgg tgtatcagat
     5941 gatacgaaaa tatttttaga tactggctgg gatagcatcc aagaaatcgc acctgatgcc
     6001 ttggaagttg ctcaagctcg tgagtttacg gtagaagatg aattaacaaa agagtcgttt
     6061 tccgggtatg cgcgggaaga aaaagcagag cggttcgaca cttctgagca gtcagaaact
     6121 gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa tgaataaaag acgttgggca
     6181 gcagttctga ttgctgcagg attatttgtg gtttcgttag tttcagctag tttgactaag
     6241 cctcaggaaa acgatcaaat tgagggattg aacagttggt tatatgggga tgaagaacta
     6301 gctccaaatg tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt atcagtagat
     6361 ggaacgattg ctgcgggagg gtcttcaggt ttatttgctt ctgaaggtta caatcataaa
     6421 aattttatgg aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg tatcttttta
     6481 gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa gaaattggat
     6541 acgattcgta aagaacatga tatcccaatg tatgtcagca tgaaaaacat ggcagctagc
     6601 ggtggctact atatttctgc tcaagcagat aaaatctttg caacagaaga aacagtgacc
     6661 ggctcaattg gtgtgatcat gtcaggattg aattattcag gacttctaga aaaactgggt
     6721 gtggaagata caacagttaa aagcggagcg ttgaaagata tgggttcagg aacgcgacca
     6781 gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg acagcgcata tagtcgtttt
     6841 gtagcaatcg tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa aattgctgat
     6901 ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga attgggattt
     6961 cctgaagatg ctttaaaagc tatacgaaag gaacaacagc ttgaagatgc ggaactagta
     7021 gaatattctt ccacgccatc tgggtttgct agtacatggt taggaagtaa actagcagaa
     7081 ttccagggat tgaagccttc tgagactagt cagatcttat ccgttcttga gaatctaggg
     7141 acagcggaat caccaaaagc aatgtattat tacggaggtg agtaagcatg tcagagcttt
     7201 caaatgaaaa taaggaacaa gaaaaagata agcaacagca gcctgctgta ggacctgact
     7261 ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg cccgaagaaa
     7321 tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac aagaaatgaa ccgccaaaag
     7381 caaatgattt tcctaagtat ttctatgcag gtttttttat ccgaatgttt gccttcttgg
     7441 tcgatctttt gtgtatcgga gctattacgc gtttaacact tggtattgcc aaaaatctag
     7501 gttggcttaa tttttcagat tcttatctga gtctctacgg attcacagcc ttacttattt
     7561 atctagcgta ttttatttta ttgacgaaat tcaatcatgg ccaaacgatt gggaaaatga
     7621 tctttggtat tcgagtcatc agctttgatg aagaagaact tagctggtca accgttttag
     7681 ttagagaagg tgcttgccgg ttcattctaa aatttccttt gctgatgtta gggtatcttc
     7741 cagcagtctt taatcagaaa aagcaacatc tgggcgattt tttcactaat acaagtgttg
     7801 ttacgatcaa tctgatcaaa gcgttcaacc aggaagtcaa cagctgatga gcaataaaaa
     7861 gcagtaacag ttgaaagcac cttctaaaag tcagatctaa aatctagctt ttagagggtg
     7921 cttcagttat ttttaattgt catgcttttt ttgttttgct actattgaac atgagctgaa
     7981 tatgagcaag aggaggacac tgcgtgaagc cgattgattt tccagaaaaa tatgagaatt
     8041 taatgcgagt ggcacaacaa gcactagcaa accagcaata tcagcaggca aaagaattat
     8101 ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa tagcctgctt gttttttgtc
     8161 tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc actcttgcat gaaaaacagt
     8221 atcttcaaaa tgaggagttt gctgaatttt attttgactt gttaatcagt acacaagatt
     8281 ttttatatgc tagaaaattg atagcaagta cagattttta tgaagctttc gagcagcggg
     8341 tcattgaaaa aatccaattt gcagaagaat tatccggaca aatggagagg cagaagatca
     8401 aggcactcca caataaatcg gaggaacttc cttcgttgga acctgcacgt cagcttgcgt
     8461 ctattgatga gatcgaacag cttccttatc atgagtttat ccaaacagcg agtaaactta
     8521 ttgtatcgcc agaggtccat attttagcaa gggcaaaatt actggagaca cttaggcaat
     8581 tgaacgaatg ccatcaagtt ttctatttga cgattgaaga aaagttagtc aaagtcatac
     8641 caaaagatct gccaaagccg cagcagcagg caagttacca gcaattatgt gtattttcag
     8701 atcgttacgg gaatgaagat gcgctgctta gttccgt
//
LOCUS       DACERL010000094         8522 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.099, whole genome shotgun sequence.
ACCESSION   DACERL010000094 DACERL010000000
VERSION     DACERL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.099"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            99..746
                     /locus_tag="IXF54_002488"
     CDS             99..746
                     /locus_tag="IXF54_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1405712.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IXF54_002489"
     CDS             1139..3784
                     /locus_tag="IXF54_002489"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1405713.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IXF54_002490"
     CDS             4094..5407
                     /locus_tag="IXF54_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1405714.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IXF54_002491"
     CDS             5397..6050
                     /locus_tag="IXF54_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1405715.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IXF54_002492"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IXF54_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1405716.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IXF54_002493"
     CDS             complement(6671..8023)
                     /locus_tag="IXF54_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1405717.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..>8522)
                     /locus_tag="IXF54_002494"
     CDS             complement(8089..>8522)
                     /locus_tag="IXF54_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1405718.1"
                     /translation="LEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIG
                     MTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKE
                     FFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
BASE COUNT         2729 a         1447 c         1688 g         2658 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 tt
//
LOCUS       DACERL010000095         8512 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.100, whole genome shotgun sequence.
ACCESSION   DACERL010000095 DACERL010000000
VERSION     DACERL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.100"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            474..725
                     /locus_tag="IXF54_002495"
     CDS             474..725
                     /locus_tag="IXF54_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405719.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IXF54_002496"
     CDS             759..1037
                     /locus_tag="IXF54_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405720.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IXF54_002497"
     CDS             1634..1891
                     /locus_tag="IXF54_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405721.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IXF54_002498"
     CDS             2004..2615
                     /locus_tag="IXF54_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405722.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IXF54_002499"
     CDS             2794..3351
                     /locus_tag="IXF54_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405723.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IXF54_002500"
     CDS             complement(4050..4571)
                     /locus_tag="IXF54_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1405724.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IXF54_002501"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IXF54_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1405725.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         2867 a         1374 c         1811 g         2460 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg ca
//
LOCUS       DACERL010000096         8494 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.101, whole genome shotgun sequence.
ACCESSION   DACERL010000096 DACERL010000000
VERSION     DACERL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.101"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..173)
                     /locus_tag="IXF54_002502"
     CDS             complement(<1..173)
                     /locus_tag="IXF54_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1405726.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IXF54_002503"
     CDS             complement(593..1714)
                     /locus_tag="IXF54_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1405727.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IXF54_002504"
     CDS             2042..3451
                     /locus_tag="IXF54_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1405728.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IXF54_002505"
     CDS             3448..4176
                     /locus_tag="IXF54_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1405729.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IXF54_002506"
     CDS             complement(4304..5215)
                     /locus_tag="IXF54_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1405730.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IXF54_002507"
     CDS             complement(5232..6239)
                     /locus_tag="IXF54_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1405731.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IXF54_002508"
     CDS             complement(6236..8365)
                     /locus_tag="IXF54_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1405732.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IXF54_002509"
     CDS             complement(8370..8477)
                     /locus_tag="IXF54_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405733.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACERL010000097         8359 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.102, whole genome shotgun sequence.
ACCESSION   DACERL010000097 DACERL010000000
VERSION     DACERL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.102"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..657
                     /locus_tag="IXF54_002510"
     CDS             <1..657
                     /locus_tag="IXF54_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405734.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
     gene            complement(1360..1575)
                     /locus_tag="IXF54_002511"
     CDS             complement(1360..1575)
                     /locus_tag="IXF54_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1405735.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1594..1830)
                     /locus_tag="IXF54_002512"
     CDS             complement(1594..1830)
                     /locus_tag="IXF54_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1405736.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2088..4241
                     /locus_tag="IXF54_002513"
     CDS             2088..4241
                     /locus_tag="IXF54_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ1405737.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLANIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4210..5625
                     /locus_tag="IXF54_002514"
     CDS             4210..5625
                     /locus_tag="IXF54_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ1405738.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5658..6692
                     /gene="sppA"
                     /locus_tag="IXF54_002515"
     CDS             5658..6692
                     /gene="sppA"
                     /locus_tag="IXF54_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1405739.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6694..7215
                     /locus_tag="IXF54_002516"
     CDS             6694..7215
                     /locus_tag="IXF54_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1405740.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7212..7598
                     /locus_tag="IXF54_002517"
     CDS             7212..7598
                     /locus_tag="IXF54_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1405741.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8094..8166)
                     /locus_tag="IXF54_002518"
     tRNA            complement(8094..8166)
                     /locus_tag="IXF54_002518"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8131..8133),aa:Lys,seq:ctt)
BASE COUNT         2954 a         1238 c         1491 g         2676 t
ORIGIN      
        1 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc
       61 cgaccaaagc aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata
      121 gaattagcag aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact
      181 catgatgaga tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca
      241 aaagatcaaa cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa
      301 gaaaggatag atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct
      361 caaacattta cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat
      421 agattatcat tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt
      481 gaaaagaaat atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac
      541 gttaaagaaa tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac
      601 tttactttag ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg
      661 ttttttcttt agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt
      721 tttctgaatc ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac
      781 agcaatatta gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga
      841 atagttcttt tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt
      901 ggaagatgcg aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta
      961 gttaaaaaat ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata
     1021 attactttcc tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca
     1081 agtacattac caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca
     1141 gcgctgcaag tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct
     1201 gactttaata aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt
     1261 aaaaagttct gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc
     1321 gacacaaacc aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct
     1381 gaatccagca cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc
     1441 ggctgaacaa agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac
     1501 ggcactccca ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt
     1561 gtttttaata tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc
     1621 tgcagcacct ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca
     1681 aattgctgtt cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg
     1741 ccccaatttt ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt
     1801 agttccttta cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct
     1861 actgtaacat acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc
     1921 ttgaatagta aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga
     1981 actgttttca aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat
     2041 tgagaatttt tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat
     2101 ttccttttgt gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt
     2161 taaaatattt taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt
     2221 tagagggtac ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta
     2281 tggctattca agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg
     2341 cacatatatt ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa
     2401 acgtactatg gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc
     2461 aaaaagaatg gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta
     2521 aagaaaaagt agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag
     2581 tattccacat cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt
     2641 attttcaagg tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa
     2701 tttccattgg cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt
     2761 atcttcttat tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac
     2821 atgtgttaac tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc
     2881 gttttcttga tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt
     2941 tagatattgg tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt
     3001 ttttaatcac tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa
     3061 gctatgaaaa atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca
     3121 tagaaagtct taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata
     3181 accgtataga ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg
     3241 ctaatattca acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat
     3301 ggctaggttc ttattttgtt atggataata gaattagtct aggtcaactt atcacataca
     3361 atgctttact agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa
     3421 tgcaaacggc tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa
     3481 aatcgaacca gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg
     3541 taaaaaacgt ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta
     3601 caattccttc gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc
     3661 ttgcaaagtt atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca
     3721 ttaatcactt agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag
     3781 agtcattttt ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac
     3841 caagtttcga acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc
     3901 aacagccatt acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc
     3961 aacgacaacg attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag
     4021 atgaagcaac tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca
     4081 atttaaaaga aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg
     4141 atcaaatttt agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc
     4201 gttttgccga tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg
     4261 cagaataata attggttcga acattctagt atctatacta aacaacatca tagcttctac
     4321 cgttgggtgt tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt
     4381 gctaaaaaag aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag
     4441 ttacaagtgc cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt
     4501 cataaggacg atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag
     4561 atagaacaag aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa
     4621 agtataagta aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa
     4681 ttaaagagca tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta
     4741 aatgatcaaa agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt
     4801 gagagttcag atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat
     4861 caatcattaa aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa
     4921 ctaaataatg tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata
     4981 aatgaacaaa ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa
     5041 ttagttaaac caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa
     5101 gagctagaac aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa
     5161 gagaagaacc agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa
     5221 gctcctgtat ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc
     5281 aaaggaacag tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca
     5341 gcacttttac ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa
     5401 ttggatcaaa aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa
     5461 aattctactg ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact
     5521 caatcgtttc cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa
     5581 acatattggc aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga
     5641 atataggagg agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat
     5701 tattgttact atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa
     5761 atacaaccct aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa
     5821 cgttagaaga aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg
     5881 cagatacagg tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa
     5941 cacagctaaa gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt
     6001 cacctggggg aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa
     6061 agctagatat tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca
     6121 tttctgctgg ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg
     6181 tcattatttc tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga
     6241 cgtataaaag tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag
     6301 agaataaagt tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag
     6361 caaaaggaag aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg
     6421 atggtaacca agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat
     6481 tagattccct aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg
     6541 acacaactgg ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa
     6601 aggcaactac atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc
     6661 caaaaccaat gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact
     6721 ttgcctaatt atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta
     6781 atttgtatta gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg
     6841 gaatctgaca aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc
     6901 tattttgtac ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg
     6961 ataagagtag tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag
     7021 tgtatatgta ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct
     7081 ttcacgccca agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt
     7141 cgttcattag aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg
     7201 gagagaatta aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa
     7261 cagggatttt atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt
     7321 caactacttt aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa
     7381 gcgggaatgt tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa
     7441 taggtttttt agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt
     7501 tatctattat tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta
     7561 ttattgcggg attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt
     7621 gaaacgatta atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg
     7681 gaagacatgt agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa
     7741 gacactctct gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt
     7801 ctattaaata ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt
     7861 agagagttca ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac
     7921 tttactgttc ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt
     7981 tgagttaaaa ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt
     8041 tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg gtttggcacc caatgggccg
     8101 tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa
     8161 cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac
     8221 atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat agattattca
     8281 catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc
     8341 taacactttt ttcattttt
//
LOCUS       DACERL010000098         8356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.103, whole genome shotgun sequence.
ACCESSION   DACERL010000098 DACERL010000000
VERSION     DACERL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.103"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..626)
                     /locus_tag="IXF54_002519"
     CDS             complement(<1..626)
                     /locus_tag="IXF54_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ1405742.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            complement(849..1880)
                     /locus_tag="IXF54_002520"
     CDS             complement(849..1880)
                     /locus_tag="IXF54_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405743.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1887..2717)
                     /locus_tag="IXF54_002521"
     CDS             complement(1887..2717)
                     /locus_tag="IXF54_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405744.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(2714..3520)
                     /locus_tag="IXF54_002522"
     CDS             complement(2714..3520)
                     /locus_tag="IXF54_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1405745.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DILSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(3526..4350)
                     /locus_tag="IXF54_002523"
     CDS             complement(3526..4350)
                     /locus_tag="IXF54_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1405746.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4340..5440)
                     /locus_tag="IXF54_002524"
     CDS             complement(4340..5440)
                     /locus_tag="IXF54_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1405747.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5618..6403
                     /locus_tag="IXF54_002525"
     CDS             5618..6403
                     /locus_tag="IXF54_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1405748.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6436..6819
                     /locus_tag="IXF54_002526"
     CDS             6436..6819
                     /locus_tag="IXF54_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1405749.1"
                     /translation="MSNLRDYNQEAPIHCLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6895..7026
                     /locus_tag="IXF54_002527"
     CDS             6895..7026
                     /locus_tag="IXF54_002527"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1405750.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6995..7231
                     /locus_tag="IXF54_002528"
     CDS             6995..7231
                     /locus_tag="IXF54_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1405751.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7309..8280)
                     /locus_tag="IXF54_002529"
     CDS             complement(7309..8280)
                     /locus_tag="IXF54_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1405752.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2405 a         1961 c         1653 g         2337 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtgactgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaattgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaaggaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccaaaat caattctacc
      781 ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccagaa atgtttgccg
      841 actatacact agctttgttg tgcaaaatgc tgagtatcaa tttccacagg gattgctaga
      901 atatctttca gctgaatgta taaggcatcg ctaattttct cttcccgatc gttcaaccga
      961 tccactaaaa tttctttgcc ctccagcgtt tgaaaaacat accgttgaat gttgcctaaa
     1021 ataaatgatt ccttccatag aaccggaatc ttaaccccct gaggaaccgg ttcaaattga
     1081 atcagctccg gtcttaaata ataaatgcag cgattgccct tagcggaaaa ctcgtgggta
     1141 tactgattca gctcatacca ttcgaaacga ttatagttgc cgataaattc cgcaacaaat
     1201 cggctctgag gattctgata caattccttc gaggtggaaa cctgagccac ccgcccagct
     1261 tccatcacaa aaatccgatc actgattcgc atcgcctctt cttggtcatg agtcacaaaa
     1321 atcatcgtca tccctagcga ttgctggata gcgcgcaaat cccgctgcag ctgttttcta
     1381 attcgggcat ccaaagcact taatggctca tccagcagca ggactttcgg ttcagtgacc
     1441 aatgagcgag ccagtgccac ccgttgtcgt tgaccgccag aaagaaactg gacattttta
     1501 tctgcctgct ccgtcatatc caccaatgat agcatctcaa aaaccttttc ctgaatcacc
     1561 tcaggactca gcttctttac cttcaaacca aaggctacat tttcaaaaac agtcatagtt
     1621 ggaaacaatg catactgttg aaaaacaaag ccgatattgc gatccttcgt aggcttatcg
     1681 gcaatattct cattgtccag cacaatctca ccgctctttg gttgttccaa tcccgtgata
     1741 cagcgcagca acgtagactt tccgcacccg ctgggaccta gtaaagtaac cagctcaccc
     1801 ttttcaatct gaatttcatc aatagctaaa atctgttttt catcaaaaaa cagattgaga
     1861 tcatgtattt ctaaaaacat tttccgtcac aactccttta agggcttctt tttcgactgc
     1921 ttctcttccc tcgtctgctt aagggacagc tgttgctttt gtcgataagt cagcataaag
     1981 gtagttaaag caattcccac cataaacagc aaatacaaaa ccatcacagc gctagccaaa
     2041 tggccgtttt cgttcatccg ccgcagcata tagatgcgta ccgtctgaaa tcgaccgcca
     2101 atcaacaggt tcgtcagcat gtactcccca aaaaccgaag aaaaaatcat cagagaaact
     2161 agggtcacac cgatacggat gtttggaaac agcacctgga taataatcgt agacatcggg
     2221 gcgcccaagg tttctgctgc atccactaat tccttcagat taattaagcg catctggttg
     2281 ttaagactaa gatagacaat cggcaacgcc gtaataaaca atgcacctac caacactaca
     2341 aacattggga taccagtttt agcatacgtt cgaagcaaag ccgttaccaa aatcactcct
     2401 ggtaaagcat acggcagcag gagcagcttt tccagccaac gattcaaacg agggaaatac
     2461 aaatgaatcc aaataatggt gggaatcatc aacaacagaa tcgtcaccaa gaccacacct
     2521 gctaataaaa gtgagcgacc caccgcagca ataaattccc tgtccgtcag cagctgttgg
     2581 aaccattgca aggtaaaatc actaggtaaa agcgattttg accatttagt ggagctggca
     2641 tacaaaatcg ttccaataat cggcagcaag taatacagga caaagcagaa caaaatcggt
     2701 tttattaact ttttcacgtc tgcaccacct tgcctttctt ttgaataatc aacattgccg
     2761 ttaccagcag gacacctaaa gcgactgcca acgcactgcc taaattaggc ttggcgaaca
     2821 catctcccga cactaaagcc gcaattcgaa tcgttaaaag atttacatta ctgctggtca
     2881 gggcatacgc tgtttcatag gtccccatgc cattagcaaa taagatgaca aaggtgctgg
     2941 caatcgatgg tcgcaaaaaa ggcaaagcca ccttgaacca agcatacgct gatgaagcgc
     3001 ccagcaattg tgcggactcc agccattcct ttttcacttc cttcatgctg ggatacaaaa
     3061 acaagacccc taacggaatt tggaaatagg tatacgtgat ggtcaatccc caccaagaat
     3121 acaaattgaa gtccgctaga aatggcaaaa tcattttcag cacacctaca ttccccaaca
     3181 aaataataaa agagaatgcc agcggaatcc cagcaaaatt agctgccaaa ttggaaacaa
     3241 aggtgatttt ctcctgcaat ttagtcgata gattcattaa gcataaacat aaaataaacg
     3301 tcccgattaa cccaatcaca ctggagctga cagcaatcag tacgctgtta acaaaggcct
     3361 gtgtgtaata tttgttgctt aaaatatcac tgtaattggc caatgtccac tggttagtta
     3421 aatcactgtg caagctgcac agaaccataa aaagcagcgg cagcagtaaa aatgcacaga
     3481 agataaagga ggcaggaacc agaaccaagc gttttttcat ctctttcatc ccctcatcaa
     3541 ttcttgaatg tcctgcattt tctctgacgg tgcaattcct aataaccagc aaaacaatgg
     3601 agccgccagc agctgtggta cagtttgttc cttcagcttt tggcgtttat ttgaaatcaa
     3661 ataaaaagga acttcacgat gtgcagccaa gctgccgcca tgcagtccgt gcgcatccat
     3721 cccgtgatcc gcagtcacca cgacttgata acctttttgc agccattcag gaaggtaaat
     3781 agccaataat ctgtccgcct gattaaccgc tgctgcatat tcatgagaat cggcggtaaa
     3841 tttgtgaccg acatcatcaa tattcatcga atgtacgacc aaaaagtctg gctgatactg
     3901 attcactaaa taatgggcat ccgcaaataa atgcgaatca gggtaagtgt cctcactata
     3961 aaagatgcca tgctgaatca gtcgctcctc ctgatgctgg atgcgatcaa ccaaacgatt
     4021 atagggggct tgattgtaca actcgctgta ccagtggtaa gcggcggctc ctgtttttcc
     4081 gccagcttcc ttaactaatt caaacagcga ccactctttg gatcgctgag catggtaatt
     4141 actatagatg ccattttcat aggctggaac gccggtcatc agtacttcgt ataacggacg
     4201 tgaattgctg ggcagctcac tttttacctt caataagctt gcttgctggt gctcaactaa
     4261 atgattcatg aacccaagct gggcgacggc tgtgtcgtag cgacagccgt ctagtataac
     4321 tacaatcagt ttgtcaagtc tactttgcat aggtcagtac ctccgcctgc caaagctctg
     4381 gaagctctac agaggtcttg ttccaagctt cattgtcttc aactggcacc gccgcttcat
     4441 aagcatcctc cggcagtaat ttttcagcta cttctttagg cagctctacc ttgcgaatag
     4501 gtcgcgcata gccctttgcc aaattgattt gcccttcatc agataaaata tattcccgtg
     4561 ctagctttgc cgcattggga tgaggggcat ttttattgat taaggttgta tacccgctaa
     4621 tgatggagcc gtctgttggc acagttacct caaaacgctc ggagtcaatt tggtcacgat
     4681 aatttagtcc attgaagtcc caaacaactg ccacatcgat ttcacctttt tcaagatttg
     4741 ctacgctggc atcaacggtg gacaaacggc ctttttctgc catttttttg tagtaattca
     4801 gacctggctg aatatccttt tcgtcacctc cgttggcaat cgccgcagcc aatacagcaa
     4861 attgtgcttg gttggcaatc gtcacatccc ccacgctaac ttgatagtca ccctcttgca
     4921 gctccttcca gctagtcggt gcttgcttca cattctcttt atcggtgata aaggaaatgg
     4981 ttcccgtata gcttaacaac caatccccat catcatcctt tgcccaatcc ggaatttcct
     5041 cccaatagtt ggtcttataa ggtaaggtta actctttgct ttcagccaac ggaccgaagc
     5101 tgataccgac atcaccaata tcggcggttc cattttttcc ttctgattca aacttagcca
     5161 attcttctgc actggacata tccgtatcca cgtgacttaa gccgtattct ttttcaaggt
     5221 cgttccaagt gcctacccaa tttgcccaag tatccggcat tcccaagctg gcaatctctc
     5281 cttcttcctt ggctttttct ataatcgtat ctaaagagtc gtctgccgca gccgattgct
     5341 tcgttgcttc acctgagccg cagcctgtca gcaggatact tactgttgct gcacaaaata
     5401 aaccgatagt catgttacga attttttcat tcattttcat tcattcttct cccctttcga
     5461 taattccatt ctaacaaagc tttgtaatga gagaatcaat ttcttgaaaa ggtttcatga
     5521 acaatgtaaa ggttgtatat ggtttgtaaa tttcgacgaa ttcccgttaa ccttctaaag
     5581 cttttgcggt atgatagtag aaaaaaggag gatccctatg cagtattatg atcagcatct
     5641 ccacacgtat ttttcctttg attcagagga aaaatttgaa aactatttag cctatcagcc
     5701 ggagtttttc gtttctacgg atcactttga tttaaacaat ccatctaccg gaaaagatga
     5761 cattccggat tatccagcct atataaacaa actggactct ttaaaagaaa cctattccac
     5821 caaatttctg cgaggtatcg agattggcgt tgttccagga caagaagagc ggatcgtgga
     5881 ttacctcgct cagcatcctt atgatttaaa gctggtaagt attcatcaaa atggccgctt
     5941 tgattatatg gacgatgtgg ttttaaccaa agatcccatt caaactacca aggaatattt
     6001 tgatcagatg ttgcaggtct tgacggattt tccagaagga gatattttga ctcactttga
     6061 ttatggatta cgccgcttca ccttcacagc ggaagaattc gcggagcatt ttgaggctca
     6121 gctgctaacg attttcaaaa aggttgttga acgagaattg gcgatggagt taaatgccaa
     6181 gagcttcttg aaatacggca acgaagacct ttaccgttat gccgttcctt tgtatcaaag
     6241 tgtcggcgga aagctcttta ccttaggctc ggatgctcat gttgccgaag attatcagct
     6301 gggctttaaa aaaatgagtc agctgttggc agactgtcag gtagaaaagt tgctagtcat
     6361 tcaaggaaaa gaacgccgcc tgacgccgct gccgattttg tgattcctat ataatagaag
     6421 aaagtggtga aaaaaatgag caacctgcgg gactacaatc aagaggcacc catccattgt
     6481 ttaattgcgc gacattggga tgcgttgaaa attgaggcgg tttgccgatc cttgttagca
     6541 gccgttccaa aacaacaact ggaaaatttt ttggtggctg acagcttgca gcgggaaaaa
     6601 gttcaggctt attttgcagc ctttaaagat cagcctttgg aatatttgca tgcgcagttt
     6661 catctttttt atcaagtagc cgccccagat gactacaacg atttacgagg acagctgcaa
     6721 ttgacctttc aagcggatga aaccgcctat accgttttat tgggaatggc tcgccttggt
     6781 gatcaggcga aggtcgagtg gcggattttc gatatttgat ggcaggatca aggcgtttca
     6841 gttgcaaacc ttcctctctt tcaattagaa aaaccgcagc aggaagaagt ttgtgtgctg
     6901 gtcaccgatg ctggtgaaat tcgtctccgc ttatttcctg accaagctcc cctggctgta
     6961 gccaactggc gctccctagc tgagcaaggc gtttatgaca acacaccttt cggctcgtgt
     7021 cattaaggaa tttgttattc aaggtggcgc tttggatggt tctggtgatg aagctgtttc
     7081 cagctatgag ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt ttaatggcgc
     7141 cttggcctta ggggaccacg gcccccacac caacggcaac cagtttttta ttgtgcaaaa
     7201 cacaaaggcg caggctgacc tgctaatgta aagcaagcat acttggagct tggaggacta
     7261 ccggagctgg atggtcgtta tactgttttt ggataagtat accaagggtt agaaatcgta
     7321 gcagcgatcg cagctcagcc gacaaacagt gaagatgtac cgattgagcc agtgttcatt
     7381 cgacaaatta acttcgaaaa aacatcgtaa aaagacactc acagacttca gcaaaagctc
     7441 tattcttgag ctggctgcag ctagtgagtg tctttcattt atccataaga taaagcttgg
     7501 attcaataaa taaataaatg ttttggttaa agctgtgacc agcaaatgag gaaccgtgcg
     7561 agccagttga tctgccaatg caaacatttc tttaggtgaa ggcgctggcg tgtctgccaa
     7621 cggatactcc agtcccaatg cttggtaacc gtgcatacca tagcagtggt acggtagtaa
     7681 ttctaacggt acgaaccgat caagatctgc cacaaaacaa gcaatcgtca gcagctcggt
     7741 caggttcata ttgatacctg gaaccacagg aactcgaatt cgcacttcgc agttaggata
     7801 ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg ttatctaagc cggtccattg
     7861 tttgtggagc ttgctggtaa acatttttac atccacaaac aatgtatcca catagggagc
     7921 taccttttgg atttcattat aagcaccaca aaagctggtt tccagtacgg tattgattcc
     7981 aatgtatttc gatttttgca aaatttcttt agcaaaatcc gcctgaacca gtgcctcgcc
     8041 tccgctaatg gtcactccgc catccgagtg aaagtaaaaa acagcatcct tctcaatctc
     8101 atccattacc tcttcagcgg tcatggtctg gccaaatcct ttttcaatct gcttgctttg
     8161 tgattcggga atcgaacacc aggcacagcg taatgggcag ccctttaaat aaacaacggt
     8221 ccgtaaacca gcaccatcat gtagagaacc ctgttctatt cttaatactg ttccttccat
     8281 atagattttt gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa cttaccattt
     8341 aatcataaaa ttagct
//
LOCUS       DACERL010000099         8293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.104, whole genome shotgun sequence.
ACCESSION   DACERL010000099 DACERL010000000
VERSION     DACERL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.104"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(48..1514)
                     /locus_tag="IXF54_002530"
     CDS             complement(48..1514)
                     /locus_tag="IXF54_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1405753.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IXF54_002531"
     CDS             1901..2497
                     /locus_tag="IXF54_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1405754.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IXF54_002532"
     CDS             complement(2555..3037)
                     /locus_tag="IXF54_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405755.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IXF54_002533"
     CDS             complement(3143..3340)
                     /locus_tag="IXF54_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405756.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IXF54_002534"
     CDS             3635..4393
                     /locus_tag="IXF54_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1405757.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IXF54_002535"
     CDS             4453..4881
                     /locus_tag="IXF54_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405758.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IXF54_002536"
     tRNA            4927..4999
                     /locus_tag="IXF54_002536"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IXF54_002537"
     CDS             complement(5149..5520)
                     /locus_tag="IXF54_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1405759.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IXF54_002538"
     CDS             5735..5920
                     /locus_tag="IXF54_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405760.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IXF54_002539"
     CDS             complement(6003..6191)
                     /locus_tag="IXF54_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405761.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IXF54_002540"
     CDS             complement(6215..6478)
                     /locus_tag="IXF54_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405762.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IXF54_002541"
     CDS             6636..6893
                     /locus_tag="IXF54_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1405763.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IXF54_002542"
     CDS             complement(6964..7155)
                     /locus_tag="IXF54_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405764.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IXF54_002543"
     CDS             complement(7296..7616)
                     /locus_tag="IXF54_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405765.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IXF54_002544"
     CDS             complement(7639..8073)
                     /locus_tag="IXF54_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1405766.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2630 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acg
//
LOCUS       DACERL010000100         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.105, whole genome shotgun sequence.
ACCESSION   DACERL010000100 DACERL010000000
VERSION     DACERL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.105"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXF54_002545"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXF54_002545"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1405767.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXF54_002546"
     CDS             complement(2032..3273)
                     /locus_tag="IXF54_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1405768.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXF54_002547"
     CDS             complement(3291..4214)
                     /locus_tag="IXF54_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1405769.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXF54_002548"
     CDS             complement(4435..6927)
                     /locus_tag="IXF54_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1405770.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXF54_002549"
     CDS             complement(6934..7401)
                     /locus_tag="IXF54_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1405771.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXF54_002550"
     tRNA            complement(7537..7610)
                     /locus_tag="IXF54_002550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IXF54_002551"
     tRNA            complement(7668..7741)
                     /locus_tag="IXF54_002551"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2162 a         1691 c         1246 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acaaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACERL010000101         7707 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.106, whole genome shotgun sequence.
ACCESSION   DACERL010000101 DACERL010000000
VERSION     DACERL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.106"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            151..2094
                     /locus_tag="IXF54_002552"
     CDS             151..2094
                     /locus_tag="IXF54_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1405772.1"
                     /translation="MLSKRESQLIELLLEEENFQPASFFSEKLNVSNKTIYQDIQKLG
                     QFLNRFELKIEKKPRMGIYLIGTSTKKNDAKIFITSKEAMSTKVKYSSNYRRVYLFAN
                     LIFKTDKKRYEYFEKKFFVSIATLRKDYEEIIKYCRLAGIRITSNHVIDTQKTPEELI
                     QRTFVNYFEKNIGVGNEKCQYHFVFDQAMVKFVDDFVKNFYQQSKQQPTNYIVHSLKL
                     SLLTLIGRVKHGYHSQTNKNPLFVEIKKMELYMVALDFSNGVKDELNCSFTINDLYFI
                     CSLFMGHGVRPFVNLATHSQQIMETTKQFIQDMSTLVEKDLGQDSQLLQSLFAHIEPM
                     VYRLQMGITIKNPLKEDIIRQYSTMYTLATYCIPKLEKAMGIRVTDDEITFLTIHFQL
                     AFEKIVNTKHVFLICPTGLGTSQLLFQRIRHSSPSTVVYEVIDVQKLQDYNLDSVDLI
                     ISTVKLENEYQTPVIYVRPLPTKEEIALINRHLLTLNQQDNTFLPSIAPDKTILKKYL
                     KDGWMFLNQDFDSRESVLSFITEKYKEEQLVKDNFIEVVLQREKLGATGLSSGVAIPH
                     ANPKYVVKTKISIITLKNKITWGDVDIRVVLFLAIAEEDMIQVKNMIALLYDVFVSPE
                     RIKSIASKETRNEIINEIIGKRK"
     gene            2096..2557
                     /locus_tag="IXF54_002553"
     CDS             2096..2557
                     /locus_tag="IXF54_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1405773.1"
                     /translation="MTSYFGKKIALFQSEVKSQKEALSIIATCLYQTGLVKDSFAENV
                     LKREEVFPTGLLINDVGIAIPHTDAEHVNDSQIAFMSLKTPVTFYEMGTADKEISVSL
                     IFMLALKEPHAQLEMLQKLITMFQKKEVVQKLRAVNNTEEYYQIIEKEGFV"
     gene            2581..3927
                     /locus_tag="IXF54_002554"
     CDS             2581..3927
                     /locus_tag="IXF54_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1405774.1"
                     /translation="MLDLLQKFVDLGSIVVLPILIFVFGLILGTKPSKAFISALTVGV
                     GFVGLNLVIDLLTNSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTVLGSLAIPVA
                     IVVNVLLIIVGLTKTLNVDIWNIWHAAFVASLVYAVTNDFAMGMLATVIYTMMILLFG
                     DILGPIINKFYGFPNITFPHGTSAPGFVFALPLNWLFDRIPGLKDWHADPETIQKRFG
                     IFGDSTVMGFLIGLVMGILAGYDVAGIGQLAVKTGAVMVLMPKMVALLMEGLTPISES
                     ANSFVQKRFPGRELYIGMDAALSVGHPAVLSSSLILVPITILLAVILPGNTTLPFGDL
                     ATIPFLICLMAAVFGGNIIRTILGGTIYMVTILYITSWVAPFLTKAAQGANFDLQGHS
                     SVTAMAEGGLWTTLLYVGGAKLFGYFALVGIGLIITGLLFYVNRILPKRAAKQTEA"
     gene            3950..4234
                     /locus_tag="IXF54_002555"
     CDS             3950..4234
                     /locus_tag="IXF54_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1405775.1"
                     /translation="MKKLLIMCGTGVATSTVVTGKVKEWLKSKGLENEVKLYQSKVAD
                     EIGKIDDYDIVVSTTVVPDNIKDKVIMGLPLLTGIGIDGLFDEIEAKIKA"
     gene            4261..5088
                     /locus_tag="IXF54_002556"
     CDS             4261..5088
                     /locus_tag="IXF54_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAQ1405776.1"
                     /translation="MLVTTKEMFEKAQKENYAIPAANFFDLDSARTFVEVAEKMQKPL
                     ILAFAQAHIEECPLEEAALIATYLAKKASVPVALHLDHGQDEEIIKQAIELGFTSVMI
                     DASQDEIETNIRRSKAIAEYAHERGVVVEAEIGHVGTGNSYEFKETTDTIYTEVEEAA
                     RFAKETGVDSLAVSIGTAHGQYKGTPKISFERLEEIKNEVSIPLVLHGGSSSGDENLH
                     KCAVSGINKINIFTDFITAAMDVTNEEKPEDYFTLKRLANQAIADTLSHYYEVFATK"
     gene            5101..5796
                     /locus_tag="IXF54_002557"
     CDS             5101..5796
                     /locus_tag="IXF54_002557"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1405777.1"
                     /translation="MRTVRKPFFVVNPKSYLYGEQALKLAKTADQLAAAYDIDIIFTC
                     QHVDVRLIKEQTTQLIVSVQHIDGIVPGRGMGHVLPEGVVAAGAEATFLNHAERPLSV
                     SELCDAVKRAKELGLLTIVCANSLEEAKLVASLKPDVMVCEPTELIGTGQASDISYMI
                     QTNEAVRKMDPEIFVLQAAGISTVADVRKALDSGADATGGTSGIVCAKDPVDTLKAMI
                     EEVSKWKKEQSTK"
     gene            5793..6308
                     /locus_tag="IXF54_002558"
     CDS             5793..6308
                     /locus_tag="IXF54_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1405778.1"
                     /translation="MITYTEQLVLTSNGQRVTYHNITEQVKEIVAKSGIKNGICLVQS
                     PHTTCSVIFEEFMHDLDFNGDEFLQVDLNRILDRIIPRELTENTDYRYPGPKHLDFLL
                     SLDDPDYAGDAGTILNGDAHIRASFFGASENFIVKEEILMTGTVGSIYFIDFDQNRVR
                     NRKCHILVMGE"
     gene            complement(6435..7607)
                     /locus_tag="IXF54_002559"
                     /pseudo
     CDS             complement(6435..7607)
                     /locus_tag="IXF54_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
BASE COUNT         2474 a         1206 c         1604 g         2423 t
ORIGIN      
        1 taaaaaaatt gtcgtgagtt ttactgacat caaaaagtat agggtcataa taatctgttc
       61 cacattaatg tggaacagat tacgtattga tctttagtga gaaaaactct atgatataga
      121 caaggaaaac gttttctgag gtgatgatga atgttatcaa agcgcgagag tcagttaatt
      181 gaattacttt tagaggagga gaattttcaa ccagcaagtt tctttagtga aaaactcaat
      241 gtttcgaata aaacaattta tcaagatatt cagaagctgg gacagttcct gaatcgtttt
      301 gagttaaaaa ttgaaaaaaa acctcgcatg gggatttact taattggaac gtcaactaaa
      361 aaaaatgatg ccaaaatatt tatcactagt aaagaagcta tgtcaacaaa ggtaaagtat
      421 agttcaaatt atcgtagagt gtacttattc gccaatctaa tttttaaaac ggataaaaaa
      481 agatatgagt attttgaaaa aaagtttttt gtcagtattg caactttgcg taaggactat
      541 gaagaaataa tcaaatattg tcggttagct gggattagga ttacttcaaa tcatgtaata
      601 gacacacaaa aaacacctga agagttgatc cagcggacat ttgttaatta ttttgaaaaa
      661 aacatcggtg tagggaatga aaagtgtcaa tatcactttg tttttgatca agcgatggtt
      721 aagtttgtgg atgattttgt aaaaaatttt taccaacagt ctaagcagca accaacaaat
      781 tatattgtcc attcactcaa actttcgttg ttgacgttga ttggtcgagt aaagcatggc
      841 taccattcac aaacaaataa aaatcctttg tttgtcgaaa taaagaaaat ggaattatat
      901 atggtggcac tagatttttc aaatggggtc aaagatgaat tgaattgttc ctttacgata
      961 aatgatcttt actttatttg ttcgttgttt atgggacatg gagtgagacc gtttgtgaat
     1021 ctggcaaccc atagtcaaca aatcatggag accaccaaac aattcattca agatatgtcg
     1081 acactggttg agaaggattt ggggcaagat agtcagctgc ttcaatctct atttgcccat
     1141 attgagccaa tggtttatcg gttgcaaatg gggattacca ttaaaaatcc tttaaaagag
     1201 gatatcattc ggcaatacag cacaatgtat acgctagcaa cttactgtat tccaaaattg
     1261 gaaaaagcaa tggggatcag agtaacggat gatgaaatta cttttttgac tattcatttt
     1321 cagttagctt ttgaaaaaat tgtgaatacg aagcatgttt ttcttatttg tccaacgggg
     1381 ttgggaactt cgcaattatt atttcagcgt attcgtcata gttctcctag taccgtagtc
     1441 tatgaagtga ttgatgttca aaagttgcag gattacaact tagatagtgt cgatttgatt
     1501 atttcaacag tcaaattaga aaatgagtat caaacgccgg ttatttatgt tcgaccatta
     1561 ccaacgaaag aagaaattgc attgattaat cgtcatcttc ttaccttgaa tcaacaagac
     1621 aatacatttt taccatccat tgcaccggat aaaacgattt taaagaaata tttgaaggat
     1681 ggttggatgt ttttgaatca agattttgat agtagagaat ctgtactttc tttcatcact
     1741 gagaaatata aagaagaaca attagtaaag gataatttta ttgaggttgt acttcaaaga
     1801 gaaaaactag gagctaccgg actttcatca ggcgtagcca tcccacatgc aaatcctaaa
     1861 tatgtagtca aaacaaaaat ttctattatc actctaaaaa acaaaattac ttggggagat
     1921 gtggatataa gggttgtttt attcttagcg attgcagaag aagatatgat tcaagtgaaa
     1981 aatatgattg cactacttta tgatgtcttt gtctcacctg agagaataaa aagtattgca
     2041 agtaaagaaa caaggaatga gattattaac gaaattatag gaaagaggaa gtaagatgac
     2101 gagttatttt ggaaagaaaa ttgcattatt tcaatctgag gtaaagagtc agaaggaagc
     2161 tttatcaatt attgcaactt gtctatatca aacaggttta gtgaaggatt cgtttgcaga
     2221 gaatgtactg aaaagagaag aagtgtttcc tactggctta ttaatcaatg atgttgggat
     2281 tgctatacca catacggatg cagaacacgt gaatgattct caaattgcat ttatgtcatt
     2341 gaaaacacca gtaacttttt atgaaatggg aactgcagat aaggaaattt cagtcagctt
     2401 gatcttcatg ttggcgttga aagaaccaca tgcacagtta gaaatgttac agaagttaat
     2461 tacgatgttt caaaaaaaag aggtggttca aaagcttcga gctgtaaata atactgagga
     2521 gtattatcaa attattgaaa aagaaggatt cgtttaataa ccattttgag ggggagcatt
     2581 atgttagatt tattacaaaa atttgttgat ctcggatcaa tcgttgtatt gccaatctta
     2641 atttttgttt ttggtttgat tttaggaaca aaaccgagta aggcttttat ttcagcatta
     2701 acggttggtg ttggatttgt cgggttgaat ttagttatcg atttattgac caatagctta
     2761 ggaccagcag cacaagcgat ggttgaacgt tttggactaa acttaacaac cattgacgtt
     2821 ggttggccgg cagcagctgc gatttcttat ggaaccgttt taggtagttt ggcgattcca
     2881 gtagctatcg tagtcaatgt gttattaatt attgttggct taacaaaaac actgaatgtt
     2941 gatatttgga atatctggca tgcagcattt gttgcttcac ttgtctacgc agtaacgaat
     3001 gactttgcaa tgggcatgtt ggcaacggtt atttatacaa tgatgatttt attgtttggc
     3061 gatattttag gaccaatcat caataaattc tatggatttc caaatattac cttcccacac
     3121 ggaacatcag cacctgggtt tgtttttgcc ttacctttaa attggttatt cgataggatt
     3181 ccaggattga aagattggca tgcagatcca gagacgattc aaaaacggtt tggcatcttt
     3241 ggtgattcaa cagtcatggg ctttttaatt ggtttggtta tgggaatttt agctggctat
     3301 gatgttgcgg gaatcggtca attagcggtg aaaactggtg ctgtaatggt cttaatgcct
     3361 aaaatggtgg cacttttaat ggaaggctta acacctattt cagaatcagc aaattcattt
     3421 gttcaaaaac gtttcccagg aagagaatta tatatcggaa tggatgccgc attgtcagta
     3481 ggacacccag ctgtattgtc ttcttcgtta atcttagtcc caatcacgat tttattagca
     3541 gtgattttac caggtaacac aaccttgcct ttcggtgatt tggcaactat tccattctta
     3601 atttgtttga tggcagctgt ctttggtgga aacattattc gaacaatctt aggtgggacg
     3661 atctacatgg tcaccatttt atacatcact tcatgggtgg caccattttt aacaaaagca
     3721 gcacaagggg caaactttga tttacaagga cattcatcag taactgcgat ggcagaaggt
     3781 ggactgtgga caacacttct atacgtgggt ggtgcaaaac tatttggcta ctttgcccta
     3841 gtcggaattg gtctgattat tacaggctta ctattctacg tgaatcgcat tttacctaaa
     3901 agagcagcta aacaaactga agcttaatac aaaataggag agatgacaga tgaaaaaatt
     3961 attaattatg tgcggaacag gtgtggctac ttcaacagtg gtaacaggaa aagtaaaaga
     4021 atggttgaaa tcaaaaggac tcgaaaatga agtgaaacta tatcaatcaa aagttgctga
     4081 tgagattggc aaaattgatg actacgatat tgtggtcagt acgaccgtgg ttcctgacaa
     4141 cattaaagat aaagtaatta tggggcttcc tttattaacc ggaattggca ttgatgggtt
     4201 gtttgacgaa attgaagcga agattaaagc ctaaaaaata cttgaaaaga aggtaatcaa
     4261 atgttagtta caacgaaaga gatgtttgaa aaagcgcaaa aagaaaacta tgcgattccg
     4321 gcagcaaact tttttgattt agattctgca agaacctttg tagaggttgc agaaaaaatg
     4381 caaaagccat taattttagc ctttgcacaa gcgcatattg aagaatgtcc tcttgaagag
     4441 gcagcgttaa ttgcaacata tttagccaaa aaagcaagtg ttccagtagc acttcatttg
     4501 gatcatggac aagatgagga aatcatcaag caagcaatcg agcttgggtt cacttctgtg
     4561 atgatagatg cttcgcagga tgagattgaa acaaatattc gtcgttcaaa ggcgattgcg
     4621 gaatatgctc atgaaagagg cgttgtggta gaggctgaga ttggacacgt gggaacggga
     4681 aatagttatg aatttaagga aaccacggac accatttata ccgaagtgga ggaagcagca
     4741 cgttttgcga aagaaaccgg cgtcgattca ttggcagttt cgataggtac ggctcatggg
     4801 caatacaaag gaacaccgaa aatcagcttt gagcggttgg aagaaattaa aaatgaagtt
     4861 tcaattccat tagtgttgca tgggggctca tcatctggtg atgaaaatct ccacaaatgt
     4921 gctgtttcag ggatcaataa aattaatatc tttactgatt ttatcacagc agcaatggat
     4981 gtaactaacg aagaaaaacc agaggattat tttacactta aacgattagc gaaccaagcg
     5041 attgcggata cgttatcgca ttattacgaa gtatttgcaa caaaatagag gagggttctc
     5101 atgagaacag taagaaaacc attttttgtg gttaatccta aatcttattt gtatggtgaa
     5161 caggcattga aactagcaaa aacagcggat caattagctg cagcatacga catcgatatc
     5221 atttttactt gtcaacatgt tgatgttcgg ctaattaaag aacagacaac gcagttgatt
     5281 gtgagtgttc aacatatcga tgggatagtc cctggtagag ggatgggaca cgttttacct
     5341 gaaggcgttg tagcggctgg agcagaagcg accttcctta atcacgcaga acgtccttta
     5401 tcggttagtg agctgtgcga tgccgtcaag cgagcaaagg aacttggtct attgactatt
     5461 gtttgtgcaa attctttaga agaggctaag ctcgtagctt ccttgaaacc tgatgtgatg
     5521 gtttgtgagc ctacggaatt aataggtaca ggtcaagcaa gtgacatttc ttatatgatt
     5581 caaaccaatg aagctgtacg caagatggat cctgaaattt ttgttctgca ggcagcaggg
     5641 atttcaacgg tagcagatgt tcgcaaagca cttgattcgg gggctgatgc aacgggtgga
     5701 accagcggga ttgtttgtgc caaagatcct gtggacacat tgaaagcgat gattgaagaa
     5761 gtaagtaaat ggaaaaagga gcaatcgaca aaatgataac ttatactgag caacttgttt
     5821 tgacttcaaa tggacagcga gtgacgtatc ataatattac cgaacaagta aaagaaattg
     5881 tggcaaaaag tgggataaaa aatggcattt gtcttgttca gtcgccacat actacttgtt
     5941 cagttatttt tgaggaattc atgcacgatt tagattttaa tggggatgaa tttttacagg
     6001 ttgatttaaa tcggattctt gatcgaatca ttcctcgaga attaacggaa aataccgatt
     6061 atcgttatcc tggtccgaag catcttgatt tcttgctgtc cttagatgat ccagattatg
     6121 ctggtgatgc tggaacgatt ttaaatggag atgcacatat ccgagcatca ttcttcggag
     6181 caagtgaaaa tttcattgtc aaagaggaaa tactaatgac aggaactgtc ggctccattt
     6241 atttcattga ttttgatcaa aaccgtgtta gaaatcgaaa atgccatatt ttagtaatgg
     6301 gtgaatagaa aagtaataag aaacaaacat ttgggaaact aattgaatga tcacacctat
     6361 aaaaattaat acgaaagata atagagtcaa gtccagactc ctgtgtaaaa tgctatacaa
     6421 tgtttttacc atttctactt atcaaaattg atgtattttc ttgaagaata aatccattca
     6481 tcatgcaggt ccataagaac ggctccaatt aagcgattgg ctgatgtttg attggggaag
     6541 atgcgaataa tcttttctct tctgcgtact tcttgattca gtcgttcaat tagattggta
     6601 ctctttagtc gattgtggga atttccttgt acggtatatt gaaaggcgtc ttcgaatcca
     6661 tcatccaatg atgcgcaagc ttttgaatat tttggttgat cgatataatc atgaatcaat
     6721 cgatttttag cctcacgcgc taagttaata tctgtgaact taaaaattcc tttaacagct
     6781 tctctgaaag attttgaatt ttttttagga atggtggtaa agatatttct taggaagtga
     6841 acttggcatc tttgccaact tacgttggtg aaggattttc taatggcaga gactaatcct
     6901 ttgtgcgcat cagaaataac gagttccgta ccttgtaaac cgcgttcttt taggtattca
     6961 aaaaatgttg tccaggtctc ttcgctttcg ccactttgaa tcatgaagcc gataatttca
     7021 cggtcgccat ctttggttat tccaatcgct atatgacagc tttttgagag tactcgattt
     7081 tcttctcgta cttttatata gagtacatcg gtcattaagt aaggataatt tttttctgat
     7141 aataaacgat tctgccactc gttaaccata ggttctagct gttctgttaa gctagaaacg
     7201 aaggacttag agacggattt accacaaagt tcttccacaa tttttgatac tttacgagtt
     7261 gaaacgcctg atacatacat ttccaacatt gaagccatga gggctttttc gtttcgttga
     7321 taacgttcaa acactgtggg tgaaaaatgg ccatcacgtg ttctgggtac ttttaattct
     7381 agcgtgccta cacgtgtcgt aaagctgcgc taataatagc catttcgttg actttgtcgg
     7441 ttttctgttc gttcatattc ttttgcttga atatattctg ttcgttgatt ttccattagt
     7501 tgattaaata ccgttgttaa aatattttta gaaacgtcat cctttacaga atattcaata
     7561 atgctttgaa tctcttcgct tttcagtgta aaatgtactt gggtcatgta aaagtcctcc
     7621 tgggtatgtt tttgtcgtta aaaacattgt accgtaaaag gactgttata tggccttttt
     7681 acttttacac aattatacgg actttat
//
LOCUS       DACERL010000102         7597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.107, whole genome shotgun sequence.
ACCESSION   DACERL010000102 DACERL010000000
VERSION     DACERL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.107"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(136..426)
                     /locus_tag="IXF54_002560"
     CDS             complement(136..426)
                     /locus_tag="IXF54_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405779.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IXF54_002561"
     CDS             complement(544..804)
                     /locus_tag="IXF54_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405780.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IXF54_002562"
     CDS             complement(941..1354)
                     /locus_tag="IXF54_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1405781.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IXF54_002563"
     CDS             complement(1810..2295)
                     /locus_tag="IXF54_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405782.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2679)
                     /locus_tag="IXF54_002564"
     CDS             complement(2434..2679)
                     /locus_tag="IXF54_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1405783.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IXF54_002565"
     CDS             2872..3672
                     /locus_tag="IXF54_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1405784.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IXF54_002566"
     CDS             3691..5559
                     /locus_tag="IXF54_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1405785.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IXF54_002567"
     CDS             5552..6043
                     /locus_tag="IXF54_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1405786.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IXF54_002568"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IXF54_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1405787.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IXF54_002569"
     CDS             6603..>7597
                     /locus_tag="IXF54_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1405788.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2234 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACERL010000103         7487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.108, whole genome shotgun sequence.
ACCESSION   DACERL010000103 DACERL010000000
VERSION     DACERL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.108"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IXF54_002570"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IXF54_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1405789.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="IXF54_002571"
     CDS             complement(2945..3379)
                     /locus_tag="IXF54_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405790.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="IXF54_002572"
     CDS             complement(3396..4601)
                     /locus_tag="IXF54_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1405791.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="IXF54_002573"
     CDS             5295..5681
                     /locus_tag="IXF54_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1405792.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5966
                     /locus_tag="IXF54_002574"
     CDS             5775..5966
                     /locus_tag="IXF54_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405793.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6480..6812
                     /locus_tag="IXF54_002575"
     CDS             6480..6812
                     /locus_tag="IXF54_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1405794.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7108..7263
                     /locus_tag="IXF54_002576"
     CDS             7108..7263
                     /locus_tag="IXF54_002576"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1405795.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2482 a         1320 c         1082 g         2603 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     5881 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     5941 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     6001 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     6061 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     6121 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     6181 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     6241 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     6301 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     6361 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     6421 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     6481 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     6541 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     6601 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     6661 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     6721 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     6781 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     6841 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     6901 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     6961 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     7021 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     7081 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     7141 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     7201 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     7261 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     7321 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     7381 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     7441 gttgccaata ctttttcttt tttctaataa acgcaaacag acacctt
//
LOCUS       DACERL010000104         7178 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.109, whole genome shotgun sequence.
ACCESSION   DACERL010000104 DACERL010000000
VERSION     DACERL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.109"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..218)
                     /locus_tag="IXF54_002577"
     CDS             complement(<1..218)
                     /locus_tag="IXF54_002577"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1405796.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKP"
     gene            complement(329..583)
                     /locus_tag="IXF54_002578"
     CDS             complement(329..583)
                     /locus_tag="IXF54_002578"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1405797.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(776..1051)
                     /locus_tag="IXF54_002579"
     CDS             complement(776..1051)
                     /locus_tag="IXF54_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1405798.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1023..1976)
                     /locus_tag="IXF54_002580"
     CDS             complement(1023..1976)
                     /locus_tag="IXF54_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1405799.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2588..4081
                     /locus_tag="IXF54_002581"
     CDS             2588..4081
                     /locus_tag="IXF54_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1405800.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4216..4512
                     /locus_tag="IXF54_002582"
     CDS             4216..4512
                     /locus_tag="IXF54_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1405801.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4536..5495)
                     /locus_tag="IXF54_002583"
     CDS             complement(4536..5495)
                     /locus_tag="IXF54_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1405802.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5678..6529
                     /locus_tag="IXF54_002584"
     CDS             5678..6529
                     /locus_tag="IXF54_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405803.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6550..6762
                     /locus_tag="IXF54_002585"
     CDS             6550..6762
                     /locus_tag="IXF54_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405804.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2389 a         1156 c         1295 g         2338 t
ORIGIN      
        1 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
       61 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      121 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      181 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      241 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      301 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      361 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      421 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      481 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      541 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      601 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      661 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      721 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      781 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      841 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
      901 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
      961 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1021 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1081 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1141 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1201 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1261 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1321 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1381 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1441 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1501 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1561 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1621 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1681 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1741 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1801 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1861 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     1921 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     1981 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2041 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2101 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2161 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2221 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2281 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2341 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2401 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2461 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2521 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2581 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2641 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2701 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2761 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2821 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     2881 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     2941 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3001 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3061 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3121 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3181 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3241 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3301 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3361 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3421 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3481 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3541 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3601 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3661 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3721 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3781 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3841 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     3901 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     3961 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4021 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4081 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4141 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4201 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4261 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4321 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4381 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4441 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaaa aaggaagaag
     4501 tatatccagt agtagttttt atttactctt aattgtcaaa ttaagttaga caaatcatca
     4561 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aattcagtga ttttctagga
     4621 atattatttc gtttcgatgc gacagattga ataaaagatt catctactga attgaaatcc
     4681 atagatttca ataaaccatc tctacgtaac aatccgttag aattctcgtt taggcctctt
     4741 tgagacggcg ttcctggatc agcgaaataa atggcaatat cattggcatt actgatctgt
     4801 ttccaatttg aaaattcctt tccacaatca aaagtgatgg atttgaatag atttttcggt
     4861 acagattcaa accaatgatt taatttgttt tcaatatcaa tcgcttgtct accacacggt
     4921 ttcaatgtga tgataacttt tgataatcgc tcaactaagg taattacagc acttttatgt
     4981 ttcaaaccta cgatagtgtc accttcaagg tgaccaaact catttgagaa ttggctataa
     5041 tctttttcac gttcatgaat agagcgacgg aaagcttgtt ttccacgtct ttcttgatgt
     5101 ccatttggtt tacgcttgcc tttcatcggt aagtcagaag gattaaatag cccctttttg
     5161 aacatacgat aaatggtacg agcagaacaa cgaataggaa acgctgcacg accaacaatc
     5221 acatcgggtg tccatccttg aacaaccttt ctttgaatat attctgattg ttcctcaggt
     5281 aaaacaagcg ggcgtctacc acagtttgat ttgtttttct tatactgttg atagtactct
     5341 aaggctgatt ttccttgctt gaagaacagg tatactttat ggatcgtttg tcttgaacga
     5401 ttcaagaaat gcgcagtttt cgcaacagat tgatttattt tgaaataaga ctctatcatt
     5461 acaagttcat ccgttgtaag atgggtatag gtcatttgta ctcactcctt ataattcttt
     5521 ggacggaact attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac
     5581 aatcggcgta cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg
     5641 aaaacgttaa aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga
     5701 acacacctgg cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt
     5761 atggccatta ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta
     5821 ctcgattgga gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc
     5881 atcagacatt tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac
     5941 tcaattagtc atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct
     6001 attcgattta attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc
     6061 tggaagaaca acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg
     6121 acacatgtat ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa
     6181 acttattaaa cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg
     6241 ttatgagtac aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca
     6301 cacgagtatc catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga
     6361 aatgaaagca atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct
     6421 acgatatggc atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata
     6481 cattttttgg aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat
     6541 cgtttgaaaa tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc
     6601 ttaaagaagg agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa
     6661 acgggtacga tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat
     6721 tttttgttga ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat
     6781 tgattgatga atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt
     6841 tatttgtaaa gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc
     6901 tgtgcctatt acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt
     6961 gaaaggatag tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct
     7021 tttcgctatc ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat
     7081 gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac ggttctgttg caaagtttta
     7141 aatctactat caaataaggt agaataatag aaaaagat
//
LOCUS       DACERL010000105         7158 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.110, whole genome shotgun sequence.
ACCESSION   DACERL010000105 DACERL010000000
VERSION     DACERL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.110"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            24..1316
                     /locus_tag="IXF54_002586"
                     /pseudo
     CDS             24..1316
                     /locus_tag="IXF54_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1835..2569
                     /locus_tag="IXF54_002587"
     CDS             1835..2569
                     /locus_tag="IXF54_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1405805.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2585..3754
                     /locus_tag="IXF54_002588"
     CDS             2585..3754
                     /locus_tag="IXF54_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1405806.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3770..4765
                     /gene="lacD"
                     /locus_tag="IXF54_002589"
     CDS             3770..4765
                     /gene="lacD"
                     /locus_tag="IXF54_002589"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1405807.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4844..5770
                     /locus_tag="IXF54_002590"
     CDS             4844..5770
                     /locus_tag="IXF54_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1405808.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            5789..7039
                     /locus_tag="IXF54_002591"
     CDS             5789..7039
                     /locus_tag="IXF54_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405809.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2348 a         1194 c         1470 g         2146 t
ORIGIN      
        1 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gccgaaaagt
       61 tagctgaatc cattctgaat gcctatcaac ctgaatctgt cgatgacatg caagatgctt
      121 tgaaagatgt gtttgggccc ctttttgaaa aaatgcttca aggagaattg aataatcatt
      181 taggttatga tgcccattct aaagagccta aggaacacga taaccgtcga aatggctatg
      241 gaactaaaac gcttaaaacc agttttggtg aagtagctat tgatgttcct agagaccggg
      301 aagcttcctt tgaaccagag ttaattccta agagaaaacg agatgtctcc gacatcgaag
      361 ggaaggttct ttccatgtat gcacgaggaa tgagtcaatg agatattgcc gcaaccgtcg
      421 aagctattta tggctttgat atttcccatg aaatgatttc agatatcact gacgctgtcc
      481 ttcctgaatt ggaagaatgg caagcccgcc ccctagcaaa gtgttatgcc tttctattcg
      541 ttgattgtat gtatgttact ttacgagaaa attatgaagc caaagaatat gctgtacaca
      601 ccattcttgg ctatgatctc aaaggaaata aggagatttt aggattatgg ttaaatcaaa
      661 cagaatctaa aaatcgctgg atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag
      721 atgtcttttt catttcgatg gacggtgttt ctggcctcga agaaggcgca aaagcgatct
      781 ttccatcggt aattgttcag cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata
      841 ttccaagtaa ggactataag gaagtctgcc gagatatgaa aaagttctac ggtgcttctt
      901 ctctaaacgc tgcacatgct gcttttgaca gttttcaaaa tcggtggtct cattattctg
      961 gtgctgtaga tgtctggaaa cggaactttg cacatgttga acaactattt gattatggta
     1021 gtgccattcg aaaaattatg tacactacca atgcggttga aagtgtccac tccagcttca
     1081 gaaaagtcac aaaaaaagga gcgttctcta atgagaatgc actcctaaaa ctactttatt
     1141 tacgcacgaa agaattacac gcgaagtggt caggtggtcg aattcaaaac tgggctatgg
     1201 tgcttaatca gttaatgatc aacgagacct tctcctctag aatgaaaagt atgcgattta
     1261 ccttccgtaa caacggtagg tttattcaac tatgtgtccg atcaaaattt atctgatttc
     1321 aaaatagccg agagaattat ggtggttaaa aactcttaac tatttaactt ccacagttta
     1381 gttgacaaac ccacttaatt ctgataacga aaaacatatt cgaatgatta aacagaagta
     1441 aatacttatt taaataactt ttaattttca tgttcataaa tgatttgaaa ccaaagcaaa
     1501 tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa gtagtcattt
     1561 gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag ataccagttg
     1621 aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg aaaattattt
     1681 ttcatttcct tttattccta ttaaaatcag tgatgtcaat catattaaca agactaaaaa
     1741 tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac tactatactt
     1801 acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc aatcaaaatc
     1861 actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta tggtcgctca
     1921 tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc gcacgacggt
     1981 gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacagc atggaaaagg
     2041 aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat atagttttac
     2101 cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct tcaagaagat
     2161 tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc ctttatttca
     2221 attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat cctatttgcc
     2281 agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt tatataattt
     2341 gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt atgccagcat
     2401 tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag tgctccattt
     2461 agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa gtgtagcgcg
     2521 ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa ttggaggaaa
     2581 gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa atactacccg
     2641 agaaattcga caacaaccgg aactgtggca tgagacgttt gaccgttatc aagaaacagt
     2701 tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc gaccaatccg
     2761 agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa ttccgtttct
     2821 tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga ccgatatcgt
     2881 tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct catttgcccg
     2941 tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc tggtcaacaa
     3001 gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac aaatgcctac
     3061 aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg gttttgctat
     3121 gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc aagcaagcaa
     3181 tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag tcatccaacg
     3241 agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt acttaggttc
     3301 gggaagttta ggcggattga ctagagaagc gcaattaaaa gttctcgagt taactgctgg
     3361 aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca aatcttttgt
     3421 caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc agaaatacga
     3481 tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg ttggaattgg
     3541 tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt cgttactgcc
     3601 ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat tactttgttc
     3661 ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca atcgtgtggt
     3721 caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca tgttagaatg
     3781 gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca ttattaccgc
     3841 tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag ggatggaagc
     3901 gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga ctccttatgc
     3961 ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc gtgcagcagc
     4021 gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac ccggccgctt
     4081 gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg cggatgcggt
     4141 gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc aaaagcaagt
     4201 ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt atttagagtt
     4261 attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca aagcaaaacc
     4321 gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag tagatgtctt
     4381 aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag gggagatcgc
     4441 ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa cagatttgcc
     4501 gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat tgcgttttgc
     4561 caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt ggaaagatgc
     4621 agtcgctcct tttgttgaaa aaggagaagc ggcaacttta gaatggctga cagaacaagg
     4681 gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc catggtatga
     4741 gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata agagatatgt
     4801 gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata aaagattgtt
     4861 agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc aggcaatttt
     4921 ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc cttacatggg
     4981 aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa ttttgtttaa
     5041 tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa aagagaatcc
     5101 aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag aacctgtaga
     5161 caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa tcgtttctcc
     5221 tgagacttta aaaaaggcaa atgaattagg ctttaatttt atttgtttaa ctggaaatcc
     5281 aggaacagga gtatctaata gaactattga gaaagcaata acattatcca aatcaatttt
     5341 taatggatta attatcgctg gaaaaatgca tggtgctggt gttgatgaac caattatgaa
     5401 cttagatata gcaaaaagat ttattaaaac aggtgtagat attttattag ttcctgctcc
     5461 atacactgtt ccgtatttca tggaaagtga tttgcgagga atcgttgatt atataaaaga
     5521 ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta atggaacaag
     5581 ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa aagcatgtgg
     5641 agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag aaaatatttt
     5701 tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag gaaaatctaa
     5761 ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa tagctgcaaa
     5821 aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag ttatgccatt
     5881 aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag gatttacaaa
     5941 atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt caacatttgg
     6001 tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga cttatggtgt
     6061 agactcatta tcagcaggat tattatcttt gagtggatat gttttactta cacctttatt
     6121 ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg gtctttttgt
     6181 tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac ttaaaaatat
     6241 tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg ttgctattat
     6301 tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt ataaacttgg
     6361 aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat ctttactgac
     6421 gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt ggatgtttgg
     6481 tattcatggt gcacaggtaa cggggccaat aattgaaccg ttattattgc aaaactctga
     6541 tgcaaataga attgcttatc aagctggaaa agaacttcct aacattataa cttatgaatt
     6601 cttatataac tttgtttttt ctggtggtgc aggttgttta tttgcacttg ccattttaac
     6661 atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa tcgctccagt
     6721 aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa atataaaaat
     6781 gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt tttcaatgag
     6841 tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc caccgataat
     6901 tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag taataacaat
     6961 tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg ccaagctaaa
     7021 aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagttaag tatgaaaaga
     7081 ctatttttaa atttagcatt tttgcgctat cggcgtagat gaagtatcac gataacgaaa
     7141 ttagtttctt tcatttag
//
LOCUS       DACERL010000106         6643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.111, whole genome shotgun sequence.
ACCESSION   DACERL010000106 DACERL010000000
VERSION     DACERL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.111"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1..859)
                     /locus_tag="IXF54_002592"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IXF54_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IXF54_002593"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IXF54_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1405810.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IXF54_002594"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IXF54_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IXF54_002595"
     CDS             complement(2143..2373)
                     /locus_tag="IXF54_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1405811.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IXF54_002596"
     CDS             complement(2603..3421)
                     /locus_tag="IXF54_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1405812.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IXF54_002597"
     CDS             3582..4271
                     /locus_tag="IXF54_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1405813.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IXF54_002598"
     CDS             4285..5787
                     /locus_tag="IXF54_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1405814.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IXF54_002599"
     CDS             5800..6276
                     /locus_tag="IXF54_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1405815.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IXF54_002600"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IXF54_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1033 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttccttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACERL010000107         6462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.112, whole genome shotgun sequence.
ACCESSION   DACERL010000107 DACERL010000000
VERSION     DACERL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.112"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(105..560)
                     /locus_tag="IXF54_002601"
     CDS             complement(105..560)
                     /locus_tag="IXF54_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405816.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IXF54_002602"
     CDS             complement(714..1922)
                     /locus_tag="IXF54_002602"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1405817.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IXF54_002603"
     CDS             complement(2034..2366)
                     /locus_tag="IXF54_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1405818.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IXF54_002604"
     CDS             2514..3389
                     /locus_tag="IXF54_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1405819.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IXF54_002605"
     CDS             complement(3471..4052)
                     /locus_tag="IXF54_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1405820.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IXF54_002606"
     CDS             complement(4058..5317)
                     /locus_tag="IXF54_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1405821.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IXF54_002607"
     CDS             complement(5319..6365)
                     /locus_tag="IXF54_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405822.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1800 a         1337 c         1102 g         2223 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACERL010000108         6287 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.113, whole genome shotgun sequence.
ACCESSION   DACERL010000108 DACERL010000000
VERSION     DACERL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.113"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(23..958)
                     /locus_tag="IXF54_002608"
     CDS             complement(23..958)
                     /locus_tag="IXF54_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1405823.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1260..3194
                     /locus_tag="IXF54_002609"
     CDS             1260..3194
                     /locus_tag="IXF54_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1405824.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            3651..4067
                     /locus_tag="IXF54_002610"
     CDS             3651..4067
                     /locus_tag="IXF54_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1405825.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            4081..4647
                     /locus_tag="IXF54_002611"
     CDS             4081..4647
                     /locus_tag="IXF54_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1405826.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            4670..5656
                     /locus_tag="IXF54_002612"
     CDS             4670..5656
                     /locus_tag="IXF54_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1405827.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(5811..>6287)
                     /gene="scm"
                     /locus_tag="IXF54_002613"
     CDS             complement(5811..>6287)
                     /gene="scm"
                     /locus_tag="IXF54_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1405828.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1836 a         1162 c         1335 g         1954 t
ORIGIN      
        1 ttttattagt atgattatgt gattattttt ctgttacgat attatctact tcgattgttt
       61 gagtgtcgcc tttttcagct gcattcacta attgttgtgc gtacatgatg aatgaatgtg
      121 agctgtgtgt agcacctgta acaacttcta cattagcagg tgagccatct tcttcgccca
      181 ttgctttgac gaattcgtcg ttcagagtag ggatgtaagt ttttggttct gtaccgcttt
      241 ttgctttcat attttcatta tattctgtat catcttgttt tgatttaccg tctttgtcga
      301 tataatcaaa gtttgattca gttactttgc cgccagcaac tgtcatttcg aattgcacac
      361 ggtagccgtt agagtagttc ttttcttcaa gcttgtaagt gccgtctttc aaatcagcac
      421 cattgtcgat ttcgattgta tctgtgttac cagcttgagc agcttggatc aattgttgtg
      481 cgtagttttg gaagctttca gatgagtgag tagctccagt tacgacttcc acaccgcttg
      541 cgctttgtgc ttttaagaat tggtcattca actcaggaat gtatttttct gggtttgtgc
      601 cagattttgc ttccatgttt ttgttgtatt ctgcatcttt tgtttttgat tcgccgtttt
      661 cattgacgtt atcatatttt gattcagtga ttttgccgtc ttttacaacc atttcaaaga
      721 ctgcacggta accatttgaa taattttttt cttctaattt atatgtgccg tcttgcaaat
      781 ctccgccagc tacaactttt gcagatgatt ctgttgtgct ttttgcagtc gaagattgtg
      841 ctgtagatga ttcagaagaa gagtttgagc ttccgttgtc atctgcgccg caagcgccta
      901 acactagagc tgaaaatgct aatacggcaa atccagttac aaaacgtttt ttcttcattt
      961 taatacccca ccattccatt tctagtccac aatttttctt tgtgattcat tatacattct
     1021 aatcttttta gcctataagt caactatttt tgataaataa cccatgtaat atttcaaacg
     1081 ttttctttca gcaaatttcg tgaaaaaacg ctttatttta aaatttgaaa aaaatccaaa
     1141 aggagttaga caaaagaaca gaacatctat ataatatatg gagattgtgt aataagtatc
     1201 aaactaaaag aattatcata tattcattat atagatagtc tataaggagc ggatgatcaa
     1261 tggcaaagca aaatattgtc gttgtaggag caggatatgc cggtgtttca gctacaaaat
     1321 acttggcaaa gaaactgaaa aaagaagatg tagcaattac tctgatcgat cgtcattcgt
     1381 accatactat gatgaccgag ttgcatgaag tagctggcgg acgtgtagaa ccagaagcaa
     1441 tccaatacga tctgcagcat ttgtttgctc gtcaaaagaa tgtacaactt gtcacagaca
     1501 ctgtaatcgg tatcgataaa gaacgaaaaa tcgtcaagac caaattaggt tcctatcctt
     1561 ttgatcagct gatccttgga atgggcggag agccgaatga ctttggtaca cctggtgtca
     1621 aagcacacgg attcaccctt tggtcatttg aagattctgt aaggattcgg gaacatattg
     1681 agaagacagt agcaaaagct gcattggaac cagatgatga actgcgtaaa gccatgctga
     1741 ctttcgttgt ctgtggttct ggatttaccg gtattgaaat gattggggaa ctgatcgact
     1801 ggaaaaatcg cctagccaaa gattataaat tagatccaaa cgaaatcaca ctgatggtcg
     1861 tagaggcaat gccgacgatc ttgaatatgt tggatcgaaa tgatgctgca aaagctgaac
     1921 gctatttgcg taagcacaac gtggaattat tattgaatgc accaatcgta gaagtggctg
     1981 ctgatcatat caaattgaaa gacggacgca acattccgac tcacacactg atttggacag
     2041 ctggtgtcaa aggaacttct gacgctgctg actttggatt agaatcagca cgcggacagc
     2101 ggttgatcgc caatgaatat atgcaggcaa aaggctacga agataaaaac atctatatca
     2161 ttggtgattt agtcttttat gaagaattcc caggaacacc gacaccgcag atcgttcaag
     2221 ctgctgaaca aacaggacac acagccgcag cgaatatcgt ggcttcgatc aaaggcggag
     2281 aaaagcataa atttaaagga aactatcaag gatttatggt ttctattggt gccaaatggg
     2341 gggtagctaa cctatttgat aagatccatt taagcggatt tttagctatc gtaatgaaac
     2401 atatcgtgaa tttgaaatac ttctttgata ttcgttctgg ttattatatg ttccaatatt
     2461 taatgcatga gtttttccat atcaaagatg atcgcaacgt gactcgagga cattcttctc
     2521 gttacggcaa tgtcttatgg agtgtgccac ttcgtatttt ctatggttta gtttggttcg
     2581 ttgaagcttc caaaaaaatc attgggaatg gtgaagtgtt caaaccaagt acttggtttg
     2641 gtgaaggatc atggttcact ggaaacagtg tgttcccttg gacatgggaa tacaatgtaa
     2701 cagatgttgc cagtggtgct tctgctgctg gtgatgcaac cacagctgcc agcggagcag
     2761 gggcaagtgc cggagaagca gcgactcaag ctgcacattt tggtttgagt tatgcatacg
     2821 gtgaagaacc aatggctgtg attgagaagt caccagactg gttcacaaag atcatgcaag
     2881 tgatcatgcc aaacaatgat gtagcaatgt tcttccaaaa attcatggta ttctttgaaa
     2941 ttgccttggc attagcattg attgctggat tattcacatg gctatgcagt gcgacaacga
     3001 ttgtattggt gatcacgttc tgcttatcag gcatgtttta ctgggtaaac atttggttca
     3061 tttttgttgc tttcgcattg atgaacggtt ccggacgtgc gattgggttg gatcgctggg
     3121 taattccatg gctacaacgt acactaggta attggtggta cggtagacca agttcccagt
     3181 acggcaagaa ataaatagat aaagaacgtt atttgaatgc aagatttcaa tagaaagggg
     3241 ctgagacaaa ccgtcacagc ctccttttta tgtaaaatta aaggaataag atgcccgaga
     3301 taagcctcca tgagcaagac acaggtgtca caacctctgt tacaacctct tgttataaac
     3361 ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa cttgaaaagt
     3421 aattttcgta aattccttct tatttcttga agttgcccac ttctgtcaca acctcttgtt
     3481 ataaacggtg ttccacctgc tgcttctcgg taataagccc aaagaattag aaaacatgga
     3541 aagctgtttt ctattcttcg ttcttattac tcaaagcaag acacaggtgt cacaacctct
     3601 tgtttttatg ctaaaattgg gtgagttaat ctggaaaaag gagaacaaaa atgagcctta
     3661 aagaatttgc tgaaaaaagt ctgctcaaac cttgggatgt ttttattatt ttgcttttga
     3721 ctgttctttc ttttttacca gtcatgattt ttggtcttca gcaaaacacc gcaacagcag
     3781 aaaaagaagc ggtactgcgt gtggatggca cagaaatcaa aacattccct ttgatggctg
     3841 gagaagagag caacacatat acttacaaag atgaacacgg agattataac ttgatcgaga
     3901 tagatggcga tcgagtacgg ataaaagaag cagattgcga tgaccagatc tgcgtacgtc
     3961 gaggctgggc aacgaaaaat ggcgaaacca tcgtctgttt gccacataaa ttagtgatcg
     4021 aagtccgttc aacagatgga ggtgacgaag acagtctgat ttattagacc ttcacacacc
     4081 atgagtaaat tacaaaaaat gatttatatt tcgctacttg ttgcacaagg agtgatcatt
     4141 ggtttattgg aaaatatgat cccttatcct ttttcttttg cgcctggtgc aaaactaggc
     4201 ttagccaatc taatcacgat cgttgccatt tttaccatga aaaaacgaga cagcttctta
     4261 ctactttggc tacgactatt cctgactact ttgctaggcg gaacgatttc cacattcctt
     4321 tatagtatga gtggtgcttt gcttagttat tttggaatgt tattggtcaa gcaattagga
     4381 ccaaaacgtg tcagtatcat cggaattagt gcggccggcg gtttcatgca taatgtcgga
     4441 cagcttttga tggcttcttt cattgcacaa tcatggacag tcatgttgta cttgccagtc
     4501 ctttcagttt tagggatttt atcagggatt gcaatcggga tcgcagcgaa ttatctttta
     4561 cgacatgtgc agacacttca acgattccaa aaagattatg agcagggaac caaacaaaaa
     4621 tggagcccaa tgtttaccaa aaaataatcg tcacacaagg agttttttta tgaagctaca
     4681 tccaatgtgg gaaaactatc ctgctttaag taaagaattg acagaaacac taaaacggat
     4741 ggaagaaact gtccgcctga aaaacaaacc tgtcgaacaa gcaatcaaag aaacgatcca
     4801 tgccggcgga aagctgttgc gtcctgccta tcagctattg ttctcacaat ttggaccaga
     4861 gcaagatcga gacaaagcca tcgctttggc agcttctatc gaaatgctcc atacggccac
     4921 actgatccat gatgatatcg ttgatgaagc ggatctgcga cgtggtcagc ctaatatccg
     4981 tagtcagttt ggtaatgtcg tttctgtcta tgcaggagac tatttatttg tctgctgttt
     5041 taaactattg tctgattact ccacttcctt gaaaagtctc caattgaatt ctcgcagtat
     5101 ggaaaaagta ttgaatggcg aattagggca gatggatgat cgctataatt atgaactttc
     5161 tgtaaaagaa tatttggaaa atatttcagg taagacagct gaactgttcc aattaagttg
     5221 ttctgttggc gcttttgaaa gtggaacaag cgagcgcttc gcaaaaaaag caggagatat
     5281 cggactttct atcgggatgg cctttcagat tatcgatgat attttagatt atacaaaaga
     5341 gagccaggaa atcggcaaac ctgtactaga agacatgcgt caaggggtct attctttacc
     5401 gttgatctac tctttgcaaa aaaacaaacc aaagctacta ccttatttag agaagaaagc
     5461 ggcaatgaca gaagaagatg tcgacgccgt acgcaaaatc gttgaacata caggaggcgt
     5521 ggaagaagct aggaagttag cggcctctta cacagaaaag gctttgaagg aaataaagaa
     5581 attacctgcc acctctcttc gaacaaaaga gaatctcttt tcccttaccc aactgatttt
     5641 ggatcgcaaa gactgaatag ggaaaggttc gatacgtaaa taaatttctc agaaggaaat
     5701 ctacgtgttt tttgacaaat tgaattgtag ttcttgtatt tatgataaaa agcaaaaata
     5761 aagagcaacg tccgttagcg acgttgctct tttttatctt gaatattttt ttaccttttc
     5821 gatttcttat aactcataaa agtgattctt aaaccgatta caagtaatat aacgcctgcg
     5881 agtgttatcc aattattcga ttgacttcca gttttaggaa gtgctctttt tgatttgttt
     5941 ccattgtcat tgcttgtttt attagttaag ttaaagttcg tcctgttatc cgaacttgtt
     6001 tttgagaagg agacacttgc atgacgtgta tcgcttgtgg tagacgacgt gtctgtacta
     6061 tcgcttgaag ttgatgaatc tgagctatca cttgtggtag aggatgtatc tgtactgtca
     6121 cttgaagctg acgtgtccgt gctatcactt gtagtggtag atgaatctga gctatcactt
     6181 gtggtagagg tagtatctgt actgtcactt gaagctgacg tgtccgtgct atcacttgtt
     6241 gtggtagatg tatccgtact gtcgcttgaa gctgacgtgt ccgtgct
//
LOCUS       DACERL010000109         6069 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.114, whole genome shotgun sequence.
ACCESSION   DACERL010000109 DACERL010000000
VERSION     DACERL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.114"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..134
                     /locus_tag="IXF54_002614"
     CDS             <1..134
                     /locus_tag="IXF54_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1405829.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="IXF54_002615"
     CDS             complement(220..1314)
                     /locus_tag="IXF54_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1405830.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="IXF54_002616"
     CDS             1503..2264
                     /locus_tag="IXF54_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1405831.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IXF54_002617"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IXF54_002617"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1405832.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="IXF54_002618"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="IXF54_002618"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1405833.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="IXF54_002619"
     CDS             complement(3904..4917)
                     /locus_tag="IXF54_002619"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1405834.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5082..6002
                     /locus_tag="IXF54_002620"
     CDS             5082..6002
                     /locus_tag="IXF54_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1405835.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1863 a         1205 c         1081 g         1920 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gtcattttat
     4981 tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg
     5041 gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa
     5101 ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa
     5161 atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc
     5221 aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa
     5281 aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc
     5341 gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt
     5401 ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg
     5461 gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg
     5521 gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact
     5581 taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg
     5641 atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg
     5701 caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct
     5761 atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa
     5821 ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg
     5881 tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg
     5941 tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat
     6001 aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaagaac
     6061 acttttgtc
//
LOCUS       DACERL010000110         6026 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.115, whole genome shotgun sequence.
ACCESSION   DACERL010000110 DACERL010000000
VERSION     DACERL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.115"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(25..1497)
                     /locus_tag="IXF54_002621"
     CDS             complement(25..1497)
                     /locus_tag="IXF54_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1405836.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFIY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVMEGKVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQEKIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(1511..2359)
                     /locus_tag="IXF54_002622"
     CDS             complement(1511..2359)
                     /locus_tag="IXF54_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1405837.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LTSFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2356..3222)
                     /locus_tag="IXF54_002623"
     CDS             complement(2356..3222)
                     /locus_tag="IXF54_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1405838.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(3265..4578)
                     /locus_tag="IXF54_002624"
     CDS             complement(3265..4578)
                     /locus_tag="IXF54_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1405839.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMESGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDGSA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQVIDTSLK"
     gene            complement(4720..5691)
                     /locus_tag="IXF54_002625"
     CDS             complement(4720..5691)
                     /locus_tag="IXF54_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1405840.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGFSPYPNETNNRKAGFYNICEKNKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            complement(5817..6026)
                     /locus_tag="IXF54_002626"
                     /pseudo
     CDS             complement(5817..6026)
                     /locus_tag="IXF54_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1821 a         1304 c         1021 g         1880 t
ORIGIN      
        1 taaatcatca ctgagctctc ccttctatag attaattaag ccgctttcca atgaaaaggt
       61 tacttcactg tctgccagca aaagaaaatc ctgtcctttt tctgtctcat aaaaagttgt
      121 actcatagtc agacgatcat ttagaaaaag ctcaatagct gaagtatcac ggaaaatttc
      181 gatggttaga caattatttt ccaaaggaat cgtcacactg cgctttttca acacttcctt
      241 ttcagctcct gataaatcat acggcatgtg ttccctggaa gcagtaaagc actcccgttc
      301 aacatcatag ttaagcagta atgtatggtg gggtcccttc gcatagtgaa gttctagttt
      361 cgttgcctgt tttaaattga tttccaagcg taaatagtgc tggtcatata ctaagtcctt
      421 cagtaagaat cgattctctt cagtcttcgc ccattcaatc ttttcctgcg gatcaattac
      481 ttgataaatt gatgaaactg gccgttgcaa caattgatta tcagccaccc tcagctctct
      541 aggcaaggtc atggaacctg accaaccatg gcccagatca ttcgtaggta ttttacgctg
      601 ccacatttgc atccaagcag tcattattct ttggcctgct tcgttttcac aggtctgggg
      661 ggcataaaaa tctaaaccgc catcgatttc atgaaaattc tctactagaa atttgcctgt
      721 ttgccaatcg acagtaccaa tgaaagcaac agttgagttc gtattttggt aagcattgcc
      781 ttgtggagtc atttgaatag gagaaagaat caagacttct ttgccatcta gttcaaacaa
      841 gtctgggcat tcccatacaa tgccttgttc tggagttccc tccagcaata cggaataaaa
      901 ctcccaagtt agtaaatctt tggaatggaa catcacaatt tgtccacgag catctgcagt
      961 tttagtagcg actacagtaa aatagtcttc ttttcgctga aacactttgg gatcgcggaa
     1021 atcttcggtc gttgcaacag aagctaagtg atcttctgcg atgattggat ttccggcata
     1081 tttttcaaag tgaatgccat cttctgaaac tgccaaacac tgaacttgtc gaactttccc
     1141 ttccataaca ttgtgtcctg tataaatcaa atacagcttg ccatcctttt ctatagcact
     1201 gccggaaaaa cagccttcct tgtcatagct ttcacctggt gccagtgcta ctggcaaatg
     1261 ctcccagtga attagatcct tgcttttagc atggccccaa tgcattggtc cccagatgct
     1321 gtcgtaagga taatattgat aaaacaagtg atactcctct tgataataaa taaaaccatt
     1381 tggatcattg atccagccaa tcggagccat caaatgaaat ttattccggt attcttgatt
     1441 gactgtttga gcatttttct caataaactc attggccttt aaaagttttt gcaacattca
     1501 ctattcactc ctattttata cccgttccag aaccgccaag tccctgcaga atatattttt
     1561 gtgcaacgat ataaacaata atcagtggaa tcgttgcaat ggttaaaaca gccatcactt
     1621 gattgatata aactggctgt gtagtattga tcgtagtaat tgccacttgc attgaaaatt
     1681 tgctgctgtc agttaagacc attaacggcc aaatataatc attccagctg gagataaagg
     1741 ataagacacc tactgtggct atggctggtt tagacattgg cagcatgatg tgaaagaaaa
     1801 ttttaacaat atttgcacca tctaatttcg ctgattcaat gatttcttct ggaatcgcga
     1861 taaagaaatt tctaaacaaa tagatattaa acgcgctggc catcgctggt aaaatcactg
     1921 ccaagcgagt attcactaag cctagtgaat taacgatggt aaattgcgga attaaaaccg
     1981 tctccatcgg gacaatcagc agagctaaca aagctccaaa gataattttc ttgcctgaaa
     2041 aattaacctt cgcaaaggca aaacccgcca aagagtttac aatgattgac ccacaggcaa
     2101 aggttaaacc ataaaaaata ctgtttaata aataagttcc cacactgaac cgggaaataa
     2161 cttcttgata agttttaaac caattagctg gattcaaact aggtaaaaaa gctttgaagg
     2221 aagttaaatt ttgatagaca tctgcttcag gcttcataga ggaaactacc atccacagca
     2281 ttgggaaaat gaataatgcc gctaaaagga acagcaaaca atattctacg atcattagcg
     2341 gtccaaattt cttagtcatg ctggatcatc ctcctttacc aattttcttt gagcaattgt
     2401 aattaaacca atcatggtgg taaagattaa cgcaatggag ctggagtagc ctaccatacg
     2461 gtctgtaaat ccagtttggt aaatgtaata aaccattgtc atagtagaat tcattggtcc
     2521 accttgagtc attaccattg gctgaatgat tagtttaaag gccgaaatca atgtggtaag
     2581 taaaataaaa atcgccgtgg gctttaataa tggcatggtg atatagcgaa attgctgaaa
     2641 cttagaaaaa ccatcaattt cggctgcctc ataaacgtct tgcggaatgt tttgcattcc
     2701 ccctaaaaac agcagcatct gatatcctgc accttgccat gccgatacga aaacaagcgt
     2761 atacattgct tgtttgggac tagtcaaaaa aggctgcgaa gcaattccaa tctttgcaaa
     2821 tagcgtatta accagacctt cattaggatt taacaagtac aaccataaaa tcgaaataac
     2881 gactaaggac atcacgactg gcgcaaaaaa ggctacttta aaaaagatat ttcctttacg
     2941 atttttgttg ataataagtg ccattcctaa agctgcccct aattggaccg gaataatcca
     3001 aacaacaaat ttagccgtgt tccaaatact ttttacaaaa attggatcag cgaacagtcg
     3061 cttaaaattt tctaagccga taaattgtct agcatctggc gttaacaaat aataatcagt
     3121 aaaggcataa taaattacca tgattacggg tatgatcaga aaaatcgtca gcagcagcaa
     3181 agctggcgct agaaatccgt aacctatcaa attgtctttc attgtcggtt tttttaacac
     3241 ttgattggcc ccctttagaa acttctattt tagtgaagta tcaattacct gttgcatttc
     3301 agcagcgcga ctatctgcta ctttttgtac atctgggttt tcctcgaagt agctaatatc
     3361 ttgaactgct tgttggaatg ctctagatac ttgcggataa gctacaacca ctgggcgggc
     3421 atgtccagaa atttggtttt gcttaattaa tacctgcatt ccttccgaca cttcatcttt
     3481 aattaaatca actgttgaat ttctgatagg tagaacactg ttccctaaac tcataatacg
     3541 actagattct gtactggttg caaacttaat aaaggccgca gaaccatcct ttttctctgt
     3601 tttctcagtc attgctaact gccagctgcc ggttggtgaa actaactccc cagttgcatc
     3661 tgaaacgggg taaggcaaaa taccataatc aatctcttga tagttttcct ctaaatctgc
     3721 cattgtccaa gaaccgctca gcagcatggc atatttgcca gtttcaaatc ctttttcaat
     3781 aggactgatt gtggtgtaac ctttttcaac caattcttga ataaattcca acgcttcaac
     3841 ggatttttta tcgttaaaat agtcttcggc tttggttcca tcagcattaa cgacatcgcc
     3901 gccattcgac catacaaatg gtgtatatgc gtaggtcagc atttcgtctc ccgactccat
     3961 ttgcatatca attgcaggcg ttttgtattt atcagtcagc ttttggcaaa ttgcttgaaa
     4021 ttccgtccaa gtccaaggat cttctaacgt cggcaattga tcttctgtaa ttccagcatc
     4081 tttgaacaac gacttattgt aatagacacc cacactggac tctgagaaac caaacgcata
     4141 cattttgcca tcgtaggttc cttgctgaat aatgctgtct aatacatcat ccatgtccgc
     4201 atctttcaaa taatcatcta atggagcaat gactcctgaa tgagcataag ctgcagtatt
     4261 cggtccatct aacgtaataa catcaggaag ggtattggtt gtaattgcag catttacttt
     4321 atcctcatat ccaccgccgc ttccgctacg aggaatatac tcagtaacag ctttatattc
     4381 accctcgtat tcatcattaa aattgttcac aatctctgcc caagcttttc cttcaggcgt
     4441 ttcatcagaa aactgaaccc aaatcttaat ttctcctgaa tcactgctgc ctgaactttt
     4501 ctccttactg ccacatcctg caagtcctcc agctgttaat gctaaaattg ctgcaattgc
     4561 taacattctc cttttcatcg atgttcctcc tattttttga aacgtttccg ttttattctc
     4621 caattaattg gtaacgtttc caattaattg gttttaagat atcattgaaa ccactttctg
     4681 tcaacctatc tcaaaaaaaa atcgttataa accggttttt tactctttag tcgtttctcc
     4741 agcgtgaaag aaaaccggta ctaccacacg cttaggcggt tcttcattta aaatcatcgc
     4801 catgagtgtt tctactgaaa ccttggccat ttcctctatc ggctgtacaa tcgtcgatag
     4861 taaatagtct ttttgcaacg atagcttgac tccatcaaaa ccaataattt gaaaatcctt
     4921 aggtgcttcc ttccctagtt ttttttgggc ttttatcaca tccaaagcca tgaaatcatt
     4981 gatggtaaaa atgctatcaa tttcaggatt atccgaaaaa aaagcgagca attgttcctc
     5041 caaattgcta attggttcag gcatatccaa taccttcaca gctattttat ttttctcaca
     5101 aatattgtaa aaaccagctt tacgattgtt tgtttcgtta gggtaaggtg aaaaaccccc
     5161 aacatagcac aagtttttac agttacgagt aagtagttct tgagcggcaa tttgcccacc
     5221 taaataatta tctgccgtta cataagaaac ctgttccgaa aaatggcggt ctatactgac
     5281 aaatggtaaa tttgatgata catatttatc aatgtctgaa taggtaatac ctataattcc
     5341 gtctacttta ctctgcttga ccatttggat atattctttt tctttttccg cactgccgtt
     5401 cgcattacac aaaaaaagct tataattttt ttggctgaga gcttcctcta catagtaggc
     5461 aaactcggaa aagaagggat gccaaatcgt aggtaaaata agagctattg tattgctgct
     5521 attcgttttc atacctctgg catattcatc aggctcataa ttaagctcac taattgcagc
     5581 cattactttt tctttggtaa cctctttcac ctttactcca tttattactc tagataccgt
     5641 acccacacca actcctgcta acgcagcaac atccttcata gtagttttca cttcacgaac
     5701 aactcctctc aaaagaattt caggtattat tataccaaaa gaagaaaacg attccaaatt
     5761 agcaggaata tttttatttt aaaatagata ttagacgtga agcttttcgt aaaaaaattt
     5821 tcgtattcag atttaccgga ttgactctgt cgccaatata ggccggcata ctttgtcaat
     5881 tttatttcat cagaaaaacg ttcaattgga ccaatttcag cgagtattcc agcagcctaa
     5941 acaggaccaa tacctgggat actggttaaa cattgatact ccggtagcac cacgactaag
     6001 tcgtcaatgg ctttatctaa gtcttt
//
LOCUS       DACERL010000111         5989 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.116, whole genome shotgun sequence.
ACCESSION   DACERL010000111 DACERL010000000
VERSION     DACERL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.116"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..279)
                     /locus_tag="IXF54_002627"
     CDS             complement(<1..279)
                     /locus_tag="IXF54_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405841.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVL"
     gene            complement(286..564)
                     /locus_tag="IXF54_002628"
     CDS             complement(286..564)
                     /locus_tag="IXF54_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405842.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(524..1438)
                     /locus_tag="IXF54_002629"
     CDS             complement(524..1438)
                     /locus_tag="IXF54_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1405843.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1420..1800)
                     /locus_tag="IXF54_002630"
     CDS             complement(1420..1800)
                     /locus_tag="IXF54_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1405844.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(2250..2537)
                     /locus_tag="IXF54_002631"
     CDS             complement(2250..2537)
                     /locus_tag="IXF54_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1405845.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2557..2781)
                     /locus_tag="IXF54_002632"
     CDS             complement(2557..2781)
                     /locus_tag="IXF54_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ1405846.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(3421..3954)
                     /locus_tag="IXF54_002633"
     CDS             complement(3421..3954)
                     /locus_tag="IXF54_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1405847.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(3975..4919)
                     /locus_tag="IXF54_002634"
     CDS             complement(3975..4919)
                     /locus_tag="IXF54_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1405848.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            5556..5642
                     /locus_tag="IXF54_002635"
     CDS             5556..5642
                     /locus_tag="IXF54_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1405849.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(5763..>5989)
                     /locus_tag="IXF54_002636"
     CDS             complement(5763..>5989)
                     /locus_tag="IXF54_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405850.1"
                     /translation="KDTQTEEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQ
                     ATNIQNPTKKETALNYLKSVKEWLDQQQQN"
BASE COUNT         1593 a         1118 c         1013 g         2265 t
ORIGIN      
        1 taacacttgc tcctttttct ttttatcaca tgctgcttca tacttttgag tgatatcccc
       61 taataaaata cttcttgaaa tacgtttatt tccctctaaa acgccgaata aaccacttaa
      121 aacgattgat aatgtaaata taacccctgg caataaaaac ctcttaaatc gaattttaga
      181 gccttttaat gaatatcttg tttgataagc cttatagcta agccaaataa ttcctaaaat
      241 actggcaaaa atgagtagat agggtaaata gaatgccatt tctatttatt ctccccatac
      301 cagccaaaag gctaacagtg tcatgattaa gcaacataag ggggcaagaa tatccattga
      361 tcgctgcata attacttttt tctgttcttt atttcgctgg tttaatagcg catggtggaa
      421 ttttttgact tgtgaatcaa ttgtagtttg tattctttct ttggtataag gtgcaagaaa
      481 agcacgttgt ttctttattt cattttctat gtcttgtaac gttttagaac gataattctt
      541 gttggtagtc tgctcctctt tcaacgtgtt ggtcatggtt tcttcgagtc ggtctaattt
      601 ccggttgttc agtcgtaaga gttcgttgtt ctcttcgtac ttcttttgca gtttcgcgaa
      661 tgctagctcg agctcttcgt agctcgttgg ttcgttgttg attgtaggct cgttctcgtc
      721 tgtccagctc atgtaaaatc gtctcctttt cgtaatcctt tcctagtttg ttggatcgta
      781 cttttttctt tttgtggatc aattcgtaag taaaattttt cccacgattc aagcaatgaa
      841 tgccgaatgt tttaagagaa tcacaaaatt ggttatacgt tcgagttttt ttgtttttca
      901 ttgtttgatc aatcgcaaac cgaatttcgt ctttccaaac aggttcattt ttttctttta
      961 atttgatttc agacatggtt cgtttttcgg gtttcttccc tcgttcagtg attgttaagt
     1021 catgttcaag acaaatctcg tcatttaggt cataacattt gtttaaaaat gcttgaccat
     1081 gggcttgaaa tttgttccca gtttccagat tgacggagtt aatgacaatg tggttatgaa
     1141 tatggtcttt gtctgtatgt gtgtaaatcg cgatttggtg gtcaggggct atttttttcg
     1201 catactctgc ccctagttga ttggcttttt cagcggtaat ttctcctggt cgaaaagact
     1261 ggatcaataa gtgtgcttgt acacgatcgt cttttccata cagcatgcga atttgtttca
     1321 tttcgctttt ggcataatca atgtcacagt ttatccctgt tttgacggtt gctcgtggtt
     1381 cggcgtaatt gatcgctcgg ctacaagaag tggttcggct tactttaact gttgccatat
     1441 cttttgtagc tccttttgca tgatttttag gttttcggat aagcgttggg cgtcgccagg
     1501 atcaacgtcg tgttggtgtg tgttgcacca cttggcaatt tgattgatat tcccgccagc
     1561 tttggctaaa tcgcgcgcga ttgcctgagc aatgtctggc gccactttcg gtgtcaccag
     1621 acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca ctcatttgca aagcttcagc
     1681 tgactgcttc agctttgcaa attcggattc gctcactcga aaactgattt gttttggctc
     1741 ggatcgatag gtatattttt tagaatgatt gctagccaaa tattgatttt gttcgctcat
     1801 aagtcccacc ctttcagtac gtcctacgga cgtatcgttc gcaataccat tgtacctaaa
     1861 aaggtacaat aagtaaagct cactaaagct cactaaaggg cttggcaagc tctatatttg
     1921 ctagccaaat acctacgtat ttggtgcaaa taaccgcttg tcaaaggggc gcaagctccc
     1981 ccttgaatcc ccctcccccc cactctccac aatacaaaaa agactagcaa atgctagcca
     2041 ctttttgtaa agtttcgctt gtttcacctt cggtgaaatt gttgcaaatc aataattagg
     2101 tagagctaca tttttttcgt tgttcaaaga ttcttatgcc atttactatt aataaatcta
     2161 taaatatgat aatttttcgg ctctataatt tatgggactg atgaatcaga attgattctt
     2221 cagtcccatt ttcattttat tccaaagaat taactccaaa taccaataga agcccatcct
     2281 ctatatactt gagattcctt actaacaatt tcttgtaatt ctattaaaga tttaggtaat
     2341 ccaaatttat gttttaaaat atatcttcca agaaaactac ttaatttcat actaacttca
     2401 ccaacacttt ttccctcact gatttcttta gaataaagaa agagcttttc ttttaagtca
     2461 ggatagtcat ctatagttgc ttcattgtat actttactaa ttgcatttaa aagtttttct
     2521 tctttagtaa aatccattat aataacctcc ctaccactac cttctaggtg cccatggacc
     2581 atgattaacc caaccattaa caataatttt tccaatttct cctttagctt gattccaatc
     2641 tacccaacat ttttctttgt tacaataaag accatttcca tagtaagtag ctgcatcaac
     2701 ttttattcct gtaccgatgc cagctagaca agttcctaat attcctagaa taacacaatg
     2761 ttttaatact ttctttttca tgattgtttt tccttttcgt ttttttaaaa ttgcttgtta
     2821 tattaaataa tcttagtaat tttattatat aacattttat tatataacaa tgattaacgt
     2881 cataaaaagt agttttgaat tcataatcgt ttaaataaaa ttataagatc agttatttaa
     2941 acagctagta gacataatgt gtatactacc aagtaacgcc tgtaccaagg gatccaggcg
     3001 ctgattttgt gaatttttat tggtctaggt taaaagctct ttttaaggct tggttttctg
     3061 cggttgtttc tttctaattc tttgtgaata ttgatcggtc attttttaaa tacttgttat
     3121 ttttgtttgt gtttgttatg ctttttacat atctcatatt catttattat tcttttctaa
     3181 ggttaataat aggataatct ctttcagaaa gactgctcgt acgagcagtc ttttcttatt
     3241 tatcaagttt ttctacaaat acttcctcat tttgactgtt gtagtaatta ggattgagtt
     3301 ttagttcccc ttttttattt cctttgttgg ccactgataa taacattggg ctaaagctta
     3361 atattcctag taggatagaa ccgaatagca agtgtttttt tgtcataagt atttccttct
     3421 tcatttccat agctgccacc attttttcca attctttgtt tgttcgtcca ttgtttctgt
     3481 tgcttcagaa agagtcgttg aactattctc tgaagactct tccgtataag tcaactgcag
     3541 ttgctcctga agtttttcga tttgttggtt ggcttgcagg gtgagttgtt gttgctggtc
     3601 gagtagtttt tgtatatgag aattattttg ctctttgagg gcaagttgtt ctcgtaaaaa
     3661 ttcattttct tcacgcaact gatctagcaa cttgtttgca accacgtttt tgtttggtat
     3721 ataagcattt ttatcgttgt tttgttcatg tttatgcaac ttatctagca acctttgatt
     3781 gtctaagttg cgagcaagtt gcaatactcc agaacgcaat acaaccttat aaccacgctc
     3841 atttttagct atcatggaag aatctaattt ttggatatgt ttcgatattg cttgcctact
     3901 catcttaaaa agatctgaca attcctttat tgtaaaggtt tcttgtggtt gcatactggt
     3961 tgcctcctag gttgctactg gaataaatta tctaaatcag ataataaact ttttttctct
     4021 ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt ttttctcttt ttctagttgt
     4081 ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt tgattttttc gttaattgtt
     4141 gtagcgggtt cttcgtattc tttttgttta tcagctaaag ccaatgcttt attttggtct
     4201 tctttggttg gtgcatgttt gttaaagtcg tctgcttttt tgatctcagg tttgaacgta
     4261 aattgatagc caataacagg ttttccccgt ccttttccgt atttcttttt gattgttagt
     4321 cctttgaaga ttgcagttag ttcttgacgt ataggtttta taactcgttt atctatatca
     4381 ggttgcttat aacttttagg aatatctaat aattcaagaa agtcttcttt ggagaaatat
     4441 gcgaaacctt tggttctgta ttgctttaac agacgaaaca tagtctttgc atagccactt
     4501 tgtagctcgt tgaattgttg taaagaatag cgtacccact cgtctaaatt atttaacaaa
     4561 ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat tttctacgtc cccgttgatt
     4621 ttaaattctg taaacattac aaacatatcg taagaaagcc cagactcact tctacgacca
     4681 aagcgtaaag ataagatttt ttcataagtt gcttttaaat cttctacaaa tcgtacattt
     4741 gcagttgctt tgtagttact taaatctttt agttggtcaa acgtaaaacg aactgtctta
     4801 tccccttttt cacgcattct agacacgatg gaaaaaaaca aattcatttc aaccggtgta
     4861 aattttctta aaggaattgt atttaattca tggtggtatt ttacaatctc attagccata
     4921 taatcacctc aatccaatta tgaatgacat tttttaaatg tcaagacatt catatttgac
     4981 gttcaaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa acagacaaaa
     5041 tgtccttcat aaacagacaa aatgtccttc ataacattct gtatccattg gtagagtaag
     5101 gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa aagagatata aaaagagggg
     5161 ggctaatttt cccgcctaaa aaccacttct tttttcttta aacaggttcg cgcatttttc
     5221 aaaactgcca atccaaaacc ttgcttccta cggtcgccaa aaaggtgtga tcgctacgct
     5281 ctcacattgt actgcgcagt cgcctaaagg ctcctttgct aaaaacatgt tggtaaagag
     5341 gcctttgagc ggttctgaat gcttatctaa gccgttttaa gacagtcttg gataaaaatc
     5401 cttatctagc tttaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccctaaaata
     5461 agcgatactt aaaaacacca cacaagttta attttttaag atataataac ttgtcgccta
     5521 tccgacagtc ttgcgactgg aaggaggtgt atctaatgtt tgaattgttc atttgccccc
     5581 ttcttataga agtttgtgtc tctttattca gttattggtt gaataaccga aacaagaagt
     5641 aaaatcaata aaggcgacat caacctgaaa aaaagcccca gagaatccga cctctctggg
     5701 gctttcgtgt atctaatgtt tgatcatttg catgtttagt ttaacatgat tgccgatcaa
     5761 attcaatttt gctgttgctg atctaaccat tccttgacac tttttagata atttaatgcc
     5821 gtttcttttt tcgttggatt ttgtatgttc gttgcttgct gaagcagtgc tttataacgt
     5881 tcgtaggtaa ctgattgttg ccgttctttt cgttcctgtt cactagttga aaaagggatt
     5941 gtatcactga ataaattata acaagcttct tctgtctgcg tatctttaa
//
LOCUS       DACERL010000112         5882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.117, whole genome shotgun sequence.
ACCESSION   DACERL010000112 DACERL010000000
VERSION     DACERL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.117"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1..191)
                     /locus_tag="IXF54_002637"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="IXF54_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="IXF54_002638"
     CDS             212..1414
                     /locus_tag="IXF54_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAQ1405851.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2147..3115
                     /locus_tag="IXF54_002639"
     CDS             2147..3115
                     /locus_tag="IXF54_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1405852.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3126..3941
                     /locus_tag="IXF54_002640"
     CDS             3126..3941
                     /locus_tag="IXF54_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ1405853.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4009..4719
                     /locus_tag="IXF54_002641"
     CDS             4009..4719
                     /locus_tag="IXF54_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1405854.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4755..>5882
                     /locus_tag="IXF54_002642"
     CDS             4755..>5882
                     /locus_tag="IXF54_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1405855.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2020 a          883 c         1187 g         1792 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt atagtacata
     1441 aaaaaattca tcttttttag tttaataatt caccaaaaag gtgagtaatg ccatttttga
     1501 aaattgagct tgttttatgt ctacatctgt atccgtatat aatccaaagg attataaact
     1561 cagaagagtc tttatttttt ctgaatataa ctactgtatc aaaaacaatc gattatgaga
     1621 caaaacaaaa tgacctcttt tatttaagaa aggaggtcat tttttgtatc ataaattgta
     1681 ttaaaatcaa tgtatcactt tttaatcaat tttaagacca taaattgcgt actaaaagaa
     1741 attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc acccatctac
     1801 ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg tgcaaaacga
     1861 tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca taattattta
     1921 aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag attatttata
     1981 ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat acaatacaat
     2041 tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca gttactttta
     2101 ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg atgtagcaaa
     2161 aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt atttggtggc
     2221 ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc
     2281 gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt
     2341 agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa
     2401 agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac
     2461 tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa
     2521 aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac
     2581 cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat
     2641 tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga
     2701 tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc
     2761 aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt
     2821 cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat
     2881 tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag
     2941 agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga
     3001 attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga
     3061 tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga
     3121 ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa
     3181 ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg
     3241 aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa
     3301 taaaaaaaat aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg
     3361 ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta
     3421 ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat
     3481 tgattcatat gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt
     3541 ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa
     3601 ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc
     3661 agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa
     3721 aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac
     3781 cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt tattttctcg
     3841 aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc
     3901 tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc
     3961 atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca
     4021 aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca
     4081 ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag
     4141 gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt
     4201 aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat
     4261 gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg
     4321 gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt
     4381 gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa
     4441 aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct
     4501 ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt
     4561 gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc
     4621 tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat
     4681 caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt gatttcaagg
     4741 agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat ggagagcagc
     4801 cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact
     4861 ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg
     4921 ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg
     4981 gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa
     5041 tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga
     5101 aaactgaccc atcagaagag ctattgaatg aaataaacta ctttggtcca atcacagcga
     5161 tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta
     5221 cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca
     5281 aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta
     5341 tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga
     5401 aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat
     5461 atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg
     5521 tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag
     5581 cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag
     5641 agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc
     5701 ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa
     5761 tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc
     5821 cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa
     5881 at
//
LOCUS       DACERL010000113         5826 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.118, whole genome shotgun sequence.
ACCESSION   DACERL010000113 DACERL010000000
VERSION     DACERL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.118"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..217
                     /locus_tag="IXF54_002643"
     CDS             <1..217
                     /locus_tag="IXF54_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1405856.1"
                     /translation="AQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGF
                     TKKTEKTPISELKHAKELREEWFTNDD"
     gene            207..473
                     /locus_tag="IXF54_002644"
     CDS             207..473
                     /locus_tag="IXF54_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405857.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            632..1633
                     /locus_tag="IXF54_002645"
     CDS             632..1633
                     /locus_tag="IXF54_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ1405858.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1975..2883)
                     /locus_tag="IXF54_002646"
     CDS             complement(1975..2883)
                     /locus_tag="IXF54_002646"
                     /inference="COORDINATES: protein motif:HMM:NF025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HAQ1405859.1"
                     /translation="MKQKQQFTKRQHFIPQFSIRPFEVAKGKCLIIDLKKKPLEVSIK
                     KTADIMQEIDLYESKDSEGNYINRNEIEDLYSKFENDISKKFKSLIELLLSNKSTSDF
                     LRMIKTNEWPSNEAALLSHIALTLIRSPYIKKLFYDNDNIPLFMKSIMYRQIIGSQMM
                     AVEHAKSHLNGEELEIALHVLKGNSDSGIQVILEHLMNNYQLEIYMSEGKGNFFLGDN
                     PILVNKFEDIDYLIPIAPSLCLGTTKLRLQNNRVLIKSNFNIVDDNMVRKINHLTLRN
                     ARQTVIVSTKKDLDIVKQFGSHLIVE"
     gene            complement(2908..3528)
                     /locus_tag="IXF54_002647"
     CDS             complement(2908..3528)
                     /locus_tag="IXF54_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1405860.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALDFVREQDVFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADTKDPQ
                     RRLVYRSIVQDLENGVAISTISKNYNITRQTVYRIKRDLSSDFPMN"
     gene            3979..5304
                     /locus_tag="IXF54_002648"
     CDS             3979..5304
                     /locus_tag="IXF54_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1405861.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRQKLAQMIQEHIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCFFLFDKYYEGQEVRHVGITYSKLIYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTAIVHASSMLEGARSITRSTLVGGHAGGNGGLKND"
     gene            5297..5647
                     /locus_tag="IXF54_002649"
     CDS             5297..5647
                     /locus_tag="IXF54_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405862.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANNNLAKEQMTTE
                     EISEVLNAAVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKYKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            5644..5823
                     /locus_tag="IXF54_002650"
     CDS             5644..5823
                     /locus_tag="IXF54_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405863.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
BASE COUNT         2019 a          857 c         1066 g         1884 t
ORIGIN      
        1 agctcaacgg ttgaaatggg tgaaaaaatt agacactaat ttgtacgaat taagaagtaa
       61 ggtaggatca aacatccaaa gagctatcta tttccatgtg gtcaatggaa ggtatgtcat
      121 tacccatgga tttactaaaa aaacggagaa aacaccaata agtgaactta aacatgcaaa
      181 agaacttaga gaggagtggt tcacaaatga tgattaaaaa attgattgaa gaaaagcaaa
      241 aagacaaaga ttttgcagaa gcatatgacc gtgaaggaag aaaactcgaa actgcggttg
      301 ctctttatca tgcacgggag gaagcaggcc taactcaagc ggagcttgca caaaaagcac
      361 atacaactca atctactatt gctcgaattg agagagggga taacgtatct ttcgataaac
      421 ttgatgatat cgctagggca ttaggcaaaa agctggtagt agaatttgct taaaagcgag
      481 ttgaaaatat acctgtaata ggctcctaac taatcaatta gtttagtggg cctttttgta
      541 ctaaatagat aaaagttgtc ctatagtgta aaagtcaata aattttatac aaaaattact
      601 ttaaagagac ataaagagaa aggtacaata aatgaaaacc ataaatctaa taaaagtatt
      661 tatagcatca ccaggtgata tagcagcaaa gaggaatgaa gtagaagaaa ccatatatag
      721 atggaatata gaaaatacag ataatacaaa agtaattttg atgccaattc gatgggaaac
      781 gaatgcgatt cctacttata taaataatag ctctgggcaa agtgttatta ataaacaaat
      841 tgtagaaaat agtgatatat tattagcgat ttttggccat aagttgggtt ctccagttga
      901 taattataga tcgggaacta tagcagaaat tgattacttt tataaaaata gcaaaggcga
      961 ggtaggggtt tttttcttag atgaaccagt tccacaagtt ttggctgaag agtatataga
     1021 agttaagaag tacagggact ctttagaaga taagggtttt tatggaacat atagcaaaga
     1081 caatatcaga aggtttctca caaaaaaagt gaattattta attgagaaag cacaaaataa
     1141 tttagaatca aaacgctttg atttagattc tatttcttac aagacacctt cagtaattaa
     1201 caaaaaagtt accggaactg ataaagattt tgatgacaac ttagatattt ttgatccaat
     1261 agaatttgac aatgatgaaa gattgctgct aatatatgcg tttatggatg aaaattctat
     1321 cttttcaact gataattcaa cttatagaaa agtagttcaa tgggaaagtg ataacaattt
     1381 aaggagttac ttgagtgata gagtatttga tgtactagaa aaattaagta aatcgggaat
     1441 tttaaaggaa gaatacgatt cttcttttga tgagtatcca tcacgctata ttttgatttc
     1501 agaggactat aagaaattta aaaggtattt tttaaaaaat gaaaaaaaga tatctgttat
     1561 tatggagaaa tacaaagctc caatacaggc tgatccattt tccaatttgg atgaatcaga
     1621 tttaccgttt taaattttaa atagataatt tcatttttta gtagcagagg tataaacttt
     1681 tttaatgttt gaattgaaaa gaaatgctaa taggtcgact ttatgtttct cttgtgcaat
     1741 cgtaaatgcg ggggaccata aaattgatct attatcgagt gtgtataaaa taaaattcaa
     1801 atcttattag tttacataag atatattatg caaagattaa gatgttaaac agcttaaaat
     1861 caaactgtta tatacgtgct aaatatttat gttaactata accttatttt attataaata
     1921 ttattacatg gtaagatgta aatatcttta agaaataaat ttgccggtag ttatttactc
     1981 cactattaaa tgagagccga attgttttac tatatctaaa tctttctttg tggatacaat
     2041 tacagtttgt cttgcatttc gtagtgtcaa atgattaatt tttctcacca tattatcgtc
     2101 gactatattg aagtttgatt tgataagtac acgattattt tgtaatctta attttgttgt
     2161 tcctaaacat aggctgggag caattggtat taaataatca atatcttcaa atttattaac
     2221 taatattgga ttatctccta aaaaaaaatt tcccttccct tccgacatgt atatttcaag
     2281 ttgataattg ttcattaaat gttctaagat aacttggatt ccactatcgc tatttccttt
     2341 taaaacgtgt aatgcgattt ctaattcttc accatttaaa tggctttttg catgttctac
     2401 agccatcatt tgagatccaa taatctgtct atacattatt gacttcatga ataatggaat
     2461 attgtcatta tcgtaaaaca atttttttat ataagggcta cgtattagtg ttaaggctat
     2521 atgactaagt agggctgctt cattacttgg ccactcattt gttttaatca tacgcagaaa
     2581 atctgaggtt gatttattcg aaagtaacaa ttctattaat gacttaaatt ttttcgatat
     2641 atcgttctca aatttggaat ataggtcttc tatttcattc cgattgatat aatttccttc
     2701 tgaatcttta gattcatata gatcaatttc ctgcattata tcagcagttt tttttattga
     2761 tacttctaaa ggttttttct ttagatcgat tatcaaacat ttaccttttg caacttcaaa
     2821 agggcgtata gaaaattgcg gaataaaatg ctgtcttttt gtgaactgct gtttttgttt
     2881 catattttat ctcccttcca ctaagcttta attcatagga aaatcactac tgaggtccct
     2941 tttaattcta tatactgttt gtctagttat attataattt ttagaaatag ttgagatagc
     3001 aactccattc tcaagatcct gaacaatgct cctataaaca aggcgacgtt gagggtcttt
     3061 tgtgtctgca ctgtataact tagggcgacc tttatagacg ccgtttgctt tagcaatctt
     3121 aataccttgt gcttggcgac gtttggattc ggtacgctct tgctcagcaa tcatggccaa
     3181 gatttgaatg attaaatctt tgatgaattt atctaaaaga ggatttccga ttgcttcagc
     3241 catgatcggt aaactagtga taatgagtct cacatttttc ttctttagat aattaactga
     3301 atcgataatc tcgtcatagt tccgcccaag tcgatcgatg gcttctacaa taaaaacatc
     3361 ttgctctcta acaaaatcta gagcttcctg aaaaatcggt cgatgagtaa tagttgctcc
     3421 agattgtttc tcgatgaaaa ttttttcggc accaaatttt ttcatttcct caatttgacg
     3481 ttgctcattt tgatcaatcg atgaaactcg gatatatgca attttcaata atttttacct
     3541 gctttctcca accatataaa atacacccta atcatacatt gaaaaacctt gtttttcaac
     3601 tatatttccg tgtctattta gggtatacct tatatagaca aaaactaatg atggaggaaa
     3661 tagtatgaga attttttgta agtgtctact gataacttat tctcaaatgt tgtaaggatt
     3721 ttattcattt gttaatatta aagaacggaa ctccaaaaaa gaccgtactc taatttgcag
     3781 tctctcactc ttttagagtg agagttttta ttagattttc aaattaatta gtaaagtgat
     3841 agaataaatt gatcgtacaa atcataagaa gaagcagggt gatagcatac ctatgcttct
     3901 ttttttatgt atttagctat ttcttcttta caatatcgaa catatgtttg tataatatga
     3961 aagaatggag tgttatttat gatggattac tcaaaagagc cagtaaatga ttacttttta
     4021 atcgatatga aaagctttta cgcaagtgtc gaatgtatag aaagaaactt agatccatta
     4081 acaacagaac ttgttgttat gagtcgtgct gacaacactg gatcagggtt gattttagct
     4141 tcttcaccag ctgcaaagaa gaagtacggt atttctaatg tcagcagacc acgggatcta
     4201 ccacagcctt ttcctaaaac gttacatgtc gttccaccac gcatgaatct ctatattaag
     4261 cgaaacatgc aggtaaacaa tattttcaga aggtatgtag cagacgaaga tttattgatc
     4321 tattcgatcg atgaatcgat cttaaaagtg accagatcac tgaatctttt tacgactgaa
     4381 ggaacaagaa gtcagcgcag gcagaaactt gctcaaatga tccaggaaca tattaaagaa
     4441 gaattaggat tgatcgctac tgtaggtgtc ggcgataatc cccttttggc taaactagcc
     4501 ttagacaatg aagcaaagca taatgaagga tttgttgcgg aatggacgta cgaaaacgtt
     4561 cctgaaaaag tatggaatat tcctgaaatg acggactttt ggggcattgg ttcgcgaatg
     4621 aaaaaaaggc tcaaccaaat ggggattttg agcattagag accttgctaa ctggaatcct
     4681 tatacgatta agaatagatt aggtgtgatt ggattacagc tgtatttcca tgctaacgga
     4741 atcgatcgaa cagatatagc gatcccacca gaaccgacaa aggaaaaatc ttatggtaat
     4801 tcacaagtgt taccacgaga ttatactaga agaaatgaaa tcgagctggt ggtaaaagaa
     4861 atggcggaac aagtagccat tcgtatcaga cagcataatt gtaaaacagg atgcgttcat
     4921 ttaaacattg gtacgtcgat cttagaaacg agaccaggat tttcacacca gatgaaaatc
     4981 cctatcacag ataatacaaa ggaattgcag aattattgtt ttttcttgtt tgataagtat
     5041 tatgaaggac aagaagtaag gcacgttgga ataacctata gcaagctaat ttatacagat
     5101 tcgcttcagc tggatttatt tagtgatccg caaaaacaaa tcgatgaaga aaacctggat
     5161 aagattattg ataagatcag acaaaaatat ggttttacag cgattgtaca tgcttcttct
     5221 atgttagaag gcgctcgtag tattactaga agcacgttgg ttggtggcca tgcaggagga
     5281 aatggggggc ttaaaaatga ttgattataa cgatcgagga cggttgaaat gggcaggatt
     5341 ctatttgtcc gatcacacgg aagagatcga tgcggacaat gagcaacgtg caaataataa
     5401 tttggcaaaa gaacaaatga cgactgaaga gatttcggaa gtattgaacg ctgcagtcat
     5461 aaaaaataga tcagtatgta ttcaaaaaga agaacgaaac gctgaaggat attatccaga
     5521 cgatattgtt ggaaagatct taggtagtga tgagctgggg atctacgttg gcaaatacaa
     5581 aattcactac gacgaaatac gtcatatcga attttaccag gataataaat ggagtcgatt
     5641 agaatgaaca ttatccagca atatgagcta aagtatataa cctttgatca gctatcagaa
     5701 gagatttggg gttatggcca acgtttgata aatgaggttg gtgttgagcg cttctctttc
     5761 tatgtagaag ctgcagcagg ttatcataat tttaggtttt atattttccc gttatttata
     5821 taaggt
//
LOCUS       DACERL010000114         5458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.119, whole genome shotgun sequence.
ACCESSION   DACERL010000114 DACERL010000000
VERSION     DACERL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.119"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(565..1605)
                     /locus_tag="IXF54_002651"
     CDS             complement(565..1605)
                     /locus_tag="IXF54_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1405864.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            3417..3635
                     /locus_tag="IXF54_002652"
                     /pseudo
     CDS             3417..3635
                     /locus_tag="IXF54_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3895..4149
                     /locus_tag="IXF54_002653"
     CDS             3895..4149
                     /locus_tag="IXF54_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1405865.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLIILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            4150..4467
                     /locus_tag="IXF54_002654"
     CDS             4150..4467
                     /locus_tag="IXF54_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1405866.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            5026..5458
                     /locus_tag="IXF54_002655"
                     /pseudo
     CDS             5026..5458
                     /locus_tag="IXF54_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1915 a          783 c          842 g         1918 t
ORIGIN      
        1 cgggtaatac cgtaacactg aaaggtaaac gcattaaggg attaaaggtt gtcttaatgc
       61 cacctgtgga cgtcgacgaa ggttaccagc cttccgagtc tcacagggcc tttctttttt
      121 ttgtattttc aattgtccaa atttatcgtc caaaagtgca gaacgaaaat taattcatca
      181 caagcgtagc agacaattaa atttaaaaca actaaattta tacaaaaaaa tgtaagcgaa
      241 taatattcgt attgcctgaa aagaagtaat atcaaggttt ttagcttttt taaaaaatga
      301 aattacctct cttcgcgtat tattataact aaaaaacagc aacatgcaaa cgtttgttct
      361 cttttttgtg ttggaaatta attattaact attaacactt atgacgtaaa aagctacgtt
      421 tgtgattttt atcataatat tttaactcat aaattctatt aaaatagcta tttctttgta
      481 acatttgtaa tataattaga ataataaaaa atttttaatt ttaatgctac tccatgaagc
      541 aacaatcaat ttgtcagtga aatttcactc taaccagtct ttcgtaatag tttttgggaa
      601 gctttcagaa ttcaaacgtt tttcacttga tactttagca gctattggtt tttcgataaa
      661 ccaatttttt acatagacaa aaatcaggtt ctggatattt tcaacttttt cggtttttaa
      721 tttttgatag gcttttaaca gtgtcgtgta taattcttgt tctgcattga cataataact
      781 atcgagttct tcaaaaatta ttgaaatacc actttctgtt tcggcttttt tctttgcatt
      841 atgaatagtc tggacagttt tttgagcttc actgtaactt gaagaaaatg tttttattaa
      901 atttaatact cgttcaggga taaacgtagc aatgctgtcg tttgtcataa ttgtctcaaa
      961 attgtctaac aacaattgat cttgtaggcg gtctttttca gtgtctatat tgtgtctatt
     1021 agtgtctaat atattattat taagttctgg tatgattttc ataccctctg atttatcaac
     1081 gttttggcac ggtatgattt tcataccctc ttgagcccca agggattcag cgtctgattt
     1141 ttcgtcgagg gtgggtatga ttttcatacc ctctgattta tcaacgtttt ccctattctc
     1201 tctatcaaat ttttcgagta agtagatatc attttctgag acttttaatt ctgctagata
     1261 tagtctatta ggattatttt tccctaactg tttgttaaat cccatctgct tttgtaataa
     1321 taaaccaaag gattccaact cttttttgat tgctatcgct ttttgttttt ggcagtttaa
     1381 tacgctacat agttcatttg tcgtataagt aaaataaaca ttaccttctt catcgatttg
     1441 ccgtttgctg attgcgatct ctaatcttgc ttttaaaagc atataggcaa tcttagttga
     1501 atctttcatt tttttgtatt cgtcagaaac aataaagact tttggaaatt gataaaataa
     1561 ttcgttgtaa actctatccg ccttaaaaaa attaaaatca gtcattgtag atcctctact
     1621 ttcacattgt tttatttatg taaaattacc tactcttttc gaaaatagca aagataaata
     1681 aaaaatgcaa acgcattaaa actgtttttt aattaaatac tacctaaaat cagaaataaa
     1741 atgaattatg tactaccttt aaagtcatta agaactactt taaataaata ttatttataa
     1801 ttaaatacta ccagaagaac gtatgaataa aatttagttt taaaattaaa gataattaag
     1861 tactaccagt gtcaaaacaa aaaataaatt aagaactacc ttatgcatat aaaacatact
     1921 accattacta gtaatatata ctaccttatt tcagtagtat atattatatt cttcttataa
     1981 gacaaggctt taggctagaa aacaatagat tatatactac taaaaaatac aataagtact
     2041 accaatgtca gatatatata ataattatgt actaccatta aaattaactt atttaataag
     2101 atactactgt gttaatctga aaaaaattac tttaaagtag ttacatacta cctgttaaat
     2161 tagatactac cattaatact taaattagat actacctata tttatattaa gtactaccgt
     2221 tctaggaata agataataaa aaaaaactac caaattgcaa ttaaatacta cttaaaatgt
     2281 aaaaaataag aactacccaa attaggtagt tcttaataag agaatatcag ataaatttgt
     2341 acaagtcata aaaaaattaa tcttaaattt ttaaatatat atacctgtta attataaact
     2401 actttattta ggtagtacat aattaacaga taaaactaca tgcttgtagt ttgtctagct
     2461 aatgagttgc atatgattta tataaaggtg tgcagcataa cttagttgaa gttaagagaa
     2521 tagaataaat attttttgtt ttgctaataa ttaaacacta cctaaatatg gtagtgttta
     2581 attatataat attcattttg ttacaaataa tcgtattata aaagcataac aattgggata
     2641 actatttttt agattattta agtactactt aaattaggta gtacttaaat tttggtttaa
     2701 ggtagtaggt aattaagaaa taatatatat cttcttttat aatagaaggg gagaggccta
     2761 gttaaaagca gcgacaatta ataacaatgt atttaatgct tcacaaaatc catttggtgg
     2821 acaatgcatt gttattagcg atgatctacc gttctaagta gttttttcta ttggttgcat
     2881 tcgtgactaa ttatggtata attatgagta acaaagaggt atttcattat atcatcaaac
     2941 cccatcagcg tgtaggctga tggggttttt tttacgtttc tatattatag aattaactgg
     3001 taggcttact gtcttttttg gcaattttgc aatcaagcca cttgcagatg tagtgtgcag
     3061 ttacctctgc aaatggcttg attgcaaaaa caaaatcaaa aaggtatttc attatatctc
     3121 ctcctcccct tgccagatta ggccggggta gcagacagca aaactattat aacataactg
     3181 aaatacctaa atgacatata cagatatgat aatttatgta ttgatttttc taacaaaagc
     3241 acatatttta atatatacta cctattttag gtagtatata tttttagata caatatcgat
     3301 cagttttgag gaaatgaaat tctagatatt ttttgctaca tagagttact ttcaatattt
     3361 tttaacgtaa ttaggcagaa gtcactcatc ctctataggt ttgtgatgaa attgctattg
     3421 atccatttaa gtaagagcaa aaaactggag gtttatcagg taattggtct aatggattta
     3481 agtatgcggt caccgtgtat agagttgctt atgagattca agaaaataaa gttattccag
     3541 tattgctata tggaacacat aacaattttt atgaattagc cgagaacact cgcaacttta
     3601 ttcgtgtaga tgaatcggct tcggttgagt tataacttta gctatatcaa tcaaccgacc
     3661 gacatatgtt aaatctatat ataataaaaa tacctactta aatggatgtt aacggaagtg
     3721 tatggtgagt agttcactcg tattttgata tcgtttgtat atgttttcag cgttctaagt
     3781 ggcttatttt gtccaataat aatatggagt ggaatccctt aagaaacacc gttggcaatt
     3841 gtcgttataa catgttataa tttatttatc aaaaactaaa agaagtgtga taacatggga
     3901 aaagaactaa aggttagaaa gatcggcaat agcgtgggag tgatccttcc aagctctctt
     3961 ggattaaaaa gcggagacac tatccaagcc aaacaagaag gcaacttaat catattagat
     4021 actactcaaa tagcaaaaga acacgatcgc aaattgattg aagaaagttt ccaagacttt
     4081 gagaaaggtt taacagtttc agaaattgaa atggtaaaag ctttcggaaa atacggctgg
     4141 agtgaataga tgaaagagta cacgctagtt tattctaaaa gtttccagac cagccttgaa
     4201 acgataatag aagaatggga aaatgagctt gctctttctc cagccagcat aaaaagattt
     4261 gtaggtgcga tccagaaggc tttagaacta accaaaaatt ttccaactat gcatgaggaa
     4321 gtttccgaaa tctatggaat ggatatccca acatatcgga tcgtgatcgg caaaagctat
     4381 gccttatttt atcggatcga ccatgaaaaa aaagagatac ttgtaggacg tattttccaa
     4441 tctaaacaaa tgaaactagg cttttaacaa taattaggca aaagtcgctc atcctctaca
     4501 ggtgggtgat taatgccgct cgatatggtt gttaaccgtc ggttactcga agattgatat
     4561 ataagactta gtaccttttt attttgctcc atgttatggc cgaaggtcta aagatacaat
     4621 cattagtcta gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg gtgaataaga
     4681 gacactgcta ttagtgaaaa aataagatat caagtacgct ctattcccaa aaatttcgtg
     4741 actttactca tgagaggttc aagtagtata tttttaatca aaattgacgt aacttttaat
     4801 aaatactacc taaataaggt agtatttatt tttgtgtttt aaaagaggaa gtccaatata
     4861 tctatgggaa acttcttaaa tattatgttg gtacatgaaa atatacatcc ctgctagact
     4921 atttacaaca ttttactttg tacgtattct tgttttaaaa ggaatgggat aaaatatagg
     4981 tacaataact atttgatgct aaatataatt aatggaggag agatagtgaa catacttaat
     5041 gaaaaaagat atgtatggtt tgctggtaaa aaatatatta ctactttcaa aagattatcc
     5101 gagttaatgc agcagactgt taaaagtgct atgcctgaag gatacaagtt taattttgtt
     5161 taatcttcag tatctactga aagtatctat ttttcaattc tttctgagag taggcttttt
     5221 cattttagaa taagttctca tgtaaagaaa actgatgttg atatatatac gttccgtaca
     5281 aataattata aaaatttaaa tgatttgagt agaagtatca gtgaatatct caaggggaat
     5341 agctatctca agattcgatt ttcacatata ttaattttac taccatattt gaatgagaat
     5401 ttcaccctaa ttcaagtaga taagtctttc attgcctcag gtaatcaagg aaatatga
//
LOCUS       DACERL010000115         5253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.120, whole genome shotgun sequence.
ACCESSION   DACERL010000115 DACERL010000000
VERSION     DACERL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.120"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            245..451
                     /locus_tag="IXF54_002656"
     CDS             245..451
                     /locus_tag="IXF54_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405867.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            647..814
                     /locus_tag="IXF54_002657"
     CDS             647..814
                     /locus_tag="IXF54_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1405868.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            840..1184
                     /locus_tag="IXF54_002658"
     CDS             840..1184
                     /locus_tag="IXF54_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1405869.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1189..1470
                     /locus_tag="IXF54_002659"
     CDS             1189..1470
                     /locus_tag="IXF54_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405870.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1573..1887
                     /locus_tag="IXF54_002660"
     CDS             1573..1887
                     /locus_tag="IXF54_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1405871.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1865..3559
                     /locus_tag="IXF54_002661"
     CDS             1865..3559
                     /locus_tag="IXF54_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1405872.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3579..4757
                     /locus_tag="IXF54_002662"
     CDS             3579..4757
                     /locus_tag="IXF54_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1405873.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4720..>5253
                     /locus_tag="IXF54_002663"
     CDS             4720..>5253
                     /locus_tag="IXF54_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1405874.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDE"
BASE COUNT         1865 a          751 c         1115 g         1522 t
ORIGIN      
        1 atttatagtg gtttatacca aattatcaaa gcaatgttat tttgttgctg tctattgttt
       61 tttaattatt cacacgataa ctaaaggtgg gtgaagagaa atgattccat taataatttc
      121 aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatgggc aaaataaaaa
      181 atagtagcag ccaagaataa ttttatagtg tcactgtggc ggaaagggta gacgctaagc
      241 atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagcgacatt
      301 aaatgcctat gacggttacg actaccgaaa aaagatcgtt aagaagctat acggtgctac
      361 gtacggcaat gtagtaagtg tgctatctgt acaccaccaa gcttcggtca ctgtggcgga
      421 agtagaagac gcagcggtaa atggcgagta gcctcgtgag agcctggtaa gttctcgtga
      481 gtggtgcaat cccactccag cgactttaag caaccgaggc atcggcggtt taaaaatata
      541 ggggtgcgca atttcgtacg cgttttgtgc atcgtgcaag ttactattac atattagatc
      601 actctttgag tggtcttttt atttttgcac aaaggaggaa acaacaatgt atagaccaca
      661 atacttagaa cagaagtatg aagtaatcac tgttcataaa ggtaatggcg aaaaagtata
      721 tgagtataga agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat
      781 tccattgtat ggcaaaagaa tagctaagca ttaaataaga ttgcgaaagg agacggaaca
      841 tgaccgagga attctataga tggctattac agttgacaag agaagatcgt ttggttaagt
      901 tctatcagtc tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg
      961 aatgccaaga gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa
     1021 aggaagtcaa ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg
     1081 ttgaacatca taatgaagtt catggcagat atcttacagc attagataaa caagagaaga
     1141 agatagaaag ctttgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat
     1201 gataatggca gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga
     1261 ttaattaaac gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac
     1321 tgttgtagta aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc
     1381 gaaagaataa aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc
     1441 gctgaagctt tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt
     1501 gggggtgatg aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa
     1561 aggagggcta aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc
     1621 cgctctttaa taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat
     1681 gttgatcgtt atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag
     1741 aacggcatta gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc
     1801 aacaaacaaa tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa
     1861 gcaaatgaat caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga
     1921 aggcactatc aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt
     1981 ctttactaga gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc
     2041 agcacaatac tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta
     2101 tggtgttcga tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg
     2161 acgtggtaat ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa
     2221 acacggtatt aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac
     2281 tagttttgat gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt
     2341 taaagctacg aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac
     2401 ggcaaatgct agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca
     2461 cgaaaatgaa gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga
     2521 ttatagagaa ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat
     2581 tatagaagaa tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga
     2641 attttctagt ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc
     2701 cgacatgtgg gaaaaagctt gtccaactat taattacaat gcagatttaa aacggaaaat
     2761 gtttcaagaa tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa
     2821 acgaatgaac agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct
     2881 gcatacgaaa gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt
     2941 tgctgataga cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta
     3001 ttttacacaa catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga
     3061 ggttatagat atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga
     3121 agctgattat attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat
     3181 cgctatggat atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac
     3241 tgtggaaatt gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga
     3301 cctttttatt aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat
     3361 gaatgtatat gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga
     3421 aactagaaaa acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat
     3481 ttatgattct gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt
     3541 tactcctcta gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg
     3601 taggatactt cacaaaaaaa gcaacggttc ctttagaaga atacttttgt aaattgcaag
     3661 ttgattttgt gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga
     3721 tgagtaaagc ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact
     3781 ataggctgaa tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga
     3841 tcaggagatt aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg
     3901 aaatatttat tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt
     3961 ctcaagtgca aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct
     4021 atataaaata cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg
     4081 ggaaactcat ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac
     4141 tgaaagggaa tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga
     4201 tgacatcaca atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac
     4261 aaaatgagta cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa
     4321 aaaacttaat agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc
     4381 tactaaaggg agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa
     4441 tcataccgat tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag
     4501 aatacttgtc agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca
     4561 aagatttggt tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag
     4621 gaagagaacg cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa
     4681 ctaaaaacaa tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt
     4741 ctagcagtaa agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct
     4801 agttggttct ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt
     4861 gatcaagaag aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt
     4921 gctattggga acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta
     4981 gccgctagtg ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat
     5041 gcacaattga tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt
     5101 acgattgctg accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga
     5161 ttcaatggca cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca
     5221 gaaacagctt tgagttatgg attggtcgat gaa
//
LOCUS       DACERL010000116         4899 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.121, whole genome shotgun sequence.
ACCESSION   DACERL010000116 DACERL010000000
VERSION     DACERL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.121"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(33..494)
                     /locus_tag="IXF54_002664"
     CDS             complement(33..494)
                     /locus_tag="IXF54_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405875.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            627..1001
                     /locus_tag="IXF54_002665"
     CDS             627..1001
                     /locus_tag="IXF54_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1405876.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            1055..3181
                     /locus_tag="IXF54_002666"
     CDS             1055..3181
                     /locus_tag="IXF54_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405877.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            3178..3798
                     /locus_tag="IXF54_002667"
     CDS             3178..3798
                     /locus_tag="IXF54_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405878.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            4491..4871
                     /locus_tag="IXF54_002668"
     CDS             4491..4871
                     /locus_tag="IXF54_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1405879.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
BASE COUNT         1882 a          694 c          657 g         1666 t
ORIGIN      
        1 aactatgcaa ttaaataact aactacttca tattagctta ataaaataat caaaataact
       61 agggggaaaa acattgtata aaaaaatttt tcatttttta aataagaata gtaaatataa
      121 aaataattta agatatctat tactattaat ccagatagag caaaccaagt aaatattcca
      181 tgtgtaaagc taaaaaaatt catttgaatc aattcaaata aacctaatat agtaacaaat
      241 atattaaaaa taatagctgt caataccagt aaattatttt ttttattttt tgaattatct
      301 tcatttaaaa tagaatttat gttagtacta taaaattcac aaattttttt tagtgtctct
      361 atatctggtg ttgttttgtt attttcccac tttgaaatag tttgacgtgt aacgttaaat
      421 tttcttgcca catattcttg agtataccct tttttcactc ttaattctct tagcttagat
      481 cctaaatcct ccattttttc tcctatgtaa acgattattt acataaaatt ttatcataaa
      541 aatactaacg cgccaaaaaa gttctttatt ttataaaaca cactaaaata aaataattct
      601 agtaaaaaag ataggagaaa taagttatga aaaaattttt atgtctttct gctttaatgg
      661 gtgtcttatt aactagtgga ggaatagcta gtgccacaga agctttaaat ctagatgtac
      721 ccgaagacca taccgccatt tacggaggag gaatggaagc tacagaagaa ggattttctt
      781 ctagtaaatt gagatatgct gctggcggcg gggatttcaa ctgtggggtt aatggattta
      841 aagtttatgc aaattactac catgctagag ctaaacatag tgcaacagct aaaaatggac
      901 gaggcggtca agtacgatca gttcagaaag ctggagtaag agcttatgca acatgcaatg
      961 ccacacttac tggaaatact ggttggtgga atgtttatta aaaaagagag tttaaactct
     1021 cttttttatt tacatttgaa aggaaattca aaatatgtta aaaaaaataa acgctgtttt
     1081 atcacttttt gtctgcatta caatactatt tttttctact aatgttataa atggtataaa
     1141 gacttctgaa gcgctatata aaaataccgc tgaaattcat atgcaatata aagatgatat
     1201 ggactttaac agttatataa acgctttgta tgaaatatct acacaagaaa atattaatat
     1261 ttctcaatat agttttactg ctgttaacca actaactatt cttgctacaa accctaacgc
     1321 taacaaacac tggaaatttt ctaaaaaaaa tatattagca agaaacgatt ctaaagtcca
     1381 ttatactaat aacaaaaatt ctaagtacca attaaaactt ccaaataatt ttttaaatat
     1441 aaatatttat cctttttctc atgtggaaaa tattggatta agtgaaatat tatatgtaca
     1501 aggaaactca aacaaattaa ttcctgtact gaaaaaatat ggaacaccta ctatttccaa
     1561 tatcaatcaa tcagatactt ttcaaattaa tactaatatt gttcttgtta tctgctatct
     1621 tagtatattt attgtaatta ctacaattat atttgctttt tcaaaaatga aagaaatcac
     1681 tctaaaaaaa atgttaggct ataactcttt tgatactata aaagattcta ttaaagagtt
     1741 aaacctctta ttttgttctt tgattataac tttcatttta tctatttttt tgagcatata
     1801 ttacagtaac gaaaattttt tcctatcatt tcttctatgt agtatttttc attttataat
     1861 tagtttttta tttatcatag ttactctaag tatctatcat ttatctagta taaaaaatta
     1921 tctaaaaaat tcacgaccac ttaagttaaa tctttgtata ttaaatatct gtctattctt
     1981 aagcatgatc cttctgttaa tagcttctac aaaagtaata aatacccata atgaagccga
     2041 aaataattct cttaaatatt gggaaagaac tactaattta tacaaaacaa atattactaa
     2101 tcaattaaat agaaacaaca ccgtagaaga aaataactac ttgaaaaaag ctagtaaatt
     2161 tgtttataaa atacagaaga attataaaac ttttattatc gcgccataca attacgcaac
     2221 tatccaagag aataataaag aattttttat tggacaaaaa gtttatccaa attttgaaga
     2281 ctatgttagt caacctgctg gtgcaggaat atgtgttgat ttaaactatc taaaatatta
     2341 caatcctatt tcttttgaag aatctactga cttaaattta ataaactctg atccactaac
     2401 tacttacatt ctagtaccta aaaaatataa agaatatgca aaaatgattg aaaaaaatta
     2461 tttagaagac atacagttta gacttgagga ctctcctcct caagctcctt ataaaaatcc
     2521 taatttgaaa aatttaaaaa taaaactaat atttgttaat aataaccaaa agtattttag
     2581 ttttaatacc ttatatggaa aggcaaaaga taactataca ataactgatc ccatagttag
     2641 agttattaat ccagaaataa cagaatcatt attttgggga aatatattga ctagtgatgg
     2701 tggattattt tttgatcaga aacatacttc aaaccaaaac ttttttaatc aaattaatcc
     2761 gtatataaaa gaatttaacc tagaaaatat cataaatttt tcttattctg tttttcaaga
     2821 aactggtgaa ttattatctc aaaaaaaagt aaataaatta aatgcatatc tacaatatct
     2881 tttaatatta ggattattta taataattaa ttttcaaatg atttttgtta gttttactat
     2941 aaattcaaaa aaaatagctt taaaaaaaat atttggatat tcaccaataa gtcaagtttt
     3001 agatttatgt ttactccctt ttacaataga gttatttgct actatgtgtt ttcaattgat
     3061 attttcaaaa aacacttact tgtattttat aatttttatg atctttttaa taaatctaat
     3121 tacttctatt attatttata aaaaaaatag ttttaaatat tttaaggaga atattttatg
     3181 atattatcag ttgagaattt caccctgtca attaataaaa aaaagatcaa acaaaattgg
     3241 aacttttcaa tatcagaaaa agattttatt atagtaacag gaaaaagtgg tagtggaaaa
     3301 actactctgt taaataattt aagcctaata gataaacaat ataccggtaa cttattctat
     3361 tttgaaaaga aagtttctaa acatgatata caacgtctac gaagaaatca tattagctat
     3421 ttatttcaaa actatgctct tcttgaaaat caaacagtac aatacaattt cgaattagca
     3481 aaaaagttta acagtaatat ttccaatgat catatttata atttattaac agatttttct
     3541 ttgccaaaag atatcttaaa agaaaaaatc tttctcttaa gcggtggaga acaacaaaga
     3601 atagcattaa ttagaaatat gttaaaacca tttgatatat tatttgcaga tgaacctaca
     3661 ggaaatttag ataaagaaaa ctcaactttt ataatgaact ttctgaaaaa tttaacagaa
     3721 aaagaaaaaa aagccgttgt actagtaaca catgacttaa atgttctaaa ctatggaact
     3781 aatattatag aattataatt tttacccgtg atgctagctg atttaaaact agcaccacgg
     3841 gtatttattt tatattccat ctctcctatc ccaaaattta aataatcact taataaaaag
     3901 gaggcaccct agtgatttta ttgaaaaaag agtatgaaaa attttatgat agacttattt
     3961 attctttgca attgccaaaa aaattaaacc gtcaattaca aaagacttat gaaactaaat
     4021 caactgaaga atgggagagt tattgctaga tttttggtag aagtaacaaa aagtggacat
     4081 attatcataa tgactcgccg aaagtctgat aaaaaattgg ctaaaaatat tgctgaatgt
     4141 ttaataaaac tatcagtttt agctaaatag ttagtagttt acacaaaaaa gcatttatgt
     4201 tttttgttgc aaagtaacaa tttcatcttc ggtgaaataa gcgaaacttt acaaaaagtg
     4261 gctagcattt gctagctttt tttgtattgt ggagcgtggc ttttgagggg gattcaaggg
     4321 ggagcttgcg cccctttgac aagcggttat ttgcactaaa tacgtaggta tttggctagc
     4381 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
     4441 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     4501 aaaatcaaaa tttggctagc aattcttcta aaaaatatac ctatcgatcc gagccaaaac
     4561 aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcaatcg gctgaagctt
     4621 tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac
     4681 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     4741 atatcaatca aattgctaag tggtgcaata cacaccaaca tgacgttgct tctggtgacg
     4801 ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac
     4861 aattaaggta agccgaacca cttcttgtag tcgtgcaat
//
LOCUS       DACERL010000117         4894 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.122, whole genome shotgun sequence.
ACCESSION   DACERL010000117 DACERL010000000
VERSION     DACERL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.122"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..642
                     /locus_tag="IXF54_002669"
     CDS             <1..642
                     /locus_tag="IXF54_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1405880.1"
                     /translation="LANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSY
                     GNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSH
                     QMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQI
                     NEENLDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            635..985
                     /locus_tag="IXF54_002670"
     CDS             635..985
                     /locus_tag="IXF54_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405881.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            982..1161
                     /locus_tag="IXF54_002671"
     CDS             982..1161
                     /locus_tag="IXF54_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405882.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            1173..1466
                     /locus_tag="IXF54_002672"
     CDS             1173..1466
                     /locus_tag="IXF54_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1405883.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            1692..2510
                     /locus_tag="IXF54_002673"
     CDS             1692..2510
                     /locus_tag="IXF54_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1405884.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            2529..2936
                     /locus_tag="IXF54_002674"
     CDS             2529..2936
                     /locus_tag="IXF54_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405885.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            3255..4340
                     /locus_tag="IXF54_002675"
     CDS             3255..4340
                     /locus_tag="IXF54_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1405886.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT         1901 a          707 c          832 g         1454 t
ORIGIN      
        1 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
       61 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
      121 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
      181 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
      241 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
      301 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
      361 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
      421 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
      481 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
      541 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
      601 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
      661 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
      721 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
      781 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
      841 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
      901 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
      961 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1021 tttgatcagc tatcagaaga gatttggggt tatggccaac ttatctctat gaatcctttt
     1081 ttgtttttac aaataggagt agatactaga ataatgcgtg tgttaaacta ttattgcaaa
     1141 cgcaataata aaagaaagta ggcttacgca ccatgtctat caatacattt gtttactcgc
     1201 acccgatcaa tgtttacatt atcaagaatt taggaatcac tgtagaacaa ttttgtgagt
     1261 tatatgccta cccacaagga acggttgcaa gctggataac ccgacaacgt agaataaaat
     1321 cattacctgc ttcatttgta tatgatctaa gtcttgcttc tagtatgaat atgaccgatg
     1381 tatacgaaaa gttattaata ctagaaaatg aatatgatca attcaattca aatcagcagc
     1441 gaaaagtaaa gaaacagata gactagaata gctattttcg aaaagcaata tttaaggtat
     1501 aagttatacc ttaaataata aaagtataag ttataccata aatatatatg gtataactta
     1561 tacttttatt attaaaaacc gctatatcca aacgttttta gatattatta tttaagttat
     1621 acttttaatg ttgaccattt ccgatatata agttatactt taattatcaa ttaaagattg
     1681 ggaggattta catggatact tttcaaggga ctacatttac agttggcaat tttaaaggag
     1741 gagtaggtaa aactaagata gtcagcatgc ttgcttatga caatgcaatg ataagaaata
     1801 gaaaaacgct tattattgat ttagaccctc aagcgaactt aacacaagtc atagcaagaa
     1861 ctttcggtat aaccgatatc aaaaccacta taacaaatgg tgtcgcaaat ggagaattag
     1921 caagtagtat tattaaggta aatgaaaatc ttgaccttct ggcatgtgac actagttttc
     1981 gatcattctc cgcttttgct aataaacatg aaaatgaatt agaccaagtt actgtcttga
     2041 aaaaattact tcttccaata aagaacaatt atcaagaaat atttattgat gtaccaccaa
     2101 ccattagcga attttccgat aatgctatgg cagcttcaga ctattcgata atttcatttc
     2161 aaacagctga agaatcctta gagggagtca acaaatatgt aaattatcaa aaattcatgg
     2221 tcgatagata cgatcttgat ttgcaaatta tagatattgt accttgtatg gtagaaccaa
     2281 atgatgattt tgatgaagaa atcctagaag aagcaattga aaaatacgga accgtggtat
     2341 ccaaaaatct cattcattat caaaaaagat tgagaaggta ttctaaaatt ggtatacatt
     2401 taagaaaata caaaaatgga aattttgatc aatgggatta taaagcacac caagtattta
     2461 taaatatttt ggcagaacta gatgctagac aagagttttt aaataaataa taaggagttg
     2521 cataagtaat gcctaaagat tttgatattc ctaaaatgat ggagaatggt gaatttgagc
     2581 cattattacc caataacaaa cgccaagatg tgaaaataag cggtgaatat aactttagca
     2641 atagtgcatt gagtaacaca agaaatatta cattgaaaaa aagaggccga ccagtttcaa
     2701 ttcctgataa aagaaacaag gtatcagttc ctaaaaagat ctctccggca ttaaatagca
     2761 aactctcaat tttacaagac tatatgacgg agttacaaag tgaaaaaggg agaatttctt
     2821 tcgaaaaaat tgttaataca ttagcagact catatatcca gcatagacta ggagtagcaa
     2881 aagaagaaca cataagagaa gaaatacaag aagagtttga aaaattacca aaataatttt
     2941 tttcatgcgg tattcaatta aagtataagt tatactagat aaatgaatat taaagtataa
     3001 gttatacatt aaatatttaa ggtataactt aataaacaac gtgtaaattt gcgattaaat
     3061 caacattata aaattattat ttaaagtata agttatacat tatttgtatt taaagtataa
     3121 cttatactta tttttttaat atataactta taccttaaat actgaaagtg ttttattcac
     3181 ccttaataca tggtatagtt cacttgttga aaatcctgaa tatatacaag tttctaagaa
     3241 gaaggagaaa tttaatgtct acagaaaatc aatctcaaaa aatgataatt aaaaattggg
     3301 gatataaagc aacgaatgtt tatattgaaa aatattataa atttcctagt tttttaattc
     3361 agaattctaa atactctaaa atgaaagaca gctcaaaaat agcctatatg ctttttaaaa
     3421 atgaatttcg tagagcctta catgaaaaat ggatcgacga tgatggtcta ttatatttgg
     3481 aatttacaca agcagaccta atggacaaat taaattgcta ccaaggcaaa gtaaaaacca
     3541 ttttaaacga gttagaaaca tataatttga tttcagtcgt aaaaggagaa tttaacccaa
     3601 aaacgaaaaa aaatgaaaaa aataaatact atttacttca acctgaaata agtgccgatg
     3661 atctttttat tcaaaattct ttcgatgacg atttaagcga aactattgat aaatcaacct
     3721 cttctcggaa tgcggaaatc gcacgtcgag aggaaactgc taaaaccctt gataacactg
     3781 gaaatgcgga aatcgcacgt cgagaaaaaa cagctgaaac ccttgataac actggaaatg
     3841 cggaaatcgc acaagattat tatattaata aatctttaga cactaaaaga cacttaaaag
     3901 acactgaaac agaccaatta caaaatcaag ttttgctaga taacttcgtt gaaatcatga
     3961 aagaagacag cataaacaca tttgtaccag aaaaggtatt aagcttgata aaaacatttt
     4021 ctaacactta cgaagaagcc caacaaaccg tcaccacaat acacaacgca aaacataaag
     4081 ccgaggaact tgagaagacg gtagttgtgt atgaagaact tgaatcttac ggaattgatg
     4141 cggataaagg attatatatg acgttgctga aagcttatca aaaacaaaag actgagaaag
     4201 taaataattt gcagaatttg atttttgtgt atgtaaaaaa ctggtttatt gaaaaagcta
     4261 ttgcggcaaa gcttgcaaac gaacaaagag aaacatttaa cgaattacca acagttagta
     4321 cggaaaattg gctagagtag ttgataaaaa gcgaacacta taaaaatgat caattttaaa
     4381 ttagaacttt tttacaattt taattttatt acaaatgtta caaataaata gttattattc
     4441 cggagttttt agattaaaag tttatgaaaa caatcacaaa cgtaggtttt aatgtcataa
     4501 tcgttagata ttttagttat ttttcattca aaaaaacagg gaactagtgt ttgcatatgt
     4561 atttttttga gttataataa ttcatgaaga gaggtaattc acttatttac ccaagctgaa
     4621 aagcctgata ttacttcttt tcaggcagtg ctgctaatat tcaattacac ttttttgtat
     4681 aaatttagtt gttttaaatt taattgtctg ctatgcttgt gatgaattga ttttcgttct
     4741 gcacttttgg acgataaatt tggacaattg aaaatacaaa aaaaaagaaa ggccctgtga
     4801 gactcggaag gctaccaacc ttcgtcgacg tccacaggtg gcattaagat cactccctta
     4861 atgcgtttac ctttcagtgt tacggtatta cccg
//
LOCUS       DACERL010000118         4863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.123, whole genome shotgun sequence.
ACCESSION   DACERL010000118 DACERL010000000
VERSION     DACERL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.123"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..1220)
                     /locus_tag="IXF54_002676"
     CDS             complement(<1..1220)
                     /locus_tag="IXF54_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1405887.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1281..1856
                     /locus_tag="IXF54_002677"
                     /pseudo
     CDS             1281..1856
                     /locus_tag="IXF54_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2037..3230)
                     /locus_tag="IXF54_002678"
     CDS             complement(2037..3230)
                     /locus_tag="IXF54_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1405888.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3314..3517)
                     /locus_tag="IXF54_002679"
     CDS             complement(3314..3517)
                     /locus_tag="IXF54_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ1405889.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3774..4016
                     /locus_tag="IXF54_002680"
     CDS             3774..4016
                     /locus_tag="IXF54_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405890.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3938..4171)
                     /locus_tag="IXF54_002681"
     CDS             complement(3938..4171)
                     /locus_tag="IXF54_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1405891.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4178..4609)
                     /locus_tag="IXF54_002682"
     CDS             complement(4178..4609)
                     /locus_tag="IXF54_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1405892.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1004 g         1424 t
ORIGIN      
        1 tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag ttctgcattt
       61 tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt ttttttagag
      121 gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc cttgttcgct
      181 atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg actctgatcg
      241 gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat aaaaacaacc
      301 agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg gattttctcc
      361 gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga tacaatattt
      421 gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc taatatttct
      481 aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga aaacagtaga
      541 cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt tatttgatac
      601 agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa cataatggaa
      661 aaaaaaaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag ataattagat
      721 gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa taaaatacaa
      781 atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa tctcgtctgt
      841 ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc aaaaaaactt
      901 gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag aaaaaagctg
      961 agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag gtttttcgta
     1021 gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct cactttaggg
     1081 ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt ttttactagc
     1141 agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa
     1201 agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg tacacatatt
     1261 tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac cagtttcaca
     1321 gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg cagctcactt
     1381 ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac gatgttttct
     1441 ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa caaatacgta
     1501 aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt gcagtagaga
     1561 tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat ttatctgaac
     1621 aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta aatgtcgata
     1681 aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa attttatata
     1741 ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca gaagataaat
     1801 tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct tactttttct
     1861 taaaaattat atgggatttt agattatctt tttagaagtt cttgaatgtc ggtatagccc
     1921 gtgtttccgg gcttttccgg catttttgat attgttagat acttacaaat gttcttatta
     1981 cctctcacca tttccccctc aaaagtagta aatggagtag taaaaccggc cttgtgtcag
     2041 gctgccagcc gttccatttc tgcccttgcg gaatcgaatg tggcgtgtgc ataatagttc
     2101 agcgtcatgg tgatattgga atgtcccata atgtactgca aggctttggg gttcattccc
     2161 gcattggcta accgggtgca gaatgtatga cgcatagcat ggggcgtcgt cgttttcggc
     2221 aacgcttctt catggctctt gttgtacttc ttcacaagcc tgccaaacat cccgccataa
     2281 tctaccgccg tcatgggata gccgttctgt ttgagaaaca ggaaattggc atacccgtca
     2341 ataatgaacg gctttgcacc tttgcggttt tttatcactc gctggaacgc ctgatacact
     2401 tcctcattca tggggatttt tctgacgccg ctttgggtct tgggttcgtc aatatagtaa
     2461 ccgacgccgg tatttctcaa aagctggtgg ctcacgatga ttacccggtt ttcaaaatcc
     2521 aaatctttgt ccgtcagtcc gcacagttcc gaaatgcgaa gccctgtccc cagcagaata
     2581 acgattgcgt cataatgctt ttggtaaacc ttatcacctt gaataaagga cagaaaactt
     2641 tcttcctgtg ctggtgtaag aggcaccttt ggttccgtat cgtcgtccag aacgtcacta
     2701 agctggaagt caaacgggtt ttttcggatg caatcgtcct gtatggcggt gtaaaaagcc
     2761 gctttcaggg aacgcttgtc gttattgatg gtcttataag aaaggccctt ttctttcatc
     2821 cgaagcgccc attcctttgc gtcggacagc ttcacactgt caatcgggca gctccccagc
     2881 cgatcttgtt ccagaatacg catcagccgt tcccggccca gtttggtgtt gtgcttcaca
     2941 ttgccccggt gcctgatctg cttggcgtag agctggcaga cggtcatttt cttgccgatg
     3001 gtgtctatcc cgtcgtcaag gtctttctgg atttctttct ccttttcccg gagtgaaata
     3061 tcctcccgtt tacctgccgg ggtcttatcc gtaggcacta acttccatga gtaaacaaac
     3121 tgcggttttc caaaggtatc aacatatttg taagcatatc gtccgtcttt tctctggctc
     3181 tctccagacc gcaaaacgcg atttttgctg tctcgtcttt tttctgacat ctcgtcatgc
     3241 tcctttccat gacggaaaga gccgcatata tgcttataaa ttattataac atatctgcgg
     3301 ccctttttca ctattagatt gtgtccaaag tgtcaattat tttctcaaac tgcttgcgtt
     3361 tgatctggat gcggttgcca ttcataatga cccagccaga ggccggattt tcctcggcca
     3421 gcttacgcaa tttgttttct ccgatacgga aatatttaga cgcttcctca atcgtcagcg
     3481 tgtatttttc ccaaataggc acatcatggt tcataatgca gccccctttc acaaattcaa
     3541 aggtttgtgt gatttagggg catagacgga cggctgctgg cacagctcca cgggactttc
     3601 acccccgcgt ggttctcacg cggccctacc cattgcctgc gacgctttta acgctcggac
     3661 tgtggctata agggagtatc atagtcctgc ctgtacgtcg tcgcccgcaa gccgccacgc
     3721 tcacattgcg gcgtggtgtt cctcgctccg cttttcccgt agggaaaagt atcatggcgc
     3781 accagccgtc cggctcggca cagacagaat gtgtccgtat gccctatgat gcgccgccgt
     3841 tatcttgcgg gcatatttat caaggtgcga tcaggccacc gggaaaacac cgaaacgggg
     3901 aaagggtaag ccgtttcatg ctgccggggc cggttcgtca gttcgtgacg atagcgtgga
     3961 tgagcttgat ttccagacgg cgcttcatgg cctcgtccag acacacatgc ggatagccgt
     4021 tttcgtcata gagcgtccgg gtacacagct tgttcatgta accgctgtaa tactgcaaaa
     4081 cgcgctctac ggctccggca tccccagcgc gggcggcctc gatcaccgaa aggggcaaaa
     4141 gctcaatgcc cttgtagttc gggttcttca tccgcgtcta acctcccttc ggcatcaacg
     4201 cctttagctg tttccgcagc tccgataagg tatttgtcct gtgccgctga acggcactcc
     4261 gggacattcc cacaaggccg ccgatctcac catctgccat atccagaaca cagtgcagga
     4321 ttaaaatact ctgctccatg ctgggcagcg cggcaaaggc ttcggcaaca agctcattgt
     4381 tgatatgtag gtcatagcca tgcgacgaaa acgtaaagct gtcgctgggg tagtgatcta
     4441 ctgtacagag cttgtccaaa tctgcctgtg acaaagagct tagtgacact tcacggtcac
     4501 ggcggcgctt catttcagcc aagtagcttt tggcctcgtt acgcagtacc gtcttgcaaa
     4561 aagcatcgaa catgcaccgc gcttcatagt cgcgggggat agcatccatc ttctcacccc
     4621 ctttctgtgg gggacttagg tggttctctc cctttccgct aacaagactt tgacaaggcc
     4681 gccagctacc cgctaaatac ctcctttttg aaaatttctc aaaaaatttt tttggggaca
     4741 gccgggccgc aaaagacgac aaaaatcgcc cggcaggtcg caaacctacc gggcgagaaa
     4801 atgctttgct atgctgctga tttacatggt atagtacata ctcataaccg gaatagccct
     4861 gtg
//
LOCUS       DACERL010000119         4782 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.124, whole genome shotgun sequence.
ACCESSION   DACERL010000119 DACERL010000000
VERSION     DACERL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.124"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(207..1169)
                     /locus_tag="IXF54_002683"
     CDS             complement(207..1169)
                     /locus_tag="IXF54_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1405893.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXF54_002684"
     CDS             complement(1171..2610)
                     /locus_tag="IXF54_002684"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1405894.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IXF54_002685"
     CDS             2795..4741
                     /locus_tag="IXF54_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1405895.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1520 a          819 c          790 g         1653 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttgtctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtctattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatgtatttc cctgagtaaa gatggaattt tctttccaaa at
//
LOCUS       DACERL010000120         4758 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.125, whole genome shotgun sequence.
ACCESSION   DACERL010000120 DACERL010000000
VERSION     DACERL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.125"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..391
                     /locus_tag="IXF54_002686"
     CDS             <1..391
                     /locus_tag="IXF54_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1405896.1"
                     /translation="KNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEI
                     TGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNG
                     FQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            406..1158
                     /locus_tag="IXF54_002687"
     CDS             406..1158
                     /locus_tag="IXF54_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1405897.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1174..1929
                     /locus_tag="IXF54_002688"
     CDS             1174..1929
                     /locus_tag="IXF54_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405898.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            1942..2202
                     /locus_tag="IXF54_002689"
     CDS             1942..2202
                     /locus_tag="IXF54_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405899.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2199..4289
                     /locus_tag="IXF54_002690"
     CDS             2199..4289
                     /locus_tag="IXF54_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1405900.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            4316..>4758
                     /locus_tag="IXF54_002691"
     CDS             4316..>4758
                     /locus_tag="IXF54_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405901.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKY"
BASE COUNT         1768 a          757 c          929 g         1304 t
ORIGIN      
        1 taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag acggtgtcaa
       61 agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag ataaagaagg
      121 tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa caaaagcgcc
      181 tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag attcatacaa
      241 agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa tgaccggtag
      301 caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag gggcactttc
      361 tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat tatcaggaag
      421 cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact ttcagctgta
      481 ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca aaatcagcag
      541 attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt ggatgttgct
      601 aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc tttaaaacta
      661 ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat tacggaagga
      721 actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca tagtggactt
      781 tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa gttttatatc
      841 aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt gcaaaaagac
      901 gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga tcgagtaacg
      961 ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta tgggaagcga
     1021 gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa attatcttgg
     1081 aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc gctatttgtt
     1141 tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg gctatttgtt
     1201 tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc agacagacaa
     1261 gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac gattcaaagc
     1321 agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa aattgctgaa
     1381 gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc agaacaatta
     1441 gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc tgtttatgat
     1501 gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca aattgaatcc
     1561 caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt ttctaatgga
     1621 gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga agtacaggca
     1681 aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc
     1741 gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt aaaggatgtg
     1801 tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa aatcgtttct
     1861 actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca cttgttttct
     1921 agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa tttttcttgt
     1981 ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat tttgccaagc
     2041 taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt
     2101 atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga
     2161 atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga
     2221 caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa
     2281 aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg
     2341 taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa
     2401 acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg
     2461 actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaagaat
     2521 acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc aatattgact
     2581 atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt ctatctaata
     2641 ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat acaaaaactc
     2701 attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa
     2761 cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga
     2821 atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat
     2881 ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat
     2941 atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat acggttcttt
     3001 cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct
     3061 ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga
     3121 agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg
     3181 gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc
     3241 ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataaaatgt
     3301 tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg
     3361 atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtaaatt
     3421 acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga
     3481 cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc
     3541 gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg
     3601 ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg
     3661 atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg
     3721 gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct cctttaaacg
     3781 caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc gtagacgctg
     3841 cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt ttgacatata
     3901 aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact tctgaaggca
     3961 cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct gacttcttaa
     4021 gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa actccattac
     4081 aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt tctaataaaa
     4141 caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt cctaaaacag
     4201 gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg ttagcaactt
     4261 ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga ataaaatgaa
     4321 aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag tcggatgtag
     4381 taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag gaactatgtg
     4441 gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga ctacctggga
     4501 atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga ggcaaaaaga
     4561 ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg aatttataaa
     4621 caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa aaaaaataat
     4681 ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc acgatggatc
     4741 acaatccaat aaatataa
//
LOCUS       DACERL010000121         4685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.126, whole genome shotgun sequence.
ACCESSION   DACERL010000121 DACERL010000000
VERSION     DACERL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.126"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..533)
                     /locus_tag="IXF54_002692"
     CDS             complement(<1..533)
                     /locus_tag="IXF54_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1405902.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGM"
     gene            complement(750..2561)
                     /gene="ltrA"
                     /locus_tag="IXF54_002693"
     CDS             complement(750..2561)
                     /gene="ltrA"
                     /locus_tag="IXF54_002693"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1405903.1"
                     /translation="MSTKLRYFEYYDMQKTFDWLYQRSKHNAMTGVNLYDLIISERNI
                     LLAYRMIKANTGSKTAGVDGRTISDFKIKDRASFIREIRSTLQNFTPQGVKRVEIPKV
                     NGKIRPLGIPTMRDRLIQQMFKQVLEPICEAKFYNHSYGFRPNRSTHDAIARCNFLMN
                     RSTFHYVVDVDIKAFFDNVNHKKLLNQLYTIGIKDRRVLAIIGKMLKTPIEGLGVQGK
                     GTPQGAILSPLLSNVVLNDLDQWVSSQWENIPTHFPYKSNWVAKVQLRKSSKLKEVYI
                     VRYADDFKIFAKNYKDAWKIFQAVKGYLNHHLKLEISPEKSKVTNLRKRSSEFLGFEL
                     RAIQNKGKYVASTHVSKKNKARIKQKVKEHLKILQKSPSWQNVKAYNSYILGLHNYYR
                     VATCVNIDFSKIAYSLSYTHFNRLKIVGKYEVPLRPPPVYRKFYKNNLRTYKVAGLYL
                     YPLADIQWKLKLAFSQITSDYTPQGRAKIYHNLNSSLSNEIQKLQYSDHTGFSLEYED
                     NRISKYSMQNGKCAITGKFLTAQEVHCHHILPRRLGGSDEFSNLIVVHEWIHILIHAN
                     HQSTIEQYKKLLNLTKTQIEKVNKYREKCNLTKIKYQ"
     gene            complement(3235..4632)
                     /locus_tag="IXF54_002694"
     CDS             complement(3235..4632)
                     /locus_tag="IXF54_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1405904.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNGATRFTISA"
BASE COUNT         1279 a          958 c          752 g         1696 t
ORIGIN      
        1 ttcatgccga tcaaccaatc ggcgatctgg cgtgcttgtg cttcttcaaa ttttgggtag
       61 gtctcttctg catctcgtaa ctgctggaat ccctgataaa tggcttcttt ttctaaggag
      121 ttgatccaaa gtcttttaaa tatctttcca tgatcggcgc ctgcttgttg aatgatcgtc
      181 caagcgatca gttccccttc gcgatcgcta tctgtcgcaa taataatggt gtcggccgat
      241 tgaagttgct gtttcaccac gttgaattgc tttttcttat ctttaggaac atgatagtga
      301 taatgtgtag ggaaaatagg taaatgttct aatgaccatt gtttccaatt ttcatcgtac
      361 atatcgggcg aatctaactc gactaaatga ccaaaaccat acgtaatcac agtttcacca
      421 ggaaacaatc gatcttggat ctcaaaatac ccgtcttttc gggtggcttt agaaaagcta
      481 tcggcataag ccttcgcttg tgacggtttt tctgctaaaa tgacagtttt catcgtgacg
      541 atctcactcc ctttcttgtt gtctgccttg ttgaaacaac atcgcacgtt tttgctttct
      601 aatttcttgt tgctggatct catgcttcaa ttgatatagt gataggtaac cctttgagct
      661 ttattttctt agctattaaa ggtctctcta ggttttcccc cactccacac cgtacgtgca
      721 tctttcaacg catacggcgt tccaacttgt tattgatatt taattttagt taaattacat
      781 ttttcacgat atttattcac tttctcaatc tgtgtttttg ttagattaag aagcttttta
      841 tactgttcaa tcgttgattg atggttcgca tgaataagaa tatgaatcca ttcatgaacc
      901 acaatcaaat tagaaaattc atcgctacct cctaatcttc taggcaaaat atgatgacag
      961 tgaacttctt gtgccgttaa aaattttcct gtaatggcac acttcccatt ttgcatagag
     1021 tattttgaaa ttctattatc ttcatattct aaggaaaaac ctgtatgatc cgaatattga
     1081 agtttttgaa tttcattaga aagtgaagaa ttaagattat gatatatctt tgcccttcct
     1141 tgtggggtat aatcactggt tatttgactg aatgcaagtt taagtttcca ttgaatatct
     1201 gccaaaggat aaaggtataa accagctact ttgtaagttc tcaaattgtt tttatagaat
     1261 ttacgatata cgggaggtgg ccgcaaggga acttcatatt tccctacaat ttttaaacga
     1321 ttgaagtgag tataagagag agaataggca atttttgaaa agtcaatatt cacacaagtg
     1381 gctactctgt aatagttgtg tagtcctaga atataagagt tataagcttt tacattctgc
     1441 caactcggac ttttctgtag tatttttaag tgttctttca ctttttgttt aattcttgcc
     1501 ttatttttct tggaaacgtg tgtgctcgcc acatatttcc ctttattttg tattgctctc
     1561 aattcgaagc ctaaaaattc tgaacttcgt ttcctcagat tagtgacttt ggatttctcc
     1621 ggtgaaatct ctagcttgag gtggtgattc aaatatcctt taactgcttg aaatattttc
     1681 caagcatctt tataattttt cgcaaagatt ttgaaatcat ccgcataacg tacgatatac
     1741 acctctttga gtttagaact ctttcgaagt tgtactttcg ctacccaatt actcttgtaa
     1801 ggaaaatgtg taggaatatt ttcccattga ctacttaccc actgatctaa atcattcaat
     1861 actacattgg aaagaagggg agaaagaata gcaccttgag gtgttccctt tccttgtact
     1921 cccaaacctt caataggagt tttaagcatt tttcctataa tggcgagaac tctcctatct
     1981 tttattccaa tagtgtaaag ttgatttagg agctttttgt ggttcacatt atcaaagaaa
     2041 gctttaatat ctacatctac gacatagtga aaagtcgatc gattcatcaa aaaattacac
     2101 cttgcgatgg catcatgagt cgatctgttg ggacgaaacc catatgaatg attgtaaaat
     2161 tttgcttcac aaatcggttc tagtacttgc ttaaacatct gttgtatcaa acgatctcgc
     2221 atagtaggta tacctaaagg tcgtattttt ccattcactt taggaatttc tactcttttc
     2281 acaccttgag gtgtgaagtt ttgcaaggtt gatctgattt ctctgataaa actcgctcta
     2341 tctttaattt taaaatcaga aattgtccgt ccatctacac cagcagtttt tgaacctgta
     2401 tttgctttta tcatccgata ggctaataaa atatttcttt ctgaaatgat gagatcgtat
     2461 agattaactc ctgtcattgc attatgttta ctgcgctggt agagccaatc aaaagtcttt
     2521 tgcatatcgt aatattcaaa atatcgtaat ttcgtactca ccgatagatt atttctccta
     2581 actattgtta gtcctatcct cttactcgac cctgtgagtt tcatttaact gttattaaaa
     2641 tttcaaagaa caagtcttga ggctatccct ctattcattt tcatgaattt caacggtact
     2701 gtgcctctac tctcacgaga attaagaaat ttcagttctt accttatatt caacactccg
     2761 ctaacagacg tttgtttaag tgtttctcgc tttccgcgtt ccaatgattc tagctgtaca
     2821 tcaatcctta ggtgcttact atgagcctga acatttatca catctttgtt tgcgattccg
     2881 aatttcatgg gaacgacgct tacttttcgg taatgttctt tgcacacgca atcctctatc
     2941 tttcgaatag agtcgactta tagacccgta cattcgcaag ttcgtcagtt tctcaacaga
     3001 aacattctca ccatagattg tttatagcat tccgacatat ccgcaaccat atagaaacgc
     3061 atcctactta ggttttgttc tgtcgacttc acctggcttc acacctccat gtactgttac
     3121 acatgaacgc atgcaggagt atcaaatcag ttgtttcagc caacatgacg gctttcattc
     3181 caactgtcaa atcatcagtt gtaatttact tcacgtaaat tctcactttt cgtgctacgc
     3241 acttatagtg aaacgcgtcg caccgttcca atcctttttc acttcaggta caaattcatg
     3301 ttcatgattt ttatagcctt tgtagtattg ttctgcttta taagagaaaa gaatctgagc
     3361 attaatatcc cctttttctt gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc
     3421 acaacctttt aattcttttg cgacttctct acaaatatct ttcaaattaa tagcttctat
     3481 tgaatcgtta acagaaagct gctggtcaaa agacgcattg actttgtgag cggaaaccag
     3541 ttggttcgtt aattgcagat tgttttctgt cttgtgtatg gccataatag cttctggtgt
     3601 gaccttttct tcttttgcga cttcttcata aatgattttg tattcttttg gcacttgcca
     3661 atcattagcg attcctcgtt cacaatgtac tttctgattc gtaaatggat cgactgcgcc
     3721 cattaattgt tccttttcgt agaaaatatg tcctgcacga tcattatcca cacaaatctc
     3781 aatcgattga ggaaaggctt gtttctttct caattcagat actgcctcac caaaataatg
     3841 actgatcaca tggtgtttta acccttccat agagactaac cacgtatcgt ttaactgatc
     3901 acgattcagt gcagcgtagc taagtaaatc aatactactc tcaaaaaatt ttaactgctt
     3961 agggtcaccg atctttaggt taaagccatg attcgctgtc gaatttttat ctatatgctt
     4021 atatgttcct ctctcaccaa aacgttcata atcaataaac gttccttgat aactcgcccc
     4081 cacagtctct tttgtctgtg gatctttcca taaaaaggca actgcatgat ctaaacgatt
     4141 gtctcgaact aaaaaatttt gccgttcatg ttttggcttt agttcagtaa tcaatccttc
     4201 tttttctgca taatcaatca gccactctgg caattttcgt tcttccttca gataattcct
     4261 tcctttttct gtaagtggtt gataatcaac ggggtgatct ttgtactttt ctgtctcaaa
     4321 atgaagattt tctactcgtt ctgttttggc ttcattcttt agaataaaat cagtggctgc
     4381 tttaaattgt tgcttagggt cttggatctc tttgtctata aaaaaaagtt tagctaattc
     4441 aataatgtcg ccactaatat tttgactatt ccaataaaaa cgttgttttc ttcgatcgaa
     4501 aacaaacgaa tcatggtctt ctaagcgata gtcacgtcct ttattgacta cagccattcc
     4561 attgtttcgt gcaaaatcca caatatccat ttgcttgcat cgatcaatga gactcatttt
     4621 tttctctgtc attttcaaac ctcccaaaag aaaaaagact atccattttt cagatagtct
     4681 ttctt
//
LOCUS       DACERL010000122         4633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.127, whole genome shotgun sequence.
ACCESSION   DACERL010000122 DACERL010000000
VERSION     DACERL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.127"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..462
                     /locus_tag="IXF54_002695"
     CDS             <1..462
                     /locus_tag="IXF54_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405905.1"
                     /translation="PGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQ
                     RLLNAYETPEYYFADYGEAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAV
                     THEDIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
     gene            486..2612
                     /locus_tag="IXF54_002696"
     CDS             486..2612
                     /locus_tag="IXF54_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405906.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            2775..3221
                     /locus_tag="IXF54_002697"
     CDS             2775..3221
                     /locus_tag="IXF54_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405907.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            3223..3360
                     /locus_tag="IXF54_002698"
     CDS             3223..3360
                     /locus_tag="IXF54_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1405908.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            3398..3691
                     /locus_tag="IXF54_002699"
     CDS             3398..3691
                     /locus_tag="IXF54_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405909.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            3688..3912
                     /locus_tag="IXF54_002700"
     CDS             3688..3912
                     /locus_tag="IXF54_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1405910.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3909..>4633
                     /locus_tag="IXF54_002701"
     CDS             3909..>4633
                     /locus_tag="IXF54_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1405911.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNYAHIDQFGEIENA"
BASE COUNT         1625 a          762 c          962 g         1284 t
ORIGIN      
        1 ccaggttgtt tttttggttc atgggaagat gggaaacttg ctaggtttga acaatcaacg
       61 gtacaagtat ataaagattt tactgttaga ggtactaaaa actcaactgt accaacagaa
      121 cattatggac aacgactatt gaacgcttat gaaactccag aatattattt cgctgattat
      181 ggggaagccg ttacaggtga caatggtaaa gttcgtgttg atattgaccc catgtttgct
      241 gaaacagtaa atctaagtcg gtatatgaca catgtgacac ctacagaact agttttgtgt
      301 gctgttactc atgaagatat tgaccatttc atcattgaaa ctagtaagcc aaacgtatta
      361 gttagatgga atttagtggc acaccgtcta gggtatgaag atattagatt aaaagaggat
      421 acagcatatg atagcacagt gcttgaccaa aaacgttttt aaaacgaaga caaggaggta
      481 tataaatggc tagcagttta tataatttgg ctttagattt cagcaaagaa ttaaactaca
      541 ccaaagctat tatggctcgt caaggtgata aagggattac ggtgacggtt aaaccgtttc
      601 taaatggctt gcagatggat acgagtggcg gaacatttac tttaaaagga acaacaccat
      661 ctaaccgtta cgtagataat gttgcaacta gtgtaactag tgaagaagtc acgttttctc
      721 ttgatggcac atttatgagt gaagcaggat attataaaca ctgctacgta gaatatagaa
      781 aagacaatca aattttaaca acgcaagata tcattttttt ctcactagga gtgtctgaca
      841 tttcgcaagg ccaagccgat gaatatgttt cgcaattaga agagttgatt cgaaagtaca
      901 acgaaacttt tgatgctttt atggctgaaa tcaaaggtag agtggatagc ttaaatcaac
      961 agattactga tttaactggt caagctaaaa cgctacaaga caagttagat gctctgaaag
     1021 aagaaatttc taagttaggt aacttacaag tgatgtacag taacagcatc gacttcgggg
     1081 actatgatta ttcggagaac ccgaatttaa tgccatatat tacagaacct tgggttggac
     1141 ctttgttagg taatggacat acagttaaag atagtgttaa aagggttata actcatacta
     1201 aaactagaac agctaattcc ggcgacatat tatcgcttgg cttaggtatt ccgtgtacag
     1261 cggaagctaa taataggtat cttataacca ctttacgacc atctacgaca tacacattaa
     1321 gcgttactat gagtgtcggt tcagattgga ctggagagac caatactata ggggttaggc
     1381 ttagatattt aaatgaacaa ggtggtatag aactgcccat taatgcatta ataccagcaa
     1441 atgtagaacg cgataaaatg gtcactcata catttactgg tataacaaaa gataatgtaa
     1501 ctagcataac aaactgttat gttgaaatat tctcacttaa tagtgaatat aaaggaacgg
     1561 ttagtgtcag ctatgacgtt aagttaaaag cacactaccc taacttacta gatggccctt
     1621 actggttagg taaagttcct ttaggcgaga atattgctga ccctacagtt gtctttccac
     1681 acaaaactag tgaatatatg gtttatggta gacgcaatac agaaaactat atcgctgacc
     1741 aaacatatac catatcaatg aaagcaacta aactaacagt tcaatcgttt gccgtgtata
     1801 ttgctgctgg ccgcgttaaa gtaggagaca tgaagcctac tgaggggtta gctaatacat
     1861 gggagttaac ttttactgta actaagcaac atatagatag tggggtaact aattatctgg
     1921 aaatatacca atacccgtca gctacaaaag gagctgttca aattgagtgg ctcaaactag
     1981 aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa atatcgtggt actggtatgc
     2041 gtgattcaaa caatccaaaa gattatgttt gggatctagc accagaatat gtcgaagata
     2101 atttggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat tataccgatg
     2161 ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
     2221 ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
     2281 agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca aacgggaata
     2341 ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt gtggctttaa
     2401 acggaaaaag cagtggtgga aatgattcaa cgatactaac aattccagaa ggctatcggc
     2461 caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg aacagtgctc
     2521 agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt tatggatcgg
     2581 attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat gaaggcagca
     2641 tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga agaacattta
     2701 ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa attcaattac
     2761 tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga gagtacgcag
     2821 gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca ccgcttgaag
     2881 gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat cctaaattga
     2941 atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat ctcaaagcaa
     3001 tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc aaactcatgc
     3061 taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg gatagtttag
     3121 cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac atttcagaaa
     3181 ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag ttttagcgat
     3241 gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt tttcgttccg
     3301 ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag cgcatcttaa
     3361 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
     3421 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
     3481 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
     3541 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
     3601 gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca cgcaacaaat
     3661 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
     3721 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
     3781 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
     3841 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
     3901 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
     3961 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
     4021 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
     4081 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
     4141 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
     4201 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
     4261 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     4321 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     4381 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     4441 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
     4501 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
     4561 cagctaatcc tacactgaca gatggaacga attacgccca cattgatcag tttggagaaa
     4621 tcgaaaatgc aaa
//
LOCUS       DACERL010000123         4617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.128, whole genome shotgun sequence.
ACCESSION   DACERL010000123 DACERL010000000
VERSION     DACERL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.128"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(16..963)
                     /locus_tag="IXF54_002702"
     CDS             complement(16..963)
                     /locus_tag="IXF54_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1405912.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1944)
                     /locus_tag="IXF54_002703"
     CDS             complement(1141..1944)
                     /locus_tag="IXF54_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1405913.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2090..3004
                     /locus_tag="IXF54_002704"
     CDS             2090..3004
                     /locus_tag="IXF54_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1405914.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3052..3522
                     /locus_tag="IXF54_002705"
     CDS             3052..3522
                     /locus_tag="IXF54_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1405915.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3607..4215
                     /locus_tag="IXF54_002706"
     CDS             3607..4215
                     /locus_tag="IXF54_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1405916.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4349..4549
                     /locus_tag="IXF54_002707"
     CDS             4349..4549
                     /locus_tag="IXF54_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1405917.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1478 t
ORIGIN      
        1 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
       61 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
      121 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
      181 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
      241 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
      301 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
      361 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
      421 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
      481 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
      541 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
      601 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
      661 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
      721 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
      781 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
      841 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
      901 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
      961 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     1021 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     1081 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     1141 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     1201 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     1261 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     1321 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     1381 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     1441 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     1501 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     1561 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     1621 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     1681 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     1741 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     1801 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     1861 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     1921 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     1981 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     2041 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     2101 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     2161 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     2221 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     2281 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     2341 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     2401 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     2461 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     2521 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     2581 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     2641 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     2701 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     2761 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
     2821 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
     2881 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
     2941 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
     3001 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
     3061 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
     3121 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
     3181 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
     3241 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
     3301 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
     3361 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
     3421 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
     3481 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
     3541 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
     3601 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
     3661 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
     3721 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
     3781 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
     3841 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
     3901 ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa
     3961 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
     4021 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
     4081 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
     4141 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
     4201 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
     4261 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
     4321 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
     4381 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
     4441 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
     4501 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
     4561 ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag tttttag
//
LOCUS       DACERL010000124         4434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.129, whole genome shotgun sequence.
ACCESSION   DACERL010000124 DACERL010000000
VERSION     DACERL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.129"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(67..1248)
                     /locus_tag="IXF54_002708"
     CDS             complement(67..1248)
                     /locus_tag="IXF54_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1405918.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(1538..1855)
                     /locus_tag="IXF54_002709"
     CDS             complement(1538..1855)
                     /locus_tag="IXF54_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1405919.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            complement(1939..2409)
                     /locus_tag="IXF54_002710"
     CDS             complement(1939..2409)
                     /locus_tag="IXF54_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1405920.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            complement(2426..3358)
                     /locus_tag="IXF54_002711"
     CDS             complement(2426..3358)
                     /locus_tag="IXF54_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1405921.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            4004..4090
                     /locus_tag="IXF54_002712"
     CDS             4004..4090
                     /locus_tag="IXF54_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1405922.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            complement(4210..>4434)
                     /locus_tag="IXF54_002713"
     CDS             complement(4210..>4434)
                     /locus_tag="IXF54_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405923.1"
                     /translation="KDTKVEEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQ
                     AINIQNLTKKETALNYLKSVKEWLDQQQQN"
BASE COUNT         1467 a          691 c          649 g         1627 t
ORIGIN      
        1 attaacttta aggtatcata atttttattg aaatagacat tctattcata aagtgttatt
       61 ttgaattcat ttttgtgttc cttttttttc gttagtatat ctactacaac acaacataaa
      121 tcccaacccc aaaaaattac taagaatggc agaaccacct tgactaatat aggggagtgg
      181 aataccagtt agaggtagta atccgatact tgcacctata ttttcaaaaa catgaaaaaa
      241 aatcattgca acaatgccta taccgatata ggtgaaaaat aagttattgc ttttaaaaca
      301 tgttttatac atttgataaa ttaaataaaa gtataggaat attacaagac aacttcctat
      361 aaagccaaaa ttttctccaa ttacagaaaa tatcatatcg gattctctaa caggaacgtg
      421 aatgtttgat acattaaaac cagtacctaa aagtccgcca gagccaatag caattaaact
      481 atttgctaac tgatagctag atccatttgg atctaaaaat ggattcaacc atgagtctat
      541 tcttttaaac tgatagtcgt gaaatcctat ttttaataag aaatctcttc ctgtatctga
      601 tataaccaaa tataaaaata ttaaggataa aacaacacca atggcgatta ttggggcaat
      661 tatttttata tttatgttag acgtaaacaa aataaaaata taaataacaa tgaagactaa
      721 cattgttcct agatcgttct gaaaaaataa taatattcct attggaagag tagcaataaa
      781 agttttaagt aaataaatcc aatcatcttt cgtattacta gtaatagatt tttgctgata
      841 attgacggtt attttagcta gcataagggt gtatcctatt ttgactaact cagtaggttg
      901 aaacgaaaaa ccagcaatat aaaaccaatt ttttgctcca gttaagactg ctaattgtgg
      961 attatagaat ttcaagagaa gtaataacat tatgatgcat aatccgtaaa atataggagt
     1021 taatttccac aaaattttag gattaaaaaa ataaatagtt attgctgaag tgaacccaat
     1081 actataccac aatacttgtt taattaattc ttttgtgatt tttgaattag tgtaagagtc
     1141 gtgatataaa gcaacatata aagaaagtaa tccaatgata catagcagaa aaactggcaa
     1201 taaaagccca taattcaact gatttttgta gattctatta tttttcattt cgaaaggtcc
     1261 tttttcataa tttataaata aaaataacac aataaagtta tttttactag ttttgagttt
     1321 tagacgaaaa ttacaattag ctactttttt atcttactaa atagttctat ctttttatat
     1381 aaagtataga aaaattaata aaacaattgg aaatatgctg tgaagaaata agtttctatg
     1441 tttattaaag ataataatta aagtattaat tattaatgta ttaattatta atagtaaact
     1501 tagaaaaaat attaataacc aagtggcaat tgaatggcta aaataaaaga atccggactg
     1561 aattaaggtg aaaactaaac ttaaaaaaat tacaaaatga aataatagta ttagtgtttt
     1621 tgtatgattt tttaccataa ttttttacca taattttttc ttctcctaac aaaatattgt
     1681 ctagtgataa atcaaataac ttagcaaact gaattaaatt ttctgtatct ggtattgttc
     1741 gaccattttc ccaattagaa atggtttttt tagatactaa aagcatttca gctaaatctt
     1801 cttgagttag ttgtttttct attctttttt gtttgattac tttacttata gtcatttgat
     1861 cacctaaaca tattttttat tcttagaaag atagagtcta tagaaattga tttcttcttt
     1921 atggaaagtt ggtaattgtt accaaaattt ccaccacttt ttttctgttg ttattgcatt
     1981 gttgttttct ttattgacag aagttaattc ctcttcttca ggttcttccg tataagctaa
     2041 ttgtaactgt tcttgcagtt tttcgatctg ttgatttgcc tgcaaggtga gttgttgttg
     2101 ctggtcgagt aacttttgca tttgttttat ttgagtatct ttttcttgga gctgttcatg
     2161 taaaaaaaca ataatcgagt tttcatcgtt actagcaacg gtttctactt ggttaccagt
     2221 ggtgttacta cctaaaaaat gttgttttaa aagcaaatat cctttgtagc tgacaactaa
     2281 ggttttcact ccgttcctag taacagtttg cacatagttt tctctaaaat cagctgatag
     2341 ccttttccta attgcttgtt ttgaaacatg aaattcttcc gcgagttctt tgatcgtttt
     2401 atctatcata aatactcctt atgcttcaat cgaataagtt ttgtaagttt tctaataaat
     2461 cttgtttaag ctcatttgag attgattgtt tttccgcttc ttctttttcg gtttgttgtt
     2521 gttttttaaa atcagcttca tgagtaccta acggaagatt tagtattcga tctttagcac
     2581 gccatttttc tttttgtgtt aattctccat tgtgttcaat attaaataat tttatacgta
     2641 aatcttcttg cttccctttt gaaaaatcgt cagcatggtt gatttcagct ttccaagtaa
     2701 aacgataacc aataacaggt ttacctcgac ctttaccata ttttttacga atagttaatc
     2761 ctttaaatag tggagttagt tcttctttta ttggtttgag tacaaactga tttatatttg
     2821 tttgtctata gcttttagga atatctaata gctctaaaaa atcttcctta gaaaattcag
     2881 cccatccttg agttcggtat tgttttagca atctaaacat tgttttagca taactgctat
     2941 ttaaatctcg aaattctgtt aaagagaaac gtacccagct ttctagatta ttaagtaaag
     3001 gcaaagcttt ttcgtggact tttatatcta cataagggtt atcgacttct ccatgaattt
     3061 caaaatcagt aaacattgta aatcgttcta ttgttaatcc accattgctt tttcttccga
     3121 aacgtaaatc catcaaatga tcgtatgttc ttgccaaatc ttttataaag acttctcctg
     3181 cagtctgctt atacgcactt aaatctttaa gttggctgaa ataaaagcgt attttttgat
     3241 tacctttatc gcgcattcta gagacaatag aaaaaaacaa gttcatttct attggggtaa
     3301 attttcttaa aggaatggta tttaattctg gttcatactt tacgatttca tttgccattt
     3361 agttgttact ccagtctaaa tgattttatt gacatcattt taggacatat tatgtctaaa
     3421 aacaatacct aaaacagata atttcatgcc tataaacaga taatttcatg cctataaaca
     3481 gataatttca tgcctataaa cagataattt catgcctata aaattttgta taccttgggg
     3541 gagtaaggac acagccgacg ttaaagaggt ttaaaaagag gtttaaaaaa agattataaa
     3601 agagggagac aaaatttccc acctaaaaaa acaattattt tttctttaaa caggttcgcg
     3661 aacttttcta aactgctaat tcaaaacctc gctccctacg gtcgccaaaa aggtgtgatc
     3721 gctacgctct cacattatac ctgcgcagtc gcctccagct cctttgctaa aaccattgtt
     3781 caaaaaggag ggttgaggct ctaagagctt gtctaagccg ttttaagaca gtcttagtat
     3841 aaaaaacttt atctggaatt taaagcgccc tacaggcggt cagggcaagc cctaacaacc
     3901 cggaaataag agatacttaa aattgccaca cagatttaat tttgtagggt ataataactt
     3961 gtcgcctatc cgacagtctt gcgactggaa ggaggtgtat ctgatgtttg aactgtttat
     4021 ttgccccctt cttgtaggag tttgtatctc tgtattcagt tattggttga ataaccgaaa
     4081 caagaagtaa aatcagtaaa ggcgacatca acctaaaaaa agccccagag aatccgacct
     4141 ctctggggct ttcgtgtatc taatgtttga acatttgcat gtttagttta acatgaattt
     4201 caatcaaatt caattttgct gttgctgatc taaccattcc ttgacgcttt ttaaataatt
     4261 taaagctgtt tcttttttag tcagattttg tatgttaatt gcttgttgaa gcaagatttc
     4321 atagcgttca tatgtgactg attgttgtcg ttcttttcgt tcttgttcgc tagttgaaaa
     4381 aggaattgta tctccaaata aatcactaca agcttcttcc actttcgtat cttt
//
LOCUS       DACERL010000125         4434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.130, whole genome shotgun sequence.
ACCESSION   DACERL010000125 DACERL010000000
VERSION     DACERL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.130"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(543..2048)
                     /locus_tag="IXF54_002714"
     CDS             complement(543..2048)
                     /locus_tag="IXF54_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1405924.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXF54_002715"
     CDS             complement(2045..2731)
                     /locus_tag="IXF54_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405925.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXF54_002716"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXF54_002716"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1405926.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DACERL010000126         4274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.131, whole genome shotgun sequence.
ACCESSION   DACERL010000126 DACERL010000000
VERSION     DACERL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.131"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..106
                     /locus_tag="IXF54_002717"
     CDS             <1..106
                     /locus_tag="IXF54_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1405927.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            296..892
                     /locus_tag="IXF54_002718"
     CDS             296..892
                     /locus_tag="IXF54_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1405928.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            1016..2815
                     /locus_tag="IXF54_002719"
     CDS             1016..2815
                     /locus_tag="IXF54_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1405929.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3093..3305)
                     /locus_tag="IXF54_002720"
     CDS             complement(3093..3305)
                     /locus_tag="IXF54_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405930.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4169..>4274)
                     /locus_tag="IXF54_002721"
     CDS             complement(4169..>4274)
                     /locus_tag="IXF54_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1405931.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1270 a          724 c          747 g         1533 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagaatttt
      121 aaaaaaaaca agttattaaa taagtcttta gttggatgtt tttttgagat caatctttta
      181 tacttatgat gaacttacaa aattgtttgt tttctgtcat atcaaaaaat ggcgcagtta
      241 gttgcttttc tagtaaagta tagttaacaa agaacctcta aggagttgat gaataatgaa
      301 tattgctgcc tctcgaaaac tgataaattt catatccatt atcggattag gcctatcaat
      361 cgcgctgacg atttatttta ttaatttagg tgttttcaaa gatttgaatg cgttaagagg
      421 attagtaggt gattccatca ttttaggacc gattattttt gtcttcatcc aaatcctgca
      481 agtagttatt ccaatcattc ctggagggat cagtactgca gctggtgtcc tgatatttgg
      541 cccttatgct ggatttatct ataactatat tggaatctgc attggttcga ttatcatttt
      601 tctgctaggg cgtcgttatg gaaagccatt catactttcc atgattagcg ataagacata
      661 taacaaatat attggctggc tggataatca aaaccgtttt gagaagctgt ttgctctagc
      721 tatttttctt ccagttgctc ctgacgatgc gttatgtttg atggctggat tgacaaacat
      781 gtcagtgaaa cgatatacac taatcatttt gattgctaaa ccactgtcga ttttcttata
      841 tagtatggcc ttgatttatg gaggtcaata tctaagtggt cttcttggat aatatttatt
      901 ttgtaaaatt agtcattgat tgacaatgtt aaatctggga aagggtctgc aaaacacact
      961 gcaccctttc ctttttcatt agatttgcgc tacaatgtga agagcaggtg ataggatgaa
     1021 ttatttgaaa cgtagcttag gtaatgcttg ggacttttta aaaggaacac aagcttattt
     1081 ccgtgatgtc ttgctgatgc atggctttat tttatttatt tgtttaccct tattgagcag
     1141 tacgacccgt tttattttac accgaggggc aattgactat ttgtcttatg acaatattcc
     1201 ttcaattttt tctcaacatc ctggggttct actctcgtta atcgtagttt tgatgacgat
     1261 tttactgtta gtttatttcg aatttacttt tcttttgatg agtgtttttt ttattaaaaa
     1321 gaaagaacca atctctttga agcaactctt acacttgacg atacttcagc tgaagaaagt
     1381 gagacccatt acttttttgt ttttccttgc ttatttttta ttgattctac cgatcagcgg
     1441 attaagtttt aattctgatc tactgtcaaa aataaaaatc cccgctttca ttatggattt
     1501 tatttttgct aatcgttgga tcatcgtcag ttccttttta ctcgtttata ttttccttgg
     1561 ctatattggg attcgactga tttttgcttt accggaaatg attctacggg atcgtccttt
     1621 tcgagcagcc attcgggaaa gctggtcatt gaccaaatcg cgtcttttgg ccatcacggg
     1681 ccaatttatt gtgattggcg gtacgatttt actcctttct tctttaggat atatcgttgt
     1741 tattttggca caaagtatgg ttgaacaatt tttcccagac tatgcattga tcagcgctgt
     1801 gtttgctatg acgcttttac aagggatttt attattcaat atcgtgatgt ctaccgtagg
     1861 gattttttat atcattgttg atttcatgga tgacgaaggc ttcttgcctg agatccctaa
     1921 gtggtttatc ccacaagccc ccaacttgcg tttctctgcg ctgaaaaaca ctggtttgac
     1981 tctttttgca gtttttttcg gtattggcgt ctgtctttac aatatggatt atctgacttc
     2041 agctgttcag acaaaaccag tgactgtctc tcacagaggt gtgagtaccc aaaatggggc
     2101 gcaaaatact ttagaagcac ttgaaaaaac aagcagagac tatcatcctg attatgtaga
     2161 gatggatgtt caggaaacaa aagatggtca tttcgtagtg atgcatgatg caaatcttcg
     2221 tcacttaaca ggggtaaatg gaacaccaca ggatcttaca ttgaaagaat tgacaaatct
     2281 tacagttact gaaaacggag tgcaagcccc tgtttgttct tttgattctt atttggaaaa
     2341 agcacataag ctaaaccaaa aattacttat tgagatcaag acctctaaaa aagattcgga
     2401 tgaccttgtc gaacgatttg tccataaata ccgcaaagtt atcttagaga accatcatat
     2461 tttgcaaagt ttgacgtacc aaaccgtttc agatttaaaa gaagcagaac caaactttta
     2521 tgtgggctac atcttacctt tcaatattgt tgggccccct gtgacgcctg ctgattttct
     2581 gacaatggaa tatagtacga tcaaccgtaa cttcattaat tctgcccacc aagacggaaa
     2641 agaagtatat gtatggacaa tcaacgattc agatgctatg agtcgcatga tgttttatgg
     2701 tgtggatggt atcataaccg atgagatggg actactaaat gaaagtgtga aacaaacaga
     2761 tgaagaaatc acttattcag ataaactgct aaattttgtt ttgggagtcg gctaatcaaa
     2821 aatcgttaat gaaaaaagtc tttatacaac aaaaaacgca taacctgcca ccgcaggtat
     2881 atgcgttttt tgtttgacgc tcataatgtg tgcttaactt tctcttgatt ccttcatcgc
     2941 acgaacaata gctaaaatag caggaattgc agagatcact gcaagaataa ttcccatttt
     3001 ccctgaactt tttctttttg gcatatcttc tcgctccctt ctgtctttat ttatattata
     3061 agggagttct tgcgaattct caaaaatact gtttaagaaa ccaattcagc aggtgcgcta
     3121 atatcacaga attttacgag catgcgatac ttgctttcat tgataagttg atagtctcgt
     3181 tggtcataac tttcaacata cacgacagcc gtgttgatat atgtacgttc aatacagccg
     3241 attagttcat gagatactcc gatcggtttg catatataag atcttcctac ttctgctttt
     3301 accatttcat tccctctttt ctttattttt attttgtttt ttctttattt tataataaca
     3361 aattaccttg tatttgaaaa cgtttttatc cattttttta ttttttattt tgagtatctt
     3421 tctgcttttt agataactat tttttaacac gatcttagac gttgtgacaa aaacgtttgt
     3481 ttatattcct atgttgttcg tgcgtttttt tctttatatt gctaagttat ttataaagag
     3541 gtgagcatat gatttatatt caagcaatag tatttgtaac cttgatgatt gggctactta
     3601 tcggattaaa agaaaatagc taaaccattt aagaaaaaat aaataaggaa aatgataaat
     3661 gctttttaat tgcatcaaaa aacgtacgat gctaaacttg gtataagttc gaaagtttaa
     3721 aggaaacttt caagaactaa attacattac aaaggagatg aattttatga cgtttacaaa
     3781 aacaaatatg caataccgcg tagcttttga tacaacttct aaccgtttta tggcgattga
     3841 tgcacaaaac gaaaataacg ttgcgtttgg tattacaatc gaacaagcag tccgagcatt
     3901 aaacgaatct agataaggag tgaaccattc caaatggcga ctgatgagat ctcggaaaat
     3961 caataaaaga cgtttgtaac ggtaatccgc gacaaacgtc tttttgtatt cattacatat
     4021 atttggaaat tgtctctctt tatctctaga cctaaacaaa aacacaagtt ctaatatcaa
     4081 ccatctcgtt ttttatgaca attttgttag ctgtttcgat gataatattc cgttattatc
     4141 tgtataatat ttaatataat cttaattgtc aaattaagtt agacagatca tcaatactta
     4201 cgtaactcaa aaatacttcc aaaggtgttc gataatccag tgattttcta ggaatattat
     4261 ttcgtttaga tgcg
//
LOCUS       DACERL010000127         4162 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.132, whole genome shotgun sequence.
ACCESSION   DACERL010000127 DACERL010000000
VERSION     DACERL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.132"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(24..1043)
                     /locus_tag="IXF54_002722"
     CDS             complement(24..1043)
                     /locus_tag="IXF54_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1405932.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGNVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYTFIMDYNTGKELARVRADGIY
                     RPDVNQGYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1054..1251)
                     /locus_tag="IXF54_002723"
     CDS             complement(1054..1251)
                     /locus_tag="IXF54_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1405933.1"
                     /translation="MILPDKYYQVIKWTVLTVLPATSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1267..1509)
                     /locus_tag="IXF54_002724"
     CDS             complement(1267..1509)
                     /locus_tag="IXF54_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1405934.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1566..3677)
                     /locus_tag="IXF54_002725"
     CDS             complement(1566..3677)
                     /locus_tag="IXF54_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405935.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLFVNKLESSDF
                     YMGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     NKLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFS
                     NRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSA
                     YEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLT
                     FTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSN
                     NPKYYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAK
                     DAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLKFTSRGGMTV
                     ATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVESNTRKVVAN
                     TAMKKDQWFTVGFSYHSKLSI"
     gene            complement(3701..>4162)
                     /locus_tag="IXF54_002726"
     CDS             complement(3701..>4162)
                     /locus_tag="IXF54_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405936.1"
                     /translation="PGCFFGSWEDGKIARFEQSTVQVYKNFTVRGTKNSTVPTEHYGQ
                     RLLNAYETPEYYFADYGEAVTGDDGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAV
                     THEDVDHFIIETSKPNVLVRWNLVAHRLGYEDVRLKEDTAYDSTVLDQKRF"
BASE COUNT         1159 a          883 c          664 g         1456 t
ORIGIN      
        1 tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca acgtttgtca
       61 tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc ccgcatcatt
      121 acatagactt tcttactagg aaaattacgc atattgaaag atacatgata accaacgttt
      181 ccagaagtat tataaccttg atttacatct ggtctataaa ttccatcagc tcttactcga
      241 gctaattctt tcccagtatt gtagtccata atgaaagtat actcgtattt atagttagca
      301 atgtgccatc cagccacatg caagtttgcg ttttcgattt ctccaaactg atcaatgtgg
      361 gcgtaatttg ttccatctgt tagtgtagga tttgcagcac ctgctcgagt tggatcaatt
      421 actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag
      481 gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg
      541 ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg
      601 tctaatgtta atggaatacc atatttcatt gctgaatctc tagctaattc aacgtatgcc
      661 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacggga
      721 ctgttggcat tagcatacga accagcccca tactgcacat aaccaggttg accgacttga
      781 taaacatttc cgccgtctcc cacaatgtaa gcagtgtaag cactagtcca tgaacgttgc
      841 atatactgtg cttcattgcg tcctgttgct gtttcgttag ctgtttcatg cagtaaaata
      901 tactgattat tcgctacttg agagctacct tcgtttggcc ctaaattaaa ttcattgtta
      961 atcgtatagg caaacccatt aattggcaat aaaaaaagag ccgttaatag gctcatcgca
     1021 gtaatagtaa ttttcttttt catttgtttc ctcctatttt ttcaaattat aagccgacac
     1081 accagtgata acacctaaaa atgttgctgc tgcattgata gtcagaactg ccatatctgt
     1141 tccattccat ccatatgctt tgcctagtgt tgcaactaat acagatgtag ccggaagtac
     1201 cgtaagtacc gtccatttaa tgacttgata atacttatcg ggtaaaatca tttcttctca
     1261 cctcctttac aatttagtca agaaatagcc aatgatcgta atgcctaaac cgatcatgta
     1321 accccacgac catttattat tggctttcat ttctttgata tcttccgcat tattaagcgc
     1381 aatagaatat gcctgatccg ctctatcttt tgcactttcc gctttttcgc gtaatgattc
     1441 gtaattatcc aacttcgttt caatacgcac taaacgttct accacgtctt gtattgcttc
     1501 atctttcaac caactagcct cctttcattg caaaataaaa acgtactcga atgagtacgc
     1561 taaatctata tagataattt actgtgatac gaaaatccaa ctgtaaacca ttgatctttt
     1621 ttcatagcgg tattcgcaac cacctttctc gtattagatt caacatatat ttgcgataca
     1681 gaattacctg tgacagctcc agtccctata gcattgttat caatattaat cattgaatct
     1741 ggcaaatcat taactaaaac ttctccgaat gctatgtcct tagccaactt aaatgatcct
     1801 gtagctactg tcattccacc acgacttgtg aatttaagag atccgccact tactatacta
     1861 gcaggtagca aatatgtttc ttcttttgtt agggccgtaa taatgttttc tatctcatta
     1921 accctactat tcgtactctt tatagcagtc tcattagcgt tcgcttttgt ttgagcatcc
     1981 ttagctgtgg tatctacttc attaattgaa gctgtcaact gcgaattaat ctccgatact
     2041 ttcccatcgg tataattgtt tgctttacct gtgatttcag caaccttgat atctgtagct
     2101 aggttattct caacatagct aggttctaga tcccatacat aatattttgg attgtttgag
     2161 tctcgcatgc cgattcctcg gtatttatat tgctcaatat tcggggttcg agtgtcacct
     2221 ttttcaatct tgagccagtc aattcggcat gcgccggccg tttctttagg cgactgaaaa
     2281 atgcgaaggt ctttaggcaa acccggttca agtttcgttg gtgtgaatgt tagagaccat
     2341 acgtctgtca atccctcaac tggttttaga tctccaaaat taacgttcca ataattatat
     2401 gctacaaagg tttgacttgc gggttttgtt cctttaagcg tgatagtata cgtttgacct
     2461 attataagct cttcttccgt gttaccttta tatatttcgt acgcgctaga tttaattggg
     2521 aacgtcgtac ctttatttgc aatattctta cccaacgatg ttttacctac ccagtaaggg
     2581 tcatctagta agtttggctg atacggagtg gctgttgagc cttcttcgat tttgatgtcg
     2641 tacaatttaa atccaccatt tattttatcc ctgttagaaa aactaacact aatataaaaa
     2701 cgatctaaat ttgtgatttg ataattaacg ttagctgtgc ctttgattgt tatttctttc
     2761 cctacatcat ctattgtaat agttgtatta tttgcttcca ataatatatt ttctcctggt
     2821 gatgtacgat ataccaaacg taatttatta atagctcctg tagttccttc atcaaatcga
     2881 acttttgcac tcagagtata tgttttgcca ctagcaagct gaggtgtatt aatacgcgta
     2941 aacatatcaa tgtcccctgt accatcagac ataaaatgta acattccgtt aacattagtt
     3001 agtgacccat ggtaacccat ataaaaatcg ctagattcca atttatttac aaataagttg
     3061 ggattcccac tataatcata gtcaccgaag tcgatgctgt tactgtacat cacttgtaag
     3121 ttacctaact tagaaatttc ttctttcaga gcatctaact tgtcttgtag tgttttagct
     3181 tgaccagtta aatcagtaat ctgttgattt aagctatcca ctctaccttt aatttcagcc
     3241 ataaaagcat caaaagtttc gttatacttt cgaatcaact cttccaattg cgaaacgtat
     3301 tcatcagctt ggccttgcga aatgtcagac actcctagtg agaaaaaaat gatgtcttgc
     3361 gttgttaaaa tttgattacc ttttctatat tctacgtagc agtgtttata atatcctgct
     3421 tcactcataa atgtgccatc aagagaaaat gtgacttctt cactagttat actagttgca
     3481 acactatcta cataacggtt agatggcgtt gttcctttta aagtaaatgt tccgccactc
     3541 gtatccatct gcaagccatt taaaaatggt ttaacagtca ccgtaattcc tttgtcacct
     3601 tgacgagcca taatagcttt ggtgtagttt aattctttac tgaaatctag agccaaatta
     3661 tataaactgc tagccattta tatacctcct tgtcttcgtt ttaaaaacgt ttttggtcaa
     3721 gcactgtgct atcatatgct gtatcctctt ttaatctaac atcttcatac cctagtcgat
     3781 gtgccactaa attccatcta accaatacgt ttggcttact agtttcaatg atgaaatggt
     3841 caacatcttc atgagtaaca gcacacaaaa ctagttctgt aggtgtcaca tgtgtcatat
     3901 accgacttag atttactgtc tcagcaaaca tgggatcaat atcaacacga actttaccat
     3961 catcacctgt aacggcttct ccataatcag cgaaataata ttctggagtt tcataagcgt
     4021 tcaatagtcg ttgtccataa tgttctgtcg gtacggttga gtttttagta cctctaacag
     4081 taaaattttt atatacttgt accgttgatt gttcaaacct tgcaattttc ccatcttccc
     4141 atgaaccaaa aaaacaacct gg
//
LOCUS       DACERL010000128         4073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.133, whole genome shotgun sequence.
ACCESSION   DACERL010000128 DACERL010000000
VERSION     DACERL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.133"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            591..1280
                     /locus_tag="IXF54_002727"
     CDS             591..1280
                     /locus_tag="IXF54_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405937.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IXF54_002728"
     CDS             1267..2475
                     /locus_tag="IXF54_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1405938.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IXF54_002729"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IXF54_002729"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1405939.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          703 c          812 g         1238 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACERL010000129         3960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.134, whole genome shotgun sequence.
ACCESSION   DACERL010000129 DACERL010000000
VERSION     DACERL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.134"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            161..316
                     /locus_tag="IXF54_002730"
                     /pseudo
     CDS             161..316
                     /locus_tag="IXF54_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            335..1612
                     /locus_tag="IXF54_002731"
     CDS             335..1612
                     /locus_tag="IXF54_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405940.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="IXF54_002732"
     CDS             1622..2278
                     /locus_tag="IXF54_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1405941.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="IXF54_002733"
     CDS             2278..3654
                     /locus_tag="IXF54_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1405942.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1575 a          431 c          690 g         1264 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt tgtaagcgcc ccatagaaca
     3901 gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt ctggaggttt
//
LOCUS       DACERL010000130         3863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.135, whole genome shotgun sequence.
ACCESSION   DACERL010000130 DACERL010000000
VERSION     DACERL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.135"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..957
                     /locus_tag="IXF54_002734"
     CDS             <1..957
                     /locus_tag="IXF54_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405943.1"
                     /translation="LKAAGRKMMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEK
                     KNTRKPYTEIENGVSYIVPKGKRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLT
                     RKSDLRNTLTKVVKEPVRYDRHAMSLLNEVYKNLPKKRSEWHYGDENRTKLSPYTREK
                     LDELTQYVLTKEPGYQEYIQISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNA
                     ILTELKKQDSQIKPLIERMDQMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQ
                     RVVTQRSLRQLNRTINAHEEKYHASKAYEEQQRRIQMEQERQ"
     gene            969..1304
                     /locus_tag="IXF54_002735"
                     /pseudo
     CDS             969..1304
                     /locus_tag="IXF54_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1452..1646
                     /locus_tag="IXF54_002736"
     CDS             1452..1646
                     /locus_tag="IXF54_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405944.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEVESERTFVVTDKEAKAYQPGDL
                     LTLDLTTKKFVDAAENYPFI"
     gene            2055..>3863
                     /locus_tag="IXF54_002737"
     CDS             2055..>3863
                     /locus_tag="IXF54_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405945.1"
                     /translation="MPEEVKGKSSLTLNEEEITGNSRLAQAQRKLIRLRNEYDATFER
                     LAEHQKLTNGQPMNDKRNGSSWFSQREKIENKINQLQEEIQKQENYVEKLTRQSELRK
                     MGYNKYGGLDLTIENIPRIKEEIEKFRNGKSQFSVKTIEKYQKKLVELEQLKEKSEKG
                     IANILPEAQAIIESGRVSQWKRNPTIYFVKGYRKVALELNEQGEFEVSKRYQPKTEEE
                     KRIVSELLENKMDEYGVQKQEPNHYYRVEFNENDGTMRNYQGEIVTQSLLEQIKALDT
                     HYIGKTSYHKFYFEEVKEGEVVNQLRLDVGDGLQLNASIYQKLEELCDQTLETKTEKV
                     IKNEIGSHDKKVETKKPSYQKRSTEEIKQEVKLLSQQALDAVKKYTHSPEDVKELLNF
                     MSRFPERSFRNQLLIEKQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYD
                     KGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRV
                     FDFQLDKKGQAELQAGIQAVANTIGIQIRDMTESEVYQRELGQARGAYVHNEFTGQEE
                     IVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKE"
BASE COUNT         1579 a          573 c          801 g          910 t
ORIGIN      
        1 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagagca actgaatgaa
       61 acaggctact ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca
      121 gttgttgaaa aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat
      181 atcgtaccga aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta
      241 catgagttag aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta
      301 ttgacacgga aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt
      361 tatgatcgac acgcaatgag cttattaaat gaagtatata aaaacttacc taaaaagcgt
      421 tcggaatggc attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa
      481 ctcgatgagt taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag
      541 atttcaaaag cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca
      601 aaggatgcag aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta
      661 acagaactga agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg
      721 caatctattt acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac
      781 aaatatagtt acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg
      841 caacggagtt tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca
      901 agtaaagcat acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag
      961 gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag
     1021 aacgaatcga acaacttccc gattgtttat aaaaagagca tttgtttcag ataagagaaa
     1081 tgttgagaaa cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg
     1141 gtgtgtaatg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa aaatatgagg
     1201 gcaagttagc gccttattat actacttggg aaatgaatga agaggggatc aataacttcc
     1261 cgtgtaaaag cattgaagag tctatttcac tcacggaatg ttaaataaat agagaaataa
     1321 aagagtcctg aacttgattg tccagggctc tttttaagtg aatttttttc cgtcaattca
     1381 ctgagtggtg cgattcaatt ttattatatc aaaagaaagt gagattccaa agagaagggg
     1441 gggaagaaga catgactcag acaacgattg gagttgttgt tgaaaaaagc agagacaatc
     1501 tagtgtttgt gacagaagtc gaatcagaaa gaacatttgt tgtcacagac aaagaagcaa
     1561 aagcgtatca accaggggat ctcttaacac tagatctcac tacaaaaaaa tttgtcgatg
     1621 ctgcagaaaa ttatcctttt atttaagaaa agatggtgaa caaaatggaa caatttcaac
     1681 aagtaaatcc tgaagtacaa gaacaactga agaaatggga atcactttca cgtgaagcac
     1741 aaaatgaaat tatagtgatc ttttctcaaa atcctgttaa tttcagaatt gctttaatga
     1801 aatatgattt aaaggatact gaaaatattt ttacaggatt gggtacgcct atagggaagg
     1861 cttgggaaga acgatacaaa gagttagaaa aagaagttag taccttcaaa gcaacccata
     1921 gagaatggtt agatcaatat gaagatagtg gggttatgta tattccagaa agtttatggg
     1981 agacagctga agagttagga ataatgaaaa actaccccaa agtagttcca actagcgaac
     2041 tcactgcacc agttttgcca gaagaggtaa agggaaaaag tagtctcact ttaaacgaag
     2101 aagaaatcac tggaaatagt cggcttgcac aagcacaaag aaagcttatc cgattaagaa
     2161 atgagtatga cgcgacattt gaaaggctgg ctgaacacca gaaattaaca aatggtcagc
     2221 caatgaatga taaaagaaac ggatctagct ggtttagtca aagagaaaaa atcgaaaata
     2281 aaattaatca attgcaagaa gagattcaaa aacaagaaaa ttatgttgag aaattgacta
     2341 gacaatctga gcttagaaaa atgggatata acaaatatgg tgggctcgat cttacaatcg
     2401 agaatattcc acgaatcaaa gaggagatcg aaaaatttag gaatggaaaa tctcaatttt
     2461 cagtaaaaac aatcgaaaag tatcaaaaaa aattagttga attggaacag ttgaaagaaa
     2521 aatcagagaa aggaatagcc aatattttgc ctgaagcgca ggcaattatt gaaagtggcc
     2581 gtgttagtca gtggaaaaga aatccaacta tatactttgt taaagggtat cgtaaagtgg
     2641 cattagagtt gaatgaacaa ggcgaatttg aagtatcaaa aagataccaa ccaaagactg
     2701 aagaagaaaa aagaattgtg agtgagttac ttgaaaataa gatggacgaa tatggtgttc
     2761 aaaagcaaga acccaatcat tattatcgtg tagaattcaa tgaaaatgat ggaacaatgc
     2821 ggaattatca aggtgaaatc gtgacacaat cactacttga acaaatcaaa gcattagaca
     2881 cacactatat agggaaaaca agctaccaca aattttattt tgaagaagta aaagaaggtg
     2941 aagttgttaa tcagttacga ctagacgttg gagacgggtt acaattgaat gcttctattt
     3001 atcaaaagct agaggagtta tgtgaccaaa cattagaaac gaaaactgaa aaagtaataa
     3061 aaaatgaaat aggtagccac gataaaaagg tagagacaaa gaaaccaagt tatcaaaaac
     3121 ggtcaacaga agagattaag caagaagtca aattgctttc ccaacaggca ctagatgcgg
     3181 tgaaaaaata tacccattct cctgaagacg tcaaagaact cttgaacttt atgtcgcgtt
     3241 ttccagaacg atcttttaga aatcagttat tgatcgaaaa acaatttcca ggagcaacgg
     3301 cgtgtttagg tcgtgctgcg ttgaaaaaag aaggcattta tatcaaaaaa ggggaacaag
     3361 ggaacaagat ctttgtcaga aaaacggtca aaggattcta tgataaagga cggggctttg
     3421 ttcgagaaac agaggctaca ccagaagata aaaaacgaat cgaaagtggc caaatcgaag
     3481 tgatcaaaaa accttactac acgattgaaa aagtgtttga tatcacacaa acacagttga
     3541 aacctgaaga ctatccgaaa atcttcccga accgtgtgtt tgattttcaa ttggataaaa
     3601 aaggacaagc agaactacaa gctggaattc aagcggtagc caacactatt gggatacaaa
     3661 ttcgagatat gacagagagt gaagtctatc aaagagaatt aggacaagca cgtggggcgt
     3721 atgttcataa cgaatttact ggtcaagaag aaattgtcat gaatactaga aatacaccga
     3781 cgcagcattt agctacaagt attcacgaat tagcacatgc cagaatgcac aaattttcag
     3841 aattagacac tgcgaccaaa gaa
//
LOCUS       DACERL010000131         3685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.136, whole genome shotgun sequence.
ACCESSION   DACERL010000131 DACERL010000000
VERSION     DACERL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.136"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..1229)
                     /locus_tag="IXF54_002738"
     CDS             complement(<1..1229)
                     /locus_tag="IXF54_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1405946.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSS"
     gene            complement(1392..1985)
                     /locus_tag="IXF54_002739"
     CDS             complement(1392..1985)
                     /locus_tag="IXF54_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405947.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1986..2318)
                     /locus_tag="IXF54_002740"
     CDS             complement(1986..2318)
                     /locus_tag="IXF54_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405948.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2412..2882)
                     /locus_tag="IXF54_002741"
                     /pseudo
     CDS             complement(2412..2882)
                     /locus_tag="IXF54_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2943..>3685)
                     /locus_tag="IXF54_002742"
     CDS             complement(2943..>3685)
                     /locus_tag="IXF54_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405949.1"
                     /translation="LEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGAAI
                     PSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINSENEKRISDLPKRNQEVQT
                     DDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQFKENDQSFVHQSTAGNVQ
                     MKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPEPIQKVHTGYKPANVAFT
                     APTKINVTRDTKNRNNALKEQLLKDIKP"
BASE COUNT          958 a          751 c          594 g         1382 t
ORIGIN      
        1 tttgaggaag tgaaaccgcg tggtactgtt ccatactctt cataaaaacg atttaaatag
       61 gtcttaaatt ctgctttcat cgttcctttt agctgaggac tcactaactg tacctgatag
      121 accactttgt ctaagtgcgt ttgataggac gctgcttcat tgatcttcaa atctttgact
      181 gatccatcgt catataaact ggaagcttca tcatacgtac gtgtggcaaa tatttctaaa
      241 ggattgatca tcccgtcaga tccgtctaaa cgcaccataa tgccgttata ttcctttaaa
      301 aagggaataa agtcttttcc cttttcaaag ccccaaataa agtctcctgc agctaaatgg
      361 gcttccgcga tcattttaag aacggtcgat ttaccagctc ccatcattcc tagaagcaag
      421 gtattggtag acaaccgcac actattttta tacgtggcat tccaaacaaa cgggccgcct
      481 gtcatggttt ccccatattg tgttccacct ggatctttta gcgtttggtg gtggaacggg
      541 aaacctccgc caatgtttag cgatggtagt gattgccctt tgatatttcc aaaccattca
      601 gcttgttcat cgaacatggt gaacatcgat tgccagtctc ttttcgtttt gtgcagatag
      661 gtcactgcac gataatccat ccctttgaga ttttcgatca gttcctgtgc tcgttcatct
      721 acttcttgaa tcgtgtttcc gtaaacatgt actctcactt tgacttgttt agaaatttcc
      781 ccattagaag tcaattttcg ggcatattcc gctagttcta ggtattcttc cgcgctttta
      841 tttcggtcag tcgcttttcg ttcattatct gaccgatcat ttaattcatc taaggcacga
      901 ttgatttgat caatcacttt ttgcttttca tctgtgctaa catctagtgt tgccactgta
      961 tgaggattgt tcatgagatt ggccaaccaa aaaagcgtag gattttttgt gtagtgataa
     1021 actgtcacac atcgtgaata accatttcca taggtcacat actcttcatc gaaatttaat
     1081 ccaccttgtg gcatcacttg ttttaaaaag tctaaatcat accctttgga ttttaaaatt
     1141 tctatttccc gatttcctag ttgctttaat ggatcaagta ccttttgatc attttgtggt
     1201 gaaaaagctg ttttaattgc ttctaacatg tacagtcacc tcttcttgtt tgattcttta
     1261 cttggttaaa agattttcct tttcccaact tacccaggct taaagtgtga ggaaattttc
     1321 tagtggtctc ttctttcgga acatcttttt ctaaccatat caacggatat ttcattctct
     1381 cttccccatt cctactcatc attcatatta ttcattcgat aaagaatctt gatcaattct
     1441 tttttgtaga gtggttttaa ccctaaaatg ccactagatc gcattaactg acggtttctg
     1501 gtttgcaaat ccttgattgt ctttccttca atgacgaaaa agaagcttaa atcagggcgt
     1561 tcctgttctg ctcgtttcaa ttcctggatt tcctctacta atttcttttc atattttctt
     1621 aatcgctgta gatttcttcc taaggactgt tccatacgat ttttatctaa taactgccgc
     1681 ttttgcacga tttgctccgt tagatccgtt tctgtggtca aactaactaa cttaatcgtt
     1741 ggcacatacg tacgcatgac agtggtcaac tgattcattc gttccagttt ctcttgttca
     1801 tttaatcctt ccacatctgt agaaactaca cgataataac gttgaaaatg atcttcttta
     1861 gtaattaaga ctccctcttc tgtacaggat ttataaggaa ataagtccaa gatagagcgc
     1921 tcttctggtt ttccaaactg caaaatggat tttctgtcaa ctgaattttg acctttgact
     1981 cgcatttatt catcctcctt atacgtacca tgataatttc ggttaggatc taacgcatga
     2041 taatggttct tgtccgtaaa aaaaatagac tcgataatcg caaaaatctg gcgttgacct
     2101 gggttattat agggtcgcca aacaatcact aaagctatcg caatccataa gccataattc
     2161 aagagtgcaa aaaagagtgg taagtggaca agattattga ctattttcgc ccaaaaaaag
     2221 caaccaccaa tcgttaatag atctaaaaag aaaaaggcga aaaacttcca ttccccacta
     2281 aatcttggtg gatttttcat atgaatgtat ttatccataa tgtaaccata ctccttatgt
     2341 tctttatttt tagaaacaaa aaacactgtg atttctcaca gcgtttttct aaatattata
     2401 actctaattc aattttcttc ttttaattca aaaatttttt gttctaggtt tcttttcatt
     2461 tgaatcaact cataattttg tgcgcgtaat tcttcgtttt ccaaatgaac tcgttgcatt
     2521 tcatcgttta atcgatttaa attctccaat gattcttgta tcgtttcttg cctaaaatta
     2581 agtgagccaa gttgacgatc tttttgttgt ctttgctctt tcaattctga cgatatttgc
     2641 tcaacagaat gaaatagctc cgttaatttt actgtgaatg tgggcatagc atctttcaaa
     2701 taagtgaatc cttctgaaag gtgtacaata atgctttcgg tatgtgtttt ttgaccatct
     2761 aaaatagact gatgatcttt ttgtaaactc tccgtatctt taaatccttt aatagtcgcc
     2821 acaaatatcg ttaaaaatgc tcccagtgca gttataaacg tagctattaa aatatcctcc
     2881 acaattttct cctttccatt ttaaacttgt tttataaaga aaaaggccct taaataggcc
     2941 ttttatggtt tgatatcctt caatagttgt tctttcaatg cattgttcct gtttttggta
     3001 tctcttgtca catttatttt tgtcggtgca gtaaatgcca catttgctgg tttgtaacct
     3061 gtgtgaactt tttgaatagg ttctggtgca gcttctcttg tcacaacatt ttgaacggac
     3121 gcattccctg tctccccact ttcagataaa ttgtaagaaa cagttgtcag atttccattt
     3181 tcaggcaaac gacttggttt catttgaaca ttacctgcag ttgattggtg aacaaagctt
     3241 tgatcattct ccttgaattg attcaaacga ctatctttca ttggtaaatt ttgtgcctta
     3301 ggtaaaccat ttttcgttga tttttcagtt ccaacttcga ccccattatc atttggcaga
     3361 tcgatggaat catcatccgt ttgtacttct tgatttcgtt ttggtaaatc tgaaatgcgc
     3421 ttttcatttt cagaattaat gttagattta taacgatatt gttctggatc tgtttttggt
     3481 aattgaccat tttgtccttc ttctgaattt tcgttagaat gcaactcatc ttggtgatct
     3541 ttggctgaag gaatcgctgc accttcttga ttttcttttt cggttgttgt attagctttt
     3601 actgctgcat tgacttgtcc ttcaccatac gcatctttta gtccttcttt gacttctgaa
     3661 gctttttctt tttgtttttc tagtg
//
LOCUS       DACERL010000132         3630 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.137, whole genome shotgun sequence.
ACCESSION   DACERL010000132 DACERL010000000
VERSION     DACERL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.137"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(45..2231)
                     /locus_tag="IXF54_002743"
     CDS             complement(45..2231)
                     /locus_tag="IXF54_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1405950.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(2231..2440)
                     /gene="copZ"
                     /locus_tag="IXF54_002744"
     CDS             complement(2231..2440)
                     /gene="copZ"
                     /locus_tag="IXF54_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1405951.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(2453..2893)
                     /locus_tag="IXF54_002745"
     CDS             complement(2453..2893)
                     /locus_tag="IXF54_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1405952.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(2967..3440)
                     /locus_tag="IXF54_002746"
     CDS             complement(2967..3440)
                     /locus_tag="IXF54_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1405953.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT          934 a          833 c          625 g         1238 t
ORIGIN      
        1 ttttctccat atcgttccca ttcattttta ctccatccct tctcttactt tttcatgtgc
       61 cgatttaagc tcagggagtt caacaataca ctgacggaac taaacgccat cgctcctcct
      121 gcaatgattg gattcaaaaa gcctagtgcg gcaaaaggaa taccaatcgt attatagaca
      181 aaagcccaga aaagattttg tttgattttt ctcaatgtca ccctagacaa ctcgattgtt
      241 tgtgcaatgg atgccaaact gctactcatc agcgtcacat cggctgtttc catcgcgata
      301 tccgttccac ttcccattgc cataccaaca tcagctaatg ctaaagccgg tgcatcattg
      361 attccatcgc ctgccatacc gacttttttg ccatctttct gaagtttttc tacgtaggct
      421 gctttatcct ctggaagtac ttcagcaaag atatgctttg gatcaatacc aacttgttta
      481 ccaatcgttt cggctgccag tttgttgtct cctgtcagca taaaaacatc taatccttta
      541 ttctggagct gcttgattgc ttgtttggct tctagtttga tttgatcagc cacggcaatc
      601 aacccgatca cttgcttttc atcagctaaa aacatgactg tttttccctg ctgttctaat
      661 tctaacgcgt gttcttggta ttcatcaaat gataggttca attcaattaa tcgtttacgt
      721 gtacccgcaa aatagcgtac tccgttaatc gtaccgctga ttccagcacc tgggtgagct
      781 gtaaaatcaa tcatatcgta tgttgctgct tcttgcagtt ttccgtattc cacgattgct
      841 ttacctagtg gatgttcaga agcttgttct aaagtataaa aaatagatag gacttcttta
      901 gaaccgacta gatctgtaac tttgggcttt ccttctgtaa tcgtcccagt tttatccaaa
      961 acaatgctgt ctaagtgagc agctgcttct aatgcttctc cgcctttaat taaaattcca
     1021 tttcgtgcac caacacccgt tcctaccata atagctgttg gagtcgctaa acctaatgca
     1081 catggacacg caatgactaa tacagagaca ctatggatca atgcgagttg ccaatcacca
     1141 gtgatcaaac ctgtagctat gagcgtgata aacgcgatga ctaatacgat cggaacaaaa
     1201 atccctgaga tccgatctgc gattttttgg attggggctt tgctcccttg agcatcttcc
     1261 accatttgaa tgatttgcgc taatactgtc tgcttgccga cttgtgtgac ttccatgtgt
     1321 agcagaccat tcgtattgat tgtcccgcca aaaagcgtgt catccggatt cttttctaca
     1381 ggaagatttt caccagtaag catactttca tctatcgcac tgcttcctga aataattcga
     1441 ccatctgctg gaatctgctc accaggacgg atgactagtt gatcaccaac gactacttct
     1501 tcaatcgcta acgtttcttc tttcccgttt cttatcactt gggcggtttt tgtttgaaga
     1561 gacatcaatt gtttgattgc attacctgtt ttcgtttttg ctgtatgttc caaatatttg
     1621 ccgagcaaaa tcagcgtgat gatcatactg ctgctttcaa aataaagatc ttgggggtgt
     1681 ccacggaaaa atccgttgta aatgctcaat gcaaaggcag ccgatgttcc aattgctacc
     1741 aatacatcca tatttggtgc tttcgacttg attgcatggt acgcaccttt ataaaaacga
     1801 gcaccgacat aaaattggac aggagcagac agcactaact ggacgattgg aaaatgaaaa
     1861 aagtgaacaa tcgccgcatg gcttcctaac atcatagcaa tcattgacaa catcaacggc
     1921 aaagtcaaaa ccgtactcaa tataagatcg aatagcattt ttttcaaata cgcttgcttt
     1981 tcttctgcaa ttttttgttt gtgggcttca tcgtacaaaa ttgcaccata gccgatgttt
     2041 tctacgcttt ggattaattt ttcagtggtc gtcccttcat atttgacact tgctttttca
     2101 gtggccaagt tgactgtggc attgaccaca ccaggttgtc cgttcagctc tttttcgatc
     2161 ctagcagaac aatttgcaca agtcatacct gtaatgacga acgtatccat tttggtttct
     2221 gctgttgcca tcaaatcacc tctgctttat aacccaatcc attgattgct tgacaaatct
     2281 tttcggattg aacttttgtt tcatcaaact tgaccgtacc gtttgctttt ttcaaattta
     2341 ctttcacttt ttgcacgccg tcaagtgcgc ttactgtttc ttctactctt gctgcacaat
     2401 gattgcagct cataccttcg attgaaaatt gttgtttcat tttttcttcc tcctattgat
     2461 gttgtttaca ttcacattga cctgggatac agttacattc gatcgtatct actggttgct
     2521 tttgttccag ttgtttcaaa atctcattga tatcttcttg agtcaaagtg gcttcttcta
     2581 ctagatccgc aatcgtctct ccaatccttt ttgcacagat atgggaaaaa agattttccg
     2641 tcgcactgcg aactgtttcc atctccgaga cagctggatg ataaatgaat ttttttcctt
     2701 cttgttctgt ccagatcact tcttttttta ccaaacgacc cagcaaagtt ttgatcgttg
     2761 ctggtttcca atccatcgaa tcagctaaga tctgcgtgat ttcttgggct gtcgtctgtc
     2821 caagtgtcca aataacacgc atgatctccc actctgaatc actaatcttt actggcgttt
     2881 tgactttttc catttatttc acacctttcg tttacaaatg taatcattaa cttatgaata
     2941 cagattacac ttgtaaacaa aaactgtcaa ttatttttgc tctctatttt caatttagtt
     3001 aaaaattctt gtttctctcg tcttgtttta aggatgatca gtttatttct tgcattagct
     3061 acttggatca gctgctgtat ttttggttca ttgtttcctt caaaatgcca gataaaagat
     3121 aaaaattcta agtatttcct gtttagtttc tccgaaaatg attcaggcat atctggccga
     3181 gattttttgt ttatctttct ttttagagaa cgccaaacta ctcggtagat ggcgagataa
     3241 cgcggcactt taagccaaat gatcttatct gcttctttaa tcctttcctc taacgtagaa
     3301 gtataatttc cttcaacgat ccatgacgga ttagatacca taaacgattg ctgttcttgc
     3361 aaaaaccact tttttgcctg aatgcggaaa gccggtgatt tctttgatct tccttgcgag
     3421 ggacgatttt cccgacccac tttgtccaat cacgatgatc ttcattcttc tccctcactt
     3481 ctctttggaa tttggttcga tcataccact tataacgccg caaaaaaaca agggactgtg
     3541 acaaaagtcg tgtcaaagtc ccttgtttcg aataaatggt gttcaatcag ctgaccttca
     3601 gcattagctc aaaaaacggc aaaatatgag
//
LOCUS       DACERL010000133         3340 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.139, whole genome shotgun sequence.
ACCESSION   DACERL010000133 DACERL010000000
VERSION     DACERL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.139"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(87..722)
                     /locus_tag="IXF54_002747"
     CDS             complement(87..722)
                     /locus_tag="IXF54_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1405954.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(988..2040)
                     /locus_tag="IXF54_002748"
     CDS             complement(988..2040)
                     /locus_tag="IXF54_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405955.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(2053..2991)
                     /locus_tag="IXF54_002749"
     CDS             complement(2053..2991)
                     /locus_tag="IXF54_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1405956.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1138 a          707 c          514 g          981 t
ORIGIN      
        1 gttctatgat atttggtgtc cttactcgcc ttgagtaaca acaccaaata tcatagaacc
       61 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
      121 atcggcaaat agttaaagaa aaaacttaat cgttgaaaga agcccaattg cttaaagaag
      181 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
      241 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
      301 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
      361 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taagccagta
      421 aaaatacaat cacctatccc atataaacca actgttgcaa caacaagtat tgcaagtgtt
      481 tttgatgttc caacaaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
      541 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
      601 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
      661 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
      721 attttcttcc cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
      781 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
      841 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
      901 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
      961 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     1021 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     1081 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     1141 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     1201 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     1261 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     1321 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     1381 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     1441 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     1501 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     1561 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     1621 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     1681 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     1741 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     1801 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     1861 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     1921 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     1981 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     2041 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     2101 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     2161 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     2221 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     2281 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     2341 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     2401 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     2461 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     2521 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     2581 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     2641 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     2701 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     2761 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     2821 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgtt ctttgatttg
     2881 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     2941 caacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     3001 ctcctttttc tttctattat actcatttga gaatcttttc acaacctcgt tttgttattc
     3061 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     3121 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     3181 aacattcata gaaaaaatct cactcctctg attataaaag agggggcatt aacctcgtgt
     3241 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     3301 tagtacgatg ttgcgcaaca cagacggatg gtattgctta
//
LOCUS       DACERL010000134         3274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.140, whole genome shotgun sequence.
ACCESSION   DACERL010000134 DACERL010000000
VERSION     DACERL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.140"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            177..473
                     /locus_tag="IXF54_002750"
     CDS             177..473
                     /locus_tag="IXF54_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405957.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="IXF54_002751"
     CDS             480..2432
                     /locus_tag="IXF54_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1405958.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IXF54_002752"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IXF54_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1405959.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1156 a          538 c          566 g         1014 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca
     3121 taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc
     3181 attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca
     3241 ttttgtcatt ttgtactcca aaactgaact actt
//
LOCUS       DACERL010000135         3203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.142, whole genome shotgun sequence.
ACCESSION   DACERL010000135 DACERL010000000
VERSION     DACERL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.142"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1646..2164)
                     /locus_tag="IXF54_002753"
     CDS             complement(1646..2164)
                     /locus_tag="IXF54_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1405960.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1079 a          567 c          453 g         1104 t
ORIGIN      
        1 ataaagaaaa aattcaagca acaattcaaa aaaatcttaa ctgctgagat tgatgtagca
       61 atatctcaat catcaacaaa gtacgatcct gcctttcaaa agcttattga taaatgttta
      121 ttcattataa aaataaaaga aaaatttatg tcagcttttt tcatcaaagt ggtaatgata
      181 attcgtctat taattgtatt ttttaggaaa tatggtataa ttgttttaac gaaaaggtaa
      241 ttcatgatac accaaaaacc actagctggc gggctagtgg ttttctttta cgttctattc
      301 agattaacgg gtaggcttac tgtctttttt ggcaataatt ttgcaatcaa gccatttaca
      361 gatgtaatgc gcggatactt ctgctaagat tgcaaaaaac atatcgaaaa ggtaattcat
      421 aatacaacct cctccccttg ccataatagg ccggggtagc agacagcata aatattatac
      481 cataactgaa aatttcattt tagtacattt caatatattg atgaaccagc acaataaaac
      541 atgccatgac taacagaaaa aaacttatta aaccagatat aacttgtaaa catccaacta
      601 tatagaaaac gtaaattttc tatatagttg gattaagata acgactattt attttatcat
      661 atttttacta taccctatac ttttacttgg tatatttatg aataatcgca aatttacccc
      721 tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt tcttttatta
      781 aatacaccta caacttataa attgatggta tttagataac acgaaagaat aaaaataaca
      841 gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa
      901 cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa tcgttactgg
      961 gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct actatttgat
     1021 ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg tatagaattt
     1081 atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac cctttgatgg
     1141 ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta acaaaggcta
     1201 tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt cttcctaacc
     1261 caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt gttgagattc
     1321 catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca aaaataaaaa
     1381 aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc ataatctcat
     1441 tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca atctaacgat
     1501 gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa accggcagtt
     1561 agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt ctcctttcca
     1621 caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt cttatctttg
     1681 ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat acttcccata
     1741 cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat agatcttcat
     1801 cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga atttcctcta
     1861 attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac tctcctaact
     1921 gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt tcatcatttt
     1981 cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca gcaggtgttc
     2041 tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca aattctaaaa
     2101 tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt ttatatgttc
     2161 tcattatatt acctccataa attcctaatt gtctccttta attcatctat aatttcatcc
     2221 acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc taactcatat
     2281 gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc gtcagcaagg
     2341 agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg catatcattt
     2401 tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa ctaccacttt
     2461 aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc cattgttaaa
     2521 cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc gctctttctt
     2581 cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca cgttccttac
     2641 ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct
     2701 aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc aagatttacc
     2761 tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca tttataatac
     2821 tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac agatacactt
     2881 gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta atcttccgaa
     2941 atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa
     3001 aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg ttgacacggt
     3061 gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc taaaaagtct
     3121 atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc aacttatatg
     3181 ttttagtgaa ctacccccac tta
//
LOCUS       DACERL010000136         3036 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.143, whole genome shotgun sequence.
ACCESSION   DACERL010000136 DACERL010000000
VERSION     DACERL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.143"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            714..2630
                     /gene="ltrA"
                     /locus_tag="IXF54_002754"
     CDS             714..2630
                     /gene="ltrA"
                     /locus_tag="IXF54_002754"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1405961.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSTLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            2747..3036
                     /locus_tag="IXF54_002755"
                     /pseudo
     CDS             2747..3036
                     /locus_tag="IXF54_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT         1160 a          491 c          631 g          754 t
ORIGIN      
        1 tgtgacctgt ttagcctaag gataaaagag gctagttcca acgaacattg gaataacttt
       61 actatcgaga aatcaaatga cggggtaacg ccctgaaggg tttccactga tactgcgaca
      121 tgcgttagta ggatgtgcat ccaaacgcgt atgaagctcg ctgaagtcgg cagaagggta
      181 ccgtaaggaa agacacgata cgtatctacc acgcaagtgc tctagaggta gggtatgtac
      241 ctgatatgtc gggtgtccta aaaagttcaa taaggtgaga atgatttgat tatctgacaa
      301 acttgcgaat ggaatgtctg aaacgaataa ctcatagaaa tgtgaggggg aagggggata
      361 cgtcctcttt ccattgactc gaggttgagt aaaaatgtgt ttctatgaaa aaccttgcat
      421 ggttaaaggc tatggagtag tcaacaggca aaagcaagca cctaagttga aatctatgga
      481 tagatagtaa ggaacagggg aagcatctag cacggaggga gcataccgaa gatgtgtttc
      541 taaggaaaaa ccgaaatcgg ttataactta ggtgaataga tgtgaaagta gagccacgac
      601 cagtgaaaat ttcgtaatga aattggagga acaggctcaa gtcgttattt ataatataag
      661 aaacctattt aaaatcatgg attctcgtaa gactaaaaag gtcacgaacc agaatggagc
      721 gtgataaaac catgactaac acaaaaggaa ttagcttaaa gaacaaacaa ctcagacacg
      781 ccgaatacta cgacatgact gagactttcg atgaattgca cgcaatgagt gttgcaaaat
      841 caaattttaa aaatctcatg aaattcatta ttgatgacaa taatatcttt ctagcgtata
      901 gaaatatcaa aaagaataaa ggaagtgcaa cggccggata cgatggtgtc actataaaag
      961 acattgaaaa attggcatgt ggtgactttt taactattat taaaaggcga tttaaggatt
     1021 acagaccaag aaaagttaaa agagtagaga tcccgaaacc aaacggaaaa cttcgacctt
     1081 taggaattcc ttcaatatgg gacaggatag tccagcaatg tattctacaa attttagaac
     1141 caatatgcga agcaaaattt aataagcata gctatggatt cagaccaaat tgttctacag
     1201 aaaacgccat agcagattgt cttgctagaa tcaatcaaag taagatgaat tatgttgtag
     1261 atattgatat aaaaggattc tttgacgaag taaaccatac caaacttatg cgtcaaatat
     1321 ggacaatggg gattcgagat aaacaactcc ttgtaattat tcgtaagatg ttaaaggctc
     1381 cgattgttat gcgaaatggc aatgttgttt ttccaaccaa gggtactcca caaggaggga
     1441 ttttaagtac actgcttgcg aatatatgcc tgaatgaatt tgattggtgg atagctaatc
     1501 aatgggagga acgaactgtc aaagagctga aacaccacta tcgaaaaaac ggagcatggg
     1561 ataaaggaaa tatctattcg aaattaagaa gccacacaag tttaaaagag atgtatctgg
     1621 tacgctatgc agatgatttt aaaattttct gccgaacagg agaagaagcc caaagggctt
     1681 tttatgccac acaaaaatgg ctcagagaaa gattaaagct cccaatttca atggagaaat
     1741 ctaaagttac gaacctaaat aaagctgaaa gtgaattctt gggtttttca ataagactcc
     1801 acaaaaaggg aaaaaagaaa gtggctttca cgtacgtttg tcgcaaagca gaagaaaaca
     1861 ttaaaaataa actaaaacaa cagattaaaa aaattcaata cgctggagca aaaaaaaata
     1921 ggcaccgagc aataggtgac tacaacatga tggtcatcgg tatccacaat tactatcgga
     1981 tagcttcaca ttgtagtgtc aatttcactg agatttatag aagtgttagg acagtaatag
     2041 aagcaagact gaaagctaga ggaatgacga aaaatggaat ctatggacaa gatcatgaag
     2101 gataccgtcc gtatctaaag tctaagcaaa tgagatatat tgaaggtttt ccgatacttc
     2161 caattggcta tattaaaaaa agaactccac tcaacaagcg gagaaatcta aataaatata
     2221 ccaaggaagg gagaaagctc attcatcaag agcaacaagc tatagaaagc tggaaaatca
     2281 agtatcttgt aaaaggtttt agtaacaata agaaatacac ggtggaattt tctgataaca
     2341 aaatatctaa gtttatttcc caaaaaggga aatgcgctat cacaggaaac gaacttttga
     2401 tggaagaaat tgaatgccat cacattaacc catatcattt gtccaaagac gatagttaca
     2461 agaaccttgt cattatctct agagaaatcc atagattaat ccatgcaact agtgaagaaa
     2521 ctattctgaa actgcttcgg ttactgaaac taaatcacta tcaaattaga aaactaaaca
     2581 cgtatcgaaa aaaagcagct aattttccaa tatcaatcaa aatgaattag ataggaaaca
     2641 aattatattg taattaacga tggaacgccg tgtgcggtga aagtcgcacg cacggtgtgg
     2701 accgggggaa aagacggaga taatatcaaa ctcttaccta tcggaattgc caccaatgga
     2761 taatatggaa ggaatcatga cggtcacagc tggccgaaac atgttgtggg atctgtttat
     2821 tcaatcttat cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga
     2881 aaattgtcag acacatattt atattatgtc gacagatgat aataccatcg aagaattttc
     2941 taaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat
     3001 gaatactcat atcaatcgca atgttgattc agatcg
//
LOCUS       DACERL010000137         3016 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.145, whole genome shotgun sequence.
ACCESSION   DACERL010000137 DACERL010000000
VERSION     DACERL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.145"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(106..1053)
                     /locus_tag="IXF54_002756"
     CDS             complement(106..1053)
                     /locus_tag="IXF54_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1405962.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IXF54_002757"
     CDS             1299..1763
                     /locus_tag="IXF54_002757"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1405963.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IXF54_002758"
     CDS             complement(1825..2868)
                     /locus_tag="IXF54_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1405964.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          598 c          577 g          845 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttct
//
LOCUS       DACERL010000138         3014 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.146, whole genome shotgun sequence.
ACCESSION   DACERL010000138 DACERL010000000
VERSION     DACERL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.146"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            54..1514
                     /locus_tag="IXF54_002759"
     CDS             54..1514
                     /locus_tag="IXF54_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1405965.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRKKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYILKDESKVERVEN
                     LHFETQEYKHHPMDYQPLTEKGRTYLKEQRKFPEWLIEYGDQQGLILEMKPRSERKNY
                     IVKDDRLDYAIVFPWKDRHTDQVLGATYQGTIIDYERFGDRGTYKHIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYVALNRETVKDTWLISMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHIFWDKVHRLGVSNPFTGKAVYFEPDLPNDWQVPRKYQTI
                     YEEVGKEMNVAPEAIMAIHKIENNLTENNQIVLFGEIHGVFAKKLASKEKVQIIDVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEEIQISLKAKHYFEGYKNHDHEFVSE
                     VKKDWNDQWKHEIQQQEIRKQKRAMLFQQSRQQERE"
     gene            1525..>3014
                     /locus_tag="IXF54_002760"
     CDS             1525..>3014
                     /locus_tag="IXF54_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1405966.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGAEQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNDKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITA"
BASE COUNT         1074 a          511 c          664 g          765 t
ORIGIN      
        1 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
       61 agaaaaaaat gagtcttatt gatcgatgca agcaaatgga tattgtagat tttgctcgaa
      121 acaatggttt agcggtagtc aataaaggac gagactatcg tttagaagac catgattctt
      181 ttgttttcga tcgaaaaaaa cagcgatttt attggaacag tcaagggatt ggtggagata
      241 ttattgaatt gactcaattg ttttttattg acgattctat tcaaaaccca aagcaacgtt
      301 ttaaagcggc gctagattat attttaaagg acgaaagcaa ggtagaacgg gtggaaaatc
      361 ttcattttga aacacaagaa tacaagcacc atccaatgga ttatcaacca ctcacagaaa
      421 aaggacgaac ttacttaaaa gaacaacgaa aatttcctga atggttgatt gaatatggag
      481 atcaacaagg gttgatcttg gaaatgaagc cacgaagtga acgaaaaaac tatattgtga
      541 aagatgatcg tttagactac gcgattgttt ttccttggaa agaccgacat acagatcaag
      601 tattaggagc aacttatcaa ggaacgatta ttgattacga acgttttggt gatcggggaa
      661 cgtacaaaca tatcgatatc aatcctactc ctaatcacgg atttaatgtc cttcttggtt
      721 ctcctaaaaa tttaaaattt tttgagagtc ctattgattt attgagttac gttgctttaa
      781 atcgtgagac agtcaaagat acatggctca tttctatgga cggactgaag gatgcagtag
      841 taggacacta tgttcgagaa gcggtaacgg aattagcgcc gaaagaggaa tttccagaat
      901 cgatcgagat ttgtgtggat aatgatcgag ctggacacat attttgggat aaggtacacc
      961 gactgggagt gtcgaatcct tttacaggaa aagcggtgta ttttgaacct gatcttccca
     1021 atgattggca agtaccgaga aaataccaaa ctatctacga agaagtaggg aaagaaatga
     1081 atgttgcacc agaagcgatt atggctattc ataaaatcga aaacaatcta acggagaata
     1141 accagattgt attattcggt gaaattcatg gagtatttgc caaaaagctt gcgtcaaaag
     1201 agaaagttca gatcattgat gtgaaagaga aatgtataga agccgctcag cagttaaaat
     1261 cgtgtgaaaa atctgatgga acgtacgatt ttgatcgctt ttatcgtgga aaagggaaaa
     1321 ttaatgaaga aattcaaatt tcattgaagg ccaaacatta ttttgaaggt tataaaaatc
     1381 atgatcatga atttgtttct gaagtgaaga aggattggaa tgatcaatgg aaacatgaga
     1441 tccagcaaca agaaattaga aagcaaaaac gtgccatgtt gtttcaacaa agcagacaac
     1501 aagaaaggga gtgaaagaag cacgatgaaa actgtcatat tagcagaaaa gccgtcacaa
     1561 gcgaaggctt atgccgatag cttctctaaa gccactcgaa aagacgggta ttttgaaatc
     1621 caagatcgat tgtttcctgg tgaaactgtg atcacgtatg gttttggtca tttagtcgaa
     1681 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga acatttgcct
     1741 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaaggtg
     1801 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
     1861 ggggaactga tcgcttggac gatcattcaa caagcaggcg cagagcaggg aaagaccttt
     1921 aaaagacttt ggatcaactc cttagaaaaa gaagctattt atcaaggatt tcagcagtta
     1981 agagacgcag gagaaaccta tccgaaattt gaagaagcac aagcacgcca gatcgctgat
     2041 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaaaggcata
     2101 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcaattg
     2161 caggaaaaga ttagaaattt caagaaagaa ccttattttg aaggaaaagc cgaagtaatc
     2221 gctcaaaatg gcgcatttga tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa
     2281 gcatttgaag attatttaaa agaaaagggg gttcaattgg gaaaacagcc aggcatgatt
     2341 cgccaagttg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatctagc
     2401 cttcagtcta aaatgaatca actcatgaaa gctagtgcca aagacacttt ggaagccatg
     2461 caaggcttgt atgaagggaa atatctaagc tatccgcgaa cggatacccc ttatattacg
     2521 gaaggggaat atgcgtatct gttggatcac ttagacgaat acaagcattt tttgagggct
     2581 gaagaaattc caacccctat tcatacgccg aatagtcggt atgtgaacga taagaaagtc
     2641 caggaacatt acgcaatcat tcctacaaaa acagttatga cagcagcggc ttttgaacaa
     2701 ctaagtccac tgcagcaggc tatttatgaa caagtgttaa aaacaaccgt ggcgatgttt
     2761 gcggaaaaat atacctatga agaaacaaca atcttgacgc aagttcagca gcttcagtta
     2821 aaggcaatcg gaaaagtacc gatagatcta ggttggaaga agttatttgg caaagagagc
     2881 gacggaaaag agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg
     2941 acggtagatt tacaagtcct tgagaaagaa accaagccac cacaacctta taccgaaggt
     3001 acgttgatca ctgc
//
LOCUS       DACERL010000139         2963 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.147, whole genome shotgun sequence.
ACCESSION   DACERL010000139 DACERL010000000
VERSION     DACERL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.147"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            747..2624
                     /gene="ltrA"
                     /locus_tag="IXF54_002761"
     CDS             747..2624
                     /gene="ltrA"
                     /locus_tag="IXF54_002761"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1405967.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2755..2963
                     /locus_tag="IXF54_002762"
                     /pseudo
     CDS             2755..2963
                     /locus_tag="IXF54_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         1124 a          464 c          643 g          732 t
ORIGIN      
        1 agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa
       61 aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct
      121 tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg
      181 gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctt
      241 tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat
      301 gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg
      361 ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt
      421 gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat
      481 ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg
      541 catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg
      601 gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat
      661 tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc
      721 acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt
      781 tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt
      841 tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc
      901 tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa
      961 cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat
     1021 tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat
     1081 taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag
     1141 tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata
     1201 gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat
     1261 atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga
     1321 gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt
     1381 taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac
     1441 aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga
     1501 tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg
     1561 gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa
     1621 tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga
     1681 aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg
     1741 aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga
     1801 tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac
     1861 aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata
     1921 gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg attcataatt
     1981 acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac
     2041 caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt
     2101 acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa
     2161 tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata
     2221 aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga
     2281 agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg
     2341 acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc
     2401 ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata
     2461 gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa
     2521 gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac
     2581 tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac
     2641 gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta
     2701 cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt
     2761 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
     2821 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
     2881 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
     2941 atgcgtatgg atgaatctat ttt
//
LOCUS       DACERL010000140         2944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.148, whole genome shotgun sequence.
ACCESSION   DACERL010000140 DACERL010000000
VERSION     DACERL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.148"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            6..977
                     /locus_tag="IXF54_002763"
     CDS             6..977
                     /locus_tag="IXF54_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ1405968.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IXF54_002764"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IXF54_002764"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ1405969.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IXF54_002765"
     CDS             2004..2612
                     /locus_tag="IXF54_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ1405970.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          779 a          625 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcccg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACERL010000141         2940 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.149, whole genome shotgun sequence.
ACCESSION   DACERL010000141 DACERL010000000
VERSION     DACERL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.149"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(47..1237)
                     /locus_tag="IXF54_002766"
     CDS             complement(47..1237)
                     /locus_tag="IXF54_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1405971.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            1350..1472
                     /locus_tag="IXF54_002767"
                     /pseudo
     CDS             1350..1472
                     /locus_tag="IXF54_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            complement(1636..2937)
                     /locus_tag="IXF54_002768"
     CDS             complement(1636..2937)
                     /locus_tag="IXF54_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1405972.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT          906 a          523 c          533 g          978 t
ORIGIN      
        1 ggagcgtcaa taattttgtg taaaagtata tccttggtaa agcagattag ctcttgaacc
       61 aatcttctct ggttgtttgt ccaaaacctc tatgtacgtg attcatgttt ttctcgttgt
      121 aacgattgaa ctgaatcaca aggtatttct cttcagattt ttccgttgga aattgttctt
      181 tctttttgaa acttcgtttt aattgtttat tgtttccttc aatcagattt gttgaataaa
      241 tcgtatgcct aacactttct ggataatcat agaatgtaaa cagatagtca ttgttgctga
      301 gacttttcac cattgatgga tatttgtgtt tccacctatc gatgaatttc tttagagaag
      361 ttaaagcttc ttcttttgat ttggaccgat atacctcttt aaattcattg gccacttctt
      421 ttctgtcact gactcttact tttgcacata tatttctttg aatatggacc aggcatcttt
      481 gaattttaga gcccgggaaa atgtcctgta ttacattttc catcccagac aatccgtctg
      541 tacaaaataa agaaatacgt tcaacccctc ttgatttaag gtctttcaga agttcttccc
      601 atatctcagc ggactcgttt ggtgcaatca tatagccaag aatttctttt gtaccttcta
      661 ctgtgattcc tagtgctata tggaccattt ccctggatac agtatcacgg cgtaaaggca
      721 tggatgttcc atcaagatat acaacggcat actctttgtt caatattctg tttttgaagg
      781 aatctacaca ttccagagct ttgtccgtaa tattagatat ggtctgttta gaatagcgag
      841 ctccacataa tttttggatc atggaacaaa tttctgaatg gctcattcct tcttcaaata
      901 aatccaaaat cgttgtttcg gtaaatagat ctcgtcttct gtatttaggc agaagagaag
      961 tgaaaaattc tcccattcta tctcttggaa tattgagtgt aatagttcca tattttgtat
     1021 caaactttct ttgataggaa ccatttctag aattttcgtt attggttcta gcgtatggtt
     1081 cataatcaag aaaatccgtc aactcatatg cgagtatttc attgagagat ttctccaact
     1141 ctgaacgaaa cagttcggtt acagcctgtt caaaagctgg aagattgttg tcattagaaa
     1201 ataggttttt cattaaagat gtagtaaaat agttcatggg tatattcctt tcgtaaattg
     1261 tttttggtcg aactaatttt actaaggata tacctttttt tctacatttc tagaaaaacg
     1321 aatttacaca agatatttta tgctatccat ttatttttga aactccattt gttttactac
     1381 gtgtattacc tttatttttc tttctatttt taggtgtcta ttatctctta cgttcgctta
     1441 taaatgatga ctcaaaattt aaagcaaaat aaaaataaag gaagtagcct tttgaacaag
     1501 ggcgcttcct ttatttgttg tgggacacca gagtatactt ttttataaaa aataggttga
     1561 tgggaaaccc taagatttcc catcaacact aaatagtata gaaccatatt ttcgttacaa
     1621 ggttaagaat gacgactata gtttttcatc tactaatttt gttttattgc tctccatgac
     1681 tttaaaaaat ggatagtaga tgaagatgct gatgacgatc aacagtaggc aaaagagaat
     1741 attgcgccag tccatacttg aaagataagc ttgtgcaaaa aaaggtgtga aggaaggatc
     1801 gacgataaat ccaatcccaa tccatcctag ttgctgagca aagtaggtca gcaataatga
     1861 aatgatgggc gtaatcaaaa atggaattgc caaaataggg ttaaaaacga taggtaatcc
     1921 gaaaatcact ggttcgttga tggaacagat tccaggaacc aatgacaatt ttccaattgt
     1981 gcgatattcg ggtactttgc ttcgcatcat caataaaacc aaacccaaag taccaccggc
     2041 tccaccaagt acagaaatac ggaacatttg taagttcatt aagtgtgtca tggtttcacc
     2101 atttgccagt tgttcagcat tcaaaccagt ttgagccatt cccaatgtaa agacgatagg
     2161 gaaaataatt gacgagccat tgataccaaa tagccatagg atgttaccca aagtcacgat
     2221 caataagaaa ccacctagac tgccagcgat gttcgttgct ggagtcagga cttccatcac
     2281 cgcttcaggg aagattttgt ttgttgctgc caagaaaatc agattgatgc cataaaagat
     2341 gaatatattt gtcagcagtg gaacgagcat attgataaag gttgctacca tgggtggaac
     2401 tgtatctggg agtttcaact taatattttt tacttcaaat agtcggttga tttccactac
     2461 tagcaagccg atgataattg cgacgaacaa tccgtttgtc ccaaggaaat taagattgat
     2521 ggcaccttct tccactggtg tacaaaccat tagataaatg acagatgcaa ccatgccatt
     2581 catggaagga ttaattttgt attcattggc aagggaaaat gcaatgccaa aaacactaat
     2641 gaatccgatg attcccattg tcaaattata aggggctgtg atgagatcat aatttgctac
     2701 ggcaaaatct ttccagctgg ccataaattt catgaaaata ttagctgtat ctggattgta
     2761 tttatcaata ttgatgggtg gatttgcaaa aatcagaaaa aaagaaccga taacaataaa
     2821 gggtaggcca aacatcatac cgctggatat agcttttagg tggcgctgat tacctatctt
     2881 ggctgcgatg gggctaagta tgtcattcag tttcatgata aacgcagaat tttccatata
//
LOCUS       DACERL010000142         2918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.150, whole genome shotgun sequence.
ACCESSION   DACERL010000142 DACERL010000000
VERSION     DACERL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.150"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(36..1355)
                     /locus_tag="IXF54_002769"
     CDS             complement(36..1355)
                     /locus_tag="IXF54_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1405973.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1352..2005)
                     /locus_tag="IXF54_002770"
     CDS             complement(1352..2005)
                     /locus_tag="IXF54_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1405974.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2293..>2918
                     /locus_tag="IXF54_002771"
     CDS             2293..>2918
                     /locus_tag="IXF54_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1405975.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
BASE COUNT          956 a          475 c          322 g         1165 t
ORIGIN      
        1 tatttttcaa taactattgc aaaattacat cttaattaag aaaatatttt tattttaaag
       61 ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta
      121 cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca
      181 aatattttat ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa
      241 tcattaacta tcccaatatt aattacccca tttacatcag tatattttac cgcattacta
      301 atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt ctcgtctaat
      361 atataattat tgacagttat tttcttttga tttgctaata tttcatactt ttttaatatg
      421 tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt ttctatagaa
      481 tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc aatacattca
      541 ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt ttccagtatt
      601 attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa aaagtcatat
      661 tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc aattgttctt
      721 aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc atcattagag
      781 ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac aatttttatt
      841 tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc agaaaataaa
      901 attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc atctttttgc
      961 atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag ctttatttct
     1021 ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat attatctttg
     1081 atttgtattt cattcttatt atttttttct tttatgaata cttttatttc actactcttg
     1141 gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa
     1201 atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag
     1261 ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt
     1321 atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat
     1381 ttcttatagt agttatacag tctaattgta atttttttct aagttctttt atatatacat
     1441 ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca
     1501 ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg
     1561 catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata
     1621 gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc
     1681 tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa
     1741 atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc
     1801 ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta
     1861 agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt
     1921 gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt atcatattat
     1981 catcttcaac agttaatatt ttcatatata cctccactta agcctataca ctagatatta
     2041 tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt
     2101 tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta
     2161 taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt
     2221 caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa
     2281 aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga taaacattta
     2341 ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt
     2401 aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc
     2461 cgcaacggtt cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt
     2521 ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa
     2581 acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata
     2641 gaactgtacg aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg
     2701 acggtgacac ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt
     2761 ccgacggtgt tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt
     2821 tttatttgtg ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt
     2881 tacaaactaa ctcaatactt cctgcacttt cctagaaa
//
LOCUS       DACERL010000143         2853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.151, whole genome shotgun sequence.
ACCESSION   DACERL010000143 DACERL010000000
VERSION     DACERL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.151"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..997
                     /locus_tag="IXF54_002772"
     CDS             <1..997
                     /locus_tag="IXF54_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405976.1"
                     /translation="KEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVAKINNFTQT
                     NQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKY
                     EKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQDLAEQQMAI
                     EKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTP
                     SPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKE
                     YEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            997..>2853
                     /locus_tag="IXF54_002773"
     CDS             997..>2853
                     /locus_tag="IXF54_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1405977.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAG"
BASE COUNT         1090 a          469 c          555 g          739 t
ORIGIN      
        1 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa
       61 agagcgatac gaaaagaaac gcgacaatgc gtattccttg tgtgtagcaa agatcaataa
      121 ttttactcag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
      181 tattaaagaa cgaaaagaag aattttaccg aggtttgcgt acagataagc taaagattag
      241 caaagaatta aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga
      301 aaaatacgaa aagatccagc aagaattaca agtgcaaatt ccaaaatgga aaaaagagta
      361 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
      421 gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat
      481 agaaaaacaa cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
      541 agaacaaatg gatacattgc gacaagctca gttaaattat ctagcacaac ctcaaccaac
      601 gccttcagta gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc
      661 acagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg
      721 tggaggaaca gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtggc
      781 cacaacgacg tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca
      841 attagaagaa ttattgaatc gtaccaatca gtcaacaaca gaatcaagta tggaaaatag
      901 caaagagagt gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa
      961 cacttccgaa agttcagaat caaatgaggt gaaataatgc gtgttagaga aacaacgaga
     1021 cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat
     1081 actccttcag gagaagagct accagaaaaa catgacaaac gtatttctca aaagattttt
     1141 tatagtggct tagcttatct tcctttaaac tatgttgtta caagaatacc agaagcattt
     1201 catcgatttg agattattcc aggaagttta caaggagtga atatttttca gttacaagaa
     1261 atgccgcagt tattccattt tgattcaact ggtttttgga atcctttgac cccaacaaca
     1321 agtttaggca cacgtttagt tgtttctacg atttgtgttg ttccagctgt acttatggga
     1381 ttgaaaatgg acatgtggtt tcgtcctcta gcagatgggc aaaaaggaga taaccgatta
     1441 aaaacgttaa aggaaatcca acaaatttat ccgcaaatac cagaatcaaa gggaggtttt
     1501 cctggttatg gcggaatacc aattactcat tataaaaagt actggtatat tcagacggat
     1561 acagtaaata cttgtattgt tggtacttcc cgttcaggga aaggacagat agaagtttta
     1621 gcaaccattg acaatttgtc tcgtgcagaa agacaaagtt caatggttgt taatgatcca
     1681 aaaacagaat tatatgttgc tagtaaagat gaactagaag ctagagggta tgatgtgtat
     1741 gcatttaata tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg
     1801 aaatactgga agcgaggaga catagacaca gcgacacagt taaccaatac tttcacgaat
     1861 acgatttatt atgatgccaa cgcttcagac aataaatact ttaacgaaaa tgcgcaaaaa
     1921 gctgtcaatg cgcttatttt tgcgttattg gaacaagcgg atcagacagg ggattactcc
     1981 aaattaacac cgaataatct cgtcgaactt ctttctgaga ttggtggttt taattaccaa
     2041 gatcctgata atgaattcaa gcagctcaat gctttagatg aatttatgaa tcagttacca
     2101 cctggaaaca tagcaaaaaa acaatatggg gcaacaaaaa ttggttctga taaagcgaaa
     2161 ggcaacattc tatcgacagc gatcacagga ctaaatccat ttacactgac gaaaattgcg
     2221 aaaatgactt ctcaatcaac cattgattat aagtccattg gttttccgaa gtatttggat
     2281 ctcaaactac acgagagtct gttaaaccag cgaattgttg tagagtttta tcatcaagga
     2341 aaaaaatttc atgaggaacg tgtcaaagta ggctatagag gtttttgtga aattaacttt
     2401 gattgtcatt tagaacaggg agatcatgtt tgcttaaaat acgatcatcg aggaaaagaa
     2461 tatgttgctt ggtatgagtt ttctcaaaga gtattgaaag acgaagaggg aaatatcgtt
     2521 tatcgaaaaa aacgaggaga aacccatttg ccagaattag aaaaagaagc cgttctaaca
     2581 ttagatgagg ataaaagtaa cttattagta gagtctattc ggatgcacta taccgataaa
     2641 ccaatggcta tttttatgct cacacctgat tatgatccat ctaaccatat cattgttagt
     2701 atttttctta gtcagttgta taaagaatta tctacgcaat gtgttaagac taaaggagat
     2761 aaatgtcacc gtaggattca ttttatttta gatgaatttg ggaatatgcc accaatggat
     2821 aatatggaag gaatcatgac agtcacagct ggt
//
LOCUS       DACERL010000144         2818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.152, whole genome shotgun sequence.
ACCESSION   DACERL010000144 DACERL010000000
VERSION     DACERL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.152"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..354)
                     /locus_tag="IXF54_002774"
     CDS             complement(<1..354)
                     /locus_tag="IXF54_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405978.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDIT"
     gene            complement(813..1070)
                     /locus_tag="IXF54_002775"
     CDS             complement(813..1070)
                     /locus_tag="IXF54_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1405979.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1063..1332)
                     /locus_tag="IXF54_002776"
     CDS             complement(1063..1332)
                     /locus_tag="IXF54_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1405980.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1463..1642)
                     /locus_tag="IXF54_002777"
     CDS             complement(1463..1642)
                     /locus_tag="IXF54_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405981.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2023..2730)
                     /locus_tag="IXF54_002778"
     CDS             complement(2023..2730)
                     /locus_tag="IXF54_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405982.1"
                     /translation="MNKMIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSHNRLDMYKRRPDTLKKGAELLRLETEKHIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMQYQRFERKKGQ"
BASE COUNT          846 a          545 c          365 g         1062 t
ORIGIN      
        1 agttatatct ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat
       61 ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc
      121 aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc
      181 aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc
      241 aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat
      301 tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc
      361 tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta
      421 atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact
      481 agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca gtcatatata
      541 taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa catatctatt
      601 caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg
      661 attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac
      721 actaagctat attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt
      781 aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata
      841 aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac
      901 cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa
      961 cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca
     1021 tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac
     1081 ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat
     1141 tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc
     1201 tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc
     1261 agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc
     1321 tactgcttcc attcctttca ccccttattt atttctataa aacaattgta cattatattg
     1381 tacaattaag caaatgatta atattctaac ttcttataat taaaagtgct atcactttta
     1441 gtttttagga taaaaagtga cgtcactttt tgagtgctgg tacctcaaat cctgcttttt
     1501 cttgggcaat caataaatct aacgcaatac acataatcct ggattcactt ttgccgtact
     1561 tctgctttag ttgctgcaat tctttttgtc tcttttcatc caacgtcacg ggtaaacgtt
     1621 tatttcctat tgcaattggc attagtactc tctcctttca tcgaaataac cctattaaaa
     1681 atatggctgc aatcaacttg aatattagac tcacaagggt taacacaaag cgccaaaaga
     1741 tcaaacaaaa taccagtatt cctaagaata ctacgaagtt taaatgtaga gctgcaccat
     1801 gacgcataag tatctcgcca acaaacagta cgaacaatag aggaatgatt ttattcatat
     1861 ggatctcctt ctttctctta ggttatcggg ctttgcccgc ttatttcgca ccacctaagc
     1921 ggcgggggcg gttggagcga ttggtgcgaa ataagaacca ttcccttaat ttcaaggttt
     1981 tttgttctta tttcacacat cctcactttt cggtataaat ggttattgac cttttttcct
     2041 ttcaaatcgt tgatattgca ttcgtttacg gtcaagttca ttttggattt cttctgtatc
     2101 gtcgtaagtg taggtgtcta aaaaatcagt taaagtcgtg taccagtcgt cgcctgggtt
     2161 atctacttct tctacatatt ccctcaaaaa tgcttcgggt acagcgagtt cataatccat
     2221 tcccattaac acgatagatt ccgtcccaca cgtcaaaaat ctagtaaata caaggtaata
     2281 atcgtctacc gtcgcatctt tgctgtttag ctcttccatt tcttcttcca ttaattccat
     2341 gctctcaaaa attgaataat attctgattc tgagtaatag tgtttcctgc aaatcgtgta
     2401 gctgacaaag ttttcgacca gttcactcct ttctaggtaa acattcatga tatgtttttc
     2461 tgtttctagt cgtagaagct ccgctccttt ttttaatgtg tcaggacgac gtttatacat
     2521 gtctaaacga ttgtggctga ctgtaacctt cgtttgactc gtttcacttt ccaaatacaa
     2581 ttgttcttcc actctttgaa taaagtactt catttcttcc atacaactaa agtctttttg
     2641 aatccgtctg attacttcat tcaaatgttc tttgtttgtt aaaaagtgtg acacatcttc
     2701 cgttgttagt tgataaatca tcttattcat tttcgttgtc ctcctttttc atggtgtaat
     2761 atgcgccata cttattgata ttttcaaaat aatctaacca ttcacttgaa acagtctt
//
LOCUS       DACERL010000145         2722 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.153, whole genome shotgun sequence.
ACCESSION   DACERL010000145 DACERL010000000
VERSION     DACERL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.153"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..236
                     /locus_tag="IXF54_002779"
     CDS             <1..236
                     /locus_tag="IXF54_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405983.1"
                     /translation="HQHDDYGSYSFTLGDVQTYAQTSSVKEEQNLVVFVDYTIGNPQF
                     KDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            247..1053
                     /locus_tag="IXF54_002780"
     CDS             247..1053
                     /locus_tag="IXF54_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405984.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            1076..1306
                     /locus_tag="IXF54_002781"
     CDS             1076..1306
                     /locus_tag="IXF54_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405985.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            1324..1563
                     /locus_tag="IXF54_002782"
                     /pseudo
     CDS             1324..1563
                     /locus_tag="IXF54_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1570..2442
                     /locus_tag="IXF54_002783"
     CDS             1570..2442
                     /locus_tag="IXF54_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405986.1"
                     /translation="MKSTDWIKHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLLSIFTQIVMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGVKEFLMTFVHFVNGHMYLFEVGIILF
                     LVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYYVDFGRYDELINTDIVHTSIHEATVKG
                     IRTTLKDGQETKAIDRRKEVYNFYEVEANLRYGIGKLSLNGRNIPQNKPYIGWKSDAL
                     EVTKKLPMEEEEIQ"
BASE COUNT         1036 a          387 c          520 g          779 t
ORIGIN      
        1 atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttacg
       61 ctcaaacaag cagtgtaaaa gaggaacaaa atcttgtcgt atttgttgat tatacaatcg
      121 gcaatcctca attcaaagat gtcaaaccgc aaaaaataaa aggtggatta actgtcactg
      181 aaaaacaagt agatggtcaa tggaaagtca cagactttag ttatttcact cgttaggagg
      241 aagaaaatga gtaagaaaaa aggagcaaga caacgtttca aaaaacgaaa aagttatctt
      301 agatggctgg ggatctttat gattctcggc tttttgattc ttgctagtcc aatttatacg
      361 cataagaagg tagacatacc agatgagata agcacgaacg catttgtaaa cacgaaggaa
      421 ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcaatc
      481 gacgatgaag atcaaagctt attaaaagaa ctggctaatc ttaattatcg cgtagaagta
      541 aagacagcta aaggggatta tcaagcaata aaagccaaag tgatcaaagt gtctgatgat
      601 tattttgcag tgaagctatc tcatgtacca gaagaatata ttgccatgcg tttaaccatt
      661 attcctgaaa aaattgatcc aaaagtggat atgcaagaac cgcaagatct gatttattac
      721 gttcacgaag ataaggttaa agatcaagtg aaagatgaag attatgaaca acatgcgatc
      781 aattacaaag tgaaaggata caaaaaagaa caaaaagcag cacaaaaaca gattgaatcc
      841 ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga aactaaaaga tcaattgcct
      901 tatcaagttg cagacgatca ggaaaatact gaaaataaga ttaatagtta ccagcaagaa
      961 attgaaacat cgaaaacgca aatgaaagat caagaagaga tcattcaaaa acttcaagaa
     1021 aaaattgatc gtatgacaaa agaaaatatt tagaagaata ggtggagtaa aagaaatgaa
     1081 gcgagcgaac ttggtcggca aagatacttt cgctgagcag aaagtattga ttaaagccta
     1141 tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg aatttatcta acacaacaat
     1201 atctgagttg aagaatggta gaaagagttt agatacctta aaattcagat catttgaagc
     1261 tttgtattta gctgctaaaa aatattttac ggctgaaaga aaataaaaac aggaggattt
     1321 tatgtgaaaa aagtgtatca aaaatgttta tggatcagct atatcaccgt aggtatcgtt
     1381 ggcttctttt ttattgtagc cgtgaccttg ggaacgattt ttctatggtt tactaatgaa
     1441 gatttattat tgaagggact catgagtgta atctcatgcg tcctattgat tttgttgggg
     1501 acagaaatca acaaacttct gccttctcgt tggcaagaaa agaagaaaga aatgagagaa
     1561 gaaattgaaa taaaatcgac cgactggatc aaacacactt ggcttccttt gaaaagaaat
     1621 agcaaacgta aagaattttg gattctacaa ttatggccat taggtaaact ttgtagctat
     1681 ttggctcgtt ttatacttga tttaatcatt tactcgattg tagtatttgg aacggcttat
     1741 tacttattaa atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg
     1801 ttgatttttg tcatgctatt gtctattttt actcaaatag tcatgcaatt taccaggcaa
     1861 aaattaacag caggaaaact tttcaaaagt ctattcgatt taattattgt aaatgcaatt
     1921 ctagcaactt taataaaaca aatcgaacaa tttggaagaa caacaaaagg agtgaaggaa
     1981 tttttaatga cgtttgttca ctttgtaaat gggcacatgt atttgtttga agtgggtatt
     2041 attctctttt tagtcgggta tgtatccacg aaacttatta aacgttcaat tgaaaaagaa
     2101 attgagattg acttgattcc gacacttctt ggttatgagt actatgttga ttttggaaga
     2161 tatgacgaat tgatcaatac agatattgtt cacacgagta tccatgaagc taccgttaag
     2221 ggcatcagaa ctacgttaaa agatggccag gagacgaaag caatcgaccg aagaaaagag
     2281 gtctacaatt tttatgaagt cgaagcaaat ctaagatatg gcattggaaa attgtcgtta
     2341 aatggaagaa atattccaca aaacaaacca tacattggct ggaaaagtga tgctttagaa
     2401 gtaactaaaa agcttcctat ggaagaagag gagattcaat gagtctagtc aatttgaatc
     2461 atttgtttga agatatcagt aatcatttcc aacagttctc aataagagtt tatattattc
     2521 attagtttat tttgattgaa aaaagaagaa aaaatgtaga aagttgtctg tgaaaaaagt
     2581 gtatgctata ctgataatgc aaacacataa aacatttaat actcaaaaag agccctagca
     2641 gttgcagctg cgtaaggctc ttttctttat tcactattaa ccaaataatg aacaggctag
     2701 ctgtctttat catgtttaga at
//
LOCUS       DACERL010000146         2686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.154, whole genome shotgun sequence.
ACCESSION   DACERL010000146 DACERL010000000
VERSION     DACERL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.154"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            59..361
                     /locus_tag="IXF54_002784"
     CDS             59..361
                     /locus_tag="IXF54_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405987.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            358..519
                     /locus_tag="IXF54_002785"
     CDS             358..519
                     /locus_tag="IXF54_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1405988.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            516..821
                     /locus_tag="IXF54_002786"
     CDS             516..821
                     /locus_tag="IXF54_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405989.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            821..1177
                     /locus_tag="IXF54_002787"
     CDS             821..1177
                     /locus_tag="IXF54_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1405990.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1137..1382
                     /locus_tag="IXF54_002788"
     CDS             1137..1382
                     /locus_tag="IXF54_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405991.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1379..1798
                     /locus_tag="IXF54_002789"
     CDS             1379..1798
                     /locus_tag="IXF54_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1405992.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1795..2352
                     /locus_tag="IXF54_002790"
     CDS             1795..2352
                     /locus_tag="IXF54_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1405993.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2349..2645
                     /locus_tag="IXF54_002791"
     CDS             2349..2645
                     /locus_tag="IXF54_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405994.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
BASE COUNT         1004 a          406 c          644 g          632 t
ORIGIN      
        1 ggcggacgtg agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat
       61 ggataagaaa gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa
      121 tgacaaagaa gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag
      181 caacaagcca agaccacgaa aaatcgcaat ttggcacggt gacaaacttc tagtgatagg
      241 gacagctgaa cagttagcaa gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc
      301 aaggtgtggc tacacagacg ctaagaagag aacgtttaga tacgtggagg aatcatcatg
      361 acaacagaag aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg
      421 gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttgcacata
      481 gagaatttaa gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa
      541 tgaaaagaat tgctgaatta accaagtcag aatcttggca agaagacaaa gaaatagttg
      601 cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga
      661 gaaaaattgc aatctggcat gatgaccgaa ttttagtaac aggtactgct gaacagttat
      721 ctgaaattac tggattaagc aaaaacatta tctgggatag agctaggagc ttatggattg
      781 attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca ttacacaata
      841 cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagataaaa aatcaaaaga
      901 acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc gaagtagcag
      961 gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa aagggttcga
     1021 tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt
     1081 caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga cagcaaatga
     1141 taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga gcaacactat
     1201 ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg
     1261 tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg
     1321 agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat
     1381 gataccgaag tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt
     1441 gcattttact aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt
     1501 gaaatcatat cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa
     1561 tggtgtggat atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta
     1621 cttagataat aaagtctgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc
     1681 cactgttgat gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac
     1741 gtacgaagtc accggaaata tatacgagaa tagcgagtta ttggaggaac agcgatgaat
     1801 aaacaggaag caatcaaaaa gttagaaagt attaaagcga taggaaatga tgcaatagct
     1861 gcttgctata acgagagtat aaattcaggt attacgttaa tgaaaaaaat agacgaaccg
     1921 cagaaaccag ttattccaca actcgtggcc ggttggcttg agaaatctac ggaccctttt
     1981 acaaaagctg aaaaaatagc gtatttaatc aaatctaaag atggtgattc atattatttc
     2041 tgtgattggt ttgtacgaga tggcatagtg acgcaagagc aaggagaaga attactcgct
     2101 tgggcaacaa gacaatcata tgaaacacta ttgagcctat acaacggcta cgaggttgag
     2161 aaagagccgt tatgggcaat aaagaatgcc gatggaaact atcttactaa atgtgcttta
     2221 tggggaaaag atggagtaaa ttatagtttt gaatgcaatc catctcatcg attgcttttc
     2281 actgataaag caacagcgga tgctgcagca ttgttggtga atggaacagt ggaagaggtg
     2341 gtagaaagat gaaactaaaa gacggatttt acgctagcag tcacggtatc ggcggtttaa
     2401 tgctagatat gccgacaaag aaccctaaaa cacgcaagaa atcaaaattc aaagtcggtg
     2461 acatggtccg ctgtgaagca gaagagttcg tttatccgtt cagaggatac gttaaaaaga
     2521 tactgtcaaa ctcagcaatc attcgtattg aaaatacaat gaagtgtgat cgttcgactg
     2581 caaaacacaa gcattattta gctgtagcga gattagttga catggaagta atcaagagca
     2641 aataaaaaaa gccggatcgc tccgactgat tcaataaatc caacac
//
LOCUS       DACERL010000147         2686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.155, whole genome shotgun sequence.
ACCESSION   DACERL010000147 DACERL010000000
VERSION     DACERL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.155"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            152..412
                     /locus_tag="IXF54_002792"
                     /pseudo
     CDS             152..412
                     /locus_tag="IXF54_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            575..1240
                     /locus_tag="IXF54_002793"
                     /pseudo
     CDS             575..1240
                     /locus_tag="IXF54_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1276..1521
                     /locus_tag="IXF54_002794"
     CDS             1276..1521
                     /locus_tag="IXF54_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1405995.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1624..2493
                     /locus_tag="IXF54_002795"
     CDS             1624..2493
                     /locus_tag="IXF54_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1405996.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2474..>2686
                     /locus_tag="IXF54_002796"
     CDS             2474..>2686
                     /locus_tag="IXF54_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HAQ1405997.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGV"
BASE COUNT          942 a          383 c          548 g          813 t
ORIGIN      
        1 taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag atatactact
       61 gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg
      121 ggtacaaatt cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa
      181 caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac
      241 tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga
      301 gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt
      361 tgtaaaaatt ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacatgggg
      421 ctgacccaaa agtagctcct atatgaaaaa tagaggtctt tccatttaga tagtagtgga
      481 aagacctcta tttatctttt gaaagtgaat acaaaaataa agcacactct gctataatat
      541 aggtgattaa tccatttgag agaggtgctt tatcatgtat caaaattata ccataaacca
      601 actttgtttg cctatagatc ttgaaattaa attagaagaa aatgattttg ctcatgccat
      661 tgtgcagttt gttgactcta ttcccgatga agtctttctt ccttattatc aatctatggg
      721 tagaccccaa tatcatcctc gtatgctact ttccattatt ctttgtgcct atattcaagg
      781 ggtttattcg ggccgccaaa tacaaaatat gctgatagat agtattcgga tgcgctactt
      841 atctcaagag caattcccta actttcgtac tattaatcgt tttcgcgttc atccgattat
      901 gaacgatatt ctagatcatt catttgttca atttcgtgag ctactggttc aaagtggctt
      961 aatcagtggc agtgctctat acattgatgg tacaaaaatt gaagcggatg cgaataaata
     1021 ctctttcgtt tggaaaaaaa gtattttaaa gtataaggat agccttgatc aaaaagccct
     1081 tgaaaactac caacaaatgg tggaaacaaa aatcttacct gagttaatag atgatttaaa
     1141 ggatgaactt tctatagaag agattgaaag gattcgtcaa tctttggaag aaaaggaaaa
     1201 tcaattaatt gaagcgattg accaaaccga aacagtagaa gggggctgac ccattattaa
     1261 taaggagtgg acaacatgat tgatgtaaat gagctattgg aagaagctat tagagaaact
     1321 gaaaatctaa atgatggcga agtttttctt gttaaagatt tattcaaggg atatgtgggg
     1381 aataggatac ccagaaagga tcgacttctg cttggaacgt tatttttaaa ccatgttagc
     1441 aaaatgaatg gtaatttaaa ggctattgaa aagacttcat ctaatcagca gaggtataaa
     1501 aagacaattg ataagtttta actatctttt gtgaaattaa aaaaattata aatatgttga
     1561 gtagaagcaa aagatttggc ttggaatttt tacagcagtg aatcgataaa aggagcatgc
     1621 ttgatggtag ataatattat taaatcagta gcagagaaat tatcctctct gtcttatata
     1681 gaaggtattg ttttaggtgg ttcacgtgca aggggcaccc atacagagga ttcggatata
     1741 gatatcggca tctattacaa ttcagaatca tttgacataa atactattaa tcaattcgct
     1801 acaaagctgg atgatgagca tagaaataac cttgttgtac ctcccggagc atggggtgat
     1861 tggattaatg gcggcggatg gttagtcata aacgggtatc atgtggattt aattttacgt
     1921 gatattaaac gtgtggaaca aataatgaaa gatacagagc acggaattgt tactgccaat
     1981 tatcagactg ggcatcccca tggttatatt agtgcaatgt atcgaggaga attagcgatt
     2041 agcaaaatac tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca ggcagaaact
     2101 tatcccaatg ctttgcagaa aagtttagtt aactttttta tgtttgaagc agggttctct
     2161 ttaatgtttg taaaagcaaa ttcgggaaca gacgataaat attatattgc gggtcatgtt
     2221 tttcgtatag tttcatgttt aaatcaagtg ttatttgcat gtaataatgc ttattgtatc
     2281 aacgaaaaga aagctataaa actgcttgaa acttttgaac ataaacctga aaaatatacc
     2341 gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg ctacgacatg
     2401 accgagaagc tttataatga agtgaatgaa attgtatcgg agataaataa ctttttaaac
     2461 gaggagagtt cagatgaaag aaaacaaata tgatgataat atattttttc aaaaatacag
     2521 tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga ctttgaaaaa
     2581 gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct atggatggca
     2641 ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgtt
//
LOCUS       DACERL010000148         2667 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.156, whole genome shotgun sequence.
ACCESSION   DACERL010000148 DACERL010000000
VERSION     DACERL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.156"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="IXF54_002797"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="IXF54_002797"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1405998.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          955 a          466 c          593 g          653 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa aacttggaga taacatc
//
LOCUS       DACERL010000149         2563 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.157, whole genome shotgun sequence.
ACCESSION   DACERL010000149 DACERL010000000
VERSION     DACERL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.157"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            59..361
                     /locus_tag="IXF54_002798"
     CDS             59..361
                     /locus_tag="IXF54_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1405999.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNENEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGNKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEESS"
     gene            358..519
                     /locus_tag="IXF54_002799"
     CDS             358..519
                     /locus_tag="IXF54_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1406000.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIVNL
                     RREIRGLEE"
     gene            516..821
                     /locus_tag="IXF54_002800"
     CDS             516..821
                     /locus_tag="IXF54_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406001.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRRIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            821..1138
                     /locus_tag="IXF54_002801"
     CDS             821..1138
                     /locus_tag="IXF54_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1406002.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKSKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILEEYKE
                     DSK"
     gene            1135..1521
                     /locus_tag="IXF54_002802"
     CDS             1135..1521
                     /locus_tag="IXF54_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406003.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFDGDVVQAEQYLTTTIPVRINGIVKYSNRYTMFYLDNGSERHDLYM
                     QSLGGSIYNFEIIGNIYENPELLEEQ"
     gene            1521..2234
                     /locus_tag="IXF54_002803"
     CDS             1521..2234
                     /locus_tag="IXF54_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1406004.1"
                     /translation="MNKQELIEKLAKYVKRYENVMDEHGQGRYCAYEVSLKLAKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGESQKPVMPKLFDDWAKRVIAKH
                     DEFYAISLVARAGWGYGVDFELSENGSSSENKELLYWLVDKCSDNYPNKKKAIEALLY
                     GYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAATERYGAKWKNTFTESEIK
                     AIDERYWPFAVPVEEEAEG"
     gene            2231..2434
                     /locus_tag="IXF54_002804"
                     /pseudo
     CDS             2231..2434
                     /locus_tag="IXF54_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          956 a          384 c          620 g          603 t
ORIGIN      
        1 ggcggacgtg agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat
       61 ggataagaaa gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa
      121 tgaaaatgaa gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag
      181 caacaagcca agaccacgaa aaatcgcaat ttggcacggt aacaaacttc tagtgacagg
      241 gacagctgaa cagttagcaa gtctcacagg cttgcacgag aaaatcgtga gaaaaagagc
      301 taggtgtgga tacacagacg ttaagaagag aacgtttagg tacgtggagg aatcatcatg
      361 acaacagaag aagtgattca aatgcgtatt cgaagccttc aacgtgaaat tgacgatctg
      421 gaacgaacaa aggcagtgat ggtcaatgaa acggcaagga aggcaatcga tttgcacata
      481 gtgaatttaa gaagggaaat tcgtggattg gaggaatgag cgtggataag aaagcgacaa
      541 tgaaacgaat tgctgaatta accaagtcag aatcttggca ggaagacaaa gaaatagttg
      601 cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg aaaacgccga
      661 gaagaattgc aatctggcat ggtgatcgaa ttctagtaac aggtactgct gaacagttat
      721 ctgaaattac tggattaagc aaaaacatta tttgggatag agcaaggagc ttatggattg
      781 attcaaaagg acgacaattt aggtatgtgg aggagaaata atggatctca ttacacaata
      841 cagtgatatt atcctcaaaa aaatcatggc aaagattcag aaagataaaa aatcaaaaga
      901 acgagctgaa ttagttaagt tggaaatggc tgaaacagga gtaggagtgc gaagtagcag
      961 gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa aagggttcga
     1021 tcaaatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt
     1081 caaatttgta gaaaagtata gagagatact agaggaatac aaggaggaca gcaaatgata
     1141 ccgaagttta gagcgtggta cacaccattt aaaggtaaaa caattggaca agaaatgaaa
     1201 tatgggcaag caggaaggtt gatcactcat gctgaaatgg ctccagataa atatgtgctt
     1261 atgcaatcaa caggactgaa agataagaac ggtgtggaga tatttgatgg tgacgttgtg
     1321 caagccgaac aatatttaac aacaactatc cctgttcgta taaacggcat agtcaagtat
     1381 tccaacagat atactatgtt ttatctagat aacggaagtg aaaggcatga tttatatatg
     1441 caatcattag gaggtagcat atataacttt gagatcatcg gcaatatcta cgaaaatcca
     1501 gagttattgg aggaacagtg atgaataaac aggaattgat tgagaaatta gctaaatatg
     1561 taaagcgtta tgagaacgtt atggatgagc atggccaagg aaggtactgc gcttatgaag
     1621 tatctttaaa gttggcgaaa agactaaatg aatcaaaaat tacagacgaa caagcttgga
     1681 ataaggtagc tgaggcttat cctgaatcgg cacaaagctt gagaaacact ttagataatg
     1741 ctgtatttgg taagactggt gaatcgcaga aaccagttat gcctaagctt tttgatgatt
     1801 gggcaaaacg agttatagca aaacatgacg agttttatgc tatttcactt gttgcacgtg
     1861 caggttgggg atatggtgtt gattttgaac ttagcgagaa cggatcatca tcagaaaaca
     1921 aagagctgtt gtactggctt gttgataaat gcagcgacaa ttatcctaat aaaaagaaag
     1981 caatagaagc tttgttatac ggctacgagg tcgagaaaga accgttgtat cacgtgaagc
     2041 taaaaatacc aggcgtaaca tactacctta tccaaacttt tttaatgcca ggagatactc
     2101 atatgtgttt ctcagcggct acagaacgtt atggggctaa atggaaaaat acttttacgg
     2161 aatcagaaat aaaagcaatt gatgaaagat actggccatt tgctgtgcca gtggaagagg
     2221 aggcagaagg atgaaaaaat tgcaccaact catatctgag aaagaaagcg aactccaaaa
     2281 tttagaagat agtttgggat tggggtttcc aattgttgag caagctaaaa tgactcaaat
     2341 cagtcatcta cgacttgaat tggaagattt aagacaaata gaaaaatcaa ttcaactcaa
     2401 cgacaaccag caaatcgtgt ttgagtggtt gaaactaact gcgccaacag gaaagccaat
     2461 gcaagttgta ttttggatga tgaataatgc agcatggggg catttagacg aattaaggga
     2521 tcctttgatg gaattaacag acaaagagca attcgaagtc cta
//
LOCUS       DACERL010000150         2510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.158, whole genome shotgun sequence.
ACCESSION   DACERL010000150 DACERL010000000
VERSION     DACERL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.158"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..225
                     /locus_tag="IXF54_002805"
     CDS             <1..225
                     /locus_tag="IXF54_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406005.1"
                     /translation="KDTKIEEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQ
                     ATNMQNPTKKETALNYLKSVKEWLDQQQQN"
     gene            complement(346..432)
                     /locus_tag="IXF54_002806"
     CDS             complement(346..432)
                     /locus_tag="IXF54_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1406006.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            1077..1814
                     /locus_tag="IXF54_002807"
     CDS             1077..1814
                     /locus_tag="IXF54_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1406007.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            1842..2381
                     /locus_tag="IXF54_002808"
     CDS             1842..2381
                     /locus_tag="IXF54_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1406008.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
BASE COUNT          959 a          373 c          455 g          723 t
ORIGIN      
        1 aaagatacaa agatagaaga agcctgtagt gatctatttg gagacacaaa cccttggttt
       61 gccagtgaag aagaaaaaaa agaaaaacaa caatctgtca catatgaacg ctatgaaatc
      121 ttacttcaac aagcaactaa catgcaaaat ccgacaaaaa aagaaaccgc cttaaactat
      181 ctaaaaagtg tcaaggaatg gttagatcag caacaacaaa attgaatttg atttaaaatc
      241 atgttaaact gaatgtgcaa atgatcaaac attagataca cgaaagcccc agagaggtcg
      301 gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact tcttgtttcg
      361 gttattcaac caataactga ataaagagac acaaactcct acaagaaggg ggcaaatgaa
      421 cagttcaaac attagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt
      481 attatatctt aaaaaattaa atttgtgtgg tgtttttaag tatcacttgt tttagggttg
      541 ttagggcttg ccctgaccgc ctgtaaggcg cttctcgact agattaaggg aaattaatca
      601 agaattgtct tagaacggct tagacaagct ttcagagtct cacacctcct ctttgaacaa
      661 cggttttagc aaaggagccg gaggcgactg cgcagtataa tatgagagca tagcgatcac
      721 acctttttag cgaccgtagg gagcaagttt tccagtttag aaaaattcgc gaaccttttt
      781 ggaggaaaaa gtaggagttt tttaggcggg aaattcaatg tgctcttaag atatactgtt
      841 agatatactg ttagatatac tgttaataat aactgttagg gattagcaac cttatgggag
      901 agtaagtatt gacataggta gtgggggata aattgtcaca ctatggggga taaattgtca
      961 cactatgggg gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa
     1021 ttgtgtttta catcctctta tgtctatgat aacataattt attgaggtga tttttattga
     1081 gtaacgatat agttcgatat aataatggat taaattctgt ccctttaaga aaatttaccc
     1141 ctatagatat ggatttattt tggggaatat gttctaaaat gaaaagaaaa ggtacaaaag
     1201 aagaaatttt tacatttaaa caaataaaag aattaattga ttacaatata aatgcacgtg
     1261 gcgaccgttt tgctaatgat ttaataaaaa tgtcaaaaaa aatcattact ttgtctttta
     1321 tttatgaaga cgaaaaaaca tatgaacaat taaatctgtt tcaaaaattt aaaatcgata
     1381 aagtgaatga aacattatta ataaaggttt ctgaagaatt tgaatatata cttaattcaa
     1441 tcggaactaa ttttactcgt ttcgaattag aaaatatgac aaacttaaag agtagctacg
     1501 ttaaagagtt atatcgccaa cttatggctc ataaagacag aagatctaaa aaaggtcact
     1561 ggtttgtaaa aatagaagat tttagagctc tattagcaat accagaaagt tatcgcatga
     1621 gagatattga taaacaaatt tttgcccaag cgaaaaaaga atttttaaac gttaattcaa
     1681 ctcaccctgc tatttttaaa acttttgaaa tcaaaaaaat taaagcctac aaaggaaata
     1741 agataggatc tctgcaaatt tattttgaag aaaatatacc ttcaattacc ttagataatt
     1801 ggttagagaa ataataccta acaatttagg aggcaattat catgcaaccc aaagaaacct
     1861 ttacaataaa ggaattatct gaattatttg aaataagtag gcaagcaatc tctaaacatg
     1921 ttgataaatt agacccgtcc ttattagcta aaaatgagcg tggttataag gttgtattac
     1981 gttctggagt attgcaactt gctagcaact taggtaacca aaggttacta tataggttgc
     2041 atgagtatga acaagaaaac gataaaaacg tttatatatc aagcaaaaac gtgattgtaa
     2101 acgagttgct agataagttg cgtgaagaaa atgaattttt acgagaacaa ctttccctca
     2161 aagagcaaaa taattctaat atgcaaaaat tactcgacca gcaacaacag ctcaccctac
     2221 aggcaaatca acagatcgaa aaactgcaag aacagttaca attagcttat acggaagaaa
     2281 ctccagaaga tgaatcaact actattcttt ctgaagaaac agaatcgata gaaaaacaca
     2341 caaaaaagaa gaagcaaaaa tggtggcaac tttggaaata actggaggga acattcatga
     2401 caagaaaaaa tcttctattc ggttctattt tactaggaat tctaggcttc agtccgatgt
     2461 tattgtcagt aatcaataaa gggaataaac atggtgaatt gaaattgaat
//
LOCUS       DACERL010000151         2484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.159, whole genome shotgun sequence.
ACCESSION   DACERL010000151 DACERL010000000
VERSION     DACERL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.159"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1015..1305)
                     /locus_tag="IXF54_002809"
     CDS             complement(1015..1305)
                     /locus_tag="IXF54_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406009.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IXF54_002810"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IXF54_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          459 c          348 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACERL010000152         2464 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.160, whole genome shotgun sequence.
ACCESSION   DACERL010000152 DACERL010000000
VERSION     DACERL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.160"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..1023
                     /locus_tag="IXF54_002811"
     CDS             <1..1023
                     /locus_tag="IXF54_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1406010.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..>2464
                     /locus_tag="IXF54_002812"
     CDS             1157..>2464
                     /locus_tag="IXF54_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1406011.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT          971 a          314 c          469 g          710 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tatt
//
LOCUS       DACERL010000153         2399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.163, whole genome shotgun sequence.
ACCESSION   DACERL010000153 DACERL010000000
VERSION     DACERL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.163"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            208..2070
                     /locus_tag="IXF54_002813"
     CDS             208..2070
                     /locus_tag="IXF54_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1406012.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="IXF54_002814"
     CDS             2070..>2399
                     /locus_tag="IXF54_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1406013.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DACERL010000154         2364 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.164, whole genome shotgun sequence.
ACCESSION   DACERL010000154 DACERL010000000
VERSION     DACERL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.164"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            252..530
                     /locus_tag="IXF54_002815"
     CDS             252..530
                     /locus_tag="IXF54_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406014.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            569..991
                     /locus_tag="IXF54_002816"
     CDS             569..991
                     /locus_tag="IXF54_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406015.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            1011..>2364
                     /locus_tag="IXF54_002817"
     CDS             1011..>2364
                     /locus_tag="IXF54_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406016.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     Q"
BASE COUNT          958 a          328 c          468 g          610 t
ORIGIN      
        1 taaagagcat aaggaaacgt gtacccactt tcaaaataaa gtgggtagat aggctgtgcc
       61 tatcacgttt ctgaaaaaaa taaatgaccc caaaattttc aaattatgtg taggtttatg
      121 ggtagcaaaa atatgaatgt gggtagcaca aaagttcatt tgtgggtagt aaaatttaaa
      181 tatgtgtagc aatgtgtgta gctacacaca ttgctacaca cgttataaca tatagtcaaa
      241 aggagatatt tatggaagta aaaataagag atattgatga aaaaacgatt caaagaattg
      301 accgaagatg tattgaatta agtgaacgta ctgggactaa atggagccga aatcgatacc
      361 taaaagtttt gatcgaaaat gattttgatc acgctcttct ggattataaa aaagatcaat
      421 ttgatcggtt gttagaaaaa tttgtggata ttcaaactta taatacgaaa attttagaag
      481 aatatattgc aacaaataat caattaatcg ccttgttaat tgaacagtaa aaataatatt
      541 gtgaatttat aaaaggagga aatcaacttt ggcgaaagca acaagaaaac atatttatct
      601 ttatgatatg catatcgaag tacttgatga gataatttct actcatgacg atatatccaa
      661 ttatagtgaa gcagttagac atttgtgtat gatgtatgat cccaaaactg aagaaaagat
      721 aagtcccagt caaatcaaac taaatgcaat tggaaaagaa gtttctattt taactgaaat
      781 ggtttcggag tttgcagatc ttcatttaaa agatacagga atattaattg gtacaaattc
      841 aactgtttat caagaagcga aagagcgtgt ggaaggcaag ataaaagcaa atcaaacgaa
      901 gaaatattct cctaagataa agaaaacaat aagcgaatca gtgactacaa aaagtgaagt
      961 atttgatcca acaaggtttt taaaacgata aaaattagaa ggagaacgtg atgagtaaat
     1021 cacctgcagt cattctcact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
     1081 acattgatta tatgacaaga gaaaaagcgc tggaagaaat ggaacggcgc acgctcgaag
     1141 aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag
     1201 aaactttttc ggagagtaag acggataaag aattcagtga aacgtcgaat gaagcgcaat
     1261 cgttgatgga atatggaatc aattttgaca agttagagga acaagatttt accaaatatc
     1321 tttcttatat gacgcgtcac tatgcgttag aacaaaaaaa ggaactaacg gaaagggaaa
     1381 aaatagaata tcaacgtttg aatcaagcaa tggaaaaata tcaagcagaa ccacacaaaa
     1441 aaacttcagg aactcttcct ggagtttttt ctatatcttc agacgaagta aaaggaagtg
     1501 atctgaagga gattaagcag atcttccaaa gagggcaaca aaaaggctca atcatttatc
     1561 aagatgttgt ttctcatgat aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa
     1621 cagatacttt agatgaagtt ggactaaaag ctgctggaag gaaaatgatg gagacgttgt
     1681 ttgagaaaga aaaactgaat gatacgggat attggatggc taccattcac cgtaacacta
     1741 agcatattca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccatacactg
     1801 aggtcgaaaa tggtgtgagt tatatcgtac cgaaaggaaa gagaagacaa gcaacattag
     1861 acgcaatgaa gaccaattat gtgcatgaat tagaacgttt ttcttatgaa aaggatcgaa
     1921 tgataggcat ggagaaacga aatttattaa cgcggaaaag tgatttgcgt aacagcttaa
     1981 caaaagtggt aaaagagcca gtacgttatg atcgacacgc aatgaactta ttaaatgaag
     2041 tatataaaac cttacctgac aaacgttcgg aatggaatta tggggatgaa aatcgaacta
     2101 aactttcacc atacacaaga gagaaactcg acgagttaac ccaatatgtg ttaacaaaag
     2161 aacctgaata ccaagaatat atacagattt caaaagcatt gaaagaagaa gcaaaaagtc
     2221 tttatgggga aagcaagaga gaatcaaagg atgcagagaa aaatgctctc tttgatttaa
     2281 aaaagcgcac aggaaatgcc atcctaacag agctgaagag acaggatagt caaataaaac
     2341 cattaataga aagtgttgat caga
//
LOCUS       DACERL010000155         2358 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.165, whole genome shotgun sequence.
ACCESSION   DACERL010000155 DACERL010000000
VERSION     DACERL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.165"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..669)
                     /locus_tag="IXF54_002818"
     CDS             complement(<1..669)
                     /locus_tag="IXF54_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
                     /protein_id="HAQ1406017.1"
                     /translation="MTYTHLTSNKLAMIEAYYNNHQSVAKTAVLLNRSRQTIHKVYQF
                     FKTGHNALDYFNQYKKNKTRCGRRPIVLSDKQTEYIQKRVVQGWTPDVIVGRAEFSIS
                     CSMRTLYRMFKQGVFEVTHLPMKGKRKANGHKETRGKQSFRRSLRDRGNDYSKFNQEF
                     GHLEGDTIVGKKHKSAVITLVERLSKVIITLQPEGRRAIDIENRLNQWMQSVPKHLFK
                     SMTFR"
     gene            complement(751..2205)
                     /locus_tag="IXF54_002819"
     CDS             complement(751..2205)
                     /locus_tag="IXF54_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1406018.1"
                     /translation="MFSKEESSMGYKELGKDILANVGGSENVSSLAHCATRLRFNLKD
                     DSKADESAIRNLKGVVGVVNQGGQFQVIIGGDVKFVFAEIDKLGDFSASKESSSEANN
                     KGIVSKVLDTIAGIFVPIVPALTGAGMLKALLALLVMLEWMTPESQTYQFLNFIGDAA
                     FYFLPVLLASSAAKKFGCNQYIAITLGAILLHPTFTAMIAGARETGESLALFGLPVTL
                     ANYGSSVIPIILAIWFMSYIEPIIDKLMPNAVRIFMTPLLTLLIVAPITLIVIGPLGT
                     FLGNGLGSLIEFVNTYASWLVPLLVGAFTPLLVMTGMHYGLIPIGINMLATSGFDTVA
                     GPGMMVSNIAQGGAALAVAFRTKQLSVKQLAVSSGLSAFMGITEPALYGINMRFKKPL
                     IASMVGGGAAGLFIGIMGVGRYAQVAPGILALPSFFGEKGLSNFIYAAIGCAIAFVVG
                     FIASFILGIDEETEEVKEDTSEIPVNIASDNRRL"
BASE COUNT          764 a          486 c          372 g          736 t
ORIGIN      
        1 gcgaaaagtc attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg
       61 attttcaata tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa
      121 tcgctcaacg agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc
      181 aaggtggcca aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg
      241 acgaaaagat tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat
      301 aggtaggtga gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga
      361 acaagaaata gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac
      421 cctcttttga atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg
      481 agttttattc ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa
      541 ttggtaaact ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac
      601 agattgatga ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt
      661 ataggtcatt tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat
      721 aaatgacttt tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt
      781 tactggaatt tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat
      841 gaatgaagca ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa
      901 atttgatagt cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg
      961 agcatatcga ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat
     1021 tgaagcaatt aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat
     1081 tcccataaaa gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt
     1141 tctaaaggca actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg
     1201 accagcaaca gtatcaaatc cagatgttgc taacatatta ataccgattg gaattaaacc
     1261 gtaatgcata cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa
     1321 ccaacttgca tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt
     1381 acccaaagga ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg
     1441 cgtcataaat attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga
     1501 cataaaccaa atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac
     1561 aggtaatccg aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt
     1621 aaaagtcgga tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa
     1681 tttttttgca gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat
     1741 gaaatttaaa aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag
     1801 agctaataat gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat
     1861 acccgcaatt gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact
     1921 ttctttagaa gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc
     1981 tccaccaata atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag
     2041 atttctaata gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt
     2101 tgcacaatgc gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt
     2161 tcctaattct ttatacccca tgctactttc ctccttagag aacattttct cttttgataa
     2221 tttgaatata gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt
     2281 tgcgaaattc attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc
     2341 cacttactaa ctaatcgt
//
LOCUS       DACERL010000156         2349 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.166, whole genome shotgun sequence.
ACCESSION   DACERL010000156 DACERL010000000
VERSION     DACERL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.166"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..543
                     /locus_tag="IXF54_002820"
     CDS             <1..543
                     /locus_tag="IXF54_002820"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1406019.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="IXF54_002821"
     CDS             complement(1016..1588)
                     /locus_tag="IXF54_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1406020.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="IXF54_002822"
     CDS             complement(1604..2209)
                     /locus_tag="IXF54_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1406021.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          358 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtcta
//
LOCUS       DACERL010000157         2272 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.167, whole genome shotgun sequence.
ACCESSION   DACERL010000157 DACERL010000000
VERSION     DACERL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.167"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXF54_002823"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXF54_002823"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1406022.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IXF54_002824"
     CDS             complement(1098..2201)
                     /locus_tag="IXF54_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1406023.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
BASE COUNT          680 a          488 c          404 g          700 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt tt
//
LOCUS       DACERL010000158         2217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.168, whole genome shotgun sequence.
ACCESSION   DACERL010000158 DACERL010000000
VERSION     DACERL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.168"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..105
                     /locus_tag="IXF54_002825"
     CDS             <1..105
                     /locus_tag="IXF54_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1406024.1"
                     /translation="EYMGDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            118..1017
                     /locus_tag="IXF54_002826"
     CDS             118..1017
                     /locus_tag="IXF54_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1406025.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            1020..1517
                     /gene="ssb"
                     /locus_tag="IXF54_002827"
     CDS             1020..1517
                     /gene="ssb"
                     /locus_tag="IXF54_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1406026.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            1883..2143
                     /locus_tag="IXF54_002828"
     CDS             1883..2143
                     /locus_tag="IXF54_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406027.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT          816 a          357 c          436 g          608 t
ORIGIN      
        1 gagtacatgg gagatccatt gagagatcca agcaaattaa gagattatat gacaaaacta
       61 catgtagaaa aagaaatgaa ataccatatg caaagcttat attgatggag gaaaaagatg
      121 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
      181 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
      241 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
      301 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
      361 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
      421 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
      481 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
      541 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaagtggag
      601 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
      661 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
      721 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
      781 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcagagg aaatgactat
      841 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
      901 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
      961 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     1021 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     1081 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     1141 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     1201 tggcgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     1261 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     1321 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     1381 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     1441 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     1501 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     1561 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     1621 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     1681 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     1741 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     1801 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     1861 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     1921 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     1981 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     2041 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     2101 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     2161 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaag
//
LOCUS       DACERL010000159         2214 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.169, whole genome shotgun sequence.
ACCESSION   DACERL010000159 DACERL010000000
VERSION     DACERL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.169"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..134
                     /locus_tag="IXF54_002829"
     CDS             <1..134
                     /locus_tag="IXF54_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406028.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            131..1009
                     /locus_tag="IXF54_002830"
     CDS             131..1009
                     /locus_tag="IXF54_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1406029.1"
                     /translation="MTNHKRKNCVIPLAISMSLLIYTHVLSTFMSVIVIGFLFIGSLF
                     SKRKEKKEMSDLILNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNI
                     SDSLIGAVNNDLVTFTMGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFP
                     WEILQKTPIQIIQFPWRILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTV
                     TAATEANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVK
                     VGDRWRMHLIKHIIPK"
BASE COUNT          801 a          297 c          339 g          777 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataaaagaaa gaactgtgtg attcctttag ctatcagtat
      181 gagtttgcta atctataccc atgttctctc cacttttatg agtgtgattg ttattggctt
      241 cttgtttatc ggctctcttt tctctaagcg aaaagagaaa aaagaaatgt cggatctgat
      301 attaaatatg ataaaagctg ttggttggac cattctttta acggcttacg tttggtatcc
      361 gatgttacaa caagtcctac atcaaaaaat taatcgtcct tttcgcacaa atcttcaaga
      421 aagggcattg aatatttctg atagtttaat cggtgcagta aataatgatt tagtcacgtt
      481 tacaatggga acaattggtg tggtttcatt gattcttcca cttttctttt taaaaaaatt
      541 taccaataaa gaaaagatta tttatttttg tgcagtgacc acttggtttt tatcaacgaa
      601 tctttttcca tgggaaattt tacaaaaaac acctattcaa atcattcaat ttccgtggag
      661 aatcttgggt tttcaagtgc tttttggttc attaatctta accatagtgt ttttaaagtg
      721 gaaaacaagc aacaaaaaaa gtatgtggag ccttgtagga atagttctta ttatttttac
      781 tgtgactgca gcaacagaag caaattattc gcataaaatt cagtcgtata aaggtcgttt
      841 agttatgacc aaaaaagatg ttacacgtta tacatcctca agaactggag gcctatatga
      901 ctatgcacca ttagatgcat tgaaatataa agatcattta aagaaacatg aagtaaaagt
      961 tggtgatcga tggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     1021 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     1081 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     1141 acgggaaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     1201 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     1261 ggttaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagcaa
     1321 tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
     1381 cgaaaagtga aaagagcaaa tataaaattt aataaaaata tagaactaaa taggaaaacg
     1441 tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga aaattatata
     1501 aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga taatttttag
     1561 gtctatttaa ctcatcagac attctatgct catttttctc caaatttgtt tcaactcttt
     1621 cgtacttaat tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag
     1681 tatgatatat atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct
     1741 aaaacctata aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat
     1801 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
     1861 ttattgattg caaaattatg tgacaaaaag aactagtaga agaaggaatg atggcttgat
     1921 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
     1981 ctatatttta ggtttcatat tatataaatt aataatatac taccatttat tggtagtata
     2041 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaggaaagtt
     2101 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
     2161 gtgatataac gaaatatcta cacagacaaa attatgatct tgcgtaaagt tata
//
LOCUS       DACERL010000160         2189 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.170, whole genome shotgun sequence.
ACCESSION   DACERL010000160 DACERL010000000
VERSION     DACERL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.170"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(325..528)
                     /locus_tag="IXF54_002831"
     CDS             complement(325..528)
                     /locus_tag="IXF54_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406030.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IXF54_002832"
     CDS             complement(987..1952)
                     /locus_tag="IXF54_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1406031.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACERL010000161         2100 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.171, whole genome shotgun sequence.
ACCESSION   DACERL010000161 DACERL010000000
VERSION     DACERL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.171"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            6..980
                     /locus_tag="IXF54_002833"
     CDS             6..980
                     /locus_tag="IXF54_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1406032.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="IXF54_002834"
     CDS             1710..2027
                     /locus_tag="IXF54_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406033.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
BASE COUNT          744 a          257 c          338 g          761 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga gggaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
//
LOCUS       DACERL010000162         2044 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.172, whole genome shotgun sequence.
ACCESSION   DACERL010000162 DACERL010000000
VERSION     DACERL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.172"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            25..840
                     /locus_tag="IXF54_002835"
     CDS             25..840
                     /locus_tag="IXF54_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1406034.1"
                     /translation="MMEKLLERIVESPYHDDFVLKGGFLIGSKYGLENRSTVDIDTTY
                     RNSKLTKEKLETVFSELTADPTKDGIRFELKGLTETREADYYPGFQAKFIAYLENARV
                     PFKLDITSGDSIIPAALMYEHKLMFEDKTIHIPAYPTEQILAEKLHATFSFGKDNSRM
                     KDFYDLYMIPKLERINEKNLYKSVQGTFKERNNQSVFKHLYDQEMPQLVKDEGLREKW
                     KRYQQENYFAKDISFESTLTSVDSLMQSIVEQEKVERKQNFKRSQQMNQEMER"
     gene            843..1328
                     /gene="ssb"
                     /locus_tag="IXF54_002836"
     CDS             843..1328
                     /gene="ssb"
                     /locus_tag="IXF54_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1406035.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            1373..1606
                     /locus_tag="IXF54_002837"
     CDS             1373..1606
                     /locus_tag="IXF54_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406036.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(1765..1887)
                     /locus_tag="IXF54_002838"
     CDS             complement(1765..1887)
                     /locus_tag="IXF54_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1406037.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
BASE COUNT          706 a          351 c          407 g          580 t
ORIGIN      
        1 ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaacgtat tgttgaatcg
       61 ccttatcatg atgattttgt attaaaaggg ggatttctga tcggatcaaa atacgggtta
      121 gaaaaccgtt caactgttga tattgatacg acttatcgaa attcgaaact aacaaaagaa
      181 aagttagaaa ctgttttttc tgaattaaca gctgatccca caaaagatgg aattcgattt
      241 gaattaaagg gattaacaga gacgagggaa gctgattatt acccaggttt tcaagcaaag
      301 tttattgctt atcttgagaa tgcacgtgta ccatttaaat tagatattac ttcaggtgat
      361 tctattattc cagcggcatt gatgtatgaa cacaaattaa tgtttgaaga taaaacgatt
      421 catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc cacgtttagt
      481 tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat tccgaaatta
      541 gaacgtatca atgagaaaaa tttgtataaa agtgttcaag gaacattcaa ggaaagaaat
      601 aatcaatcgg tgtttaagca tttgtatgac caggaaatgc ctcaattggt gaaggatgag
      661 gggttgaggg aaaagtggaa aagataccaa caagaaaact attttgccaa agacatttct
      721 ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga gcaagagaag
      781 gtagaacgaa aacaaaattt taagagatca caacaaatga accaagaaat ggagagataa
      841 agatgattaa taatgtaact ttagtaggac ggttaaccaa agatccagat ttacgttata
      901 ccgcaagtgg cacagcagta gcgactttta ccctagccgt caaccgaaat ttcgcgaatc
      961 aaaacggcaa tcgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa
     1021 caatggcgac attagctaaa aaaggcggct tgatcggtgt tgtcggacgg attcaaacac
     1081 gaacttatga caaccagcaa ggacaacgtg tgtatgtgac tgaagttgta gcagataact
     1141 ttcaactatt agaaagtaaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca
     1201 ccagtccaag caccgccacc tttgaacaac gggatacagc gacgcctaac aacaatggat
     1261 tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac
     1321 cgttttagtt cgagaaagaa ggagtaagca tggcaaaaaa aatagtctga cgatggatct
     1381 attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg aaaatccttt
     1441 ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg tatgttctta
     1501 tcttttaaaa agagcaggaa gggataattc cttttggcga ttgactaaag tcacaatagc
     1561 agcgacagct gttatactta cggctattct accgctttat atgtagggtt aacgggaaat
     1621 cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt tgcgctaaca
     1681 acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat ttggaaataa
     1741 ccgctgacct gctaaagtgg acggttattt ctttttgtca tttttaagca tatttacaat
     1801 caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa ttgtctcata
     1861 tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata agcaccacca
     1921 cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat agcatatcta
     1981 aaatcgaata aaaattctca attttggttg tttttgctat aattttagcg aggtgattgt
     2041 gatg
//
LOCUS       DACERL010000163         2034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.173, whole genome shotgun sequence.
ACCESSION   DACERL010000163 DACERL010000000
VERSION     DACERL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.173"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXF54_002839"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IXF54_002839"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1406038.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IXF54_002840"
     CDS             complement(1431..1823)
                     /locus_tag="IXF54_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1406039.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACERL010000164         2027 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.174, whole genome shotgun sequence.
ACCESSION   DACERL010000164 DACERL010000000
VERSION     DACERL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.174"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..901
                     /locus_tag="IXF54_002841"
     CDS             <1..901
                     /locus_tag="IXF54_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1406040.1"
                     /translation="LDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEYSGSISQF
                     LAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFTEKNDLI
                     SVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYVEAAIL
                     CNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLS
                     EVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
     gene            895..1317
                     /locus_tag="IXF54_002842"
     CDS             895..1317
                     /locus_tag="IXF54_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406041.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1619..1999
                     /locus_tag="IXF54_002843"
     CDS             1619..1999
                     /locus_tag="IXF54_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1406042.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT          733 a          350 c          366 g          578 t
ORIGIN      
        1 gctcgatgga gaaaatacgg caaatagttc tattttcttt ccagtatttt cagacgctga
       61 taatacgatt aaatatattt ataccgtcaa aaaaaataca aatggagaat attcaggaag
      121 tatttcacaa tttttagcaa aagaacttca aaatctagta gatagtaata taacagatac
      181 aaccttattt tttcaagata atagcattta ttattccgat aatggacaaa ttaaactact
      241 ttggaaatca cctttaagta cagaaaaaaa tggtgtttct tcctttacag aaaaaaatga
      301 tcttatatcc gtaaatataa aaaataaaat taaaaatttc tctaaaaaac aaaattttcc
      361 tgcggttaaa cgtgtaaaaa gtgtctcctc cggagataac tttattatga ttaattggcg
      421 tatagcagaa acccaaacaa gttttccgtg gtgtgctgct tacgtagaag ctgctatctt
      481 atgtaacaaa acagatgtac atgctacttc agctagagcc attatgcatt atacctatcc
      541 taatttaaac aacagtcaaa ttgaatcaaa aagtcttaat caagatcaaa taataaaata
      601 tgcaaattac tgtggtgttt atcctaaaaa agtagcacga atactaaacc tttcagaggt
      661 ccaaacacaa ctaagaaaag gaaatgccat ttatatggga actgctggtt atggaaatta
      721 tagtaacagc cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg gttatcaaac
      781 ttactatgtg tggaatcctt ggtataacta cccaactgta ataagtgcaa atgcaggacc
      841 agttacaatt cctgtacctg gaggtggttt tacttgggac aataccatta caaattggta
      901 aataaaccca ttgctttagt tatgggtcta gtgttagttc ttttcttaac tagttgttca
      961 aaaaaacaag aaatacaaaa gactgatctg attccttttc taggaacttg gcaaactaaa
     1021 gatcattcag aacgtctagt aatcactaat ttaaatactt caaaaaatat agaacaatat
     1081 accatcttag taaaagagaa caatcataaa attcaaatca ttgataaaaa tttaaatata
     1141 aatgaacaag ttttcattta caaagcaaca aataatttac taacactgcg ctctaataaa
     1201 aaacaattag attacacttt tagtttggat tctccaaaaa agctcgtatt tattttgaca
     1261 acgaatgatg ggacagaagg gacagctaaa ccaattgaat tttttaaagc tgattgatga
     1321 atagttgttt gatattacaa aacaataatt tcaccgaagg tgaaacaagc gaaactttac
     1381 aaaaagtggc tagcatttgc tagccttttt tgtattgtgg agcgtgggtt ttgaggggga
     1441 ttcaaggggg agcttgcgcc cctttgaaaa gcggttattt gcaccaaata cgtaggtatt
     1501 tggctagcaa atatagagct tgccaagccc ttttggtgag ctttactcat tgtgcctttt
     1561 taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg tgggacttat
     1621 gagcgaacaa aatcaaaatt tggctagcga tcattctaaa aaatatacct atcgatccaa
     1681 gccaaaacaa atcagttttc gagtgagcga atctgaattt gcaaagctga agcagtcagc
     1741 tgaagctttg caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct
     1801 ggtgacaccg aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc
     1861 tggagggaat atcaatcaaa ttgccaagtg gtgcaataca caccaacatg acgttgctcc
     1921 tggagacgcc caacgtttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat
     1981 atggcaacag ttaaagtaag tcgaaccact tcctgtagcc gagcaat
//
LOCUS       DACERL010000165         2020 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.175, whole genome shotgun sequence.
ACCESSION   DACERL010000165 DACERL010000000
VERSION     DACERL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.175"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            100..570
                     /locus_tag="IXF54_002844"
     CDS             100..570
                     /locus_tag="IXF54_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1406043.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IXF54_002845"
     CDS             792..1895
                     /locus_tag="IXF54_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1406044.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DACERL010000166         2003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.176, whole genome shotgun sequence.
ACCESSION   DACERL010000166 DACERL010000000
VERSION     DACERL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.176"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..378
                     /locus_tag="IXF54_002846"
     CDS             <1..378
                     /locus_tag="IXF54_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1406045.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IXF54_002847"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IXF54_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1406046.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IXF54_002848"
     CDS             1859..>2003
                     /locus_tag="IXF54_002848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1406047.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACERL010000167         1993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.177, whole genome shotgun sequence.
ACCESSION   DACERL010000167 DACERL010000000
VERSION     DACERL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.177"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            87..386
                     /locus_tag="IXF54_002849"
     CDS             87..386
                     /locus_tag="IXF54_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406048.1"
                     /translation="MNGQTPQTPQTPVVEESTQQSQQTETTTEQVKLYGEVLTDPHEK
                     AEMIRDLYEGNQGFNRDILNSIPDQEIIDSFVGNATNAQLAQSTTNLLEKYPNLK"
     gene            988..1593
                     /locus_tag="IXF54_002850"
     CDS             988..1593
                     /locus_tag="IXF54_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAQ1406049.1"
                     /translation="MVLENKLGITNQIALAKAEERISKQKAKHLYDSGKINEIEVGTF
                     KGLATIHFYLFSDIYKFAGEIRTVNLAKGNFRFAPLMYLETSLQYIDKMPQSTYKEII
                     EKYVEMNIAHPFREGNGRSMRIWLDLMLKKELQKVIDWNLVDKEDYLSAMERSPINDL
                     EISHLLSQALTEQIHDRQLFMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          788 a          242 c          338 g          625 t
ORIGIN      
        1 cgcaaaaaaa tcagggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac
       61 agttaaaaca aggtttcatt gatattgtga atggtcaaac tcctcaaact cctcaaactc
      121 cagtagtaga agaatctact caacagtctc aacaaacaga aaccactact gaacaagtaa
      181 aattatatgg agaagttctt actgatccac atgaaaaggc agaaatgatt agagacctat
      241 atgagggtaa tcaaggattt aatagagata ttttgaattc aattccagat caagaaatca
      301 ttgattcatt tgtaggtaat gctacgaatg cacaacttgc acaaagtaca acgaatctat
      361 tagaaaaata ccctaatttg aaataagagt tacaaatgga cgaggctttt aggatgattc
      421 atcattctaa catggttcaa taattagagt tatacatcat caagtaataa ctgattctta
      481 aaaagcacca taagcttttt aagaatcagt tttatctata aagagggaga aagaatgtat
      541 taaatgactt tttaactaat atacataaaa acaataatag gaatgagaat tgaaagagca
      601 caagcattct tttgaatgag atatttaatt gaaaaccaat acagtactag aagtacccaa
      661 agtgaccaaa agatagttga taaaaaatga gtaagaaaag taaaatgaga taaaaaattg
      721 tcactttagg tactttggta ctaagaaagg cttgtttttg tctatttgtt tcactaaagt
      781 atataaaaca gaattaatgc aggaatcata gaaagtttgt catactttct atgattccct
      841 gttttacttg actttttgat ataatagaat agttaagaaa gtattcaaga gaatatttta
      901 gaaaatagag tttatgagtt tatgcactgt agaaagaatt tgagcgtcaa tgatttagtg
      961 atttaatata tgaatgaggt gattctcttg gtattggaaa ataaattagg aataaccaat
     1021 caaatagctc ttgcgaaggc agaggaacga ataagtaaac aaaaagcaaa acatttatat
     1081 gatagtggaa aaataaatga aatagaggtt ggaacattta agggcttagc aacgattcat
     1141 ttttatttgt tttcagatat ttataaattt gcaggagaaa ttcgaacagt gaatttagcg
     1201 aaggggaatt ttcgttttgc cccattaatg tacttagaaa cttctttaca gtatattgat
     1261 aaaatgcctc aaagtacgta caaagaaatt attgagaaat atgttgaaat gaatattgcc
     1321 catccttttc gagaaggaaa tgggcgaagt atgcggattt ggttggatct tatgttaaag
     1381 aaagaactac aaaaggtaat tgattggaat ttagtagata aggaagacta tttatctgct
     1441 atggaacgta gtccaatcaa cgatttagaa attagtcatt tactttctca agcactaaca
     1501 gaacaaatcc atgatcgtca actatttatg aaaggaattg atgtaagtta tttttatgag
     1561 gggtattcag agtatacgat tgatgatcta taaatctgtt taggtttaat gtatttctac
     1621 acaattattc ttacaataag aaaagagcta acatagagac tcatagaaag tatgaaggac
     1681 tttctatgag tctttgtttt tcaaaatagg tatataaata acatatgaat tctttttttg
     1741 ttttatgata aaaatatgta gtacctaaag tacccaaagt gaccaaaata tacttgataa
     1801 aaaatgagtg agaaaagtaa agtgaaataa aaaattgtca ctttaggtac tttggtactt
     1861 agtaaaggat tatttatttc actaaagtat ataaaacgga attaatacag gatacataga
     1921 aaatttgtca tactttctat gtgtccatat taagtaaaga ttccccgttt tcccgttaaa
     1981 tttaaaagat aaa
//
LOCUS       DACERL010000168         1947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.178, whole genome shotgun sequence.
ACCESSION   DACERL010000168 DACERL010000000
VERSION     DACERL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.178"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            179..481
                     /locus_tag="IXF54_002851"
     CDS             179..481
                     /locus_tag="IXF54_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406050.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(997..1070)
                     /locus_tag="IXF54_002852"
     tRNA            complement(997..1070)
                     /locus_tag="IXF54_002852"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1034..1036),aa:Ile,seq:cat)
     gene            complement(1625..1939)
                     /locus_tag="IXF54_002853"
     CDS             complement(1625..1939)
                     /locus_tag="IXF54_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406051.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          290 c          301 g          648 t
ORIGIN      
        1 ttataagggt ttgagcattt tttattttcg cagattttcg ttaaatgaat gaaaatggca
       61 ttcaaaatag cattcaaaaa gaacactttt taatatatat gttccatcca tattatcaat
      121 gatattccta ctttacaaaa agaacattag ttcgtatact ctttttgagg tgaacattat
      181 gttgatggaa ggaaaaacac aattatggtt taaatttgat ccttcgaata gatttgtcaa
      241 agatttttat aaggtgtggg attcagaagt tttcttttta gcaatcgaag atagcttatt
      301 aatcaatctc tattactcta ataagaacta ctttaaaatt ccggcaacaa aaaccagaat
      361 gaagaaagat gtgtattttt tgtttgatat cgtgactgat gtgccagacg cacggagcga
      421 tcatcggcgt tatgactatt tgaagtatac tttcgttgat ccagaaaggt ataaagatta
      481 aagtaggcta cctaaaaaag gtagcccgga acggatttta tcaccatact tataaaaggg
      541 atatttttta agttagtatt aagattgtgt aatatgatga tatctatatt ttatagtatc
      601 agtgctataa aatcaaaaat aagtcactaa ttaactacca ctccaattat aagtcttttt
      661 cccattattt tttacagtta tatggtatgc tttttaatgg cttcaaatat aaaagagttt
      721 aaagcgtaac acacttatgg ggaagtggtt tttggggaac gctttaaact cttctttatt
      781 attatctcac aatataaccc aaatgtcttt ccatttaaaa atcaaagtaa aacttttcaa
      841 atatacagaa gtataactat gtgaaacatc ctttcattaa tccataaaag gatacataaa
      901 aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata ctcttcaaaa
      961 aattctaaca cagaacgatt caattggcta cattaatgta ccctgtagga ctcgaaccta
     1021 cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtag tggttgttgc
     1081 cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg ttatcgcata
     1141 tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata tatgtatggt
     1201 tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta ttttggtcaa
     1261 ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca aaaaaacatt
     1321 caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt ttacaaaaag
     1381 aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag gaaaaacaca
     1441 attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct agagaaatta
     1501 attcatccta acataaaata attgttcact caaaagtttt tctagaacaa ttaaaacagc
     1561 aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa aaggcataat
     1621 tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta ataattgcag
     1681 aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata acattaaaag
     1741 aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa atagtctgtg
     1801 aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct atccctgcat
     1861 ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt attaagataa
     1921 aattactgta aaaatccaaa agctatc
//
LOCUS       DACERL010000169         1927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.179, whole genome shotgun sequence.
ACCESSION   DACERL010000169 DACERL010000000
VERSION     DACERL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.179"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            129..983
                     /locus_tag="IXF54_002854"
     CDS             129..983
                     /locus_tag="IXF54_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1406052.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            1082..1291
                     /locus_tag="IXF54_002855"
     CDS             1082..1291
                     /locus_tag="IXF54_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ1406053.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1308..1580
                     /locus_tag="IXF54_002856"
     CDS             1308..1580
                     /locus_tag="IXF54_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1406054.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            1582..>1927
                     /locus_tag="IXF54_002857"
     CDS             1582..>1927
                     /locus_tag="IXF54_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAQ1406055.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVI"
BASE COUNT          741 a          272 c          358 g          556 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacttttgag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggcttgaaaa atggaaactt ggcttcttct attattcatt
      421 tgactgataa tttagacttg atccctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacgtttgaa aatgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatcttatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcgagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttgca agaacagttt aaccctggac
      721 tagatatgat tggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaaa gaacataaag aggataattt ggttttccga aatattatca
      841 agcgaagtaa taaagtaagt acttggtcta aaaatggcat tacagaacac aaaggctatg
      901 acaaaaaagt tttgtctatg tatgagaacg tattttttga aatgattgag cgaatcattc
      961 aattagaaaa tgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacttg
     1021 tgatttgtga taggtgatga taaaataaat agtaagaaga aatagaaaga agtgagcgat
     1081 cgtgggaaat ttaggcgcac agaaagcaaa acgaaatgat acgccaatca gcgcaaaaaa
     1141 agatataatg ggggataaga cggttcgtgt tcgtgccgac ttgcaccata tcataaaaat
     1201 cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg acaaacttta aaaggagtga aaatgaaatg gcagttacgt
     1321 atgaaaaaac atttgaaatt gaaatcatta atgagttatc ggcaagtgtt tataatcggg
     1381 tattaaatta tgttttgaat catgaactag atactaaaaa tactcgttta ctagaagtga
     1441 atcttttaaa tcaattagaa gtggcacaag aagttgattt atttcaacaa ccatttgaag
     1501 aattacaagc tattcatgag tattggcggt caatgaatca atattcaaaa caaattttga
     1561 ataaagagaa agtggcttaa catggcgaat attactgact tcaccgaaaa gcaatttgaa
     1621 gatcgtttag aaaagaatgt tgaacgacta actaaaaata gactagcggt tgaatcgcca
     1681 accgcttttt tacttggtgg gcaaccaggg tcagggaaaa ccagtttgcg atcagcaatt
     1741 tttgaagaaa cacaagggaa tgttattgtc attgataatg atacctttaa acaacagcac
     1801 ccaaattttg acgaattagt gaaactctat gaaaaagacg tagtaaaaca cgttacccct
     1861 tattcgaatc gcatgacaga agcacttatt agccgtttaa gcgatcaagg ctataattta
     1921 gtgattg
//
LOCUS       DACERL010000170         1926 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.180, whole genome shotgun sequence.
ACCESSION   DACERL010000170 DACERL010000000
VERSION     DACERL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.180"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            78..281
                     /locus_tag="IXF54_002858"
     CDS             78..281
                     /locus_tag="IXF54_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1406056.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(422..907)
                     /gene="ssb"
                     /locus_tag="IXF54_002859"
     CDS             complement(422..907)
                     /gene="ssb"
                     /locus_tag="IXF54_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1406057.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFVNCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKATTESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(910..1809)
                     /locus_tag="IXF54_002860"
     CDS             complement(910..1809)
                     /locus_tag="IXF54_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1406058.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(1822..>1926)
                     /locus_tag="IXF54_002861"
     CDS             complement(1822..>1926)
                     /locus_tag="IXF54_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1406059.1"
                     /translation="EYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
BASE COUNT          507 a          370 c          330 g          719 t
ORIGIN      
        1 catcacgatc acctcgctaa aattatagca aaaacagcta aaattgagaa tttttattcg
       61 attttagata tgctataatg atcaagacag taaagtttgt gacggtggct atccgattga
      121 aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac agaaaggaga agccttttgc
      181 ctgttgaaac ggcgctagga ttaatgatta gtttcggttc tttgatcgca acaatcattt
      241 ttggaatctt agcagttgta aacaacaatc aaaaaaaata accgtcagct ttagccggtc
      301 gacgattatt tttcaaaaat aatattaagc tggtcaccgt cttaaacggt gctgttaggg
      361 gagttgttag cgcaactcct cttttcgtat ccattataac aactttattg atcttttaca
      421 atcagaatgg taaatcatca tcactaatat cgattgattg tccgccaaat ggattttgtg
      481 aagcgttcaa tccattgttg ttaggcgtag ctacatcccg ttgttcaaag gtggtagggc
      541 ttggactgct ggacgattga tcagcgtgcg cacggctctc tgtcgttgct ttactttcta
      601 atagttgaaa gttatcagct acaacttctg tcacatacac acgttgtcct tgttggttat
      661 cgtaggttcg ggtttgaatc cgtccgacaa cgccaatcaa gctgcctttt ttagctaatg
      721 tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt gacaaaatcg gcttctcgat
      781 tgccgttttg attcgtgaaa ttacgattaa cggctaaggt aaaagtggcc actgctgtgc
      841 cacttgcggt ataacgtaaa tctggatctt tggttaaccg tcctactaaa gttacattat
      901 taatcatctt tatctctcca tttcttgatt catttgttgt gatcttttaa actgcctttt
      961 tctttcttga ttttcaattt tgatcacact tttcattagg tgatcaattt cgttgactgt
     1021 atctgcaaat gaaatggaat tcgcaaacgt gttaactgag cgatattttt cccactgctt
     1081 ttccaaatag tcgtttcccc tgatcttatc catttctctt tcataataaa aggataaagg
     1141 ctttgtatct ccacgttttg taaatgtatt tcgaacactt ttgtataact ctttcgtatc
     1201 aatattttct agtttcggaa tggtaaataa gtcgtagtaa tcttttgctc tcgaattatc
     1261 tgtgccaaag ctgaacgtag cacttagctt ttctgcaata atttgttctg ttggataagc
     1321 tggaatctcc acttttttat cttcaaacat taattggtga tagtgtgtat ctacttcagg
     1381 ataaatagaa tctcctgttg tcacatccat tttaaacggt acacgtactt tctctaaatt
     1441 agccaccaat ttaatttgaa atccaggata aaaatcggct tctcttgttt ccttcattcc
     1501 ttgaatagag aaaacaatgc cttcttttgt gggttttcta gttaaatcat taaaaatgct
     1561 cttcaatttt tcttcagtta atttgctatt tctaaatgtc gtatcgatat caattgttgt
     1621 ccttctatcg attccatatt ttgatcctaa taaaaagccg cctttcaaaa tgaagtcatt
     1681 tttgtacttt gattcagaaa tttgttcaat tagacgatcc atcataaaat gccgttgcac
     1741 taactgtgca tttaatcctg tactctttgc taaatttctt gtttttgctg caaattgtgt
     1801 cggtgtcatc tttttcctcc atcaatataa gctttgcatg tggtatttca tttctttttc
     1861 tacatgtagt tttgtcatgt aatctcttaa tttacttgga tctctcaatg ggtcttccat
     1921 gtactc
//
LOCUS       DACERL010000171         1901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.181, whole genome shotgun sequence.
ACCESSION   DACERL010000171 DACERL010000000
VERSION     DACERL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.181"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1..673)
                     /locus_tag="IXF54_002862"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="IXF54_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            751..1790
                     /locus_tag="IXF54_002863"
                     /pseudo
     CDS             751..1790
                     /locus_tag="IXF54_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          553 a          403 c          398 g          547 t
ORIGIN      
        1 cgggaattct aaattcagta atatctgtta accatttttc atttggcttc tttgctttaa
       61 aattccgatt taataaattg gggtgtgcag gagaaatttc cccagcatac gaagaatact
      121 tttttcgtct agaaaattta acaaatagat tttcctcagt catcaatcta cgtacaactt
      181 tttcagaaag ctttttaccc atatttttta aagcttggtg aattctacgg taaccatacg
      241 cacagaagtt atgattgaaa atcgtgataa ttaattgacg ttctttgtaa tacttatctg
      301 gtttctcaag tgcttgtttt tgataaaaat agctgctttt aggcaactgg agctgttgaa
      361 gaagctcttt caaggtgaac tgatttcgta aggcatcaat caatatcgtt ttttcttgat
      421 ttgatagaca aagaagattg acgccttttt ctttttttag cagttcagcc gctccttcga
      481 gtaatgcttt ttcgagctgt aattggtgta cttgtttttg taagtcttct acttccttta
      541 aaagtagctc tttttgtttt tcctgatttt ttaaagtcat acgcaatgga aaatcctttc
      601 caagaaccgt gtttttccat tgatatagag taactctact aattccttca gattcggcta
      661 aatttttcct attatataaa ttgagttaaa ttgtattcgt ttttcctcct ataggcgtat
      721 actaaagaaa acacttgagg agtggggaac atggagacag aacgccaaat ccgtgaattg
      781 gaaaaagcga tgaatacgac agacaaccgc cggttgtacg agcgtttcct ggcagtcaag
      841 ttggttcttg aaggacacac ctggaaagaa gccgggcaca ccatcggccg cgacgagcat
      901 actgtcagcc actatgtgaa aaactactgc cataatggaa tccaaggttt agtctccaaa
      961 aaacaaagcg gacgaccttc acggctgaca gatgaacagg aagcggaact ggtccaaata
     1021 gtcgcctacc acactccgga ggaagtaggt ttcaaatccc gtgccaattg gacattggcg
     1081 attgtgtgtg acctgattct ccgggaatgg agttatggat atacgcaaaa aggcgtggcg
     1141 gacctccttc accggctagg actaagctgg acccgtccga cgtataccct caaaaaagcg
     1201 gattccaaga aacaacaggt atttttggaa gaaaccttcc cgagcctaaa aaaaaaattg
     1261 ctgaatagcg atattgacca cctcctattc gaggacgaaa ccatgatccg ggattaccaa
     1321 gccattggct gcacctggtt tctccgcggc caccagcgaa aaatccccac ttatggacaa
     1381 cacaaagggg taaaattgat aggcaccttg gattatgaag ccggtcggat catttgtaca
     1441 gaagaagggg catacgacgc agcgaccttc ctccgttttc tggagcggac gttgcgggaa
     1501 taccccaccg gaaaaatcgt gatggtattg gataatgccc ttatccacca cgctaagctg
     1561 atccagtctt ttcttgaaga aaacgctagg cgcctggcac tcatcttcct ccctccctac
     1621 agccctcagt tgaacctaat ggaaggcgtt tggaagtggc tcaaggaatc ggtcatcaac
     1681 aatgtttttt tgaccacgtc cagaaaatca aacagtcggt ccgcggtttc ttagccgatg
     1741 taaacgagcg tccccttgag gtgattgacc gactgtgcgt gcggatgtaa agtatttaat
     1801 tcaacctata tagttaattg tagcctatcc aaatcggaat tttaaagcaa agaagccaaa
     1861 tgaaaaatgg ttaacagata ttactgaatt tagaattccc g
//
LOCUS       DACERL010000172         1891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.182, whole genome shotgun sequence.
ACCESSION   DACERL010000172 DACERL010000000
VERSION     DACERL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.182"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            21..623
                     /locus_tag="IXF54_002864"
     CDS             21..623
                     /locus_tag="IXF54_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406060.1"
                     /translation="MNIERLKRMYDGYDGDRIIEVRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGREISLKDESNTTVSDANILANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAA
                     LCRIHHTEKHKIGVTNFKNKYQIKGIKLNQETIKKLRIGG"
     gene            626..853
                     /locus_tag="IXF54_002865"
     CDS             626..853
                     /locus_tag="IXF54_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406061.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            856..1683
                     /locus_tag="IXF54_002866"
     CDS             856..1683
                     /locus_tag="IXF54_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HAQ1406062.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1695..>1891
                     /locus_tag="IXF54_002867"
     CDS             1695..>1891
                     /locus_tag="IXF54_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406063.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLA"
BASE COUNT          705 a          305 c          399 g          482 t
ORIGIN      
        1 gtgcaactat taatgaagaa gtgaacatcg aacgtttgaa gaggatgtac gacggatatg
       61 atggtgatcg gattattgaa gttcgattta ttgatccacg tcggttcact gtgcagcaac
      121 gaaacttcat ctatgcactc ataggcgata ttttcatcga tacaggcatg ccaacggact
      181 tctggaagga attcttttac ttccgtttcg aaggtgtcac agggcgcgaa ataagcctca
      241 aagatgaatc gaatacgact gtgagtgatg ccaatatctt agcaaatatc atcttagatt
      301 ttatctttga acatcatatt cctttcaaag aaggctatga gattttacca gcgaatcaag
      361 aatattactt ctacaaatgc atcacaaaaa gagtctgctg tatctgtggc aaaacaggag
      421 ctgatatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatt
      481 cagagtacac atttgcagca ctctgtagaa ttcatcacac ggagaaacac aaaataggtg
      541 tgaccaattt caaaaataag tatcaaatca aagggattaa gttaaaccag gagacaatca
      601 agaaattaag aatcgggggc taatgatgat taatttaaac gcagtagcgt ttgaacaaac
      661 atggcgcact aagtataaaa aaatgagtcc aagagacaaa ttgttcttag aaatcatgac
      721 atttgctttc attggtacac aagctgaaca aagcgatatt agtgttgaaa aaattaagac
      781 gaatagatta gttaatggaa ttacagagac ttgttaccag tacacgatta tcgtcgtgga
      841 tgaggaggaa taattttggc agaaagaaga atgttcgcaa agacaattat tgattctgat
      901 gcattcctag atatgccttt atcgacacag gcattatatt tccatctatc aatgcgcgca
      961 gatgatgatg gatttattaa taatcccaaa aaaatacaac gtatggttgg ttgtggtgat
     1021 gatgatttaa aactgcttat ggcaaaaaga ttcatcttag ttttcgagag cggtgtgatc
     1081 gtcattaagc attggaaaat acacaactat attcgtaatg atcgttacaa acccacgtta
     1141 tatcaggatg aaaaagcttt actagcagac aaagataaca aagcttatac atttgcagaa
     1201 gagctttcta aacatgatga aaaacttggt ataccagatg acaaccaagc ggtataccaa
     1261 atggatacac aggttaggtt aggtaaggat aggttaggta aggatagtaa agagataaaa
     1321 gatttaacgc cttcgaaaaa atcgaaggct aagcccatcc gtcataaata cggagagtat
     1381 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt ccctaatgat
     1441 taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa
     1501 aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca
     1561 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa
     1621 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
     1681 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
     1741 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
     1801 ttgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
     1861 atatcgatac aacacaatta agaaatttag c
//
LOCUS       DACERL010000173         1845 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.183, whole genome shotgun sequence.
ACCESSION   DACERL010000173 DACERL010000000
VERSION     DACERL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.183"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(245..1108)
                     /locus_tag="IXF54_002868"
     CDS             complement(245..1108)
                     /locus_tag="IXF54_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ1406064.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(1110..1382)
                     /locus_tag="IXF54_002869"
     CDS             complement(1110..1382)
                     /locus_tag="IXF54_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1406065.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(1399..1614)
                     /locus_tag="IXF54_002870"
     CDS             complement(1399..1614)
                     /locus_tag="IXF54_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ1406066.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(1706..>1845)
                     /locus_tag="IXF54_002871"
     CDS             complement(1706..>1845)
                     /locus_tag="IXF54_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ1406067.1"
                     /translation="SNKVSTWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEK
                     E"
BASE COUNT          494 a          331 c          289 g          731 t
ORIGIN      
        1 taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca
       61 cctctaataa ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt
      121 ttaaatctat ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga
      181 aatgttttta taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta
      241 aggtttaaat acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc
      301 tttccaattt ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca
      361 ttttttgctc tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca
      421 attctttttc taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt
      481 ttactccttc tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt
      541 ctaaattggt cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg
      601 ctgtcattgg atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt
      661 tgattttagg tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg
      721 ttgcggtttg aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc
      781 cttgatcgct caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt
      841 gttttactac gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt
      901 taaaggtatc attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc
      961 gcaaactggt tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa
     1021 ccgctttttt tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt
     1081 tgtcagtaaa attgactata tttgccatat taagccactt tctctttatt caaaacttgt
     1141 tttgagtaac gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa
     1201 taatcaaaaa gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat
     1261 aattgagagt catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa
     1321 acgcttgccg ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc
     1381 atttcatttt cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa
     1441 ggcttgatcc ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat
     1501 atggtgcaag tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact
     1561 gattggtgta tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt
     1621 ctttctattt cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt
     1681 gattaatcac ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct
     1741 caagcatttc aaaaaatacg ttcttataca tggataaaac ttttttgtca tagcctttat
     1801 gctctgtaat gccgttttta gaccaggtac ttactttatt acttc
//
LOCUS       DACERL010000174         1801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.184, whole genome shotgun sequence.
ACCESSION   DACERL010000174 DACERL010000000
VERSION     DACERL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.184"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..270
                     /locus_tag="IXF54_002872"
     CDS             <1..270
                     /locus_tag="IXF54_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406068.1"
                     /translation="TVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQQLEELLN
                     RTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            270..>1801
                     /locus_tag="IXF54_002873"
     CDS             270..>1801
                     /locus_tag="IXF54_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1406069.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYR"
BASE COUNT          653 a          295 c          348 g          505 t
ORIGIN      
        1 acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt agccacaacg
       61 gcgtcaatca aggaagaagc ccaggaacaa gcaagaaaag agtatgaaca acaattagaa
      121 gaattattga atcgtaccaa tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag
      181 agtgattcta gtaaggctaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc
      241 gaaagttcag aatcaaatga ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag
      301 acaaacgatt caatttagaa aagaataggt atctttctca tttctatcgc tatactccct
      361 caggagaaga gctaccagaa aaacatgaca aacgcatttc gaaaaagatt ttttatagtg
      421 gacttgtata cctcccttta gagtatttag taacaagagt accagaagtt ttcaaccgat
      481 tggaaattgt taagggaagc ttacaaggaa taaatctgtt tcaattacag gaaatgccac
      541 aggtctttca ttttgattca gctggttttt ggaatccatt tactcctaca actagtttgg
      601 gtacacgttt agcaatttcg acagtttgtg tggttccagc tgtacttatg ggattgaaaa
      661 tggacatgtg gtttcgtcct ctagcagatg gacaaaaagg ggataatcga ttaatgacct
      721 taaaagaaat ccaacagatt tatccgcaaa tacctgaaac aaaaactact tttccaggtt
      781 atggaggtat tccaatcaca cattacaaaa aatattggta cattcaaaca gatactgtta
      841 atacgtgtat tgttggtacc tctcgatcag ggaaaggtca gattgaagtg ttagccacca
      901 ttgacaattt atctcgtgca gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag
      961 aattatatgt tggtagtaaa gatgaactag aagctagagg gtatgatgta tatgccttta
     1021 atatcaccga tccactgcaa tcaatgagtg ataatccatt ggcactcatc gtgaaatact
     1081 ggaaacgagg agacatagat actgcaacac agttaaccaa tactttcaca aatacgattt
     1141 attatgatgc caacgcttca gataacaaat actttaacga aaacgcgcag aaagctgtca
     1201 atgcgcttat ttttgcgttg ttagaacaag ctgatcaaac aggagattac tccaaattga
     1261 caccgaataa tctcgttgag cttctttctg agattggcgg ttttaattat tatccagatc
     1321 cagacaatga attcaaacag ttgaatgcct tagatgaatt tatgaatcag ttgccacctg
     1381 gaaacattgc aaaaaaacaa tatggggcaa cgaaaattgg atctgataaa gcgaagggaa
     1441 atattttatc aacggcaatc acgggattga atccatttac tctaacgaaa attgcaaaga
     1501 tgacttctca atcaacgatt gattataaat cggttgggtt tccaaaatac ttagatttga
     1561 aattacatga aagtctttta aatcaacgag tcgtagtaga attttatcat aagggaaaga
     1621 aatttcatga agaacgtgtc aaagtaggct ataaaggatt ttgtgaaatc aattttgatt
     1681 gtcatttagt tgcaggtgat caagtttgtt taaaatacga tcatcaagga aaagaatatg
     1741 tggcttggta tgaattttct caaagagtat tgaaagacga agagggtaat atcgtttatc
     1801 g
//
LOCUS       DACERL010000175         1770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.185, whole genome shotgun sequence.
ACCESSION   DACERL010000175 DACERL010000000
VERSION     DACERL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.185"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            197..424
                     /locus_tag="IXF54_002874"
     CDS             197..424
                     /locus_tag="IXF54_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1406070.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IXF54_002875"
     CDS             547..873
                     /locus_tag="IXF54_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1406071.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IXF54_002876"
     CDS             941..1654
                     /locus_tag="IXF54_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1406072.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          373 g          438 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt
//
LOCUS       DACERL010000176         1764 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.186, whole genome shotgun sequence.
ACCESSION   DACERL010000176 DACERL010000000
VERSION     DACERL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.186"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            131..505
                     /locus_tag="IXF54_002877"
     CDS             131..505
                     /locus_tag="IXF54_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1406073.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXF54_002878"
     CDS             complement(619..1248)
                     /locus_tag="IXF54_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1406074.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXF54_002879"
     tRNA            complement(1382..1454)
                     /locus_tag="IXF54_002879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IXF54_002880"
     tRNA            1668..1741
                     /locus_tag="IXF54_002880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          613 a          258 c          309 g          584 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaa
//
LOCUS       DACERL010000177         1761 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.187, whole genome shotgun sequence.
ACCESSION   DACERL010000177 DACERL010000000
VERSION     DACERL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.187"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..400
                     /locus_tag="IXF54_002881"
     CDS             <1..400
                     /locus_tag="IXF54_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406075.1"
                     /translation="EALKRHYHRLFKRPISQTEIVNELLSYNEELRTAYHFCQLLRYY
                     FVKRDTVGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLYNKI
                     KLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            579..>1761
                     /locus_tag="IXF54_002882"
     CDS             579..>1761
                     /locus_tag="IXF54_002882"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1406076.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          555 a          355 c          320 g          531 t
ORIGIN      
        1 ggaggcactc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
       61 cgttaatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt
      121 attacgctat tatttcgtta aacgagatac ggtaggcttt caagaaaccc ttaaaacaat
      181 ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatc gctaccaatc
      241 gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgtataa
      301 caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc
      361 acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga
      421 acgcgccgca tatcggacta agttttattg gctctataat ttctaggact gatggaaatt
      481 ttctatctgt cctagttttt ttgtaaaaaa acaaaaagac atacaaaatt tccggtagaa
      541 tagagtctcc tacaacatca ttctggggga attcttgtat gtccatgaat cattttatca
      601 aacaactctt tactattaaa gactcaaata tccaaattca atcctttgaa gaaacttttt
      661 ataaaagcag aaaaagtatc gttattgagg gaactctgat ccgtacctac agacgttgtc
      721 cgtgttgtaa agattctaca aagcaaatcg ttaaaaatgg caagaagatc tctatgattc
      781 tcctgaatcg tagcggtaat aaacggacct atcttcggtt gaagaaacag cgatacctct
      841 gccgagcatg taaaaaatat tttaccgctc ggacgtatct cgttacccca ttctgcttta
      901 tttctaagca gattcattac aagatcttag aagaattgac agagcgtcaa tctataaaag
      961 caattggtaa acattgcgat gtttctgtga caactgttca gcggacattg tccgccttgt
     1021 catcagatat ccgtcagaat cttgagtatt tacctcggtg tctattattc gatgagtttc
     1081 gttctctttc tacatggcac ggtaaatttt ctttttcttg tatggacggt gagaccggga
     1141 aactgtttga gatcttacct agccgccgta aaaaagatct cgttgctttt ttcatgcgtt
     1201 tttctctcaa agcgaggttg ggtgtacatt acatcgtaac ggatatgaac gctccttatt
     1261 tctcactagt taaagagtgt tttccaaatg cacaagtgat cgtcgatcgg tttcacattg
     1321 ttcaacatct gaaccgcttc ttcgatgcgg ttcgaaaacg tgtgatgaaa acacttgatc
     1381 aaaaagaccc aatacaagct aaacagtatc gccaattgaa atccttattc aaattacttt
     1441 taaaatcaga ggatcgttta gattacaata cactaacgaa aagacgcaat ttcaattgga
     1501 gactattgac tgaaacagaa gtagttgacc gccttctaaa gatttctcct gaacttaaaa
     1561 cagcttatcg ttactaccaa gacctgctta ctgcgtatca ggaaaaagat ccagatactt
     1621 ttttccagct gcttcgagca atgcctggag aagttccatt ggaactgcac catatcaaaa
     1681 aagccttttt caaatacgag aaagggattc ggctggcatt actgaagaag tattcgaatg
     1741 cacgactgga aaatctccat a
//
LOCUS       DACERL010000178         1733 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.188, whole genome shotgun sequence.
ACCESSION   DACERL010000178 DACERL010000000
VERSION     DACERL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.188"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            429..666
                     /locus_tag="IXF54_002883"
                     /pseudo
     CDS             429..666
                     /locus_tag="IXF54_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(810..1586)
                     /locus_tag="IXF54_002884"
     CDS             complement(810..1586)
                     /locus_tag="IXF54_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1406077.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT          554 a          297 c          272 g          610 t
ORIGIN      
        1 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggaa
       61 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaaggtcagc tgattgaaca
      121 ccgtttaaga aagaggtcgt gagcctgtca tttaactctg agtcacaagt aacaatttca
      181 aaaaacagct tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga
      241 atcatctttt tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt
      301 gcccttaatg aggacagtcg gaacaggttt gtcattttta tatttaaatg gcacaaattt
      361 ttctaattat ttgtcattta gcgctgaaaa tttttgctat ttatctaaaa aagtgataac
      421 tacttaagat gaagcagaat atttacgcca tacggcatac aaccgcgaaa aatataaaaa
      481 aaagaaaacg attgaacgcg tttttgcagt cgcaaaggaa aagcatggaa tgcgctacat
      541 caagtatcga gacttggaaa acaatcaggt gtatacgctg attgtttttg ctgcaatgaa
      601 tctgaaaaag ttgtccactt ggcagttgga acatatgtct tcatatcttt tattatacaa
      661 aaatagcaaa agcaccagtc tacactctta gtaaagagga aactgatcct tttgtctaca
      721 ttcttaaagt acttaaacta agtactttat tttactcttt aggattgata cagtagcatc
      781 tatcccttta ttaataatct tttatttttt taaataaaga tgttgtctcc atcttcaggc
      841 actaaaactt tttcggaaat tcctttttct ttattgaaag actttaattc ttctcttgac
      901 agtccccaat ggtttacagc ttccatatga acggcaataa ttgtggaatc tggtattgct
      961 ttatgcaatt catataaatc gtctttcccc ataataagag attctccaac taaaaaatga
     1021 ttatctccag catttgctac tatgatttct ggattatact gatcaatagt ttgtttaaca
     1081 gcttcatacc aaactgtatc tcctgtaaag tatagtgtct tctcagtcga atgtttgaaa
     1141 acaataccgc aaacattacc tacataattc aagatttctc ctctgccatg tttgcctttt
     1201 gttttaacca attgtataga ttcaaaaact gtgtcttctc gtagtacctc cacattttta
     1261 aaaccatata gtcgaatttc attcgcatca ttttcatctt gcacaaataa ttttatttct
     1321 ttaggtagga cctctttcgc tatatcgtcc caatgatcta gatgtaaatg tgttacaata
     1381 acagcatcaa taccgttgat aatatcttcg actggcattg gcaaatcaac tagaggattc
     1441 atttgatctt ttcttggtga tggaaaagga cctttcgaaa taaatggtgg aagtgttcct
     1501 ttttccgcta acattggatc aattaaaaat tttttacctc catagtctaa gatagctgtt
     1561 gcattacgaa tgtgatgtat attcataaac gttgtctcct ttttgttgat atgaaatatt
     1621 tttagtataa ttcaattcca tacaataaat aaaccgaaca aaaaaagtct tttactattt
     1681 ttacttaatt tatgaaaggt tgtttaccat gttagaccaa acagatataa taa
//
LOCUS       DACERL010000179         1689 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.189, whole genome shotgun sequence.
ACCESSION   DACERL010000179 DACERL010000000
VERSION     DACERL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.189"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            5..>1689
                     /locus_tag="IXF54_002885"
     CDS             5..>1689
                     /locus_tag="IXF54_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406078.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEE"
BASE COUNT          691 a          278 c          371 g          349 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaatga acaaatcgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggttataaca agtacggcgg
      301 actagatatg acgatcgaaa atattccaag aatcagagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg tctttttcag ccgccacgat ccgaaaatat caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag taacgcaatg gaaaaaaaat ccgaccattt attttctgaa
      541 aggctatcga aaagtcgctt tagaactgaa tgaaaacggg gaatttgagg aatctaaaaa
      601 ataccaagcc acatcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctctgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggagag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg acaaccaata cgctgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggatatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatca ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaactca gttaaaacct gaagactatc cgaaaatctt
     1501 cccgaaccgt gtctttgatt ttcaattgga tcaaacaggg cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atatcgggat tcaaattcga gatattacgg agagtgaaat
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaat
//
LOCUS       DACERL010000180         1669 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.190, whole genome shotgun sequence.
ACCESSION   DACERL010000180 DACERL010000000
VERSION     DACERL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.190"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..338)
                     /locus_tag="IXF54_002886"
     CDS             complement(<1..338)
                     /locus_tag="IXF54_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1406079.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGP"
     gene            complement(452..664)
                     /locus_tag="IXF54_002887"
     CDS             complement(452..664)
                     /locus_tag="IXF54_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406080.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            851..1318
                     /locus_tag="IXF54_002888"
     CDS             851..1318
                     /locus_tag="IXF54_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406081.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            1315..1557
                     /locus_tag="IXF54_002889"
     CDS             1315..1557
                     /locus_tag="IXF54_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406082.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
BASE COUNT          582 a          244 c          297 g          546 t
ORIGIN      
        1 ggcccttcat aagtccagcc gtaattttta gtcaaccatt tcacttcatc ccaactagct
       61 gtgtaatggt gatcacgaag acctttattg tacaaacgat agacaagatg gccaccgcta
      121 gtcggtgctt cccaggcggg gccttcataa tttccccatt ttatctttac tagatggtca
      181 cgttcttttt tgctagaggt gtaaaaatgt tcatgattat ttggattgta aaggcgatac
      241 atgacgttag ttgaagcagc gtcaacagat ccagcaagac taagtgaggc agcaaatgtt
      301 ccaatagcta aaacagtttt tggattaaat aatttcattt tttcaactcc taaatttaat
      361 taaaaatgtt tacatgatta ttatatatcg gctaaccgat atagtcaata aaaaataaga
      421 aaagccttat ataattttgg cttttcttat tttatttgtt agttgaagta ttaatatcaa
      481 cgatttcttc agccaattta tttaatttca tagcatttat gaatctcatg ttgtctatac
      541 tagaacgttt gttggcaagg tcacttactg tagttaaggg tacgccagat tctatggata
      601 tagaacgaac tgatttacca ctatcaaaca gcaaccattc gattttcttt ttatcagcgt
      661 acatttcaat gctccttctt gttttttctt tattatagct cttttttgta ataaatcaca
      721 atatcaagag aaaaattttc tatagaaagg tggattttta tgagtattcg ttctccgtgt
      781 atccaaatga ggtttgttgc tactttcgtt atttgatata ctaaaaataa acaacgaaag
      841 aaggaataaa atgaagaaaa agcaactatg gtttgtctct atgtttgcaa tattttttat
      901 tattttaagt ggatgttcaa gtaataagaa atcggaagct aaagaagata atagtttatt
      961 gcaaccagga actgtttgga aagaagaagt aggtggtctt attcataatt taaaaattat
     1021 agatgatact acctgggaat attctgaaag tgtatggcat ccagatcctg tacaaataac
     1081 agttaagagg caaaaagact atgaaggttt agaaagatac aaaatagtag attctgcagg
     1141 cattagggaa tttataaaca aacctgattc attatttatt gttgttccct atgagaagga
     1201 tggcgtaaaa aaaataattt ttcttggatc tagcaaggat gagaagcgaa cgaaagagaa
     1261 attaattcat gatggatcag agttaaataa atacaaatta caaaagacta gtgagtgatt
     1321 gctagtcttt ttatttatcc tgaaggagga atcacagtgg caaaagttaa aacaccattc
     1381 gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt ctgaaataca gtggaaaaaa
     1441 gaagttcttg aaaaagcaca atttgaccta ttacgaggaa aagataagga cttagaagaa
     1501 tatatctatc aacgtgaacg tgaaaaactt gtattcgatg aaattattaa acattaaaag
     1561 gagcgaataa tcttatgttt gaatcaatta caatgaattt tttaactcaa ccgaaagtag
     1621 tagctgcagt tgatattttt agagcattac aaactgccgc aactagttt
//
LOCUS       DACERL010000181         1645 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.191, whole genome shotgun sequence.
ACCESSION   DACERL010000181 DACERL010000000
VERSION     DACERL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.191"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..500
                     /locus_tag="IXF54_002890"
     CDS             <1..500
                     /locus_tag="IXF54_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1406083.1"
                     /translation="VAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAFHYVADYE
                     NLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAM
                     FLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMMLIVS
                     AKKKM"
     gene            533..1441
                     /locus_tag="IXF54_002891"
     CDS             533..1441
                     /locus_tag="IXF54_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1406084.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1438..>1645
                     /locus_tag="IXF54_002892"
     CDS             1438..>1645
                     /locus_tag="IXF54_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406085.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDN"
BASE COUNT          585 a          234 c          370 g          456 t
ORIGIN      
        1 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
       61 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
      121 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
      181 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
      241 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
      301 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
      361 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
      421 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
      481 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
      541 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
      601 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
      661 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
      721 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
      781 agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt
      841 attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat
      901 tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag
      961 aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta
     1021 tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat
     1081 tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa
     1141 attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga
     1201 gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct
     1261 atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta
     1321 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
     1381 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
     1441 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
     1501 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     1561 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     1621 gattgccttg aatatattga caata
//
LOCUS       DACERL010000182         1609 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.192, whole genome shotgun sequence.
ACCESSION   DACERL010000182 DACERL010000000
VERSION     DACERL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.192"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            287..694
                     /locus_tag="IXF54_002893"
     CDS             287..694
                     /locus_tag="IXF54_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406086.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXF54_002894"
     CDS             708..1109
                     /locus_tag="IXF54_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406087.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXF54_002895"
     CDS             1111..1482
                     /locus_tag="IXF54_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406088.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          600 a          206 c          282 g          521 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacga
//
LOCUS       DACERL010000183         1606 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.193, whole genome shotgun sequence.
ACCESSION   DACERL010000183 DACERL010000000
VERSION     DACERL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.193"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1..81)
                     /locus_tag="IXF54_002896"
                     /pseudo
     CDS             complement(1..81)
                     /locus_tag="IXF54_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            430..1515
                     /gene="xerS"
                     /locus_tag="IXF54_002897"
     CDS             430..1515
                     /gene="xerS"
                     /locus_tag="IXF54_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1406089.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          611 a          275 c          218 g          502 t
ORIGIN      
        1 taaaaaaatt gtatctccag gatgcaaccc attttcttta agaaattcct tttaaattgt
       61 taatactact gagtgcccca cttctctgaa ttttctttaa cttctccctc ccctttttta
      121 tttgtatata cattcatata cgcttgttaa tatcaaatca accgtagtct ctcttatgtc
      181 taattatata gaaagattgt tctttctata taatttagac aatttaaaaa attaagattg
      241 cctccttctg tttcctttcc tattcatgta taccatttat tttaaattat taaatctata
      301 cctatttatc tgcttttttg aaataaatta ataaatttca aagtatacca attgacaaaa
      361 cactctataa ttggtatagt ccaattatag agatactcct ctcccctact tatcgaaagg
      421 atgaataaca tgaaaaatac agaaatatat caaaaaatag atacaatcat acttcatatg
      481 cccgattata taaaagattt tgttcaagat cgtgaggata aagatcaatc acctcgaact
      541 actttagaat atctaaaaaa ttataagctc ttttatgaat ggcttctgag tgagtctatc
      601 gttcccgata cttcaatcac ctcgataaga cagttaactg cctatgatct aaacttgtac
      661 aaatctcatt tgaaacgtcg cacaaaggaa aatgtaaaaa aagagacaac aaaaactaag
      721 ctcaaagata ataataactt aggtctatct acttctacta tcaatagaaa tatcacagca
      781 ttaaaggttc tatttaaata tctttcgaaa agttccaata atcctttagg aaaaccatac
      841 ttagaaaata atcctatgga tcaagtagca actatcacag ataaaactac gttagctgcc
      901 cgtgcaaacg caatcgagaa aaaattattt ctagacgaag atacacaaaa ttatctagat
      961 tatattgcca atgaatataa aaaaactttg agcaaacgag ctttgatcta ttatcaacga
     1021 gatgtagaaa gagacctggc gatcaatgca ttgattttag gatctggatt aagactctcc
     1081 gaggttgtaa atattaattt agaggaccta tctttagaaa agaataatgt ggtagtaact
     1141 agaaaaggaa ataagagaga cgcagtaaat atagcagcat ttgcaatgga atatttagct
     1201 aattatctag ctattcggaa agaacgatac aaagtaacag aaaacgaaaa agctttattt
     1261 ctagcaattt atcaaggaga agccaagcgt atatccggta ttgcaatcga acggatggta
     1321 gcaaaatatt caaaaggatt cagagtccaa gtcagtccac ataaattacg tcatactttg
     1381 gctactagac tctatcaaca gacaaattca ttggtattaa ctgcccaaca attgggacat
     1441 acaagtacaa atacaacaac tttatatact catatagaca atgcagctac aattgatgct
     1501 ttgaacgctt tgtaaatgta gtaaaaaata gtataataat gaagcctgtc tctaaaatta
     1561 gataaaagcc caactttaaa ggcaagcttc attatcattt ttatat
//
LOCUS       DACERL010000184         1583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.194, whole genome shotgun sequence.
ACCESSION   DACERL010000184 DACERL010000000
VERSION     DACERL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.194"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            21..623
                     /locus_tag="IXF54_002898"
     CDS             21..623
                     /locus_tag="IXF54_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406090.1"
                     /translation="MNIDRLKTMYDGYDGDRVIEIRFIDPRRFTVQQRNFIYALIGDI
                     FIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPF
                     KEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAA
                     LCRIHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            626..1375
                     /locus_tag="IXF54_002899"
     CDS             626..1375
                     /locus_tag="IXF54_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1406091.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPIRHKYGEYKNVLLSDEQME
                     KLKTEFPNDHQERIERLSEYCESSGKTYKNYLATIRSWAKKEKSEPKNTSSGYKRTGR
                     REKLPEWAIDQEAYQKKKALERANRQSKAPF"
     gene            1387..>1583
                     /locus_tag="IXF54_002900"
     CDS             1387..>1583
                     /locus_tag="IXF54_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406092.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLA"
BASE COUNT          613 a          271 c          316 g          383 t
ORIGIN      
        1 gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
       61 atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc gtacagcaac
      121 gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg ccaacggact
      181 tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa ataagcctga
      241 aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc atcttagatt
      301 tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct gcgaatcaag
      361 aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc aaaacaggag
      421 ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatg
      481 cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg
      541 tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca
      601 aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg cgattatacc
      661 agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat
      721 aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt
      781 atatggtgtg aataaagaga ccatatcacg atgggtaagt gatttgatta agtttggata
      841 cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg
      901 aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga
      961 taataataca tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc
     1021 gccttcgaaa aaatcgaagg ctaagcccat ccgtcataaa tacggagagt ataaaaatgt
     1081 tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg accaccaaga
     1141 gcgaatcgaa cgactgtcag agtattgtga atcatctggt aaaacttata aaaactattt
     1201 ggcaactatt cgaagctggg caaaaaaaga aaaaagtgaa cctaagaaca caagtagcgg
     1261 atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta
     1321 tcaaaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat tctaagaggt
     1381 ggaaaattga aaatcgatta tctagaactg atcaatgaaa tagcaagtta caaaactggt
     1441 gaggaaatag agattctgag agacgtttat gaacaacttg atgaagctgg aatcgaacga
     1501 attaagaatg atcgttcaag ttggagtaaa ctcagatact atttcgcact ttatatcgat
     1561 gcaacacaat taagaaattt agc
//
LOCUS       DACERL010000185         1569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.196, whole genome shotgun sequence.
ACCESSION   DACERL010000185 DACERL010000000
VERSION     DACERL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.196"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..609)
                     /locus_tag="IXF54_002901"
     CDS             complement(<1..609)
                     /locus_tag="IXF54_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406093.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPW"
     gene            650..1342
                     /locus_tag="IXF54_002902"
     CDS             650..1342
                     /locus_tag="IXF54_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1406094.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          475 a          295 c          286 g          513 t
ORIGIN      
        1 ccaaggatta aatgaaaacg tacttggcac gacgtgatct ggatcatagg tctcgatgat
       61 tttttcgtat tgttccttgt agtcattatc aacttttgct tgggggatca ctttacttgc
      121 ttcatgcaaa aagtgttctg aaccacgccc cattgcctgt ccccgaacag tatgtttatc
      181 caacgcaata tcaggaattt ctggaatata tcccatagcg aaactcttga taacaatgtt
      241 cttaagtaaa tccgatgaac gatctttttc agattggcat aggtaacgaa tcgcgtggaa
      301 aaaatacatc gcacgatcgg attcattgta ttggaattct ttgcgcatgg cgtttaagtt
      361 attgatcatg atggcggcca taggattgcc catgcccaca tcctcaacac tgatcgccaa
      421 tagccttctc cagagtttct cttcaaattg tggtgaggtg atatacattt cataagcaaa
      481 ttcacatgct tctttttctt ttcctcgacg aatcgatttc tgtaatgctg agatagcttc
      541 atctccacgt aagccgttac gggtagtcat ttttgaccac ggatcttgaa taaatttcgt
      601 ttctgtcata atataatact cctattagat aaaatttgag gtgaatctta tggatgttga
      661 aatgcttata gataaatacc aactgaatga ctcagaagct gaagtcttac gatttatgaa
      721 aaaaaatcgt cagtctttaa aaaatattgg gattcgtgac gttgccaaag catcgtatgt
      781 ttctactgca acgattatca atatggcgaa gaaaattgga tattccggtt atagtgagtt
      841 ggtatttgct ctaggaaaca gccaatctga cattacgatg ccaagcacat tctccaatga
      901 ggagaaggaa gcattcatac atttaatgtc tgcgtatcgc gataaacgga ttatgattct
      961 tggctcaggt ttttcgcaaa atctagccaa ttatttttcg gaatctctta atctatatgg
     1021 atttcgtgcg acggccaata gtcatttaga atttcttaga cagcaagtgc aacaagatat
     1081 tctgcttttt attatttcca attctggtga tacagttcga ttagccgaat tagctaaaat
     1141 tgcgaacgat cactttatcg atgtgatcgc atttgttggg caaaaaaatt caaaaatcgg
     1201 tacgctggca acattaacca ttagcacgga aacctatatt ccacaaaaaa tatctgatta
     1261 ccaaccaaat cttttctttg gcacgacact gaatcaattt gagcttttgt tgagcgcggc
     1321 gctgaaggct atttataaat agaagaacgc tgtggccact gtttcttcaa aatctagtat
     1381 aaagaatctt ctttagaatg taattgtttt ctgtgtgttc tgaacatgtt taggaatttc
     1441 caaacttgtt tagaaaactt tctcattccc tccgctgtag aattgaactc acataaaaaa
     1501 catctaaacc aaaatatcta caagcttgga aaggataata ggttgttatt aaaaataatt
     1561 tttattcct
//
LOCUS       DACERL010000186         1481 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.197, whole genome shotgun sequence.
ACCESSION   DACERL010000186 DACERL010000000
VERSION     DACERL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.197"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..276)
                     /locus_tag="IXF54_002903"
     CDS             complement(<1..276)
                     /locus_tag="IXF54_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406095.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
     gene            complement(822..>1481)
                     /gene="tnpB"
                     /locus_tag="IXF54_002904"
     CDS             complement(822..>1481)
                     /gene="tnpB"
                     /locus_tag="IXF54_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1406096.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          449 a          260 c          209 g          563 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      781 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      841 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      901 gtatgacaaa tagggcaagt ccattttcga atttcgagag atttcttgcc gtctttatgt
      961 ccacaattcg aacagatttg gctagatgga aaccacttat ctactttgat gattttccga
     1021 ccgtaccagt cagccttgta ttgtaattta gacacaaaac tagaccaaga aacatccgaa
     1081 atactttttg ctagtttatg attacgcaac atgccttttg tgtttaagtc ttcaatacag
     1141 atgatatcgt gatttttgat aatttctgta ctcaacttat tcagaaaatc agtacgttga
     1201 ttcattactt tttcatgcaa tcgtgctact ttacgttttt gtgtttgata atttctagct
     1261 tcaaacagat tgataccttt ttgtttagct aataaggcac gtcttgacaa cttacgttgt
     1321 tcacgtttga gtttcttttc cattttggac gtaaatttat tattatcaaa tttttgaccg
     1381 gtagaaagaa ttgcaaagtc tataatacct aggtcaatac caacagcaga attagattta
     1441 ggtagttcgc gaacttcttc tttgcataac aaagaaatat a
//
LOCUS       DACERL010000187         1455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.200, whole genome shotgun sequence.
ACCESSION   DACERL010000187 DACERL010000000
VERSION     DACERL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.200"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            191..454
                     /locus_tag="IXF54_002905"
     CDS             191..454
                     /locus_tag="IXF54_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HAQ1406097.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            454..789
                     /locus_tag="IXF54_002906"
     CDS             454..789
                     /locus_tag="IXF54_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1406098.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            863..>1455
                     /locus_tag="IXF54_002907"
     CDS             863..>1455
                     /locus_tag="IXF54_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HAQ1406099.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRG"
BASE COUNT          494 a          204 c          315 g          442 t
ORIGIN      
        1 aactcagaac tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
       61 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattgaa attgtttgat
      121 ggtggaaagt aacttcttgt attttggtgt atacaagtat atactaatgt gtgagagaag
      181 gagggattcc atgttagtaa aagaaatcga aatgaaaaaa tggggaaatt cacaaggtat
      241 tcgttttgga aaagaagaat tgtcagttct aggagaaact aatactgaaa atttaaaatt
      301 cgaaatggtg gtaacagaag gaaaaattgt tttaactcct aaaaataaaa agccagaaac
      361 tttagatgaa ttgttcgcca attatgaagg agaaccctta ggaaaagaag acaaatatga
      421 atggggaaat atgcaaggta gggagatcat ttaatgaaaa agaactatca tgatttatcg
      481 caaggagatc ttgtttatat tgatttaaat cctaccaaag gacatgaaca acggggaaaa
      541 cgaccatgta ttattttatc taaacctaat aaatatttga actatatgat tggtgtcgca
      601 ccgataacaa caaaagcgaa gccttttcct gtacatgttg atttgaatgg tacgcaaaaa
      661 ataaaaggac aaattctttt ggaacaccat cgaatgattg acattgagag tagaggtttt
      721 cagtttgtgg aagctgtgcc aaaggaaatt ttagaagaat gtattgaaaa aattaaatta
      781 ttttattaaa agcagcgaag aataattatt atttcttcgc ttttttgttg tttaaaatta
      841 aaaagttagg agtactgact atatggaaaa tgatacaaat atagttatgg aacatttgaa
      901 gcgtcgtgca aagcgacgct tatttttgtg gttgtttggt acttctgcag gtttgattac
      961 tattttgatc actgtgttcg taaccctttt tctaatactt gcggcgggtt cgattgataa
     1021 ttcggattcc gattcaagta gtggtggcga agcttttaca ggagagtatt cggaaggctt
     1081 accgatttat aaagaaatca aaggaagagg ccctttttct gatgagattg cgcaatatgc
     1141 cgtaggtgca gcagtgaaat ataaattatt accaagtgtg attctttcgc aatacggtta
     1201 tgaatcagcg tttggtacgt cggcgtccgc aagaaatgat ctcaactatt ttgggattac
     1261 ttggtttgat ggttgtctgt ttcctaaagg aactgcacga ggaattggtg gcattgaagg
     1321 gggctggtac atgaagtttc ccaactcaaa agctgcattc tcctattacg gctttatggt
     1381 agccacacaa tcaaatttta atgcatgtgt aggaaataaa agccctggcg caagcttatt
     1441 gatcttagga cgagg
//
LOCUS       DACERL010000188         1437 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.201, whole genome shotgun sequence.
ACCESSION   DACERL010000188 DACERL010000000
VERSION     DACERL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.201"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            135..>1437
                     /locus_tag="IXF54_002908"
     CDS             135..>1437
                     /locus_tag="IXF54_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1406100.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRR"
BASE COUNT          454 a          294 c          269 g          420 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgag
//
LOCUS       DACERL010000189         1436 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.202, whole genome shotgun sequence.
ACCESSION   DACERL010000189 DACERL010000000
VERSION     DACERL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.202"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            101..1402
                     /locus_tag="IXF54_002909"
     CDS             101..1402
                     /locus_tag="IXF54_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1406101.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          472 a          288 c          263 g          413 t
ORIGIN      
        1 gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca aaacatacag aagctccgtt
       61 agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcatat
      121 caaactacta cttggaatca aagataaaaa cattttcatc acaaatgtgg aatcgaagag
      181 cattaagaat atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg
      241 tcctttgtgt aaacagatga atcatgaagg catgatcgtt aagaacggaa agaaaaaatc
      301 gctcattcaa cttaataaat gtgcgaacca actgacttat cttgctttag cgaaacaacg
      361 ctaccactgt cgtggctgcc acacctattt tacggcgaat acctacattg tcgatagaaa
      421 ctgctttatc gctaaacaag tgcgctataa aattttagaa gagctaactg aaaaacaagc
      481 aatgacgaca attgctaagc attgcggcgt ttcttggtcg accgtttctc ggacactcgc
      541 ttctctgatc cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt tattaatgga
      601 tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc ttttcttgta tggatggcga
      661 tactggaaaa ttactggata ttcttccttc acggaagaaa aaggctctcg tttcttattt
      721 tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt
      781 ttcttatgcc tctctgataa aggaatgttt tccaaaggca aaactcgttg tggatcgttt
      841 tcacattgtc aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag
      901 ctttgatcga aacgatccaa cacaagccaa atattatcga caattaaagg cgttgtctcg
      961 gcttctgatc aaacgacagg acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt
     1021 tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga tctctgacga
     1081 attacgaatc gcctattttt actatcaaga aattcttcaa gcgtttcacg acaaagaggc
     1141 ggacactttc tttaaacttg taagaacgat gcccaattcc gttccaaaag aactgcattc
     1201 tataaaaaaa gcttttataa actatgaatc cggcattcgt ttagccctcg aattgcctta
     1261 ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac gagtcgctta
     1321 tggctttcga agctttcgga aaatgaagac cagaattttc ttactaaata atctgattac
     1381 ctatgaatca aaaaacatct aaagtagaaa aggaattcta ccttagatgt tcttat
//
LOCUS       DACERL010000190         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.203, whole genome shotgun sequence.
ACCESSION   DACERL010000190 DACERL010000000
VERSION     DACERL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.203"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(150..1337)
                     /locus_tag="IXF54_002910"
     CDS             complement(150..1337)
                     /locus_tag="IXF54_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1406102.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACERL010000191         1405 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.204, whole genome shotgun sequence.
ACCESSION   DACERL010000191 DACERL010000000
VERSION     DACERL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.204"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>1405
                     /locus_tag="IXF54_002911"
     CDS             <1..>1405
                     /locus_tag="IXF54_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406103.1"
                     /translation="LDYQISEGFYPTLIDLIRLDIFKDTDETMSVEESYRNEKILQVL
                     EEELESDQRKVNQVSEVEANEEVPWERPIFPKDESKEKEKGIIDEQADILTHKSIFEE
                     ENEVMGQLEVDPFEETMEDEETAEVEVDPVEANEDPTWTEGDSQEYIEPLISEEVEKN
                     ETEIQKATVFDYILDQYPPEHEWMKEKNKLFLNELYSKYQLPQTYQAFLESKEELISQ
                     GKGTLLEQLESVQSTNWHELAQEDLLATFEERQQETDQEINEYATEQSSNWEKAKEEL
                     NKEEQRVIEEEVQKIKERYEKKRDNAYSLCVEKINNFTQTNQEALSKEKENLLKERIK
                     ERKEEFYRGLRTDKLRISKELNEQLNKLYQTTTEVLMEKHEQIQQELQAQIPKWKKEY
                     EIHQQELKEQEEKQRKKEKEREQLELKKREQDLAEQKMAIEKQRLEQEKEKEAAARKE
                     RQEQMDTLRQAQLNYLT"
BASE COUNT          618 a          198 c          295 g          294 t
ORIGIN      
        1 cactggatta tcaaatttca gaaggttttt atcctacatt gattgattta atccgcctag
       61 atatatttaa agatacggat gaaaccatga gtgtggaaga gtcttatcga aacgaaaaaa
      121 ttttacaagt tttagaagaa gagttagaaa gcgaccaaag aaaagtgaat caagtgagtg
      181 aagtcgaagc aaacgaagaa gtaccatggg aacgcccaat ctttccaaag gatgaatcaa
      241 aagaaaaaga gaaaggaatt attgacgaac aagctgacat tttgactcat aaatcaatat
      301 ttgaggaaga gaacgaggta atgggtcaat tggaggtaga tccctttgaa gaaacaatgg
      361 aggatgaaga gacggccgaa gtagaagttg atccagttga agctaatgaa gacccaacat
      421 ggactgaagg ggattctcag gaatatattg aaccacttat cagtgaagaa gtagagaaaa
      481 atgaaacaga gatacaaaag gcaacggtat ttgattatat tttggatcaa tatccgccag
      541 agcatgaatg gatgaaagaa aaaaataaat tatttttaaa tgaactttat tcgaaatatc
      601 aattacctca aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta
      661 aaggcacact tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac
      721 aagaagattt attagctact tttgaagaac gtcaacaaga aacagatcaa gaaattaatg
      781 agtatgcgac ggaacagtcg tcgaattggg agaaagcaaa agaggaacta aataaagaag
      841 aacagcgagt gattgaagaa gaagtacaaa aaattaaaga gcggtacgaa aagaaacgtg
      901 acaatgcgta ttccttgtgt gtagaaaaga tcaacaattt tactcagacg aatcaagaag
      961 cattatcgaa agaaaaagaa aacttattaa aagaacgtat aaaagaacga aaagaagaat
     1021 tttaccgagg gttgcgtact gataagctaa ggattagcaa agaattaaat gaacaactga
     1081 ataaactcta tcaaactacg actgaagttc tgatggaaaa acacgagcag atccaacaag
     1141 aattacaagc acaaattcca aaatggaaaa aagagtatga aattcaccaa caagaattga
     1201 aagaacaaga agaaaaacaa cggaaaaaag aaaaagagcg cgaacaatta gaactaaaaa
     1261 aacgtgaaca ggatcttgcg gaacaaaaaa tggcgataga aaaacaacgg ctagagcaag
     1321 aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac
     1381 aagcacagtt aaattatctg acaca
//
LOCUS       DACERL010000192         1366 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.205, whole genome shotgun sequence.
ACCESSION   DACERL010000192 DACERL010000000
VERSION     DACERL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.205"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            82..>1366
                     /locus_tag="IXF54_002912"
     CDS             82..>1366
                     /locus_tag="IXF54_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1406104.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DACERL010000193         1342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.206, whole genome shotgun sequence.
ACCESSION   DACERL010000193 DACERL010000000
VERSION     DACERL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.206"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..824
                     /locus_tag="IXF54_002913"
     CDS             <1..824
                     /locus_tag="IXF54_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1406105.1"
                     /translation="DVDYAKSEMKQIRMLYGKDDRVQAHLLIQSFRPGEITAEKANQL
                     GAEYAKKIAPDHQTAIYTHTDKDHIHNHIVINSVNLETGKKFQAHGQAFLNKCYDLND
                     EICLAHNLPITERGKKPEKRTMSEIKLKEKNEPVWKDEIRIAIDQTMKNKKTRTYDQF
                     CNSLKTLGIHCFNRGKNFTYELIHKKKKVRSNRLGKDYEKETILHELDRRERTYNQQR
                     TNELRRARASIRETAEEVRREQRTLTPEQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1064..>1342
                     /locus_tag="IXF54_002914"
     CDS             1064..>1342
                     /locus_tag="IXF54_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406106.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQAL"
BASE COUNT          512 a          254 c          246 g          330 t
ORIGIN      
        1 gtgacgtcga ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg
       61 accgtgtaca ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa
      121 aagctaatca actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaacagcta
      181 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      241 tggagactgg aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc
      301 taaatgacga gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag
      361 aaaaacgaac catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg
      421 aaattcggat tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat
      481 tttgtaattc cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt
      541 acgaattgat ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa
      601 aggagacgat tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca
      661 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      721 aacgaactct tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac
      781 acgttgaaag aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata
      841 gaaaacgaaa taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa
      901 actacgattg atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag
      961 gaacagaaaa aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta
     1021 gtcctgacac tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc
     1081 ctatctactc atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca
     1141 aacaagatat tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg
     1201 tatattttcc ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg
     1261 cgtttctaga agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa
     1321 aaagaaaaaa gagcaagcat ta
//
LOCUS       DACERL010000194         1340 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.207, whole genome shotgun sequence.
ACCESSION   DACERL010000194 DACERL010000000
VERSION     DACERL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.207"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            217..492
                     /locus_tag="IXF54_002915"
     CDS             217..492
                     /locus_tag="IXF54_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406107.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            534..956
                     /locus_tag="IXF54_002916"
     CDS             534..956
                     /locus_tag="IXF54_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406108.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            976..>1340
                     /locus_tag="IXF54_002917"
     CDS             976..>1340
                     /locus_tag="IXF54_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406109.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQ"
BASE COUNT          533 a          176 c          256 g          375 t
ORIGIN      
        1 atcactttct tacgaaaggt gctaggtagg ctgtgcctac cacgtttcta agaaagataa
       61 atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg
      121 cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta
      181 cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat tagaaatatt
      241 gaagaaaagt acattaataa aattgacaaa cgttgtgaag aacttacggt caagactggc
      301 aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt tgatcacgct
      361 cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt ggatattcaa
      421 acttataata caaaaacttt agaagaatat attgcaacaa ataatcaatt aattggtttg
      481 ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca attttggcaa
      541 aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt gatgaaataa
      601 tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg tgtatgatgt
      661 atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat gcaatgggaa
      721 aagaaatttc tattttaacc gagatggttt cagagtttgc ggatcttcat ttgaaagata
      781 cagggatttt aattggtaca aattcaactg tttaccaaga agcaaaagag cgtgtggaag
      841 ggaaaataaa agcgaatcaa acgaagaagt attctcctaa aatcaagaaa tcaataaatg
      901 aatctgttca tgccagagta gacccgtttg atccaacaaa gtttttaaaa agataaaaag
      961 tagaagggga acatgatgaa taaatcgcct gcagtcattc tcacttgtca atttaccttg
     1021 cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa agcgctggaa
     1081 gaaatggaac gacgtacgcc tgaagaagaa caggaactgc aacgaatcaa gaatgcttta
     1141 gaggattttt atattcctga aggagaaact ttttcggaga gtaagacgga tcaagaattc
     1201 agcgaaacgt cgaatgaagc tcaatctttg atgggaaatg gaatcaactt tgacaagtta
     1261 gaggaacaag attttaccaa atatctttct tatatgaccc gtcactatgc gttagaacaa
     1321 aagaaagaac tgacgcaaag
//
LOCUS       DACERL010000195         1326 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.208, whole genome shotgun sequence.
ACCESSION   DACERL010000195 DACERL010000000
VERSION     DACERL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.208"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..347)
                     /locus_tag="IXF54_002918"
     CDS             complement(<1..347)
                     /locus_tag="IXF54_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1406110.1"
                     /translation="MKLLNQKTVIAGTFAVTLAATLALAGSVDAASTNVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGP"
     gene            complement(487..693)
                     /locus_tag="IXF54_002919"
     CDS             complement(487..693)
                     /locus_tag="IXF54_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406111.1"
                     /translation="MYADKNEIEWLLFESGISVKDIYKESGVARTTVIDLINKTSSID
                     KMRFDNAVKLTKMAKMRKNLYENK"
     gene            881..>1326
                     /locus_tag="IXF54_002920"
     CDS             881..>1326
                     /locus_tag="IXF54_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406112.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPDPVQISVKRQKDYKGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKY"
BASE COUNT          447 a          199 c          242 g          438 t
ORIGIN      
        1 ggcccttcat aagtccagcc gtaattttta gtcaaccatt tcacttcatc ccaactagct
       61 gtgtaatggt gatcacgaag acctttattg tataaacgat agacaagatg accaccgcta
      121 gtcggtgctt cccaagcggg gccttcataa ttaccccact taattttcac taggtgatca
      181 cgttcttttg tgctagaggt gtaaaaatgt tcgtggttat ttggattgta aaggcgatac
      241 atgacgttag ttgaagcagc gtcaacagat ccagcaagag caagtgtggc agccagtgtg
      301 acagcaaatg ttccagctat aacagttttt tggtttaata atttcatgtt ttcaactcct
      361 aaatagatta aatgttttta caagttaatt atatgtcggt ataccggcaa agtcaagagt
      421 aaatgacggt ataccgatta aaatgcaata agaaaccaat acttttgaat aagcattggt
      481 ttcttattat ttgttttcat ataagttttt tcgcattttc gccattttag tgagtttgac
      541 tgcattatca aaacgcattt tatcgattga ggaggtttta ttaatcaagt cgataactgt
      601 agtgcgcgcg acacctgatt ccttatatat atctttaacg gagattccac tttcaaataa
      661 taaccattcg atttcatttt tatctgcgta cattgcaatg ctccctcttg attttttctt
      721 tattatagct cttttttgta ataaatcaca atatcaagag aaaaagtttc tatagaaagg
      781 tgggaattta tgagtatccg ttccccgtgt attaaaatga ggtttgttgc tactttcgtt
      841 atttgatata ctaaaataaa acaacgaaag aaggaataaa atgaagaaaa aacaactatg
      901 gtttgtctct atgtttgcaa tattttttat tattttaagt ggatgttcaa gtaataagaa
      961 atcggaagct aaagaagata atagtttatt gcaaccagga actgtttgga aagaagaagt
     1021 aggcggtctt gttaataatt tgaaaattat agacgatact acatgggaat attctgaaag
     1081 tgtatggcat ccagatcctg tacaaatatc agttaagaga caaaaagact ataaaggttt
     1141 agaaagatac aaaatagtag attctgcagg tattagggaa tttataaaca aacctgattc
     1201 attatttatt gttgttccct atgagaagga tggcgtaaaa aaaataattt ttcttggatc
     1261 tagtaaggat gaaaaacgaa cgaaagagaa attaattcat gatggatcac aatcaaataa
     1321 atacaa
//
LOCUS       DACERL010000196         1313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.209, whole genome shotgun sequence.
ACCESSION   DACERL010000196 DACERL010000000
VERSION     DACERL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.209"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            200..742
                     /locus_tag="IXF54_002921"
     CDS             200..742
                     /locus_tag="IXF54_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406113.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFKKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="IXF54_002922"
     CDS             898..1272
                     /locus_tag="IXF54_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1406114.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          255 g          378 t
ORIGIN      
        1 attgatgaag cagttcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttaaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DACERL010000197         1300 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.210, whole genome shotgun sequence.
ACCESSION   DACERL010000197 DACERL010000000
VERSION     DACERL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.210"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            184..492
                     /locus_tag="IXF54_002923"
     CDS             184..492
                     /locus_tag="IXF54_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406115.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            494..916
                     /locus_tag="IXF54_002924"
     CDS             494..916
                     /locus_tag="IXF54_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406116.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            936..>1300
                     /locus_tag="IXF54_002925"
     CDS             936..>1300
                     /locus_tag="IXF54_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406117.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQ"
BASE COUNT          516 a          173 c          248 g          363 t
ORIGIN      
        1 cccactttta aaaaaagtgg ttggataggc tgtgcctatc acgtttctga aaaaaataaa
       61 tgaccccaaa ataccaatat ataggatagt acaaggatag caaaggatag taaaaggata
      121 gaggtaggat agtgaaaagg ataggctcct tttcactatc ctatattata ttggaggata
      181 tttatgaatg ttaaaattag agatttagat ccaaaattca tttctgaaat tgatcgaaga
      241 tgcgttgaac taagtaatcg tacaggaaat aaatggagtc gtaatgatta tttaaagcta
      301 ctcgttgaaa atgattttga tcgtcctttg atggaatata aacaagataa atttgatcaa
      361 ttattaaatc aattaataga taaattttct tttgttcaaa gtcataatac aaaagtatta
      421 gaagaatata tttgtcagaa taatcaaatt atcgaacttt taattgaaca aagtagagga
      481 ggagaagttt aacatggcca aagttataga caagcatatt tatttttatg aaacacatat
      541 agatgttcta gatgaaatta ttgcaactca tgcagaggtt aaaaactata gtgaagccgt
      601 tcggtttcta tgtatgaatt atgatccaga agtggagaaa aaagtaaatt ccaatcaagc
      661 aaagttgaac ggaatgagta aagaaatttc tattttaacc gaaatggttt cagagtttgc
      721 ggatctccat ttgaaagata cagggatttt aattggtaca aattcaactg tttaccaaga
      781 agcaaaagag cgtgtggaag gcaagataaa agcaaatcaa acgaagaaat attctcctaa
      841 gataaagaaa acaataagcg aatcagtgac tacaaaaagt gaagtatttg atccaacaag
      901 gttcttaaaa cgataaaaat tagaaggaga atgtgatgag taaatcacct gcagtcattc
      961 tcacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga
     1021 caagagaaaa agcgctggaa gaaaaggaac gacgtacgcc cgaggaagaa caggaactgc
     1081 gtctaatcaa gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaga
     1141 gtaagacgga taaagaattc agcgaaacgt cgaatgaagc tcaatctttg atgggaaatg
     1201 gaatcaactt tgacaagtta gaggaacaag attttaccaa atatctttct tatatgaccc
     1261 gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
//
LOCUS       DACERL010000198         1297 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.211, whole genome shotgun sequence.
ACCESSION   DACERL010000198 DACERL010000000
VERSION     DACERL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.211"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            141..398
                     /locus_tag="IXF54_002926"
     CDS             141..398
                     /locus_tag="IXF54_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406118.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXF54_002927"
     CDS             complement(630..1043)
                     /locus_tag="IXF54_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1406119.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          492 a          169 c          161 g          475 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca cacataaaaa taaaaaacat
     1141 ttgtatttta tcaaagtttt attatacttg tcaatttgtg tacatcctaa aatataagct
     1201 atttgtttac ttctgtagaa tatatacttt gttgttagat aagttgcaca tgtttataga
     1261 atctaaataa taaattttta tgtaccgccc ctccaaa
//
LOCUS       DACERL010000199         1277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.212, whole genome shotgun sequence.
ACCESSION   DACERL010000199 DACERL010000000
VERSION     DACERL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.212"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>1277
                     /locus_tag="IXF54_002928"
     CDS             <1..>1277
                     /locus_tag="IXF54_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406120.1"
                     /translation="PKTVTKVLQDYGNYLQYQNVFSWIYNTFLWGLIKGLYNLNTLLE
                     KTIYQSFDLKDLLNAAGVNELFQSLISKVLALFCVVTLIYLGLKFFMSKHPPKIKNIL
                     VNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAANKADKTGSPAFQIIQNNVIDVSTL
                     LENDPNKVEHIPTDKRNALTAKNFKTANINGIITPDQAKKMSESSDISTVQKDRLKAL
                     QYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRYISSPGIILAGESSLSVAYLFILFT
                     TVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILELRIYTLMLS
                     GIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNSLLSMFALG
                     NIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFG"
BASE COUNT          421 a          183 c          247 g          426 t
ORIGIN      
        1 atcccaaaac cgtaacgaaa gtcttacaag attatggtaa ttatctgcag tatcaaaatg
       61 tattcagttg gatttataat acttttttat ggggtttgat taaagggtta tataatctca
      121 atactttact agaaaaaaca atttatcaaa gctttgattt aaaagactta ttgaacgccg
      181 ctggtgttaa tgaactgttt caaagtttga ttagtaaggt attggctctt ttttgtgtcg
      241 tcacattaat ttatttaggc ttaaaatttt ttatgagtaa acatccacca aaaatcaaaa
      301 atatcttggt taatctattt atggccatga ttttgatttt tggtggtagt tctttaatcg
      361 atgaagggct aaatattagt aaaaactttt acggagacgt gactgcagca aataaagcag
      421 ataaaactgg ttcacctgct tttcaaatta ttcaaaataa tgtgattgat gtgagtacgt
      481 tactagaaaa tgatccgaac aaagtagaac acataccaac agacaaaaga aatgctctca
      541 cggcgaaaaa ttttaaaaca gcgaatatca atgggattat tacacctgat caagctaaga
      601 aaatgtctga atcaagtgat attagtacag tacaaaaaga tcggttaaag gcattacagt
      661 atcgactaga gttagacgat aatgggaaag aaatccctgt tcaaattagt gatgaaggtt
      721 tagcaaaata tgtgtatact tcaggctatc gccgatacat tagtagtcca ggtattattt
      781 tagctggtga atcttctttg tcggtagcct atttatttat cttgtttacg actgttactt
      841 gcattattga gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg
      901 agacaggaca acggatgaaa agtgctatag aagatatttc tcaatccttc ttgttattgg
      961 catttacgat tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc
     1021 atgcggcagg aaagatcaat ggattcttgt atgttgtcgc tttaattgta ttaaccattg
     1081 cgctattcaa aggctctcaa tcagttacga aaattttcgg cgttgatacg agtctgaaaa
     1141 acggcagcaa ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta
     1201 gcggggctaa aaaccttgca agtgctggta agagtggttt aagcaattta aatgatattc
     1261 gtaaaaatgg atttggt
//
LOCUS       DACERL010000200         1273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.213, whole genome shotgun sequence.
ACCESSION   DACERL010000200 DACERL010000000
VERSION     DACERL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.213"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..1149
                     /locus_tag="IXF54_002929"
     CDS             <1..1149
                     /locus_tag="IXF54_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1406121.1"
                     /translation="RLFLWLFGTSAGLITILITVFVTLFLILAAGSIDNSDSDSSSGG
                     EAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTS
                     ASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSN
                     FNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSIITSNKLTEYDEFAIKHWG
                     EGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHP
                     GSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVK
                     VGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDPLPFLNSSKK"
BASE COUNT          373 a          214 c          309 g          377 t
ORIGIN      
        1 cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt gattactgtg
       61 ttcgtgaccc tttttctaat acttgcggcg ggttcgattg ataattctga ttcagattca
      121 agtagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat ttataaagaa
      181 atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgctgtagg tgcagcagtg
      241 aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttatgaatc agcgtttggt
      301 acgtcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt tgatggttgt
      361 ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg gtacatgaag
      421 tttcccaact caaaagctgc cttctcatat tacggcttta tggtagccac acaatccaat
      481 tttaatgcat gtgtaggaaa taaaagccct ggcgcaagtt tattgatctt aggacgaggc
      541 ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggatg tatgtccatt
      601 atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg gggtgaaggt
      661 ggcaataaca acggcacgat cactggtgaa tggacgaatc cattccctgg cagcagttta
      721 gataaaaatt ccttcagtgg tggtcaatta tttggaacaa atccaggcgg tgaattccgt
      781 ccgaatggct tccatgatgg attagacttt ggttcagtcg atcatccagg aagtgaaatt
      841 cacgcggtgc atggaggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttaggt
      901 gcatgtgtga tcgtgattaa ttacgacggt ttgaatatgg tatatcaaga atttgcgaat
      961 agtacaggaa attctcgtgt gaaggttggc gatcaagtga aagttggtca agtaatcggg
     1021 atacgagata cagcacactt gcatttaggt ttcactcgaa tggattggcg tcaagcacaa
     1081 ggacacgcat tcacagacga tggtacatgg attgatccat taccattttt gaatagcagc
     1141 aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt cttaacagct
     1201 gcaattagcg ttagtttaat cggcaatatc gcgttgtttt ggcagctgaa agaagaacaa
     1261 gagacaaata cct
//
LOCUS       DACERL010000201         1249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.215, whole genome shotgun sequence.
ACCESSION   DACERL010000201 DACERL010000000
VERSION     DACERL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.215"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..978
                     /locus_tag="IXF54_002930"
     CDS             <1..978
                     /locus_tag="IXF54_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1406122.1"
                     /translation="FDALTVNTMNKYKSFKITDTFDNKNFDMVSDGKKYDGALWYVEA
                     NGTWKKADTGGKLTVSGNTVTYTASDSFLANAANYGRDYILQLHMRAKNDAKGIAPNV
                     ATVTFNNDPNKGTQKTNTVENYFSLKPNKKVEQDGKDVNGRNKGDNGPATEPLNAGSE
                     VQYLVTQKWHTKGVDIALDHYKQFSIQDPIEDRMTYQEGSAQVIDQSTGKDITSEGVL
                     TYDSHSRTLKWEASSDFLNNNFLDGREVQLIFTARTPLNKQVDIDNQANVSVENIEKE
                     TNVVTIGVEPNLPQVIVPKTGSNHLVLTVIGSILFLTLASLGYVVLKLK"
     gene            complement(1016..>1249)
                     /locus_tag="IXF54_002931"
     CDS             complement(1016..>1249)
                     /locus_tag="IXF54_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1406123.1"
                     /translation="YGAKGENISQTGGYIDNLLSDDGAIDAMLGWKNSPAHNANLLYN
                     NQSILGLGHNFKIYSDGSLGIDNVFISANPIFK"
BASE COUNT          459 a          206 c          231 g          353 t
ORIGIN      
        1 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
       61 tttgacaaca aaaattttga tatggtatcg gatgggaaaa aatatgatgg cgcattgtgg
      121 tatgttgaag cgaatggtac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
      181 ggtaatacag tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt
      241 agggattata ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
      301 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca
      361 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgta
      421 aacggacgta ataaggggga caatgggcca gcaactgaac cattaaatgc aggatctgaa
      481 gtacaatatc tagtcacaca aaaatggcat acaaaaggcg tagacattgc gctcgatcat
      541 tacaagcaat ttagtattca agatccaatt gaagatcgta tgacgtatca agaaggttct
      601 gcgcaagtga tagatcaaag tacaggaaaa gatattactt ctgaaggcgt attaacctat
      661 gatagccatt caagaacgct gaagtgggaa gcttcctctg atttcttaaa taacaatttt
      721 ttagacgggc gagaggttca actgatattt acagctagaa ctcctttgaa taaacaagtt
      781 gatatcgata accaagccaa tgtttcggta gaaaatattg agaaagaaac taatgtagta
      841 acgattggcg tagaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
      901 ttagtactaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
      961 gttttaaaac ttaaataaat acaaaaacaa ggctgaaaat cagccttgtt ttcttttatt
     1021 tgaaaattgg attcgcgcta ataaatacat tatctatgcc taatgaacca tcagaataaa
     1081 ttttaaaatt atgacctaat ccgagaatac tttggttatt gtaaagcaag tttgcattat
     1141 gggcaggact atttttccat cctaacatgg catcgatagc accatcgtct gataaaaggt
     1201 tatcaatata accaccagtt tggcttatgt tttctccctt agcaccata
//
LOCUS       DACERL010000202         1249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.216, whole genome shotgun sequence.
ACCESSION   DACERL010000202 DACERL010000000
VERSION     DACERL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.216"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..978
                     /locus_tag="IXF54_002932"
     CDS             <1..978
                     /locus_tag="IXF54_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1406124.1"
                     /translation="FDALTVNTMNKYKSFKITDTFDSKNFDMVLDGKNYDGALWYVEA
                     NGTWKKADTGGKLTVSSNTVTYTASDSFLANAANYGRDYILQLHMKAKNDAKGIAPNV
                     ATVTFNNDPNKGTQRTNTVENYFSLKPNKKVEQDGKDVDGRNDGKKGTPTAPLNAGSE
                     VQYLVTQKWHTKGVDIALDHYKQFSIQDPVEARLTYKEGSARVIDMSTGKDITSEGTL
                     TYDSNSRTLKWEASADFLSKNLLDGREIQLIFTAKTPLNEELDIDNQAKVSVESIEKA
                     TNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGSILFLTLASLGYVVLKLK"
     gene            complement(1016..>1249)
                     /locus_tag="IXF54_002933"
     CDS             complement(1016..>1249)
                     /locus_tag="IXF54_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1406125.1"
                     /translation="YGAKGENISQTGLYIDDLLSDDGAIDAMVGWKNSPAHNANLLYN
                     NQSILGLGHNFVVDSAGRIGVDNVFISANPIFK"
BASE COUNT          455 a          209 c          234 g          351 t
ORIGIN      
        1 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
       61 tttgacagca aaaattttga tatggtattg gatgggaaaa actatgatgg cgcattgtgg
      121 tatgttgaag cgaatggtac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
      181 agcaatacag tcacttatac tgcttctgac agctttctag ctaacgctgc aaattacggt
      241 agagattata ttttgcagct ccatatgaaa gcaaaaaatg atgcaaaagg aatcgcaccc
      301 aatgtggcta ctgtaacgtt taacaatgat ccgaataaag ggacacaaag aacaaataca
      361 gtagaaaatt atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
      421 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
      481 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
      541 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
      601 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat
      661 gatagcaatt caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt
      721 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
      781 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
      841 acgattgggg taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat
      901 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
      961 gttttaaaac tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt
     1021 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
     1081 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
     1141 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
     1201 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccgta
//
LOCUS       DACERL010000203         1239 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.217, whole genome shotgun sequence.
ACCESSION   DACERL010000203 DACERL010000000
VERSION     DACERL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.217"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>1239
                     /locus_tag="IXF54_002934"
     CDS             <1..>1239
                     /locus_tag="IXF54_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1406126.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSR"
BASE COUNT          406 a          221 c          268 g          344 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgta
//
LOCUS       DACERL010000204         1224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.218, whole genome shotgun sequence.
ACCESSION   DACERL010000204 DACERL010000000
VERSION     DACERL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.218"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..232
                     /locus_tag="IXF54_002935"
     CDS             <1..232
                     /locus_tag="IXF54_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1406127.1"
                     /translation="CKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFY
                     HNCGFKIGSVDTMLYANFENNFEKAVFWYLRF"
     gene            325..1119
                     /locus_tag="IXF54_002936"
                     /note="aph(3')-IIIa"
     CDS             325..1119
                     /locus_tag="IXF54_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1406128.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          385 a          210 c          296 g          333 t
ORIGIN      
        1 atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg
       61 ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc
      121 ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc
      181 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
      241 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
      301 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
      361 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
      421 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
      481 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
      541 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
      601 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
      661 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
      721 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
      781 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
      841 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
      901 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
      961 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     1021 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     1081 aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa
     1141 aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt
     1201 ccatactctg atgttttata tctt
//
LOCUS       DACERL010000205         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.219, whole genome shotgun sequence.
ACCESSION   DACERL010000205 DACERL010000000
VERSION     DACERL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.219"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            414..947
                     /locus_tag="IXF54_002937"
     CDS             414..947
                     /locus_tag="IXF54_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1406129.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACERL010000206         1179 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.220, whole genome shotgun sequence.
ACCESSION   DACERL010000206 DACERL010000000
VERSION     DACERL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.220"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            66..800
                     /locus_tag="IXF54_002938"
     CDS             66..800
                     /locus_tag="IXF54_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ1406130.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          445 a          183 c          175 g          376 t
ORIGIN      
        1 aaataagtgg ttataatgga ttgttaatat aaacattcat tataacctca taggagtggg
       61 ttataatgaa caaaaaaaat ataaaagata gtcaaaactt tattacttca aagcatcata
      121 taaatgaaat tttgagaaat gtacatttaa atacaaatga taatattatt gagattggtt
      181 cagggaaagg tcatttcacg tttgaattag ctaaaaggtg taattatgta accgccattg
      241 aaatagatcc taaattatgt aggataacta aaaacaaact tattgaatat gagaacttcc
      301 aggttatcaa taaagatatt ttacaattta agtttcctaa aaataagtca tataagatat
      361 atggaaatat accctacaat ataagtacag atataattcg aaaaattgtt tttgaaagca
      421 cagctacaga aagttattta atagtggaat atggatttgc taaaaggttg ctaaatacaa
      481 atcgttcact agcactattt ttaatgacag aagttgatat atccatatta agtaaaatcc
      541 ctagagaata ctttcatcca aaacctagag ttaatagctc gttaattgta ttaaaaagac
      601 acccttcaaa aatatcactc aaagatagaa aacaatatga aaattttgtt atgaaatggg
      661 ttaacaaaga atacataaaa ctattttcca aaaaccaatt ttatcaagcc ttaaaatatg
      721 caagaattga cgatttaaac aatattagct ttgaacaatt cttgtctctt ttcaatagct
      781 ataaattatt taatagataa gttaaggggt gcataaactg catcccttaa cttatcttat
      841 tttgcttaaa caatcttggc ccgtaaactg cataactcat atgtaaatcg ctccttttta
      901 ggtggcacaa atgtgaggca ttttcactat ttccggcaat tacttccatg taaagtataa
      961 cccactatac tttacttcgt aaaggtgggc tcgccgaggg gtcttgcaga cggctctgtc
     1021 ggctcttttg agccgagtat cagcgcaccc ttatggtgca attagctgat acaaaaaaca
     1081 aagagttggc tcatgtaatc caactcttaa aacacaaaaa aataaccccc ttggcttttc
     1141 agcagggggc atatttttta tttaattcct cactcttta
//
LOCUS       DACERL010000207         1131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.221, whole genome shotgun sequence.
ACCESSION   DACERL010000207 DACERL010000000
VERSION     DACERL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.221"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            102..>1131
                     /locus_tag="IXF54_002939"
     CDS             102..>1131
                     /locus_tag="IXF54_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1406131.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAV"
BASE COUNT          345 a          219 c          223 g          344 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta a
//
LOCUS       DACERL010000208         1095 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.222, whole genome shotgun sequence.
ACCESSION   DACERL010000208 DACERL010000000
VERSION     DACERL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.222"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            44..319
                     /locus_tag="IXF54_002940"
     CDS             44..319
                     /locus_tag="IXF54_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406132.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(777..>1095)
                     /locus_tag="IXF54_002941"
     CDS             complement(777..>1095)
                     /locus_tag="IXF54_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1406133.1"
                     /translation="SLIDIKSPIITDMPKAPKHGNKAEDAIIQMMDIETERDAILAAL
                     MALSLISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGKII
                     AYK"
BASE COUNT          356 a          211 c          175 g          353 t
ORIGIN      
        1 cgagtggtcc gctgaaatat aaaacaagga tgtgcaagac aacatgtata aaccctacat
       61 gattagttat gatcttaata acccagggca aaagtatgat aaagtatttg aaataataaa
      121 agaatttggg gcatatataa agctacaaaa atctttttgg ttagttaaaa ctaatttgaa
      181 tccaaatcaa atgtgtgaaa aattaaatac agtacttgat aataacgatt ccttattcat
      241 ctgtgaactg caaaaaaatt atcaaggtag agctacagag gaaaattgga aattcattaa
      301 cgaacatatt ttctcttagt aaggattaga tattttttct ctgtttaaac aattgcaaat
      361 accgtcaata tctgaattag aaacacttac cttttgctcc ttgctactac caatagcttg
      421 gagcaattct tgtatttctt ctggagtgcc ttctactgat aatttcattt ttcttccctc
      481 caatacataa attaatagac agcagcagtt gaaagacgac aagaacatgt ttagttttgt
      541 gagtgctgct gtctaatcaa aataaacagc aaccgatgaa gaatttagga ggagttgaat
      601 tcacatcctt ttcttcataa ttagttgctg tctatctaag aagaagttta aaacgatgag
      661 ggagattgcc tcccttcgtt tattttgtcg atcctgtttc ctaatctttc gacactacca
      721 taataacact ggtaaatgtc taaaaaccgc catcattccg ccaaaaaacc gccaaattat
      781 ttataagcaa ttatttttcc gtgtttatat gcttctgcaa actctattag agcttccgac
      841 ttcatccgtt gtatacttct ttctgaataa cccacttcac ggctaattct gtagtttgag
      901 aagctatctg gcacacagaa gctgtagtag agtatctgac gactaatcag actaagagcc
      961 atcaaagccg ctagaatcgc atctctctcc gtttctatat ccatcatctg aataatcgcg
     1021 tcttctgcct tattgccatg cttcggtgcc ttcggcatat ccgttataat cggcgactta
     1081 atatctatca aagag
//
LOCUS       DACERL010000209         1093 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.223, whole genome shotgun sequence.
ACCESSION   DACERL010000209 DACERL010000000
VERSION     DACERL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.223"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(275..868)
                     /locus_tag="IXF54_002942"
     CDS             complement(275..868)
                     /locus_tag="IXF54_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406134.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(869..>1093)
                     /locus_tag="IXF54_002943"
     CDS             complement(869..>1093)
                     /locus_tag="IXF54_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406135.1"
                     /translation="AKMVNNLVHLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFA
                     IIESIFFADKNHYHALDPNRNYHGTYKEDE"
BASE COUNT          287 a          224 c          173 g          409 t
ORIGIN      
        1 catacccttt ggattttaaa atttctattt cccgatttcc tagttgcttt aatggatcaa
       61 gtactttttg atcattttgt ggtgaaaaag ctgttttaat tgcttctaac atgtacagtc
      121 acctcttctt gtttgattct ttacttggtt aaaagattgc ccttttccca acttacccag
      181 gcttaaagtg tgaggaaatt ttctagtggt ctcttccttc ggaacatctt tttctaacca
      241 tatcaacgga tatttcattc tctcttctcc attcctactc atcattcata ttgttcattc
      301 gatacagaat cttgatcaat tcttttttgt agagtggttt taaccctaaa atgccactag
      361 atcgcattaa ctgacggttt ctggtttgta aatccttgat tgtctttcct tcaatgacga
      421 aaaagaagct taagtcagga cgttcttgtt ctgctcgttt caattcctgg atttcttcga
      481 ctaatttctt ttcatatttt tttaatcgtt gtagatttct tcctaaggac tgttccatac
      541 gatttttatc taataattgc cgcttttgca cgatttgttc cgttagatcc gtctctgtgg
      601 tcaaactaac taacttaatt gttggcacat acgtacgcat gacggtggtc aactgattca
      661 ttcgttctag tttctcttgt tcatttaatc cttccacgtc tgtagaaact acacgataat
      721 aacgttgaaa atgatcttct ctagtaatta agactccctc ttctgtacag gatttatacg
      781 gaaataagtc caagatagag cgctcttctg gttttccaaa ctgcaaaatg gatttccttt
      841 caactgaatt ttgtcctttg actcgcattt attcatcctc cttatacgta ccatgataat
      901 ttcggttagg atctaacgca tgataatggt tcttgtccgc aaaaaaaata gactcgataa
      961 tcgcaaaaat ttgtcgttga cctgggttat tatagggtcg ccaaacaatc actaacgcta
     1021 tcgcaatcca taagccataa ttcaagagtg caaaaaagag tggtaagtgg acaagattat
     1081 tgaccatttt cgc
//
LOCUS       DACERL010000210         1089 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.224, whole genome shotgun sequence.
ACCESSION   DACERL010000210 DACERL010000000
VERSION     DACERL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.224"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..131
                     /locus_tag="IXF54_002944"
     CDS             <1..131
                     /locus_tag="IXF54_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1406136.1"
                     /translation="GTEAKKEQSVEEILNDVEEKRAEKIAAFTAALNKTFGQGDAK"
     gene            131..>1089
                     /locus_tag="IXF54_002945"
     CDS             131..>1089
                     /locus_tag="IXF54_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1406137.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVNNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAEPITALDAATMGGLMARLSKFNIEGVDDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDY"
BASE COUNT          387 a          168 c          239 g          295 t
ORIGIN      
        1 caggtaccga agctaagaaa gaacaaagcg tcgaagaaat tttgaatgac gttgaagaaa
       61 aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt ggacaaggag
      121 atgcaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg accaattgat
      181 gaaagctttt aaagatggta acgaagagtc ttttagcgca gctatggtaa gtttatctaa
      241 ggaaatacag gataaaattt tagaagaagc aacagcaaaa aatcaagatc aattagtatt
      301 aatgaaccgt gggcagcgtg tactaactac acaagaaaca aaattctata acgaagtagt
      361 gaataatgaa ggttttgcag gagttgaaga attagtacca gctactgtat ttgaacgcgt
      421 atttgaagat ctagaacaat ctcatccact attgcaaaaa attacttttg ttaacacaac
      481 tggtgtaaca gaatggatcg tgtcacgtgg agtcaatcca gcatggtggg gcaaactttg
      541 cgaagctgtt aaaaaagttt tagataatgg ctttgacgta attaacatga agcagttcaa
      601 gctatcaggt tatattcctg tatgtaaggc aatgcttgac ttaggtccag tatggttaga
      661 tcgttatgtt cgtactgttt tagttgaatc attgagaatt gcattagaac aagcaattgt
      721 tgatggtact ggtaaagata tgccagtcgg aatgatgcgc gacatgagca aacaaactag
      781 cggagaatat gctgaaaaaa cagcagaacc tattacagct ttagatgctg caactatggg
      841 cggtttgatg gcacgactat caaaattcaa tatcgaaggt gtagatgatc cgatttatcg
      901 taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact ggtctaaagt
      961 attcccagct aagactgtac taactgctaa cggtgaatac gtacaagtat taccagtacc
     1021 agtttcagac ttgcagtcaa ctgctgtgcc agaaggaaaa gcagttattg gggtagcttc
     1081 agattactt
//
LOCUS       DACERL010000211         1089 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.225, whole genome shotgun sequence.
ACCESSION   DACERL010000211 DACERL010000000
VERSION     DACERL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.225"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..131
                     /locus_tag="IXF54_002946"
     CDS             <1..131
                     /locus_tag="IXF54_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1406138.1"
                     /translation="GTEAKKEQSVEEILNDVKEKRAEKIAAFTAALNKTFGQGDVK"
     gene            131..>1089
                     /locus_tag="IXF54_002947"
     CDS             131..>1089
                     /locus_tag="IXF54_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1406139.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDY"
BASE COUNT          389 a          158 c          240 g          302 t
ORIGIN      
        1 caggtactga agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa
       61 aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt ggacaaggag
      121 atgtaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg accaattgat
      181 gaaagctttt aaagatggta acgaagaatc ttttagcgca gctatggtaa gcttatctaa
      241 ggaaattcag gataaaattt tagaagaagc aacagcaaaa aatcaagatc aattagtatt
      301 aatgaaccgt ggtcagcgtg tattaactac acaagaaaca aaattctata acgaagtagt
      361 gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca gctactgtat ttgaacgtgt
      421 atttgaagat ttagaacaat ctcatccact attgcaaaaa attacttttg ttaacacaac
      481 tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg
      541 cgaagctgtt aaaaaagttt tagataatgg ctttgatgta attaacatga agcagttcaa
      601 gctatcaggt tatattcctg tatgtaaggc aatgcttgat ttaggtccag tatggttaga
      661 tcgttatgtc cgtactgttt tagtagaatc attgagaatt gcattagaac aagcaattgt
      721 tgatggtact ggtaaagata tgccagtcgg aatgatgcgt gacatgagca aacaaactag
      781 cggagaatat gctgaaaaaa aagcagaacc tattacagct ttagatgctg taactatggg
      841 cggtttgatg gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg
      901 taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact ggtctaaagt
      961 attcccagct aagactgtac taactgctaa tggagaatac gtacaagtat tgccagtacc
     1021 agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg gggtagcctc
     1081 agattactt
//
LOCUS       DACERL010000212         1075 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.226, whole genome shotgun sequence.
ACCESSION   DACERL010000212 DACERL010000000
VERSION     DACERL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.226"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..548
                     /locus_tag="IXF54_002948"
     CDS             <1..548
                     /locus_tag="IXF54_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1406140.1"
                     /translation="LLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQK
                     FVSHLLEVVPGQIQSIDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAAC
                     TSEACDFKCWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKEDFTT
                     GFEFSEKKKTNFKKRTRRTTK"
     gene            789..>1075
                     /locus_tag="IXF54_002949"
     CDS             789..>1075
                     /locus_tag="IXF54_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HAQ1406141.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHG"
BASE COUNT          397 a          191 c          226 g          261 t
ORIGIN      
        1 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca
       61 aatgggaaag ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag
      121 ccaatatcca aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca
      181 ttgattttgg atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc
      241 gtcatttagg aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg
      301 cagcttgtac gtctgaagcc tgtgatttta aatgttggac aacgattgcg aaaaaaaaac
      361 tcacagcgac acagctgaag gagatcattc aaaatggccg aacaagccaa ccagtgaaag
      421 ggttaaaagg tcagaagggt tcgtttgaag caacgatcgt tttaaaagaa gacttcacta
      481 caggctttga attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacta
      541 caaaataatg gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa
      601 atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg tgccagcaaa cgaagactgc
      661 agcaattaaa gaaccagcag cggattcaaa aagaaattga attagatcga tagacattaa
      721 ccttgttaca aatacactat catgatatat ttaatgtagt gtatttgtaa cgaaaggagg
      781 ggaagtaaat gcaagataaa ctgaaagagt tgttggctat tcgcgatgga aatttatcta
      841 tgaaggaagc cagaaaattt ggaatcgcag caaccactgt acaaagattg gtgaatcaag
      901 gaaaattgat caaagttgat cgtggttatt atgtagagga tggtcatgga atagatgatt
      961 tattttggct tcagcaacgt ttttccaggg gaatcttctc tcatgaaacg actttagata
     1021 tttaccagct ttctacgaat atgccgaaat tcattcactt aacttttccc catgg
//
LOCUS       DACERL010000213         1074 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.227, whole genome shotgun sequence.
ACCESSION   DACERL010000213 DACERL010000000
VERSION     DACERL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.227"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..548
                     /locus_tag="IXF54_002950"
     CDS             <1..548
                     /locus_tag="IXF54_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1406142.1"
                     /translation="LLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQK
                     FVSHLLEVVPGQIQSTNFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAAC
                     TSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQKGSFEATIVLKEDFTT
                     GFEFSEKKKTNFKKRTRRTAK"
     gene            788..>1074
                     /locus_tag="IXF54_002951"
     CDS             788..>1074
                     /locus_tag="IXF54_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HAQ1406143.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHG"
BASE COUNT          400 a          195 c          223 g          256 t
ORIGIN      
        1 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca
       61 aatgggaaag ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag
      121 ccaatatcca aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca
      181 ctaattttgg atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc
      241 gtcatttagg aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg
      301 cagcttgtac gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac
      361 tcacagcgac acagctgaag gagatcattc aaaatagccg aacaagccaa ccagtgaaag
      421 ggttaaaagg tcagaagggt tcatttgaag caacgattgt tttaaaagaa gacttcacta
      481 caggctttga attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgccgcactg
      541 caaaataatg gagggttaat cttatgcgta ataccacaca caaacaacaa gaaatcgaaa
      601 atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg tgccagcaaa cgaagactgc
      661 agcaattgaa gaaccagcag aggattcaaa aagaaatcga attagatcga tagacattaa
      721 ccttgttaca aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg
      781 gaagtaaatg caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat
      841 gaaggaagcc agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg
      901 aaaattgatc aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt
      961 attttggctt cagcaacgat tttccagggg aatcttctct catgaaacga ctttagatat
     1021 ttaccagctt tctacgaata tgccgaaatt cattcactta acttttcccc atgg
//
LOCUS       DACERL010000214         1021 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.228, whole genome shotgun sequence.
ACCESSION   DACERL010000214 DACERL010000000
VERSION     DACERL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.228"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            22..216
                     /locus_tag="IXF54_002952"
     CDS             22..216
                     /locus_tag="IXF54_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406144.1"
                     /translation="MIFAAAAAVASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGLFGQTPQGL"
     gene            286..>1021
                     /locus_tag="IXF54_002953"
     CDS             286..>1021
                     /locus_tag="IXF54_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406145.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNE"
BASE COUNT          367 a          141 c          246 g          267 t
ORIGIN      
        1 actagtttaa aaaacaatct gatgattttt gctgctgctg cggccgttgc ttccattgtt
       61 attgggggtc ttttcttcct atttggacga agaggagctg aaaatgggaa gatgcacgta
      121 ggctatgtat tgatgggggt aggagttatt ttagcagcag cttctattgt ggtttcgtta
      181 tttggattgt ttggacaaac acctcaagga ttataagaaa gtaaaaaagt aggagtaaga
      241 agtagtcgat actttttact ccttttttat gaaaggagaa tactcatgta ctttacgatt
      301 gcaggcaata ccaaaacaga taaagagtta ataaaaaaag caacgaagga agtaggaaag
      361 cgtacttctt ttttaccgaa aacaattaaa tattctgtca ttgaaacgga agaacagcta
      421 catgtgttag ggaaagtact ggaacacgaa agtcgatctt ctgttaagtt gactctcacg
      481 ttacatgaca cagaaggaaa aatagagaaa gctggtacag aatacgtttt tggcccactg
      541 gattatcaaa tttcagaagg tttctatcct acattgatgg atttaatccg ccttgatgtg
      601 tttaaagaaa cggatgaaac tatgagtgtg gaagagtctt atcgaaacga aaaactttta
      661 caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagg gagtgaagtc
      721 gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga gtcaaaagaa
      781 aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc gatatttgag
      841 gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac aatggaggat
      901 gaagagacgg ccgaagtaga agttgatcca gttgaagcga atgaagagtc aacatggact
      961 gaaggggatt ctcaggaata tattgaacca cttatcagtg aagaagtaga aaaaaacgaa
     1021 a
//
LOCUS       DACERL010000215         1015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.229, whole genome shotgun sequence.
ACCESSION   DACERL010000215 DACERL010000000
VERSION     DACERL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.229"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(81..635)
                     /locus_tag="IXF54_002954"
     CDS             complement(81..635)
                     /locus_tag="IXF54_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1406146.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          331 a          185 c          106 g          393 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctatatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaaat ttgaacaata tactaagaac tccccttact ataaattgaa
      961 ataaattagc atatgaataa ccgtgctacc aagcttttca ttaataaaat atttt
//
LOCUS       DACERL010000216         1011 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.230, whole genome shotgun sequence.
ACCESSION   DACERL010000216 DACERL010000000
VERSION     DACERL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.230"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            312..734
                     /locus_tag="IXF54_002955"
     CDS             312..734
                     /locus_tag="IXF54_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406147.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            744..947
                     /locus_tag="IXF54_002956"
     CDS             744..947
                     /locus_tag="IXF54_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406148.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          389 a          139 c          152 g          331 t
ORIGIN      
        1 cttttaccta ctttctgcaa ctatttaaaa tacaccctaa tcatacacga ggaagccata
       61 tttttcaatg ttataccagt gtctatttag ggtatacctt atatagacaa aaactaatga
      121 tggaggaaat agtatgtgtc acttttcttt ttaaatggca atattttttg taaataaatg
      181 tcaatcaaga aaatttaaac gattatgaca ttaaaaatat attttatgaa ttttctagta
      241 tataacttga tttatctgtt aagatttatt aagaaaacgt tttcttattt ttgatttcga
      301 ggtgatagac aatgaaaaat aagtttattt tatctttagg tattttaact attggaatta
      361 gtgtaactgg aactacaatt cctacatttg ctgatagtac aaatgaacca actactgtag
      421 tagataataa ccaagaatta actcctttag ataggaacgg aatagccatt ggtaacccaa
      481 ctaacgactt aactgttact gaaaattatt taatagtttc tccattactt gctcgagcag
      541 cttctaaaac gatacgagtg acaggaacga tgatttttga taaagttggt tggccttcta
      601 gacctcctca atcacggaat tggaaagaaa aaagaaatgg ttattggtac tatggaaaat
      661 tgtatttaca aagttataaa tcagctggaa acaattggat tgcgacttat tcaggaacct
      721 taactagaaa gtagggattt tttatgccag aatttgtgaa cacaccaaat ttttatcaag
      781 atagcgatat tctaagtaaa attaatttac cgaaagaaga atcagcttta caagttcagc
      841 gaactttaca ggagacacaa aaaattcaaa aatcaatttc taatctagaa gattctgaag
      901 aaaaaagaaa aaaaagagac caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa
      961 agagtgtcta gtcgacattc tttttttcta attgaaagtg ttcataaaat c
//
LOCUS       DACERL010000217         1001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.231, whole genome shotgun sequence.
ACCESSION   DACERL010000217 DACERL010000000
VERSION     DACERL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.231"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            369..1001
                     /locus_tag="IXF54_002957"
                     /pseudo
     CDS             369..1001
                     /locus_tag="IXF54_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DACERL010000218          975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.232, whole genome shotgun sequence.
ACCESSION   DACERL010000218 DACERL010000000
VERSION     DACERL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.232"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            36..653
                     /locus_tag="IXF54_002958"
     CDS             36..653
                     /locus_tag="IXF54_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1406149.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT          345 a          128 c          197 g          305 t
ORIGIN      
        1 tgatccggat ttcagaattt taaaggagtt ttattatggc gaaaattggg tatgctcgag
       61 taagcagctt agatcaaaat ttagaccgac aattagaatt attatcagag tgtgataaaa
      121 tttttagtga taaagcaagt gggaaagata ccaatcgaga aggatttcaa gaaatgatga
      181 aattcatacg tgaaggtgat attgtcgttg tgacagaatt aaaacgactt ggacgaaata
      241 ataaagagct aacagaaacg atgaatttaa ttcagctgaa gaaagctaca ctggaagttt
      301 tgaatttgcc agcgttaaca ggtatcactg atgataattt acgtcgatta cttaataact
      361 taattatcga actatacaag taccaggccg aagaagaacg tcggtatatt cgcgaaacgc
      421 aacaacaagg aattgcactc gctaaagcaa aagggaaata caagggaggt acgctaaaat
      481 atcgtgagaa tgatccgcga cttcaattag catttaattt gtttttgact ggatctacag
      541 ataaagaagt tgaagagcta acagggatta atcgtagaac gtttagaaga tatcgaatga
      601 aacatggtat tactaatgaa gttagggagc aaaaaaataa atctttcagt tgaggctaat
      661 ataattttgc aagaaaccag gtcttaagaa agctcgtaaa gcttcacaat tctcaaaacg
      721 ttaatcgttg ttatatcaag gatttcacgg catcatccgt ttggatggtg tcttttttgg
      781 gttgaattcc ttgaaaattg ggtctatttt acagaccctt ttctatataa actgttttcg
      841 atacttggaa aaatctgttg tttttaaatt ttatttaatt ctaatgttta ctatgttttt
      901 agcagtgtta ggtaatgtaa ctgcaataaa tttgttatac aataatggat tttgattttg
      961 ggacgtaaaa cattg
//
LOCUS       DACERL010000219          975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.233, whole genome shotgun sequence.
ACCESSION   DACERL010000219 DACERL010000000
VERSION     DACERL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.233"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(67..660)
                     /locus_tag="IXF54_002959"
     CDS             complement(67..660)
                     /locus_tag="IXF54_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1406150.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            complement(776..975)
                     /locus_tag="IXF54_002960"
                     /pseudo
     CDS             complement(776..975)
                     /locus_tag="IXF54_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          257 a          190 c          151 g          377 t
ORIGIN      
        1 tttctctgtt gcaaaatttc tcgtttttgc tttaaattgc gtcggtgtca ttcaccttta
       61 ctcctctcaa taaagtggca acatatacaa agtcatttct tttggtgatt ttaaaatatc
      121 catgtatctt cttagcttat ttgtatttct actggtactt gtcatatatt cttttaatgc
      181 ttcttgtttt acctctacgc ttgctttgta tcttggattc cacatgtcac ataacgttcg
      241 ctctaaatca tagactaaaa ttttattgcc gtaaaagctt tctgtttctg tgattcccag
      301 gtcaaaattt tctttattcg tgtaatgcgg ttgaatagca tactcgtcta atttctcctt
      361 tgaaacattg tatcctttag gaaacatcat attgatcttt ttagggatat acgttcctac
      421 ttgaaaaaga tcgagcgcag tctcatgaga aaaaatccct ctggaataga tactttgagc
      481 gagatacaat tcatccacga acgcttcttt taaggtatag aaacctggtg ctaccttttc
      541 tattttgtcc ttttcggcta atcgatctaa cgtcataggc gataatcctt cacttctagc
      601 atccttcaag gatagattcc cattatgttt atcaaaaata cactggattt tgtctttcat
      661 tgcttttccc ctaaagttaa tataaaacta acataactat aacatgttag ttttatatta
      721 acaagaaaga aatttatcga tctaattcaa tttctttttg aatccgctgc tggttcttta
      781 aatacgcttc aaatgcttct tgcgtcactt gggtttcatt gggatcaagc ttggcatcaa
      841 atgtgccatt ttgagcggtt acttgggctt ttccttcaaa gtagggttct ttcttgaagt
      901 tcctaatctt ttcttgcaac tgataaatca tataaagcgt tggagtctga actctgccta
      961 aggagaagct tcctg
//
LOCUS       DACERL010000220          961 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.234, whole genome shotgun sequence.
ACCESSION   DACERL010000220 DACERL010000000
VERSION     DACERL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.234"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            36..>961
                     /locus_tag="IXF54_002961"
     CDS             36..>961
                     /locus_tag="IXF54_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406151.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLS"
BASE COUNT          343 a          173 c          185 g          260 t
ORIGIN      
        1 agttccgacg aaagaattat aaggagtgag tacaaatgac ctataaccat cttacaccaa
       61 ctgaactcgt aatgatagaa gcttatttca atcaatcaca acctgtttca aaagtagcca
      121 atctgttaca acgttctcga caaactattt ataaggtcta tcgttttctt aaatctggtg
      181 gaagcgcaat gagctactat aaacaatata agaaaaacaa acgtaactgc ggaagacatc
      241 cgattgtttt acctgatgag caacaggaat acattcaaaa aaaggttgtt caagggtggg
      301 ctcctgatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta cgcacactgt
      361 atcgaatgtt taaagataaa acctttgaca ctcacgattt accaatgaag ggaaaaagaa
      421 aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt tcgccggtca atccgagaca
      481 gaaagacaga ctatacccag tttgatcatg aatttggtca cctggaaggt gacactatcg
      541 tgggtggaaa acataaaagc gcggtcatca cattagttga acggctatct aaagtcatca
      601 ttaccttgaa acccgaaggc agacaggcta aagacattga aaataggctg aatcaatggt
      661 ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt tgattgtgga aaagaattct
      721 caaattggaa aaagattagt aataccaacg atatttcaat ttattttgcg gatccaggaa
      781 caccttctca acgaggattg aatgaacatt caaatggctt attacgtaaa gatggtttgt
      841 ataaagctat ggattttaat ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac
      901 gaaatcatat tcctagaaaa tcattacact atcggacacc aatggaagta tttttgagtt
      961 a
//
LOCUS       DACERL010000221          936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.235, whole genome shotgun sequence.
ACCESSION   DACERL010000221 DACERL010000000
VERSION     DACERL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.235"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            125..709
                     /locus_tag="IXF54_002962"
     CDS             125..709
                     /locus_tag="IXF54_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1406152.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          303 a          131 c          188 g          314 t
ORIGIN      
        1 tttttgcttt atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc
       61 tatgttcagt taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat
      121 ttttttgaaa ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga
      181 agacagccta aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa
      241 aagtaatcga ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt
      301 cgtttggcgc ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg
      361 actgaatgag cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc
      421 aagttctaca ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa
      481 tttgatcctg gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg
      541 tggtcgtcct gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag
      601 cggtacacct attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg
      661 ttatttggca aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt
      721 taatgctgaa aatttttgcc atttatctag aaaagtgaca atagtatgag aattttttgt
      781 aagtgtctac tgataactta ttctcaaatg ttgtaaggat tttattcatt tgttaatatt
      841 aaagaacgga actccaaaaa agtccgtact ctaatttgca gtctctcact cttttagagt
      901 gagagttttt attagatttt caaattaatt agtaaa
//
LOCUS       DACERL010000222          930 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.236, whole genome shotgun sequence.
ACCESSION   DACERL010000222 DACERL010000000
VERSION     DACERL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.236"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>930)
                     /locus_tag="IXF54_002963"
     rRNA            complement(<1..>930)
                     /locus_tag="IXF54_002963"
                     /product="16S ribosomal RNA"
BASE COUNT          196 a          295 c          207 g          232 t
ORIGIN      
        1 ggcaactaac aataagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
       61 gctgacgaca accatgcacc acctgtcact ttgcccccga aggggaagct ctatctctag
      121 agtggtcaaa ggatgtcaag acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca
      181 tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag tttcaacctt gcggtcgtac
      241 tccccaggcg gagtgcttaa tgcgttagct gcagcactga agggcggaaa ccctccaaca
      301 cttagcactc atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac
      361 gctttcgagc ctcagcgtca gttacagacc agagagccgc cttcgccact ggtgttcctc
      421 catatatcta cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag
      481 tctcccagtt tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga
      541 aaccgcctgc gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta
      601 ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa
      661 cagttactct catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt
      721 cactcacgcg gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc
      781 ctcccgtagg agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg
      841 gctatgcatc gtggccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc
      901 catccatcag cgacacccga aagcgccttt
//
LOCUS       DACERL010000223          930 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.237, whole genome shotgun sequence.
ACCESSION   DACERL010000223 DACERL010000000
VERSION     DACERL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.237"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            149..721
                     /locus_tag="IXF54_002964"
     CDS             149..721
                     /locus_tag="IXF54_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1406153.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT          332 a          138 c          203 g          257 t
ORIGIN      
        1 gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa
       61 ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt
      121 tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct
      181 tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat
      241 aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa taccaattgg
      301 atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc cggtaatgtt
      361 tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt gccatggaga
      421 tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta ttttgaagca
      481 tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca gcacatctgg
      541 acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa tctgcaataa
      601 aagggtaata tcaattcaat atcctgcatc aggagggcaa gaaccggaaa gcagaatgat
      661 agagcctata tcactggggt tttatgaaca gaactggtac ttaatcggtt ttgcaggcta
      721 agaaatgaat ataggaattt tcgtgtggat cggattaaaa gcatttgcat agaagaagag
      781 ctctgtcaaa gccatgatgg ctctttggaa caaatattaa aacaaatgct ttcatataaa
      841 aaactatatg ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat
      901 atctttctca aaataaatat aaaagccctt
//
LOCUS       DACERL010000224          913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.238, whole genome shotgun sequence.
ACCESSION   DACERL010000224 DACERL010000000
VERSION     DACERL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.238"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>913
                     /locus_tag="IXF54_002965"
     CDS             <1..>913
                     /locus_tag="IXF54_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1406154.1"
                     /translation="GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGA
                     FDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQS
                     KMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAE
                     AIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAM
                     FAEKYTYEETTILTQVQQLQLKAIGKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKG
                     EMVTVDLQVLEKETKPPQPYTEGTLITA"
BASE COUNT          328 a          178 c          202 g          205 t
ORIGIN      
        1 caggaagttt ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattac
       61 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
      121 ctcaaaatgg cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag
      181 catttgaaga ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggtacgatcc
      241 accaagttga aaccgagaaa aaaagtgcgg caagtccacg tcttttttct ctatccagcc
      301 ttcaatctaa aatgaatcag ctcatgaaag ctagtgccaa agatactttg gaagccatgc
      361 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
      421 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
      481 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
      541 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
      601 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
      661 cggaaaagta tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa
      721 aggcaatcgg aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg
      781 acggaaaaga gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga
      841 cggtggatct acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta
      901 cgttgatcac tgc
//
LOCUS       DACERL010000225          909 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.239, whole genome shotgun sequence.
ACCESSION   DACERL010000225 DACERL010000000
VERSION     DACERL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.239"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..878
                     /locus_tag="IXF54_002966"
     CDS             <1..878
                     /locus_tag="IXF54_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1406155.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          291 a          202 c          184 g          232 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaat
//
LOCUS       DACERL010000226          890 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.240, whole genome shotgun sequence.
ACCESSION   DACERL010000226 DACERL010000000
VERSION     DACERL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.240"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..774
                     /locus_tag="IXF54_002967"
     CDS             <1..774
                     /locus_tag="IXF54_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1406156.1"
                     /translation="HFLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAE
                     LPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIR
                     ALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAK
                     YAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEV
                     PKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          318 a          173 c          157 g          242 t
ORIGIN      
        1 cattttcttg aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa
       61 tcgacgagtc tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat
      121 gaacttgctg agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc
      181 cgtttcaagc aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac
      241 cgcctaggtg ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa
      301 atacgagcac ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt
      361 gtcattccag aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc
      421 ggtttaatcg ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa
      481 tacgctggat taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct
      541 ctgacaaaac aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta
      601 aaaaattatc ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag
      661 catcaacaca aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg
      721 ctactacgta aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc
      781 gttattgacg caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt
      841 tcgtgcgctt caaaacatat tactcaataa agaaaatctt aaatttcatc
//
LOCUS       DACERL010000227          882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.241, whole genome shotgun sequence.
ACCESSION   DACERL010000227 DACERL010000000
VERSION     DACERL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.241"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..479)
                     /locus_tag="IXF54_002968"
     CDS             complement(<1..479)
                     /locus_tag="IXF54_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1406157.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEV"
     gene            complement(492..>882)
                     /locus_tag="IXF54_002969"
     CDS             complement(492..>882)
                     /locus_tag="IXF54_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1406158.1"
                     /translation="KNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSDKEGHFEI
                     TGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAIPLTGSNG
                     FQTYVLISCLLLGAGGLSAVVYFKKKA"
BASE COUNT          245 a          182 c          125 g          330 t
ORIGIN      
        1 ttaacttctt caattcgata aatttgatag gcatgttgtt cttcatttac cttaataaag
       61 aatttttgcc ctttttttac tgaaggcaaa tcatcgaaaa gattatcttt ataaagtcca
      121 ctgtgccccg cgattaaagg atttttaccg tttcctcctg taatatcgcc agttccttcc
      181 gtaatcccta caccattctc taaaattttg tcggaagttc cgtcataaat tggtagtttt
      241 aaagaaatac ttggaattgt aagaacacca acaggatcgc ctaactctat tttcgcaaca
      301 tccaaagaag aatctgtttt ttcaagattt tcaatggcta aaattctttg ctgttgttga
      361 aatttcgcta catttgtttc cacaactgga attaaataga ctgctactgc cgacagcaac
      421 caaaaaaata tcatcagttt gtgcatgact cttctttttg ggggcttacg aataatcatt
      481 agctgatcct tctaggcttt cttcttaaag taaacaactg cagaaagtcc ccctgctcct
      541 agcaacaagc aagagatcaa tacatacgtt tggaaaccat tactacctgt taatggaata
      601 gctgcttttt gattattttt aacagtgatt cgattttcct ctttaaatga gtttttgttt
      661 actttaaatg caatatcttc tgttaatttt tgatagtttt caggcgcttt tgtttcttcc
      721 aatgaatatt caccttcagt taaaccagtg atttcaaagt gcccttcttt atctgatttt
      781 agaatcgctg catttgctac attgtctacc catactgctt ctttgacacc gtctttatcc
      841 gtatcaacta atccaccata atattttcct tctgcgtttt ta
//
LOCUS       DACERL010000228          876 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.242, whole genome shotgun sequence.
ACCESSION   DACERL010000228 DACERL010000000
VERSION     DACERL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.242"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..434)
                     /locus_tag="IXF54_002970"
     CDS             complement(<1..434)
                     /locus_tag="IXF54_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1406159.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IXF54_002971"
     CDS             complement(451..855)
                     /locus_tag="IXF54_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1406160.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DACERL010000229          845 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.243, whole genome shotgun sequence.
ACCESSION   DACERL010000229 DACERL010000000
VERSION     DACERL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.243"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..780)
                     /locus_tag="IXF54_002972"
     CDS             complement(<1..780)
                     /locus_tag="IXF54_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1406161.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTK"
     gene            complement(796..>845)
                     /locus_tag="IXF54_002973"
     CDS             complement(796..>845)
                     /locus_tag="IXF54_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406162.1"
                     /translation="DKSIYHWDFYALLGF"
BASE COUNT          249 a          171 c          123 g          302 t
ORIGIN      
        1 ttttgtatat tcaattttga aaacatttcc gcaaagttca gacggacctc gatgtgttga
       61 tgaataaaat ttattagtaa taacttctat aaggttatca atccctatat tactttttgc
      121 acttccatga taaagaggga acagagaaca attctgaaat cttatgcttt cctcttgttc
      181 gagttccaat gcttctaatg atttaccgga catatatttc tctaaaaggt catcgtttcc
      241 ctctattacc gtatcccatt gttcagattc ggtaaagttc gtcacacaca tattaggata
      301 cagttctacc ttctgtttga ttacaatttc ggcagaaagt ttctctttaa tatcctgata
      361 aaccgttgat aaatcaattc cattttggtc aatcttattg ataaaaaaga ttgtgggaat
      421 ccccattttc ctaagtgcat gaaataatat acgagtttgt gcttgtacgc catcttttgc
      481 agaaatcagt agaattgccc catctaaaac tgataatgaa cgatatactt ctgctaagaa
      541 atccatatgt cctggcgtgt ctatgatgtt caccttcgta ttttcccact gaaaagaggt
      601 tattcctgtc tgaattgtaa ttcctctctg acgttctaaa agcgtattat ccgtcctcgt
      661 tgtacctttg tccacgcttc ctaattctgt aatcgctcca ctgttatata ataagctttc
      721 tgttaaggta gtttttcctg catcaacatg agctaaaact ccaatattaa taattttcat
      781 gtgattttcc tccattcaaa aacccaaaag ggcataaaaa tcccagtgat aaatactttt
      841 atcac
//
LOCUS       DACERL010000230          822 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.244, whole genome shotgun sequence.
ACCESSION   DACERL010000230 DACERL010000000
VERSION     DACERL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.244"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            22..>822
                     /locus_tag="IXF54_002974"
     CDS             22..>822
                     /locus_tag="IXF54_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1406163.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          185 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa ac
//
LOCUS       DACERL010000231          811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.245, whole genome shotgun sequence.
ACCESSION   DACERL010000231 DACERL010000000
VERSION     DACERL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.245"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IXF54_002975"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IXF54_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1406164.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACERL010000232          804 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.246, whole genome shotgun sequence.
ACCESSION   DACERL010000232 DACERL010000000
VERSION     DACERL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.246"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(330..530)
                     /locus_tag="IXF54_002976"
     CDS             complement(330..530)
                     /locus_tag="IXF54_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1406165.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          289 a          127 c          133 g          255 t
ORIGIN      
        1 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
       61 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
      121 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
      181 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
      241 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
      301 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
      361 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
      421 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
      481 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
      541 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
      601 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
      661 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
      721 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
      781 tagatccttt attctatgct ttat
//
LOCUS       DACERL010000233          748 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.249, whole genome shotgun sequence.
ACCESSION   DACERL010000233 DACERL010000000
VERSION     DACERL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.249"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>748
                     /locus_tag="IXF54_002977"
     CDS             <1..>748
                     /locus_tag="IXF54_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406166.1"
                     /translation="LEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGIVI
                     PSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTDPEQYRYKPNSDLENEKRI
                     SDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKRISDLPKRNQEVQTDEDSIVL
                     PNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDNDQSFVHQSPAGTVQMKPSRLP
                     ENGNLTTVSYNLSESGETGNASVQNVVTREA"
BASE COUNT          321 a          119 c          144 g          164 t
ORIGIN      
        1 cactagaaaa acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg
       61 gtgaaggaca agttaatgca gcagtaaaag ctaatacaac aaccgaaaaa gaaaatcaag
      121 aaggtatagt gattccttca gtcaaagatc accaagaagc gttgcactct aatgaaaatt
      181 cgattgaaga gaaaaattcc atagaagaaa gaaattcaga agaaggaaaa aatggacagt
      241 taccaaaaac tgatccagaa caatatcgct ataaacctaa cagtgacctt gaaaatgaga
      301 agcgaatttc agatttatca aaagtaaatc aagaagacaa agcagaaaat gagggacagt
      361 taccaaaaac tgatccagaa caatatcggt atagatctgt taactccgaa aatgaaaaac
      421 gcatttcaga tttaccaaaa cgaaatcaag aagtacaaac ggatgaggat tccatcgttc
      481 tgccaaatga taatggaatc gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta
      541 aggcacaaaa tttaccaatg aaagatagtc gtttgaatca attcaaggat aatgatcaaa
      601 gttttgttca tcaatcacct gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa
      661 atggaaatct gacaactgtt tcttacaatt tatctgaaag tggtgagaca gggaatgcgt
      721 ccgttcaaaa tgttgtgaca agagaagc
//
LOCUS       DACERL010000234          746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.250, whole genome shotgun sequence.
ACCESSION   DACERL010000234 DACERL010000000
VERSION     DACERL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.250"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>746
                     /locus_tag="IXF54_002978"
     CDS             <1..>746
                     /locus_tag="IXF54_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406167.1"
                     /translation="NKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIACS
                     VRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEFGH
                     LEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFKSI
                     TFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNKVS
                     ETFIQSVASKRNHIPRKSLHYRTPLEVFLS"
BASE COUNT          260 a          133 c          150 g          203 t
ORIGIN      
        1 aacaaacgta actgcggaag acgtccgatt gtgttaccag atgggcaaca agaatacatt
       61 caaaaaaagg tggctcaagg gtggactcca gatgtcctta ttggtcgagc agagtttcct
      121 attgcttgtt cggtacgtac actatatcgg atgtttaaag ataaaacctt tgaccctcac
      181 gatttaccaa tgaaagggaa aagaaaacca aacggacata aagaaaaaag aggaaaacaa
      241 gcttttcgcc gttcgattca ggatagaaca actgattata tacagttcaa tcatgagttt
      301 ggtcacctag aaggtgacac cattgtcggt ggaaaacata aaagtgcggt gatcacgtta
      361 gttgagcgat tatctaaagt gatcatcacc ttgaaacccg ctggtaggca agctatagat
      421 attgagaata gactgaatca atggtttcaa tctgtaccga aacacttgtt taaatccatc
      481 acatttgatt gtggaaaaga gttctcgaat tggaaaaaga ttagtaatac caatgatatt
      541 tcgatttatt tcgctgatcc aggaacacct tctcaacgag gactaaatga acattcgaat
      601 ggcttattac gtaaagatgg cttatataaa gctatggatt ttaataaggt atccgaaacc
      661 tttatccaat cggtggcatc aaaacgaaat catattccta gaaaatcatt acactatcgc
      721 acaccattgg aagtattttt gagtta
//
LOCUS       DACERL010000235          746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.251, whole genome shotgun sequence.
ACCESSION   DACERL010000235 DACERL010000000
VERSION     DACERL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.251"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>746
                     /locus_tag="IXF54_002979"
     rRNA            <1..>746
                     /locus_tag="IXF54_002979"
                     /product="23S ribosomal RNA"
BASE COUNT          227 a          152 c          221 g          146 t
ORIGIN      
        1 tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga agacacctag gaggatcctg
       61 agtacggcgg aacacgagaa attccgtcgg aatccgggag gaccatctcc caaggctaaa
      121 tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga
      181 aggggagtga aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg
      241 tgatggcgtg ccttttgtag aatgaaccgg cgagttacga ttgcatgcga ggttaagttg
      301 aagagacgga gccgcagcga aagcgagtct gaatagggcg tttgagtatg tagtcgtaga
      361 cccgaaacca tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg cactggagga
      421 ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa
      481 cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcctc ggaattgaga
      541 atgatggagg tagagcactg tttggactag gggcccatct cgggttaccg aattcagata
      601 aactccgaat gccattcatt catatccggg agtcagactg tgagtgataa gatccatagt
      661 cgaaagggaa acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag
      721 gatgtggggt tgcacagaca actagg
//
LOCUS       DACERL010000236          742 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.252, whole genome shotgun sequence.
ACCESSION   DACERL010000236 DACERL010000000
VERSION     DACERL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.252"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            74..583
                     /locus_tag="IXF54_002980"
     CDS             74..583
                     /locus_tag="IXF54_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1406168.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT          284 a           99 c          160 g          199 t
ORIGIN      
        1 tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa gactaaatac
       61 ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg cgattgattt
      121 gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga ctaaagaaga
      181 agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag aaaataatgt
      241 gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc tgattctctt
      301 ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa gcaaggaaat
      361 caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta tgaccactga
      421 tgatatttta gacgtttttg ctgaggcaga aatctatcca tcaaaaggcg aaatcggggc
      481 atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat atatgcgtaa
      541 ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt tcatgagaaa
      601 tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa tattatctta
      661 atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag ataagatgct
      721 ttttgaagag atagagaatc ga
//
LOCUS       DACERL010000237          733 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.253, whole genome shotgun sequence.
ACCESSION   DACERL010000237 DACERL010000000
VERSION     DACERL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.253"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            25..>733
                     /locus_tag="IXF54_002981"
     CDS             25..>733
                     /locus_tag="IXF54_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406169.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          259 a          126 c          139 g          209 t
ORIGIN      
        1 aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga tgaacttgta
       61 ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca ttgcttgaat
      121 cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa atcagcctta
      181 gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc gcttgtttta
      241 cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg
      301 attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc
      361 aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga
      421 catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat
      481 tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa
      541 cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa
      601 ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta
      661 ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa
      721 cagatcagta atg
//
LOCUS       DACERL010000238          721 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.254, whole genome shotgun sequence.
ACCESSION   DACERL010000238 DACERL010000000
VERSION     DACERL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.254"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>721
                     /locus_tag="IXF54_002982"
     CDS             <1..>721
                     /locus_tag="IXF54_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406170.1"
                     /translation="LKAAGRKMMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEK
                     ENTRKPYTEVENGVSYVVPKGKRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLL
                     TRKSDLRNSLTKVVKEPVRYDRHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTRE
                     KLDELTQYVLTKEPEYQEYIQISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGN
                     AILTELKRQDSQIKPLIESVDQ"
BASE COUNT          302 a          107 c          152 g          160 t
ORIGIN      
        1 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagagca actgaacgaa
       61 acaggctact ggatggcaac gattcaccgt aacactaaac atatccatat tcattttgca
      121 gttgttgaaa aagaaaatac gcgtaaacct tacactgagg ttgaaaatgg tgtgagttat
      181 gttgtaccga aaggaaagag aaggcaagca acattagacg caatgaaaac caattatgtg
      241 catgagttag aacgtttctc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
      301 ttattgacac gaaaaagtga tttgcgtaac agcttaacaa aagtggtaaa agagccagta
      361 cgttatgatc gacacgcaat gaacttatta aatgaagtat atagaaactt acctgaaaaa
      421 cgttcggaat ggaattatgg ggatgaaaat cgaaccaaac tttcaccata cacaagagag
      481 aaactcgacg agttaaccca atatgtgtta acaaaagaac ctgaatacca agaatatata
      541 cagatttcaa aagcattgaa agaagaagca aaaaatcttt atggggaaag caagagagaa
      601 tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatc
      661 ctaacagagc tgaagagaca ggatagtcaa ataaaaccat taatagaaag tgttgatcag
      721 a
//
LOCUS       DACERL010000239          686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.255, whole genome shotgun sequence.
ACCESSION   DACERL010000239 DACERL010000000
VERSION     DACERL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.255"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(24..374)
                     /locus_tag="IXF54_002983"
     CDS             complement(24..374)
                     /locus_tag="IXF54_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1406171.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(368..631)
                     /locus_tag="IXF54_002984"
     CDS             complement(368..631)
                     /locus_tag="IXF54_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1406172.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          194 a          135 c           82 g          275 t
ORIGIN      
        1 ttgatcatgc tgctgtttct attttatttg cttacttttt gaaaaactgt tctaactttc
       61 atcaataact cctcaactaa actatcatgc gctttttcca aaaaaataca ctctctggct
      121 tcataatcaa ttgtcgttag ttgatctaat aatacttttc ctgttgtctt catttcatat
      181 tctgagttta tatttacata tagcggatag tttctagttg tactagaaat tggagcaact
      241 gtaacaacgt tactataatc cgcaataata tcatgactta aaacaatata aggcctttta
      301 cctgtctgtt cgtgtccaga tcgaggtgct gtattcaata atagaatatc gccttgtcta
      361 ggttttctca ccatttttca ttacctactg cttcacctaa attttgaatc gtcacatgat
      421 ttctttcatc agtgtaattt tcaaataact gttcaattgt taattcattt tctatattag
      481 ccgcttcgat aattagccgc ttctgaccgt ttacttcttc ttgtattatc ttcactttgt
      541 cttctgtatg aattccaaga tattccataa tatactttgg caatctaagg ccattactat
      601 ttccccattt ttttataggt aacatttcca tagtagacac ctctttctgt atatacatag
      661 tataacatac ataagaaaaa acgtat
//
LOCUS       DACERL010000240          683 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.256, whole genome shotgun sequence.
ACCESSION   DACERL010000240 DACERL010000000
VERSION     DACERL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.256"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>683
                     /locus_tag="IXF54_002985"
     rRNA            <1..>683
                     /locus_tag="IXF54_002985"
                     /product="23S ribosomal RNA"
BASE COUNT          161 a          159 c          220 g          143 t
ORIGIN      
        1 catgtacagg ataggtagga gccgtagaaa tcggaacgct agtttcgatg gaggcgctgg
       61 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
      121 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      181 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      241 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      301 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      361 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      421 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      481 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      541 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      601 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      661 cccacctcaa gatgagattt ccc
//
LOCUS       DACERL010000241          653 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.257, whole genome shotgun sequence.
ACCESSION   DACERL010000241 DACERL010000000
VERSION     DACERL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.257"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            208..>653
                     /locus_tag="IXF54_002986"
     CDS             208..>653
                     /locus_tag="IXF54_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1406173.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLL"
BASE COUNT          227 a           90 c          124 g          212 t
ORIGIN      
        1 tttctcaatt atcaatttat ccaagatatc aacgattgtt cattcatttt ctaaacattc
       61 aatgtgcttc catgccatta gaggtgatta aaaagctctt gatcagtcca atttttatgg
      121 ttagtttggt tcttcttaca ggattggtaa cgtgggtatt aggaaaaaaa gaatgaaaag
      181 cttacgatcg tttaaaaaaa atattagatg gagggcatta tggaaaataa aattcattgc
      241 actacgggaa gagtaattac tttagtgttg tttttgttcc ttcttctttt tgcgagggga
      301 actgcagtcc aggccttaga aaaagttgat ccgagtcaaa tcaaagaaaa taataatctg
      361 gtgctttatc atgatagtga ttcattgaga aatatcgaag taactgggat caatggattt
      421 aagatactag atgagtattc aactaaaaaa caaaaagtac ttgcttggcc aaaagtcaaa
      481 gatttgaaaa gtacgtcaaa aatcgttgcc cattatcaaa aagtaggtac ttatctagga
      541 aaagaaattg attgtgacgt gactttttct aattttgtag cgcacgctgg ggttaaccaa
      601 gacggattag ctaaaacaat taatggcaaa aagtattata attatttgct ctt
//
LOCUS       DACERL010000242          651 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.258, whole genome shotgun sequence.
ACCESSION   DACERL010000242 DACERL010000000
VERSION     DACERL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.258"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            44..>651
                     /locus_tag="IXF54_002987"
     CDS             44..>651
                     /locus_tag="IXF54_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ1406174.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          216 a          115 c          130 g          190 t
ORIGIN      
        1 ttaaatcacg acaaaataaa caactgagag gacgatatgc tatatgaatg attctatcaa
       61 aaaaatgctg cgattaatag agaaagattt gatgattaca gaggtctctt acgagacctt
      121 tcagaagaaa aagacgttga tcgtcgatgc tgttttctca cctgctcccc atacctgtag
      181 aaattgtggt tctactgtgg tagatggaaa cgggaaagta attgtagtga aaaatgggaa
      241 gaaggaaacg attgtccgct ttgaacaata caatcacatg cctttggtta tgcgcttaaa
      301 aaagcagcgc tatacctgta aaaactgtcg aacccattgg acggctcaaa gttattttgt
      361 tcaacccaga cattcaatcg cgaatcatgt cagatataaa attgcttctt tactgactga
      421 aaaagtatct ttatctttta ttgcgaaaaa ttgtcaggta tctttgacca ccgttattcg
      481 tacgttgaaa gagtttaaaa gctacttacc aaagcaatct aagaggattc tccccagagt
      541 attgatggtt gatgaatttc gttcgcacgc ttccatagaa gataaaatga gctttatttg
      601 cgcagatggc gaaacaggac aattaattga tgtcttgcct acgcgtaaat t
//
LOCUS       DACERL010000243          643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.259, whole genome shotgun sequence.
ACCESSION   DACERL010000243 DACERL010000000
VERSION     DACERL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.259"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            441..>643
                     /locus_tag="IXF54_002988"
     CDS             441..>643
                     /locus_tag="IXF54_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1406175.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKK"
BASE COUNT          212 a          131 c          136 g          164 t
ORIGIN      
        1 gaagagttgt agtcctaaat gaggattgca gctctttttg ttttacatag aaacgggaac
       61 aggaaaaggc ctgtttagga aggcacaagc gccttctcaa ttgaaaaacc tttctctcac
      121 tccaaccgcc accgccgttc gagaagaaag gaagtgaacg ttgcacgttc actaaaataa
      181 ggggtcttgc aaagcaagac aaaataggaa aaacttcctc tcaccaaaag gttcgttcct
      241 gttcgctatc tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa
      301 gcgacaagga caagggtaaa tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga
      361 ttcattctgg ccaacaaatc aataaagaaa gaactaacaa aaggaagtaa accctttcct
      421 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
      481 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
      541 aatgtatcaa aagctatcgt ttgtatcaat gtcaaacatg cgggaaaaaa tataccttgt
      601 cttatggcga ttatgttttg attgatgaga aaggaaagaa aca
//
LOCUS       DACERL010000244          642 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.260, whole genome shotgun sequence.
ACCESSION   DACERL010000244 DACERL010000000
VERSION     DACERL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.260"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(254..589)
                     /locus_tag="IXF54_002989"
     CDS             complement(254..589)
                     /locus_tag="IXF54_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406176.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
BASE COUNT          176 a          142 c           88 g          236 t
ORIGIN      
        1 gcttttgccg ctttgtctgt gactacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acgagattgt ctctgctttt ttcaacaaca actccaatcg ttgaataagc catttcttct
      121 tcctcctttc ttttgatata atgtagacgg aaatcgcacc actcaatgaa ttggcggaaa
      181 acaattcagt gaaaaaaaag agtcctggac aatcacgttc aggactcttt tacttctttt
      241 attcacttaa caactaacat tctgtgagtg aaatagactc ttcaatgctt ttacacggga
      301 agttattgat cccctcttca ttcatttccc aagtagtata ataaggcgct aacttgccct
      361 catattcttg aatgataaaa tatttttcac ccttcacatg attacaacag tacacaccag
      421 tagctgggta ctcaacgact aaaaattgat ttggatcaaa ctgttcgttt atcaacattt
      481 ctcttatctg aaacaaatgc tctttttcta aacaatcggg aagttgttcg attcgttctt
      541 ctcttaatga tccttctata actttccatt gatctaaata ttgtttcatt gctattcctc
      601 ctactgtctt tactgtcttt actgtctttc ttgttccatt tg
//
LOCUS       DACERL010000245          630 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.261, whole genome shotgun sequence.
ACCESSION   DACERL010000245 DACERL010000000
VERSION     DACERL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.261"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(260..595)
                     /locus_tag="IXF54_002990"
                     /pseudo
     CDS             complement(260..595)
                     /locus_tag="IXF54_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          172 a          135 c           87 g          236 t
ORIGIN      
        1 gcttttgcag ctttgtctgt gactacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acgagattgt ctctgctttt ttcaacaaca actccaatag ttgaataagc catttctgct
      121 tcctcccttc tctttctttt gatataatgt agtcggaaat cgcaccactc aatgaattgg
      181 cggaaaacaa ttcagtgaaa aaaaagagtc ctggacaatc acgttcagga ctcttttact
      241 tcttttattc acttaataac taacactctg tgagtgaaat agactctttt atgcttttac
      301 atggaaagtt attgattccc tcttcattca tttcccaagt agtataataa ggtgttaact
      361 tggcctcata ttcttgaatg ataaaatatt tttcaccgtt cacatgatta caacaataca
      421 caccagtagc tgggtactca acgactaaaa attaatttgg atcaatctgt tcgcttctca
      481 acatttctct tatctgaaac aaatgctctt tttccaaaca atcgggaagt tgttcgattc
      541 gttctttttt tagtgatcct tctatgactt tccattgatt caaatatcgt ttcatttctc
      601 ttcctcctac tgtctttctt gttccatttg
//
LOCUS       DACERL010000246          619 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.262, whole genome shotgun sequence.
ACCESSION   DACERL010000246 DACERL010000000
VERSION     DACERL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.262"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            283..394
                     /locus_tag="IXF54_002991"
                     /pseudo
     CDS             283..394
                     /locus_tag="IXF54_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            415..498
                     /locus_tag="IXF54_002992"
     CDS             415..498
                     /locus_tag="IXF54_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ1406177.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
BASE COUNT          209 a           76 c          116 g          218 t
ORIGIN      
        1 ggtcccgagc gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt
       61 tattttgtac atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt
      121 tttcaaccgt tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg
      181 gttattgcag gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg
      241 agtattgtcc gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga
      301 cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg
      361 aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg
      421 gtattccaaa tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt
      481 gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt
      541 tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg
      601 taatcacttc aggagtgat
//
LOCUS       DACERL010000247          617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.263, whole genome shotgun sequence.
ACCESSION   DACERL010000247 DACERL010000000
VERSION     DACERL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.263"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..387)
                     /locus_tag="IXF54_002993"
     CDS             complement(<1..387)
                     /locus_tag="IXF54_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406178.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDI"
BASE COUNT          164 a          116 c          112 g          225 t
ORIGIN      
        1 gatatcgtag acaataaact ttgcgccatt gataccttct tgctgggcta cttgactgcc
       61 ttgattgttg atttgattcg aagaaataat tttttgaata tgaatggctt gcttgatcac
      121 ttgcgtatct tctacgtctt gcggcttttc tgaagtctct gaagaacttt ctgacgttgg
      181 attcgttgat tcagtaaagc ttatatttga atctgccgtt tcttctgtag tattctccga
      241 actgtccgtt tcgcttggtg cagtaattga attatctatt gtttgagtgg tactactatc
      301 actgctgtca gcgaaagaca gttgcgaata agctacagta gaaaagagag ctgctacaca
      361 aacaaacagc cattttctct tattcattcc gatctcctcc ttattttttt ataaataaaa
      421 ataataggaa cgcaaaaaaa agcgaatagc agtgctaatc gccaatccaa tgaaattgat
      481 ttcctatttt ttgctttttt ctgtagtttt aaatcttctt tactgaagga aactcgttca
      541 ccatacacta aaaagcggtg tgagttgaca ccttttggtg tgcatgtcat taacgtgatc
      601 ctatcttggt ctttttc
//
LOCUS       DACERL010000248          603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.264, whole genome shotgun sequence.
ACCESSION   DACERL010000248 DACERL010000000
VERSION     DACERL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.264"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>603
                     /locus_tag="IXF54_002994"
     CDS             <1..>603
                     /locus_tag="IXF54_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406179.1"
                     /translation="DDNGKEIPVQISDEGLAKYVYTSGYRRYSSKPGIILAGESSLAV
                     AYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILEL
                     RIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNS
                     LLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFG"
BASE COUNT          172 a           91 c          136 g          204 t
ORIGIN      
        1 gatgataatg ggaaagaaat tcctgttcaa attagtgacg aaggcttagc aaaatatgtc
       61 tatacttcag ggtatcgtcg atacagtagt aaaccaggta ttatcttagc tggtgaatct
      121 tctttggcgg tggcctattt atttatccta tttacgatcg ttacttgcat tattgagctg
      181 gtatttaaaa aattctattt agtgattgcc gcgtcaacgg actttgagac aggccaacgg
      241 atgaaaagtg cgatagaaga tatttctcaa tccttcttgt tattggcgtt tacgatttta
      301 gaattacgaa tctatacatt aatgctttca ggtatcggtg atttgcatgc ggcaggaaag
      361 atcaatggat tcttgtatgt tgttgcttta attgtgctaa ctattgcgct attcaaaggc
      421 tctcaatcgg ttacgaagat tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct
      481 cttcttagca tgtttgcctt aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac
      541 cttgcaagtg ctggtaagag tggtttaagc catttaaatg atattcgtaa aaatggattt
      601 ggc
//
LOCUS       DACERL010000249          594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.265, whole genome shotgun sequence.
ACCESSION   DACERL010000249 DACERL010000000
VERSION     DACERL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.265"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            58..261
                     /locus_tag="IXF54_002995"
     CDS             58..261
                     /locus_tag="IXF54_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1406180.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            258..554
                     /locus_tag="IXF54_002996"
     CDS             258..554
                     /locus_tag="IXF54_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406181.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWTAKSKENLAVARLVDMELINDK"
BASE COUNT          225 a           89 c          134 g          146 t
ORIGIN      
        1 ttgaaagaat ttcgatcaag tataaacgac actttcagat tagggagttg attgacaatg
       61 aacaccagac accgcagagt agcaaaacta agaaaacagg aactgaatgt actaaagaca
      121 aagtttgaaa aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagttt
      181 gttgctgatg caagtgatgc tattcgtaag tttgggattt cgatattaaa tgatgatcgt
      241 aaatgggagg aaatgagatg aaactaaaag acggatttta cgctagtagt cacggcatcg
      301 gcggtttaat gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaagtaa
      361 aagttggtga catggttcgc tgtgaagcag aagggttcat ctatccattt cgtggatatg
      421 tagagcatct ctataatcac tcagcgatca ttcgtattga aaacacgatg gaatgtgata
      481 aatggacagc taaaagcaaa gagaatttag ctgtagcgag attggtggat atggaactaa
      541 tcaatgacaa ataaaaaagc cggatcgctc cgactgttct aataaattcc acaa
//
LOCUS       DACERL010000250          588 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.266, whole genome shotgun sequence.
ACCESSION   DACERL010000250 DACERL010000000
VERSION     DACERL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.266"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>588
                     /locus_tag="IXF54_002997"
     CDS             <1..>588
                     /locus_tag="IXF54_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406182.1"
                     /translation="KEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVEKINNFTKT
                     NQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKH
                     EQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAI
                     EKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLA"
BASE COUNT          287 a           82 c          120 g           99 t
ORIGIN      
        1 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaatcaa
       61 agagcggtac gaaaagaaac gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa
      121 ttttactaag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
      181 tattaaagaa cgaaaagaag agttctaccg cggtttacgt acagataagc taaagattag
      241 caaagaatta aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga
      301 aaaacacgaa cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta
      361 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
      421 gcgtgaacaa ttagaaataa gaaaacggga acaggatctt gcggaacaaa aaatggcgat
      481 agaaaaacaa cggttagagc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
      541 agaacaaatg gatacattgc gtcaagcaca gttgaattat ctagcaca
//
LOCUS       DACERL010000251          581 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.267, whole genome shotgun sequence.
ACCESSION   DACERL010000251 DACERL010000000
VERSION     DACERL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.267"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            67..>581
                     /locus_tag="IXF54_002998"
     CDS             67..>581
                     /locus_tag="IXF54_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406183.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVS"
BASE COUNT          201 a           91 c          121 g          168 t
ORIGIN      
        1 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
       61 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      121 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      181 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      241 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      301 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      361 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      421 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      481 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      541 cttccgttgg gaagtggaat gcagtatgag agctcggttt c
//
LOCUS       DACERL010000252          567 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.268, whole genome shotgun sequence.
ACCESSION   DACERL010000252 DACERL010000000
VERSION     DACERL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.268"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>567
                     /locus_tag="IXF54_002999"
     CDS             <1..>567
                     /locus_tag="IXF54_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1406184.1"
                     /translation="MFTMFDEQAEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQY
                     GETMTGGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEK
                     GKDFIPFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAAS
                     YQTHLDKVVYQVQLVSPQLKGTMKAEFKT"
BASE COUNT          182 a          104 c          135 g          146 t
ORIGIN      
        1 gatgtttacc atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact
       61 accatcgtta aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg
      121 agggacacaa tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa
      181 aaatagtgta cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc
      241 gaccgttctt aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt
      301 tgaaaaggga aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt
      361 agacggatct gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga
      421 agcatccagt ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc
      481 ctatcaaaca cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa
      541 aggaacgatg aaagcagaat ttaagac
//
LOCUS       DACERL010000253          558 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.269, whole genome shotgun sequence.
ACCESSION   DACERL010000253 DACERL010000000
VERSION     DACERL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.269"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>558
                     /locus_tag="IXF54_003000"
     CDS             <1..>558
                     /locus_tag="IXF54_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1406185.1"
                     /translation="EDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEI
                     TFTDQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPK
                     NIVSKLSFELTKFGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTAT
                     DLTLGKYYFVEVPSEVVVGSDQEPTA"
BASE COUNT          202 a           87 c          127 g          142 t
ORIGIN      
        1 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
       61 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
      121 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
      181 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
      241 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
      301 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
      361 agaccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
      421 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
      481 tgatttgacg ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
      541 agatcaagaa ccaacagc
//
LOCUS       DACERL010000254          558 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.270, whole genome shotgun sequence.
ACCESSION   DACERL010000254 DACERL010000000
VERSION     DACERL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.270"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>558)
                     /locus_tag="IXF54_003001"
     CDS             complement(<1..>558)
                     /locus_tag="IXF54_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1406186.1"
                     /translation="EDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEI
                     TFADQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPK
                     NIVSKLSFELTKFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTAT
                     DLTLGKYYFVEVPSEVVVGSDKEPTA"
BASE COUNT          146 a          123 c           87 g          202 t
ORIGIN      
        1 gctgttggtt ctttatctga tcccactaca acttctgaag gaacttccac aaaatagtat
       61 tttcctaacg tcaaatcagt agcagtcagc tttccttgat catccgatac aagatctcct
      121 aattttgttc cttttccagg ttcgcccttg tataactcaa attttgcccc ttttaaaggt
      181 gtttgtttgc tttgagctgt gccatcgtca ccaaatttcg ttaattcaaa actcaactta
      241 gaaacaatat ttttaggata taagtgaatg tcttttaaaa aacctgaacc tgtattatca
      301 gtcattggcg caatcacaac cattggtttt gctttttgtg tgactaatcc agcagcggat
      361 ttactttcat aaaccacata tacattccct ttggaattga cataggctgg ttgatcagca
      421 aaagtgatct caccattttc atctacagct gaagtaattt tgttggttgc attcttgacg
      481 tactcactgt tagatccctt gtcttctaca tctttaacga tttcgtccac cttcgcgttg
      541 gttagatcag aatcttca
//
LOCUS       DACERL010000255          552 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.271, whole genome shotgun sequence.
ACCESSION   DACERL010000255 DACERL010000000
VERSION     DACERL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.271"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>552
                     /locus_tag="IXF54_003002"
     CDS             <1..>552
                     /locus_tag="IXF54_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1406187.1"
                     /translation="VSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRPVFQEVLD
                     YVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGNPLLDKFI
                     KDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQRRLVYKN
                     IVEDLKKGVAIAKIAKDYNVTRQT"
BASE COUNT          194 a          105 c          112 g          141 t
ORIGIN      
        1 tgtctcatcg atcgatcaaa atgaacaacg ccaagttgaa gaaatgaaaa aattcggtgc
       61 agagagaatt ttcatcgaga aacaatctgg cgctacaatt actcatcgac ctgttttcca
      121 agaagtattg gattacgtcc gagatcaaga tatttttatt gtggaagcta ttgatcgact
      181 tggaagaaat tacgacgaga tcatcgcttc agtcaattac ttgaagaaga aaaatgtgca
      241 gctgatcatt acaagtctac cgatcatggc cgaagcaatt ggcaatcctc tcctggataa
      301 attcattaaa gatttgatca ttcaaatttt ggccatgatc gctgaacaag aacgtaccga
      361 atctaaacga cgtcaggcac aaggcattaa gattgcgaaa gcgaacggtg tttataaagg
      421 acgtcctaag ctatacagtg ccaatgcaaa agatccccaa cgtcgtcttg tttataaaaa
      481 catcgttgag gatctaaaaa aaggtgttgc gatcgctaag attgcaaaag actacaatgt
      541 tacgagacaa ac
//
LOCUS       DACERL010000256          549 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.272, whole genome shotgun sequence.
ACCESSION   DACERL010000256 DACERL010000000
VERSION     DACERL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.272"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            1..549
                     /locus_tag="IXF54_003003"
                     /pseudo
     CDS             1..549
                     /locus_tag="IXF54_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          202 a          112 c          115 g          120 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      241 tctcgtcaaa cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      301 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      361 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      421 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac agcttgtaat
      481 gaagcgaaaa cagcgaatgt attagatcgg acctttacac aagaacagcc attggaagcc
      541 atcgttacg
//
LOCUS       DACERL010000257          544 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.273, whole genome shotgun sequence.
ACCESSION   DACERL010000257 DACERL010000000
VERSION     DACERL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.273"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(251..544)
                     /locus_tag="IXF54_003004"
                     /pseudo
     CDS             complement(251..544)
                     /locus_tag="IXF54_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          172 a           91 c           96 g          185 t
ORIGIN      
        1 cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt agagaaagat
       61 acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt ttgttttact
      121 tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa atttcgatag
      181 ccgtaggcta cttgtattgt gcaatagttt tttacaaaaa aaaggggtgc ggatattttt
      241 ttggaccttt ttaacaaata tatctttgct tcttccgata ttgaagaggg cttagccctc
      301 ctaaagataa cttaattcgt tgattattat accaatgaat ataattatcc aatttgttta
      361 taaactgatt gatcgttgta ttcttccagt cacgttgata aaacatctcg ttttttaatc
      421 gtccaaagaa accttcgcac gctgaattat ctggtgaata acccttttta gacatagagt
      481 gaagcaagtt gtctttgttc caatagtcca actaacaata gcaccatcgt aacaatcgac
      541 aagt
//
LOCUS       DACERL010000258          539 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.274, whole genome shotgun sequence.
ACCESSION   DACERL010000258 DACERL010000000
VERSION     DACERL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.274"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>539)
                     /locus_tag="IXF54_003005"
     CDS             complement(<1..>539)
                     /locus_tag="IXF54_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HAQ1406188.1"
                     /translation="RLFLWLFGTSAGLITILITVFVTLFLILAAGSIDNSDSDSSSGG
                     EAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTS
                     ASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSN
                     FNACVGNKSPGASLLILGRG"
BASE COUNT          173 a          131 c           94 g          141 t
ORIGIN      
        1 cctcgtccta agatcaataa gcttgcgcca gggcttttat ttcctacaca agcattaaaa
       61 tttgattgtg tggctaccat aaagccgtaa taggaaaatg cagcttttga gttgggaaac
      121 ttcatgtacc agccaccttc aatgccacca attcctcgtg cagttccttt aggaaacaga
      181 caaccatcaa accaagtaat cccaaaatag ttgagatcat ttcttgcgga cgccgatgta
      241 ccaaacgctg attcgtaacc gtattgcgaa aggatcacac ttggtaataa tttatatttc
      301 actgctgcac ctacggcata ttgcgcaatc tcatcagaaa aagggcctct tcctttgatt
      361 tctttataaa tcggtaagcc ttccgaatat tctcctgtaa aagcttcgcc accactgctt
      421 gaatctgaat ccgaattatc aatcgagccc gccgcaagta ttagaaatag ggtcacgaac
      481 acagtgatca aaatagtaat caaacctgca gaagtaccaa acaaccacaa aaataagcg
//
LOCUS       DACERL010000259          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.275, whole genome shotgun sequence.
ACCESSION   DACERL010000259 DACERL010000000
VERSION     DACERL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.275"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>528
                     /locus_tag="IXF54_003006"
     CDS             <1..>528
                     /locus_tag="IXF54_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ1406189.1"
                     /translation="DMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNE
                     LRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGF
                     STSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA
                     MIYPYTNGKIEAKNT"
BASE COUNT          179 a          113 c          101 g          135 t
ORIGIN      
        1 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
       61 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      121 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      181 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      241 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      301 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
      361 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
      421 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaagaaggca tcaccaacgc
      481 gatgatctat ccttacacca atggaaaaat agaagcgaag aataccca
//
LOCUS       DACERL010000260          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.276, whole genome shotgun sequence.
ACCESSION   DACERL010000260 DACERL010000000
VERSION     DACERL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.276"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(5..90)
                     /locus_tag="IXF54_003007"
     tRNA            complement(5..90)
                     /locus_tag="IXF54_003007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXF54_003008"
     tRNA            complement(107..178)
                     /locus_tag="IXF54_003008"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXF54_003009"
     tRNA            complement(190..262)
                     /locus_tag="IXF54_003009"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXF54_003010"
     tRNA            complement(280..361)
                     /locus_tag="IXF54_003010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXF54_003011"
     tRNA            complement(369..441)
                     /locus_tag="IXF54_003011"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXF54_003012"
     tRNA            complement(445..517)
                     /locus_tag="IXF54_003012"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACERL010000261          527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.277, whole genome shotgun sequence.
ACCESSION   DACERL010000261 DACERL010000000
VERSION     DACERL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.277"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>527
                     /locus_tag="IXF54_003013"
     CDS             <1..>527
                     /locus_tag="IXF54_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAQ1406190.1"
                     /translation="IIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPED
                     KLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTEL
                     NKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTV
                     TDNRSDADKYEPTVEG"
BASE COUNT          210 a           82 c          122 g          113 t
ORIGIN      
        1 atcattgaaa acccagggga taacattccg gctggctatc ataaagtaac ctttactgca
       61 ggagaaggaa caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc
      121 ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg
      181 ccagaggaag caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca
      241 aaactggatg ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca
      301 gaattgaata aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa
      361 gatactaagt atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg
      421 actgttgtag tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg
      481 acagacaatc gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DACERL010000262          523 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.278, whole genome shotgun sequence.
ACCESSION   DACERL010000262 DACERL010000000
VERSION     DACERL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.278"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>523
                     /locus_tag="IXF54_003014"
     rRNA            <1..>523
                     /locus_tag="IXF54_003014"
                     /product="23S ribosomal RNA"
BASE COUNT          158 a          115 c          153 g           97 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gac
//
LOCUS       DACERL010000263          522 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.279, whole genome shotgun sequence.
ACCESSION   DACERL010000263 DACERL010000000
VERSION     DACERL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.279"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            152..412
                     /locus_tag="IXF54_003015"
     CDS             152..412
                     /locus_tag="IXF54_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406191.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          179 a           61 c           71 g          211 t
ORIGIN      
        1 aagtcctctt ttctgtgaat tggttgtcgt taactttatt ctacagaagc gacttccttt
       61 tttgtatgga ttttttcatt tacacaaaat attttacact ctcaattatt ttaatataag
      121 tacttcagat ttaataaagg agaaatatct aatgaaaaaa attgatttaa taaatatgat
      181 aggtatgtta atagggatat tagtaaatat agtaattttc acagattggc tcggggtgtt
      241 attttctaat cttattccca tacttattat aggcatttgt ggaattatac tttcaatatt
      301 agaacttttt gaaagcagaa atacaatgaa tagaatattt gcttgtatta tattaattgt
      361 taatctattg cctatggtct attttacttt tttatatttt gcactgggat gaaataaatt
      421 aaccaaaagt ttctttttct tataatgtgt atcaatatta aaactgaaaa agatatggat
      481 aataggatca aagccctcat tggaacactc aatatattat ca
//
LOCUS       DACERL010000264          520 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.280, whole genome shotgun sequence.
ACCESSION   DACERL010000264 DACERL010000000
VERSION     DACERL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.280"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          176 a           72 c           88 g          184 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggataacg tgattttttg ctgtaatgac
       61 aaggttttag ctgtttcaga taccatgtat ccaaaaatag tttattgaga aaataggaga
      121 gtatttgata ctaactagta ggaaaaaatg agataaaaag taaatggaat catttaaggt
      181 aatctgaaat aaaaaatata cagccctgtg gtactttacc tacaaggctg tatatatttt
      241 ttactctcag cgtttttgac tccgtgtctg actccgtttt attgattttg aatgttattc
      301 aatgaccatt taacaggtca aatttgatgt ttttgattcc gtatgacgcc gtttgatatc
      361 tagtgatata gtgatttgag tctacatcga ataagggaat aaaccaataa aaaatgattt
      421 agaatattaa aataacaaaa aagaggacta cctgtattag tcctcttttt tgaccagatt
      481 ttgaccagtc tattttacat cctagtatat ttatatgagc
//
LOCUS       DACERL010000265          514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.281, whole genome shotgun sequence.
ACCESSION   DACERL010000265 DACERL010000000
VERSION     DACERL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.281"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            132..344
                     /locus_tag="IXF54_003016"
     CDS             132..344
                     /locus_tag="IXF54_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406192.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          171 a           82 c           96 g          165 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaa
//
LOCUS       DACERL010000266          513 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.282, whole genome shotgun sequence.
ACCESSION   DACERL010000266 DACERL010000000
VERSION     DACERL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.282"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            17..>513
                     /locus_tag="IXF54_003017"
     CDS             17..>513
                     /locus_tag="IXF54_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1406193.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          187 a           93 c           85 g          148 t
ORIGIN      
        1 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
       61 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      121 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      181 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      241 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      301 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      361 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      421 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      481 taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DACERL010000267          511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.283, whole genome shotgun sequence.
ACCESSION   DACERL010000267 DACERL010000000
VERSION     DACERL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.283"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..132)
                     /locus_tag="IXF54_003018"
     CDS             complement(<1..132)
                     /locus_tag="IXF54_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1406194.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          184 a           93 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag a
//
LOCUS       DACERL010000268          510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.284, whole genome shotgun sequence.
ACCESSION   DACERL010000268 DACERL010000000
VERSION     DACERL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.284"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..465
                     /locus_tag="IXF54_003019"
     CDS             <1..465
                     /locus_tag="IXF54_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406195.1"
                     /translation="TQNNDQLVSGNFTLKGEGQLTFYLLPTIAEMKTAIRRKDKIQPT
                     VTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSERDSPSRILKRIKFPPYLEQI
                     VVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLHANKRPKKIAATKK"
BASE COUNT          165 a          111 c          102 g          132 t
ORIGIN      
        1 actcaaaaca atgatcaact ggtatctggc aacttcacac taaaaggtga aggacagttg
       61 actttctatc tattgccgac gatagctgaa atgaaaacag caattcgccg caaagacaaa
      121 atccaaccaa ccgtgacaat gagcagaaaa acatctgata agattcaagg tgtggctttt
      181 tatggccatt atctcttagc cagtcgttct tatggtcctt atacctcgga actcttagtg
      241 tccgagcggg actctcctag ccggatccta aaacgaatca aatttccacc gtatttggaa
      301 caaatcgttg ttgtagaaga tcgtctagca gtattgtttg aaagtggcgc cgcagcgtat
      361 cgcgagaaag ccaatcctgt gttagccaat gttttattac tagacttagc aacgttgctt
      421 catgcaaata agcgtcccaa aaagattgct gcaactaaaa aatagtcgga caactcaaga
      481 tgatcactgt tgttcataaa gaacgacaag
//
LOCUS       DACERL010000269          510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.285, whole genome shotgun sequence.
ACCESSION   DACERL010000269 DACERL010000000
VERSION     DACERL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.285"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..508
                     /locus_tag="IXF54_003020"
     CDS             <1..508
                     /locus_tag="IXF54_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ1406196.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DACERL010000270          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.286, whole genome shotgun sequence.
ACCESSION   DACERL010000270 DACERL010000000
VERSION     DACERL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.286"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>487)
                     /locus_tag="IXF54_003021"
     CDS             complement(<1..>487)
                     /locus_tag="IXF54_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1406197.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACERL010000271          485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.287, whole genome shotgun sequence.
ACCESSION   DACERL010000271 DACERL010000000
VERSION     DACERL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.287"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..411)
                     /locus_tag="IXF54_003022"
     CDS             complement(<1..411)
                     /locus_tag="IXF54_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406198.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          182 a           78 c           62 g          163 t
ORIGIN      
        1 aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt tgaaattttt
       61 atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc ctggagttgt
      121 caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact tataattata
      181 tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt
      241 gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct
      301 atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc
      361 tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtcaa
      421 tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta ttttctcaaa
      481 aacat
//
LOCUS       DACERL010000272          484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.288, whole genome shotgun sequence.
ACCESSION   DACERL010000272 DACERL010000000
VERSION     DACERL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.288"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            44..250
                     /locus_tag="IXF54_003023"
     CDS             44..250
                     /locus_tag="IXF54_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406199.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          212 a           62 c           50 g          160 t
ORIGIN      
        1 aggtaaaaaa ataaaaaaat gtgacaagtg ttttgaatca gctgtggtaa agtttcctca
       61 atttaaacta aatccagctg cctcgataca atttcaagga catgtatttg atgttttagc
      121 taaagataac ataaaaaaat acaaaaaagg taatatattc catattgata tacaaatatc
      181 ttctaattta gaagatataa aaaaagcctt taaaattaaa acaattggca ctgctcctaa
      241 ttttaaataa caaaaaaatt actagataat ctaaaaacag gatggcatta tgagaacaat
      301 aactaaactt cctatatcac atattttgta attagtgaaa agcaaattga ctactatcat
      361 aagttaatga ctctcatctt aatatttaaa atatattcat atttttctat tttatctaaa
      421 tgcgattttc atatctaata atagaaaaat aaacaattaa caataaaaaa gtcttcattt
      481 attg
//
LOCUS       DACERL010000273          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.289, whole genome shotgun sequence.
ACCESSION   DACERL010000273 DACERL010000000
VERSION     DACERL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.289"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..428)
                     /locus_tag="IXF54_003024"
     CDS             complement(<1..428)
                     /locus_tag="IXF54_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1406200.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           91 c           59 g          178 t
ORIGIN      
        1 gctcttaaac gaataacttc ttcttcaaga gactcatctt taagtttttt aggaatgttt
       61 agcttggaat tcatactaat ttttctgccc cttttttggc tctcaagtga agaagcaccg
      121 ccttcttcat attgctctat ccatttactt agagttctat ttgaaccgat attaaatttt
      181 ttagcagttt cttggataga aagaccattt gtttccatat attctataac atcaagttta
      241 aattttgtag tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa
      301 atccttaccc aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg
      361 tagccgcctt cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat
      421 ttccgcaaaa aaagcacccc ttttaattag atttctagtc
//
LOCUS       DACERL010000274          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.290, whole genome shotgun sequence.
ACCESSION   DACERL010000274 DACERL010000000
VERSION     DACERL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.290"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(20..91)
                     /locus_tag="IXF54_003025"
     tRNA            complement(20..91)
                     /locus_tag="IXF54_003025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXF54_003026"
     tRNA            complement(113..200)
                     /locus_tag="IXF54_003026"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXF54_003027"
     tRNA            complement(225..298)
                     /locus_tag="IXF54_003027"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXF54_003028"
     tRNA            complement(312..382)
                     /locus_tag="IXF54_003028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXF54_003029"
     tRNA            complement(384..459)
                     /locus_tag="IXF54_003029"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACERL010000275          454 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.291, whole genome shotgun sequence.
ACCESSION   DACERL010000275 DACERL010000000
VERSION     DACERL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.291"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(116..400)
                     /locus_tag="IXF54_003030"
     CDS             complement(116..400)
                     /locus_tag="IXF54_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1406201.1"
                     /translation="MNQMKNEKDQPFDPDYLNRCMKTVVKENKEIREITIHGFRHTHC
                     SLLFEAGATLKEVQDRLGHANSDITLKIYTHVTTEAKRETANKLALYLDS"
BASE COUNT          123 a           68 c           74 g          189 t
ORIGIN      
        1 attgtgtttt atgtgtctta cgtttgttta atatgtatgg aaagccttta ataggtgaat
       61 agaaaattta tttttgttga actaaaaaag aagctaattc gtatgaacta gcttcttagc
      121 tatctaaata taaagcaagt ttatttgctg tttctctttt agcttcagtt gtgacgtgag
      181 tataaatctt caaagttata tcactattag catgacctag acggtcttgg acttctttca
      241 atgttgctcc tgcctcgaat aacaatgaac aatgagtgtg tctaaaaccg tggattgtga
      301 tttctcgtat ttctttattt tctttaacaa cagttttcat acaacgattc aaatagtcgg
      361 ggtcaaatgg ttggtctttt tcattcttca tttgattcac ctcttttcct tttttatttt
      421 tacgaggatg attataccat ttttggttat ctaa
//
LOCUS       DACERL010000276          448 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.292, whole genome shotgun sequence.
ACCESSION   DACERL010000276 DACERL010000000
VERSION     DACERL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.292"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..347
                     /locus_tag="IXF54_003031"
     CDS             <1..347
                     /locus_tag="IXF54_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAQ1406202.1"
                     /translation="DFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLN
                     MVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTW
                     IDPLPFLNSSKK"
BASE COUNT          140 a           62 c          114 g          132 t
ORIGIN      
        1 tagattttgg ttcagttgat catccaggaa gtgagattca tgcggttcat ggcggaaaag
       61 tcgtgtacgt tggcaatcct ggtatttcag gtttgggcgc gtgtgtgatc gtgattaatt
      121 acgatggctt gaatatggtg tatcaagaat ttgcaaatag tacaggaaat tctcgagtga
      181 aagtaggcga tcaagtgaaa gttggtcaag taattgggat acgagataca gcacacttgc
      241 atttaggatt tactcgaatg gattggcgtc aagcacaagg acatgcattc attgatgatg
      301 gtacatggat cgatccgtta ccatttttga atagcagtaa aaaatagatc aggagaaaaa
      361 atatggaaaa taaaacgtta aaaatcgtct taacagctgc aattagcgtt agtttaatcg
      421 gcaatattgc gttgttttgg cagctgaa
//
LOCUS       DACERL010000277          434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.293, whole genome shotgun sequence.
ACCESSION   DACERL010000277 DACERL010000000
VERSION     DACERL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.293"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..395
                     /locus_tag="IXF54_003032"
     CDS             <1..395
                     /locus_tag="IXF54_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406203.1"
                     /translation="QPVEIIESKLKDRAKKLSYDQLKKVAEGETKTIDDQEGVIEFSI
                     EVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLYPKSEKSEPKEE
                     IKKVVSTGVKKNFFENCWEFFAQLFFDN"
BASE COUNT          175 a           51 c           81 g          127 t
ORIGIN      
        1 atcaacctgt agaaataatt gagtcaaaat tgaaagatcg agcaaaaaaa ctttcatacg
       61 accagttgaa aaaagtggct gaaggagaaa ccaaaacgat tgatgatcag gaaggcgtga
      121 ttgaattttc cattgaagta cctgcaaaca aaaaacaagc atactatatc gttaatgaat
      181 caagccctga aaacatttca aacagtgaag atattgtttt attgacacct gtttcaaatg
      241 aaaaaggtga atttttgacg gatgtttggt tataccctaa atcagagaaa tcagagccga
      301 aagaagaaat taaaaaagtt gtgtcaactg gtgtaaagaa gaactttttt gaaaattgtt
      361 gggaattttt tgctcaatta ttttttgata attaggatga aatgatgaaa ataataagaa
      421 tattagtttt taat
//
LOCUS       DACERL010000278          432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.294, whole genome shotgun sequence.
ACCESSION   DACERL010000278 DACERL010000000
VERSION     DACERL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.294"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            142..>432
                     /locus_tag="IXF54_003033"
     CDS             142..>432
                     /locus_tag="IXF54_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406204.1"
                     /translation="MLKELANLNYRVEVKTAKGDYQAIRAKVIKVSDDYFAVKLTHVP
                     EEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVHEDKVKDQVKDEDYEQHAINYK"
BASE COUNT          170 a           68 c           92 g          102 t
ORIGIN      
        1 acgaatcaga aggtagatat accagatgag gtaagcacga acgccttttt aaacacgaaa
       61 gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc aattagtttt tgaatttgcg
      121 atcgacgatg aagatcaaag cttgttaaaa gaactggcta atcttaatta tcgcgtagaa
      181 gtaaagacag ctaaagggga ttatcaagca atacgagcca aagtgatcaa ggtgtctgat
      241 gattattttg cagtgaagct aacgcatgta ccagaagaat atatcgccat gcgtttaacc
      301 attattcctg aaaaaattga tccaaaagtg gatatgcaag aaccacaaga tctgatttat
      361 tacgtgcacg aagataaggt taaagatcaa gtgaaagatg aagattatga acaacacgcg
      421 atcaattaca aa
//
LOCUS       DACERL010000279          430 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.295, whole genome shotgun sequence.
ACCESSION   DACERL010000279 DACERL010000000
VERSION     DACERL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.295"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..312
                     /locus_tag="IXF54_003034"
     CDS             <1..312
                     /locus_tag="IXF54_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1406205.1"
                     /translation="ARTGGTMGKSFLVKEISEESVFASYLIRIRLVEKLLSEYVDCFL
                     DSPLYWKLLEKISYGTGQPNVNGTNLSKLLIPLPPLEEQQRMTTKIEMIRRSIRRINF
                     E"
BASE COUNT          156 a           56 c           75 g          143 t
ORIGIN      
        1 gcaaggacag gaggaactat gggaaaaagt ttcttggtta aggaaataag tgaggaatct
       61 gtttttgctt cttacttgat tcgaattagg ttagttgaga aattattatc agaatacgta
      121 gactgcttct tagactcgcc attatattgg aagttattag aaaaaatttc ttacggaact
      181 gggcaaccaa atgtaaatgg tacaaattta tctaaactac ttataccatt accaccttta
      241 gaagaacaac aacgcatgac tactaaaata gaaatgataa gaagatctat tcgtagaatc
      301 aattttgagt aaatataacg gacctagcat ccattttata ccagatgatg ctaggtctat
      361 tatttatatt ttatggaatt taattgattt gttctagtgt tttaatatta ttacgacagt
      421 aaaaaaatag
//
LOCUS       DACERL010000280          421 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.296, whole genome shotgun sequence.
ACCESSION   DACERL010000280 DACERL010000000
VERSION     DACERL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.296"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..390
                     /locus_tag="IXF54_003035"
     CDS             <1..390
                     /locus_tag="IXF54_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1406206.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DACERL010000281          395 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.297, whole genome shotgun sequence.
ACCESSION   DACERL010000281 DACERL010000000
VERSION     DACERL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.297"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            1..395
                     /locus_tag="IXF54_003036"
                     /pseudo
     CDS             1..395
                     /locus_tag="IXF54_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           83 c           67 g          122 t
ORIGIN      
        1 ccatacttaa tgcgacgaca atggatttat tcttagcaca atcatcactc ctagaaatat
       61 tcatgacagt caagtcgcct ttcagctcat caaacaatcg aagagacttt ttcctgaaat
      121 caattgtgtt gtcgctgatg ctgagtataa gacacctaag tttgttcact ttttgactca
      181 tttaaacctt cgaccttgtt tgccttattc aaggcccaaa gggaaaaagg ggctattatc
      241 aaaaaatgag tttctctatg acgagtattt tgattgttat atttgtccgc aagatcaaat
      301 gctcgctttt tcaacggtta ctcgagaaag gtaccgagaa tacaaatcca atccaaaaga
      361 atgtgtgaac tgtcctctgc taaaccaatg tacca
//
LOCUS       DACERL010000282          395 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.298, whole genome shotgun sequence.
ACCESSION   DACERL010000282 DACERL010000000
VERSION     DACERL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.298"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>395
                     /locus_tag="IXF54_003037"
     CDS             <1..>395
                     /locus_tag="IXF54_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406207.1"
                     /translation="HTSCDDNGFILSTIITPGNIYDSQVAFQLVKQSKRLFPEINCVV
                     ADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLA
                     FSTVTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           83 c           72 g          123 t
ORIGIN      
        1 ccatacttca tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat
       61 ttatgacagt caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat
      121 caattgtgtt gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca
      181 tttgaacctt cgaccttgtt tgccttattc aaggcccaaa gggaaaaagg ggctattatc
      241 aaaaaatgag tttctctatg acgagtattt tgattgttat atttgtccgc aagatcaaat
      301 gctcgctttt tcaacggtta ctcgagaagg gtaccgagaa tacaaatcca atccaaaaga
      361 atgtgtgaac tgtcctctgc taaaccaatg tacca
//
LOCUS       DACERL010000283          390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.299, whole genome shotgun sequence.
ACCESSION   DACERL010000283 DACERL010000000
VERSION     DACERL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.299"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            93..323
                     /locus_tag="IXF54_003038"
     CDS             93..323
                     /locus_tag="IXF54_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406208.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
BASE COUNT          169 a           46 c           75 g          100 t
ORIGIN      
        1 tgaaagatca agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
       61 aaaagattta gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag
      121 atactttcgc tgagcaaaaa gtattgatta aagcctatct caaagttgta aaagctaaag
      181 aaatatgtcg agaagtgaat ttatctaaca caacaatatc tgagttgaag aatggtcgaa
      241 agaatttaga tactttaaaa ttcagatcgt ttgaagctct atatgtagct gctaaaaaat
      301 attttacggc tgaaaggaaa taaaaacagg aggattttat gtgaaaaaag tatatcaaaa
      361 atgtttatgg atcagctata tcaccgtaac
//
LOCUS       DACERL010000284          386 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.300, whole genome shotgun sequence.
ACCESSION   DACERL010000284 DACERL010000000
VERSION     DACERL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.300"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            93..323
                     /locus_tag="IXF54_003039"
     CDS             93..323
                     /locus_tag="IXF54_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406209.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT          171 a           45 c           69 g          101 t
ORIGIN      
        1 tgaaagatca agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
       61 aaaagattta gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag
      121 atactttcgc tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag
      181 aaatatgtcg agaagtgaat ttatctaata caacaatatc tgagttgaag aatggtcgaa
      241 agaatttaga tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat
      301 attttacagc tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa
      361 aatgcttatg aatcagctat atcacc
//
LOCUS       DACERL010000285          381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.301, whole genome shotgun sequence.
ACCESSION   DACERL010000285 DACERL010000000
VERSION     DACERL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.301"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(21..94)
                     /locus_tag="IXF54_003040"
     tRNA            complement(21..94)
                     /locus_tag="IXF54_003040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IXF54_003041"
     tRNA            complement(108..181)
                     /locus_tag="IXF54_003041"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IXF54_003042"
     tRNA            complement(209..281)
                     /locus_tag="IXF54_003042"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IXF54_003043"
     tRNA            complement(301..374)
                     /locus_tag="IXF54_003043"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACERL010000286          368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.302, whole genome shotgun sequence.
ACCESSION   DACERL010000286 DACERL010000000
VERSION     DACERL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.302"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          126 a           41 c           62 g          139 t
ORIGIN      
        1 gcgaaccaag agtatagtta atatcagttt cggtgttatt ttccattaaa agaattgaag
       61 ttatggagtg ttcaaaatca gttttcgcaa aattaattgc ttgttcttca attggttggg
      121 agttgatatt gaaattagaa ttttctgaag cataggtatt tgtaattcct atactaaaaa
      181 ttccaatcat caaggaaaag aggagaccta gataaatttt ctttttgggt ttcaaaataa
      241 tcacgccttt gtgttaaatt taagtggtat acacctataa ttaaaatata caatatgcga
      301 taacaaattt ttgttatagt tcataaaata ttgttttggg aacataatcg ttatttatag
      361 cgttagtt
//
LOCUS       DACERL010000287          367 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.303, whole genome shotgun sequence.
ACCESSION   DACERL010000287 DACERL010000000
VERSION     DACERL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.303"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>367
                     /locus_tag="IXF54_003044"
     CDS             <1..>367
                     /locus_tag="IXF54_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406210.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DACERL010000288          361 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.304, whole genome shotgun sequence.
ACCESSION   DACERL010000288 DACERL010000000
VERSION     DACERL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.304"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>361
                     /locus_tag="IXF54_003045"
     CDS             <1..>361
                     /locus_tag="IXF54_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1406211.1"
                     /translation="KANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDT
                     VASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGA
                     KFLKVDSGLFGTGIKATP"
BASE COUNT          145 a           58 c           67 g           91 t
ORIGIN      
        1 aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga aaatacagat
       61 tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa taatggccaa
      121 gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta taacatgtat
      181 gtaaatgaca gcgcagctgt tgccaatcca ttaacaaata gcgtggattt cgtttataac
      241 aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt tgtgacttat
      301 ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa agcaacaccg
      361 c
//
LOCUS       DACERL010000289          360 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.305, whole genome shotgun sequence.
ACCESSION   DACERL010000289 DACERL010000000
VERSION     DACERL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.305"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>360)
                     /locus_tag="IXF54_003046"
     CDS             complement(<1..>360)
                     /locus_tag="IXF54_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1406212.1"
                     /translation="VEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVN
                     SVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVT
                     YEQVLSIFNSYLLFNGR"
BASE COUNT          107 a           61 c           70 g          122 t
ORIGIN      
        1 ttcctcccgt taaataatag ataactatta aaaatagaca atacttgctc ataagtaacg
       61 gtacttaaat tgtttacttt ggcgtgtttc attgcttgat gaaactgatt tttagtaaac
      121 agttgacgat attctcgatt gacccatttt gaaacaaagt acgtatatag cttccaatat
      181 ttatctggaa catctgtggt atggcgggta agttttatta agacactgtt tacttttggt
      241 ttaggatgaa agcattccgc tggcagctta agcaattgct gaatcgagac ttgagtgtgc
      301 aagagcaacc ctagtgttcg gtgaatatcc aaggtacgct tgtagaatcc ttcttcaaca
//
LOCUS       DACERL010000290          342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.306, whole genome shotgun sequence.
ACCESSION   DACERL010000290 DACERL010000000
VERSION     DACERL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.306"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..311)
                     /locus_tag="IXF54_003047"
     CDS             complement(<1..311)
                     /locus_tag="IXF54_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406213.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VT"
BASE COUNT          102 a           73 c           34 g          133 t
ORIGIN      
        1 gtaacctcct cataatcagt tagtactata aaggtatctt tgtttttatc caaaaaatct
       61 aataaatctt ttgtagaaga tatctcttca tcaacttcac cataataaat accttcgata
      121 attgttgcta tatctgaaaa actaattttt actggacatt tatcgtctac cgcattcaat
      181 accgacaaaa ttgcttcttt ttctgcaatc tctcgtttga atttctttgg agctaatcgc
      241 atgacactca agtcattttc aagcttttca atccatgcag ttttactcat tttcgcaaat
      301 ttttgaatca ttcctctacc acctttgcta tctactaaat ta
//
LOCUS       DACERL010000291          340 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.307, whole genome shotgun sequence.
ACCESSION   DACERL010000291 DACERL010000000
VERSION     DACERL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.307"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>340
                     /locus_tag="IXF54_003048"
     CDS             <1..>340
                     /locus_tag="IXF54_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406214.1"
                     /translation="GKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLV
                     ERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHD
                     IDIYFADPGTP"
BASE COUNT          107 a           68 c           59 g          106 t
ORIGIN      
        1 ggggaaaaca acagtatcag cgctcaatcc atgatcgacc tgataattat cctgatttca
       61 attctgagtt tggtcacctt gaaggtgata cgatcgttgg cattcatcat aaaagtgccg
      121 tcattacttt agttgaaaga ttatctaaag tcattatcac gattaaaccc aacggccgta
      181 aggcattaga tattgaaact gcccttaatc aatggttttc tcgcttccct aaaaacttct
      241 ttaaatctat tacgtttgac tgtggaaaag aattttctaa ctggaaagcc attagtaacc
      301 aacatgatat tgatatatat tttgcggacc ctggaacacc
//
LOCUS       DACERL010000292          339 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.308, whole genome shotgun sequence.
ACCESSION   DACERL010000292 DACERL010000000
VERSION     DACERL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.308"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..202
                     /gene="tnpB"
                     /locus_tag="IXF54_003049"
     CDS             <1..202
                     /gene="tnpB"
                     /locus_tag="IXF54_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1406215.1"
                     /translation="QDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVR
                     WLLEGLSIDQPRAILPGKKGVF"
BASE COUNT          102 a           57 c           78 g          102 t
ORIGIN      
        1 tcaagaccgt ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg
       61 gatagaaaac ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa
      121 ccaacaggtt cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc
      181 aggaaaaaaa ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat
      241 ttcttgtgcg tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa
      301 attttggaaa ctacagatac gttgcttcag ttgcttcaa
//
LOCUS       DACERL010000293          337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.309, whole genome shotgun sequence.
ACCESSION   DACERL010000293 DACERL010000000
VERSION     DACERL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.309"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>337
                     /locus_tag="IXF54_003050"
     CDS             <1..>337
                     /locus_tag="IXF54_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1406216.1"
                     /translation="FVCAIGDEEMTIKERVSFPTTTPEETMPLVIDFFKQYQADLAGI
                     GIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAYGE
                     YVFGSGKGL"
BASE COUNT           96 a           70 c           81 g           90 t
ORIGIN      
        1 aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga
       61 caacgacacc tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag
      121 atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca
      181 cttatgggta cattacctcg accccgaaat tggcatggca aaattttgat ttcatcggta
      241 ccatgaaaaa agaatttccg atcccgattt cttggacaac agatgtcaat gcggctgcct
      301 atggtgagta tgtcttcggc agtggcaaag gattgag
//
LOCUS       DACERL010000294          333 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.310, whole genome shotgun sequence.
ACCESSION   DACERL010000294 DACERL010000000
VERSION     DACERL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.310"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>333
                     /locus_tag="IXF54_003051"
     CDS             <1..>333
                     /locus_tag="IXF54_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1406217.1"
                     /translation="AGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASY
                     IAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPA
                     LEDNAGTLG"
BASE COUNT           98 a           72 c           82 g           81 t
ORIGIN      
        1 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
       61 gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc
      121 gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg
      181 atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca
      241 tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc
      301 ccagcattag aagacaacgc tggaacgctc ggt
//
LOCUS       DACERL010000295          332 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.311, whole genome shotgun sequence.
ACCESSION   DACERL010000295 DACERL010000000
VERSION     DACERL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.311"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>332
                     /locus_tag="IXF54_003052"
     CDS             <1..>332
                     /locus_tag="IXF54_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1406218.1"
                     /translation="VMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTF
                     LSIYSLNGQYQQRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKA
                     PALSTNNLT"
BASE COUNT          125 a           49 c           65 g           93 t
ORIGIN      
        1 gtcatgaatg caaaactaga atttaagtat gcaggaacaa aaacaaaagt agctttgagt
       61 gaaccacagc cagaaattca atcaaaaatg agatcgatga gagcagctgg tattgattta
      121 aacgatacat ttctatccat ttatagttta aatggacagt atcagcaacg tgtgtcttgg
      181 tataatgaca ataatgaatc tgtcggtgaa cgtaatattg atatgagaga atttgttggg
      241 tatgaaaaaa tgggtagctt accttatttt gtcacaacag atacagcatg tgcagaatac
      301 aaagctcctg cgttatcaac aaacaattta ac
//
LOCUS       DACERL010000296          326 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.312, whole genome shotgun sequence.
ACCESSION   DACERL010000296 DACERL010000000
VERSION     DACERL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.312"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..293
                     /locus_tag="IXF54_003053"
     CDS             <1..293
                     /locus_tag="IXF54_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1406219.1"
                     /translation="VTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIK
                     RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          108 a           61 c           70 g           87 t
ORIGIN      
        1 ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag agtgtgggtt
       61 tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata gaacaagacc
      121 atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc tcttccacga
      181 ttaagggcat ggagaccatt cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct
      241 tcggcttttc ggtgtctact gaaatcaagg tattaatggg aataacagcc taagatattt
      301 ggagttcaga gagggcgcgt ttgatt
//
LOCUS       DACERL010000297          324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.313, whole genome shotgun sequence.
ACCESSION   DACERL010000297 DACERL010000000
VERSION     DACERL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.313"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            28..237
                     /locus_tag="IXF54_003054"
     CDS             28..237
                     /locus_tag="IXF54_003054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406220.1"
                     /translation="MDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEKERLRKEFDYW
                     RTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          127 a           45 c           74 g           78 t
ORIGIN      
        1 gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag gtatgtacag
       61 ctgttagctc gtggttattc acctaaagat gtattcaaat ccctgtctaa aggaacagat
      121 atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa tggaatcaag
      181 gatttgaaac cagcacggcc agtaattaaa cgaaagaaaa agataaccca agactgaagt
      241 gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacaaggaa ggtaggaaaa
      301 ctcatgaatt atgaagaaat cgaa
//
LOCUS       DACERL010000298          323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.314, whole genome shotgun sequence.
ACCESSION   DACERL010000298 DACERL010000000
VERSION     DACERL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.314"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..180
                     /locus_tag="IXF54_003055"
     CDS             <1..180
                     /locus_tag="IXF54_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1406221.1"
                     /translation="KIEASDEYHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTT
                     TTTTKPTTTTTTTQA"
BASE COUNT          118 a           60 c           56 g           89 t
ORIGIN      
        1 aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa
       61 caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct
      121 ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag
      181 gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt
      241 gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc
      301 agacacgatg atcagattcc tat
//
LOCUS       DACERL010000299          322 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.315, whole genome shotgun sequence.
ACCESSION   DACERL010000299 DACERL010000000
VERSION     DACERL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.315"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(54..302)
                     /locus_tag="IXF54_003056"
     CDS             complement(54..302)
                     /locus_tag="IXF54_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1406222.1"
                     /translation="MHWLWVLIVGAIIGLIAGAITNKGGSMGWIANIVAGLVGSAIGQ
                     ALLGSWGPQLAGMALIPSIIGAIIVVAVVSFFLGRSRG"
BASE COUNT          107 a           79 c           48 g           88 t
ORIGIN      
        1 agcgaaaagc tgctccttaa gataataggt aaaattgctg atatatttga cgattaacct
       61 cttgaacgtc ccaagaaaaa tgatactaca gcaacaacga tgatcgcacc aatgatagaa
      121 ggaatcaaag ccattcctgc taattgagga ccccagctac ctaacaatgc ttgtccaatt
      181 gctgaaccaa ctaaacctgc aacgatgtta gcgatccatc ccattgatcc acctttattt
      241 gtaattgctc cggcaatcaa accgataata gctcctacga ttaataccca taaccaatgc
      301 ataattttct ctttctttct gt
//
LOCUS       DACERL010000300          322 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.316, whole genome shotgun sequence.
ACCESSION   DACERL010000300 DACERL010000000
VERSION     DACERL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.316"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..267
                     /locus_tag="IXF54_003057"
     CDS             <1..267
                     /locus_tag="IXF54_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAQ1406223.1"
                     /translation="DYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSLENETAKKL
                     LIQFYEEQAADRLQEYVEKLIKNKEISEMIGKRLLLESEKVVKE"
BASE COUNT          121 a           39 c           69 g           93 t
ORIGIN      
        1 gattatttcc taccagatga atatgctaga aatctctatc atggactgca tgatcaatta
       61 gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga gaatgagacg
      121 gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact tcaagaatat
      181 gttgaaaagt tgatcaaaaa taaagaaatt tctgaaatga ttgggaaacg gttgttattg
      241 gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc atagccgact
      301 ggtggtcagg cttattcgga ga
//
LOCUS       DACERL010000301          322 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.317, whole genome shotgun sequence.
ACCESSION   DACERL010000301 DACERL010000000
VERSION     DACERL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.317"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            95..>322
                     /locus_tag="IXF54_003058"
     CDS             95..>322
                     /locus_tag="IXF54_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1406224.1"
                     /translation="MFGLLFEKMLQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKT
                     SFGKVNIDVPRDREASFEPELIPNRKRDVSDI"
BASE COUNT          103 a           58 c           70 g           91 t
ORIGIN      
        1 taaaaacgcc aaacattccc gattctattc tataggtcgg gttatttgac gtacaggtac
       61 tgttctatgg ggcgcttacg atgttttaaa agatgtgttt ggtctcttat ttgaaaaaat
      121 gcttcaagga gaattgaata accatttagg gtatgacgcc cattctaaag agccaaagaa
      181 acatgacaat cgacgaaacg gttatggcaa taagacgctt aaaacgagct ttggtaaagt
      241 aaacattgat gttcccagag accgggaggc ttcttttgaa cctgagttga ttcctaatag
      301 aaaacgagat gtctccgaca tc
//
LOCUS       DACERL010000302          321 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.318, whole genome shotgun sequence.
ACCESSION   DACERL010000302 DACERL010000000
VERSION     DACERL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.318"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            149..>321
                     /locus_tag="IXF54_003059"
     CDS             149..>321
                     /locus_tag="IXF54_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1406225.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          140 a           47 c           47 g           87 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt t
//
LOCUS       DACERL010000303          314 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.319, whole genome shotgun sequence.
ACCESSION   DACERL010000303 DACERL010000000
VERSION     DACERL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.319"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(15..194)
                     /locus_tag="IXF54_003060"
     CDS             complement(15..194)
                     /locus_tag="IXF54_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406226.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT           96 a           71 c           44 g          103 t
ORIGIN      
        1 aatgaaaagt gacgtcactt tttgagtgct ggtacatcaa atcctgcttt ttcttgcgcg
       61 atcaatagat ctaacgcaat acacataatc ttagattcac tttttccgta cttctgcttt
      121 agtttctgca attctttttg tctcttttca tccaacgtaa cgggtaaccg tttatttcct
      181 attgcaattg gcattagtac tctctccttt catcgaaata accctatgaa aaatatggct
      241 gcaatcaact tgaatattag actcacaagg gctaacacaa agcgccaaaa gatcaaacaa
      301 aacaccagta ttac
//
LOCUS       DACERL010000304          311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.320, whole genome shotgun sequence.
ACCESSION   DACERL010000304 DACERL010000000
VERSION     DACERL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.320"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>311
                     /locus_tag="IXF54_003061"
     CDS             <1..>311
                     /locus_tag="IXF54_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406227.1"
                     /translation="SSLIDEGLNISKSFYGDVTAANKADKTGSPAFQIIQNNVIDVST
                     LLATDPNKVESLPTDKRNALTAKNFKTANINGIITPEQAKGMAGNDKSGLSDIQKDRL
                     KA"
BASE COUNT          123 a           49 c           61 g           78 t
ORIGIN      
        1 agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca
       61 gcaaataaag cagataaaac gggatcacct gcttttcaaa ttattcaaaa taatgtgatc
      121 gatgtcagta cgttactagc aactgacccg aataaagtag aatccctacc aacagataaa
      181 agaaatgctt taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct
      241 gaacaagcta aaggtatggc aggtaatgat aaaagtgggt tatcagatat acaaaaggat
      301 cggttaaagg c
//
LOCUS       DACERL010000305          311 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.321, whole genome shotgun sequence.
ACCESSION   DACERL010000305 DACERL010000000
VERSION     DACERL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.321"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>311
                     /locus_tag="IXF54_003062"
     CDS             <1..>311
                     /locus_tag="IXF54_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406228.1"
                     /translation="SSLIDEGLNISKSFYGDVTAANKADKTGSPAFQIIQNNVIDVST
                     LLATDPNKVESLPTDKRNALTAKNFKTANINGIITPKQAKEMADKDKSGLSDLQKDRL
                     RA"
BASE COUNT          124 a           50 c           59 g           78 t
ORIGIN      
        1 agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca
       61 gcaaataaag cagataaaac gggttcacct gcttttcaaa ttattcaaaa taatgtgatc
      121 gatgtcagta cattactagc aactgacccg aataaagtag aatccctgcc aacagacaaa
      181 agaaatgctt taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct
      241 aaacaagcta aagaaatggc ggataaagat aagagtggtt tatcagattt acaaaaggat
      301 cggttaagag c
//
LOCUS       DACERL010000306          310 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.322, whole genome shotgun sequence.
ACCESSION   DACERL010000306 DACERL010000000
VERSION     DACERL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.322"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>310
                     /locus_tag="IXF54_003063"
     CDS             <1..>310
                     /locus_tag="IXF54_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ1406229.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     "
BASE COUNT          134 a           44 c           52 g           80 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca
//
LOCUS       DACERL010000307          305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.323, whole genome shotgun sequence.
ACCESSION   DACERL010000307 DACERL010000000
VERSION     DACERL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.323"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>305
                     /locus_tag="IXF54_003064"
     CDS             <1..>305
                     /locus_tag="IXF54_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1406230.1"
                     /translation="DAAFKGLQHKPETPKEYTVTINHIDNQTGQVIATDQAKVTSGST
                     YTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQINLLCNNNYK"
BASE COUNT          128 a           55 c           46 g           76 t
ORIGIN      
        1 gatgctgcat ttaaagggtt gcaacacaaa cctgaaacac caaaagaata tacggttact
       61 attaaccata ttgacaatca aacaggtcaa gtgattgcta cagatcaagc aaaagtaact
      121 tctggttcta cttacactgc taaggcaaaa gcatttaaat atagcgaagg cgctaaagat
      181 aattttagct acaaagtgat tggtaatgat actcaaagca aaacaatcac aggtaataca
      241 acaatcacat tcaattataa tcaagtgcat caaatcaatt tgttgtgcaa taacaactac
      301 aaaaa
//
LOCUS       DACERL010000308          305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.324, whole genome shotgun sequence.
ACCESSION   DACERL010000308 DACERL010000000
VERSION     DACERL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.324"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>305
                     /locus_tag="IXF54_003065"
     CDS             <1..>305
                     /locus_tag="IXF54_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1406231.1"
                     /translation="DAAFKGLQHKPETPKEYMVTINHIDNQTGQVIATDQAKVTSGST
                     YTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQINLLCNNNYK"
BASE COUNT          125 a           54 c           49 g           77 t
ORIGIN      
        1 gatgctgcat ttaaagggtt gcaacacaaa cctgaaacgc caaaagaata tatggttacc
       61 attaaccata ttgacaatca aacaggtcaa gtgattgcta cagatcaagc aaaagtgact
      121 tctggttcta cttacactgc taaggcaaaa gcatttaaat atagcgaagg tgctaaagat
      181 aattttagct acaaagtgat tggtaatgat actcaaagca aaacaatcac aggtaataca
      241 acgatcacat tcaattataa tcaagtgcat caaatcaatt tgttgtgcaa taacaactac
      301 aaaaa
//
LOCUS       DACERL010000309          301 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.325, whole genome shotgun sequence.
ACCESSION   DACERL010000309 DACERL010000000
VERSION     DACERL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.325"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..267
                     /locus_tag="IXF54_003066"
     CDS             <1..267
                     /locus_tag="IXF54_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAQ1406232.1"
                     /translation="DYFLPDIYARRLYHNMHDQIEEEAKENIFIKIVQSLDNSTAQKL
                     LIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKAVKE"
BASE COUNT          114 a           46 c           57 g           84 t
ORIGIN      
        1 gattattttc tacctgatat ctatgcacgt cgactttatc acaatatgca cgatcaaata
       61 gaagaggaag ctaaagaaaa catttttatt aaaatcgttc aatctttaga caactcaacg
      121 gcacaaaagt tacttattca attttatgaa gagaagcaaa tagatcgttt gcaagaatac
      181 cttgaaaaat tgcgtcaaaa tgaagaaatt acagaggtta taaggaaacg acttttgcta
      241 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat attttccggc attgccgact
      301 g
//
LOCUS       DACERL010000310          290 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.326, whole genome shotgun sequence.
ACCESSION   DACERL010000310 DACERL010000000
VERSION     DACERL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.326"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            19..>290
                     /locus_tag="IXF54_003067"
     CDS             19..>290
                     /locus_tag="IXF54_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406233.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DACERL010000311          289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.327, whole genome shotgun sequence.
ACCESSION   DACERL010000311 DACERL010000000
VERSION     DACERL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.327"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          125 a           49 c           50 g           65 t
ORIGIN      
        1 acgaagaatt gtttgtaaaa caaacggata aataataaaa aagactgctc tttcgagcag
       61 ccagttaaaa aataatgagt gtttattctt gaaaaagcaa cgcgcttatg aagcgctacc
      121 aaaaatttat catattttat gaaaaataac accccagtaa aaaatgactg gtaaaaattc
      181 acaaagaaac ggaaagaagc aactgcagaa aatcaagcct taaaaagagc ttttgaccta
      241 gaccaatgaa aaatcacaaa gtgagcccct gtatcccttg gtagagagg
//
LOCUS       DACERL010000312          289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.328, whole genome shotgun sequence.
ACCESSION   DACERL010000312 DACERL010000000
VERSION     DACERL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.328"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(67..267)
                     /locus_tag="IXF54_003068"
     CDS             complement(67..267)
                     /locus_tag="IXF54_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406234.1"
                     /translation="MAKAKTPFEKAASKILERRGVSETQWKQEVLEKAQFDLLRGKDK
                     DLEGYIYQREREKLVFDEIIKH"
BASE COUNT           70 a           59 c           47 g          113 t
ORIGIN      
        1 ctactacttt cggttgtgtt aaaaaattca ttgtaattga ttcaaacata aaattgttcg
       61 ctccttttag tgtttaataa tttcatcgaa aacaagcttt tcacgttcac gttgatagat
      121 atatccttct aagtccttgt cttttccccg taataggtca aattgtgctt tttcaaggac
      181 ttcttgtttc cactgtgttt cagatacacc gcggcgttct aagattttag acgcggcttt
      241 ttcaaatggc gttttagctt ttgccactgt gattcctcct tcaggacaa
//
LOCUS       DACERL010000313          280 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.329, whole genome shotgun sequence.
ACCESSION   DACERL010000313 DACERL010000000
VERSION     DACERL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.329"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            166..>280
                     /locus_tag="IXF54_003069"
     CDS             166..>280
                     /locus_tag="IXF54_003069"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406235.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLE"
BASE COUNT           95 a           48 c           56 g           81 t
ORIGIN      
        1 ttgttcctgt ccaattagga aacgaaaaga gtgcaccaga aaaaggtaaa cccgcggctg
       61 ccaagttagc gatgcgcatc aaaagagaga atggttctga agttttgatc tataacggta
      121 ttaacaatta tattcttcaa gctattttga aagaactgtt gtccaatgct ttatgattta
      181 cttcaaacca aacatattta tattgtgtgc ggaaaaacag atttgcgaaa aggaattgat
      241 ggtcttgcca gtcttattca acaagagtat caactcgagc
//
LOCUS       DACERL010000314          279 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.330, whole genome shotgun sequence.
ACCESSION   DACERL010000314 DACERL010000000
VERSION     DACERL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.330"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>279
                     /locus_tag="IXF54_003070"
     CDS             <1..>279
                     /locus_tag="IXF54_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1406236.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENA"
BASE COUNT           94 a           53 c           60 g           72 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaa
//
LOCUS       DACERL010000315          275 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.331, whole genome shotgun sequence.
ACCESSION   DACERL010000315 DACERL010000000
VERSION     DACERL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.331"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            13..>275
                     /locus_tag="IXF54_003071"
     CDS             13..>275
                     /locus_tag="IXF54_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406237.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQG"
BASE COUNT           95 a           44 c           70 g           66 t
ORIGIN      
        1 ggaggcaaaa aaatgacaca gcgggtagca ataatgattt tagtgttgct ggtcattggc
       61 ctccttgttt actatgtcct aaagtttaag cattggaagc agcaacggat acatcaggat
      121 attgaaaaaa agttaaaacg gtatcctatt gttcaagcag cttgggaaaa ggctgaagca
      181 aaacaatata acattcctgg attaacagaa acaagaatgg tggtgccgga aaccggcgaa
      241 aatgaggttt gtcaatggat gacgccgcaa gggtt
//
LOCUS       DACERL010000316          273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.332, whole genome shotgun sequence.
ACCESSION   DACERL010000316 DACERL010000000
VERSION     DACERL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.332"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          106 a           44 c           45 g           78 t
ORIGIN      
        1 cggcgaaaaa aataggcata ttaagcgggc taggaggaga aatattcaca tatcattttt
       61 tcattccgca agctgcgtca agttatttgg aatttgtaga acagatgaga gaggtagaag
      121 cagcactaca aactactttt caataagtag gaattataga aattttactc aaaaaatctt
      181 cattaaaaaa tctgactaaa taagttgaga aaacagaata tacttcaata tccagtgatt
      241 tataaatatc attcactcat tacccagcgc ctt
//
LOCUS       DACERL010000317          270 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.333, whole genome shotgun sequence.
ACCESSION   DACERL010000317 DACERL010000000
VERSION     DACERL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.333"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            89..>270
                     /locus_tag="IXF54_003072"
     CDS             89..>270
                     /locus_tag="IXF54_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1406238.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           56 c           43 g           87 t
ORIGIN      
        1 cttttaccta tgaaatattt tcacgtgtta aagtattcct cgttgaacac aacaaataaa
       61 actttttaac gaataaagga gatttttcat gacatcactt aaaactttac tttttggaac
      121 aactttggct gccggcgctg cattcttcat gggaacgact gctcatgcgg acgaagctta
      181 taccgttcaa tcaggtgata ctctatcaac gatttctcaa aaatacgttg gtgataactc
      241 cttgatcaat gccattgcag aatcaaattc
//
LOCUS       DACERL010000318          267 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.334, whole genome shotgun sequence.
ACCESSION   DACERL010000318 DACERL010000000
VERSION     DACERL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.334"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>267
                     /locus_tag="IXF54_003073"
     CDS             <1..>267
                     /locus_tag="IXF54_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406239.1"
                     /translation="SRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVL"
BASE COUNT          111 a           49 c           46 g           61 t
ORIGIN      
        1 tatcacgtca gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag
       61 aagtagtcgc tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa
      121 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
      181 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      241 atgaagccaa aacgacaaac gtattga
//
LOCUS       DACERL010000319          266 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.335, whole genome shotgun sequence.
ACCESSION   DACERL010000319 DACERL010000000
VERSION     DACERL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.335"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(34..>266)
                     /locus_tag="IXF54_003074"
     CDS             complement(34..>266)
                     /locus_tag="IXF54_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406240.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DACERL010000320          263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.336, whole genome shotgun sequence.
ACCESSION   DACERL010000320 DACERL010000000
VERSION     DACERL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.336"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(69..263)
                     /locus_tag="IXF54_003075"
                     /pseudo
     CDS             complement(69..263)
                     /locus_tag="IXF54_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           65 a           62 c           40 g           96 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atc
//
LOCUS       DACERL010000321          259 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.337, whole genome shotgun sequence.
ACCESSION   DACERL010000321 DACERL010000000
VERSION     DACERL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.337"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>259)
                     /locus_tag="IXF54_003076"
     rRNA            complement(<1..>259)
                     /locus_tag="IXF54_003076"
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           70 c           72 g           64 t
ORIGIN      
        1 cgggtgttac aaactctcgt ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc
       61 accgcggcgt gctgatccgc gattactagc gattccggct tcatgcaggc gagttgcagc
      121 ctgcaatccg aactgagaga agctttaaga gattagctta gcctcgcgac ttcgcaactc
      181 gttgtacttc ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac
      241 gtcatcccca ccttcctcc
//
LOCUS       DACERL010000322          259 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.338, whole genome shotgun sequence.
ACCESSION   DACERL010000322 DACERL010000000
VERSION     DACERL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.338"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>259)
                     /locus_tag="IXF54_003077"
     CDS             complement(<1..>259)
                     /locus_tag="IXF54_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1406241.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTR"
BASE COUNT           75 a           48 c           52 g           84 t
ORIGIN      
        1 cgtgttaaac gctgtaaggc catgtatttc tcttctttga taggggaagt tgtgaatcgt
       61 tgaatacgca agaaatcagc gattcttaat gcatcgtttc gatcagtttt attctcatca
      121 aacatgcgac taaattgttt cacccgaaaa ggattttcaa tcgttacaag tgtgttgagc
      181 ttctttagtt tctcatcttc atgaaaaaac atggaaggat ggaagctgta catggacgtt
      241 gactccatgc caatcacaa
//
LOCUS       DACERL010000323          256 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.339, whole genome shotgun sequence.
ACCESSION   DACERL010000323 DACERL010000000
VERSION     DACERL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.339"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          110 a           24 c           50 g           72 t
ORIGIN      
        1 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
       61 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
      121 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
      181 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
      241 taacacatag agctga
//
LOCUS       DACERL010000324          253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.340, whole genome shotgun sequence.
ACCESSION   DACERL010000324 DACERL010000000
VERSION     DACERL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.340"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>253
                     /locus_tag="IXF54_003078"
     CDS             <1..>253
                     /locus_tag="IXF54_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406242.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTP"
BASE COUNT           84 a           51 c           45 g           73 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc ctt
//
LOCUS       DACERL010000325          252 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.341, whole genome shotgun sequence.
ACCESSION   DACERL010000325 DACERL010000000
VERSION     DACERL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.341"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>252
                     /locus_tag="IXF54_003079"
     CDS             <1..>252
                     /locus_tag="IXF54_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406243.1"
                     /translation="NRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSIT
                     NPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT           94 a           47 c           41 g           70 t
ORIGIN      
        1 aaatagacaa gcaaaactat tgaatcaaat agccaaagtt ggctataact caacaaaaaa
       61 atcttatttt tatggtctta aaattcatat gatcgttact aagactggct ttccgattac
      121 ttactcaatc acgaatccag gtgtccatga cgtgaaagtg ttagagactt tatcggaaga
      181 agcgaatctt cccaatatcc taggggacaa aggatatatt agccataaaa tccatgaaaa
      241 gcttgccctt aa
//
LOCUS       DACERL010000326          252 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.342, whole genome shotgun sequence.
ACCESSION   DACERL010000326 DACERL010000000
VERSION     DACERL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.342"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           96 a           39 c           28 g           89 t
ORIGIN      
        1 ttttacctac tttctgcaac tatttaaaac acaccctaat catacatgag aaaaccagat
       61 ttttcaatgt ttgtcatttt tctagataaa tggcataaat tttcagcgct aagtgacaaa
      121 agcaactaat ttttcatgat agatttttaa atatctcaat ctatctccaa aattagttgc
      181 ttttgtagct atagttgact atgaatatga caaataatta gaaaaaactg tgccaattaa
      241 atataaaaat ga
//
LOCUS       DACERL010000327          248 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.343, whole genome shotgun sequence.
ACCESSION   DACERL010000327 DACERL010000000
VERSION     DACERL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.343"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>248
                     /locus_tag="IXF54_003080"
     CDS             <1..>248
                     /locus_tag="IXF54_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1406244.1"
                     /translation="NDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDT
                     ALLWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDV"
BASE COUNT           86 a           49 c           50 g           63 t
ORIGIN      
        1 aatgatcatt tcaccgatgt tgtaggagct gatacttatc acagaagtgg tgtaacgtat
       61 acgcttcaag gcgcttcccc aacattcatg attggcgcaa atacgaatag tatgatgttt
      121 agctttgata ctgcattgct atggacacca caaccatcga agcctacaaa agaagtgttt
      181 aacaaagcta atactgaaga ggcagcacac aatattgaca aaaaagtgat tccacaagga
      241 tcagatgt
//
LOCUS       DACERL010000328          243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.344, whole genome shotgun sequence.
ACCESSION   DACERL010000328 DACERL010000000
VERSION     DACERL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.344"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT          105 a           31 c           45 g           62 t
ORIGIN      
        1 aagactgttt caagtgaatg gttagattat tttgaaaata tcaataagta tggcgcatat
       61 tacaccatga aaaaggaaga caacgaaaat gaataaggtg atttatcaac taacaacgga
      121 ttccatatca acgatttgaa agaaaaaaag gtcaataatc atttataccg aaaagtgaga
      181 atgtgtgaaa taaggacaaa aaaagcctag taactaaggg aatggttctt atttcgcacc
      241 aat
//
LOCUS       DACERL010000329          237 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.345, whole genome shotgun sequence.
ACCESSION   DACERL010000329 DACERL010000000
VERSION     DACERL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.345"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..216)
                     /locus_tag="IXF54_003081"
     CDS             complement(<1..216)
                     /locus_tag="IXF54_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1406245.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DACERL010000330          234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.346, whole genome shotgun sequence.
ACCESSION   DACERL010000330 DACERL010000000
VERSION     DACERL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.346"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            95..>234
                     /locus_tag="IXF54_003082"
     CDS             95..>234
                     /locus_tag="IXF54_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1406246.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQA"
BASE COUNT           89 a           30 c           53 g           62 t
ORIGIN      
        1 gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt
       61 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
      121 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
      181 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggc
//
LOCUS       DACERL010000331          233 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.347, whole genome shotgun sequence.
ACCESSION   DACERL010000331 DACERL010000000
VERSION     DACERL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.347"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DACERL010000332          232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.348, whole genome shotgun sequence.
ACCESSION   DACERL010000332 DACERL010000000
VERSION     DACERL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.348"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>232)
                     /locus_tag="IXF54_003083"
     CDS             complement(<1..>232)
                     /locus_tag="IXF54_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406247.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DACERL010000333          230 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.349, whole genome shotgun sequence.
ACCESSION   DACERL010000333 DACERL010000000
VERSION     DACERL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.349"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>230
                     /locus_tag="IXF54_003084"
     CDS             <1..>230
                     /locus_tag="IXF54_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406248.1"
                     /translation="DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWL
                     TITGSATGRVAALRLIDILADTSEETGQPIAY"
BASE COUNT           70 a           47 c           52 g           61 t
ORIGIN      
        1 gaccatcagc atcattctat tattcatgta ctggatagag aaacaggaca gccgatcaaa
       61 ctgctgattt tacctaaacg gccacatgta gggggcttag tatatgatac aaaacgggaa
      121 cttttatggt tgacaatcac aggatctgct actggacgag tagccgcttt acgtttgatc
      181 gatatcttag cagatacatc tgaagagacg ggacagccaa tcgcttattg
//
LOCUS       DACERL010000334          228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.350, whole genome shotgun sequence.
ACCESSION   DACERL010000334 DACERL010000000
VERSION     DACERL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.350"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(3..>228)
                     /locus_tag="IXF54_003085"
     CDS             complement(3..>228)
                     /locus_tag="IXF54_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1406249.1"
                     /translation="IIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKL
                     NEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT           75 a           38 c           23 g           92 t
ORIGIN      
        1 aactatttaa tggactgttt ccgatattct actggagata gtccatttaa ctttagtttt
       61 atccttttgt tattatacca cctaatatac tcatttaatt tactttgaaa tatctcaatt
      121 gatcggaatt tttctcgata aaaaaactct gatttaagca caccaaaaaa attttctatt
      181 acagaattat ctaagcaatt tccttttcta gacatgcttt gaataatg
//
LOCUS       DACERL010000335          227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.352, whole genome shotgun sequence.
ACCESSION   DACERL010000335 DACERL010000000
VERSION     DACERL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.352"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>227
                     /locus_tag="IXF54_003086"
     CDS             <1..>227
                     /locus_tag="IXF54_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406250.1"
                     /translation="NGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNER
                     GLKGTAEDLTEKAKDTLEEKLDGTRAKELAE"
BASE COUNT           92 a           36 c           56 g           43 t
ORIGIN      
        1 ataacgggga agaacaatca gacgataaag ctacaactgt acctaatagt aagaaaacag
       61 tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt
      121 acgtcaatga acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca
      181 ccttagaaga gaaattagac ggcacgagag ctaaagaatt ggctgaa
//
LOCUS       DACERL010000336          227 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.353, whole genome shotgun sequence.
ACCESSION   DACERL010000336 DACERL010000000
VERSION     DACERL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.353"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>227)
                     /locus_tag="IXF54_003087"
     rRNA            complement(<1..>227)
                     /locus_tag="IXF54_003087"
                     /product="23S ribosomal RNA"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DACERL010000337          224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.354, whole genome shotgun sequence.
ACCESSION   DACERL010000337 DACERL010000000
VERSION     DACERL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.354"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>224
                     /locus_tag="IXF54_003088"
     CDS             <1..>224
                     /locus_tag="IXF54_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1406251.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DACERL010000338          220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.356, whole genome shotgun sequence.
ACCESSION   DACERL010000338 DACERL010000000
VERSION     DACERL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.356"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..175)
                     /locus_tag="IXF54_003089"
     rRNA            complement(<1..175)
                     /locus_tag="IXF54_003089"
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DACERL010000339          218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.357, whole genome shotgun sequence.
ACCESSION   DACERL010000339 DACERL010000000
VERSION     DACERL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.357"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..>218
                     /locus_tag="IXF54_003090"
     CDS             <1..>218
                     /locus_tag="IXF54_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406252.1"
                     /translation="VFSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVN
                     ALFQSLISKVLALFCVVTLIYLGLKFFM"
BASE COUNT           65 a           25 c           41 g           87 t
ORIGIN      
        1 gtatttagtt ggatttggaa cacttttctg tggggattga ttaaaggatt atataatctc
       61 aatactttac tagaaaaaac catttatcaa agctttgatt taaaagactt attgaacgcc
      121 gctggggtaa atgcactatt tcaaagtttg attagtaagg tattggctct tttttgtgtc
      181 gtcacattaa tttatttagg cttgaaattt tttatgag
//
LOCUS       DACERL010000340          216 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.358, whole genome shotgun sequence.
ACCESSION   DACERL010000340 DACERL010000000
VERSION     DACERL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.358"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>216)
                     /locus_tag="IXF54_003091"
     CDS             complement(<1..>216)
                     /locus_tag="IXF54_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406253.1"
                     /translation="TISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQLHV
                     LGQVLEQENQSSVKLTLTLHDKEGKIE"
BASE COUNT           53 a           42 c           29 g           92 t
ORIGIN      
        1 ttttctattt ttccttcttt atcatgtaac gtgagagtca atttaacaga agattgattc
       61 tcttgttcta acacttgtcc taacacatgt agctgttctt ccgtttcaat gacagaatat
      121 ttaattgttt tcggtaaaaa ggaagtacgc tttcccactt tcttcgttgc ttttcttatt
      181 aactctttat ccaattttgc attgcctgaa atcgta
//
LOCUS       DACERL010000341          206 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.359, whole genome shotgun sequence.
ACCESSION   DACERL010000341 DACERL010000000
VERSION     DACERL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.359"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(<1..>206)
                     /locus_tag="IXF54_003092"
     CDS             complement(<1..>206)
                     /locus_tag="IXF54_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1406254.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DACERL010000342          203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.360, whole genome shotgun sequence.
ACCESSION   DACERL010000342 DACERL010000000
VERSION     DACERL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.360"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           74 a           40 c           32 g           57 t
ORIGIN      
        1 aaaaatagag aattgatttt cgttcatgac aaacaaaaaa gagggaacgc atccaaacat
       61 gcgttccctc taatttcagc gttcgtcata cggcaaagag ccacacggac atttttataa
      121 cattatgctt aggcatagat tcatcgtaat atcacgtatt tttaacctta acacagccaa
      181 aaaaagtggt atcacttttg aat
//
LOCUS       DACERL010000343          200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.361, whole genome shotgun sequence.
ACCESSION   DACERL010000343 DACERL010000000
VERSION     DACERL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.361"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           66 a           35 c           35 g           64 t
ORIGIN      
        1 agatccaagt ccgcatgtgt acaatgtatg tctatatcat tgatatccca cgatggattg
       61 aaatcttcaa tatattgata agcataagga agattcgttt ccccatgatt aaaaatagga
      121 tacggccgta ttttatgacc tttcaaatct tgtcgtttta aaggagcgag tacaatcgtt
      181 tcaccttcta aaaaagtaga
//
LOCUS       DACERL010000344          200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.362, whole genome shotgun sequence.
ACCESSION   DACERL010000344 DACERL010000000
VERSION     DACERL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.362"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           72 a           32 c           48 g           48 t
ORIGIN      
        1 atattgtgtg caaacatttt ggaatggaca cgtcggagaa agctattcct tatattgcag
       61 catggacgaa gaatggtcag acgttggatg ataaggaagc tgcggaacgt gggaaaatca
      121 tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac catttctaat gaaatcaatc
      181 aatacagaga aagagaacca
//
LOCUS       DACERL010000345          200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.denovo.363, whole genome shotgun sequence.
ACCESSION   DACERL010000345 DACERL010000000
VERSION     DACERL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.denovo.363"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
BASE COUNT           87 a           26 c           40 g           47 t
ORIGIN      
        1 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
       61 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
      121 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
      181 cgtttcaaaa aacgaaaaag
//
LOCUS       DACERL010000346        41492 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.020, whole genome shotgun sequence.
ACCESSION   DACERL010000346 DACERL010000000
VERSION     DACERL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41492
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.020"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(1223..1888)
                     /locus_tag="IXF54_003095"
     CDS             complement(1223..1888)
                     /locus_tag="IXF54_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1406255.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2014..4050
                     /locus_tag="IXF54_003096"
     CDS             2014..4050
                     /locus_tag="IXF54_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1406256.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4054..4692
                     /locus_tag="IXF54_003097"
     CDS             4054..4692
                     /locus_tag="IXF54_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1406257.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4720..5148
                     /locus_tag="IXF54_003098"
     CDS             4720..5148
                     /locus_tag="IXF54_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406258.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5605..6516
                     /locus_tag="IXF54_003099"
     CDS             5605..6516
                     /locus_tag="IXF54_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1406259.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6733..7170
                     /locus_tag="IXF54_003100"
     CDS             6733..7170
                     /locus_tag="IXF54_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406260.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7265..8149
                     /locus_tag="IXF54_003101"
     CDS             7265..8149
                     /locus_tag="IXF54_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1406261.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8373..11540
                     /locus_tag="IXF54_003102"
     CDS             8373..11540
                     /locus_tag="IXF54_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406262.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11560..12105
                     /locus_tag="IXF54_003103"
     CDS             11560..12105
                     /locus_tag="IXF54_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1406263.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12133..12588
                     /locus_tag="IXF54_003104"
     CDS             12133..12588
                     /locus_tag="IXF54_003104"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1406264.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12585..13343
                     /locus_tag="IXF54_003105"
     CDS             12585..13343
                     /locus_tag="IXF54_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1406265.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13844..15043
                     /locus_tag="IXF54_003106"
     CDS             13844..15043
                     /locus_tag="IXF54_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1406266.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15065..15277
                     /locus_tag="IXF54_003107"
     CDS             15065..15277
                     /locus_tag="IXF54_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1406267.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15352..15828
                     /locus_tag="IXF54_003108"
     CDS             15352..15828
                     /locus_tag="IXF54_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ1406268.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15825..17633
                     /locus_tag="IXF54_003109"
     CDS             15825..17633
                     /locus_tag="IXF54_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1406269.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17710..17925
                     /locus_tag="IXF54_003110"
     CDS             17710..17925
                     /locus_tag="IXF54_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ1406270.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18017..18880
                     /locus_tag="IXF54_003111"
     CDS             18017..18880
                     /locus_tag="IXF54_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406271.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18911..19453
                     /locus_tag="IXF54_003112"
     CDS             18911..19453
                     /locus_tag="IXF54_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ1406272.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19467..19889
                     /locus_tag="IXF54_003113"
     CDS             19467..19889
                     /locus_tag="IXF54_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1406273.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19819..22218
                     /locus_tag="IXF54_003114"
     CDS             19819..22218
                     /locus_tag="IXF54_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ1406274.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22250..24241
                     /locus_tag="IXF54_003115"
     CDS             22250..24241
                     /locus_tag="IXF54_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ1406275.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24264..24515
                     /locus_tag="IXF54_003116"
     CDS             24264..24515
                     /locus_tag="IXF54_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ1406276.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24505..25734
                     /locus_tag="IXF54_003117"
     CDS             24505..25734
                     /locus_tag="IXF54_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ1406277.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25731..27812
                     /gene="topB"
                     /locus_tag="IXF54_003118"
     CDS             25731..27812
                     /gene="topB"
                     /locus_tag="IXF54_003118"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1406278.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            27961..31881
                     /locus_tag="IXF54_003119"
     CDS             27961..31881
                     /locus_tag="IXF54_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ1406279.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31882..32826
                     /locus_tag="IXF54_003120"
     CDS             31882..32826
                     /locus_tag="IXF54_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406280.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32830..33033
                     /locus_tag="IXF54_003121"
     CDS             32830..33033
                     /locus_tag="IXF54_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406281.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33333..33569)
                     /locus_tag="IXF54_003122"
     CDS             complement(33333..33569)
                     /locus_tag="IXF54_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1406282.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33752..34123)
                     /locus_tag="IXF54_003123"
     CDS             complement(33752..34123)
                     /locus_tag="IXF54_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406283.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34120..35448)
                     /locus_tag="IXF54_003124"
     CDS             complement(34120..35448)
                     /locus_tag="IXF54_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1406284.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35409..35738)
                     /locus_tag="IXF54_003125"
     CDS             complement(35409..35738)
                     /locus_tag="IXF54_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1406285.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(35996..36367)
                     /locus_tag="IXF54_003126"
     CDS             complement(35996..36367)
                     /locus_tag="IXF54_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1406286.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36512..36697
                     /locus_tag="IXF54_003127"
     CDS             36512..36697
                     /locus_tag="IXF54_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1406287.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37640..38299
                     /locus_tag="IXF54_003128"
     CDS             37640..38299
                     /locus_tag="IXF54_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ1406288.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38302..39645
                     /gene="vanS"
                     /locus_tag="IXF54_003129"
     CDS             38302..39645
                     /gene="vanS"
                     /locus_tag="IXF54_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1406289.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39816..40622
                     /gene="vanY"
                     /locus_tag="IXF54_003130"
     CDS             39816..40622
                     /gene="vanY"
                     /locus_tag="IXF54_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1406290.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASALEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40640..41478
                     /gene="vanW"
                     /locus_tag="IXF54_003131"
                     /pseudo
     CDS             40640..41478
                     /gene="vanW"
                     /locus_tag="IXF54_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11617 a         9537 c        10340 g         9998 t
ORIGIN      
        1 tcctcttttt tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc
       61 taattttatg attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa
      121 tttaaaaatc tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga
      181 taacatcaag gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag
      241 agccgagatt atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc
      301 ttggtgttcg tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg
      361 ttgataagga agataagata actgtgttat ataaactagg taactaactg taacaagcta
      421 attttaagag attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag
      481 gtaattgtca tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca
      541 gaatcagttg gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt
      601 cccactaaca agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat
      661 gctaccgaaa tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc
      721 cattactcaa agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata
      781 ggcgcttgaa caatccattt tgtattttat tgagtactct tggataaact accagcgatt
      841 atcatattta tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa
      901 atataaaaaa gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat
      961 attacttact agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa
     1021 tgtccatcct tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat
     1081 actataagcc agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag
     1141 gctttttcaa ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc
     1201 tatctctacg ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg
     1261 cgtcgtcata gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga
     1321 gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt
     1381 tttctacctc tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata
     1441 cccaacgttc gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg
     1501 gtttcaaaaa aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa
     1561 aaagatagac caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg
     1621 gttgttttga tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta
     1681 ttgattcggt agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta
     1741 aaagtgacag catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt
     1801 tatcggaaac accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt
     1861 aagttcgtat tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga
     1921 tttattctac atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat
     1981 tttatcaaag aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt
     2041 ttactcacaa tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa
     2101 caggtactct atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga
     2161 ttgccacaac attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat
     2221 gtctcatcag aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta
     2281 gatacatctt ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg
     2341 gatgtcaaat cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc
     2401 attgaagaat cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa
     2461 aaagaagcct atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac
     2521 actgattatg ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt
     2581 tcaaatacag acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc
     2641 cttttgttat catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat
     2701 gacttttcat tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca
     2761 ttcataattg caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt
     2821 tatttgttcc agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct
     2881 cttttattag ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt
     2941 ctttttacat ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca
     3001 actatcgttt tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct
     3061 ggtgaggact atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt
     3121 tattccaatc gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac
     3181 ggaagtatat tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga
     3241 gggataaaaa taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg
     3301 ttgatccaaa taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga
     3361 catacagatt taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa
     3421 aaagaaccga aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct
     3481 atcccctgga tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat
     3541 ttggaaagaa atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct
     3601 ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag
     3661 ttatcatttc cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt
     3721 cattacttga tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc
     3781 atgttatgca tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt
     3841 tattcgctct ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg
     3901 acaagtgtaa tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt
     3961 ttctttagct tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa
     4021 agtcagttat tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt
     4081 gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct
     4141 ggggatcttg tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata
     4201 ttaggattga atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa
     4261 caattaactg ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa
     4321 gattttgggt tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt
     4381 tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat
     4441 ataaacaaga agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc
     4501 gcctgtgcga gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg
     4561 tcattggata aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa
     4621 ggtacgttag ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag
     4681 atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt
     4741 tgtgcactag ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat
     4801 tcagttcacg gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct
     4861 aaaggtaata ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt
     4921 aatcatagct ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt
     4981 cattctaacc actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac
     5041 tctattcgat cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat
     5101 agaactgaca cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt
     5161 ttatataaaa tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt
     5221 aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct
     5281 ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa
     5341 aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg
     5401 aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct
     5461 gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt
     5521 agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa
     5581 aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta
     5641 ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa
     5701 ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat
     5761 tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta
     5821 tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt
     5881 gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa
     5941 agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa
     6001 acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa
     6061 gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc
     6121 aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc
     6181 actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa
     6241 gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt
     6301 aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa
     6361 gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa
     6421 gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca
     6481 ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac
     6541 tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt
     6601 ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc
     6661 gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt
     6721 agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta
     6781 tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata
     6841 aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc
     6901 gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca
     6961 tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac
     7021 attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata
     7081 caatgcctat cattttcttt tatttcctat atcattgttt taattttcct cagtttcata
     7141 gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac
     7201 agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa
     7261 agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga
     7321 atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca
     7381 tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca
     7441 tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga
     7501 acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg
     7561 agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt
     7621 caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg
     7681 ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact
     7741 agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt
     7801 agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa
     7861 ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca
     7921 tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt
     7981 aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt
     8041 gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt
     8101 acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg
     8161 tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt
     8221 ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca
     8281 taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa
     8341 aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg
     8401 cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga
     8461 aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag
     8521 aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag
     8581 aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag
     8641 cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac
     8701 tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt
     8761 tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca
     8821 atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt
     8881 ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta
     8941 tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat
     9001 ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg
     9061 caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac
     9121 tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg
     9181 gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg
     9241 agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc
     9301 ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg
     9361 gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca
     9421 atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca
     9481 ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc
     9541 aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg
     9601 gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga
     9661 tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag
     9721 ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa
     9781 gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc
     9841 aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg
     9901 gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag
     9961 atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag
    10021 gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta
    10081 cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg
    10141 tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc
    10201 gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga
    10261 cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt
    10321 taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca
    10381 ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct
    10441 atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg
    10501 aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta
    10561 ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag
    10621 aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg
    10681 aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac
    10741 tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca
    10801 cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt
    10861 cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg
    10921 ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg
    10981 accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa
    11041 ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac
    11101 ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt
    11161 ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg
    11221 aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat
    11281 tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga
    11341 aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt
    11401 caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg
    11461 tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca tggacattag
    11521 cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa
    11581 agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag
    11641 acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag
    11701 attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc
    11761 ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc
    11821 gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa
    11881 acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct
    11941 caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg
    12001 gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt
    12061 ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag
    12121 tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg
    12181 attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt
    12241 gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat
    12301 gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg
    12361 ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc
    12421 gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa
    12481 aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc
    12541 ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg
    12601 cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga
    12661 ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc
    12721 ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac
    12781 ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg
    12841 attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa
    12901 cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg
    12961 gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg
    13021 atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg
    13081 caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa
    13141 tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat
    13201 ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc
    13261 ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg
    13321 ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca
    13381 aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc
    13441 agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag
    13501 taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg
    13561 cgataagttg atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc
    13621 ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg
    13681 gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca
    13741 tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag
    13801 gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg
    13861 ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg
    13921 cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa
    13981 atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc
    14041 agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa
    14101 gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa
    14161 ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa
    14221 aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct
    14281 tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga
    14341 tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac
    14401 atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac
    14461 gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga
    14521 tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga
    14581 ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta
    14641 tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga
    14701 ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa
    14761 gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa
    14821 gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt
    14881 ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa
    14941 ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc
    15001 cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga
    15061 ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt
    15121 tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg
    15181 cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg
    15241 ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg
    15301 gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa
    15361 gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg
    15421 ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac
    15481 cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag
    15541 atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt
    15601 accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag
    15661 gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag
    15721 cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc
    15781 aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa
    15841 agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg
    15901 gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg
    15961 atggttttgc ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta
    16021 tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga
    16081 agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag
    16141 cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta
    16201 ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg
    16261 gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc
    16321 cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg
    16381 aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat accattaact
    16441 tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa
    16501 agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt
    16561 tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg
    16621 ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc
    16681 gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga
    16741 aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga
    16801 cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg
    16861 tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg
    16921 tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc
    16981 aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc
    17041 ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg
    17101 ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc
    17161 agtcgcagtt aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata
    17221 gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga
    17281 aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat
    17341 tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg
    17401 gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata
    17461 ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg
    17521 tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag
    17581 tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg
    17641 cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga
    17701 ggtaaattta tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga
    17761 ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac
    17821 aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg
    17881 atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc
    17941 ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg
    18001 aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat
    18061 ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga
    18121 aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct
    18181 gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat
    18241 gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc
    18301 catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg
    18361 gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt
    18421 gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt
    18481 ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct
    18541 caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat
    18601 ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg
    18661 cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat
    18721 catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat
    18781 atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa
    18841 gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca
    18901 caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta
    18961 tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga
    19021 gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc
    19081 cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa
    19141 gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct
    19201 gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa
    19261 gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa
    19321 gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct
    19381 gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct
    19441 tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc
    19501 atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg
    19561 cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg
    19621 cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc
    19681 acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa
    19741 aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg
    19801 aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag
    19861 ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc
    19921 aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc
    19981 cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt
    20041 gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg
    20101 gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac
    20161 gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac
    20221 accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac
    20281 ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc
    20341 ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc
    20401 tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt
    20461 gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc
    20521 ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc
    20581 ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa
    20641 agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc
    20701 atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc
    20761 cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc
    20821 aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt
    20881 gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc
    20941 gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga acagggcctt
    21001 gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc
    21061 agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg
    21121 tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt
    21181 tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc
    21241 gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc
    21301 gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc
    21361 aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg
    21421 gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg
    21481 ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat
    21541 ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta
    21601 gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc
    21661 tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt
    21721 gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag
    21781 atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac
    21841 gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag
    21901 cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt
    21961 tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag
    22021 gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag
    22081 tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc
    22141 tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac
    22201 gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct
    22261 gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca
    22321 ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc
    22381 ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca
    22441 taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct
    22501 gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca
    22561 gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat
    22621 ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa
    22681 gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc
    22741 aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca
    22801 tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg
    22861 aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa
    22921 ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga
    22981 taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca
    23041 gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc
    23101 acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga
    23161 aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt
    23221 gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg
    23281 cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc
    23341 cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga
    23401 ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta
    23461 tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca
    23521 gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga
    23581 aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga
    23641 cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct
    23701 gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat
    23761 gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac
    23821 cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc
    23881 cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc
    23941 tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg
    24001 gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc
    24061 gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc
    24121 ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta
    24181 cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta
    24241 acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc
    24301 agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga
    24361 aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg
    24421 aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt
    24481 accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc
    24541 cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc
    24601 ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt
    24661 ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa
    24721 aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt
    24781 tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa
    24841 aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc
    24901 ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta
    24961 gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat
    25021 tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc
    25081 ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc
    25141 agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg
    25201 aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt
    25261 attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc
    25321 gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt
    25381 accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg
    25441 accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag
    25501 gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc
    25561 ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc
    25621 gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa
    25681 accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata
    25741 aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca
    25801 cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac
    25861 atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg
    25921 aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc
    25981 agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca
    26041 ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc
    26101 aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct
    26161 ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg
    26221 ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca
    26281 agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg
    26341 ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg
    26401 cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt
    26461 gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc
    26521 ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg
    26581 ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga
    26641 gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg
    26701 gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca
    26761 tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga
    26821 agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca
    26881 agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct
    26941 gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga
    27001 ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc
    27061 gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg
    27121 agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac
    27181 gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca
    27241 cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc
    27301 agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt
    27361 ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg
    27421 atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt
    27481 cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt
    27541 gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg
    27601 cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca
    27661 ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt
    27721 ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt
    27781 acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag
    27841 tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc
    27901 ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc
    27961 gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa
    28021 ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt
    28081 ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac
    28141 agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc
    28201 cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg
    28261 caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg
    28321 gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg
    28381 aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag
    28441 tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg
    28501 agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg
    28561 gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag
    28621 gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg
    28681 aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt
    28741 tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt
    28801 ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg
    28861 tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc
    28921 ccggccccgt tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc
    28981 gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt
    29041 aaggtggtga cagtctttga catcagccag accgagggca aggattttcc catgccctat
    29101 atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg
    29161 acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc
    29221 tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc
    29281 gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag
    29341 gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat
    29401 accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat
    29461 tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg
    29521 gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag
    29581 gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg
    29641 caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg
    29701 ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac
    29761 gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg
    29821 ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt
    29881 ggagataggg acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag
    29941 gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac
    30001 aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc
    30061 cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg
    30121 gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc
    30181 aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg
    30241 caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg
    30301 accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc
    30361 taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta
    30421 caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg
    30481 gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat
    30541 tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac
    30601 accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat
    30661 cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc
    30721 aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg
    30781 gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct
    30841 gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt
    30901 tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg
    30961 gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc
    31021 ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc
    31081 gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa
    31141 aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt
    31201 gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc
    31261 aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat
    31321 gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt
    31381 gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc
    31441 tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg
    31501 acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc
    31561 aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc
    31621 gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag
    31681 ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc
    31741 gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg
    31801 gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca
    31861 aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg
    31921 gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac
    31981 attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc
    32041 cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag
    32101 tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg
    32161 gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg
    32221 cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat
    32281 gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta
    32341 ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg
    32401 ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc
    32461 accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa
    32521 gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac
    32581 tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa
    32641 tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac
    32701 gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac
    32761 agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag
    32821 gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat
    32881 gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat
    32941 atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat
    33001 gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc
    33061 catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc
    33121 ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct
    33181 tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga
    33241 aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa
    33301 agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc
    33361 aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc
    33421 tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa
    33481 tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca
    33541 tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta
    33601 tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct
    33661 acgcatttag aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc
    33721 atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt
    33781 atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc
    33841 agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca
    33901 cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg
    33961 ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc
    34021 cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc
    34081 gggccttgcg atattgaggg taattcattc gtggaatact catagctctt gcgtccattc
    34141 ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg
    34201 catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg
    34261 cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag
    34321 ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc
    34381 cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat
    34441 attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc
    34501 ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc
    34561 tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt
    34621 cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg
    34681 caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc
    34741 tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg
    34801 tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat
    34861 gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt
    34921 gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc
    34981 cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt
    35041 gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc
    35101 cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac
    35161 atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg
    35221 ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc
    35281 cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca
    35341 ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag
    35401 ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc
    35461 gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt
    35521 gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg
    35581 ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag
    35641 ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca
    35701 gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca
    35761 ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc
    35821 cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca
    35881 agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa
    35941 aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca
    36001 catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct
    36061 cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca
    36121 gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg
    36181 ctttaataag ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg
    36241 caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg
    36301 cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat
    36361 tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg
    36421 tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa
    36481 agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg
    36541 cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc
    36601 cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga
    36661 caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg
    36721 gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat
    36781 agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat
    36841 ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa
    36901 acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca
    36961 tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt
    37021 gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg
    37081 ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat
    37141 atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc
    37201 tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa
    37261 gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg
    37321 tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta
    37381 aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat
    37441 cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa
    37501 accaataata tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta
    37561 aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc
    37621 ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat
    37681 ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga
    37741 tggaaatgag gcatacacca agttttacga aaacacttat caactggtta ttcttgatat
    37801 tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac
    37861 tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc
    37921 agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga
    37981 agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact
    38041 tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga
    38101 atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat
    38161 tttgtcacgt atatggggat atgactttga gagtgatggc agcacagtcc acactcatat
    38221 caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata
    38281 ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat
    38341 acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt
    38401 gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg
    38461 gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac
    38521 aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca
    38581 ccaaatgcca atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat
    38641 gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg
    38701 acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat
    38761 atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg
    38821 gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct
    38881 catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa
    38941 atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat
    39001 gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc
    39061 ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag
    39121 ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc
    39181 atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc
    39241 ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg
    39301 tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac
    39361 acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt
    39421 tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca
    39481 ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc
    39541 atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat
    39601 ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa
    39661 atgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac
    39721 agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa
    39781 atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca
    39841 atgcggatca tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg
    39901 cgttcattat ttcgttcaca gtctgcacgc tgtttttggg gtggagactg gcttccgcat
    39961 tggaggcaac acagataccg cccatccctg caactcatac aggcagcagc actgacgtag
    40021 tggagaattt ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat
    40081 ggagcctgat tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg
    40141 agcaactatc aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt
    40201 ttgatgccgc aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag
    40261 aaaaacagca agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct
    40321 ctgcacaggc taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc
    40381 agcttggtct tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt
    40441 atagatggct ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca
    40501 agacagagat aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag
    40561 ctgccacaga gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat
    40621 gagaaaagga tgttatgtta tggacagaaa aagattgaca cagcgtttcc cgttcctgct
    40681 tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg
    40741 tcgcgctatg cgcagacgat aggggaaaaa tcgctttccc atttgctctt tgaaacggat
    40801 tgcgcattat acaaccacaa taccggattt gacatgatat accaggaaaa caaggtgttc
    40861 aacttaaagc tggcagcaaa gaccttaaac ggcttattga taagaccggg ggaaaccttt
    40921 tctttctggt ggctggtacg ccatgcggac aaagataccc cctataaaga cggccttacg
    40981 gtgaccaatg gtaaactcac caccatgtcg ggcggcggta tgtgccagat gagcaattta
    41041 ctattttgga tgttcctgca tacgccattg acaattatcc agcgcagagg tcacgaagta
    41101 aaggagtttc cagagccaaa cagtgacgag attaaagggg tggatgcaac catttcagag
    41161 ggctggattg atttaaaagt gcgaaacgat accgactgca cctaccaaat atgagtgacc
    41221 ttagatgatg agaaaatcat cggtcaggtg tccgccgaca aagagcccca agcattatac
    41281 aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg gcgggattta tgaatatgcc
    41341 caggttaaac ggatgcaagt tgccttaggt accggggaaa taatagattg caagctgctt
    41401 tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat tcagtctaat
    41461 ggtgataagt caagatgaaa tttaagattt tc
//
LOCUS       DACERL010000347        23735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.045, whole genome shotgun sequence.
ACCESSION   DACERL010000347 DACERL010000000
VERSION     DACERL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23735
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.045"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            292..567
                     /locus_tag="IXF54_003132"
     CDS             292..567
                     /locus_tag="IXF54_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406291.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2658
                     /locus_tag="IXF54_003133"
     CDS             580..2658
                     /locus_tag="IXF54_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1406292.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2818..4704
                     /locus_tag="IXF54_003134"
     CDS             2818..4704
                     /locus_tag="IXF54_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1406293.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4716..5663
                     /locus_tag="IXF54_003135"
     CDS             4716..5663
                     /locus_tag="IXF54_003135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1406294.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5683..6210
                     /locus_tag="IXF54_003136"
     CDS             5683..6210
                     /locus_tag="IXF54_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1406295.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6273..6926)
                     /locus_tag="IXF54_003137"
     CDS             complement(6273..6926)
                     /locus_tag="IXF54_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1406296.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7058..7900
                     /locus_tag="IXF54_003138"
     CDS             7058..7900
                     /locus_tag="IXF54_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1406297.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8058..8954
                     /locus_tag="IXF54_003139"
     CDS             8058..8954
                     /locus_tag="IXF54_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1406298.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8957..9670
                     /locus_tag="IXF54_003140"
     CDS             8957..9670
                     /locus_tag="IXF54_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1406299.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9690..10208
                     /gene="msrA"
                     /locus_tag="IXF54_003141"
     CDS             9690..10208
                     /gene="msrA"
                     /locus_tag="IXF54_003141"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1406300.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10205..10432
                     /locus_tag="IXF54_003142"
     CDS             10205..10432
                     /locus_tag="IXF54_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1406301.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10584..11135
                     /gene="lepB"
                     /locus_tag="IXF54_003143"
     CDS             10584..11135
                     /gene="lepB"
                     /locus_tag="IXF54_003143"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1406302.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11218..11760)
                     /locus_tag="IXF54_003144"
     CDS             complement(11218..11760)
                     /locus_tag="IXF54_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1406303.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11997..12395)
                     /locus_tag="IXF54_003145"
     CDS             complement(11997..12395)
                     /locus_tag="IXF54_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1406304.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12655..13515
                     /gene="ylqF"
                     /locus_tag="IXF54_003146"
     CDS             12655..13515
                     /gene="ylqF"
                     /locus_tag="IXF54_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1406305.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13508..14275
                     /locus_tag="IXF54_003147"
     CDS             13508..14275
                     /locus_tag="IXF54_003147"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1406306.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14331..15188
                     /gene="dprA"
                     /locus_tag="IXF54_003148"
     CDS             14331..15188
                     /gene="dprA"
                     /locus_tag="IXF54_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1406307.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15310..17388
                     /gene="topA"
                     /locus_tag="IXF54_003149"
     CDS             15310..17388
                     /gene="topA"
                     /locus_tag="IXF54_003149"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1406308.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17357..18733
                     /gene="trmFO"
                     /locus_tag="IXF54_003150"
     CDS             17357..18733
                     /gene="trmFO"
                     /locus_tag="IXF54_003150"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1406309.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18931..19836
                     /gene="xerC"
                     /locus_tag="IXF54_003151"
     CDS             18931..19836
                     /gene="xerC"
                     /locus_tag="IXF54_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1406310.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19873..20421
                     /gene="hslV"
                     /locus_tag="IXF54_003152"
     CDS             19873..20421
                     /gene="hslV"
                     /locus_tag="IXF54_003152"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1406311.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20435..21835
                     /gene="hslU"
                     /locus_tag="IXF54_003153"
     CDS             20435..21835
                     /gene="hslU"
                     /locus_tag="IXF54_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1406312.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21853..22644
                     /gene="codY"
                     /locus_tag="IXF54_003154"
     CDS             21853..22644
                     /gene="codY"
                     /locus_tag="IXF54_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1406313.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22726..23601
                     /locus_tag="IXF54_003155"
     CDS             22726..23601
                     /locus_tag="IXF54_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1406314.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8214 a         4093 c         4999 g         6429 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
     2701 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
     2761 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
     2821 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
     2881 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
     2941 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
     3001 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
     3061 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
     3121 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     3181 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     3241 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     3301 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     3361 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     3421 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     3481 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     3541 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     3601 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     3661 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     3721 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     3781 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     3841 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     3901 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     3961 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     4021 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     4081 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     4141 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     4201 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     4261 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     4321 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     4381 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     4441 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     4501 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     4561 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     4621 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     4681 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     4741 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     4801 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     4861 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     4921 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     4981 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     5041 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     5101 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     5161 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     5221 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     5281 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     5341 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     5401 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     5461 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     5521 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     5581 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     5641 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     5701 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     5761 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     5821 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     5881 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     5941 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     6001 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     6061 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     6121 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     6181 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     6241 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     6301 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     6361 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     6421 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     6481 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     6541 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     6601 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     6661 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     6721 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     6781 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     6841 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     6901 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     6961 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     7021 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     7081 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     7141 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     7201 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     7261 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     7321 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     7381 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     7441 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     7501 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     7561 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     7621 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     7681 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     7741 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     7801 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     7861 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     7921 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
     7981 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
     8041 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
     8101 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
     8161 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
     8221 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
     8281 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
     8341 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
     8401 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
     8461 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
     8521 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
     8581 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
     8641 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
     8701 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
     8761 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
     8821 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
     8881 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
     8941 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
     9001 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
     9061 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
     9121 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
     9181 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
     9241 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
     9301 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
     9361 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
     9421 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
     9481 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
     9541 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
     9601 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
     9661 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
     9721 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
     9781 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
     9841 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
     9901 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
     9961 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
    10021 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
    10081 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
    10141 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
    10201 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
    10261 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
    10321 tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga
    10381 tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt
    10441 tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt
    10501 atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa
    10561 aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc
    10621 ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat
    10681 tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg
    10741 ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag
    10801 atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa
    10861 atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga
    10921 aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac
    10981 aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag
    11041 atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct
    11101 attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt gcagtctgtt
    11161 gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta cgtaaaatca
    11221 aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat aaacaaccat
    11281 tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa ttactttgaa
    11341 atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga ctgggatcat
    11401 aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatgat ataaaaataa
    11461 gccattaata aatccaccaa ggaaagccgt gataagaaaa gcagcttgaa acgttcctga
    11521 accaaatatc gtgaaattta aagtgttcga caacatttca agcaaaccag cccagatcgg
    11581 tccatataga taacccataa gcgccatact taaaaatccg aatgtgattt gaaaataagc
    11641 aggaccaatc gctatcttgt ataagatgac atcaagggct aataaaagag cagaggtcga
    11701 caacaatctt gcattcattt cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa
    11761 aatattctct cctttgtcag ttaataataa aaggatacca taaaatcacc aatgtgtcat
    11821 gacacattgg tgatttacat acactggcat ctaacaatta aaaaaagggt gagaagactg
    11881 cttttataaa ctgtaaaaaa gagattgtac gaataaaaaa aatagaggcg gagaatactt
    11941 atcgtatcat ccgcctttgt ttttttgtta taaaaaacgt cagattagtt atctgcttac
    12001 atcttcatcc atacgggttc taatgtattt ccgtctagat cttggacttc taatgaaaac
    12061 atttcttctt caggcattcc catatctact tcgtggaaag taccaccatt ttcttttgca
    12121 atttgagcaa atttttttac ttcatccgca ttttcaacac taaacgaaat caaagcacca
    12181 cttgtttttt tattatcagc tatttcttta tcttttataa attttttata aaaatcatga
    12241 gagagaagca ttatccaaaa agtgtcatcc cataccattg aaccagcttc ttcagtagaa
    12301 aattcttcat tttttttaaa acccagcttt tcataaaaag caacggaacg attgacatca
    12361 gcaacaggaa aattaacaaa aaccatacta ttcatttgtt tcgtctcctt tctgttttgt
    12421 tcgtctatca tctcttattt aggaaacgct gtcaaaaatt tagattcata tatagaaacc
    12481 aaaataaaaa tttgcatcat caaaaaagtg aagatgtgat agaatgagga ctggttcact
    12541 ttcacttaac taatagatgg aatttcttta taataaaaaa gtgaatttat attgaaaaag
    12601 aaaaaaacaa gtaaagccgg ggaatattcc ggtatgaaag gaatggagag aacaatgacc
    12661 atccaatggt ttccgggaca tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa
    12721 tatgtggaca tcgtatttga attggtagac gcacgattac ctttatcttc tcgtaatcca
    12781 atgctagatc agatcattca gcaaaaacct cgccttgttt tattgaataa agcggatctg
    12841 gcagatccac aacagacaaa gctgtggcaa gaatattttc gtaaacaagg ccatcttgca
    12901 ttagctatca cggcacaaga aagtaaagga attaaaggtc tagtacctgc ggctaaagaa
    12961 gcattgaaag aaaaacgggc aagagatgct gctcggggga tcaagcctcg cgctattcgg
    13021 gcaatggtct taggtattcc aaatgttggg aaatctacgt tgatgaatcg actagtaggc
    13081 aagaaaattg cacagacagg aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt
    13141 ttaggtaatg agttggaatt gttagacaca ccagggattt tatggcccaa atttgaagat
    13201 gaagaaatcg gcaaaaaact agcgctgaca ggcgcgatca aagaccagct tgtccattta
    13261 gatgatttag cgatttacgg attagacttt tttgcccgat attattcagg aagaatcagc
    13321 gatcgttatg gtttatcaat cgagcaagaa gaaaaattgg caccggaatt actgatggat
    13381 attacagcaa aacgtgggta taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat
    13441 gatattcgtc aaggaaaact tggacgatac acactggatc gttgcgaaga aatggagcaa
    13501 aaaagtgatg actgaatcca tccaaagtat caaagaacga ttgaaaagca tacagagaat
    13561 agatgatcct acactagaat cattaagaaa tgatccacgc aaaggcgtgc agcaagcact
    13621 ggcttctttt gaacgacgat tgaaaaagga agcattaatt aaggaaaaat atgaagaaat
    13681 gttttgcttt gaaaagcaag ggtacgcaca aggtcaccaa tggattgccg gcattgatga
    13741 agtaggaaga ggaccattag ctggacctgt cgtggcagct gcagttatct tgccgaaaaa
    13801 tgagatcata ttagggctga atgactccaa acaattatca gagaaaaagc gagaacttct
    13861 ttatcaacaa gttcaagaaa aagccctggc aatcgggatc gggatcgttg atcagcagac
    13921 aatcgatgat gtgaatattt atgaagcaag taaattagcg atgatccaag cagtagaaaa
    13981 attgtccatt gtaccaagct atttgttgat cgatgcgatg accttggatt tgcctattca
    14041 gcaggaaaaa ataatcaaag gggatgcacg atctgtgtcg attgctgctg caagtatcat
    14101 tgctaaagta tatcgagacc aattgatgaa agaatatgac gtgctttatc ctggatatgg
    14161 tttctcgcat aatgcaggtt atggaacaaa ggaacactta gaaggacttg aacgacaagg
    14221 aattacgcca atccatcgta aaagttttgc acctgtaaaa aaatatttac aataaaaaac
    14281 agccgctttt acgtatctta ttagaaagag agacgaaagg cggttttatg atgtatcaaa
    14341 tagaagaaaa tttattgaaa cttgcttata caaaaggaat cagtagtcaa ggaaaatggg
    14401 cacttgctaa ctggatgatg cagtatcctt ataaagaaat cggatttgaa gacatcatta
    14461 agataggtaa aataaccaaa caccgggagc tgtttcgcaa aagttggcag gaaattcacg
    14521 ctaactgggg caaaatccaa tcgaaacagt cgtttatcac ttgctttgat cccaaatatc
    14581 cccctcagct gcttcacttg acctatccgc caattgtact gttttataat ggtgatctat
    14641 ctttactttc ttgtaacatg ttaagcgttg ttggtggaag acacacttct gctttagcca
    14701 aaaaaacagt ttattattta ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg
    14761 gctgcgcgaa agggatagat acatacggtc accaagcagc aatctctcat ggtggacgga
    14821 caatcgcagt cattggaaca gggatagagc aagtctatcc tgctgaaaat cgtgacttgc
    14881 aagaggtgat tgcaaatgat catcttcttt tgagtgaata tcctcctcaa tccggcccga
    14941 aaaagcattg ttttccaatg agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg
    15001 tctttgaagc caagaaaaaa agcggctctc tgataacagc acagcaagca ttggatcttg
    15061 gaagaacagt tttttctgtt ccaggaaata ttttaacaga acattctacc ggatgtcatc
    15121 atttgatcca agacggtgca gtttgcacgg tttcgggtga agatatcttg gcagaattta
    15181 aagaataaca ccatactttt catttgcttt tcttgacaaa cgttttctga atggatatga
    15241 ttagctacga tttgtatgaa tcctctttta atagtaggaa cacgacaatg gaaaggagtc
    15301 cattcagtaa tggcgtataa atatttagtg attgtagaat cacctgcaaa agccaagaca
    15361 attgagaaat atcttggacg aaactataaa gtaatggcca gtgttggcca tataagagat
    15421 ttaccgaaaa gtaaaatggg tatcgatttt gaaaacaatt atgaacccca ttatatttct
    15481 atacgcggaa aaggcgatgt catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa
    15541 gtttacttgg caagtgaccc ggatagagaa ggagaagcga ttgcttggca tttagcgtac
    15601 ctacttgggt tggatctgaa agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac
    15661 gcagtcaaag cagcttttaa ggaaccaaga acgatcgatg tagatttagt agatgcacag
    15721 caagctcgtc gtaccttaga cagaatcgtt ggttattcga tcagtcctat tctctggcgt
    15781 aaggtcaaga aagggttaag tgcaggacgt gtccaatctg tcgctttaaa aattattatt
    15841 gaccgtgaaa aagagatccg agaatttgtt ccagaagaat attggagcat cgacggtaat
    15901 tttaaaaaag ctcgcaagaa attcaaagca aatttctggg gaatcgacgg taagaaaaag
    15961 aaattaccag atgcacaaag tgtaaaagaa gtcactgcta gaattgatgg aacagacttc
    16021 gaagttaaaa aagtagaaaa gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca
    16081 agtagcctgc aacaagaagc tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg
    16141 gtagcacagc aattatatga aggaatcact ttgggtaaac aaggaacagt cggcttgatt
    16201 acatacatgc gtactgattc tacccggatt gccgatacag caaaagcaga ggccgcagca
    16261 tttattgaag atacgtatgg aaaagaattt tctagacatg atcaccgaaa gatcaaaaat
    16321 gctcaaggcg cacaagatgc ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca
    16381 gatgaactca aacaatattt agataaagat caattgaaac tttatacatt gatctggtca
    16441 cgttttgtgg ctagtcagat gacaccagct attttagata caatgaaagt gacattagaa
    16501 caaaatggtg tcatcttcat tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa
    16561 gtgtatgtgg aaggtagaga tgacggcaaa gaagagaaag aaaatattct tccagaactt
    16621 gaagaaggcg atgtagtcca atctgttgat attgaaccaa aacaacattt tactcaacca
    16681 ccagcacgtt ttagtgaggc tacgttgatt cgtgcactag aagaaaacgg ggtagggcga
    16741 ccttccactt atgcaccaac acttgatacg atccaaagac gctactatgt aaaattgaca
    16801 caaaaaagat ttgagccgac tgaactagga gaaattgtca attcattaat ctgtgaattc
    16861 ttccctcaaa tcgtcgatat ccatttcaca gcagaaatgg aaggagattt ggataaaatt
    16921 gaagaaggca cggaagcgtg ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa
    16981 gaactgacga atgcggaaga aaaaatcgaa aaaattcaaa tcaaagatga accagcagga
    17041 ttcgattgtg atgtctgtgg acatccgatg gtgataaaat tagggaaata tgggaagttt
    17101 tatgcttgca gtaattttcc tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt
    17161 gtgacttgcc cagtatgtca tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga
    17221 ctattctatg gttgttcccg tttcccagaa tgtgaattca cttcttggga taaaccagtg
    17281 ggcagaaatt gtccaaaatg tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa
    17341 caagtagtct gtgtaaatgg agattacgaa gaagacatcc aaaaataata gaaaagaggt
    17401 tctttcattg aaaatggtaa atgttgttgg agcagggctt gccggaagtg aagctgcttg
    17461 gcagatcgca caagcaggag tccctgtccg tttatatgaa atgcgtccag tcaaatcgac
    17521 tgaggcgcat cacacaagta attttgctga actagtatgt tccaattcat taagaggaaa
    17581 cagtttggct aatgcagtag ggttattgaa agaagaaatg cgccgattga attcggtagt
    17641 aatcacttcg gcagatgaaa cagcagtgcc agctggtggt gccttagctg ttgaccgcga
    17701 ttctttttct gaaacaatta cccgaaaagt caaagagcat ccattagtaa cagtgataaa
    17761 cgaagaaatt actgaaatac cggaaggaat caccgtagta gcaacaggac ctttgacttc
    17821 tcatccatta gcagaaagca tcaaagcatt caatggttca gatggttttt atttctatga
    17881 tgcagctgca ccaatcgtag acaaagctac gatcgatatg gataaagtat atttgaagtc
    17941 ccgctatgac aaaggagaag cagcttactt gaactgtccg atgacagaag aggaattcaa
    18001 agctttccat gaggcactaa tcaatgccga agtggcagaa cttaaatctt ttgagaaaga
    18061 aaaatatttt gaaggatgta tgccaattga agtaatggca cagcgaggat tcaaaacgat
    18121 gcttttcgga cctatgaaac cagttggatt ggaagatcct aaaactggca aacgtcctta
    18181 tgctgttatc cagctgcgtc aagacaatgc tgcagcttct ttatacaaca tcgtgggctt
    18241 tcagactcac ttgaaatggg gcgagcaaaa acgtgttttc cggatgattc ctgggttaga
    18301 aaatgctgaa ttcgttcgct atggtgtgat gcaccgaaac agctttatga attcgccaga
    18361 attgctgaaa ccgacttacc aatcaacgaa acgcgaagat ctgttctttg caggtcagat
    18421 gactggtgta gaaggctatg ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc
    18481 cgcacgctta gcaaaagaaa tggaaccagt cgagtttcca caagaaacag ctattggaag
    18541 tatggcttac tacattactc atgcggaagg aaaacatttc caaccgatga atgccaacta
    18601 tggtctgttt cctgaactgc cagagcgcat tcgagacaaa aaagcaagat atgaagcaat
    18661 tgcacaacga gctttaaaag ctaatgaggc aataaaaaca gaattagaaa aaactgcaat
    18721 cccaaatgag taaggaaact ccatgaagag ggtgagacgg atagtctcgc tctttttctt
    18781 gcgtttttct taagaatttc tcaaacaaaa tgtgaggaat tgcgccaatt gtgaacaaac
    18841 ttagaaatat tggttaaatt ctgacaattt atttgtcttt ttataaacgc tgtgctaaat
    18901 tattctctgg gacaaaagga aggtgagaaa atgacagaag ccacgtggga acaaaaattt
    18961 ttgcgttatt tgattgtcga aagaggatat tcagaaaaaa cgaaagaagc atatgaagaa
    19021 gacattctcc atttcaaaca ttttttggag gaatctggag acgctgatct gctgaaggcg
    19081 gaatatttta cggttcgtgt ctatttgagt gcattagctg atcatcgtcc tagctatagc
    19141 agaaattcta tcagtcgcaa gatagccagc ctacgttcgt tttatcagta tcttctcaaa
    19201 gaagaagtga taaaagaaaa tccattttct tacgttcatc tcaaaaagaa aaatttgaga
    19261 ctgccacgtt ttttttatga aaatgaaatg caagcacttt ttgacagcgt agcaggagat
    19321 agtcctttgg atttgaggaa ccgcgcttta ctggaagtcc tttacgggtc aggcatccgt
    19381 ttaagtgagt gtagcagttt gtcggtatca gacattgatt ttgattcaga ggtcatgttg
    19441 atccatggta aagggaacaa agagagatat gctcctctag gttcttttgc tcaagatgct
    19501 ctgcaagaat atttcaggga aggccgccag gtattgatga caaaatatca cgaagagcat
    19561 gatatcgttt ttgtgaatca tcatggaaaa ccaatcacgc ctactggaat tgaatatgtc
    19621 ttgaatcaaa tgatcaaaaa aagcagtttg gatagtgata tccatcctca catgctgcgc
    19681 catacatttg ctactcattt attaaataat ggggcagatt tgcggacagt ccaagaattg
    19741 ctaggacatg ccaatttatc gactacccag atctatgccc atgtaacaaa agaaagttta
    19801 caaaaaaact atcgttcttt ccatccaaga gcgtaaaaat aaggaaaaga acaaatttag
    19861 gaggaaacaa acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa
    19921 gacgggaaat ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg
    19981 aaaggcactg caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc
    20041 ggaagtgttg cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat
    20101 aacgggaatc tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct
    20161 atgcaaaaac ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca
    20221 ggtacaggag aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat
    20281 tttgcgttgg ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa
    20341 gaaattgcag aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat
    20401 attatcgtag aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag
    20461 acaaatcgta agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt
    20521 tgccgttgct ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga
    20581 tattacacca aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc
    20641 acgacgtttg gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac
    20701 tgaagtcggt tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc
    20761 gatccaaatc gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc
    20821 agaaaaacgt ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac
    20881 taatcaattt gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc
    20941 gcaagaagaa gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca
    21001 aaagggttta ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc
    21061 accaatgatg aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc
    21121 tttgacaccg aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt
    21181 gatcaaggaa gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa
    21241 attagcggaa gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc
    21301 acagcaatct ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga
    21361 aggttcacaa gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc
    21421 ttcaggagct ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt
    21481 cccgatacgg gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga
    21541 accaaataat gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt
    21601 tacgtttaca aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga
    21661 cacagataat atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct
    21721 attatttgaa gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga
    21781 tgacaaactt ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat
    21841 cggaggacga caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag
    21901 caaaagaata cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta
    21961 ggtggtattc ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat
    22021 aatgaacgtc acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt
    22081 cctgaatctt atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt
    22141 actagtgata ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg
    22201 actgtcgttc cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg
    22261 gaacaatcgt ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt
    22321 atgcagatcc tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct
    22381 gtccaaatgg caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt
    22441 gaagcattgg atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc
    22501 atcactcgtt ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa
    22561 tctcgttcgt taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac
    22621 gagttagcaa aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc
    22681 cagccttccc attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt
    22741 caaaatgacc agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa
    22801 tcaaaagaac atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat
    22861 gcaccggttt tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg
    22921 aaaacatatc aaatggggca acacggattt gctcgggata tggattttga agtcattgaa
    22981 catggacaag aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat
    23041 ccatttgatt ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat
    23101 tatcaagtgg caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct
    23161 ttcaatgtac ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca
    23221 ttgaaatcaa gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg
    23281 ttaggtcaga caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg
    23341 gtttttgaaa caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc
    23401 gtaactttaa gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa
    23461 gcgcctttcg tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga
    23521 aacttgatgg aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa
    23581 tattccatca cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac
    23641 caaggtgtgt aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtggct
    23701 ctttgtcaat aaggactgat gagttgtgca aaatc
//
LOCUS       DACERL010000348        20985 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.051, whole genome shotgun sequence.
ACCESSION   DACERL010000348 DACERL010000000
VERSION     DACERL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.051"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(134..577)
                     /locus_tag="IXF54_003156"
     CDS             complement(134..577)
                     /locus_tag="IXF54_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1406315.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IXF54_003157"
     CDS             complement(795..2135)
                     /locus_tag="IXF54_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1406316.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IXF54_003158"
     CDS             2404..3300
                     /locus_tag="IXF54_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1406317.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IXF54_003159"
     CDS             complement(3450..4391)
                     /locus_tag="IXF54_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1406318.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IXF54_003160"
     CDS             4517..5638
                     /locus_tag="IXF54_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1406319.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IXF54_003161"
     CDS             5896..7398
                     /locus_tag="IXF54_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1406320.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IXF54_003162"
     CDS             7465..7992
                     /locus_tag="IXF54_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1406321.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IXF54_003163"
     CDS             8203..9264
                     /locus_tag="IXF54_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1406322.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IXF54_003164"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IXF54_003164"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1406323.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IXF54_003165"
     CDS             10177..12159
                     /locus_tag="IXF54_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1406324.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXF54_003166"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXF54_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1406325.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IXF54_003167"
     CDS             12881..14305
                     /locus_tag="IXF54_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1406326.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IXF54_003168"
     CDS             complement(14486..15259)
                     /locus_tag="IXF54_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1406327.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IXF54_003169"
     CDS             complement(15256..16161)
                     /locus_tag="IXF54_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1406328.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IXF54_003170"
     CDS             complement(16315..16635)
                     /locus_tag="IXF54_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1406329.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IXF54_003171"
     CDS             17109..17795
                     /locus_tag="IXF54_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1406330.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IXF54_003172"
     CDS             17797..18741
                     /locus_tag="IXF54_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1406331.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IXF54_003173"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IXF54_003173"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1406332.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IXF54_003174"
     CDS             19663..20940
                     /locus_tag="IXF54_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406333.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3785 c         4359 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcattatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttcccgc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttatga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACERL010000349        20416 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.054, whole genome shotgun sequence.
ACCESSION   DACERL010000349 DACERL010000000
VERSION     DACERL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20416
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.054"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(565..792)
                     /locus_tag="IXF54_003175"
     CDS             complement(565..792)
                     /locus_tag="IXF54_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1406334.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IXF54_003176"
     CDS             complement(801..1274)
                     /locus_tag="IXF54_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1406335.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IXF54_003177"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IXF54_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1406336.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IXF54_003178"
     CDS             3083..3271
                     /locus_tag="IXF54_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1406337.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IXF54_003179"
     CDS             3284..3841
                     /locus_tag="IXF54_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1406338.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IXF54_003180"
     CDS             3873..4115
                     /locus_tag="IXF54_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1406339.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IXF54_003181"
     CDS             4456..5670
                     /locus_tag="IXF54_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1406340.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IXF54_003182"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IXF54_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IXF54_003183"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IXF54_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1406341.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6938..7594
                     /locus_tag="IXF54_003184"
     CDS             6938..7594
                     /locus_tag="IXF54_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1406342.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7776..8306
                     /locus_tag="IXF54_003185"
     CDS             7776..8306
                     /locus_tag="IXF54_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1406343.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8852..9094
                     /locus_tag="IXF54_003186"
     CDS             8852..9094
                     /locus_tag="IXF54_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1406344.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9091..9561
                     /locus_tag="IXF54_003187"
     CDS             9091..9561
                     /locus_tag="IXF54_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1406345.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9689..10903
                     /locus_tag="IXF54_003188"
     CDS             9689..10903
                     /locus_tag="IXF54_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1406346.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(11613..11801)
                     /locus_tag="IXF54_003189"
     CDS             complement(11613..11801)
                     /locus_tag="IXF54_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406347.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            11892..12101
                     /locus_tag="IXF54_003190"
     CDS             11892..12101
                     /locus_tag="IXF54_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1406348.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12213..13826
                     /locus_tag="IXF54_003191"
     CDS             12213..13826
                     /locus_tag="IXF54_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1406349.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13861..15072
                     /locus_tag="IXF54_003192"
                     /pseudo
     CDS             13861..15072
                     /locus_tag="IXF54_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15095..16021
                     /gene="glsA"
                     /locus_tag="IXF54_003193"
     CDS             15095..16021
                     /gene="glsA"
                     /locus_tag="IXF54_003193"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1406350.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16229..17689
                     /locus_tag="IXF54_003194"
     CDS             16229..17689
                     /locus_tag="IXF54_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406351.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17910..19673
                     /locus_tag="IXF54_003195"
     CDS             17910..19673
                     /locus_tag="IXF54_003195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1406352.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19757..20215
                     /locus_tag="IXF54_003196"
     CDS             19757..20215
                     /locus_tag="IXF54_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1406353.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20324..>20416
                     /locus_tag="IXF54_003197"
     CDS             20324..>20416
                     /locus_tag="IXF54_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1406354.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGR"
BASE COUNT         7083 a         3129 c         4174 g         6030 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aagacaaatt ccagtttgta
     6901 tggggcgata aaatcagaat ttgagagagg attgaatatg aataaggaat ggtctgaact
     6961 taataaaaca atgcaggcac aaataaaaaa gaaagatact tataagaggg gtattgatac
     7021 tttacttact ttacgaagtc agttaataca aaccttagta tctttcaaag aagaattatg
     7081 cagagaagat tttaactcaa tccccttcat aaacgctgat ggttatcata gtaagacgat
     7141 tgcttattct atttggcata ttttccgcat tgaagatata gttgtgcata cagtgataaa
     7201 tgaagatgaa caagtatttt ttgcaggcaa ttatcaagag cgtatcaatt cacctatcat
     7261 tacaacagga aatgaactca tgaaacagca gattgcagac ttttcaaaac agcttaatct
     7321 ggaagaatta tatttatata ttttcgaagt atgggaaagc actgagaaaa tgcttgaacg
     7381 gttatcttat gatgaattga aaagaaaaat accgaaagaa agaaaaggat acttagaatc
     7441 gttgaatgta gtaaacgaca atgaaaaagc aatttggctg attgattatt ggtgtaataa
     7501 agatatttgt ggactaattc aaatgccgtt ttcaagacat tggattatgc acacagaagc
     7561 ctgtttgcgt ataaagaaca agatacattc ataggcaaaa atttcagttt gtagaactaa
     7621 ggaaattgga atttaaggag gaataaagta tggttagtga ttttataaga gcagcatttc
     7681 catggatttt aatgggttta tttgtagcaa tcagttgttc ccttatgagt aaaaaagaga
     7741 aataaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt ttatttattg
     7801 ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg ctaaatggtc
     7861 atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat atagatactg
     7921 tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg caagaatggg
     7981 tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct tcccaacaaa
     8041 ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa gaagcaggaa
     8101 aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc aaaaatatga
     8161 ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt cgtcttttgg
     8221 gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat aacggaataa
     8281 ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt aaaccgacgg
     8341 agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc ttgcaagggg
     8401 gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg ttatctaaca
     8461 aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc tgtatttata
     8521 aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact atgaaaattg
     8581 aatagtaaat atgagaaaga cagttgattt caaaaaataa gaaattgatt gtcgttttct
     8641 tttgcagaaa tttaagtgca ggcaattatg gtagttgtcg taattgctgt cagctcagtg
     8701 tttatcactg atggtgtgaa agatttacgg ttggagatgt tcaataagat tattggtgct
     8761 taattatttt tattgtagtg ctgatatgag aatgttataa ttggggtata aaacatacaa
     8821 ataaatttgc tgaaaaatga ggtatcaaaa tatggaaaat gaattactgg aaaacttagg
     8881 aagacttcac acaactgaac ttggcgttat tagaattaaa aagaacttat ctttgaatgt
     8941 agaaaatgta attgaatggt gcaaagaaaa aattagcctt tctaatgcgg aaatcacaag
     9001 aaaaggaaaa aactggtata taacgattga taattgtatt ataacggtaa atgcgtacag
     9061 ttataccgtt atcactgctc acaaggtaaa atgataaatt tttctataaa ggaattaaac
     9121 gcatttgcag ttattgggca ggaagtagaa ctgactaatt atcaaaaaaa gaatattcag
     9181 attagtacac agttttggag aaagtttaac agtagtttga aaaaagcgta tctttcacaa
     9241 tcaggaaatt gggtaaaata tgcttttatg gaaagaagaa atggaaaact ttattatttc
     9301 tgttctattc cccaaagaac cattacccca gagggctttc tgtataaaga aataccgtct
     9361 tataaatatt tggttgtgga gcatattggt gctatggata aaatatatga aacttatggc
     9421 aagatttatg aagaaattat tcctagtaca tcgtatattc ctataaaaga tattatccta
     9481 cactttgaaa aatacgatta tcgttttcat tggaatagtg ataattcagt tattgaaatt
     9541 tggataccta ttcaagatta gaagcaattt tatatccata cacacaaagc agttagtctt
     9601 aggattgact gctttttcta tacagatttt taatgacagg caattatcaa ttatggtagt
     9661 tgcctttttt gattggagga attgaagaat gaatgataaa aactttattg aagaactaag
     9721 acaaaagcgt gaggaatacg gagtaacaca aacaaggctt gctgttgcct gtggtatcag
     9781 ccgtgaatat tacaaccgca ttgaaaaagg aaaacagcca ttgaatgacg aattaagaga
     9841 aatcatagaa aaacagattg agcgtttcaa tccgcaagaa ccactattct tattgattga
     9901 ttactttcgt gtccgctttc ctactacgga cgcattggcg attatccgtg atgtattaca
     9961 actgaaatct aattacatgc tttatgaaga ttatggaaaa tacggatatg aaagcaaata
    10021 tgtacttggc gacatcaata tcatgtgttc catgcaggag catttaggtg ttttattgga
    10081 actgaaaggc aaaggctgtc ggcaaatgga atgttatctg ctggcacagg aacgctcatg
    10141 gtatgacttc atgctggatt gtatgacggc tggtggtgtg atgaaacggc ttgatttggc
    10201 tatcaatgat aaagcaggaa tattggatat tccaaagtta aaggaaaaat acaaggctgg
    10261 cgaatgtatg tcctacttcc gtatgcagaa agattacagc ggtacagaaa aatgcggtag
    10321 cgatttacca aagaatacag gagaaacctt atatctcggt tcaacaagta gcgaattata
    10381 tatgtgtgct tatcagaaaa attatgagca gtatgtcaag aatggcacag aaattgaaga
    10441 tacggagatt aagaaccgtt ttgaaatacg catgaagaac gaacgagcct attatgcggt
    10501 tgtagattta ctgacctatc gggacgctga acgcaccgct ttttctatca tcaatcatta
    10561 tgtccgcttt gttgacagag aggacgacaa gccaaaaagt caatggataa caaatgatga
    10621 ttgggcatgg tttgtagggg aaaacagaga gccgatacgc ttaaccacaa agccagagcc
    10681 ttacacttta caaaaagcgt tgcattggct acaaagacaa gtcgcaccaa ccataaaaat
    10741 ggtacaggca ttagacagag aaaatcgtac aacgatactg aaagatatga ttgagcaggc
    10801 agaactaaaa gacaagcata aacatttatt acaattagag aaatcaacca tagaagaacg
    10861 tatagatacc gctgttccac aagagaatga cggtattttt taatgtaatt gaaaaatttc
    10921 agatttagtc agcaaagagc tgttttcatt ccctatgtga agtgaaagaa gaaaaataaa
    10981 ttttttatta aatctgcaac aaaatggcta cttgcgtaca gatatggtgc gagaaaagga
    11041 aatctaaact ttttttgaaa ataggtcatt aaatcggggc ttgctgtccc tatatgaagt
    11101 gaaagaagaa aaaagttttg aaaaaatgac ggaaattcgt gattagtgga cagtcttctg
    11161 tgaaaagagg aaaaacatca tcattttgta ttcaaacagg cattagcagt tgagtgttga
    11221 tgtgaaaaga tgaaaatact ttgttctcaa cttaacaatt taaggtttcc gttccctata
    11281 tggtgcaaag aaaatatttc attctattgt tggcagtaac ggtatggttt gggtagttag
    11341 ggtgtgaaaa ggaaaatatt cttttttcat catcaactta gcaaaatgac attttctatc
    11401 cctatatggt gcatgaaaag aagttaaaat ttttccattt aaacttagca aatggacgtt
    11461 tgctgtacag atagtagtga aaaaggagaa aatcaaccgt tgattgccaa aatgaaccgt
    11521 tccatttgta ctaataatgg aaaaagtttt ggattttggt tacgtttctc ctcttttcca
    11581 acgcgatata taggaaagac gatttttttc tttcaatcgt tctttgacaa ctgaataaag
    11641 ctattcaata cgtttgacag acagctagcc gttgaaacaa atacgccacg acctttcccc
    11701 tgcaagagcg agcgaaaacc tatttgtgtt cttgtaaaag atttgtcctc ttttatcgcc
    11761 atgacctgta ctgtcagata atgatactcc cgtacagcca cagcttgagc attaagagcg
    11821 tcgcacgcaa tgggtacggc tacataagaa ctatgcagag gtggaactcc tgtgggctat
    11881 gacaaaagcc gttgaattgc ttagaaagat tttacagaaa gggggtatgt gttattgata
    11941 atgctgtaaa aacaattcaa aagaaaaatg gcaagtgtgg cattggcaac agaggaaaga
    12001 caaagattgt agtaaaagag catttttccg aaaagggcaa aacaatggaa gaacttctaa
    12061 ctgatgtcat gctggaaaaa gcaaagcaga caatcgcata aaatcggtgc agttgtaaga
    12121 aatagattga atgacaaaaa gtacccgtgt tatactttac ttgcaagcag gtattgtaaa
    12181 cacgggttag ttataaagga ggataactga caatgaaaca acagatttac aatactgcac
    12241 tttatcttag gttaagccga gatgatgaat tacagggcga aagttccagc attaccacac
    12301 agagaagtat gttgcgttta tatgcaaaag aacatcattt gaatgtcatt gatgaatata
    12361 ttgatgacgg ctggtcggga acaaattttg acagaccgag ttttcaaaga atgattgagg
    12421 atatagaggc aggaaaaatc aactgtgttg taacgaaaga cctttcccgt cttggcagaa
    12481 attatattat gacaggacaa tatacagaat tgtatttccc tagtcataat gtccgctaca
    12541 tagcgattga tgacggtgta gacagcgaaa aaggcgaaag tgagattgca ccatttaaga
    12601 acatcatcaa tgaatgggtg gcaagagata caagccgtaa agtcaaatca gcatttaaga
    12661 caaagtttgc ggagggggct cattacggtg cttatgctcc gttaggatat aagaaacacc
    12721 ctgacatcaa aggaaaactg ttggttgatg atgaaacaaa atggattatt gaaaaaatct
    12781 tttccctagc ctatcaaggt tacggaagtg caaaaatcac aaagcagtta agagcagaaa
    12841 aagttccgac agcgtcatgg ctaaatttta caagatacgg tacttttgca catatctttg
    12901 agggaaaacc cgaaagtaag cgttatgagt ggacgattgc tcatgtcaag gcgatattga
    12961 aaagtgaggt ttatatcgga aacagtgttc acaatatgca gtctacggta tcgttcaaga
    13021 gcaaaaagaa agtgcgtaag cccgaaagtg aatggtttcg agtagaaaac actcacgagc
    13081 cgattattga caaggaagtg ttctatcgtg tgcaggagca gataaaatca agacgcagac
    13141 agacaaagga aaaggcaacg ccgatttttg cagggcttgt caagtgtgcg gattgtggtt
    13201 ggtctatgag atttggaacg aataagacaa ataaaacgcc ttacagttat tatgcttgca
    13261 gttactacgg gcagtttggc aaaggtaatt gttctatgca ctacattcgt tatgatgtgc
    13321 tgtatcaagc cgtattggaa cgcttgcagt attgggctaa ggcagtacag caggacgaag
    13381 aaaaggtatt gaacaagata cagaaagctg gcaatgcaga gagaatacgg gaaaagaaga
    13441 aaaaggcaag tacattgaag aaagccgaga acagacaaaa tgagattgac cgtttatttg
    13501 cgaaaatgta tgaagataga gcctgtgaga agataacaga gcgaaatttt gtgatgttgt
    13561 ccagcaagta ccaaaaagaa cagatagaac tggaacagca gataacaagc ctaagagaag
    13621 aactaagtaa aatggaacag gatatgatag gtgctgaaaa gtggattgag ttaatcaagg
    13681 aatattccgt accaaaggaa ctgacagcac cattattaaa tgccatgata gaaaaaatcc
    13741 tcatacatga agcaacaacg aatgaggata acgaaagaat acaggaaatt gagatatatt
    13801 accgatttat tggaagagtt gagtaatcaa caagagtaat atttttaact aagggaaacc
    13861 ggcggtgtct atacttgggt cagtcgcaca ttgggaaaac gattcggttt tgccgcaatc
    13921 tttttccaat ggtttgagat aacagttggc tacttgacca tgctgtattt cttaactggt
    13981 gcattatcct atgcaacggg tattggtgct atacaaaaca ataaatatct gaaattggca
    14041 atcctattga ttattttctg ggctatttta ctctcacagt taaaaggaac caaatacact
    14101 tcattgattg ctcggatcgg ttttgtagct ggtatcttgt tgccagcatt agtcttgttt
    14161 ggcttaggga tccattatgt cgcttcaggc gctccactac aaacgatact gtctatgaaa
    14221 acattgattc ctgatttttc gaaattgcct acgctggttg tttttgtttc ctttatttta
    14281 gcatatatgg gcgtagaaac atctgccagt catgcaaatg aaatggaaaa ccctaaaaag
    14341 gattatccat tagcaatgtt tgtattggtt attctggcaa tcattttaga tacttttggc
    14401 ggattgaccg tcgctactac agtacctcaa catggtttgt ctcttaatac aggggtgatt
    14461 caatcgttag aattcctatt aagacacatt gatccaaatc ttgcatggat tgcgaaaatt
    14521 cttgcgattc ttgtttgttt aggtgtcatc ggagaaattg cttcttgggt aacctctcct
    14581 tcaaaagcac ttcacgtagc agcagaagaa ggattacttc ctgaatattt tgcaaaagaa
    14641 aacacacacg gtgttcctgc tcatttgatg atcgcaaatg ggatcgttgc aacaatctgg
    14701 gcagccgtct ttacattaag cggcgggggg aataatcttt catttcttgc agcgatgtca
    14761 ttgactgttg taatctacct gatgttgtac tttatgtttt tcgcgggcta tttgaagctg
    14821 gtttttaaag atgacagtct tgaaagagcc tatcaggttc caggcggaaa agttgtaaaa
    14881 gtaatcgttg cttgtttagg tcttttgact tcactttttg ctttttgtat ctcatttgtt
    14941 ccacctgcta gtatcgcagc tggacagcat gggatttatg aaacgattct gattgtttct
    15001 tatcttatcg tcttagttat tccatttacg atctacgcat gccgtcataa attccagcat
    15061 gcagaaaaat aaaaaatgaa agctggttat tcaaatggaa gtaaaagaat tagaacaatt
    15121 gattggaaaa aatttccact attatcaaac aggacaagtg gcggattaca ttccagcatt
    15181 agctgaagtg gatcccaaac aattaggtat ggcgatttat gatttggata caaaacaact
    15241 gattgaggga ggagacgccg atgtgcgctt tgccatcgaa agcatgtcaa aagttcccgt
    15301 attgttatta gcaatcaaag acaacggaat agaaaaagtc tttcaaacaa tcaacacgga
    15361 accaaccggt tttgccttca attctatcat gaatatggaa atcaatcatc gcaaacatcc
    15421 aatgaatcct tttgttaatg caggagcgat tgcaacaacc tcgttgattg ccggtaaaga
    15481 tgcagatgaa aaatttgagc gaatcttgtc atttatgaaa gagatctgtg atgatcctaa
    15541 tatcacattg aatgaagaaa tctatcattc tgaatcaaga acaggtgata tcaatcgctc
    15601 tcttgcttac tatatgaaag gtaatcaaat gatcgaagga gacgtaccgg agatactcga
    15661 tgcttatttc agacaatgct ccctcaatgt caccgctaca ggcatagcaa aattagctgc
    15721 tgtgctggca aataaaggaa ttgctccttg gaatggcaaa caaatcatta ctgaagaaag
    15781 tgctacaatc gtcaaatcaa ttatgacaac tgctggtttg tatgatgaat caggagagtt
    15841 ttccgtccat gttggtgtac ctgcaaaaag cggtgtaggc ggcgggttga tggcagctgt
    15901 gccaaatcgt tacggcatag gtgtcttcag cccggcacta gatccatttg gtaacagcgc
    15961 agctggtatc caattgttga aagatgtagt caaagaacta gacgctgaca tctttgaata
    16021 accattcttg aaagaggctg gggtaaaagt ccaaacggct caaagctacc tacaatgagg
    16081 ctgaaattgt gagcttgttt gacttttgtc ccagtttctt tttttattgg tttttgaaag
    16141 ggcttttcta aataaaaagg aaacaaaaat acgatttaca gatatgatta aataaaatca
    16201 taagaaaaac tggaggcgtg tatcaggaat gctggaaaat tacatcgagc gaaatatatt
    16261 tagaaaagtg tatctgtgtg aacaattatt tgaattccaa gaaatagaca tcgaacaaac
    16321 agctatttct ctaagggtga ctacaccaac gatccttcac gatctagaaa gtctagccga
    16381 gtgtctagaa tattgtataa aagaacaagt aagagagaag cataagtata aactcgtatt
    16441 caaacatggt attgctcttt ctgagctcac acagtttctg tacggtcaat cttatttttt
    16501 aaagttttta agttattatt ttaatggtat attcacgagt acagaactgg ctgatcttga
    16561 atttatttct ttgagtaaag tatatacaat caaaaaaatc gtattggatt tttttaaaga
    16621 aaatgattat ttgaaaaata aagaaatcat tatcccagaa tttgactcgc ggaacatctt
    16681 gctagcttta gtacgttatg tcaattggga aggctatgaa aataaaaatc cgtatgtaga
    16741 agaagcttgt aacgagttga ttgattatgt agagactcat ttttttaaaa gaagatattc
    16801 ggaagatgaa aagtccttta tcatacgaga aatagagatc gcaatcggaa gaaaggcatt
    16861 tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt tatttcaatt
    16921 agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag aagatgtcta
    16981 ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg agctgttaag
    17041 aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac tgaatgaatt
    17101 gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta ttttcaagca
    17161 atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt taccggaaag
    17221 gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag aaatattgga
    17281 agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca ttcgaaaatt
    17341 cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa tagaaatttt
    17401 tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc atttgtatgc
    17461 ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag aattgcctga
    17521 tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct atcaaaatgg
    17581 attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa acatccattt
    17641 gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat gagaccttaa
    17701 ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt tgagtcctga
    17761 ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa aatgctattg
    17821 acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat tttcattaaa
    17881 agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta ttggatgcaa
    17941 tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag ttggcagaag
    18001 agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt ggtccgaatc
    18061 taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca aaggataaaa
    18121 tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt cgaaaaaatg
    18181 gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat gaaagtaaac
    18241 atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca ggtttagcta
    18301 aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt gacggctccc
    18361 taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa tccaatctaa
    18421 tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt ttatataaaa
    18481 acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt aaagcaatgg
    18541 gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc cgtttatttg
    18601 aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa aaaggtcgcg
    18661 gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca tttgatctgt
    18721 ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc atcatggatt
    18781 atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat gcagcgatac
    18841 caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat tttgacgtgg
    18901 ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag ggagtacgtc
    18961 cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta tcacatgatt
    19021 tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc tcagctagtg
    19081 accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg aacatcaaat
    19141 atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg ttcgaacaaa
    19201 cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt acagctatcg
    19261 caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa gtagcaattt
    19321 tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt agaaaaaaag
    19381 gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta gaaacattag
    19441 ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt ttgtcaggag
    19501 gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta ttaaattttg
    19561 gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa cgttatcatt
    19621 taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa taattcatta
    19681 tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca ttataaaaag
    19741 caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt tccaagaaaa
    19801 atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata cagaaaaaat
    19861 ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat caagtggtta
    19921 cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag acgtggtcct
    19981 gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca tcttgccttt
    20041 tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga cattagctta
    20101 ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt tgaacataga
    20161 atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct tttagtaaga
    20221 aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg ttacattttt
    20281 atatgaagct gtatttttat ataacattac tggaggagat attgtggata cactggaaca
    20341 aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc tacacagttc
    20401 ttttattatg gggaga
//
LOCUS       DACERL010000350        13213 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.080, whole genome shotgun sequence.
ACCESSION   DACERL010000350 DACERL010000000
VERSION     DACERL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.080"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            complement(204..2162)
                     /locus_tag="IXF54_003198"
     CDS             complement(204..2162)
                     /locus_tag="IXF54_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1406355.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="IXF54_003199"
     CDS             complement(2159..3031)
                     /locus_tag="IXF54_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1406356.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="IXF54_003200"
     CDS             complement(3373..4245)
                     /locus_tag="IXF54_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1406357.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="IXF54_003201"
     CDS             4373..4942
                     /locus_tag="IXF54_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1406358.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="IXF54_003202"
     CDS             5009..5746
                     /locus_tag="IXF54_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1406359.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="IXF54_003203"
     CDS             5977..7245
                     /locus_tag="IXF54_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1406360.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="IXF54_003204"
     CDS             7732..8196
                     /locus_tag="IXF54_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1406361.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="IXF54_003205"
     CDS             8180..8809
                     /locus_tag="IXF54_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1406362.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr"
                     /locus_tag="IXF54_003206"
     CDS             9163..10641
                     /gene="msr"
                     /locus_tag="IXF54_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1406363.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="IXF54_003207"
     CDS             10688..11134
                     /locus_tag="IXF54_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1406364.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="IXF54_003208"
     CDS             complement(11333..11848)
                     /locus_tag="IXF54_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1406365.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="IXF54_003209"
     CDS             11965..13167
                     /locus_tag="IXF54_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1406366.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DACERL010000351         3356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.138, whole genome shotgun sequence.
ACCESSION   DACERL010000351 DACERL010000000
VERSION     DACERL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.138"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            315..812
                     /gene="dfrG"
                     /locus_tag="IXF54_003210"
     CDS             315..812
                     /gene="dfrG"
                     /locus_tag="IXF54_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1406367.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(884..2836)
                     /locus_tag="IXF54_003211"
     CDS             complement(884..2836)
                     /locus_tag="IXF54_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1406368.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2843..3139)
                     /locus_tag="IXF54_003212"
     CDS             complement(2843..3139)
                     /locus_tag="IXF54_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1406369.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1039 a          576 c          552 g         1189 t
ORIGIN      
        1 gtaaatgtag tactccacta taaaggcgta tatatgcaag taagtagttc agttttggag
       61 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      121 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
      181 gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca actaacgggc
      241 aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa
      301 aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg
      361 gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta
      421 caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac
      481 ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa
      541 ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt
      601 tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa
      661 aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga
      721 atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc
      781 atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta
      841 aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta
      901 tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct
      961 ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg
     1021 caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag
     1081 ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga ataaagctat
     1141 tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag
     1201 ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt
     1261 tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt
     1321 ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact
     1381 ttgttttgaa tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt
     1441 cttttaagca ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat
     1501 cgctcaacct taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat
     1561 catcactaat cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag
     1621 cagttgtagt ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa
     1681 ttttccctaa ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt
     1741 gtggataata agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt
     1801 ctttgaattt tcttgtatct tcatttaatt catccattga aatattccaa tcataggcaa
     1861 catcagaacg gaacacatat cttgataaga cctcagcaga tacaactata tacgtttgca
     1921 tattaaggtc ttcaaacccc gtatatttct ttgaaccttg tcctttctta cggacgttat
     1981 ttaggtagta gtgcatcttg tccgtattaa gccacatctc gttaaatttc tttgtttgaa
     2041 ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg cctatacagc cactccagct
     2101 tatgatagta ggttccaact ccaattccta aaatatcaca agtacgactg actgaaaccc
     2161 ttccaacaac catcttagcg aacattggaa gaatggtgtt cttttgttgg tgataagtag
     2221 tggtttcttc tctttttggt agaacattag tgaacttctt acacgcctta cattgatagc
     2281 gttgagattt gcccctactt ttcccccttt tgtaaaattg cttcggctca ttgaatgggg
     2341 ttgattcttc ttccgagcag ccttctttat gaaattgata atctggctca acatcttgga
     2401 tgctattgat ttctataagg cgtttaattt cttcaacaac agaccaatta gataaagggg
     2461 ttactgtaca gttttgcgat acacctctgc caataggatt gtcattgcag tacatacctt
     2521 tatgaccttt atctttggaa ctaccagtca ttttatatcg acttggtttc ccttttacat
     2581 ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa cggattgatg cagtaattat
     2641 attgaatggg atattcagaa ccgttccatt taaaagacgt tggcaagaac aacattttgt
     2701 tataccttgt tgcaaatcgt tttggagtta ataaatcata ttgtttagcc ctatctttta
     2761 attcttcgtt tgaaatcgga attttaacat ctacaatttc atcttccttc gtggctaatc
     2821 ttttaagctt ggtcattgtc tatcaaccca ctctatcttt gatacccatt ttttcaacaa
     2881 tccgcttatt acttcgcttg ttggcaatcc attcaataaa cttcttatcg tctaaaatgt
     2941 tcaataagaa ttcatccact gcttcgcttt cagtacgctc tgcatattca gcgtattgct
     3001 taactatcac ccttacttgc tctgatattt cccaatccac agacttagcg ttcttatttt
     3061 ttggctcaag aaattgcata ggaaaatgca cccctttttg cttagtaaat tgtaaccatt
     3121 ttaacacacc aatttgcatt cctacaataa ttatttcacg ttcgcatttt tatatacatt
     3181 ttaacttaca ttttgtggtg taaattaaca acaaacgttg atttaacaat aaaaaacgag
     3241 tatcaaattc agttgcaatt tgatactcat ttaggtttac attttcactt tttgtactcc
     3301 aaaactgaac tacttaatat gcaagacgtt gtcttttttt tgtatattca attttg
//
LOCUS       DACERL010000352         3265 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.141, whole genome shotgun sequence.
ACCESSION   DACERL010000352 DACERL010000000
VERSION     DACERL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.141"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..803
                     /gene="rlmN"
                     /locus_tag="IXF54_003213"
     CDS             <1..803
                     /gene="rlmN"
                     /locus_tag="IXF54_003213"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1406370.1"
                     /translation="MIETVLMRQEYGLSVCVTTQVGCNIGCTFCASGLLKKNRDLTAG
                     EIVAQIMMVQHYFDERNLGERVSHVVVMGIGEPFDNYDNVMDFLHIINDAKGLAIGAR
                     HITVSTSGLAHKIKEFANNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLMEAVDE
                     YLEKTNRRITFEYIMLNQVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHDQYSR
                     SPKADVLRFYDVLKKNGVNCVIRKEHGTDIDAACGQLRSKQMKKTVAK"
     gene            complement(996..2099)
                     /locus_tag="IXF54_003214"
     CDS             complement(996..2099)
                     /locus_tag="IXF54_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1406371.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2652..3200
                     /gene="aac(6')"
                     /locus_tag="IXF54_003215"
     CDS             2652..3200
                     /gene="aac(6')"
                     /locus_tag="IXF54_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1406372.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1058 a          570 c          657 g          980 t
ORIGIN      
        1 acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac
       61 aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg
      121 atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc
      181 gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata
      241 attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg
      301 gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta
      361 ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa
      421 catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg
      481 agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa
      541 atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag
      601 catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc
      661 caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga
      721 tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa
      781 tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga
      841 gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa
      901 gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg
      961 atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc
     1021 tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct
     1081 cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt
     1141 gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc
     1201 tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag
     1261 gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca
     1321 gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca
     1381 gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga
     1441 cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac
     1501 atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa
     1561 aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca
     1621 ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag
     1681 aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa
     1741 cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact
     1801 tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca
     1861 ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga
     1921 ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa
     1981 agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac gattccattt
     2041 gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata
     2101 ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact
     2161 gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat
     2221 gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa
     2281 cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta
     2341 attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt
     2401 cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt
     2461 ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat
     2521 ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa
     2581 gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag
     2641 ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc
     2701 tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag
     2761 tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag
     2821 taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag
     2881 ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa
     2941 aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg
     3001 gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc
     3061 agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg
     3121 tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc
     3181 ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat
     3241 aagaaggaat ttacgaaaat tactt
//
LOCUS       DACERL010000353         3026 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.144, whole genome shotgun sequence.
ACCESSION   DACERL010000353 DACERL010000000
VERSION     DACERL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.144"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            47..1018
                     /locus_tag="IXF54_003216"
     CDS             47..1018
                     /locus_tag="IXF54_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ1406373.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IXF54_003217"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IXF54_003217"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ1406374.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IXF54_003218"
     CDS             2045..2653
                     /locus_tag="IXF54_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ1406375.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          813 a          636 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DACERL010000354         2440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.161, whole genome shotgun sequence.
ACCESSION   DACERL010000354 DACERL010000000
VERSION     DACERL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.161"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..75
                     /locus_tag="IXF54_003219"
     CDS             <1..75
                     /locus_tag="IXF54_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1406376.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="IXF54_003220"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="IXF54_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1406377.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2129..2296
                     /locus_tag="IXF54_003221"
     CDS             2129..2296
                     /locus_tag="IXF54_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1406378.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2356..>2440)
                     /locus_tag="IXF54_003222"
     CDS             complement(2356..>2440)
                     /locus_tag="IXF54_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1406379.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          838 a          370 c          488 g          744 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcgtttc aaaatgcagt tatggagggg atacgctatg gctgtgaaca aggattgtat
     1561 ggttggaatg tgacggactg taaaatctgt tttaagtatg gcttatacta tagccctgtt
     1621 agtaccccag cagattttcg gatgcttgct cctattgtat tggaacaagt cttaaaaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat
     1741 ctttcacgag catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg
     1801 aaaaataatg aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt
     1861 agtgatttaa ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggtac
     1921 catgttacta ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg gatagataaa
     1981 gtacgatata tgttcaataa aataacttag tgtattttat gttgttatat aaatatggtt
     2041 tcttgttaaa taagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     2101 gatagttatt ataaactaca agtggatatt gtgtcctgta tgtggaaata aaacacgatt
     2161 aaagataagg gaagatactg aattaaaaaa attccccctc tattgtccga aatgcagaca
     2221 agaaaattta attgaaataa agcagttcaa agtaactgtg attacagagc cagacgcaaa
     2281 gacgcagagc cgataaaatg agattaatac aatctcattt tatcggctct ttccgttatg
     2341 tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga
     2401 ttccattaag gatagttctt tgtctgtaaa gctatccatg
//
LOCUS       DACERL010000355         2438 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.162, whole genome shotgun sequence.
ACCESSION   DACERL010000355 DACERL010000000
VERSION     DACERL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.162"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..75
                     /locus_tag="IXF54_003223"
     CDS             <1..75
                     /locus_tag="IXF54_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1406380.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="IXF54_003224"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="IXF54_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1406381.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2108..2296
                     /locus_tag="IXF54_003225"
     CDS             2108..2296
                     /locus_tag="IXF54_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1406382.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2354..>2438)
                     /locus_tag="IXF54_003226"
     CDS             complement(2354..>2438)
                     /locus_tag="IXF54_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1406383.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          839 a          372 c          473 g          754 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat
     1561 ggttggaatg tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt
     1621 agtactccag cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat
     1741 ctttcacggg catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg
     1801 aaaaataatg aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc
     1861 aatgatttaa ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat
     1921 caggttacca ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa
     1981 gtaagatata tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt
     2041 tcttattaaa taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga
     2101 gattgttatt ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact
     2161 acgaatacga gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa
     2221 cgaaacttta attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa
     2281 gacgcagagc cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc
     2341 tgaattttac tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt
     2401 ccattaagga tagttctttg tctgtaaagc tatccatg
//
LOCUS       DACERL010000356         1572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.195, whole genome shotgun sequence.
ACCESSION   DACERL010000356 DACERL010000000
VERSION     DACERL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.195"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            17..559
                     /gene="sat4"
                     /locus_tag="IXF54_003227"
     CDS             17..559
                     /gene="sat4"
                     /locus_tag="IXF54_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1406384.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            652..1446
                     /locus_tag="IXF54_003228"
                     /note="aph(3')-IIIa"
     CDS             652..1446
                     /locus_tag="IXF54_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1406385.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          520 a          263 c          378 g          411 t
ORIGIN      
        1 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
       61 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
      121 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
      181 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
      241 tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg
      301 gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag gcataggcag
      361 cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg gactaatgct
      421 tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg gtttcaaaat
      481 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
      541 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
      601 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
      661 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
      721 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
      781 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
      841 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
      901 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
      961 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
     1021 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
     1081 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
     1141 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
     1201 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
     1261 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
     1321 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
     1381 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
     1441 ttttagtacc tagatttaga tgtctaaaaa gctttaacta caagcttttt agacatctaa
     1501 tcttttctga agtacatccg caactgtcca tactctgatg ttttatatct tttctaaaag
     1561 ttcgctagat ag
//
LOCUS       DACERL010000357         1471 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.198, whole genome shotgun sequence.
ACCESSION   DACERL010000357 DACERL010000000
VERSION     DACERL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.198"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..521
                     /locus_tag="IXF54_003229"
     CDS             <1..521
                     /locus_tag="IXF54_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1406386.1"
                     /translation="ISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAF
                     HYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYY
                     EGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRR
                     PMMLIVSAKKKM"
     gene            554..>1471
                     /gene="ant(6)"
                     /locus_tag="IXF54_003230"
     CDS             554..>1471
                     /gene="ant(6)"
                     /locus_tag="IXF54_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAQ1406387.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQGLQK"
BASE COUNT          517 a          208 c          325 g          421 t
ORIGIN      
        1 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
       61 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
      121 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
      181 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
      241 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
      301 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
      361 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
      421 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
      481 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
      541 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
      601 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
      661 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
      721 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
      781 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
      841 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
      901 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
      961 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     1021 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     1081 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     1141 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     1201 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     1261 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     1321 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     1381 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     1441 agaagagagg cgggaacagg gattacagaa a
//
LOCUS       DACERL010000358         1471 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.199, whole genome shotgun sequence.
ACCESSION   DACERL010000358 DACERL010000000
VERSION     DACERL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.199"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            <1..521
                     /locus_tag="IXF54_003231"
     CDS             <1..521
                     /locus_tag="IXF54_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1406388.1"
                     /translation="ISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAF
                     HYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYY
                     EGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRR
                     PMMLIVSAKKKM"
     gene            554..1462
                     /locus_tag="IXF54_003232"
     CDS             554..1462
                     /locus_tag="IXF54_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1406389.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
BASE COUNT          517 a          208 c          324 g          422 t
ORIGIN      
        1 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
       61 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
      121 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
      181 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
      241 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
      301 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
      361 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
      421 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
      481 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
      541 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
      601 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
      661 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
      721 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
      781 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
      841 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
      901 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
      961 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     1021 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     1081 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     1141 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     1201 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     1261 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     1321 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     1381 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     1441 agaagagagg cgggaacagt gattacagaa a
//
LOCUS       DACERL010000359         1261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.214, whole genome shotgun sequence.
ACCESSION   DACERL010000359 DACERL010000000
VERSION     DACERL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.214"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            107..841
                     /locus_tag="IXF54_003233"
     CDS             107..841
                     /locus_tag="IXF54_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ1406390.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          468 a          193 c          192 g          408 t
ORIGIN      
        1 tgatgaggtt gaacttttac acaagattgt ggacttgact aaaataagtg gttataatgg
       61 attgttaata taaacattca ttataacctc ataggagtgg gttataatga acaaaaaaaa
      121 tataaaagat agtcaaaact ttattacttc aaagcatcat ataaatgaaa ttttgagaaa
      181 tgtacattta aatacaaatg ataatattat tgagattggt tcagggaaag gtcatttcac
      241 gtttgaatta gctaaaaggt gtaattatgt aaccgccatt gaaatagatc ctaaattatg
      301 taggataact aaaaacaaac ttattgaata tgagaacttc caggttatca ataaagatat
      361 tttacaattt aagtttccta aaaataagtc atataagata tatggaaata taccctacaa
      421 tataagtaca gatataattc gaaaaattgt ttttgaaagc acagctacag aaagttattt
      481 aatagtggaa tatggatttg ctaaaaggtt gctaaataca aatcgttcac tagcactatt
      541 tttaatgaca gaagttgata tatccatatt aagtaaaatc cctagagaat actttcatcc
      601 aaaacctaga gttaatagct cgttaattgt attaaaaaga cacccttcaa aaatatcact
      661 caaagataga aaacaatatg aaaattttgt tatgaaatgg gttaacaaag aatacataaa
      721 actattttcc aaaaaccaat tttatcaagc cttaaaatat gcaagaattg acgatttaaa
      781 caatattagc tttgaacaat tcttgtctct tttcaatagc tataaattat ttaatagata
      841 agttaagggg tgcataaact gcatccctta acttatctta ttttgcttaa acaatcttgg
      901 cccgtaaact gcataactca tatgtaaatc gctccttttt aggtggcaca aatgtgaggc
      961 attttcacta tttccggcaa ttacttccat gtaaagtata acccactata ctttacttcg
     1021 taaaggtggg ctcgccgagg ggtcttgcag acggctctgt cggctctttt gagccgagta
     1081 tcagcgcacc cttatggtgc aattagctga tacaaaaaac aaagagttgg ctcatgtaat
     1141 ccaactctta aaacacaaaa aaataacccc cttggctttt cagcaggggg catatttttt
     1201 atttaattcc tcactcttta tggttctgtt gcaaagtttt ccaaaaaatc tattttagtg
     1261 t
//
LOCUS       DACERL010000360          757 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.247, whole genome shotgun sequence.
ACCESSION   DACERL010000360 DACERL010000000
VERSION     DACERL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.247"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IXF54_003234"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IXF54_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1406391.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          282 a          135 c          117 g          223 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaacattcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 gtattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgctttt
//
LOCUS       DACERL010000361          757 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium Ef_aus0069
            SAMN02318174-rid9593493.guided.248, whole genome shotgun sequence.
ACCESSION   DACERL010000361 DACERL010000000
VERSION     DACERL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN02318174
            Sequence Read Archive: SRR980574
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium Ef_aus0069
  ORGANISM  Enterococcus faecium Ef_aus0069
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705698.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705698.1
            Genome Coverage       :: 830x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 05:45:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,233
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,100
            CDSs (with protein)               :: 3,100
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 23 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium Ef_aus0069"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN02318174-rid9593493.guided.248"
                     /strain="Ef_aus0069"
                     /host="Homo sapiens"
                     /db_xref="taxon:1387037"
                     /country="Australia: Melbourne"
                     /collection_date="17-Jun-2008"
                     /collected_by="Austin Hospital"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IXF54_003235"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IXF54_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1406392.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          282 a          135 c          117 g          223 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgctttt
//
